CN203923211U - A kind of co-culture of cells orifice plate - Google Patents
A kind of co-culture of cells orifice plate Download PDFInfo
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- CN203923211U CN203923211U CN201420342584.6U CN201420342584U CN203923211U CN 203923211 U CN203923211 U CN 203923211U CN 201420342584 U CN201420342584 U CN 201420342584U CN 203923211 U CN203923211 U CN 203923211U
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- culture
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- cells
- orifice plate
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Abstract
The utility model relates to a kind of co-culture of cells orifice plate, comprises several interconnective culture hole, between adjacent culture hole, is provided with through hole, and described through hole is by piston packing.Through hole is square opening.Under through hole along with the distance A=1.2～2mm of culture hole bottom.The utility model has the advantages that: through hole has reliable sealing, piston is detachable, having realized multiple attached parietal cell noncontact cultivates altogether, be conducive to realize various kinds of cell grows in same culture system, and carry out exchange of substance between cell and cell, cell and culture environment, influence each other, interact, thereby reach the effect that realizes simulated in vivo environment.
Description
Technical field
The utility model relates to a kind of co-culture of cells orifice plate, relates in particular to one and is applicable to different sorts cell noncontact cultured cells cultivation orifice plate altogether.
Background technology
External many cells cultivate be altogether by different sorts cell cultures in same environment, make iuntercellular information communicate with each other, connect each other, interact, thereby the cell micro-environment in analogue body as far as possible, be reached for fundamental research, drug researches etc. provide more effectively, reliably, experimental result accurately.
Co-culture of cells mainly contains two kinds of methods: Co-culture system and indirectly co-culture system.Co-culture system is that 2 kinds or cell of more than two kinds are inoculated in same inventionculture tools simultaneously, and between dissimilar cell, directly contact is cultivated.Indirectly co-culture system, is about to 2 kinds or cell of more than two kinds and is inoculated in respectively on different carriers, then these two kinds of carriers is placed among same culture environment, makes different types of cell share same culture systems and not directly contact.
(1) after the various kinds of cell cultivation due to Co-culture system, cannot separate and carry out follow-up study, so can not meet current experiment demand.
(2) the indirect co-culture system equipment of current cells involved is most widely used is cell culture insert.As the Transwell co-culture system of healthy and free from worry (Corning) company.Transwell co-culture system is the cup-formed device that has permeability with a kind of, a film with permeability is put in cup bottom, pore size is 0.1～12.0 μ m, Transwell cell is put into culture plate, A cell kind is upper indoor, lower floor plantation B cell, realizes the common cultivation of two kinds of cells, thereby can study effect and the impact on cell of material that emiocytosis or metabolism produce.The drawback of this method is: 1, can only carry out two kinds of co-culture of cells, cannot cultivate altogether by many cells.2, in two kinds of cells, there is one to be grown on film, carry out subsequent experimental inconvenience.3, carry out cell while changing liquid, operation inconvenience.4, expensive.
Summary of the invention
For overcoming the deficiencies in the prior art, the purpose of this utility model is to provide a kind of co-culture of cells orifice plate, realizes multiple attached parietal cell noncontact co-cultivation.
For achieving the above object, the utility model is achieved through the following technical solutions:
A kind of co-culture of cells orifice plate, comprises several interconnective culture hole, between adjacent culture hole, is provided with through hole,
Described through hole is by piston packing.
Described through hole is square opening.
Under described through hole along with the distance A=1.2～2mm of culture hole bottom.
Described piston is provided with handle.
Compared with prior art, the beneficial effects of the utility model are:
A, realize multiple attached parietal cell noncontact and cultivated altogether, be conducive to realize various kinds of cell and grow in same culture system, and carry out exchange of substance between cell and cell, cell and culture environment, influence each other, interact, thereby reach the effect that realizes simulated in vivo environment.
B, different types of cell grow in respectively in different culture hole, can not cause the pollution between different cells and mix, and are easy to harvested cell.
C, can extract RNA for the cell after common cultivation, protein etc. are for the related experiment such as gene and protein expression.
D, the variation that can study the morphological change of each co-culture of cells front and back and occur under different treatment factor (as disturbed, crossing expression etc.) effect.
E, for cell in vitro drug research.
F, raising conventional efficient, can regulate porous intercommunication according to demand, makes the cultivation orifice plate of different size, easy to operate.
G, through hole have reliable sealing, and piston is detachable.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Fig. 2 is the sectional view of Fig. 1 along A-A line.
Fig. 3 is piston structure schematic diagram.
In figure: 1-culture hole 2-through hole 3-piston 4-handle.
Embodiment
Below in conjunction with Figure of description, the utility model is described in detail, but should be noted that enforcement of the present utility model is not limited to following embodiment.
See Fig. 1-Fig. 3, a kind of co-culture of cells orifice plate, comprises several interconnective culture hole 1, between adjacent culture hole 1, is provided with through hole 2, and described through hole 2 is sealed by piston 3, the outline of piston 3 and through hole 2 wringing fits; Piston 3 materials are silica gel or identical with culture plate material.Through hole 2 is square opening.Distance A=1.2～the 2mm of 2 times edges of through hole and culture hole 1 bottom, facilitates cell cultures.Piston 3 is provided with handle 4, facilitates the plug of piston 3.
Embodiment:
Be total to culture plate as example taking 6 porocytes:
When making, get through adjacent two culture hole 1 of common flat 6 orifice plates, form square through hole 2, the long B=3-12mm in hole, the wide C=1.5-2.2mm in hole, the distance A=1.2～2mm of 2 times edges of through hole and culture hole 1 bottom.In through hole 2, add piston 3 and seal, make common cultivation 6 orifice plates.While needing between hole intercommunication, use aseptic nipper to pinch handle 4 and take out piston 3.
When use, taking 3 kinds of co-culture of cells as example:
First 3 kinds of cells are carried out to single culture: each cell is planted respectively at the special creep plate of 6 orifice plate cell (circular lid slide, TC processes, aseptic) on, need to determine growth population and the time of cell on slide according to experiment, cell takes out slide after growing, take off two pistons 3 of adjacent three culture hole 1 in co-culture of cells 6 orifice plates with aseptic nipper, realize three culture hole 1 intercommunications, there is the slide of 3 kinds of cells to put into respectively three culture hole 1 of intercommunication kind, add appropriate substratum to start to carry out co-culture of cells.(special cell climbing sheet can be selected different size as required)
Experiment finishes rear a, desirable culture system supernatant, for studying a series of researchs of culture system internal secretion albumen; B, can take out slide, cell extraction RNA, protein etc. on slide are carried out to a series of research; C, can carry out the experiments such as immunofluorescence.
Also can directly in co-culture of cells 6 orifice plates, plant desired number cell according to demand, after cell attachment, take out piston 3 with aseptic nipper, realize intercommunication many cells between hole and cultivate altogether afterwards.
Experiment finishes rear desirable supernatant etc. and carries out follow-up study.
After this equipment equal proportion scaling, can be made into as required the common culture plate in 12 holes or 24 holes and be total to culture plate etc.
The utility model has the advantages that:
A, realize multiple attached parietal cell noncontact and cultivated altogether, be conducive to realize various kinds of cell and grow in same culture system, and carry out exchange of substance between cell and cell, cell and culture environment, influence each other, interact, thereby reach the effect that realizes simulated in vivo environment.
B, different types of cell grow in respectively in different culture hole, can not cause the pollution between different cells and mix, and are easy to harvested cell.
C, can extract RNA for the cell after common cultivation, protein etc. are for the related experiment such as gene and protein expression.
D, the variation that can study the morphological change of each co-culture of cells front and back and occur under different treatment factor (as disturbed, crossing expression etc.) effect.
E, facilitate cell in vitro drug research.
F, raising conventional efficient, can regulate porous intercommunication according to demand, makes the cultivation orifice plate of different size, easy to operate.
G, through hole have reliable sealing, and piston is detachable.
H, employing and existing international culture plate profile specification, be convenient to produce and use.
Claims (4)
1. a co-culture of cells orifice plate, comprises several interconnective culture hole, it is characterized in that, between adjacent culture hole, be provided with through hole, described through hole is by piston packing.
2. a kind of co-culture of cells orifice plate according to claim 1, is characterized in that, described through hole is square opening.
3. a kind of co-culture of cells orifice plate according to claim 1, is characterized in that, under described through hole along with the distance A=1.2～2mm of culture hole bottom.
4. a kind of co-culture of cells orifice plate according to claim 1, is characterized in that, described piston is provided with handle, and piston material is silica gel or identical with culture plate material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420342584.6U CN203923211U (en) | 2014-06-24 | 2014-06-24 | A kind of co-culture of cells orifice plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420342584.6U CN203923211U (en) | 2014-06-24 | 2014-06-24 | A kind of co-culture of cells orifice plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203923211U true CN203923211U (en) | 2014-11-05 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420342584.6U Expired - Fee Related CN203923211U (en) | 2014-06-24 | 2014-06-24 | A kind of co-culture of cells orifice plate |
Country Status (1)
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| CN (1) | CN203923211U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774763A (en) * | 2015-04-24 | 2015-07-15 | 何向锋 | Microchip type single cell cloning separation culture plate and single cell cloning separation method |
| CN109097276A (en) * | 2018-08-17 | 2018-12-28 | 南通大学 | Non-contact cell co-culture device, manufacturing method and cell culture method |
| WO2021056921A1 (en) * | 2019-09-25 | 2021-04-01 | 西北农林科技大学 | Cell co-culture apparatus and co-culture method of bovine muscle cells and adipose cells |
-
2014
- 2014-06-24 CN CN201420342584.6U patent/CN203923211U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104774763A (en) * | 2015-04-24 | 2015-07-15 | 何向锋 | Microchip type single cell cloning separation culture plate and single cell cloning separation method |
| CN109097276A (en) * | 2018-08-17 | 2018-12-28 | 南通大学 | Non-contact cell co-culture device, manufacturing method and cell culture method |
| CN109097276B (en) * | 2018-08-17 | 2022-01-07 | 南通大学 | Non-contact cell co-culture device, manufacturing method and cell culture method |
| WO2021056921A1 (en) * | 2019-09-25 | 2021-04-01 | 西北农林科技大学 | Cell co-culture apparatus and co-culture method of bovine muscle cells and adipose cells |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141105 Termination date: 20190624 |
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| CF01 | Termination of patent right due to non-payment of annual fee |