CN202181306U - Various-cell cocultivation support and various-cell cocultivation device - Google Patents
Various-cell cocultivation support and various-cell cocultivation device Download PDFInfo
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- CN202181306U CN202181306U CN2011203078457U CN201120307845U CN202181306U CN 202181306 U CN202181306 U CN 202181306U CN 2011203078457 U CN2011203078457 U CN 2011203078457U CN 201120307845 U CN201120307845 U CN 201120307845U CN 202181306 U CN202181306 U CN 202181306U
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Abstract
The utility model relates to a various-cell cocultivation support, which comprises a support surface and a supporting frame connected with the support surface. Fixing holes are disposed on the support surface. The supporting frame is connected to edges of the support surface. Cocultivation of three to five types of cells can be carried out according to needs, the various-cell cocultivation support is applicable to cocultivation of various attached cells, suspension or semi-suspension cells, various cells respectively grow in different small culture rooms or culture vessels, pollution or mixing of different cells can be avoided, and cells are easy to obtain.
Description
Technical field
The utility model relates to a kind of cultivation support and culture apparatus, particularly relates to one kind of multiple co-culture of cells support and culture apparatuses.
Background technology
Cell cultures is cell to be placed grow under the vitro conditions and breed, but have the morphological structure of direct viewing viable cell and vital movement, direct viewing cell variation, be easy to provide similar experimental subjects of large number of biological proterties and expensive advantage such as few.It can be a kind of cell single culture and various kinds of cell co-cultivation that cells in vitro is cultivated.
A kind of single culture of cell; This is at present in preclinical medicine and basis and the clinical research that combines, to use mode the most widely, is mainly used in the variation and the mechanism thereof of morphology, gene and proteic expression and the generation under the effect of different treatment factor of a certain cell of research.Though cell is independently survived in manual simulation's internal milieu in the culturing process, this environment is compared with real internal milieu and is still had very big-difference.In order to set up the culture system that more is similar to internal milieu; External environment and internal milieu are matched; Thereby make the iuntercellular information of communicating with each other; Each other support growing multiplication, in the later stage eighties 20th century, people are developed on the basis of original cell cultures and the co-culture of cells technology.The co-culture of cells technology is with the technology of cell co-cultivation in same environment more than 2 kinds or 2 kinds, because it has the advantage of better reflection internal milieu, so this method is applied in the modern cell research more and more widely.
The various kinds of cell co-cultivation is mainly set up through two kinds of methods: directly co-culture system and co-culture system indirectly.Direct co-culture system; Be that the cell more than 2 kinds or 2 kinds is inoculated in the same culture vessel simultaneously, directly contact is undeniable between the dissimilar cells; More a kind of cell single culture of this mode can better reflect intravital environment; But because different cells is grown in the same cell container, can't separates various cells and be used for next step research, limit its application.Indirect co-culture system; The cell that is about to more than 2 kinds or 2 kinds is inoculated in respectively on the different carriers; Then different carriers is placed same culture environment; Different types of cell is co-located in in a kind of culture system and not directly contact, can collects different types of cell respectively and be used for next step research, remedied the deficiency of direct co-culture system.At present commercial is the system that can be used for 2 kinds of co-culture of cells; The co-culture system (claiming the transwell petridish again) that commercial all size is arranged like U.S. CORNING (corning) company; U.S. millipore company then produces applicable to domestic and international each family and cultivates suspension type cell of vessel production company; Assembling becomes co-culture system, and these commercial co-culture systems are simple to operate, are used widely under study for action at present.But; Because in the incidence and development process of various disease; The cell type of participating possibly be many kinds; And complex interactions is the major cause that disease produces between the various kinds of cell, and the investigator presses for and can many cells more be brought into in a kind of culture environment both at home and abroad, and the co-culture system that can further study various cell types respectively.In addition, for adapting to the demand that new chemicals or product increase fast, press for quick, the high-throughout toxicity assessment method of development.In vitro method cultured cells kind commonly used is limited; The investigator need estimate susceptibility and the toxic action of poisonous substance to multiple organ cell simultaneously; For satisfying this requirement; Six orifice plates of usefulness Corning Incorporateds such as the Li AP of the U.S. improve, and can cultivate 6 kinds of cells from Different Organs simultaneously, are called integrated discrete multiple organ co-culture (IdMOC
TM) plate, Chinese is whole many organs of discontinuity co-culture system, this product patent applied for (US 7186548B2, CHINA 626030).But not enough below this product exists: 1) big being total in the culture hole at culture plate has 6 apertures; The height of each aperture has only 0.8 millimeter; And distance is too near between each aperture; Therefore if slight rocking arranged when operation, the cell in aperture may move in another aperture, cause between the different cells pollution with mix; And, can cause cell attachment inhomogeneous owing to can not rock, and it is many centrocyte to occur, and the situation that peripheral cell is few influences the growth of cell; And operation also has certain degree of difficulty when harvested cell; 2) because the growth area limited (liquid that can only hold 300 microlitres in each aperture at most) of aperture needing to be not suitable for the experiment of a large amount of cells; 3) only be fit to attached cell, be not suitable for suspending and the cultivation of half suspension cell.
In sum, but the present system IdMOC of existing various kinds of cell co-cultivation
TMFollowing deficiency is arranged: 1) be prone to cause between the different cells pollution with mix; 2) cell attachment is inhomogeneous; 3) be not suitable for needing the experiment of a large amount of cells; 4) only be fit to attached cell, be not suitable for suspending and the cultivation of half suspension cell; 5) harvested cell acquires a certain degree of difficulty.In addition, IdMOC
TMSystem is mainly used in estimates susceptibility and the toxic action of poisonous substance to multiple organ cell, is difficult to satisfy the different demands that numerous investigators cultivate for various kinds of cell altogether.For example; In the generating process of hepatic fibrosis, there are three kinds of cells bringing into play crucial effect jointly: hepatocellular injury, necrosis; Stimulate and activation liver scavenger cell (Kupffer cell); Latter's activation secrete cytokines activates the liver stellate cell again and produces excessive extracellular matrix, and the excessive accumulation of extracellular matrix then causes the generation of hepatic fibrosis; In addition, adhesion molecule also significantly raises in liver fibrosis process, its raise then with liver cell and blood sinus endotheliocyte abduction delivering Integrin family in the laminin acceptor relevant.In the pathogenesis of other diseases, also often there is the interaction of (often being 3 to 5 kinds) key cells more than 3 kinds or 3 kinds to participate, and has various kinds of cell to belong to suspension or half suspension growth cell.Therefore, design easy handling, be applicable to that attached cell and suspension cell co-cultivation, the culture system that is applicable to the multiple different needs of investigator are very necessary simultaneously.
Summary of the invention
For avoiding the deficiency of above prior art, the utility model provides one kind of multiple co-culture of cells support and culture apparatuses, to solve a culture system that can carry out the various kinds of cell co-cultivation.
The technical scheme of the utility model is:
Various kinds of cell is cultivated support altogether, and this support comprises stent cover and the bracing frame that is connected with said stent cover, offers fixed orifices on the described stent cover.
Described stent cover edge is connected with bracing frame.
The edge of described fixed orifices is provided with open slot.
Described fixed orifices is at least 2.
Described open slot is 3.
The height of described bracing frame is for being 17-20mm.
Various kinds of cell co-culture device, this device comprise that petridish and the many cells that are positioned in the petridish cultivate support altogether, and these many cells are cultivated support altogether and comprised stent cover and the bracing frame that is connected with said stent cover, offer fixed orifices on the described stent cover.
The edge of the fixed orifices of this device is provided with open slot.
This device also includes the cultivation cell.
Described many cells are cultivated the height of the height of support greater than said cultivation cell altogether, and less than the height of described petridish.
When carrying out the various kinds of cell co-cultivation; The cultivation cell is put into many cells cultivates altogether on the fixed orifices on the support, cultivate the height of the height of cell, under the support of cultivating support less than petridish; Maintain certain space between the bottom of cultivation cell and the bottom of petridish container; When each is cultivated cell in the cell and can be in independent culture environment, can place a big culture system again, and various cell can not contact directly again each other.
On the other hand, when carrying out the many cells cultivation, when rocking operation to cultivating cell; Have stationary installation owing to cultivate cell; This stationary installation is connected with the open slot coupling of fixed orifices, and the position of cultivation cell can be easy to be fixed and be unlikely to and slide, to influence experimental result.
The utlity model has following advantage:
1) can carry out the co-cultivation of 3 to 5 kinds of cells as required;
2) applicable to the co-cultivation of multiple attached cell;
3) be applicable to the co-cultivation of multiple suspension or half suspension cell;
4) be applicable to the co-cultivation of attached cell and suspension or half suspension cell;
5) various cells grow in respectively in different culture cell or the petridish, can not cause between the different cells pollution with mix, be easy to harvested cell;
6) can make cell attachment even through slight shaking culture cell mixing cell.
Description of drawings
Fig. 1 (a): the support front plan view is cultivated in 3 holes altogether;
Fig. 1 (b): the support stereographic map is cultivated in 3 holes altogether;
Fig. 2 (a): the support front plan view is cultivated in 3 holes of band open slot altogether;
Fig. 2 (b): the support stereographic map is cultivated in 3 holes of band open slot altogether;
Fig. 3: the petridish front view in the many cells co-culture device.
Embodiment
Below in conjunction with accompanying drawing the utility model is done further detailed description.
Cultivating support altogether with 3 holes is example to the common cultivation of 3 kinds of cells:
Shown in Fig. 1 (a), Fig. 1 (b); One kind of multiple co-culture of cells supports; This support comprises stent cover 1 and the bracing frame 2 that is connected with said stent cover 1, offers fixed orifices 3 on the described stent cover 1, and described stent cover 1 can be located to be connected with described bracing frame 2 on the edge of.Described fixed orifices is 3.The height of described bracing frame is for selecting 17mm.
Be illustrated in figure 3 as the petridish front view in the many cells co-culture device.One kind of multiple cell co-culture devices, this device comprise petridish 4 and are positioned over the common cultivation support in the petridish that this is cultivated support altogether and comprises stent cover and the bracing frame that is connected with said stent cover, offers fixed orifices on the described stent cover.This device also includes the cultivation cell.Described cultivation cell is a capsule, and the edge of capsule is provided with the stationary installation of mating with said open slot.
Above-mentioned petridish can adopt 10 centimetres of U.S. corning company diameters, highly is the petridish of 2 centimetres or equal specification; Cultivating cell adopts millipore company suspension type to cultivate cell.Because the utility model is cultivated the height of the bracing frame of support and is designed to 17mm, therefore will be loaded with the cultivation support of cultivating cell and put into petridish, certain distance is left with the petridish bottom in this cultivation cell bottom, can directly not contact each other.Described petridish, cultivate support and suspension type altogether and cultivate cell and then become a co-culture system that can carry out the various kinds of cell co-cultivation, each suspension type is cultivated in cell can add different types of cell (can be attached cell or suspension cell) respectively.Below 3 kinds of cell co-cultivation are described.
At first; With 3 kinds of different types of cells (can be attached cell or suspension cell); Respectively put into the cultivation cell of common cultivation support like liver cell, scavenger cell and stellate cell; Again this support is put into petridish, place cell culture incubator to cultivate for some time (6 hours or incubated overnight) whole co-culture system, make attached cell fully adherent (the growth characteristics difference of every kind of cell causes the adherent time that certain difference is arranged).Cultivating the liquid that can add 2 to 3 milliliters in the cell, is 1 * 10 with density
7The cell of/milliliter is an example, can add 2 to 3 * 10
7Individual cell needing to be suitable for the experiment of a large amount of cells.Respectively cultivate between the cell cell this moment and independently grow, nutrient solution does not interconnect yet.
Secondly, treat that attached cell is adherent after, remove unnecessary nutrient solution, in petridish, add fresh medium, highly make it can not have suspension type to cultivate the bottom of cell.Because suspension type is cultivated the aperture that different sizes are arranged at the bottom of cell, can allow liquid freely to pass through, and cell can not pass through, therefore, 3 kinds of cells can interconnect through nutrient solution, are in the same growing environment.Be defined as the time that 3 kinds of co-culture of cells begin this moment, according to different investigators' demand, in nutrient solution, adds exogenous fiddle factors.
Experiment according to investigator's requirement, can be collected the supernatant of co-culture system after finishing, and observes the variation of secreted protein in the co-culture system; Different suspension type petridish is taken out, collect 3 kinds of different cells respectively, be used for next step experiment.
Cultivating support altogether with 3 holes is example to the common cultivation of 3 kinds of cells:
Shown in Fig. 2 (a), Fig. 2 (b); One kind of multiple co-culture of cells supports; This support comprises stent cover 1 and the bracing frame 2 that is connected with said stent cover 1, offers fixed orifices 3 on the described stent cover 1, and described stent cover 1 can be located to be connected with described bracing frame 2 on the edge of.The edge of described fixed orifices 3 is provided with open slot 31.Described fixed orifices 3 is 3.Described open slot 31 is 3.The height of described bracing frame is for being 18mm.
As shown in Figure 3, one kind of multiple cell co-culture devices, this device comprise petridish 4 and are positioned over the common cultivation support in the petridish that this is cultivated support altogether and comprises stent cover and the bracing frame that is connected with said stent cover, offers fixed orifices on the described stent cover.The edge of described fixed orifices is provided with open slot.This device also includes the cultivation cell.Described cultivation cell is a capsule, and the edge of capsule is provided with the stationary installation of mating with said open slot.
Above-mentioned petridish can adopt 10 centimetres of U.S. corning company diameters, highly is the petridish of 2 centimetres or equal specification; Cultivating cell adopts millipore company suspension type to cultivate cell.Because the utility model is cultivated the height of the bracing frame of support and is designed to 18mm, therefore will be loaded with the cultivation support of cultivating cell and put into petridish, certain distance is left with the petridish bottom in this cultivation cell bottom, can directly not contact each other.Described petridish, cultivate support and suspension type altogether and cultivate cell and then become a co-culture system that can carry out the various kinds of cell co-cultivation, each suspension type is cultivated in cell can add different types of cell (can be attached cell or suspension cell) respectively.Below 3 kinds of cell co-cultivation are described.
At first; With 3 kinds of different types of cells (can be attached cell or suspension cell); Respectively put into the cultivation cell of common cultivation support like liver cell, scavenger cell and stellate cell; Again this support is put into petridish, place cell culture incubator to cultivate for some time (6 hours or incubated overnight) whole co-culture system, make attached cell fully adherent (the growth characteristics difference of every kind of cell causes the adherent time that certain difference is arranged).Cultivating the liquid that can add 2 to 3 milliliters in the cell, is 1 * 10 with density
7The cell of/milliliter is an example, can add 2 to 3 * 10
7Individual cell needing to be suitable for the experiment of a large amount of cells.Respectively cultivate between the cell cell this moment and independently grow, nutrient solution does not interconnect yet.
Secondly, treat that attached cell is adherent after, remove unnecessary nutrient solution, in petridish, add fresh medium, highly make it can not have suspension type to cultivate the bottom of cell.Because suspension type is cultivated the aperture that different sizes are arranged at the bottom of cell, can allow liquid freely to pass through, and cell can not pass through, therefore, 3 kinds of cells can interconnect through nutrient solution, are in the same growing environment.Be defined as the time that 3 kinds of co-culture of cells begin this moment, according to different investigators' demand, in nutrient solution, adds exogenous fiddle factors.
Experiment according to investigator's requirement, can be collected the supernatant of co-culture system after finishing, and observes the variation of secreted protein in the co-culture system; Different suspension type petridish is taken out, collect 3 kinds of different cells respectively, be used for next step experiment.
Cultivating support altogether with 3 holes is example to the common cultivation of 4 kinds of cells:
Shown in Fig. 1 (a), Fig. 1 (b); One kind of multiple co-culture of cells supports; This support comprises stent cover 1 and the bracing frame 2 that is connected with said stent cover 1, offers fixed orifices 3 on the described stent cover 1, and described stent cover 1 can be located to be connected with described bracing frame 2 on the edge of.Described fixed orifices is 3.The height of described bracing frame 2 is for being 19mm.
As shown in Figure 3, one kind of multiple cell co-culture devices, this device comprise petridish 4 and are positioned over the common cultivation support in the petridish that this is cultivated support altogether and comprises stent cover and the bracing frame that is connected with said stent cover, offers fixed orifices on the described stent cover.This device also includes the cultivation cell, and described cultivation cell is a capsule, and the edge of capsule is provided with the stationary installation of mating with said open slot.
Above-mentioned petridish can adopt 10 centimetres of U.S. corning company diameters, highly is the petridish of 2 centimetres or equal specification; Cultivating cell adopts millipore company suspension type to cultivate cell.Because the utility model is cultivated the height of the bracing frame of support and is designed to 19mm, therefore will be loaded with the cultivation support of cultivating cell and put into petridish, certain distance is left with the petridish bottom in this cultivation cell bottom, can directly not contact each other.Described petridish, cultivate support and suspension type altogether and cultivate cell and then become a co-culture system that can carry out the various kinds of cell co-cultivation, each suspension type is cultivated in cell can add different types of cell (can be attached cell or suspension cell) respectively.Below 4 kinds of cell co-cultivation are described.
At first; With 3 kinds of different types of cells (can be attached cell or suspension cell); Respectively put into the cultivation cell of common cultivation support like liver cell, scavenger cell and stellate cell; Again this support is put into petridish, place cell culture incubator to cultivate for some time (6 hours or incubated overnight) whole co-culture system, make attached cell fully adherent (the growth characteristics difference of every kind of cell causes the adherent time that certain difference is arranged).Cultivating the liquid that can add 2 to 3 milliliters in the cell, is 1 * 10 with density
7The cell of/milliliter is an example, can add 2 to 3 * 10
7Individual cell needing to be suitable for the experiment of a large amount of cells.Respectively cultivate between the cell cell this moment and independently grow, nutrient solution does not interconnect yet.
Secondly, the 4th kind of cell blood sinus endotheliocyte (can be attached cell or suspension cell) placed growth in another petridish, make attached cell fully adherent (the growth characteristics difference of every kind of cell causes the adherent time that certain difference is arranged).
Once more; After treating that attached cell is adherent, remove unnecessary nutrient solution, 3 holes are cultivated support and growth altogether to be had the suspension type of 3 kinds of cells to cultivate cell to be assembled in growth and to have in the 4th kind of Tissue Culture Dish; Add fresh medium, highly make it can not have suspension type to cultivate the bottom of cell.Because suspension type is cultivated the aperture that different sizes are arranged at the bottom of cell, can allow liquid freely to pass through, and cell can not pass through, therefore, 4 kinds of cells can interconnect through nutrient solution, are in the same growing environment.Be defined as the time that 4 kinds of co-culture of cells begin this moment, according to different investigators' demand, in nutrient solution, adds exogenous fiddle factors.
Experiment according to investigator's requirement, can be collected the supernatant of co-culture system after finishing, and observes the variation of secreted protein in the co-culture system; Different suspension type petridish is taken out, collect 4 kinds of different cells respectively, be used for next step experiment.
Cultivating support altogether with 3 holes is example to the common cultivation of 4 kinds of cells:
Shown in Fig. 2 (a), Fig. 2 (b); One kind of multiple co-culture of cells supports; This support comprises stent cover 1 and the bracing frame 2 that is connected with said stent cover 1, offers fixed orifices 3 on the described stent cover 1, and described stent cover 1 can be located to be connected with described bracing frame 2 on the edge of.The edge of described fixed orifices 3 is provided with open slot 31.Described fixed orifices is 3.Described open slot 31 is 3.The height of described bracing frame 2 is for being 19mm.
As shown in Figure 3, one kind of multiple cell co-culture devices, this device comprise petridish 4 and are positioned over the common cultivation support in the petridish that this is cultivated support altogether and comprises stent cover and the bracing frame that is connected with said stent cover, offers fixed orifices on the described stent cover.The edge of described fixed orifices is provided with open slot.This device also includes the cultivation cell.Described cultivation cell is a capsule, and the edge of capsule is provided with the stationary installation of mating with said open slot.
Above-mentioned petridish can adopt 10 centimetres of U.S. corning company diameters, highly is the petridish of 2 centimetres or equal specification; Cultivating cell adopts millipore company suspension type to cultivate cell.Because the utility model is cultivated the height of the bracing frame of support and is designed to 19mm, therefore will be loaded with the cultivation support of cultivating cell and put into petridish, certain distance is left with the petridish bottom in this cultivation cell bottom, can directly not contact each other.Described petridish, cultivate support and suspension type altogether and cultivate cell and then become a co-culture system that can carry out the various kinds of cell co-cultivation, each suspension type is cultivated in cell can add different types of cell (can be attached cell or suspension cell) respectively.Below 4 kinds of cell co-cultivation are described.
At first; With 3 kinds of different types of cells (can be attached cell or suspension cell); Respectively put into the cultivation cell of common cultivation support like liver cell, scavenger cell and stellate cell; Again this support is put into petridish, place cell culture incubator to cultivate for some time (6 hours or incubated overnight) whole co-culture system, make attached cell fully adherent (the growth characteristics difference of every kind of cell causes the adherent time that certain difference is arranged).Cultivating the liquid that can add 2 to 3 milliliters in the cell, is 1 * 10 with density
7The cell of/milliliter is an example, can add 2 to 3 * 10
7Individual cell needing to be suitable for the experiment of a large amount of cells.Respectively cultivate between the cell cell this moment and independently grow, nutrient solution does not interconnect yet.
Secondly, the 4th kind of cell blood sinus endotheliocyte (can be attached cell or suspension cell) placed growth in another petridish, make attached cell fully adherent (the growth characteristics difference of every kind of cell causes the adherent time that certain difference is arranged).
Once more; After treating that attached cell is adherent, remove unnecessary nutrient solution, 3 holes are cultivated support and growth altogether to be had the suspension type of 3 kinds of cells to cultivate cell to be assembled in growth and to have in the 4th kind of Tissue Culture Dish; Add fresh medium, highly make it can not have suspension type to cultivate the bottom of cell.Because suspension type is cultivated the aperture that different sizes are arranged at the bottom of cell, can allow liquid freely to pass through, and cell can not pass through, therefore, 4 kinds of cells can interconnect through nutrient solution, are in the same growing environment.Be defined as the time that 4 kinds of co-culture of cells begin this moment, according to different investigators' demand, in nutrient solution, adds exogenous fiddle factors.
Experiment according to investigator's requirement, can be collected the supernatant of co-culture system after finishing, and observes the variation of secreted protein in the co-culture system; Different suspension type petridish is taken out, collect 4 kinds of different cells respectively, be used for next step experiment.
Cultivate support altogether with above-mentioned three holes and co-culture device is similar, the co-cultivation that support can be applicable to 4 kinds or 5 kinds cells is cultivated in four holes altogether, and the utility model cultivates support altogether and co-culture device can be expanded the co-cultivation that is applied to cell more than 5 kinds.
Obviously, the foregoing description of the utility model only be for explain clearly that the utility model does for example, and be not to be qualification to the embodiment of the utility model.For the those of ordinary skill in affiliated field, on the basis of above-mentioned explanation, can also make other multi-form variation or change.Here can't give exhaustive to all embodiments.Conspicuous variation that every technical scheme that belongs to the utility model is extended out or change still are in the row of the protection domain of the utility model.
Claims (10)
1. various kinds of cell is cultivated support altogether, it is characterized in that, this support comprises stent cover and the bracing frame that is connected with said stent cover, offers fixed orifices on the described stent cover.
2. various kinds of cell according to claim 1 is cultivated support altogether, it is characterized in that, described stent cover edge is connected with bracing frame.
3. various kinds of cell according to claim 1 and 2 is cultivated support altogether, it is characterized in that, the edge of described fixed orifices is provided with open slot.
4. various kinds of cell according to claim 1 is cultivated support altogether, it is characterized in that, described fixed orifices is at least 2.
5. various kinds of cell according to claim 3 is cultivated support altogether, it is characterized in that, described open slot is 3.
6. various kinds of cell according to claim 1 is cultivated support altogether, it is characterized in that, the height of described bracing frame is for being 17-20mm.
7. various kinds of cell co-culture device; It is characterized in that; This device comprises that petridish and the many cells that are positioned in the petridish cultivate support altogether, and these many cells are cultivated support altogether and comprised stent cover and the bracing frame that is connected with said stent cover, offer fixed orifices on the described stent cover.
8. various kinds of cell co-culture device according to claim 7 is characterized in that the edge of described fixed orifices is provided with open slot.
9. various kinds of cell co-culture device according to claim 8 is characterized in that this device also includes the cultivation cell.
10. various kinds of cell co-culture device according to claim 8 is characterized in that, described many cells are cultivated the height of the height of support greater than said cultivation cell altogether, and less than the height of described petridish.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102952751A (en) * | 2011-08-23 | 2013-03-06 | 首都医科大学附属北京友谊医院 | Co-culture support of multiple types of cells |
| CN103710263A (en) * | 2012-09-28 | 2014-04-09 | 江苏吉锐生物技术有限公司 | Cell culture apparatus |
| CN104379723A (en) * | 2012-10-19 | 2015-02-25 | 韩国生命工学研究院 | Cell culture container having dual structure, and circulation culture system using same |
| CN109642200A (en) * | 2016-08-18 | 2019-04-16 | 普兰德有限两合公司 | Ware and the device for cultivating cell are inserted into cell culture |
| CN110616140A (en) * | 2019-10-28 | 2019-12-27 | 上海国睿生命科技有限公司 | Cell co-culture dish |
| US11732232B2 (en) | 2020-08-14 | 2023-08-22 | Korea Research Institute Of Bioscience And Biotechnology | Biomimetic cell culture apparatus and cell culture system comprising the same |
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2011
- 2011-08-23 CN CN2011203078457U patent/CN202181306U/en not_active Expired - Lifetime
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102952751A (en) * | 2011-08-23 | 2013-03-06 | 首都医科大学附属北京友谊医院 | Co-culture support of multiple types of cells |
| CN103710263A (en) * | 2012-09-28 | 2014-04-09 | 江苏吉锐生物技术有限公司 | Cell culture apparatus |
| CN103710263B (en) * | 2012-09-28 | 2015-05-27 | 江苏吉锐生物技术有限公司 | Cell culture apparatus |
| CN104379723A (en) * | 2012-10-19 | 2015-02-25 | 韩国生命工学研究院 | Cell culture container having dual structure, and circulation culture system using same |
| CN109642200A (en) * | 2016-08-18 | 2019-04-16 | 普兰德有限两合公司 | Ware and the device for cultivating cell are inserted into cell culture |
| CN110616140A (en) * | 2019-10-28 | 2019-12-27 | 上海国睿生命科技有限公司 | Cell co-culture dish |
| US11732232B2 (en) | 2020-08-14 | 2023-08-22 | Korea Research Institute Of Bioscience And Biotechnology | Biomimetic cell culture apparatus and cell culture system comprising the same |
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