CN205011768U - Novel embryo culture ware - Google Patents
Novel embryo culture ware Download PDFInfo
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- CN205011768U CN205011768U CN201520643393.8U CN201520643393U CN205011768U CN 205011768 U CN205011768 U CN 205011768U CN 201520643393 U CN201520643393 U CN 201520643393U CN 205011768 U CN205011768 U CN 205011768U
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Abstract
The utility model relates to a novel embryo culture ware, place the pole including culture dish main part, culture dish lid and embryo, the embryo places the pole and includes two non -toxicity thin slices of nai low temperature elasticity that are provided with 1-3 recess going out to the lateral process on it, and the embryo places the pole and comprises two non -toxicity thin slices of nai low temperature elasticity of symmetry laminating, two relative recesses component confined embryo standing grooves on two non -toxicity thin slices of nai low temperature elasticity, and it is division sieve network structure who has the several aperture that the embryo places the cell wall, still being provided with in the culture dish main part and trading the cistern, the culture dish main part is used for placing the draw -in groove that the embryo placed the pole with trading to correspond to be provided with on the cistern, the embryo place the pole arrange in the culture dish main part with trade in the draw -in groove on the cistern and form in low outer high tilt state, embryo's standing groove that the embryo placed the pole is located and trades the cistern. Use the utility model discloses, can change the culture solution through trading the cistern at the embryo culture in -process, need not change together together with the culture dish, the in -process of use is more convenient.
Description
Technical field
The utility model relates to a kind of novel embryo culture ware.
Background technology
Vitro culture is a key link in IVF (IVFET) technology, and the external environment of embryo growth directly affects growing of body early embryo, and then has influence on the plantation success ratio of embryo.Vitro culture needs to use embryo culture ware, and the embryo culture ware used at present is the culture dish insufficiency of function of the simple structure be made up of culture dish main body and culture dish lid, this structure, needs to change together with culture dish when changing nutrient solution.This is because: polycyesis all can increase the hypertension of disease risks as pregnant woman to pregnant woman and fetus, fetal anomaly etc., present supplementary reproduction medical circle advocates single pregnancy, it mainly implements means is that single blastaea is transplanted, therefore after supplementary reproduction operation obtains the feritilization of ovum, embryo need be cultured to blastula stage in vitro, and then transplants.Material needed for the growth course of embryo is not identical in cleavage stage and blastula stage, it is main for mainly consuming pyruvic acid cleavage stage embryo, when being developed to blastula stage, metabolism changes glycolysis-into by tricarboxylic acid cycle, according to the feature of above fetal development, the nutrient solution composition of different times can change to some extent, and therefore cleavage stage is cultivated to convert to cultivate blastula stage needs to change nutrient solution often.But embryo culture ware structure is simple at present, cleavage stage nutrient solution is used to make the droplet of several 20 ~ 50uml and cover cultivation oil primarily of staff during cultivation, be placed in incubator and carry out gas, the balance of pH value, after having balanced, zygote is placed in droplet to cultivate, cultivates the change that oil mainly prevents Medium drop osmotic pressure.The usual way changing nutrient solution gets out to the day before yesterday need changing liquid the microtitre plate that blastula stage, nutrient solution made in fetal development, and be placed in incubator and carry out gas, the balance of pH value, after having balanced, proceeded to needing the embryo carrying out blastocyst culture in droplet by staff.This cultural method is also sequential culture, need reconfigure a culture dish when changing liquid, and needs the second people to check the information changing culture dish, and these intricate operation, easily make mistakes.
Summary of the invention
The technical problems to be solved in the utility model is: provide a kind of and use more convenient novel embryo culture ware, can change nutrient solution, not needing to change together with culture dish, solving above-mentioned prior art Problems existing in embryo culture process by changing liquid bath.
The technical scheme solved the problems of the technologies described above is: a kind of novel embryo culture ware, comprise culture dish main body and culture dish lid, this novel embryo culture ware also includes N root embryo and places bar, described embryo places bar and comprises the low temperature resistant elastic sheet of two panels nontoxicity, the low temperature resistant elastic sheet of every sheet nontoxicity is provided with 1 ~ 3 outstanding laterally groove, it is that the low temperature resistant elastic sheet of two panels nontoxicity of being fitted by symmetry is formed that embryo places bar, two grooves relative on the low temperature resistant elastic sheet of two panels nontoxicity form the embryo's standing groove closed, it is the mesh-like structure having several aperture that embryo places cell wall, also be provided with in described culture dish main body and change liquid bath, this changes liquid bath center and overlaps with culture dish main center, culture dish main body and to change on liquid bath and be correspondingly provided with the draw-in groove placing bar for placing embryo, change draw-in groove bottom face on liquid bath lower than the draw-in groove bottom face in culture dish main body, embryo places bar and to be placed in culture dish main body and to change on liquid bath in draw-in groove and to be in heeling condition, embryo's standing groove of embryo's placement bar is positioned at and changes liquid bath, and the value of N is 1 ~ 20.
This novel embryo culture ware also includes N root and places by removably and embryo the holding rod that bar is connected.
Further technical scheme of the present utility model is: every low temperature resistant elastic sheet of sheet nontoxicity is provided with 2 ~ 3 outstanding laterally grooves, and the distance L between adjacent two embryo's standing grooves is 2 ~ 3mm.
Owing to adopting said structure, the novel embryo culture ware of the utility model has following beneficial effect:
1, the utility model is more convenient in the process used.In embryo culture process, can changing culture dish, changing nutrient solution by changing liquid bath, embryo is placed in can be more convenient in the process of embryo transfer and frozen embryo on embryo's placement bar.Because culture dish is not changed, the information being marked on culture dish does not also change, and is therefore not easy to make mistakes.
2, when embryo cultivate in culture dish end need to carry out freezing time, embryo can be placed bar outer end and connect upper holding rod and can form embryo vitrifying freeze and carry bar (as shown in Figure 9), be directly used in embryo cryopreservation operation.Do not need again embryo to be transferred to other embryo vitrifying freeze from embryo's placement bar to carry bar, therefore use procedure is fast very convenient.
What 3, the utility model embryo placed pole pair embryo is fixedly adopt the low temperature resistant elastic sheet of the two panels nontoxicity with enlarged area to realize, without in external force situation, the low temperature resistant elastic sheet of two nontoxicitys is closure state, external force just can allow the low temperature resistant elastic sheet of two panels nontoxicity open (as shown in Figs. 7-8) more a little, and operation is very easy to place embryo.
Below, in conjunction with the accompanying drawings and embodiments the technical characteristic of the novel embryo culture ware of the utility model is further described.
Accompanying drawing explanation
Fig. 1: the novel embryo culture ware structural representation of the utility model.
Fig. 2: the culture dish main body of the utility model and change liquid trough structure schematic diagram.
Fig. 3: the embryo of the utility model places bar front view.
Fig. 4: the embryo of the utility model places bar vertical view.
Fig. 5: the embryo of the utility model places bar structural representation (being provided with 3 embryo's standing grooves).
Fig. 6: the mesh structure schematic diagram of embryo's standing groove of the utility model.
Fig. 7: embryo's standing groove structural representation (without the closed state of external force, amplifying) of the utility model.
Fig. 8: embryo's standing groove structural representation (have the open mode of external force, amplify) of the utility model.
Fig. 9: the embryo of the utility model places bar and holding rod connection diagram.
In figure: 1-culture dish main body, 2-embryo places bar, the low temperature resistant elastic sheet of 21-nontoxicity, 22-embryo standing groove, 23-aperture, and 3-changes liquid bath, 4-draw-in groove, 5-holding rod.
Embodiment
Embodiment 1: a kind of novel embryo culture ware, as Figure 1-Figure 2, comprise culture dish main body 1 and culture dish lid is (not shown in FIG., its structure is same as the prior art), this novel embryo culture ware also includes 8 embryos and places bar 2, described embryo places bar 2(as shown in figures 3 to 6) comprise the low temperature resistant elastic sheet 21 of two panels nontoxicity, the low temperature resistant elastic sheet of every sheet nontoxicity is provided with 1 ~ 3 outstanding laterally groove, it is that the low temperature resistant elastic sheet 21 of two panels nontoxicity of being fitted by symmetry is formed that embryo places bar 2, two grooves relative on the low temperature resistant elastic sheet of two panels nontoxicity form the embryo's standing groove 22 closed, it is the mesh-like structure having several aperture 23 that embryo places cell wall, nutrient solution and cryoprotectant can be worked to embryo by the mesh-like structure on embryo's standing groove, also be provided with in described culture dish main body 1 and change liquid bath 3, this changes liquid bath center and overlaps with culture dish main center, the culture dish principal space changing liquid bath and its outside is disconnected, culture dish main body and to change on liquid bath and be correspondingly provided with the draw-in groove 4 placing bar for placing embryo, change draw-in groove bottom face on liquid bath lower than the draw-in groove bottom face in culture dish main body, embryo places bar 2 and is placed in culture dish main body and changes low outer high heeling condition in the interior also formation of draw-in groove 4 on liquid bath, embryo's standing groove 22 of embryo's placement bar is positioned at and changes liquid bath 3, to ensure that embryo can obtain the immersion of nutrient solution.This novel embryo culture ware also includes 8 and places by removably and embryo the holding rod 5 that bar 2 is connected, when cultivating end and needing freezing, described holding rod 5 and embryo are placed bar 2 and are connected by removably, grafting, buckle etc. such as can be adopted to be convenient to connect again easy-to-dismount removably connect, so that refrigeration operation.
When the low temperature resistant elastic sheet of every sheet nontoxicity being provided with 2 ~ 3 outstanding laterally grooves, distance L between adjacent two embryo's standing grooves 22 is 2 ~ 3mm, as one conversion, also distance between adjacent two embryo's standing grooves 22 can be regulated according to practical situation.
As one conversion, also can not configure holding rod 5, adopt other year bar or other mode to carry out freezing.
As one conversion, the quantity that described embryo places bar 2 can be arranged according to practical situation, generally can be 1 ~ 20.
Use procedure: during embryo culture, first add nutrient solution novel changing in liquid bath of embryo culture ware, place embryo culture case inner equilibrium, the embryo after treating nutrient solution balance embryo being transferred to novel embryo culture ware places on bar and cultivates, when nutrient solution need be changed in midway, the nutrient solution balanced can be changed by changing liquid bath.The design of the novel embryo culture ware of the utility model is embryo can be fixed in culture dish, when changing liquid, direct suction pipe is by liquid waste sucking-off, put into the liquid that fresh equilibration completes, repeat for several times, because culture dish is not changed, the information being marked on culture dish does not also change, and is therefore not easy to make mistakes.
Claims (3)
1. a novel embryo culture ware, comprise culture dish main body (1) and culture dish lid, it is characterized in that: this novel embryo culture ware also includes N root embryo and places bar (2), described embryo places bar (2) and comprises the low temperature resistant elastic sheet of two panels nontoxicity (21), the low temperature resistant elastic sheet of every sheet nontoxicity is provided with 1 ~ 3 outstanding laterally groove, it is that the low temperature resistant elastic sheet of two panels nontoxicity (21) of being fitted by symmetry is formed that embryo places bar (2), two grooves relative on the low temperature resistant elastic sheet of two panels nontoxicity form the embryo's standing groove (22) closed, it is the mesh-like structure having several aperture (23) that embryo places cell wall, also be provided with in described culture dish main body (1) and change liquid bath (3), this changes liquid bath center and overlaps with culture dish main center, culture dish main body and to change on liquid bath and be correspondingly provided with the draw-in groove (4) placing bar for placing embryo, change draw-in groove bottom face on liquid bath lower than the draw-in groove bottom face in culture dish main body, embryo places bar (2) and to be placed in culture dish main body and to change on liquid bath in draw-in groove (4) and to be in heeling condition, embryo's standing groove (22) of embryo's placement bar is positioned at and changes liquid bath (3), and the value of N is 1 ~ 20.
2. novel embryo culture ware according to claim 1, is characterized in that: this novel embryo culture ware also includes N root and places by removably and embryo the holding rod (5) that bar (2) is connected.
3. novel embryo culture ware according to claim 1 and 2, is characterized in that: every low temperature resistant elastic sheet of sheet nontoxicity is provided with 2 ~ 3 outstanding laterally grooves, and the distance L between adjacent two embryo's standing grooves (22) is 2 ~ 3mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520643393.8U CN205011768U (en) | 2015-08-25 | 2015-08-25 | Novel embryo culture ware |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520643393.8U CN205011768U (en) | 2015-08-25 | 2015-08-25 | Novel embryo culture ware |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN205011768U true CN205011768U (en) | 2016-02-03 |
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ID=55210382
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201520643393.8U Expired - Fee Related CN205011768U (en) | 2015-08-25 | 2015-08-25 | Novel embryo culture ware |
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| Country | Link |
|---|---|
| CN (1) | CN205011768U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110387317A (en) * | 2019-08-29 | 2019-10-29 | 贵州大学 | A kind of pathogenic experiment tieback ware |
-
2015
- 2015-08-25 CN CN201520643393.8U patent/CN205011768U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110387317A (en) * | 2019-08-29 | 2019-10-29 | 贵州大学 | A kind of pathogenic experiment tieback ware |
| CN110387317B (en) * | 2019-08-29 | 2023-06-20 | 贵州大学 | Tieback ware for pathogenicity experiment |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160203 Termination date: 20170825 |