CN202898398U - Photobioreactor for culturing and collecting microalgae - Google Patents

Photobioreactor for culturing and collecting microalgae Download PDF

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Publication number
CN202898398U
CN202898398U CN 201220592057 CN201220592057U CN202898398U CN 202898398 U CN202898398 U CN 202898398U CN 201220592057 CN201220592057 CN 201220592057 CN 201220592057 U CN201220592057 U CN 201220592057U CN 202898398 U CN202898398 U CN 202898398U
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China
Prior art keywords
reactor
cone
microalgae
support frame
little algae
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Expired - Fee Related
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CN 201220592057
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Chinese (zh)
Inventor
裴海燕
韩飞
胡文容
祁峰
宋明明
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Shandong University
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Shandong University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/20Degassing; Venting; Bubble traps
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/22Settling tanks; Sedimentation by gravity
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

Abstract

The utility model belongs to the technical field of microalgae biological energy technology, and relates to a photobioreactor for culturing and collecting microalgae, and the photobioreactor comprises a reactor main body, a top cover, a bottom cone, a temperature controller, a charging hole, an air inlet hole, an exhaust pipe, a sampling hole, a liquid outlet, a valve, a rubber sealing ring, an aeration device, an internal support frame and an external support frame, wherein a reactor cone is slidably connected with the reactor main body by a flange. The photobioreactor for culturing and collecting the microalgae can be used for realizing the natural sedimentation and collection of the microalgae and reducing the later centrifugal energy consumption. Furthermore, the photobioreactor is capable of automatically adjusting the environment temperature of microalgae growth in a reactor, so that the microalgae can be effectively guaranteed to grow and propagate at the optimum temperature. The reactor is internally provided with the aeration device, so that the algae liquid in the whole reactor can be well mixed, and the sedimentation and adherence of the growing period microalgae can be avoided; and good gas exchange can be realized, so that the culture efficiency is greatly improved.

Description

The bioreactor that a kind of little algae is cultivated and collects
Technical field
The utility model belongs to little algae bioenergy research field, relate to a kind of little algae can automatically regulate in cultivating algae liquid temp, gaseous interchange fully, increase microalgae harvesting, reduce the bioreactor of centrifugal energy consumption, be used for that little algae is cultivated and the development of relevant little algae bioenergy.
Background technology
The fossil energy in the world consumes fast now, and carbonic acid gas discharges in a large number, and Greenhouse effect are sought the discharging that the new continuable renewable energy resources can effectively reduce carbonic acid gas in the atmosphere in continuous aggravation.Little algae is a kind of the growth rapidly and the extremely strong biology of environmental compatibility, and very large trophology, pharmacy and biological value are arranged, and can be used as the raw material of some biology and pharmaceutical industries, is used for production high added value nutritious prod, biofuel and fish food.With respect to other biological matter, utilize little algae production biofuel, can greatly reduce floor space, enhance productivity, save time, therefore little algae is a kind of biomass that have DEVELOPMENT PROSPECT, the utility model can be used for little algae R and D.
Produce little algal biomass and generally adopt artificial culture at present in a large number, comprise openly and closed, open mainly is open raceway pond, and closed mainly is bioreactor.Open micro algae culturing device is fairly simple, initial cost is little, convenient management, shortcoming be little algae of cultivating easily contaminated, algae cell density is low, floor space is large etc., production efficiency is low in practice owing to this mode, directly production and the development research of the little algae of impact, therefore this training method is used few.Closed photo bioreactor can effectively stop the invasion of external contaminant, and the cultivation management is more convenient, and algae cell density is high, and production efficiency is high, regulates and control easily, has therefore obtained using more widely, and its shortcoming is that initial cost is large, and reactor cleans difficulty.And after utilizing the reactor results algae liquid of above two kinds of forms, by methods such as centrifugal, flocculations algae liquid is processed again, obtained the little biomass of water ratio, adopt this method power consumption large, and time loss is many, has limited taking full advantage of of closed photo bioreactor.The temperature of algae liquid can only rely on the external world to come indirectly control in the ordinary light bio-reactor, and not only energy consumption is larger, and in the optimum growth temperature scope that maintains little algae that can not be stable, is unfavorable for little algae Fast Growth.
Summary of the invention
The purpose of this utility model is to overcome the shortcoming that the popular response device exists, design a kind of automatic adjusting algae liquid temp, gaseous interchange fully, increase microalgae harvesting, reduce the reactor assembly of centrifugal energy consumption.
For achieving the above object, the technical scheme taked of the utility model is:
The utility model comprises reactor body, top cover, bottom cone, temperature regulator, charging opening, inlet mouth, vapor pipe, thief hole, leakage fluid dram, valve, rubber seal, aerating apparatus, inner support frame and outer support frame, this reactor bottom is cone structure, the cone point is positioned on the reactor vertical centering control axis, reactor body is tightly connected by flange and bottom cone, is provided with leakage fluid dram in the sharp position of cone.The aeration opening of aerating apparatus is connected by plastics tubing with the inlet mouth of reactor body lower end.On the reactor body inwall automatic temperature control device is housed.The bottom surface radius of bottom cone is identical with reactor cylinder-shaped body radius, and the base angle of bottom cone is in 55-60 degree scope, and the drift angle of cone is in 70-60 degree scope.The lower end of reactor body and outer support frame bind, and the bottom cone of reactor is provided with bracing frame.
Little algae in the utility model comprises algae and salt water algae, such as: chlorella, four tail grid algaes, scenedesmus obliquus, chrysophyceae, Phaeodactylum tricornutum, diatom etc. can be used for little algae and cultivate the development that reaches relevant little algae bioenergy.
Reactor material of the present utility model is synthetic glass, and preferably light transmission is arranged.
Material of the present utility model mainly contains nutrient solution and algae liquid, filtrated air and compression arbon dioxide, and the medicine that is used for sterilization.
Advantage of the present utility model:
This bioreactor adopts cone structure, can collect the high density algae liquid after the frustule natural subsidence, reduces the centrifugal energy consumption of later stage algae liquid; The reactor cone base is equipped with aerating apparatus, can play good algae liquid and mix, replenishes inorganic carbon source and remove the effect that oxygen is closed in satiety, prevents the frustule adherent growth, alleviates the murder by poisoning that supersaturation oxygen produces frustule; Inside reactor is equipped with Automatic Temperature Control, is used for regulating the algae liquid temp, is conducive to little algae and grows under optimum temperuture; Design of the present utility model has improved little algae culture efficiency greatly, for the further little algae Resource Supply of development research possibility.
Description of drawings
Fig. 1 is the utility model cross-sectional view;
Fig. 2 is the utility model tangent plane structural representation;
1 is vapor pipe, and 2 is the charging opening rubber plug, and 3 is top cover, 4 is rubber ring, and 5 is flange bolt, and 6 is charging opening, 7 is Automatic Temperature Control, and 8 is valve, and 9 is thief hole, 10 is reactor body, and 11 is the aeration inlet mouth, and 12 is outer support frame, 13 is the reactor cone, 14 is inner support frame, and 15 is aerating apparatus, and 16 is leakage fluid dram.
Embodiment
Shown in Fig. 1-2, the utility model is the optical-biological reaction apparatus that a kind of little algae is cultivated and collects.This device comprises vapor pipe 1, charging opening rubber plug 2, top cover 3, rubber ring 4, flange bolt 5, charging opening 6, Automatic Temperature Control 7, valve 8, thief hole 9, reactor body 10, aeration inlet mouth 11, outer support frame 12, bottom cone 13, inner support frame 14, aerating apparatus 15, leakage fluid dram 16.
The cone 13 of reactor bottom connects inner support frame 14, has leakage fluid dram 16 at the cone apex angle place, at reactor bottom aerating apparatus 15 is housed, and links to each other with inlet pipe 11, and whole cone is connected by flange seal with reactor body; The reactor body lower end is connected with outer support frame 12, up evenly have successively four thief holes 9 from the bottom in cylinder one side, thief hole is by valve 8 control foldings, and Automatic Temperature Control 7 is equipped with in opposite side inwall middle part, and the cylinder top is tightly connected by flange and reactor head 3; 1, one liquid charging opening 6 of vapor pipe that bends to 90 degree is arranged on the top cover, and with rubber plug 2 jam-pack charging openings, reactor body 10 connects as one by flange bolt 5 and top cover 3 and bottom cone 13 during the reactor operation.
Reactor material is synthetic glass, reactor vapor pipe 1 internal diameter is 7mm, and charging opening 6 diameters are 1cm, reactor body 10 high 25-50cm, thief hole 9 internal diameters are 7mm, tapered section 13 high 5-11cm, leakage fluid dram 16 internal diameters are 1cm, the bottom surface radius of reverse bottom cone is identical with the reactor body radius, be 6-12cm, the bottom of interior outer support frame of supporting whole reactor is in the same plane, and wherein outer support frame 12 highly is 8-14cm, and inner support frame 14 highly is 5-8cm.
Connect the flange of reactor cone 13 and reactor body 10 during use, close all valves 8, connect aerating apparatus 15, connect source of the gas, in reactor, add algae liquid and nutrient solution, extremely liquid level is 27-57cm in the whole reactor, connect the flange of reactor head, filled in the rubber plug 2 at charging opening 6 places, regulate temperature regulator 7 to the optimum temperuture of little algae of cultivating, open the valve at inlet mouth 11 places, simultaneously with the certain flow aeration, begin to cultivate little algae, gas is discharged from vapor pipe 1; After the culture cycle end, the valve of closing inlet mouth 11 places treats that the frustule natural subsidence is concentrated, opens the valve at reactor conical lower portion leakage fluid dram 16 places, results high density algae liquid.Behind the results high density algae liquid, in reactor, add new nutrient solution by charging opening 6, repeat aforesaid operations, realize semicontinuous or cultured continuously.

Claims (5)

1. bioreactor that little algae is cultivated and collects, comprise reactor body, top cover, bottom cone, temperature regulator, charging opening, inlet mouth, vapor pipe, thief hole, leakage fluid dram, valve, rubber seal, aerating apparatus, inner support frame and outer support frame, it is characterized in that this reactor bottom is cone structure, the cone point is positioned on the reactor vertical centering control axis, reactor body is tightly connected by flange and bottom cone, is provided with leakage fluid dram in the sharp position of cone.
2. the bioreactor of a kind of little algae cultivation according to claim 1 and collection is characterized in that the aeration opening of described aerating apparatus is connected by plastics tubing with the inlet mouth of reactor body lower end.
3. the bioreactor of a kind of little algae cultivation according to claim 1 and collection is characterized in that on the reactor body inwall automatic temperature control device being housed.
4. a kind of little algae according to claim 1 bioreactor of cultivating and collecting, the bottom surface radius that it is characterized in that bottom cone is identical with reactor cylinder-shaped body radius, the base angle of bottom cone is in 55-60 degree scope, and the drift angle of cone is in 70-60 degree scope.
5. the bioreactor of a kind of little algae cultivation according to claim 1 and collection is characterized in that the lower end of reactor body and outer support frame bind, and the bottom cone of reactor is provided with inner support frame.
CN 201220592057 2012-11-09 2012-11-09 Photobioreactor for culturing and collecting microalgae Expired - Fee Related CN202898398U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201220592057 CN202898398U (en) 2012-11-09 2012-11-09 Photobioreactor for culturing and collecting microalgae

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201220592057 CN202898398U (en) 2012-11-09 2012-11-09 Photobioreactor for culturing and collecting microalgae

Publications (1)

Publication Number Publication Date
CN202898398U true CN202898398U (en) 2013-04-24

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Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104130929A (en) * 2013-05-02 2014-11-05 台美生物资源股份有限公司 Multi-module circulating-type microalgae breeding system having energy-saving function
CN104962455A (en) * 2015-06-24 2015-10-07 中山安荞生物科技有限公司 Illumination bioreactor
CN106281974A (en) * 2016-10-28 2017-01-04 天津大学 A kind of oil-producing microalgae culture apparatus and cultural method thereof
CN110564585A (en) * 2019-08-28 2019-12-13 孙瑗 Closed type photo-biological reaction tank

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104130929A (en) * 2013-05-02 2014-11-05 台美生物资源股份有限公司 Multi-module circulating-type microalgae breeding system having energy-saving function
CN104962455A (en) * 2015-06-24 2015-10-07 中山安荞生物科技有限公司 Illumination bioreactor
CN106281974A (en) * 2016-10-28 2017-01-04 天津大学 A kind of oil-producing microalgae culture apparatus and cultural method thereof
CN110564585A (en) * 2019-08-28 2019-12-13 孙瑗 Closed type photo-biological reaction tank

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20130424

Termination date: 20151109

EXPY Termination of patent right or utility model