CN207130275U - A kind of co-culture device - Google Patents
A kind of co-culture device Download PDFInfo
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- CN207130275U CN207130275U CN201720851522.1U CN201720851522U CN207130275U CN 207130275 U CN207130275 U CN 207130275U CN 201720851522 U CN201720851522 U CN 201720851522U CN 207130275 U CN207130275 U CN 207130275U
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- culture
- capsule
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- rib
- culture device
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- 238000003501 co-culture Methods 0.000 title claims abstract description 29
- 239000002775 capsule Substances 0.000 claims abstract description 41
- 239000012528 membrane Substances 0.000 claims abstract description 24
- 238000009832 plasma treatment Methods 0.000 claims abstract description 6
- 150000001875 compounds Chemical class 0.000 claims abstract description 5
- 238000003475 lamination Methods 0.000 claims description 5
- 229920002799 BoPET Polymers 0.000 claims description 3
- 230000000737 periodic effect Effects 0.000 claims description 3
- 230000003993 interaction Effects 0.000 abstract description 4
- 239000002356 single layer Substances 0.000 abstract description 3
- 238000012364 cultivation method Methods 0.000 abstract description 2
- 230000008481 normal tissue growth Effects 0.000 abstract description 2
- 238000004088 simulation Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 31
- 238000002474 experimental method Methods 0.000 description 5
- 238000004113 cell culture Methods 0.000 description 4
- 238000012258 culturing Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 241001465754 Metazoa Species 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000001727 in vivo Methods 0.000 description 3
- 210000001519 tissue Anatomy 0.000 description 3
- 239000000969 carrier Substances 0.000 description 2
- 230000010261 cell growth Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 210000002683 foot Anatomy 0.000 description 2
- 238000000338 in vitro Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 210000002569 neuron Anatomy 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 240000001131 Nostoc commune Species 0.000 description 1
- 235000013817 Nostoc commune Nutrition 0.000 description 1
- 230000001464 adherent effect Effects 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 230000003915 cell function Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000008143 early embryonic development Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000004264 monolayer culture Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 210000004409 osteocyte Anatomy 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 210000003934 vacuole Anatomy 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a kind of co-culture device, including ware body, ware lid, support, capsule and culture membrane, the ware body top surface opening, the ware lid is covered on the opening of the ware body；The support is fastened in the ware body, is provided with the screens to match with the capsule the support array；The capsule is sticked in the screens, and capsule bottom surface opening and the culture membrane are compound in the opening of the capsule；The culture membrane is the culture membrane by plasma treatment.The co-culture device, by mediate contact, interaction between same environment culture various kinds of cell, cell, simulation and internal similar microenvironment, to solve the problems, such as that monolayer cultivation method can not provide normal tissue growth environment；To solve the problems, such as that culture environment is inconsistent, wastes time and energy.
Description
Technical field
It the utility model is related to a kind of cell culture apparatus, more particularly to a kind of co-culture device.
Background technology
The late 1980s, in order to establish the cultivating system for being more closely similar to vivo environment, make vitro as far as possible
Matched with vivo environment, the information so that iuntercellular can communicate with each other, mutually support growing multiplication, people are in cell culture skill
Cell Co culturing Techenique has been developed on the basis of art.Cell Co culturing Techenique is by 2 kinds or cell co-culture of more than two kinds
In same environment, because it has the advantages of preferably reflecting vivo environment, it is widely used in the modern times in this way
In cell research.
At present, Cell Co culturing Techenique is at most applied to osteocyte and nerve cell.Cell co-culture system mainly passes through
Two methods are established：1) Co-culture system, i.e., 2 kinds or cell of more than two kinds are inoculated in same hole at the same time or separately,
Directly contacted between different types of cell；2) Indirect co-culture system, i.e., 2 kinds or cell of more than two kinds are inoculated in respectively
On different carriers, among both carriers then are placed in into same culture environment, share different types of cell same
Cultivating system and be not directly contacted with.Co-culture system main function：Inducing cell is to another cell differentiation；Inducing cell itself
Differentiation；Maintain cell function and vigor；Regulating cell is bred；Promote early embryonic development and improve metabolite yield.
At present the following aspects is mainly reflected in the defects of technology with deficiency：
1) conventional cultured in monolayer in vitro method can not provide the environmental condition needed for tissue normal growth and development, every in vivo
The genome of individual cell is identical, but its phenotype is different with functional structure in different organ and tissue, under many circumstances, individual layer
Cell culture can gradually lose original biological characteristics because of the change of vitro, and extraterrestial process occur in internal effect
Entirely different experimental result.
2) various kinds of cell is included in histoorgan, when will spend substantial amounts of such as the interaction between observation two-by-two cell
Between, energy and expense, and need the culture of multiple various kinds of cell, condition of culture has differences.
3) at present, experiment in vivo is more based on zoopery, but Integral animal experiment is time-consuming long, experimental result is unstable,
Repeatability is relatively low, letting animals feed expends great effort and financial resources, the detection and observation of experimentation is not easy to, by experimental animal number
The limitation of amount and species, zoopery are difficult to react sensitiveness of the real clinical patient to medicine, at the same zoopery against
It is humanistic.
Utility model content
For overcome the deficiencies in the prior art, the purpose of this utility model is to provide a kind of non-direct contact type, culture
Environment identical co-culture device.
The purpose of this utility model adopts the following technical scheme that realization：
A kind of co-culture device, including ware body, ware lid, support, capsule and culture membrane, ware body top surface opening, ware lid cover
In the opening of ware body；Support is fastened in ware body, is provided with the screens to match with capsule support array；Capsule is sticked in card
In position, capsule bottom surface opening and culture membrane are compound in the opening of capsule；Culture membrane is the culture membrane by plasma treatment.
Further, the outer wall of capsule is provided with by pushing up along the rib extended downwardly.
Further, the bar number of the rib is more than 2, and is circumferentially uniformly distributed.
Further, rib includes long rib, middle rib and short rib；Long rib, middle rib and short rib circumferentially periodic distribution successively.
Further, capsule side wall lower edge is down along some circumferential arc-shaped feets of stretching.
Further, culture membrane is PET film or PC films.
Further, support is provided with the leg extended downwardly.
Further, outer of the support along screens is provided with the support block upwardly extended.
Further, screens rectangular array is distributed, and support is flatly provided with arc spring lamination on four angles.
Further, the external wall of ware is provided with spacing edge.
Compared with prior art, the beneficial effects of the utility model are：
1st, the utility model design Cell culture invitro mode, while two or more cell is inoculated in respectively respectively
In individual co-cultivation capsule, between same environment culture various kinds of cell, cell mediate contact, interaction, increase cell between
Information interchange, by Cell Co culturing Techenique, simulation and similar microenvironment in vivo, are the offer such as downstream drug functional study
Relatively reliable experimental basis and technical support；Asking for normal tissue growth environment can not be provided to solve monolayer cultivation method
Topic；
2nd, co-culture device provided by the utility model, by introducing support in Nostoc commune Vanch ware, array in support
Ground is equipped with a co-cultivation capsule, while with environment culture Various Tissues cell, by once testing i.e. observable cell two-by-two
Or the interaction between various kinds of cell, repeated multiple times experiment is avoided, greatly reducing manpower, financial resources, the consumption of time,
To solve the problems, such as that culture environment is inconsistent, wastes time and energy；
3rd, co-culture device provided by the utility model provides the co-cultivation environment of two kinds or more of cells simultaneously, in vitro
Cell experiment can directly observe living cells morphosis and vital movement, directly the change of observation cell, be easy to provide it is a large amount of
The similar experimental subjects of biological character and cost are time-consuming few, and co-culture environment and can provide cell and mutually exchange, mutually support
The environment of propagation, relatively it is coincide with vivo environment, has filled up the wide gap of monolayer cell culture and Integral animal experiment, while meet people
Road doctrine requirement.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model；
Fig. 2 is fractionation structural representation of the present utility model；
Fig. 3 is the structural representation of capsule；
Fig. 4 is the structural representation of support.
In figure, each reference：1st, ware body；11st, spacing edge；12nd, Handheld Division；2nd, ware lid；3rd, support；31st, screens；32、
Leg；33rd, support block；34th, arc spring lamination；4th, capsule；41st, rib；411st, long rib；412nd, middle rib；413rd, short rib；414th, arc
Pin；5th, culture membrane.
Embodiment
Below, with reference to accompanying drawing and embodiment, the utility model is described further, it is necessary to explanation is,
On the premise of not colliding, it can be formed in any combination between various embodiments described below or between each technical characteristic new
Embodiment.
In detailed description below, such as non-specified otherwise, described " top ", " bottom " etc. have temporald eixis implication
Word, it is understood that to the temporald eixis of accompanying drawing.
The utility model provides a kind of co-culture device, as Figure 1-4, including ware body 1, ware lid 2, support 3, capsule 4
With culture membrane 5, the top surface opening of ware body 1, ware lid 2 is covered on the opening of ware body 1；Support 3 is fastened in ware body 1, the array of support 3
Ground is provided with the screens 31 to match with capsule 4；Capsule 4 is sticked in screens 31, the bottom surface opening of capsule 4 and culture membrane 5 is compound in
The opening of capsule 4, culture membrane 5 are the culture membrane 5 by plasma treatment.
Co-culture device provided by the utility model, multiple relatively independent culture capsules 4, membranelle are supported through support 3
Bottom is provided with culture membrane 5 Jing Guo plasma treatment, can provide good cell growth environment, be advantageous to the growth of cell with
Propagation, is advantageous to the more difficult adherent cell such as nerve cell and realizes good adhere-wall culture.The co-cultivation capsule 4 is in support
3 and culture dish support the use jointly, be easily assembled, there is preferable flexibility, it is easily operated.
In this co-culture device, capsule includes but is not limited to chimeric or bonding, i.e. culture membrane with the complex method of culture membrane
The opening of capsule is compound in a manner of air-tightness.
Co-culture device provided by the utility model, the connectivity of capsule 4 and support 3 can be improved by the following means,
Such as：1) outer wall of capsule 4 is provided with by pushing up along the rib 41 extended downwardly, the interference card of screens 31 that capsule 4 passes through the support 3 of rib 41
Connect；2) outer of the support 3 along screens 31 is provided with the support block 33 upwardly extended.Can also further, support block 33 is as limit
Bit architecture, the bottom of rib 41 offset with the top bottom of support block 33, so that each capsule 4 is caught in the depth one of screens 31
Cause.
On the basis of the above, can further, the quantity of rib 41 is at least three, is circumferentially uniformly distributed or along axle
Be centrosymmetric distribution；Or yet further, rib 41 includes long rib 411, middle rib 412 and short rib 413；Long rib 411, middle rib
412 and the circumferentially periodic distribution successively of short rib 413；By rotating capsule 4, make long rib 411 or middle rib 412 or short rib 413
Bottom and the top of support block 33 offset, to control capsule 4 to stretch into the depth of screens 31.
Can also further, in order to avoid the nutrient solution in culture membrane 5 and ware body 1 forms bubble liquid in the utility model
Film and cause Culture liquid measure in capsule 4 different, the side wall lower edge of capsule 4 is down along stretching out some circumferential arc-shaped feets
414。
Culture membrane 5 in the utility model is the PET film or PC films by plasma treatment.
Support 3 of the present utility model can further be provided with the leg 32 extended downwardly, on the one hand improve the flat of support 3
Stability, it on the other hand can also effectively avoid forming vacuole between nutrient solution and culture membrane 5.Or further, support 3 is along screens
31 outer is provided with the support block 33 upwardly extended, and support block 33 as above, can engage or by rib 41 with the interference of capsule 4
Lower edge it is spacing.Or further, the rectangular array of screens 31 distribution, support 3 is flatly provided with arc spring lamination on four angles
34.The inwall of arc spring lamination 34 and ware body 1 on four angles offsets, so that support 3 blocks flatly together in ware body 1.Support 3
Material be preferably hard elastic plastic material, such as PS.
The utility model can also further, in order to facilitate the connectivity of ware body 1 and ware body 1 and ware lid 2, ware body 1
Outer wall is provided with spacing edge 11.Or further, ware body 1 is gripped for convenience, spacing edge 11 is additionally provided with circumferentially equally distributed
Some Handheld Divisions 12.
Above-mentioned embodiment is only preferred embodiment of the present utility model, it is impossible to is protected with this to limit the utility model
Scope, the change of any unsubstantiality that those skilled in the art is done on the basis of the utility model and replacing belongs to
In the utility model scope claimed.
Claims (10)
- A kind of 1. co-culture device, it is characterised in that including ware body, ware lid, support, capsule and culture membrane, the ware body top surface Opening, the ware lid are covered on the opening of the ware body；The support is fastened in the ware body, is set the support array There is the screens to match with the capsule；The capsule is sticked in the screens, capsule bottom surface opening and the culture Film is compound in the opening of the capsule；The culture membrane is the culture membrane by plasma treatment.
- 2. co-culture device as claimed in claim 1, it is characterised in that the outer wall of the capsule is provided with to be extended downwardly by top edge Rib.
- 3. co-culture device as claimed in claim 2, it is characterised in that the bar number of the rib is more than 2, and circumferentially equal Even distribution.
- 4. co-culture device as claimed in claim 3, it is characterised in that the rib includes long rib, middle rib and short rib；It is long Rib, middle rib and short rib circumferentially periodic distribution successively.
- 5. co-culture device as claimed in claim 1, it is characterised in that the capsule side wall lower edge is down along some edges of stretching The arc-shaped feet of circle distribution.
- 6. co-culture device as claimed in claim 1, it is characterised in that the culture membrane is PET film or PC films.
- 7. co-culture device as claimed in claim 1, it is characterised in that the support is provided with the leg extended downwardly.
- 8. co-culture device as claimed in claim 1, it is characterised in that outer of the support along the screens is provided with upward The support block of extension.
- 9. co-culture device as claimed in claim 1, it is characterised in that the screens rectangular array distribution, the support Arc spring lamination is flatly provided with four angles.
- 10. co-culture device as claimed in claim 1, it is characterised in that the external wall of ware is provided with spacing edge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720851522.1U CN207130275U (en) | 2017-07-13 | 2017-07-13 | A kind of co-culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720851522.1U CN207130275U (en) | 2017-07-13 | 2017-07-13 | A kind of co-culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN207130275U true CN207130275U (en) | 2018-03-23 |
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| CN201720851522.1U Active CN207130275U (en) | 2017-07-13 | 2017-07-13 | A kind of co-culture device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384719A (en) * | 2018-04-26 | 2018-08-10 | 中国烟草总公司郑州烟草研究院 | Cell co-culture device |
-
2017
- 2017-07-13 CN CN201720851522.1U patent/CN207130275U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108384719A (en) * | 2018-04-26 | 2018-08-10 | 中国烟草总公司郑州烟草研究院 | Cell co-culture device |
| CN108384719B (en) * | 2018-04-26 | 2023-11-17 | 中国烟草总公司郑州烟草研究院 | cell co-culture device |
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