CN204237802U - Contactless cell co-culture device - Google Patents
Contactless cell co-culture device Download PDFInfo
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- CN204237802U CN204237802U CN201420671064.XU CN201420671064U CN204237802U CN 204237802 U CN204237802 U CN 204237802U CN 201420671064 U CN201420671064 U CN 201420671064U CN 204237802 U CN204237802 U CN 204237802U
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- cell
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- engineering
- filtering membrane
- cavity volume
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- 238000003501 co-culture Methods 0.000 title claims abstract description 13
- 238000004113 cell culture Methods 0.000 claims abstract description 59
- 238000004519 manufacturing process Methods 0.000 claims abstract description 39
- 239000012528 membrane Substances 0.000 claims abstract description 29
- 238000001914 filtration Methods 0.000 claims abstract description 28
- 238000011081 inoculation Methods 0.000 claims abstract description 17
- 239000012531 culture fluid Substances 0.000 claims abstract description 4
- 239000003365 glass fiber Substances 0.000 claims description 8
- 229920001577 copolymer Polymers 0.000 claims description 5
- 230000004936 stimulating Effects 0.000 abstract description 3
- 230000001464 adherent Effects 0.000 abstract description 2
- 238000010276 construction Methods 0.000 abstract description 2
- 239000000725 suspension Substances 0.000 abstract description 2
- 210000004027 cells Anatomy 0.000 description 31
- 238000000034 method Methods 0.000 description 7
- 239000006285 cell suspension Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 235000015097 nutrients Nutrition 0.000 description 3
- 238000011031 large scale production Methods 0.000 description 2
- 102000004127 Cytokines Human genes 0.000 description 1
- 108090000695 Cytokines Proteins 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001681 protective Effects 0.000 description 1
- 230000003248 secreting Effects 0.000 description 1
- 238000003153 stable transfection Methods 0.000 description 1
- 210000000130 stem cell Anatomy 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 238000009736 wetting Methods 0.000 description 1
Abstract
The utility model discloses a kind of contactless cell co-culture device, comprise airtight housing, the cavity volume holding cell culture fluid is provided with in described housing, be provided with along short transverse the biological filtering membrane be convenient to cytokine and passed through in described cavity volume, described cavity volume is separated into the engineering cell cultivation region and production cell cultures district that are interconnected by described biological filtering membrane, and the volume in described production cell cultures district is greater than the volume of described engineering cell cultivation region; The top that described housing is positioned at described engineering cell cultivation region and described production cell cultures district is respectively equipped with engineering cell cultivation inoculation mouth and production cell cultures inoculation mouth, and described engineering cell cultivation inoculation mouth and production cell cultures are inoculated mouth and be respectively equipped with the lid be sealed and matched.The utility model overall construction design is reasonable, high-efficient culture can produce cell, cell rapid amplifying of stimulating production, and can realize engineering cell and be effectively separated with production cell, is applicable to the cell cultures of adherent growth or suspension growth.
Description
Technical field
The utility model relates to technical field of cell culture, is specifically related to a kind of contactless cell co-culture device.
Background technology
Cell cultures is link very important in biotechnology research and large-scale production process.For the scale operation of some stem cell or immunocyte, often need to add one or more cytokines, to reach the effect of rapid stimulation cell proliferation.But the cytokine cost purchasing these restructuring is in enormous quantities very high, is unfavorable for long-term large-scale production.Therefore, by utilizing slow virus stable transfection in engineering cell the expression plasmid of the above-mentioned factor, being synthesized by engineering cell and secreting these factors.But, if engineering cell with producing cell co-cultivation, then effectively cannot be separated in the later stage, therefore the cell product of purifying cannot be provided.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of reasonable in design, easy for operation, high-efficient culture can produce cell, cell rapid amplifying of stimulating production, and engineering cell can be realized and produce the contactless cell co-culture device that is effectively separated of cell.
For solving the problems of the technologies described above, the technical solution of the utility model is: contactless cell co-culture device, comprise airtight housing, the cavity volume holding cell culture fluid is provided with in described housing, be provided with along short transverse the biological filtering membrane be convenient to cytokine and passed through in described cavity volume, described cavity volume is separated into the engineering cell cultivation region and production cell cultures district that are interconnected by described biological filtering membrane, and the volume in described production cell cultures district is greater than the volume of described engineering cell cultivation region; The top that described housing is positioned at described engineering cell cultivation region and described production cell cultures district is respectively equipped with engineering cell cultivation inoculation mouth and production cell cultures inoculation mouth, and described engineering cell cultivation inoculation mouth and production cell cultures are inoculated mouth and be respectively equipped with the lid be sealed and matched.
As the improvement to technique scheme, described biological filtering membrane comprises glass fibre rete, and the both sides of described glass fibre rete cover respectively and are provided with nonwoven layer, and the outside of nonwoven layer described in two is coated with respectively and is covered with acrylic acid co-polymer layer.
As the improvement to technique scheme, described biological filtering membrane can regulate along the height of described cavity volume short transverse.
As the further improvement to technique scheme, described biological filtering membrane be arranged in parallel along described cavity volume short transverse.
As the further improvement to technique scheme, described biological filtering membrane is obliquely installed along described cavity volume short transverse.
As the further improvement to technique scheme, the cross-sectional shape of described housing is rectangle, square, circle, ellipse, rhombus or other are irregularly shaped.
The utility model, by adopting technique scheme, has following beneficial effect:
(1) by described biological filtering membrane described cavity volume is separated into the engineering cell cultivation region and production cell cultures district that are interconnected, engineering born of the same parents and production cell are placed in different compartments respectively, utilize biological filtering membrane by both nutrient solution UNICOMs, can ensure that the cytokine that engineering cell is secreted is passed through smoothly, the effect of cell rapid amplifying thus realization is stimulated production;
(2) by described biological filtering membrane by engineering born of the same parents with produce cell and be separated by and leave, mutually not by, be convenient to effectively be separated in the later stage, thus the cell product of purifying be provided;
(3) be greater than the volume of described engineering cell cultivation region by the volume in described production cell cultures district, can realize more efficiently cultivating production cell, improve culture effect and efficiency.
The utility model overall construction design is reasonable, easy for operation, high-efficient culture can produce cell, and the cell rapid amplifying and engineering cell being effectively separated with production cell of stimulating production, is applicable to the cell cultures of adherent growth or suspension growth.
Accompanying drawing explanation
The following drawings is only intended to schematically illustrate the utility model and explain, does not limit scope of the present utility model.Wherein:
Fig. 1 is the structural representation of the utility model embodiment one;
Fig. 2 is the schematic top plan view of the utility model embodiment one;
Fig. 3 is the local structure schematic diagram of biological filtering membrane;
Fig. 4 is the structural representation of the utility model embodiment two.
In figure: 1-housing; 2-cavity volume; 21-engineering cell cultivation region; 22-production cell cultures district; 3-biological filtering membrane; 31-glass fibre rete; 32-nonwoven layer; 33-acrylic acid co-polymer layer; 4-engineering cell cultivates inoculation mouth; 5-production cell cultures inoculation mouth; 6-lid.
Embodiment
Below in conjunction with drawings and Examples, set forth the utility model further.In the following detailed description, the mode only by illustrating describes some one exemplary embodiment of the present utility model.Undoubtedly, those of ordinary skill in the art can recognize, when not departing from spirit and scope of the present utility model, can revise by various different mode to described embodiment.Therefore, accompanying drawing is illustrative with being described in essence, instead of for limiting the protection domain of claim.
Embodiment one:
As shown in Figure 1 to Figure 3, contactless cell co-culture device, comprise airtight housing 1, the cross-sectional shape of housing 1 is rectangle, the cavity volume 2 holding cell culture fluid is provided with in described housing 1, be provided with along short transverse the biological filtering membrane 3 be convenient to cytokine and passed through in described cavity volume 2, but biological filtering membrane 3 can prevent cell from passing through, described cavity volume 2 is separated into the engineering cell cultivation region 21 and production cell cultures district 22 that are interconnected by described biological filtering membrane 3, the top that described housing 1 is positioned at described engineering cell cultivation region 21 and described production cell cultures district 22 is respectively equipped with engineering cell cultivation inoculation mouth 4 and production cell cultures inoculation mouth 5, described engineering cell cultivates inoculation mouth 4 and production cell cultures inoculation mouth 5 is respectively equipped with the lid 6 be sealed and matched.
Wherein, the volume in described production cell cultures district 22 is greater than the volume 21 of described engineering cell cultivation region, this structure design, can be beneficial to more efficiently cultivate production cell.
In the present embodiment; described biological filtering membrane 3 adopts following membrane structure: comprise glass fibre rete 31; the both sides of described glass fibre rete 31 cover respectively and are provided with nonwoven layer 32; the outside of nonwoven layer 32 described in two is coated with respectively and is covered with acrylic acid co-polymer layer 33; nonwoven layer 32 plays protective glass fiber rete 31 and acts on; acrylic acid co-polymer layer 33 can improve the wetting ability of filtering membrane, is convenient to infiltrate immediately, facilitates nutrient solution UNICOM to pass through.
In the present embodiment, described biological filtering membrane 3 can regulate along the height of described cavity volume 2 short transverse, and to facilitate the UNICOM's contact area between adjustment nutrient solution, and described biological filtering membrane 3 be arranged in parallel along described cavity volume 2 short transverse.
Using method is as follows: preparation engineering cell suspension and production cell suspension respectively.Engineering cell suspension is cultivated inoculation mouth 4 by engineering cell and is seeded to engineering cell cultivation region 21, production cell suspension is seeded to production cell cultures district 22 by production cell cultures inoculation mouth 5, engineering cell suspension and produce cell suspension by biological filtering membrane 3 communicating together, but cell not by.This device is placed in the CO of 37 DEG C
2cultivate in incubator.
Embodiment two:
As shown in Figure 4, unique difference of the present embodiment and embodiment one is: described biological filtering membrane 3 is obliquely installed along described cavity volume 2 short transverse, can when not increasing housing 1 volume, UNICOM's contact area in effective raising engineering cell cultivation region 21 and production cell cultures district 22, improve cytokine and pass through efficiency, to stimulate production cell rapid amplifying, improve culture effect.
Scope of the present utility model is not by the restriction of described specific embodiments, and described embodiment only for as the single example of illustrating the utility model all respects, also comprises method and the component of functional equivalent within the scope of the utility model.In fact, except content described herein, those skilled in the art can easily grasp multiple improvement of the present utility model with reference to description above and accompanying drawing.Described improvement also falls in the scope of appended claims.
Claims (6)
1. contactless cell co-culture device, comprise airtight housing, it is characterized in that: in described housing, be provided with the cavity volume holding cell culture fluid, be provided with along short transverse the biological filtering membrane be convenient to cytokine and passed through in described cavity volume, described cavity volume is separated into the engineering cell cultivation region and production cell cultures district that are interconnected by described biological filtering membrane, and the volume in described production cell cultures district is greater than the volume of described engineering cell cultivation region; The top that described housing is positioned at described engineering cell cultivation region and described production cell cultures district is respectively equipped with engineering cell cultivation inoculation mouth and production cell cultures inoculation mouth, and described engineering cell cultivation inoculation mouth and production cell cultures are inoculated mouth and be respectively equipped with the lid be sealed and matched.
2. contactless cell co-culture device as claimed in claim 1, it is characterized in that: described biological filtering membrane comprises glass fibre rete, the both sides of described glass fibre rete cover respectively and are provided with nonwoven layer, and the outside of nonwoven layer described in two is coated with respectively and is covered with acrylic acid co-polymer layer.
3. contactless cell co-culture device as claimed in claim 1, is characterized in that: described biological filtering membrane can regulate along the height of described cavity volume short transverse.
4. contactless cell co-culture device as claimed in claim 1, is characterized in that: described biological filtering membrane be arranged in parallel along described cavity volume short transverse.
5. contactless cell co-culture device as claimed in claim 1, is characterized in that: described biological filtering membrane is obliquely installed along described cavity volume short transverse.
6. the contactless cell co-culture device as described in any one of claim 1 to 5 claim, is characterized in that: the cross-sectional shape of described housing is rectangle, square, circle, ellipse, rhombus or other are irregularly shaped.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420671064.XU CN204237802U (en) | 2014-11-11 | 2014-11-11 | Contactless cell co-culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420671064.XU CN204237802U (en) | 2014-11-11 | 2014-11-11 | Contactless cell co-culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204237802U true CN204237802U (en) | 2015-04-01 |
Family
ID=52767319
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420671064.XU Active CN204237802U (en) | 2014-11-11 | 2014-11-11 | Contactless cell co-culture device |
Country Status (1)
| Country | Link |
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| CN (1) | CN204237802U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104877905A (en) * | 2015-05-04 | 2015-09-02 | 清华大学深圳研究生院 | Cell in-vitro co-culture microfluidic chip, system and method |
| CN105969652A (en) * | 2016-05-10 | 2016-09-28 | 程玉鹏 | Coculture apparatus for simulation of metabolic exchanges between plant suspension cells and endophytic fungi therein |
-
2014
- 2014-11-11 CN CN201420671064.XU patent/CN204237802U/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104877905A (en) * | 2015-05-04 | 2015-09-02 | 清华大学深圳研究生院 | Cell in-vitro co-culture microfluidic chip, system and method |
| CN105969652A (en) * | 2016-05-10 | 2016-09-28 | 程玉鹏 | Coculture apparatus for simulation of metabolic exchanges between plant suspension cells and endophytic fungi therein |
| CN105969652B (en) * | 2016-05-10 | 2019-11-19 | 程玉鹏 | Simulating plant suspension cell is metabolized the co-culture device exchanged with its endogenetic fungus |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |