CN203625382U - Oscillating large-scale suspension cell culture device - Google Patents
Oscillating large-scale suspension cell culture device Download PDFInfo
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- CN203625382U CN203625382U CN201320659669.2U CN201320659669U CN203625382U CN 203625382 U CN203625382 U CN 203625382U CN 201320659669 U CN201320659669 U CN 201320659669U CN 203625382 U CN203625382 U CN 203625382U
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- cell culture
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- suspension cell
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Abstract
The utility model relates to an oscillating large-scale suspension cell culture device comprising a rack and a bassinet arranged on the rack, wherein the rack is provided with an oscillating motor for driving the bassinet to oscillate; a culture flask is loaded in the bassinet and is provided with a culture solution inlet and a waste solution outlet; the culture solution inlet and the waste solution outlet are respectively provided with a semi-permeable membrane. The bassinet oscillates back and forth to enable a culture solution in the culture flask to form a wave motion which takes favorable oxygen supplying and mixing effects for cells and the culture solution, a shear force generated by the motion way is far smaller than that of a stirring type mixing system, and the cell injury is greatly reduced; meanwhile, the culture flask is provided with the culture solution inlet and the waste solution outlet, and a fresh culture solution can be used for continuously compensating the discharged waste solution containing metabolic products, so that enough nutrients are provided for the growth of the cells, and the culture efficiency is increased. The oscillating large-scale suspension cell culture device can be applied to the field of cell culture.
Description
Technical field
The utility model relates to field of cell culture, particularly relates to the extensive suspension cell culture device of a kind of swing type.
Background technology
Animal cell culture is widely used in biotechnology and biological medicine research.Conventionally, the production that animal cell culture is virus vaccines provides culture medium, is also that multiple biologics is produced indispensable instrument simultaneously, comprises monoclonal antibody and gene therapy product etc.In order to study in bio-chemical pathway, viral product, pathology and the cell in body or iuntercellular reaction etc., animal cell culture has also obtained application in a large amount of biological detection systems in addition.
Zooblast is different from vegetable cell and microorganism cells, and zooblast does not have cell walls, therefore, very sensitive to shearing force in the time cultivating, and requires also more strict.Traditional zooblast adopts square vase and rolling bottle to cultivate, and the technology of this respect is ripe, and still, the method exists the shortcomings such as cell density is low, virus yield is low, production cost is high, labour intensity is large, can not meet the requirement of modern biological seedling.The suspension culture technology of being born in the sixties in 20th century can be carried out mass cell cultivation, can obtain a large amount of viral product and high-quality vaccine product, generally application in production of vaccine abroad.
The mechanical agitation type bio-reaction system generally using in suspension culture technology have structure and simple to operate, be easy to amplify, cell harvesting is convenient, cell culture environment is easy to detect and controls and the feature such as optimization, but animal cell culture chamber is to shearing force sensitivity, therefore use mechanical stirring can cause damage to cell undoubtedly, the effect that impact is cultivated.
Utility model content
In order to overcome above-mentioned technical problem, the purpose of this utility model be to provide a kind of culture efficiency high, culturing cell is damaged to the extensive suspension cell culture device of little swing type.
The technical scheme that the utility model adopts is:
The extensive suspension cell culture device of swing type, comprise frame and rack-mounted cradle, in described frame, be provided with the oscillating motor for driving cradle to swing, in described cradle, be mounted with culturing bottle, described culturing bottle is provided with nutrient solution entrance and waste liquid outlet, and described nutrient solution entrance and waste liquid outlet place are provided with semi-permeable membranes.
As the further improvement of technique scheme, in described oscillating motor or frame, be provided with the controller of adjusting oscillating motor rotating speed.
As the further improvement of technique scheme, described nutrient solution entrance is connected to liquid-inlet pipe, and described waste liquid outlet is connected to liquid discharge pipe, and shut off valve is all installed on described liquid-inlet pipe and liquid discharge pipe, and described each shut off valve is electrically connected with controller.
As the further improvement of technique scheme, under meter is all installed on described liquid-inlet pipe and liquid discharge pipe, described each under meter is electrically connected with controller.
As the further improvement of technique scheme, on described liquid-inlet pipe, be connected to air intake branch, in described air intake branch, be provided with waterproof ventilated membrane.
As the further improvement of technique scheme, described cradle two ends are respectively equipped with the through hole that supplies liquid-inlet pipe and liquid discharge pipe to pass.
As the further improvement of technique scheme, the two ends of described culturing bottle are respectively equipped with bottle cap, and described each bottle cap is offered respectively porose, and described hole forms nutrient solution entrance and waste liquid outlet, and described semi-permeable membranes is arranged in bottle cap.
As the further improvement of technique scheme, described frame comprises the support of both sides, and the top of described support is provided with crossbeam, and two suspension ring are installed on described crossbeam, is arranged on respectively the two ends of two suspension rods connection cradles on two suspension ring.
The beneficial effects of the utility model are: the cradle nutrient solution that makes to be contained in culturing bottle that swings back and forth forms awave motion, cell and nutrient solution have been played to good oxygen supply and mixed effect, the system that the shearing force that this mode of motion produces is mixed much smaller than stirring-type, greatly reduce the damage of cell, culturing bottle is provided with nutrient solution entrance and waste liquid outlet simultaneously, fresh nutrient solution can constantly supplement the waste liquid that contains meta-bolites of discharging, make the growth of cell obtain enough nutrients, improved culture efficiency.
Accompanying drawing explanation
Below in conjunction with drawings and embodiments, the utility model is further illustrated.
Fig. 1 is frontview of the present utility model;
Fig. 2 is vertical view of the present utility model;
Fig. 3 is left view of the present utility model;
Fig. 4 is schematic diagram of the present utility model.
Embodiment
As shown in Figures 1 to 4, the extensive suspension cell culture device of swing type in the present embodiment, comprises frame and rack-mounted cradle 1.Frame is made up of the support 13 of both sides, the cross bar 19 that is threaded in two crossbeams 14 between support 13 tops and is arranged on one of them support 13 middle part.On crossbeam 14, be provided with on 15, two suspension ring 15 of two suspension ring suspension rod 16 is installed respectively, every suspension rod 16 is threaded in respectively the two ends of cradle 1, makes suspension ring 15 can be with cradle 1 to wave around the rotation of crossbeam 14.In cradle 1, be provided with cushion, place culturing bottle 3 on cushion, cell and nutrient solution can be injected in the inside of culturing bottle 3, therefore, culturing bottle 3 can, along with the waving and stable swing of cradle 1, allow cell suspension in culturing bottle 3, thereby form large-scale suspension culture.
An oscillating motor 2 is installed on cross bar 19, thereby this oscillating motor 2 is by driving suspension rod 16 band cradles 1 to swing.On oscillating motor 2, install and be provided with controller 5, this controller 5 is electrically connected with oscillating motor 2 by circuit, and then the rotating speed of control oscillating motor 2 is adjusted the swing rate of cradle 1.Meanwhile, controller 5 can also be controlled the amplitude of fluctuation of cradle 1.Therefore the present embodiment can replace the operating mode of artificial culture ware, has improved the efficiency of cell cultures and the survival rate of cell, and has reduced the pollution bringing due to artificial culture; In addition, because the size of culturing bottle 3 can arbitrarily regulate and control, so the present embodiment can meet the requirement that cell large scale is cultivated.
Preferably, the two ends of culturing bottle 3 are respectively equipped with bottle cap 12, and each bottle cap 12 inside are all installed and are provided with semi-permeable membranes 4, and each bottle cap 12 all offers a hole, forms respectively nutrient solution entrance and waste liquid outlet.The effect of semi-permeable membranes 4 is to allow nutrient solution by stoping cell to pass through, and guarantees that cell is always in culturing bottle 3 inside.Nutrient solution entrance is connected to the liquid-inlet pipe 6 that silica gel is made, and liquid-inlet pipe 6 is connected in the culture bag that fresh cell culture fluid is housed 17 of suspension through after the through hole of cradle 1 one end; Waste liquid outlet is connected to the liquid discharge pipe 7 that silica gel is made, and liquid discharge pipe 7 is connected on waste liquid bottle 18 through after the through hole of cradle 1 the other end.Thus, the nutrient solution in culturing bottle 3 is able to continuous replacing, has guaranteed the culture effect of cell.
For the amount that nutrient solution is flowed into and flow out keeps basically identical, on liquid-inlet pipe 6 and liquid discharge pipe 7, automatically controlled shut off valve and under meter are installed respectively, each shut off valve, under meter are electrically connected with controller 5 respectively.For instance, as shown in Figure 4, when work, shut off valve 8 on liquid discharge pipe 7 is opened, in the time that the under meter 9 on liquid discharge pipe 7 detects effluent discharge 50mL, controller 5 controls shut off valve 8 and cuts out, and the shut off valve 8 ' of liquid-inlet pipe 6 is opened, flow into 50mL until the under meter 9 ' of liquid-inlet pipe 6 detects nutrient solution, now close shut off valve 8 '; Until after setting-up time, shut off valve 8 is opened, so circulation.
In the present embodiment, controller 5, except controlling the keying of rotating speed, amplitude of fluctuation, shut off valve of oscillating motor 2, is also provided with safety switch, and abnormal rear automatic shutdown circuit appears in whole device.
Preferably, being connected to air intake branch 10 on liquid-inlet pipe 6, being provided with waterproof ventilated membrane 11 in air intake branch 10, make outside air along with liquid-inlet pipe 6 enters culturing bottle 3, is nutrient solution and cell supplemental oxygen.
The present embodiment uses and is achieved in that before experiment, be ready to treat cultured cells and fresh nutrient solution, be injected in culturing bottle 3 with syringe holder cell and nutrient solution, culturing bottle 3 is installed in cradle 1, liquid-inlet pipe 6 and liquid discharge pipe 7 are by through hole and extraneous UNICOM on cradle 1, liquid-inlet pipe 6 one ends are connected in the culture bag that fresh cell culture fluid is housed 17 of suspension, liquid discharge pipe 7 is connected on the waste liquid bottle 18 of splendid attire waste liquid, start two-position controller 5, regulate the amplitude of fluctuation of oscillating motor 2 rotating speeds and cradle 1, what make that cell can be uniform and stable is suspended in culturing bottle 3, controlled the keying of shut off valve by two-position controller 5, guarantee that the discharge of waste liquid and the influx of nutrient solution are basically identical, cell is in a best growing environment forever, the surviving rate of cell is improved, the efficiency of culturing cell increases.
The above be the utility model preferred embodiment, it does not form the restriction to the utility model protection domain.
Claims (8)
1. the extensive suspension cell culture device of swing type, it is characterized in that: comprise frame and rack-mounted cradle (1), in described frame, be provided with the oscillating motor (2) for driving cradle (1) to swing, in described cradle (1), be mounted with culturing bottle (3), described culturing bottle (3) is provided with nutrient solution entrance and waste liquid outlet, and described nutrient solution entrance and waste liquid outlet place are provided with semi-permeable membranes (4).
2. the extensive suspension cell culture device of swing type according to claim 1, is characterized in that: in described oscillating motor (2) or frame, be provided with the controller (5) of adjusting oscillating motor (2) rotating speed.
3. the extensive suspension cell culture device of swing type according to claim 2, it is characterized in that: described nutrient solution entrance is connected to liquid-inlet pipe (6), described waste liquid outlet is connected to liquid discharge pipe (7), on described liquid-inlet pipe (6) and liquid discharge pipe (7), shut off valve is all installed, described each shut off valve is electrically connected with controller (5).
4. the extensive suspension cell culture device of swing type according to claim 3, it is characterized in that: on described liquid-inlet pipe (6) and liquid discharge pipe (7), under meter is all installed, described each under meter is electrically connected with controller (5).
5. according to the extensive suspension cell culture device of the swing type described in claim 3 or 4, it is characterized in that: on described liquid-inlet pipe (6), be connected to air intake branch (10), in described air intake branch (10), be provided with waterproof ventilated membrane (11).
6. the extensive suspension cell culture device of swing type according to claim 3, is characterized in that: described cradle (1) two ends are respectively equipped with the through hole that supplies liquid-inlet pipe (6) and liquid discharge pipe (7) to pass.
7. the extensive suspension cell culture device of swing type according to claim 1, it is characterized in that: the two ends of described culturing bottle (3) are respectively equipped with bottle cap (12), described each bottle cap (12) is offered respectively porose, described hole forms nutrient solution entrance and waste liquid outlet, and described semi-permeable membranes (4) is arranged in bottle cap (12).
8. according to the extensive suspension cell culture device of the swing type described in claim 1 or 7, it is characterized in that: described frame comprises the support (13) of both sides, the top of described support (13) is provided with crossbeam (14), two suspension ring (15) are installed on described crossbeam (14), are arranged on respectively the two ends of two suspension rods (16) the connection cradles (1) on two suspension ring (15).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320659669.2U CN203625382U (en) | 2013-10-24 | 2013-10-24 | Oscillating large-scale suspension cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201320659669.2U CN203625382U (en) | 2013-10-24 | 2013-10-24 | Oscillating large-scale suspension cell culture device |
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| Publication Number | Publication Date |
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| CN203625382U true CN203625382U (en) | 2014-06-04 |
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| CN201320659669.2U Expired - Fee Related CN203625382U (en) | 2013-10-24 | 2013-10-24 | Oscillating large-scale suspension cell culture device |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296342A (en) * | 2015-11-30 | 2016-02-03 | 周伟萌 | Anaerobic treatment device |
| CN108641952A (en) * | 2018-05-07 | 2018-10-12 | 宁波华仪宁创智能科技有限公司 | The bionical culture apparatus of cell and method |
| CN109679849A (en) * | 2019-01-29 | 2019-04-26 | 四川省肿瘤医院 | A kind of Three-Dimensional Dynamic cell culture system |
| CN112457979A (en) * | 2020-11-19 | 2021-03-09 | 江西迈柯菲生物医药科技有限公司 | Double-cylinder centrifugal large-scale suspension type cell culture device |
-
2013
- 2013-10-24 CN CN201320659669.2U patent/CN203625382U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105296342A (en) * | 2015-11-30 | 2016-02-03 | 周伟萌 | Anaerobic treatment device |
| CN108641952A (en) * | 2018-05-07 | 2018-10-12 | 宁波华仪宁创智能科技有限公司 | The bionical culture apparatus of cell and method |
| CN109679849A (en) * | 2019-01-29 | 2019-04-26 | 四川省肿瘤医院 | A kind of Three-Dimensional Dynamic cell culture system |
| CN109679849B (en) * | 2019-01-29 | 2024-06-07 | 四川省肿瘤医院 | Three-dimensional dynamic cell culture system |
| CN112457979A (en) * | 2020-11-19 | 2021-03-09 | 江西迈柯菲生物医药科技有限公司 | Double-cylinder centrifugal large-scale suspension type cell culture device |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140604 Termination date: 20141024 |
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| EXPY | Termination of patent right or utility model |