CN205874455U - Indirect culture apparatus of cell - Google Patents
Indirect culture apparatus of cell Download PDFInfo
- Publication number
- CN205874455U CN205874455U CN201620834090.9U CN201620834090U CN205874455U CN 205874455 U CN205874455 U CN 205874455U CN 201620834090 U CN201620834090 U CN 201620834090U CN 205874455 U CN205874455 U CN 205874455U
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- cell
- culturing room
- room
- indirect
- culture device
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Abstract
The utility model discloses an indirect culture apparatus of cell, it includes first stock solution bottle, peristaltic pump, first cultivation room, second cultivation room and second stock solution bottle. The indoor millipore filtration that all is equipped with is cultivateed to first cultivation room and second, and millipore filtration's aperture can directly be passed through by the separation cell, and the vesica that can allow the emiocytosis is again secreted outward physically and soluble molecule passes through. The press handle type spring clamp is advantageous in tha, first cultivation room, the second is cultivateed the room and is separated the back by millipore filtration, four relatively independent regions have in other words, in these four relatively independent regions, same cultivation two indoor independent regional cells of placing the difference respectively, after the room is cultivateed from first cultivation room flow direction second to the culture solution, the body is secreted outward to the emiocytosis of first cultivation room, the factor is waited until the second and is cultivateed room direct action cultivateed the room in the second cell, form the automatics that the indirectness co -cultured, conveniently carry out the research of cell and cell interact relation. Simultaneously, work through the peristaltic pump to cultivate dynamic cultivation mode of indoor formation at first cultivation room, second, change the culture medium of two rooms at regular time and quantity, thereby can simulate internal body fluid endless internal environment.
Description
Technical field
This utility model belongs to medical experiment field, especially relates to a kind of indirect co-culture device of cell.
Background technology
Entity tumor not only comprises tumor cell and also comprises polytype mesenchyma stroma of tumors cell.Tumor cell and mesenchyma stroma of tumors
Interaction relationship between cell is that tumor occurs, is in progress and the emphasis of antitumor drug research.Tumor cell and interstitial are thin
Born of the same parents' each secrete cytokines, secrete body and other solable matters are mutually promoted outward, jointly promote tumour progression.But, how
Independent real-time study tumor cell and Interstitial cell interaction relationship are difficult problems.
The application being currently based on co-culture device provides ideal platform for tumor-Interstitial cell interaction relationship research.
Various based on the device co-cultured, it is exactly the most typically transwell Cell modeling, a kind of cell seeding is on cell
Layer, a kind of cell seeding is in cell lower floor, and centre is separated by the film of one layer of different pore size, and the surface of film can also spread one layer of base
Matter rubber moulding intends the structure of bio-matrix.But, this model is not best model, because it is a static culture pattern,
Static schema is unfavorable for the exchange of the culture supernatant of upper and lower two cells, the interior environment of simulation body-internal-circulation that can not be real.
Utility model content
The purpose of this utility model is to provide the indirect co-culture device of cell, it is possible to solve at least in the problems referred to above
Individual.
According to an aspect of the present utility model, it is provided that the indirect co-culture device of cell, it includes the first liquid containing bottle, compacted
Dynamic pump, the first culturing room, the second culturing room and the second liquid containing bottle.One end of peristaltic pump is with the first liquid containing bottle by entrance pipe even
Connecing, the other end of peristaltic pump is connected on the arrival end of the first connecting tube and the second connecting tube being set up in parallel, the first culturing room
Being arranged in the first connecting tube, the second culturing room is arranged in the second connecting tube；The first connecting tube being set up in parallel and second is even
The port of export of adapter is connected on the second liquid containing bottle by export pipeline after pooling together；First culturing room and the second culturing room
Inside being equipped with microporous filter membrane, the first culturing room and the second culturing room are all separated into two parts by microporous filter membrane, the first culturing room and
Being equipped with the culture medium cultivating cell in second culturing room, the aperture of microporous filter membrane can intercept cell and directly pass through, can permit again
Permitted to secrete body outside the vesicle of emiocytosis to pass through.
It provides the benefit that, after the first culturing room, the second culturing room are separated by microporous filter membrane, is equivalent to have four relatively
Independent region, in these four relatively independent regions, two isolated areas in same culturing room are placed different respectively
Cell, places tumor cell, places mesenchyma stroma of tumors cell for one for one, and the cell in Liang Ge culturing room can be placed, also in the same direction
Can be oppositely positioned, conveniently carry out lateral comparison or longitudinal comparison.When culture fluid flows to the second culturing room from the first culturing room
After, secrete body outside the emiocytosis of the first culturing room, the factor waits until that the second culturing room directly acts on the cell of the second culturing room, shape
Become the automaton of an Indirect co-culture, conveniently carry out the research of cell and cell interaction relation.Meanwhile, by wriggling
The work of pump, thus in the first culturing room, the second culturing room, form a dynamic training mode, change two at regular time and quantity
The culture medium of room, such that it is able to the interior environment of body fluid circulatory in analogue body.
Meanwhile, after microporous filter membrane is set, both can intercept tumor cell or mesenchyma stroma of tumors cell had directly passed through, the most mixed
Close；Can allow again to secrete body outside the vesicle of emiocytosis to pass through simultaneously, thus reach the effect of Indirect co-culture.
In the middle of some embodiments, the aperture of microporous filter membrane is 0.22 μm.It provides the benefit that, aperture is 0.22 μm
Time, just can intercept cell and pass through, but can allow to secrete body outside the vesicle of emiocytosis and pass through, such that it is able to simulation is true
Environment.
In the middle of some embodiments, the port of export of peristaltic pump is provided with Pressure gauge.It provides the benefit that, by arranging pressure
After power table, measurement that can be real-time, to the internal pressure situation of whole culture apparatus, prevents the situation that pressure is excessive, works as pressure
When power is excessive, the operating rate of peristaltic pump can be regulated accordingly, thus regulate the size of internal pressure.
In the middle of some embodiments, entrance pipe is provided with filter and check valve.It provides the benefit that, arranges filtration
Device is possible to prevent the impurity brought in the middle of culture medium to enter in the middle of the first culturing room and the second culturing room.
In the middle of some embodiments, entrance pipe is provided with oxygenator.It provides the benefit that, arranges oxygenator, permissible
The effective carbon dioxide removing the central generation of cyclic process, is converted into carbon dioxide oxygen simultaneously, supplies Liang Ge culturing room
Central cell, it is ensured that the normal growth of cell.
In the middle of some embodiments, the liquid of the first culturing room and the second culturing room flows to rear and is equipped with thief hole.
It provides the benefit that, after arranging thief hole, the corresponding liquid of convenient extraction is tested or detected.
In the middle of some embodiments, export pipeline is provided with pressure regulator valve.It provides the benefit that, by the tune of pressure regulator valve
Joint, can regulate the pressure size within whole culture apparatus, it is ensured that being smoothed out of incubation accordingly.
In the middle of some embodiments, the first culturing room and the second culturing room be on base in two ellipses trapping out recessed
Groove, the bottom of groove is provided with culture medium and imports and exports, and the top of groove is provided with top cover.It provides the benefit that, do ovalisation and not
Being square, be the dead angle in order to prevent culture medium from updating, some place square can produce eddy current, because the reason of eddy current cannot reach
To being that the culture medium supernatant (i.e. secreting body outside the vesicle of emiocytosis) in previous isolated area can update and flow to the next one
The purpose of isolated area.Culture medium is imported and exported can carry out adding or discharging the operation of culture medium easily.Top cover can be arranged
Become openable, by opening top cover, in that context it may be convenient to carry out the operations such as the bed board of cell, had digestive transfer culture.
In the middle of some embodiments, the first liquid containing bottle is additionally provided with charging aperture.It provides the benefit that, can facilitate to
Adding liquid culture medium in one liquid containing bottle.
Accompanying drawing explanation
Fig. 1 is the structure principle chart of the indirect co-culture device of cell of this utility model embodiment；
Fig. 2 is the structure principle chart of the first culturing room of the indirect co-culture device of cell of this utility model embodiment.
Detailed description of the invention
1 to 2 pair of this utility model is described in further detail below in conjunction with the accompanying drawings.
As it is shown in figure 1, the indirect co-culture device of the cell of embodiment of the present utility model, it include the first liquid containing bottle 1,
The 3, second culturing room of peristaltic pump the 2, first culturing room 4 and the second liquid containing bottle 5.One end of peristaltic pump 2 and the first liquid containing bottle 1 pass through into
Mouth pipeline 6 connects, and the other end of peristaltic pump 2 is connected to the first connecting tube 7 and the arrival end of the second connecting tube 8 being set up in parallel
On, the first culturing room 3 is arranged in the first connecting tube 7, and the second culturing room 4 is arranged in the second connecting tube 8；Be set up in parallel
The port of export of one connecting tube 7 and the second connecting tube 8 is connected on the second liquid containing bottle 5 by export pipeline 9 after pooling together；The
Being equipped with microporous filter membrane 10 in one culturing room 3 and the second culturing room 4, microporous filter membrane 10 is by the first culturing room 3 and the second culturing room 4
All it is separated into two parts, in the first culturing room 3 and the second culturing room 4, is equipped with the culture medium cultivating cell, microporous filter membrane 10
Aperture can intercept cell and directly pass through, and can allow again to secrete body outside the vesicle of emiocytosis and pass through.
After first the 3, second culturing room 4 of culturing room is separated by microporous filter membrane 10, be equivalent to that there are four relatively independent districts
Territory, in these four relatively independent regions, two isolated areas in same culturing room place different cells respectively, one
Placing tumor cell, place mesenchyma stroma of tumors cell for one, the cell in Liang Ge culturing room can be placed in the same direction, it is also possible to reversely puts
Put, conveniently carry out lateral comparison or longitudinal comparison.Meanwhile, by the work of peristaltic pump 2 so that in the middle of the first liquid containing bottle 1
Culture medium constantly flows to the second liquid containing bottle 5, in the middle of the process of motion, can pass through first the 3, second culturing room 4 of culturing room, from
And in first the 3, second culturing room 4 of culturing room, form a dynamic training mode, such that it is able to follow in real analogue body
The interior environment of ring.
Meanwhile, after microporous filter membrane 10 is set, both can intercept tumor cell or mesenchyma stroma of tumors cell had directly passed through, mutually
Mixing；Can allow again to secrete body outside the vesicle of emiocytosis to pass through simultaneously, thus reach the effect of simulation.
The aperture of the microporous filter membrane 10 in the middle of this utility model is 0.22 μm.When aperture is 0.22 μm, just can intercept
Cell passes through, but can allow to secrete body outside the vesicle of emiocytosis and pass through, such that it is able to simulate real environment.At peristaltic pump
The port of export of 2 is provided with Pressure gauge 11.After Pressure gauge 11 is set, can be real-time measure the inside of whole culture apparatus
Pressure condition, prevents the situation that pressure is excessive, when pressure is excessive, can regulate the operating rate of peristaltic pump 2 accordingly,
Thus regulate the size of internal pressure.
Meanwhile, entrance pipe 6 is provided with filter 12 and check valve 13.Arranging filter 12 is possible to prevent culture medium to work as
In the impurity brought into enter in the middle of the first culturing room 3 and the second culturing room 4.Entrance pipe 6 is provided with oxygenator 14.Arrange
Oxygenator 14, can effectively remove the carbon dioxide produced in the middle of cyclic process, carbon dioxide is converted into oxygen simultaneously, supplies
To the cell in the middle of Liang Ge culturing room, it is ensured that the normal growth of cell.
Liquid in the first culturing room 3 and the second culturing room 4 flows to rear and is equipped with thief hole 15.Thief hole 15 is set
After, the corresponding liquid of convenient extraction is tested or is detected.Export pipeline 9 is provided with pressure regulator valve 16.It provides the benefit that,
By the regulation of pressure regulator valve 16, the pressure size within whole culture apparatus can be regulated accordingly, it is ensured that incubation suitable
Profit is carried out.Meanwhile, the first liquid containing bottle 1 is additionally provided with charging aperture 20.Adding liquid in the first liquid containing bottle 1 can be facilitated to cultivate
Base.
In the middle of this utility model, the first culturing room 3 and the second culturing room 4 be on base in two ellipses trapping out recessed
Groove 17, as in figure 2 it is shown, the bottom of groove is provided with culture medium imports and exports 18, the top of groove is provided with top cover 19.Do ovalisation and
Not being square, be the dead angle in order to prevent culture medium from updating, some place square can produce eddy current, because the reason of eddy current cannot
Reach to be to secrete body outside the culture medium supernatant i.e. vesicle of emiocytosis in previous isolated area to update and flow to the next one
The purpose of isolated area.Culture medium imports and exports 18 can carry out adding or discharging the operation of culture medium easily.Top cover 19 is permissible
It is arranged to openable, by opening top cover 19, in that context it may be convenient to carry out the operations such as the bed board of cell, had digestive transfer culture.
Above is only preferred implementation of the present utility model, it is noted that for those of ordinary skill in the art
For, on the premise of creating design without departing from this utility model, it is also possible to make some deformation and improvement, these broadly fall into this
The protection domain of utility model.
Claims (9)
1. the indirect co-culture device of cell, it is characterised in that include the first liquid containing bottle (1), peristaltic pump (2), the first culturing room
(3), the second culturing room (4) and the second liquid containing bottle (5)；
One end of described peristaltic pump (2) is connected by entrance pipe (6) with described first liquid containing bottle (1), described peristaltic pump (2)
The other end is connected on the arrival end of the first connecting tube (7) and the second connecting tube (8) being set up in parallel, described first culturing room
(3) being arranged on described first connecting tube (7), described second culturing room (4) is arranged on described second connecting tube (8)；
The first connecting tube (7) being set up in parallel and the port of export of the second connecting tube (8) pass through export pipeline (9) after pooling together
It is connected on the second liquid containing bottle (5)；
Being equipped with microporous filter membrane (10) in described first culturing room (3) and the second culturing room (4), described microporous filter membrane (10) is by
One culturing room (3) and the second culturing room (4) are all separated into two parts, in described first culturing room (3) and the second culturing room (4) all
Being provided with the culture medium cultivating cell, the aperture of described microporous filter membrane (10) can intercept cell and directly pass through, can allow again cell
Secrete body outside the vesicle of secretion to pass through.
The indirect co-culture device of cell the most according to claim 1, it is characterised in that the aperture of described microporous filter membrane (10)
It it is 0.22 μm.
The indirect co-culture device of cell the most according to claim 1, it is characterised in that the port of export of described peristaltic pump (2)
It is provided with Pressure gauge (11).
The indirect co-culture device of cell the most according to claim 1, it is characterised in that described entrance pipe (6) is provided with
Filter (12) and check valve (13).
The indirect co-culture device of cell the most according to claim 1, it is characterised in that described entrance pipe (6) is provided with
Oxygenator (14).
The indirect co-culture device of cell the most according to claim 1, it is characterised in that described first culturing room (3) and
The liquid of two culturing room (4) flows to rear and is equipped with thief hole (15).
The indirect co-culture device of cell the most according to claim 1, it is characterised in that described export pipeline (9) is provided with
Pressure regulator valve (16).
The indirect co-culture device of cell the most according to claim 1, it is characterised in that described first culturing room (3) and institute
State the second culturing room (4) be on base in two oval-shaped grooves (17) trapping out, the bottom of described groove (17) is provided with cultivation
Base imports and exports (18), and the top of described groove (17) is provided with top cover (19).
The indirect co-culture device of cell the most according to claim 1, it is characterised in that described first liquid containing bottle (1) is upper also
It is provided with charging aperture (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620834090.9U CN205874455U (en) | 2016-08-03 | 2016-08-03 | Indirect culture apparatus of cell |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620834090.9U CN205874455U (en) | 2016-08-03 | 2016-08-03 | Indirect culture apparatus of cell |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205874455U true CN205874455U (en) | 2017-01-11 |
Family
ID=57702012
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620834090.9U Expired - Fee Related CN205874455U (en) | 2016-08-03 | 2016-08-03 | Indirect culture apparatus of cell |
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| CN (1) | CN205874455U (en) |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384719A (en) * | 2018-04-26 | 2018-08-10 | 中国烟草总公司郑州烟草研究院 | Cell co-culture device |
| CN108728360A (en) * | 2018-06-09 | 2018-11-02 | 上海天引生物科技有限公司 | A kind of devices and methods therefor that cell co-cultures |
| CN109337943A (en) * | 2018-11-01 | 2019-02-15 | 深圳市华唐兴东生物科技有限公司 | The method for producing tetraodotoxin |
| CN109439535A (en) * | 2018-12-04 | 2019-03-08 | 南昌大学 | A kind of micro algae culturing device |
| CN112410219A (en) * | 2020-12-04 | 2021-02-26 | 广东乾晖生物科技有限公司 | Cell culture system for continuous collection of exosomes |
| CN113817605A (en) * | 2021-09-18 | 2021-12-21 | 河南科技大学 | Dynamic cell culture device and single cell culture device |
| WO2022241633A1 (en) * | 2021-05-18 | 2022-11-24 | 广州赛莱拉干细胞科技股份有限公司 | Method for efficient preparation of exosomes using large-scale stem cell culture device |
-
2016
- 2016-08-03 CN CN201620834090.9U patent/CN205874455U/en not_active Expired - Fee Related
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384719A (en) * | 2018-04-26 | 2018-08-10 | 中国烟草总公司郑州烟草研究院 | Cell co-culture device |
| CN108384719B (en) * | 2018-04-26 | 2023-11-17 | 中国烟草总公司郑州烟草研究院 | cell co-culture device |
| CN108728360A (en) * | 2018-06-09 | 2018-11-02 | 上海天引生物科技有限公司 | A kind of devices and methods therefor that cell co-cultures |
| CN108728360B (en) * | 2018-06-09 | 2024-04-09 | 上海天引生物科技有限公司 | Cell co-culture device and method thereof |
| CN109337943A (en) * | 2018-11-01 | 2019-02-15 | 深圳市华唐兴东生物科技有限公司 | The method for producing tetraodotoxin |
| CN109337943B (en) * | 2018-11-01 | 2019-12-31 | 深圳市华唐兴东生物科技有限公司 | Method for producing tetrodotoxin |
| CN109439535A (en) * | 2018-12-04 | 2019-03-08 | 南昌大学 | A kind of micro algae culturing device |
| CN109439535B (en) * | 2018-12-04 | 2023-09-26 | 南昌大学 | Microalgae culture device |
| CN112410219A (en) * | 2020-12-04 | 2021-02-26 | 广东乾晖生物科技有限公司 | Cell culture system for continuous collection of exosomes |
| WO2022241633A1 (en) * | 2021-05-18 | 2022-11-24 | 广州赛莱拉干细胞科技股份有限公司 | Method for efficient preparation of exosomes using large-scale stem cell culture device |
| CN113817605A (en) * | 2021-09-18 | 2021-12-21 | 河南科技大学 | Dynamic cell culture device and single cell culture device |
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Legal Events
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| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20170111 Termination date: 20170803 |
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| CF01 | Termination of patent right due to non-payment of annual fee |