CN204162717U - A kind of microorganism continuous culture device - Google Patents
A kind of microorganism continuous culture device Download PDFInfo
- Publication number
- CN204162717U CN204162717U CN201420616702.8U CN201420616702U CN204162717U CN 204162717 U CN204162717 U CN 204162717U CN 201420616702 U CN201420616702 U CN 201420616702U CN 204162717 U CN204162717 U CN 204162717U
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- China
- Prior art keywords
- culture tank
- controlling system
- nutritive medium
- microorganism
- liquid level
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Abstract
The utility model discloses a kind of microorganism continuous culture device, comprise nutritive medium storage bottle, Controlling System, culture tank, liquid level sensor, turbidity transducer and nutrient solution receiving flask, described nutritive medium storage bottle is connected with culture tank by feeding tube, feeding tube is provided with fresh feed pump, described fresh feed pump is connected with Controlling System, in described culture tank, liquid level sensor is installed, turbidity transducer and agitator, liquid level sensor is connected with Controlling System by wire with turbidity transducer, above culture tank, motor is installed, motor one end is connected with Controlling System, the motor the other end is connected with agitator, inlet pipe is provided with in described culture tank, inlet pipe one end is connected with air compressor, culture tank is connected with sampling unit, culture tank side is connected with nutrient solution receiving flask.The utility model makes the nutritive medium in culture tank with reasonable concentration, while realizing the cultured continuously of microorganism, ensures the reproduction speed of microorganism.
Description
[technical field]
The utility model relates to the technical field of microbial cultivation device, particularly a kind of technical field of microorganism continuous culture device.
[background technology]
The cultured continuously of microorganism is for batch culture.In a relatively independent airtight system, disposable input substratum is commonly referred to as batch culture to the mode that microorganism carries out inoculation culture.Due to the relative stopping property of its culture systems, therefore batch culture is also named airtight cultivation.Batch culture as the microorganism culturing of carrying out as culture vessel with flask in microbe research.Adopt this batch process, with the prolongation of incubation time, due to the relative stopping property of system, be can not get supplementing in time by the nutrition of microbial consumption, meta-bolites fails to discharge culture systems in time, other has inhibiting envrionment conditions to can not get timely improvement to microorganism growth, nutritional condition needed for growth of microbial cells breeding and outside atmosphere are progressively worsened, thus microbial population growth table is revealed progressively enter quick growth from cell to adapting to of new environment, then comparatively fast proceed to stationary phase, the population development that the stage finally moving towards to become feeble and die is clearly demarcated.
Cultured continuously refers on the basis of the inherent mechanism that growth curve is formed in further investigation batch culture, open culture system, the training method of continuous Additional nutrient solution, the derepression factor, Optimal Growing metabolism environment.Due to the relative openness of culture systems, therefore, cultured continuously is also referred to as open culture.Distinguishing feature and the advantage of cultured continuously are, it can according to the object of investigator, to a certain extent, certain period in manual control typical growth curve, make it to shorten or time expand, make the cell in certain period accelerate or reduce metabolic rate, thus greatly improve artificial controllability and the efficiency of culturing process.
[utility model content]
The purpose of this utility model solves the problems of the prior art exactly, a kind of microorganism continuous culture device is proposed, the supplementary supply of nutritive medium can be carried out to culture tank by nutritive medium storage bottle, liquid level sensor can detect the liquid level in culture tank, turbidity transducer can detect the turbidity of nutrient solution in culture tank, thus make Controlling System control the feed speed of the nutritive medium of fresh feed pump, make the nutritive medium in culture tank with reasonable concentration, while realizing the cultured continuously of microorganism, ensure the reproduction speed of microorganism.
For achieving the above object, the utility model proposes a kind of microorganism continuous culture device, comprise nutritive medium storage bottle, Controlling System, culture tank, liquid level sensor, turbidity transducer and nutrient solution receiving flask, described nutritive medium storage bottle is connected with culture tank by feeding tube, feeding tube is provided with fresh feed pump, described fresh feed pump is connected with Controlling System, in described culture tank, liquid level sensor is installed, turbidity transducer and agitator, liquid level sensor is connected with Controlling System by wire with turbidity transducer, above culture tank, motor is installed, motor one end is connected with Controlling System, the motor the other end is connected with agitator, inlet pipe is provided with in described culture tank, inlet pipe one end is connected with air compressor, culture tank is connected with sampling unit, culture tank side is connected with nutrient solution receiving flask.
As preferably, described nutritive medium storage bottle is provided with feed-pipe, and feed-pipe side is provided with voltage stabilizing tube, and nutrient solution receiving flask is also provided with voltage stabilizing tube, and described voltage stabilizing tube is provided with air filter.
As preferably, described feeding tube adopts silicone tube, and feeding tube and nutritive medium storage bottle connection are provided with sealing-ring, and feeding tube and culture tank junction are provided with dropper, described fresh feed pump employing peristaltic pump.
As preferably, described inlet pipe one end is positioned at the bottom of culture tank, and the other end of inlet pipe is connected with air compressor by air filter.
As preferably, described Controlling System is connected with supply unit, the end of agitator is provided with paddle.
The beneficial effects of the utility model: the utility model can carry out the supplementary supply of nutritive medium to culture tank by nutritive medium storage bottle, liquid level sensor can detect the liquid level in culture tank, turbidity transducer can detect the turbidity of nutrient solution in culture tank, thus make Controlling System control the feed speed of the nutritive medium of fresh feed pump, make the nutritive medium in culture tank with reasonable concentration, ensure the reproduction speed of microorganism.
Feature of the present utility model and advantage will be described in detail by reference to the accompanying drawings by embodiment.
[accompanying drawing explanation]
Fig. 1 is the front view of a kind of microorganism continuous culture device of the utility model.
[embodiment]
Consult Fig. 1, a kind of microorganism continuous culture device of the utility model, comprise nutritive medium storage bottle 1, Controlling System 2, culture tank 3, liquid level sensor 4, turbidity transducer 5 and nutrient solution receiving flask 6, described nutritive medium storage bottle 1 is connected with culture tank 3 by feeding tube 11, feeding tube 11 is provided with fresh feed pump 12, described fresh feed pump 12 is connected with Controlling System 2, in described culture tank 3, liquid level sensor 4 is installed, turbidity transducer 5 and agitator 7, liquid level sensor 4 is connected with Controlling System 2 by wire with turbidity transducer 5, motor 71 is installed above culture tank 3, motor 71 one end is connected with Controlling System 2, motor 71 the other end is connected with agitator 7, inlet pipe 8 is provided with in described culture tank 3, inlet pipe 8 one end is connected with air compressor 81, culture tank 3 is connected with sampling unit 31, culture tank 3 side is connected with nutrient solution receiving flask 6.
Described nutritive medium storage bottle 1 is provided with feed-pipe 13, feed-pipe 13 side is provided with voltage stabilizing tube 9, nutrient solution receiving flask 6 is also provided with voltage stabilizing tube 9, described voltage stabilizing tube 9 is provided with air filter 91, described feeding tube 11 adopts silicone tube, feeding tube 11 and nutritive medium storage bottle 1 connection are provided with sealing-ring 14, feeding tube 11 and culture tank 3 junction are provided with dropper 32, described fresh feed pump 12 adopts peristaltic pump, described inlet pipe 8 one end is positioned at the bottom of culture tank 3, the other end of inlet pipe 8 is connected with air compressor 81 by air filter 91, described Controlling System 2 is connected with supply unit 21, the end of agitator 7 is provided with paddle 72.
Utility model works process:
In a kind of microorganism continuous culture device busy of the utility model process, enough supplementary supplies being carried out nutritive medium by nutritive medium storage bottle 1 pair of culture tank 3, liquid level sensor 4 can detect the liquid level in culture tank 3, turbidity transducer 5 can detect the turbidity of nutrient solution in culture tank 3, liquid level sensor 4 and turbidity transducer 5 by signal feedback to Controlling System 2, thus make the feed speed of the nutritive medium of Controlling System control 2 fresh feed pump processed, make the nutritive medium in culture tank 3 with reasonable concentration, ensure the reproduction speed of microorganism.
Above-described embodiment is to explanation of the present utility model, is not to restriction of the present utility model, anyly all belongs to protection domain of the present utility model to the scheme after the utility model simple transformation.
Claims (5)
1. a microorganism continuous culture device, it is characterized in that: comprise nutritive medium storage bottle (1), Controlling System (2), culture tank (3), liquid level sensor (4), turbidity transducer (5) and nutrient solution receiving flask (6), described nutritive medium storage bottle (1) is connected with culture tank (3) by feeding tube (11), feeding tube (11) is provided with fresh feed pump (12), described fresh feed pump (12) is connected with Controlling System (2), liquid level sensor (4) is installed in described culture tank (3), turbidity transducer (5) and agitator (7), liquid level sensor (4) is connected with Controlling System (2) by wire with turbidity transducer (5), culture tank (3) top is provided with motor (71), motor (71) one end is connected with Controlling System (2), motor (71) the other end is connected with agitator (7), inlet pipe (8) is provided with in described culture tank (3), inlet pipe (8) one end is connected with air compressor (81), culture tank (3) is connected with sampling unit (31), culture tank (3) side is connected with nutrient solution receiving flask (6).
2. a kind of microorganism continuous culture device as claimed in claim 1, it is characterized in that: described nutritive medium storage bottle (1) is provided with feed-pipe (13), feed-pipe (13) side is provided with voltage stabilizing tube (9), nutrient solution receiving flask (6) is also provided with voltage stabilizing tube (9), described voltage stabilizing tube (9) is provided with air filter (91).
3. a kind of microorganism continuous culture device as claimed in claim 1, it is characterized in that: described feeding tube (11) adopts silicone tube, feeding tube (11) and nutritive medium storage bottle (1) connection are provided with sealing-ring (14), feeding tube (11) and culture tank (3) junction are provided with dropper (32), and described fresh feed pump (12) adopts peristaltic pump.
4. a kind of microorganism continuous culture device as claimed in claim 1, it is characterized in that: described inlet pipe (8) one end is positioned at the bottom of culture tank (3), the other end of inlet pipe (8) is connected with air compressor (81) by air filter (91).
5. a kind of microorganism continuous culture device according to any one of Claims 1-4, it is characterized in that: described Controlling System (2) is connected with supply unit (21), the end of agitator (7) is provided with paddle (72).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420616702.8U CN204162717U (en) | 2014-10-17 | 2014-10-17 | A kind of microorganism continuous culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420616702.8U CN204162717U (en) | 2014-10-17 | 2014-10-17 | A kind of microorganism continuous culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN204162717U true CN204162717U (en) | 2015-02-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420616702.8U Expired - Fee Related CN204162717U (en) | 2014-10-17 | 2014-10-17 | A kind of microorganism continuous culture device |
Country Status (1)
| Country | Link |
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| CN (1) | CN204162717U (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105420100A (en) * | 2016-01-07 | 2016-03-23 | 刘操 | Continuous and efficient culture device for probiotics |
| CN107475093A (en) * | 2017-07-10 | 2017-12-15 | 湖北物连网智能用品有限公司 | A kind of microorganism heating water bath culture apparatus |
| CN108456620A (en) * | 2018-01-17 | 2018-08-28 | 江苏联友科研仪器有限公司 | A kind of novel methane oxidation bacteria culture test system |
| CN109642195A (en) * | 2016-08-31 | 2019-04-16 | 通用电气健康护理生物科学股份公司 | The detection of foam levels |
| CN110423690A (en) * | 2019-08-08 | 2019-11-08 | 上海青赛生物科技有限公司 | A kind of cell factory pipe-line system |
-
2014
- 2014-10-17 CN CN201420616702.8U patent/CN204162717U/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105420100A (en) * | 2016-01-07 | 2016-03-23 | 刘操 | Continuous and efficient culture device for probiotics |
| CN109642195A (en) * | 2016-08-31 | 2019-04-16 | 通用电气健康护理生物科学股份公司 | The detection of foam levels |
| CN107475093A (en) * | 2017-07-10 | 2017-12-15 | 湖北物连网智能用品有限公司 | A kind of microorganism heating water bath culture apparatus |
| CN108456620A (en) * | 2018-01-17 | 2018-08-28 | 江苏联友科研仪器有限公司 | A kind of novel methane oxidation bacteria culture test system |
| CN110423690A (en) * | 2019-08-08 | 2019-11-08 | 上海青赛生物科技有限公司 | A kind of cell factory pipe-line system |
| CN110423690B (en) * | 2019-08-08 | 2022-08-30 | 上海青赛生物科技有限公司 | Cell factory pipeline system |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20150218 Termination date: 20151017 |
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| EXPY | Termination of patent right or utility model |