CN114107052A - Portable stem cell amplification culture equipment capable of realizing online dynamic real-time monitoring - Google Patents
Portable stem cell amplification culture equipment capable of realizing online dynamic real-time monitoring Download PDFInfo
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- CN114107052A CN114107052A CN202111465507.0A CN202111465507A CN114107052A CN 114107052 A CN114107052 A CN 114107052A CN 202111465507 A CN202111465507 A CN 202111465507A CN 114107052 A CN114107052 A CN 114107052A
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/36—Means for collection or storage of gas; Gas holders
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/25—Greenhouse technology, e.g. cooling systems therefor
Abstract
The invention discloses a portable stem cell amplification culture device capable of realizing online dynamic real-time monitoring, which comprises: the box, be provided with the cultivation system in the box, the box is connected with the top cap, be provided with the handle on the top cap, the cultivation system includes detection device. According to the invention, the culture solution is detected in real time by the temperature sensor, the carbon dioxide concentration sensor, the oxygen concentration sensor, the urea concentration sensor and the lactic acid concentration sensor in the detection device, so that the problem of real-time dynamic monitoring of the stem cell culture process in the stem cell culture process is solved, and the defect that the existing stem cell amplification culture equipment is not portable is also solved by arranging the lifting handle on the top cover.
Description
Technical Field
The invention relates to the technical field of stem cell amplification culture, in particular to a portable stem cell amplification culture device capable of realizing online dynamic real-time monitoring.
Background
The function of the stem cell population is to control and maintain the regeneration of cells. Generally, between stem cells and their terminally differentiated progeny, there is an intermediate population of progenitor cells, called "committed progenitors," which have limited expansion capacity and limited differentiation potential. These cell populations function to increase the number of differentiated cells produced after each division of the stem cells.
Functionally, stem cells are cells with multipotentiality and self-renewal capacity, are the most primitive cells at the top of the cell line origin, and are capable of differentiating in vivo to give rise to cells of a particular tissue type. In adult organs, stem cells can repair tissue by constantly dividing, or are in a quiescent state as in mammalian brain tissue. Stem cells are capable of expanding their numbers by dividing symmetrically during their development, or self-renewing and producing more progenitor cells of different differentiation types by dividing asymmetrically.
In the current research fields of clinical medicine, life science, cell biology and the like, the mass amplification and cultivation of stem cells are very important works. However, the stem cells can be differentiated during the expansion culture process, so that the dryness is lost, and the expansion culture of the stem cells fails. In order to ensure that the stem cells are not differentiated in the culture process, the culture process of the stem cells needs to be dynamically monitored in real time, and the conventional stem cell amplification culture device does not have the function.
Disclosure of Invention
The invention relates to a portable stem cell amplification culture device capable of realizing online dynamic real-time monitoring, which is used for solving the problems of the prior art proposed in the background art: in order to ensure that the stem cells are not differentiated in the culture process, the culture process of the stem cells needs to be dynamically monitored in real time, and the conventional stem cell amplification culture device does not have the function.
But portable stem cell amplification culture equipment of online dynamic real-time supervision includes: the box, be provided with the cultivation system in the box, the box is connected with the top cap, be provided with the handle on the top cap, the cultivation system includes detection device.
Preferably, a display and an operation button are arranged on the top cover, and the display and the operation button are electrically connected with the culture system.
Preferably, a travel switch is arranged on the lower surface of the top cover, an electromagnet is fixed at the opening of the box body, and the travel switch is electrically connected with the electromagnet.
Preferably, the culture system includes the incubator, the incubator passes through a plurality of support piece and connects on the base, the base set up in the box, support piece lower extreme and base lower extreme fixed connection.
Preferably, support piece upper portion sets up first pivot, incubator and first pivot fixed connection, be fixed with the motor on the base, motor and first gear connection, the incubator surface is provided with the tooth, the support piece middle part is rotated and is connected with the second gear, second gear and tooth engagement, just second gear and first gear engagement.
Preferably, the incubator both sides all are provided with the connecting tube, and the connecting tube of both sides is connected with first solenoid valve, second solenoid valve one end respectively, the second solenoid valve other end passes through the gaseous collection jar of intercommunication pipe connection, gaseous collection jar connection culture solution storage jar.
Preferably, the detection device includes: the detection assembly, the first solenoid valve other end passes through the intercommunication tube coupling detection assembly, culture solution storage jar, gaseous collection jar all are fixed in on the base.
Preferably, the detection assembly comprises a detection box, and the detection box is internally provided with: at least one of a temperature sensor, a carbon dioxide concentration sensor, an oxygen concentration sensor, a urea concentration sensor and a lactic acid concentration sensor;
be provided with carbon dioxide jar, oxygen jar, nitrogen gas jar in the gaseous collection jar, carbon dioxide jar, oxygen jar, nitrogen gas jar parallel connection the intercommunication pipeline.
Preferably, a plurality of groups of buffer devices are arranged on the lower peripheral side of the base, and each buffer device comprises:
the device comprises a sliding rail, a trapezoidal block, a fixed shell, a sliding piece, a first connecting piece, a triangular plate, a second connecting piece, a third connecting piece, a fourth connecting piece, a second gear, a first spring, a stop block, a third gear, a fifth connecting piece, a sixth connecting piece, a second spring, a seventh connecting piece, a third spring and a balancing rod;
the trapezoidal block is arranged below the side of the base, the inclined surface of the trapezoidal block is in contact with the base, the trapezoidal block is fixed on the sliding piece, the sliding piece is connected to the sliding rail in a sliding manner, and the sliding rail is fixed on the inner lower surface of the box body;
the first angle of the triangular plate is rotationally connected to the first connecting piece, the first connecting piece is slidably connected to the sliding piece, the second angle of the triangular plate is rotationally connected to the fixed shell through the second connecting piece, and the third angle of the triangular plate is rotationally connected to the second gear through the third connecting piece;
the side of the triangular plate between the second corner and the third corner is connected with one end of a first spring, the other end of the first spring is connected with the stop block, the stop block is fixed on the lower surface of the fixed shell, and the fixed shell is fixed on the inner lower surface of the box body;
the second gear is rotationally connected to the fixed shell through the fourth connecting piece, the second gear is meshed with the third gear, the third gear is sector-shaped and is rotationally connected to the fixed shell through the fifth connecting piece, and the toothless end of the third gear is fixed with the middle section of the balancing rod;
and two ends of the balance rod are respectively connected with one ends of the second spring and the third spring through the sixth connecting piece and the seventh connecting piece, and the other ends of the second spring and the third spring are fixed with the fixed shell.
Preferably, be provided with temperature regulation apparatus on the base, temperature regulation apparatus includes:
the first fixing part, the first connecting rod, the fourth spring, the second connecting rod, the first articulated shaft, the second articulated shaft, the third connecting rod, the first slider, the fourth articulated shaft, the fifth articulated shaft, the first temperature regulating part, the fourth connecting rod, the fifth articulated shaft, the fifth connecting rod, the second slider, the sixth articulated shaft, the sixth connecting rod, the seventh articulated shaft, the second fixing part, the eighth articulated shaft, the linkage gear, the rack, the second temperature regulating part, the sliding frame, the driving rod, the linear motor, the elastic roller, the ninth articulated shaft, the sleeve, the fifth spring, the sleeve shaft, the tenth articulated shaft and the third temperature regulating part;
the linear motor is fixed on the upper surface of the base, the output end of the linear motor is fixed at one end of the driving rod, the sliding frame is in a n shape, and the other end of the driving rod is fixed at the lower end of the sliding frame;
the first fixing piece is fixed on the upper surface of the sliding frame, the second connecting rod is in a 7 shape, one end of the second connecting rod is connected with one end of the first connecting rod, and the bent part of the second connecting rod is connected with the first fixing piece through the second hinge shaft;
the connecting end of the first connecting rod and the second connecting rod is connected to one end of a fourth spring, the other end of the fourth spring is fixed on the upper surface of the base, and the other end of the first connecting rod is in sliding connection with the top cover through the first hinge shaft;
the other end of the second connecting rod is connected with one end of a third connecting rod and one end of a fourth connecting rod through the third hinge shaft, the other end of the third connecting rod is connected with the first sliding block through the fourth hinge shaft, and the first sliding block is connected to the first fixing piece in a sliding mode;
the upper end of the first fixing piece is connected with the first temperature adjusting piece through the fifth hinge shaft, the first temperature adjusting piece is provided with a bulge, and the bulge of the first temperature adjusting piece is contacted with the top end of the first sliding block;
the other end of the fourth connecting rod is connected with one end of the fifth connecting rod and one end of a sixth connecting rod through the fifth hinge shaft, the other end of the sixth connecting rod is connected with the second fixing piece through the seventh hinge shaft, and the second fixing piece is fixed on the upper surface of the base;
the other end of the fifth connecting rod is connected with the second sliding block through the eighth hinge shaft, the second sliding block is connected with the second fixing piece in a sliding mode, meshing teeth are arranged on one side of the second sliding block, the meshing teeth of the second sliding block are meshed with the linkage gear, the rack is connected with the second fixing piece in a sliding mode, and the rack is meshed with the linkage gear;
the top end of the second fixing piece is connected with the second temperature adjusting piece through the sixth hinge shaft, the second temperature adjusting piece is provided with a bulge, and the bulge of the second temperature adjusting piece is contacted with the top end of the rack;
the outer edges of the first temperature regulating part and the second temperature regulating part are connected with the sleeve shaft through the tenth articulated shaft, the sleeve shaft is sleeved with the fifth spring, the sleeve shaft and the fifth spring are inserted into the sleeve, and the sleeve is connected with the first fixing part and the second fixing part through the ninth articulated shaft;
the third temperature adjusting part is connected between the first fixing part and the second fixing part;
heating pipes are arranged in the first temperature regulating part, the second temperature regulating part and the third temperature regulating part;
the first temperature regulating part, the second temperature regulating part and the third temperature regulating part face one side of the incubator and are respectively provided with the elastic roller.
Drawings
FIG. 1 is a schematic front view of the main structure of the present invention;
FIG. 2 is a perspective view of the main structure of the present invention;
FIG. 3 is a schematic front view of the culture system of the present invention;
FIG. 4 is a schematic side view of the temperature control device of the present invention;
fig. 5 is a front view schematically illustrating a structure of a buffering device according to the present invention.
In the figure: 1. a box body; 2. a culture system; 3. a top cover; 4. a buffer device; 5. a temperature adjustment device; 6. a handle; 7. a display; 8. an operation button; 9. a travel switch; 10. an electromagnet; 201. a base; 202. a motor; 203. a support member; 204. a culture solution storage tank; 205. a first gear; 206. a detection component; 207. a second gear; 208. a first solenoid valve; 209. an incubator; 210. a second solenoid valve; 211. a communicating pipeline; 212. a gas collection tank; 213. a first rotating shaft; 214. teeth; 401. a slide rail; 402. a trapezoidal block; 403. a stationary housing; 404. a slider; 405. a first connecting member; 406. a set square; 407. a second connecting member; 408. a third connecting member; 409. a fourth connecting member; 410. a second gear; 411. a first spring; 412. a stopper; 413. a third gear; 414. a fifth connecting member; 415. a sixth connecting member; 416. a second spring; 417. a seventh connecting member; 418. a third spring; 501. a first fixing member; 502. a first link; 503. a fourth spring; 504. a second link; 505. a first hinge shaft; 506. a second hinge shaft; 507. a third hinge shaft; 508. a third link; 509. a first slider; 510. a fourth hinge shaft; 511. a fifth hinge shaft; 512. a first temperature adjustment member; 513. a fourth link; 514. a fifth hinge shaft; 515. a fifth link; 516. a second slider; 517. a sixth hinge shaft; 518. a sixth link; 519. a seventh hinge shaft; 520. a second fixing member; 521. an eighth hinge shaft; 522. a linkage gear; 523. a rack; 524. a second temperature adjustment member; 525. a carriage; 526. a drive rod; 527. a linear motor; 528. an elastic roller; 529. a ninth hinge shaft; 530. a sleeve; 531. a fifth spring; 532. a quill; 533. a tenth hinge shaft; 534. a third temperature adjustment member.
Detailed Description
The description of the present invention as to "first", "second", etc. is for descriptive purposes only, and not for purposes of particular ordinal or sequential meaning, nor for limitations, and is intended to distinguish between components or operations that are described in the same technical language, and is not intended to indicate or imply relative importance or imply the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, technical solutions and technical features between various embodiments can be combined with each other, but must be realized by a person skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should be considered to be absent and not be within the protection scope of the present invention.
Example 1
Referring to fig. 1-3, an embodiment of the present invention is shown: but portable stem cell amplification culture equipment of online dynamic real-time supervision includes: the box 1, be provided with cultivation system 2 in the box 1, box 1 is connected with top cap 3, be provided with handle 6 on the top cap 3, cultivation system 2 includes detection device.
Preferably, the top cover 3 is provided with a display 7 and an operation button 8, and the display 7 and the operation button 8 are electrically connected with the culture system 2.
Preferably, a travel switch 9 is arranged on the lower surface of the top cover 3, an electromagnet 10 is fixed at an opening of the box body 1, and the travel switch 9 is electrically connected with the electromagnet 10.
Preferably, the culture system 2 comprises an incubator 209, the incubator 209 is connected to the base 201 through a plurality of supporting members 203, the base 201 is disposed in the case 1, and the lower end of the supporting members 203 is fixedly connected with the lower end of the base 201.
The working principle and the beneficial effects of the technical scheme are as follows: when the device is used, the environment in the culture system 2 is preset through the operation button 8, so that the environment is suitable for stem cell amplification culture, stem cells are placed in the culture box 209 for amplification culture, the detection device meets the real-time detection of the stem cell amplification culture environment, the understanding of the stem cell amplification culture condition is facilitated, and the problem that the conventional stem cell amplification culture device does not have the function of real-time dynamic monitoring on the culture process of the stem cells is solved;
the handle 6 arranged on the top cover 3 also solves the defect that the existing stem cell amplification culture equipment is not portable.
Example 2
Referring to fig. 3, on the basis of the above embodiment 1, the upper portion of the supporting member 203 is provided with a first rotating shaft 213, the incubator 209 is fixedly connected to the first rotating shaft 213, the base 201 is fixed with the motor 202, the motor 202 is connected to the first gear 205, the outer surface of the incubator 209 is provided with teeth 214, the middle portion of the supporting member 203 is rotatably connected to a second gear 207, the second gear 207 is engaged with the teeth 214, and the second gear 207 is engaged with the first gear 205.
The working principle and the beneficial effects of the technical scheme are as follows: the device is provided with a motor 202, when the motor 202 rotates, a first gear 205 is driven to rotate, the first gear 205 is meshed with a second gear 207, a second gear 207 is meshed with teeth outside an incubator 209, the incubator 209 is driven to rotate, the incubator 209 is enabled to simulate a microgravity state, the simulated microgravity state can promote the proliferation and the differentiation inhibition of stem cells, the simulated microgravity environment can promote the aggregation of seed cells by creating a three-dimensional culture environment with low turbulence and low shearing force, the seed cells are guided to be uniformly distributed in a support material, and simultaneously, in the environment simulating microgravity, as an object is less influenced by effective gravity, the support material is easier to form a compound with larger size and uniform distribution, the proliferation efficiency of the stem cells in the simulated microgravity environment is higher than that in a plane, the differentiation of the stem cells in the proliferation culture process can be effectively inhibited, namely, the method prevents the difference of the formed form and function between individual cells and changes the original physiological state of the stem cells.
Example 3
Referring to fig. 2-3, on the basis of the above embodiment 1 or 2, the two sides of the incubator 209 are respectively provided with a connecting pipeline, the connecting pipelines at the two sides are respectively connected with one end of a first electromagnetic valve 208 and one end of a second electromagnetic valve 210, the other end of the second electromagnetic valve 210 is connected with a gas collection tank 212 through a communication pipeline 211, and the gas collection tank 212 is connected with the culture solution storage tank 204.
Preferably, the detection device includes: the other end of the first electromagnetic valve 208 is connected with the detection component 206 through a communication pipeline 211, and the detection component 206, the culture solution storage tank 204 and the gas collection tank 212 are all fixed on the base 201.
Preferably, the detection assembly 206 comprises a detection box, and the detection box is provided with: at least one of a temperature sensor, a carbon dioxide concentration sensor, an oxygen concentration sensor, a urea concentration sensor and a lactic acid concentration sensor;
be provided with carbon dioxide jar, oxygen jar, nitrogen gas jar in the gaseous collection jar 212, carbon dioxide jar, oxygen jar, nitrogen gas jar parallel connection intercommunication pipeline 211.
Wherein, still can set up detection device as required in the incubator for detect incubator internal environment and/or stem cell's culture state, detection device can include: a camera is provided.
The working principle and the beneficial effects of the technical scheme are as follows: the culture solution in the culture solution storage tank 204 is injected into the culture box 209, in the process that the culture solution in the culture solution storage tank 204 is injected into the culture box 209, various gases in the gas collection tank 212 are introduced into the culture solution, so that the dissolved amount of each gas in the culture solution meets the condition of stem cell amplification, the culture solution entering the culture box 209 enters the detection device when the culture solution is not available, the culture solution is detected by a temperature sensor, a carbon dioxide concentration sensor, an oxygen concentration sensor, a urea concentration sensor and a lactic acid concentration sensor which are arranged in a detection assembly 206 of the detection device, and detection data are displayed on a display 7 in real time, so that the problem that the culture process of the stem cells is not dynamically monitored in real time in the culture process of the stem cells is solved, and the data are compared with the data preset by an operation button 8 to regulate the environmental conditions in the culture system 2 again, by establishing the negative feedback mechanism, the environment of the culture system 2 is ensured to be suitable for the amplification culture of the stem cells.
Example 4
Referring to fig. 1 and 5, on the basis of any one of the above embodiments 1 to 3, a plurality of sets of buffer devices 4 are disposed on the lower peripheral side of the base 201, and each buffer device 4 includes:
the device comprises a sliding rail 401, a trapezoidal block 402, a fixed shell 403, a sliding piece 404, a first connecting piece 405, a triangular plate 406, a second connecting piece 407, a third connecting piece 408, a fourth connecting piece 409, a second gear 410, a first spring 411, a stop 412, a third gear 413, a fifth connecting piece 414, a sixth connecting piece 415, a second spring 416, a seventh connecting piece 417, a third spring 418 and a balance rod 419;
the trapezoidal block 402 is arranged below the side of the base 201, the inclined surface of the trapezoidal block 402 is in contact with the base 201, the trapezoidal block 402 is fixed on the sliding piece 404, the sliding piece 404 is slidably connected with the sliding rail 401, and the sliding rail 401 is fixed on the inner lower surface of the box body 1;
the first corner of the triangular plate 406 is rotatably connected to the first connecting member 405, the first connecting member 405 is slidably connected to the sliding member 404, the second corner of the triangular plate 406 is rotatably connected to the fixed housing 403 through the second connecting member 407, and the third corner of the triangular plate 406 is rotatably connected to the second gear 410 through the third connecting member 408;
one end of a first spring 411 is connected to the side of the triangular plate 406 between the second corner and the third corner, the other end of the first spring 411 is connected to the stopper 412, the stopper 412 is fixed on the lower surface of the fixed shell 403, and the fixed shell 403 is fixed on the inner lower surface of the box body 1;
the second gear 410 is rotatably connected to the fixed housing 403 through the fourth connecting member 409, the second gear 410 is meshed with the third gear 413, the third gear 413 is fan-shaped and is rotatably connected to the fixed housing 403 through the fifth connecting member 414, and the toothless end of the third gear 413 is fixed to the middle section of the balance bar 419;
two ends of the balance bar 419 are respectively connected with one ends of the second spring 416 and the third spring 418 through the sixth connecting piece 415 and the seventh connecting piece 417, and the other ends of the second spring 416 and the third spring 418 are fixed with the fixed shell 403.
The working principle and the beneficial effects of the technical scheme are as follows: the buffer device 4 can effectively relieve the influence of vibration generated in the carrying and moving processes on stem cell amplification culture equipment and prevent the amplification culture of stem cells;
when vibration occurs, the base 201 is in contact with the inclined surface of the trapezoidal block 402, when the base 201 moves downwards, the trapezoidal block 402 moves towards two sides to drive the sliding piece 404 to move on the sliding rail 401, and the triangular plate 406 rotates around the second connecting piece 407, at the moment, the first spring 411 deforms, and the movement of the base 201 is buffered for the first time;
the triangular plate 406 is connected with the second gear 410 through the third connecting piece 408, when the triangular plate 406 rotates, the second gear 410 rotates along with the triangular plate, and the third gear 413 meshed with the second gear 410 rotates around the fifth connecting piece 414, and when the third gear 413 rotates, the balancing rod 419 drives the second spring 416 and the third spring 418 to deform, so that the movement of the base 201 is buffered for the second time, and the influence of vibration generated by the stem cell amplification culture equipment in the carrying and moving processes on the stem cell amplification culture equipment is reduced;
the second spring 416 and the third spring 418 are arranged, and the second spring 416 and the third spring 418 are fixed on the fixed shell 403 in a right angle, so that the stability of secondary buffering is ensured, and the service life of the buffering device 4 is prolonged;
the trapezoidal block 402 is arranged, the stability of the base 201 when the base 201 is static is guaranteed due to the inclined surface of the trapezoidal block, when the base 201 vibrates, force generated by vibration of the base 201 is diverted, buffering is carried out on the side of the base 201, the longitudinal space of equipment is effectively reduced, and the lower side of the trapezoidal block 402 is rectangular, so that the bearing capacity of the trapezoidal block 402 is effectively guaranteed;
the energy which causes the vibration of the base 201 is collected through the spring energy storage of the first spring 411, the second spring 416 and the third spring 418 twice, and is slowly released later, so that the shaking generated during the displacement is effectively reduced, and the carrying stability is ensured;
through the vibrations that slow down base 201, guaranteed the work condition that sets up each part on base 201, alleviated through the situation of rocking to each part, reduced the shearing force that each part received, played fine guard action to each part, be favorable to lifting means's life, reduce the maintenance frequency.
Example 5
Referring to fig. 1 and 3 to 4, on the basis of any one of the above embodiments 1 to 4, a temperature adjusting device 5 is disposed on the base 201, and the temperature adjusting device 5 includes:
a first fixing member 501, a first link 502, a fourth spring 503, a second link 504, a first hinge shaft 505, a second hinge shaft 506, a third hinge shaft 507, a third link 508, a first slider 509, a fourth hinge shaft 510, a fifth hinge shaft 511, a first temperature adjusting member 512, a fourth link 513, a fifth hinge shaft 514, a fifth link 515, a second slider 516, a sixth hinge shaft 517, a sixth link 518, a seventh hinge shaft 519, a second fixing member 520, an eighth hinge shaft 521, a link gear 522, a rack 523, a second temperature adjusting member 524, a carriage 525, a driving lever 526, a linear motor 527, an elastic roller 528, a ninth hinge shaft 529, a sleeve 530, a fifth spring 531, a sleeve shaft 532, a tenth hinge shaft 533, and a third temperature adjusting member 534;
the linear motor 527 is fixed on the upper surface of the base 201, the output end of the linear motor 527 is fixed at one end of the driving rod 526, the sliding frame 525 is in an n shape, and the other end of the driving rod 526 is fixed at the lower end of the sliding frame 525;
the first fixing member 501 is fixed on the upper surface of the sliding frame 525, the second connecting rod 504 is in a 7 shape, one end of the second connecting rod 504 is connected with one end of the first connecting rod 502, and the bent part of the second connecting rod 504 is connected with the first fixing member 501 through the second hinge shaft 506;
the connection end of the first link 502 and the second link 504 is connected to one end of the fourth spring 503, the other end of the fourth spring 503 is fixed on the upper surface of the base 201, and the other end of the first link 502 is slidably connected to the top cover 3 through the first hinge shaft 505;
the other end of the second link 504 is connected to one end of a third link 508 and one end of a fourth link 513 through a third hinge shaft 507, the other end of the third link 508 is connected to the first slider 509 through a fourth hinge shaft 510, and the first slider 509 is slidably connected to the first fixing member 501;
the upper end of the first fixing member 501 is connected to the first temperature adjusting member 512 through the fifth hinge shaft 511, the first temperature adjusting member 512 is provided with a protrusion, and the protrusion of the first temperature adjusting member 512 is in contact with the top end of the first slider 509;
the other end of the fourth link 513 is connected to one end of the fifth link 515 and one end of the sixth link 518 through the fifth hinge shaft 514, the other end of the sixth link 518 is connected to the second fixing element 520 through the seventh hinge shaft 519, and the second fixing element 520 is fixed to the upper surface of the base 201;
the other end of the fifth link 515 is connected to the second slider 516 through the eighth hinge shaft 521, the second slider 516 is slidably connected to the second fixing member 520, one side of the second slider 516 is provided with engaging teeth, the engaging teeth of the second slider 516 are engaged with the linkage gear 522, the rack 523 is slidably connected to the second fixing member 520, and the rack 523 is engaged with the linkage gear 522;
the top end of the second fixing member 520 is connected to the second temperature adjusting member 524 through the sixth hinge shaft 517, the second temperature adjusting member 524 is provided with a protrusion, and the protrusion of the second temperature adjusting member 524 is in contact with the top end of the rack 523;
the outer edges of the first temperature adjusting element 512 and the second temperature adjusting element 524 are connected to the sleeve shaft 532 through the tenth hinge shaft 533, the fifth spring 531 is sleeved outside the sleeve shaft 532, the sleeve shaft 532 and the fifth spring 531 are inserted into the sleeve 530, and the sleeve 530 is connected to the first fixing element 501 and the second fixing element 520 through the ninth hinge shaft 529;
the third temperature adjusting member 534 is connected between the first fixing member 501 and the second fixing member 520;
heating pipes are arranged in the first temperature adjusting part 512, the second temperature adjusting part 524 and the third temperature adjusting part 534;
the elastic rollers 528 are arranged on the sides of the first temperature adjusting member 512, the second temperature adjusting member 524 and the third temperature adjusting member 534 facing the incubator 209.
The working principle and the beneficial effects of the technical scheme are as follows: the temperature adjusting device 5 is used for controlling the temperature of the incubator 209, and can heat the temperature conditions of all parts of the incubator 209 in a targeted manner, so that the stem cell amplification culture is facilitated;
when the top cover 3 is closed, the first link 502 is driven to move downward, the fourth spring 503 is compressed, the second link 504 rotates around the second hinge shaft 506, the first slider 509 is driven to move upward through the third link 508, and the first temperature adjusting member 512 rotates clockwise around the fifth hinge shaft 511;
when the second link 504 rotates around the second hinge shaft 506, the fourth link 513 is driven to move left, the fifth link 515 enables the second slider 516 to move down, and because the second slider 516 is engaged with the rack 523 through the linkage gear 522, when the second slider 516 moves down, the rack 523 moves up, and the second temperature adjusting member 524 rotates counterclockwise around the sixth hinge shaft 517;
when the first temperature adjusting part 512 rotates clockwise and the second temperature adjusting part 524 rotates counterclockwise, a surrounding for the incubator 209 is formed by matching with the third temperature adjusting part 534, and the first temperature adjusting part 512, the second temperature adjusting part 524 and the third temperature adjusting part 534 are all fixed on the sliding frame 525, when the sliding frame 525 slides under the action of the linear motor, the first temperature adjusting part 512 and the second temperature adjusting part 524 heat different positions of the incubator 209, so as to realize the temperature control of the incubator 209;
the elastic roller 528 can ensure that the first temperature adjusting part 512, the second temperature adjusting part 524 and the third temperature adjusting part 534 move relative to the incubator 209, and can apply a certain force to the incubator 209 to ensure the stability of the incubator 209, and meanwhile, the elastic action of the elastic roller 528 can prevent the first temperature adjusting part 512, the second temperature adjusting part 524 and the third temperature adjusting part 534 from excessively deviating to cause irreversible damage to the incubator 209;
when top cap 3 is closed, can make first temperature regulation spare 512, second temperature regulation spare 524 to the incubator 209 around, can play to carry out certain limit effect to incubator 209 carrying the in-process, reduce the removal trend of incubator 209, avoid influencing stem cell's amplification and cultivate, simultaneously, also can increase the life of equipment.
Example 6
The method according to any one of embodiments 1 to 5, further comprising a stem cell expansion culture stability detection device, the stem cell expansion culture stability detection device comprising:
a temperature sensor, which is arranged outside the incubator 209 and is used for detecting the working temperature of the incubator 209;
an angular velocity sensor provided on the incubator 209 for detecting an angular velocity of the incubator 209;
a timer for recording a single use time of the incubator 209;
a counter for recording the number of uses of the incubator 209;
the alarm is positioned on the box body 1;
the controller, the controller respectively with temperature sensor, angular velocity sensor, time-recorder, counter and alarm electric connection, the controller is based on temperature sensor, angular velocity sensor, time-recorder, counter control alarm work includes:
step 1: the controller obtains the status characteristic index of the incubator 209 based on the temperature sensor, the timer, the counter, and the formula (1):
wherein A is a state characteristic index of the incubator 209, T is a working temperature of the incubator 209 detected by the temperature sensor, and delta is a precision of the temperature sensorCoefficient of degree, T0Is the thermodynamic temperature corresponding to zero degrees centigrade, sigma the effective stress concentration coefficient of the incubator 209 refers to the ratio of the fatigue limit of the smooth sample to the fatigue limit of the notched sample, t is the single use time of the incubator 209 recorded by the timer, t is0N is the number of uses of the incubator 209 recorded by the counter, exp is an exponential function with a natural constant e as the base;
step 2: the controller calculates an incubator 209 stability index based on the angular velocity sensor and equation (2):
wherein B is an incubator 209 stability index, m is the weight of the incubator 209, G is the tensile strength of the incubator 209 material, S is the surface area of the incubator 209, d is the incubator 209 length, C is the cross-sectional area of the incubator 209, θ is the angular velocity of the incubator 209 detected by the angular velocity sensor, e is a natural constant, which takes a value of 2.72, sin is a sine function, cos is a cosine function, G is the gravitational acceleration; ln is a natural logarithm;
when the stability index of the incubator 209 exceeds a preset reference value range, the controller controls an alarm to give an alarm.
In the formula (I), the compound is shown in the specification,
for the basic fatigue property index of the incubator 209 based on temperature,
a coefficient for correcting the basic fatigue characteristic index of the incubator 209 based on the total working time of the handle 6,
the fatigue characteristic index of the incubator 209 is shown based on the specification and stress of the incubator 209The size direction gives the incubator 209 stability index.
It is assumed that the operating temperature T of the incubator 209 detected by the temperature sensor is 298.15K, the accuracy coefficient δ of the temperature sensor is 0.99, and the thermodynamic temperature T corresponding to zero degrees centigrade is 0.990273.15K, the effective stress concentration coefficient sigma of the incubator 209 is 10.54, the single use time t of the incubator 209 recorded by a timer is 20min, and the unit time t is0When the number of times n of use of the incubator 209 recorded in the counter is 100 at 1min, the state characteristic index a of the incubator 209 is 6.586 calculated as described above.
The weight m of the incubator 209 is 5kg, the tensile strength G of the material of the incubator 209 is 20MPa, and the surface area S of the incubator 209 is 2000cm2The length d of the incubator 209 is 15cm, and the cross-sectional area C of the incubator 209 is 100cm2The angular velocity theta of the incubator 209 detected by the angular velocity sensor is 10rad/s, the stability index B of the incubator 209 is 2.625 (three decimal places are taken) calculated by the formula (2), the stability index B of the incubator 209 obtained by calculation is 2.625, and the preset reference value range is not exceeded by 2-5, and at the moment, the controller does not control the alarm to give an alarm prompt.
The beneficial effects of the above technical scheme are: by providing a temperature sensor for detecting the operating temperature of the incubator 209, providing a timer for recording the single use time of the incubator 209, providing a counter for recording the number of times the incubator 209 is used, calculating the status characteristic index of the incubator 209 by detecting the operating temperature of the incubator 209, recording the single use time, the number of times of the incubator 209, and formula (1), and at the same time, providing an angular velocity sensor for detecting the angular velocity of the incubator 209, and then calculating the stability index of the incubator 209 according to the calculation result of formula (1), the angular velocity of the incubator 209 detected by the angular velocity sensor, and formula (2), when the stability index of the incubator 209 is beyond a preset reference value range, the controller controls an alarm to notify the user of the maintenance of the apparatus, the stability of stem cell amplification culture is guaranteed, the alarm is controlled to give an alarm through the controller, related workers are informed of overhauling in time, and the intelligence of the device is improved.
It will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, and that the present invention may be embodied in other specific forms without departing from the spirit or essential attributes thereof. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (10)
1. But portable stem cell amplification culture equipment of online dynamic real-time supervision includes: box (1), its characterized in that: the cultivation system is characterized in that a cultivation system (2) is arranged in the box body (1), the box body (1) is connected with a top cover (3), a lifting handle (6) is arranged on the top cover (3), and the cultivation system (2) comprises a detection device.
2. The portable stem cell expansion and culture equipment capable of being monitored on line and dynamically in real time according to claim 1, wherein: the top cover (3) is provided with a display (7) and an operating button (8), and the display (7) and the operating button (8) are electrically connected with the culture system (2).
3. The portable stem cell expansion and culture equipment capable of being monitored on line and dynamically in real time according to claim 1, wherein: the lower surface of the top cover (3) is provided with a travel switch (9), an electromagnet (10) is fixed at the opening of the box body (1), and the travel switch (9) is electrically connected with the electromagnet (10).
4. The portable stem cell expansion and culture equipment capable of being monitored on line and dynamically in real time according to claim 1, wherein:
culture system (2) includes incubator (209), incubator (209) are connected on base (201) through a plurality of support piece (203), base (201) set up in box (1), support piece (203) lower extreme and base (201) lower extreme fixed connection.
5. The portable stem cell expansion and culture equipment capable of being monitored on line and dynamically in real time according to claim 4, wherein:
support piece (203) upper portion sets up first pivot (213), incubator (209) and first pivot (213) fixed connection, be fixed with motor (202) on base (201), motor (202) are connected with first gear (205), incubator (209) surface is provided with tooth (214), support piece (203) middle part is rotated and is connected with second gear (207), second gear (207) and tooth (214) meshing, just second gear (207) and first gear (205) meshing.
6. The portable stem cell expansion and culture equipment capable of being monitored on line and dynamically in real time according to claim 4, wherein:
incubator (209) both sides all are provided with connecting tube, and the connecting tube of both sides is connected with first solenoid valve (208), second solenoid valve (210) one end respectively, gaseous collection jar (212) is connected through intercommunication pipeline (211) to second solenoid valve (210) other end, culture solution storage jar (204) is connected in gaseous collection jar (212).
7. The portable stem cell expansion and culture equipment capable of being monitored on line and dynamically in real time according to claim 6, wherein: the detection device includes: the detection assembly (206), the other end of first solenoid valve (208) is connected through communicating pipe (211) detection assembly (206), culture solution storage jar (204), gaseous collection jar (212) all are fixed in on base (201).
8. The portable stem cell expansion and culture equipment capable of being monitored on line and dynamically in real time according to claim 7, wherein: the detection assembly (206) comprises a detection box, and the detection box is internally provided with: at least one of a temperature sensor, a carbon dioxide concentration sensor, an oxygen concentration sensor, a urea concentration sensor and a lactic acid concentration sensor;
be provided with carbon dioxide jar, oxygen jar, nitrogen gas jar in gaseous collection jar (212), carbon dioxide jar, oxygen jar, nitrogen gas jar parallel connection intercommunication pipeline (211).
9. The portable stem cell expansion and culture equipment capable of being monitored on line and dynamically in real time according to claim 4, wherein: all sides are provided with a plurality of groups buffer (4) down base (201), buffer (4) include:
the device comprises a sliding rail (401), a trapezoidal block (402), a fixed shell (403), a sliding piece (404), a first connecting piece (405), a triangular plate (406), a second connecting piece (407), a third connecting piece (408), a fourth connecting piece (409), a second gear (410), a first spring (411), a stop block (412), a third gear (413), a fifth connecting piece (414), a sixth connecting piece (415), a second spring (416), a seventh connecting piece (417), a third spring (418) and a balancing rod (419);
the trapezoidal block (402) is arranged below the side of the base (201), the inclined surface of the trapezoidal block (402) is in contact with the base (201), the trapezoidal block (402) is fixed on the sliding piece (404), the sliding piece (404) is connected to the sliding rail (401) in a sliding manner, and the sliding rail (401) is fixed on the inner lower surface of the box body (1);
the first angle of the triangular plate (406) is rotatably connected to the first connecting piece (405), the first connecting piece (405) is slidably connected to the sliding piece (404), the second angle of the triangular plate (406) is rotatably connected to the fixed shell (403) through the second connecting piece (407), and the third angle of the triangular plate (406) is rotatably connected to the second gear (410) through the third connecting piece (408);
the side of the triangular plate (406) between the second corner and the third corner is connected with one end of a first spring (411), the other end of the first spring (411) is connected with a stopper (412), the stopper (412) is fixed on the lower surface of the fixed shell (403), and the fixed shell (403) is fixed on the inner lower surface of the box body (1);
the second gear (410) is rotatably connected to the fixed housing (403) through the fourth connecting piece (409), the second gear (410) is meshed with the third gear (413), the third gear (413) is fan-shaped and is rotatably connected to the fixed housing (403) through the fifth connecting piece (414), and the toothless end of the third gear (413) is fixed to the middle section of the balance bar (419);
two ends of the balance rod (419) are respectively connected with one ends of the second spring (416) and the third spring (418) through the sixth connecting piece (415) and the seventh connecting piece (417), and the other ends of the second spring (416) and the third spring (418) are fixed with the fixed shell (403).
10. The portable stem cell expansion and culture equipment capable of being monitored on line and dynamically in real time according to claim 4, wherein: be provided with temperature regulation apparatus (5) on base (201), temperature regulation apparatus (5) include:
first fixing part (501), first connecting rod (502), fourth spring (503), second connecting rod (504), first articulated shaft (505), second articulated shaft (506), third articulated shaft (507), third connecting rod (508), first slider (509), fourth articulated shaft (510), fifth articulated shaft (511), first temperature adjusting part (512), fourth connecting rod (513), fifth articulated shaft (514), fifth connecting rod (515), second slider (516), sixth articulated shaft (517), sixth connecting rod (518), seventh articulated shaft (519), second fixing part (520), eighth (521), linkage gear (522), rack (523), second temperature adjusting part (524), carriage (525), drive rod (526), linear motor (527), elastic roller (528), ninth articulated shaft (529), sleeve (530), fifth spring (531), A quill shaft (532), a tenth articulated shaft (533), a third temperature adjustment member (534);
the linear motor (527) is fixed on the upper surface of the base (201), the output end of the linear motor (527) is fixed at one end of the driving rod (526), the sliding frame (525) is in an n shape, and the other end of the driving rod (526) is fixed at the lower end of the sliding frame (525);
the first fixing piece (501) is fixed on the upper surface of the sliding frame (525), the second connecting rod (504) is in a 7 shape, one end of the second connecting rod (504) is connected with one end of the first connecting rod (502), and the bent part of the second connecting rod (504) is connected with the first fixing piece (501) through the second hinge shaft (506);
the connecting end of the first connecting rod (502) and the second connecting rod (504) is connected to one end of a fourth spring (503), the other end of the fourth spring (503) is fixed on the upper surface of the base (201), and the other end of the first connecting rod (502) is slidably connected with the top cover (3) through a first hinge shaft (505);
the other end of the second connecting rod (504) is connected with one end of a third connecting rod (508) and one end of a fourth connecting rod (513) through a third hinge shaft (507), the other end of the third connecting rod (508) is connected with the first sliding block (509) through a fourth hinge shaft (510), and the first sliding block (509) is connected with the first fixing piece (501) in a sliding manner;
the upper end of the first fixing piece (501) is connected with the first temperature adjusting piece (512) through the fifth hinge shaft (511), the first temperature adjusting piece (512) is provided with a protrusion, and the protrusion of the first temperature adjusting piece (512) is contacted with the top end of the first sliding block (509);
the other end of the fourth connecting rod (513) is connected with one end of the fifth connecting rod (515) and one end of a sixth connecting rod (518) through the fifth hinge shaft (514), the other end of the sixth connecting rod (518) is connected with the second fixing piece (520) through the seventh hinge shaft (519), and the second fixing piece (520) is fixed on the upper surface of the base (201);
the other end of the fifth connecting rod (515) is connected with the second sliding block (516) through the eighth hinge shaft (521), the second sliding block (516) is connected with the second fixing part (520) in a sliding manner, one side of the second sliding block (516) is provided with meshing teeth, the meshing teeth of the second sliding block (516) are meshed with the linkage gear (522), the rack (523) is connected with the second fixing part (520) in a sliding manner, and the rack (523) is meshed with the linkage gear (522);
the top end of the second fixing member (520) is connected with the second temperature adjusting member (524) through the sixth hinge shaft (517), the second temperature adjusting member (524) is provided with a protrusion, and the protrusion of the second temperature adjusting member (524) is contacted with the top end of the rack (523);
the outer edges of the first temperature regulating part (512) and the second temperature regulating part (524) are connected with the sleeve shaft (532) through the tenth hinged shaft (533), the fifth spring (531) is sleeved outside the sleeve shaft (532), the sleeve shaft (532) and the fifth spring (531) are inserted into the sleeve (530), and the sleeve (530) is connected with the first fixing part (501) and the second fixing part (520) through the ninth hinged shaft (529);
the third temperature adjusting part (534) is connected between the first fixing part (501) and the second fixing part (520);
heating pipes are arranged in the first temperature regulating part (512), the second temperature regulating part (524) and the third temperature regulating part (534);
the elastic rollers (528) are arranged on one sides, facing the incubator (209), of the first temperature adjusting part (512), the second temperature adjusting part (524) and the third temperature adjusting part (534).
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