CN203429183U - Three-dimensional cell culture plate - Google Patents
Three-dimensional cell culture plate Download PDFInfo
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- CN203429183U CN203429183U CN201320517079.6U CN201320517079U CN203429183U CN 203429183 U CN203429183 U CN 203429183U CN 201320517079 U CN201320517079 U CN 201320517079U CN 203429183 U CN203429183 U CN 203429183U
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- plate
- growth
- liquid storage
- hole
- cell culture
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Abstract
The utility model discloses a three-dimensional cell culture plate. The three-dimensional cell culture plate comprises a cell growth plate and a liquid storage plate, wherein the cell growth plate is installed on the upper part of the liquid storage plate; the lower surface of the cell growth plate is provided with a growth hole, the upper surface of the liquid storage plate is provided with a liquid storage hole, and the growth hole is matched with the liquid storage hole; the growth hole is provided with a sample inlet hole and an annular separation groove, the sample inlet hole is arranged in the center of the growth hole and penetrates through the growth hole, and the annular separation groove is arranged on the outer circumference of the sample inlet hole. The three-dimensional cell culture plate has the advantages of low cost, simplicity in operation and the like and can be used for the three-dimensional cell culture in an inverted culture method.
Description
Technical field
The utility model relates to a kind of Tissue Culture Plate, particularly relates to a kind of three-dimensional cell culture plate.
Background technology
Inversion culture method in three-dimensional cell cultural method, is cell suspending liquid to be suspended on to the lid inner side of culture dish, utilizes action of gravity, makes cell be positioned at drop downside liquid gas interface place, and cell is attached nowhere, interosculates, and forms thus three-dimensional micro-tissue.Be inverted culture method have simple and convenient, the size of micro-tissue can be by controlling the size of drop the advantages such as regulation and control.Yet, adopt existing culture dish to implement to be inverted culture technique, have some limitations: first, be inverted culture method and need skilled operative technique, drop is added drop-wise to the upper surface of culture dish, then when maintaining drop and not rolling, by the process of 180 ° of culture dish upsets, testing crew need to just can obtain the result of high multiplicity through long training; Secondly, limited in one's ability due to solid surface adsorptive liquid, is restricted the size of drop, and the nutriment that can provide is also limited, so is difficult to cultivate for a long time; Moreover, in culturing process, cannot change nutrient solution, cannot add medicine or other chemical reagent; In addition, after forming micro-tissue, also need to collect micro-tissue from the lid of culture dish, complex operation, can not be applicable to high-throughput drug examination link.
Utility model content
Based on this, be necessary that for existing culture dish, not being suitable for cell is inverted the defect of cultivating, provide a kind of cell that is applicable to be inverted the three-dimensional cell culture plate of cultivating.
A culture plate, comprises Growth of Cells plate and liquid storage plate, and described Growth of Cells plate is installed on the top of described liquid storage plate; The lower surface of described Growth of Cells plate is provided with growth hole, and the upper surface of described liquid storage plate is provided with liquid storage hole, and described growth hole and described liquid storage hole cooperatively interact; In described growth hole, be provided with sample holes and ring-type isolation channel, described sample holes is positioned at described growth Kong center and runs through wherein, and described ring-type isolation channel is positioned at the periphery of described sample holes.
In an embodiment, the aperture of the sample holes of described Growth of Cells plate is 0.1mm～2mm therein.
In an embodiment, the interior ring diameter of the ring-type isolation channel of described Growth of Cells plate is 3mm～10mm, more preferably 3mm～6mm therein.
Therein in an embodiment, the edge in the growth hole of described Growth of Cells plate is provided with ring-type draw-in groove, the edge of the liquid storage hole of described liquid storage plate is provided with collar flange, the ring-type draw-in groove in described growth hole and the collar flange of described liquid storage hole cooperatively interact, and make described growth hole correspondence be installed on the top of described liquid storage hole.
In an embodiment, the lower surface of described Growth of Cells plate is provided with a plurality of growths hole therein, and the upper surface of described liquid storage plate is provided with a plurality of liquid storage holes, and described growth hole and described liquid storage hole cooperatively interact.
In an embodiment, the growth hole of described Growth of Cells plate is identical with the liquid storage hole quantity of described liquid storage plate therein, and aperture is identical, and position is relative.
In an embodiment, described three-dimensional cell culture plate also comprises cover plate therein, and described cover plate is installed on the top of described Growth of Cells plate.
Three-dimensional cell culture plate described in the utility model, has with low cost, simple operation and other advantages, can be conveniently used in being inverted in culture method, carrying out three-dimensional cell cultivation.In three-dimensional cell culture plate described in the utility model, sample holes on Growth of Cells plate is used for injecting cell suspending liquid, and can be used for adding, change substratum and medicine, the aperture of sample holes is controlled in the scope of 0.1mm～2mm, guarantee that drop is under capillary effect on the one hand, can diffuse to the lower surface of Growth of Cells plate, can prevent that on the other hand drop from directly dropping in the liquid storage hole of below; Inject after cell suspending liquid, under the effect of Surface Tension of Liquid Drops, cell suspending liquid is suspended on the lower surface of Growth of Cells plate, and under action of gravity, cell is in the surface growth of liquid-gas and form three-dimensional cell tissue; Ring-type isolation channel on Growth of Cells plate, for controlling the contact area of cell suspending liquid and Growth of Cells plate, by regulating the interior ring diameter of ring-type isolation channel, the contact area of adjustable drop and Growth of Cells plate, thereby shape and the size of regulation and control drop, the drop of large volume can provide sufficient nutrient under the condition of not supplementing nutrient solution, by the shape of regulation and control drop, can control the forming process of three-dimensional cell tissue and form efficiency, the interior ring diameter of ring-type isolation channel is controlled to 3～10mm, can guarantees comparatively ideal droplet size and shape; Ring-type draw-in groove is set on Growth of Cells plate, on liquid storage plate, collar flange is set, ring-type draw-in groove and collar flange cooperatively interact, make the growth hole of Growth of Cells plate and the liquid storage hole of liquid storage plate mutually chimeric, the liquid in liquid storage hole can maintain ambient moisture, reduces the volatilization of droplet size, after treating that cell tissue has been cultivated, by simple vibrations, operate, can make the cell tissue of growing in hole fall into rapidly corresponding liquid storage hole, then directly carry out the experiments such as high throughput testing; In addition, cover plate is set on the top of Growth of Cells plate and forms enclosed space, can prevent from being polluted in cell cultivation process, and further stop and evaporate.
Accompanying drawing explanation
Fig. 1 is the structural representation of Growth of Cells plate described in the utility model;
Fig. 2 is the structural representation of liquid storage plate described in the utility model;
Fig. 3 is the cross-sectional view in growth described in the utility model hole;
Fig. 4 is the fundamental diagram of three-dimensional cell culture plate described in the utility model.
Embodiment
Below in conjunction with drawings and Examples, the utility model is described in further detail.
As shown in Figure 1 to Figure 3, three-dimensional cell culture plate described in the utility model comprises Growth of Cells plate 1, liquid storage plate 2 and cover plate (not shown), and Growth of Cells plate 1 is arranged on the top of liquid storage plate 2, and cover plate is arranged on the top of Growth of Cells plate 1.
The lower surface of Growth of Cells plate 1 is provided with a plurality of growths hole 11(for example 24 holes, 48 holes, 96 holes), growth Kong11 center is provided with the sample holes 12 through Growth of Cells plate 1, and the aperture of sample holes 12 is 0.1mm～2mm.The periphery of sample holes 12 is provided with ring-type isolation channel 13, and ring-type isolation channel 13 is opened on the lower surface of Growth of Cells plate 1, and the interior ring diameter of ring-type isolation channel 13 is 3mm～10mm, more preferably 3mm～6mm.The edge in growth hole 11 is also provided with ring-type draw-in groove 14, and ring-type draw-in groove 14 is positioned at the periphery of ring-type isolation channel 13, and ring-type draw-in groove 14 is opened on the lower surface of Growth of Cells plate 1.
The upper surface of liquid storage plate 2 is provided with a plurality of liquid storage hole 21(for example 24 holes, 48 holes, 96 holes), liquid storage hole 21 cooperatively interacts with growth hole 11, and both quantity is identical, aperture is identical, position is relative.The edge of liquid storage hole 21 is provided with collar flange 22, and collar flange 22 is positioned at the upper surface of liquid storage plate 2.The collar flange 22 of liquid storage hole 21 cooperatively interacts with the ring-type draw-in groove 14 in growth hole 11, thereby hole 11 correspondences that make to grow are installed on the top of liquid storage hole 21.
The principle of work of three-dimensional cell culture plate described in the utility model is as follows:
As shown in Figure 4, growth hole 11 is embedded in the top of liquid storage hole 21, the inwall injection cell suspending liquid 4 with pipettor along sample holes 12, and cell suspending liquid 4 is under capillary effect, attaching is suspended on the lower surface in growth hole 11, and is limited in the formed region of ring-type isolation channel 13; Under action of gravity, the cell aggregation in cell suspending liquid 4 is in the bottom of drop, thus promotion aggregation, and then form three-dimensional cell tissue; In liquid storage hole 21, add enough cell culture mediums 5, to keep there is sufficient moisture in the air of environment in culture plate, reduce the moisture evaporation of cell suspending liquid 4, guarantee that the osmotic pressure of cell suspending liquid 4 is constant, maintain the normal growth metabolism of cell.
Adopt three-dimensional cell culture plate described in the utility model to carry out cell and be inverted cultivation, without the culture dish that overturns by staff, form suspension drop, can reduce the impact that staff misoperation is cultivated three-dimensional cell; And in culturing process, can add easily, change nutrient solution and medicine, can improve the culture effect of being inverted culture method; After cultivation completes, vibrations culture plate, can obtain in the suspension of cell tissue, can directly carry out the subsequent experimental such as high throughput testing.
The above embodiment has only expressed several embodiment of the present utility model, and it describes comparatively concrete and detailed, but can not therefore be interpreted as the restriction to the utility model the scope of the claims.It should be pointed out that for the person of ordinary skill of the art, without departing from the concept of the premise utility, can also make some distortion and improvement, these all belong to protection domain of the present utility model.Therefore, the protection domain of the utility model patent should be as the criterion with claims.
Claims (7)
1. a three-dimensional cell culture plate, is characterized in that: comprise Growth of Cells plate and liquid storage plate, described Growth of Cells plate is installed on the top of described liquid storage plate; The lower surface of described Growth of Cells plate is provided with growth hole, and the upper surface of described liquid storage plate is provided with liquid storage hole, and described growth hole and described liquid storage hole cooperatively interact; In described growth hole, be provided with sample holes and ring-type isolation channel, described sample holes is positioned at described growth Kong center and runs through wherein, and described ring-type isolation channel is positioned at the periphery of described sample holes.
2. three-dimensional cell culture plate according to claim 1, is characterized in that: the aperture of the sample holes of described Growth of Cells plate is 0.1mm～2mm.
3. three-dimensional cell culture plate according to claim 2, is characterized in that: the interior ring diameter of the ring-type isolation channel of described Growth of Cells plate is 3mm～10mm.
4. three-dimensional cell culture plate according to claim 1, it is characterized in that: the edge in the growth hole of described Growth of Cells plate is provided with ring-type draw-in groove, the edge of the liquid storage hole of described liquid storage plate is provided with collar flange, the ring-type draw-in groove in described growth hole and the collar flange of described liquid storage hole cooperatively interact, and make described growth hole correspondence be installed on the top of described liquid storage hole.
5. the three-dimensional cell culture plate described in one of them according to claim 1 to 4, it is characterized in that: the lower surface of described Growth of Cells plate is provided with a plurality of growths hole, the upper surface of described liquid storage plate is provided with a plurality of liquid storage holes, and described growth hole and described liquid storage hole cooperatively interact.
6. three-dimensional cell culture plate according to claim 5, is characterized in that: the growth hole of described Growth of Cells plate is identical with the liquid storage hole quantity of described liquid storage plate, and aperture is identical, and position is relative.
7. the three-dimensional cell culture plate described in one of them according to claim 1 to 4, is characterized in that: described three-dimensional cell culture plate also comprises cover plate, and described cover plate is installed on the top of described Growth of Cells plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320517079.6U CN203429183U (en) | 2013-08-22 | 2013-08-22 | Three-dimensional cell culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320517079.6U CN203429183U (en) | 2013-08-22 | 2013-08-22 | Three-dimensional cell culture plate |
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| Publication Number | Publication Date |
|---|---|
| CN203429183U true CN203429183U (en) | 2014-02-12 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201320517079.6U Expired - Fee Related CN203429183U (en) | 2013-08-22 | 2013-08-22 | Three-dimensional cell culture plate |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106554937A (en) * | 2016-10-26 | 2017-04-05 | 南方医科大学珠江医院 | A kind of hepatocellular external three-dimensional culture method |
| CN116690866A (en) * | 2023-04-28 | 2023-09-05 | 南方科技大学 | Culture plate for 3D cell sphere culture, preparation method and culture method |
-
2013
- 2013-08-22 CN CN201320517079.6U patent/CN203429183U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106554937A (en) * | 2016-10-26 | 2017-04-05 | 南方医科大学珠江医院 | A kind of hepatocellular external three-dimensional culture method |
| CN106554937B (en) * | 2016-10-26 | 2019-08-27 | 南方医科大学珠江医院 | A kind of external three-dimensional culture method of liver cell |
| CN116690866A (en) * | 2023-04-28 | 2023-09-05 | 南方科技大学 | Culture plate for 3D cell sphere culture, preparation method and culture method |
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| Date | Code | Title | Description |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140212 |