CN203708882U - Multifunctional liquid strain culturing device - Google Patents
Multifunctional liquid strain culturing device Download PDFInfo
- Publication number
- CN203708882U CN203708882U CN201420093794.6U CN201420093794U CN203708882U CN 203708882 U CN203708882 U CN 203708882U CN 201420093794 U CN201420093794 U CN 201420093794U CN 203708882 U CN203708882 U CN 203708882U
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- casing
- water bath
- wireway
- dividing plate
- blake bottle
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Abstract
The utility model relates to a multifunctional liquid strain culturing device, and belongs to the technical field of strain culturing. A partition board is arranged at the bottom of a tank and divides the tank into an upper layer and a lower layer; a heating device is arranged between the partition board and a bottom plate of the tank; water is injected into the tank, a water bath is formed, a temperature inductor is arranged in the water bath, culture bottles are placed in the water bath and fixed on the side wall of the tank through solid clips, and a gas-guide tube I and a gas-guide tube II extend into each culture bottle through a silica gel bottle stopper of the culture bottle. The multifunctional liquid strain culturing device can simultaneously culture multiple species of liquid strains, strain production can be finished for one time, and the infection risk of the liquid strains is effectively reduced; meanwhile, the device is provided with a plurality of inoculation points, and the inoculation speed and efficiency are increased; and the multifunctional liquid strain culturing device is low in cost, small in risk, simple in structure, convenient to operate, suitable for scale production and capable of effectively meeting the requirement of scale production of strains.
Description
Technical field
The utility model relates to a kind of Multifunctional liquid strain culture apparatus, belongs to breeding strain technical field.
Background technology
Along with going from strength to strength of China's mushroom industry, scale, standardization, standardized enterprise and cultivation base are on the increase, and the demand of low cost, low-risk, large-scale production bacterial classification is become clear day by day; Yet there is a lot of defects in production and the culture technology of conventional liquid spawn at present: the equipment such as the need of production culture tank of conventional liq bacterial classification, magnetic stirring apparatus, shaking table, equipment is many, and cost is high, and is difficult for promoting; Especially bacterial classification, from one-level kind to three grade kind, use the same method and expand numerous 3 times, and it is very risky that bacterial classification infects; Meanwhile, the production cycle of whole liquid spawn is long, needs 40 days, and the production cycle long, waste a large amount of manpowers and the energy, be incompatible with the demand of social development and production.
Therefore, necessary prior art is improved, to adapt to the demand of social development and production, promoted going from strength to strength of mushroom industry, to scale, standardization, Standardization Development.
utility model content
For the problems referred to above; the utility model is to provide a kind of Multifunctional liquid strain culture apparatus, can cultivate the liquid spawn of many kinds simultaneously, disposablely completes the production of hybrid seeds; effectively reduce the risk that liquid spawn infects; meanwhile, the utility model has a plurality of vaccinations, improves inoculation speed and efficiency; the utility model cost is low, risk is little, be applicable to large-scale production device; simple in structure, easy to operate, can effectively meet the demand of large-scale production bacterial classification.
For technical solution problem, the technical solution adopted in the utility model: described Multifunctional liquid strain culture apparatus comprises blake bottle 1, electronic temperature controller 2, temperature inductor 3, dividing plate 4, heater 5, casing 6, water bath 7, wireway I 8, wireway II 9, culture bag 10 and solid clip 11, casing 6 bottoms are provided with dividing plate 4, dividing plate 4 blow-by, dividing plate 4 is divided into casing 6 two-layer up and down, between the base plate of dividing plate 4 and casing 6, be provided with heater 5, the interior injected water of casing 6, form water bath 7, temperature inductor 3 is placed in water bath 7, be fixed on the sidewall of casing 6, the output of temperature inductor 3 is connected with electronic temperature controller 2, electronic temperature controller 2 is fixed on the outside of casing 6, the top blow-by of casing 6, blake bottle 1 is placed in water bath 7, by solid clip 11, be fixed on the sidewall of casing 6, the bottom of blake bottle 1 is placed on dividing plate 4, wireway I 8 and wireway II 9 stretch in blake bottle 1 by the silica gel bottle stopper of blake bottle 1, wherein, wireway I 8 stretches into culture fluid bottom, more than wireway II 9 is positioned at culture fluid liquid level.
The water temperature of described water bath 7 keeps 25 ℃.
Described heater 5 adopts heating tube.
Described casing 6 adopts stainless steel material to make.
Described blake bottle 1 is transparent vessel, and number is more than one.
Described blake bottle 1, electronic temperature controller 2, temperature inductor 3, dividing plate 4, heater 5, casing 6, water bath 7, wireway I 8, wireway II 9 and solid clip 10 all adopt commercially available common components.
The beneficial effects of the utility model: the utility model can be cultivated the liquid spawn of many kinds simultaneously; disposablely complete the production of hybrid seeds; effectively reduce the risk that liquid spawn infects; meanwhile, the utility model has a plurality of vaccinations, improves inoculation speed and efficiency; the utility model cost is low, risk is little, be applicable to large-scale production device; simple in structure, easy to operate, can effectively meet the demand of large-scale production bacterial classification.
Accompanying drawing explanation
Fig. 1 is structural representation of the present utility model;
Fig. 2 is schematic diagram before use of the present utility model;
Fig. 3 is schematic diagram in use of the present utility model;
In figure: 1-blake bottle, 2-electronic temperature controller, 3-temperature inductor, 4-dividing plate, 5-heater, 6-casing, 7-water bath, 8-wireway I, 9-wireway II, 10-solid clip.
Embodiment
Below in conjunction with accompanying drawing and implementation process, the utility model is described further, to facilitate the technical staff to understand.
As shown in Figure 1: described Multifunctional liquid strain culture apparatus comprises blake bottle 1, electronic temperature controller 2, temperature inductor 3, dividing plate 4, heater 5, casing 6, water bath 7, wireway I 8, wireway II 9, culture bag 10 and solid clip 11, casing 6 bottoms are provided with dividing plate 4, dividing plate 4 blow-by, dividing plate 4 is divided into casing 6 two-layer up and down, between the base plate of dividing plate 4 and casing 6, be provided with heater 5, the interior injected water of casing 6, form water bath 7, temperature inductor 3 is placed in water bath 7, be fixed on the sidewall of casing 6, the output of temperature inductor 3 is connected with electronic temperature controller 2, electronic temperature controller 2 is fixed on the outside of casing 6, the top blow-by of casing 6, blake bottle 1 is placed in water bath 7, by solid clip 11, be fixed on the sidewall of casing 6, the bottom of blake bottle 1 is placed on dividing plate 4, to strengthen the stability of blake bottle 1, wireway I 8 and wireway II 9 stretch in blake bottle 1 by the silica gel bottle stopper of blake bottle 1, wherein, wireway I 8 stretches into culture fluid bottom, more than wireway II 9 is positioned at culture fluid liquid level.
Described heater 5 can adopt common heating tube.
Described blake bottle 1, electronic temperature controller 2, temperature inductor 3, dividing plate 4, heater 5, casing 6, water bath 7, wireway I 8, wireway II 9 and solid clip 10 all adopt commercially available common components.
The course of work of the present utility model:
As shown in Figure 2: first, to the interior injected water of water bath 7, start heater 5, temperature inductor 3 is inputted temperature signal in electronic temperature controller 2, by electronic temperature controller 2, water temperature in water bath 7 is controlled to 25 ℃; Culture fluid is put into blake bottle 1, with silica gel bottle stopper, obturage, then, wireway I 8 connects oxygen system, in the air input blake bottle 1 purifying, more than wireway II 9 is positioned at culture fluid liquid level, by the cause Bas Discharged in blake bottle 1, after treating the cause Bas Discharged in blake bottle 1, as shown in Figure 3, wireway II 9 is connected with oxygen system, for the bacterial classification of cultivating provides sufficient oxygen, until the liquid spawn of 1 li, blake bottle makes, last, tapping bacterial classification from wireway I 8.
Liquid spawn is to adopt biological culture (fermentation) equipment, and the mode of (fermentation) of cultivating by deep liquid is produced edible fungi ball, as the seed of edible fungus culturing.Its liquid spawn is the physical behavior of relative solid spawn (wheat, boll hull, wheat bran, branch etc.).Liquid spawn has the incomparable advantage of solid spawn.
As preferred version, described casing 6 adopts stainless steel material to make, blake bottle 1 is transparent vessel, number is more than one, utilize the bull oxygen hose of oxygen system to be connected with blake bottle 1 respectively, each blake bottle 1 is a vaccination, realize one group of multiple spot classification inoculation apparatus, effectively improve inoculation speed and efficiency, the liquid spawn that operator can observe blake bottle 1 from casing 6 tops is cultivating process, the vigor that can clearly see bacterial classification changes and growth conditions, effectively control quality and the growth rate of liquid spawn, whole incubation can be observed by artificial, timely discovery situation, cultivation is polluted to be effectively controlled.
The utility model can also be cultivated in same production technology for the liquid spawn of many kinds, once can cultivate two kinds of above liquid spawns simultaneously, disposablely completes the production of hybrid seeds, and cultivation cycle is short, can effectively guarantee quality and the purity of bacterial classification.
The utility model can meet the growth demand of thalline to environment such as nutrition, temperature, oxygen, acid-base values to greatest extent, and the metabolism waste gas that respiration produces can be got rid of in time, metabolism is vigorous, and all bacterium coccuses of Peloton density are basically identical age, so mycelia is energetic.And equipment is in the past because incubation time is long, often, cause the cell age time long, admittedly affect spawn activity.
The utility model is without shaking table and oscillator, reduce investment, reduce incubation times, shortened incubation time, reduced pollution rate, larger reduction the production cost of bacterial classification and cultivation management, meanwhile, its manufacture craft is simple, culture efficiency is high, and infection rate is low, can produce in batches, practical.
The utility model describes by accompanying drawing, in the situation that not departing from the utility model scope, can also carry out various conversion and be equal to replacement the utility model patent, therefore, the utility model patent is not limited to disclosed specific implementation process, and should comprise the whole embodiments that fall within the scope of the utility model Patent right requirement.
Claims (5)
1. a Multifunctional liquid strain culture apparatus, it is characterized in that: described Multifunctional liquid strain culture apparatus comprises blake bottle, electronic temperature controller, temperature inductor, dividing plate, heater, casing, water bath, wireway I, wireway II, culture bag and solid clip, bottom half is provided with dividing plate, dividing plate is two-layer up and down for casing is divided into, dividing plate blow-by, between dividing plate and the base plate of casing, be provided with heater, injected water in casing, form water bath, temperature sense is placed in water bath, be fixed on the sidewall of casing, the output of temperature inductor is connected with electronic temperature controller, electronic temperature controller is fixed on the outside of casing, the top blow-by of casing, blake bottle is placed in water bath, by solid clip, be fixed on the sidewall of casing, the bottom of blake bottle is placed on dividing plate, wireway I and wireway II stretch in blake bottle by the silica gel bottle stopper of blake bottle, wherein, wireway I stretches into culture fluid bottom, more than wireway II is positioned at culture fluid liquid level.
2. a kind of Multifunctional liquid strain culture apparatus as claimed in claim 1, is characterized in that: the water temperature of described water bath keeps 25 ℃.
3. a kind of Multifunctional liquid strain culture apparatus as claimed in claim 1, is characterized in that: described heater adopts heating tube.
4. a kind of Multifunctional liquid strain culture apparatus as claimed in claim 1, is characterized in that: described casing adopts stainless steel material to make.
5. a kind of Multifunctional liquid strain culture apparatus as claimed in claim 1, is characterized in that: described blake bottle is transparent vessel, and number is more than one.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN201420093794.6U CN203708882U (en) | 2014-03-04 | 2014-03-04 | Multifunctional liquid strain culturing device |
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CN201420093794.6U CN203708882U (en) | 2014-03-04 | 2014-03-04 | Multifunctional liquid strain culturing device |
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CN203708882U true CN203708882U (en) | 2014-07-16 |
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CN201420093794.6U Expired - Fee Related CN203708882U (en) | 2014-03-04 | 2014-03-04 | Multifunctional liquid strain culturing device |
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Cited By (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106489533A (en) * | 2016-11-30 | 2017-03-15 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris winter cultivating technique |
CN106718031A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris hot weather cultivating technique |
CN106718035A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of winter current temperature control Cordyceps militaris culture pond |
CN106718039A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of summer current temperature control Cordyceps militaris culture pond |
CN107746805A (en) * | 2017-11-17 | 2018-03-02 | 江南大学 | A kind of method of Liquid static culture edible and medicinal fungi |
CN111296185A (en) * | 2020-04-03 | 2020-06-19 | 河北民族师范学院 | Domestic multi-variety multi-stubble multi-point positioning edible mushroom fruiting device and fruiting method |
-
2014
- 2014-03-04 CN CN201420093794.6U patent/CN203708882U/en not_active Expired - Fee Related
Cited By (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106489533A (en) * | 2016-11-30 | 2017-03-15 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris winter cultivating technique |
CN106718031A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of Cordyceps militaris hot weather cultivating technique |
CN106718035A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of winter current temperature control Cordyceps militaris culture pond |
CN106718039A (en) * | 2016-11-30 | 2017-05-31 | 张家港市藏联生物研究所有限公司 | A kind of summer current temperature control Cordyceps militaris culture pond |
CN107746805A (en) * | 2017-11-17 | 2018-03-02 | 江南大学 | A kind of method of Liquid static culture edible and medicinal fungi |
CN107746805B (en) * | 2017-11-17 | 2019-04-23 | 江南大学 | A kind of method of Liquid static culture edible and medicinal fungi |
CN111296185A (en) * | 2020-04-03 | 2020-06-19 | 河北民族师范学院 | Domestic multi-variety multi-stubble multi-point positioning edible mushroom fruiting device and fruiting method |
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C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
CF01 | Termination of patent right due to non-payment of annual fee | ||
CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140716 |