CN206396237U - A kind of cell culture apparatus for simulating human body anaerobic environment - Google Patents
A kind of cell culture apparatus for simulating human body anaerobic environment Download PDFInfo
- Publication number
- CN206396237U CN206396237U CN201621492918.3U CN201621492918U CN206396237U CN 206396237 U CN206396237 U CN 206396237U CN 201621492918 U CN201621492918 U CN 201621492918U CN 206396237 U CN206396237 U CN 206396237U
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- China
- Prior art keywords
- room
- gas
- airway
- culturing room
- vertical clapboard
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Abstract
The utility model proposes a kind of cell culture apparatus for simulating human body anaerobic environment, including casing, the first air guide mechanism, the second air guide mechanism, box house is separated into first gas room, the first culturing room, the second culturing room, second gas room, first gas room and is provided with the first heating arrangements by the first vertical clapboard, the second vertical clapboard, the 3rd vertical clapboard;The second heating arrangements are provided with second gas room;The first airway and the second airway are provided with second vertical clapboard, second vertical clapboard is provided with multiple the first air inlet pipe connected with the first airway close to the first culturing room side, and the second vertical clapboard is provided with multiple the second air inlet pipe connected with the second airway close to the second culturing room side;First air guide mechanism is connected with first gas room, and the second air guide mechanism is connected with second gas room.The gas that the utility model can be used in the culture for having the cell of different requirements to anaerobic environment, the first culturing room, the second culturing room simultaneously is flowing, and carbon dioxide is evenly distributed.
Description
Technical field
The utility model is related to technical field of cell culture, more particularly to a kind of cell culture for simulating human body anaerobic environment
Device.
Background technology
CO2gas incubator is widely used in the culture of cell, tissue cultures and some peculiar microorganisms, is common in thin
Born of the same parents' dynamics research, the collection of mammalian cell secretion, various physics, carcinogenic or toxicological effect, the antigen of chemical factor
Research and production, culture hybridoma production antibody, inseminatio externalis (IVF), stem cell, organizational project, drug screening etc.
Research field.Gas in current CO2gas incubator is substantially immobilising so that carbon dioxide in whole incubator
Uneven concentration;And current CO2gas incubator is relatively simple, it is impossible to while carrying out having different requirements to anaerobic environment
Cell culture.
Utility model content
Based on technical problem present in background technology, the thin of human body anaerobic environment is simulated the utility model proposes a kind of
Born of the same parents' culture apparatus.
The utility model proposes a kind of simulation human body anaerobic environment cell culture apparatus, including casing, the first air guide
The first vertical clapboard, the second vertical clapboard, the 3rd vertical clapboard have been sequentially arranged in mechanism, the second air guide mechanism, casing in the horizontal direction,
Box house is separated into first gas room, the first culturing room, the second training by the first vertical clapboard, the second vertical clapboard, the 3rd vertical clapboard
Support and the first heating arrangements are provided with room, second gas room, first gas room, the first vertical clapboard is provided with multiple by first gas room
The first air inlet connected with the first culturing room;The second heating arrangements are provided with second gas room, the 3rd vertical clapboard is provided with more
Individual the second air inlet for connecting second gas room with the second culturing room;It is logical provided with the first airway and second in second vertical clapboard
Air flue, casing is provided with the first gas outlet connected with the first airway, the second gas outlet connected with the second airway, second
Vertical clapboard is provided with multiple the first air inlet pipe connected with the first airway close to the first culturing room side, and the second vertical clapboard is close to the
Two culturing room sides are provided with multiple the second air inlet pipe connected with the second airway;First air guide mechanism connects with first gas room
Connect, the second air guide mechanism is connected with second gas room.
It is preferred that, vertically arranged for interval has multiple first diaphragm plates in the first culturing room, and the first diaphragm plate is by
One culturing room is separated into the multiple first sub- culturing room.
It is preferred that, vertically arranged for interval has multiple second diaphragm plates in the second culturing room, and the second diaphragm plate is by
Two culturing room are separated into the multiple second sub- culturing room.
It is preferred that, the first air guide mechanism includes the first airway tube and the second airway tube, and the first airway tube is provided with the first valve
Door and its outlet side is connected with first gas room, the inlet end of the second airway tube is connected and its outlet side and the with the first gas outlet
One airway tube is connected, and the second airway tube is provided with the first air pump.
It is preferred that, the second air guide mechanism includes the 3rd airway tube and the 4th airway tube, and the 3rd airway tube is provided with the second valve
Door and its outlet side is connected with second gas room, the inlet end of the 4th airway tube is connected and its outlet side and the with the second gas outlet
Three airway tubes are connected, and the 4th airway tube is provided with the second air pump.
It is preferred that, the first air nozzle that gas is sprayed to the first culturing room is respectively equipped with the first air inlet.
It is preferred that, the second air nozzle that gas is sprayed to the second culturing room is respectively equipped with the second air inlet.
In the utility model, box house is separated into first gas by the first vertical clapboard, the second vertical clapboard, the 3rd vertical clapboard
It is provided with room, the first culturing room, the second culturing room, second gas room, first gas room on the first heating arrangements, the first vertical clapboard
Provided with multiple the first air inlets for connecting first gas room with the first culturing room;The second heater is provided with second gas room
Structure, the 3rd vertical clapboard is provided with multiple the second air inlets for connecting second gas room with the second culturing room;In second vertical clapboard
Provided with the first airway and the second airway, casing is provided with the first gas outlet connected with the first airway and the second ventilation
Road connection the second gas outlet, the second vertical clapboard close to the first culturing room side provided with it is multiple connected with the first airway first
Air inlet pipe, the second vertical clapboard is provided with multiple the second air inlet pipe connected with the second airway close to the second culturing room side;First
Air guide mechanism is connected with first gas room, and the second air guide mechanism is connected with second gas room.The utility model is by setting first
Culturing room, the second culturing room can be used for the cell culture for having different requirements to anaerobic environment, can be used for anaerobic environment
There is the culture of the cell of identical requirement;During for different cell culture, by the first air guide mechanism, the second air guide mechanism respectively to
First gas room, second gas room import various concentrations, the carbon dioxide of the confession cell culture of humidity;For same cell
During culture, by the first air guide mechanism, the second air guide mechanism respectively to first gas room, second gas room import same concentrations,
The carbon dioxide of the confession cell culture of humidity;Carbon dioxide adjusts temperature by the first heating arrangements, the second heating arrangements
The first culturing room, the second culturing room are respectively enterd after degree, and respectively outside the first airway, the second airway export casing with shape
Into flowing gas so that the first culturing room, the carbon dioxide of the second culturing room are evenly distributed, make the first culturing room, the second culture
Indoor all cells are in homogeneous good culture atmosphere.
Brief description of the drawings
Fig. 1 be the utility model proposes a kind of simulation human body anaerobic environment cell culture apparatus result schematic diagram.
Embodiment
Reference picture 1, the utility model proposes a kind of cell culture apparatus for simulating human body anaerobic environment, including casing 1, the
One air guide mechanism, the second air guide mechanism, wherein:
The first vertical clapboard 2, the second vertical clapboard 3, the 3rd vertical clapboard 4 are sequentially arranged in casing 1 in the horizontal direction, first erects
Dividing plate 2, the second vertical clapboard 3, the 3rd vertical clapboard 4 will be separated into first gas room 5, the first culturing room, the second culture inside casing 1
Room, second gas room 8.The first heating arrangements 9 for regulation of carbon dioxide gas temperature, first are provided with first gas room 5
Vertical clapboard 2 is provided with multiple the first air inlets for connecting first gas room 5 with the first culturing room, the first air inlet and set respectively
There is the first air nozzle 25 that gas is sprayed to the first culturing room.Vertically arranged for interval has multiple first in first culturing room
First culturing room is separated into multiple the first sub- culturing room 6 for being used to place Tissue Culture Flask by diaphragm plate 15, the first diaphragm plate 15.
Vertically arranged for interval has multiple second diaphragm plates 16 in second culturing room, and the second diaphragm plate 16 separates the second culturing room
Into multiple the second sub- culturing room 7 for being used to place Tissue Culture Flask.Being provided with second gas room 8 is used for regulation of carbon dioxide gas
Second heating arrangements 10 of temperature, the 3rd vertical clapboard 4 provided with it is multiple second gas room 8 is connected with the second culturing room second
The second air nozzle 26 that gas is sprayed to the second culturing room is respectively equipped with air inlet, the second air inlet.Set in second vertical clapboard 3
There are the first airway 11 and the second airway 12, casing 1 is provided with the first gas outlet and second connected with the first airway 11
The second gas outlet that airway 12 is connected, the second vertical clapboard 3 is close to the first culturing room side provided with multiple and the first airway 11
First air inlet pipe 13 of connection, the second vertical clapboard 3 is connected with the second airway 12 close to the second culturing room side provided with multiple
Second air inlet pipe 14.
First air guide mechanism is used to import the titanium dioxide used for the inner cell culture of the first culturing room into first gas room 5
Carbon gas, the first air guide mechanism includes the first airway tube 17 and the second airway tube 18, and the first airway tube 17 is provided with the first valve
19 and its outlet side connected with first gas room 5, the inlet end of the second airway tube 18 is connected and its outlet side with the first gas outlet
Connected with the first airway tube 17, the second airway tube 18 is provided with the first air pump 20.
Second air guide mechanism is used to import the titanium dioxide used for the inner cell culture of the second culturing room into second gas room 8
Carbon gas, the second air guide mechanism includes the 3rd airway tube 21 and the 4th airway tube 22, and the 3rd airway tube 21 is provided with the second valve
23 and its outlet side connected with second gas room 8, the inlet end of the 4th airway tube 22 is connected and its outlet side with the second gas outlet
Connected with the 3rd airway tube 21, the 4th airway tube 22 is provided with the second air pump 24.
The utility model proposes a kind of simulation human body anaerobic environment cell culture apparatus, pass through set first cultivate
Room, the second culturing room can be used for the cell culture for having different requirements to anaerobic environment, can be used for having phase to anaerobic environment
With the culture of desired cell;During for different cell culture, by the first airway tube 17, the 3rd airway tube 21 respectively to first
Gas compartment 5, second gas room 8 import various concentrations, the carbon dioxide of the confession cell culture of humidity;For same cell training
Support when, by the first airway tube 17, the 3rd airway tube 21 respectively to first gas room 5, second gas room 8 import same concentrations,
The carbon dioxide of the confession cell culture of humidity;Carbon dioxide is adjusted by the first heating arrangements 9, the second heating arrangements 10
The first sub- culturing room 6, the second sub- culturing room 7 are respectively enterd after temperature, and is entered respectively from the first airway 11, the second airway 12
Enter the second airway tube 18, in the 4th airway tube 22, then respectively enter first gas room 5, second gas room 8, so as to form flowing
Gas so that the first sub- culturing room 6, the carbon dioxide of the second sub- culturing room 7 are evenly distributed, makes the first sub- of culturing room 6, second
All cells in culturing room 7 are in homogeneous good culture atmosphere.
It is described above, only the utility model preferably embodiment, but protection domain of the present utility model is not
This is confined to, any one skilled in the art is in the technical scope that the utility model is disclosed, according to this practicality
New technical scheme and its utility model design are subject to equivalent substitution or change, should all cover in protection model of the present utility model
Within enclosing.
Claims (7)
1. it is a kind of simulate human body anaerobic environment cell culture apparatus, it is characterised in that including casing (1), the first air guide mechanism,
Second air guide mechanism, the first vertical clapboard (2), the second vertical clapboard (3) have been sequentially arranged in casing (1), the 3rd has been erected in the horizontal direction
Dividing plate (4), the first vertical clapboard (2), the second vertical clapboard (3), the 3rd vertical clapboard (4) will be separated into first gas inside casing (1)
The first heating arrangements (9) are provided with room (5), the first culturing room, the second culturing room, second gas room (8), first gas room (5),
First vertical clapboard (2) is provided with multiple the first air inlets for connecting first gas room (5) with the first culturing room;Second gas room
(8) the second heating arrangements (10) are provided with, the 3rd vertical clapboard (4) is provided with multiple by second gas room (8) and the second culturing room
Second air inlet of connection;It is provided with second vertical clapboard (3) on the first airway (11) and the second airway (12), casing (1)
The second gas outlet connected provided with the first gas outlet connected with the first airway (11), with the second airway (12), second erects
Dividing plate (3) is provided with multiple the first air inlet pipe (13) connected with the first airway (11) close to the first culturing room side, and second erects
Dividing plate (3) is provided with multiple the second air inlet pipe (14) connected with the second airway (12) close to the second culturing room side;First leads
Mechanism of qi structure is connected with first gas room (5), and the second air guide mechanism is connected with second gas room (8).
2. the cell culture apparatus of simulation human body anaerobic environment according to claim 1, it is characterised in that the first culturing room
Inside vertically arranged for interval has multiple first diaphragm plates (15), and the first culturing room is separated into multiple by the first diaphragm plate (15)
First sub- culturing room (6).
3. the cell culture apparatus of simulation human body anaerobic environment according to claim 1, it is characterised in that the second culturing room
Inside vertically arranged for interval has multiple second diaphragm plates (16), and the second culturing room is separated into multiple by the second diaphragm plate (16)
Second sub- culturing room (7).
4. the cell culture apparatus of the simulation human body anaerobic environment according to any one of claim 1-3, it is characterised in that
First air guide mechanism includes the first airway tube (17) and the second airway tube (18), and the first airway tube (17) is provided with the first valve
(19) and its outlet side is connected with first gas room (5), the inlet end of the second airway tube (18) is connected and it with the first gas outlet
Outlet side is connected with the first airway tube (17), and the second airway tube (18) is provided with the first air pump (20).
5. the cell culture apparatus of the simulation human body anaerobic environment according to any one of claim 1-3, it is characterised in that
Second air guide mechanism includes the 3rd airway tube (21) and the 4th airway tube (22), and the 3rd airway tube (21) is provided with the second valve
(23) and its outlet side is connected with second gas room (8), the inlet end of the 4th airway tube (22) is connected and it with the second gas outlet
Outlet side is connected with the 3rd airway tube (21), and the 4th airway tube (22) is provided with the second air pump (24).
6. the cell culture apparatus of the simulation human body anaerobic environment according to any one of claim 1-3, it is characterised in that
The first air nozzle (25) that gas is sprayed to the first culturing room is respectively equipped with first air inlet.
7. the cell culture apparatus of the simulation human body anaerobic environment according to any one of claim 1-3, it is characterised in that
The second air nozzle (26) that gas is sprayed to the second culturing room is respectively equipped with second air inlet.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201621492918.3U CN206396237U (en) | 2016-12-30 | 2016-12-30 | A kind of cell culture apparatus for simulating human body anaerobic environment |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201621492918.3U CN206396237U (en) | 2016-12-30 | 2016-12-30 | A kind of cell culture apparatus for simulating human body anaerobic environment |
Publications (1)
Publication Number | Publication Date |
---|---|
CN206396237U true CN206396237U (en) | 2017-08-11 |
Family
ID=59516182
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201621492918.3U Expired - Fee Related CN206396237U (en) | 2016-12-30 | 2016-12-30 | A kind of cell culture apparatus for simulating human body anaerobic environment |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN206396237U (en) |
-
2016
- 2016-12-30 CN CN201621492918.3U patent/CN206396237U/en not_active Expired - Fee Related
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Date | Code | Title | Description |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170811 Termination date: 20171230 |
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CF01 | Termination of patent right due to non-payment of annual fee |