CN106281974A - A kind of oil-producing microalgae culture apparatus and cultural method thereof - Google Patents
A kind of oil-producing microalgae culture apparatus and cultural method thereof Download PDFInfo
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- CN106281974A CN106281974A CN201610966808.4A CN201610966808A CN106281974A CN 106281974 A CN106281974 A CN 106281974A CN 201610966808 A CN201610966808 A CN 201610966808A CN 106281974 A CN106281974 A CN 106281974A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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Abstract
The invention discloses a kind of oil-producing microalgae culture apparatus and cultural method thereof, device includes metal rack, culture tank base, culture tank and culture tank top cover it is sequentially installed with from top to bottom on metal rack, the top of culture tank and bottom are respectively arranged with drain hole I and drain hole II, culture tank top cover is provided with sample tap and injection port, and the side of culture tank is provided with light source, and opposite side is provided with air pump, air pump is provided with the airway stretched into bottom culture tank, and the end of airway is provided with air distribution head.Cultural method comprises the following steps: algae kind and culture fluid mixed liquor are added in culture tank by (1), and keeping initial absorbance value is 0.1；(2) culture apparatus is placed in thermostatic chamber, set environment temperature；(3) culture apparatus position is moved, it is ensured that at its axis, illumination value is 2500Lx；(4) regulation air pump flow, arranges light cycle；(5), after cultivating a period of time continuously, obtain the final absorbance of micro algae culturing liquid and be converted into microalgae dry weight.
Description
Technical field
The invention belongs to biodiesel technology field, particularly relate to one and produce oil-producing microalgae Biomass simultaneously and locate simultaneously
The micro algae culturing device of reason sewage and cultural method thereof.
Background technology
With microalgae for raw material production biodiesel owing to yield per unit area is high, the generation time is short, resource requirement is little, can hold
Continue the reasons such as property is strong and receive significant attention.But, how to reduce higher production cost and be always microalgae biodiesel field
Research emphasis.Wherein, as the important component part of production cost, microdisk electrode also achieves weight under research in recent years
Big progress.
Cultivate microalgae to produce the culture medium of biodiesel and generally utilize pure water as culture medium, and need in incubation
Nutrient substance to be added.A large amount of consumption of pure water and nutrient substance are the main sources of toxigenic capacity.In order to reduce this cost,
Research worker proposes to utilize sewage as culture medium culturing microalgae, and reaches oil-producing and processes the purpose of sewage simultaneously.Although at present
There are some devices for microdisk electrode, but are substantially applicable to microalgae pure culture, control strict, operate complicated.For
Sewage cultivation is optimized and micro algae culturing device simple to operate easy to control is the most less.
Summary of the invention
The invention aims to overcome deficiency of the prior art, it is provided that a kind of compact conformation, easy to control, production
Efficiency is high, practicality wide, can quantity-produced micro algae culturing device and cultural method thereof.
It is an object of the invention to be achieved through the following technical solutions:
A kind of oil-producing microalgae culture apparatus, is made up of device body, including metal rack, on described metal rack from lower to
On be sequentially installed with culture tank base, culture tank and culture tank top cover, the top of described culture tank and bottom are respectively arranged with puts
Material mouth I and drain hole II, described culture tank top cover is provided with sample tap and injection port, and the side of described culture tank is provided with light source,
Opposite side is provided with air pump, and described air pump is provided with the airway stretched into bottom culture tank, and the end of described airway is provided with gas distribution
Head, described airway is additionally provided with effusion meter；It is additionally provided with guide shell in described culture tank.
Described culture tank and described culture tank base and the separable installation of culture tank top cover, it is simple to dismount, clean and disappear
Poison.
It is equipped with control valve on described injection port, drain hole I and drain hole II.
The cultural method of a kind of oil-producing microalgae culture apparatus, comprises the following steps:
(1) adding in culture tank by algae kind and culture fluid mixed liquor, keeping initial absorbance value is 0.1；
(2) culture apparatus is placed in thermostatic chamber, set environment temperature；
(3) culture apparatus position is moved, it is ensured that at its axis, illumination value is 2500Lx；
(4) regulation air pump flow, arranges light cycle；
(5), after cultivating a period of time continuously, obtain the final absorbance of micro algae culturing liquid and be converted into microalgae dry weight.
Described algae kind is at least one in chlorella, diatom, cyanophyceae.
Compared with prior art, technical scheme is had the benefit that
1. the main body of culture tank and culture tank base and culture tank top cover achieve separation, it is simple to dismount, clean, disappear
Poison.
2. air pump is provided with the airway stretched into bottom culture tank, can be passed through bottom culture tank by gas, can be by providing
Carbon dioxide or air are to strengthen Biomass and the disposal ability thereof of oil-producing microalgae.
3. the end of airway is provided with air distribution head, can fully mix culture fluid, prevent frustule from settling.
4. it is provided with guide shell, the flowing of bootable culture fluid in culture tank, effectively prevents aeration from causing the sinuous flow of culture fluid.
5. it is provided with charging aperture and drain hole, can be used in series, be suitable for fairly large use.
Accompanying drawing explanation
Fig. 1 is the structural representation of culture apparatus of the present invention.
Reference: 1-culture tank, 2-culture tank top cover, 3-culture tank base, 4-guide shell, 5-sample tap, 6-gas distribution
Head, 7-metal rack, 8-injection port, 9-drain hole I, 10-drain hole II, 11-light source, 12-air pump, 13-effusion meter 14-leads
Trachea
Detailed description of the invention
The invention will be further described below in conjunction with the accompanying drawings:
As it is shown in figure 1, a kind of oil-producing microalgae culture apparatus, it is made up of device body, including metal rack 7, metal rack 7
On be sequentially installed with culture tank base 3, culture tank 1 and culture tank top cover 2 from top to bottom, the top of culture tank 1 and bottom are respectively
Being provided with drain hole I9 and drain hole II10, culture tank top cover 2 is provided with sample tap 5 and injection port 8, and the side of culture tank 1 sets
Having light source 11, opposite side is provided with air pump 12, and air pump 12 is provided with the airway 14 stretched into bottom culture tank 1, the end of airway 14
End is provided with air distribution head 6, and air distribution head 6 can be additionally provided with effusion meter 13 on bottom even aeration, airway 14；Leading in culture tank 1
Guide shell 4 it is additionally provided with, in order to guide culture fluid to flow around trachea.In addition on injection port 8, drain hole I9 and drain hole II10 all
It is provided with control valve.
By the cultural method of the present invention a kind of oil-producing microalgae culture apparatus, carry out the training of following embodiment altogether
Support:
Embodiment 1
A kind of method that oil-producing microalgae is cultivated, comprises the steps:
Use the oil-producing microalgae culture apparatus shown in Fig. 1,
(1) pure strain (Chlorella vulgaris) and sewage mixed liquor culture medium mixed liquor are added in culture tank,
Keeping initial absorbance value is 0.1；
(2) being placed in thermostatic chamber by reactor, set environment temperature is 28 DEG C；
(3) mobile reactor position, it is ensured that at its axis, illumination value is 2500Lx；
(4) regulation air pump flow is 20L min-1, every 12h aeration 5min；
(5) after cultivating 12d continuously, the final absorbance obtaining micro algae culturing liquid is 1.836, closes microalgae dry weight
0.718g·L-1。
Embodiment 2
A kind of method that oil-producing microalgae is cultivated, comprises the steps:
Use the oil-producing microalgae culture apparatus shown in Fig. 1,
(1) pure strain (Scenedesmusobliquus) is added in culture tank with sewage mixed liquor, keep initial extinction
Angle value is 0.1；
(2) being placed in thermostatic chamber by reactor, set environment temperature is 28 DEG C；
(3) mobile reactor position, it is ensured that at its axis, illumination value is 2500Lx；
(4) regulation air pump flow is 20L min-1, every 12h aeration 5min；
(5) after cultivating 12d continuously, the final absorbance obtaining micro algae culturing liquid is 1.332, closes microalgae dry weight
0.519g·L-1。
Embodiment 3
A kind of method that oil-producing microalgae is cultivated, comprises the steps:
Use the oil-producing microalgae culture apparatus shown in Fig. 1,
(1) mixed algae kind (Chlorella vulgaris:Scenedesmusobliquus=1:1) is mixed with sewage
Liquid adds in culture tank, and keeping initial absorbance value is 0.1；
(2) being placed in thermostatic chamber by reactor, set environment temperature is 28 DEG C；
(3) mobile reactor position, it is ensured that at its axis, illumination value is 2500Lx；
(4) regulation air pump flow is 20L min-1, every 12h aeration 5min；
(5) after cultivating 12d continuously, the final absorbance obtaining micro algae culturing liquid is 1.770, closes microalgae dry weight
0.692g·L-1。
Embodiment 4
A kind of method that oil-producing microalgae is cultivated, comprises the steps:
Use the oil-producing microalgae culture apparatus shown in Fig. 1,
(1) mixed algae kind (Chlorella vulgaris:Scenedesmus obliquus=1:1) is mixed with sewage
Liquid adds in culture tank, and keeping initial absorbance value is 0.1；
(2) being placed in thermostatic chamber by reactor, set environment temperature is 28 DEG C；
(3) mobile reactor position, it is ensured that at its axis, illumination value is 2500Lx；
(4) regulation air pump flow is 4L min-1, continue aeration；
(5) after cultivating 12d continuously, the final absorbance obtaining micro algae culturing liquid is 1.806, closes microalgae dry weight
0.706g·L-1。
Embodiment 5
A kind of method that oil-producing microalgae is cultivated, comprises the steps:
Use the oil-producing microalgae culture apparatus shown in Fig. 1,
(1) mixed algae kind (Chlorella vulgaris:Scenedesmus obliquus=1:1) is mixed with sewage
Liquid adds in culture tank, and keeping initial absorbance value is 0.1；
(2) being placed in thermostatic chamber by reactor, set environment temperature is 28 DEG C；
(3) mobile reactor position, it is ensured that at its axis, illumination value is 2500Lx；
(4) regulation air pump flow is 4L min-1, continue aeration；
(5) cultivating 60d continuously, after 14d, the absorbance obtaining micro algae culturing liquid is stable 1.974 ± 0.267,
Close microalgae dry weight 0.773 ± 0.096g L-1。
Embodiment 6
A kind of method that oil-producing microalgae is cultivated, comprises the steps:
Use the oil-producing microalgae culture apparatus shown in Fig. 1,
(1) mixed algae kind (Chlorella vulgaris:Scenedesmus obliquus=1:1) is mixed with sewage
Liquid adds in 5 foster tanks that training is in parallel, and all keeping initial absorbance value is 0.1；
(2) being placed in thermostatic chamber by reactor, set environment temperature is 28 DEG C；
(3) mobile reactor position, it is ensured that at its axis, illumination value is 2500Lx；
(4) realizing air pump simultaneously aeration in 5 culture tank by air valve, regulation air pump flow is 20L min-1, continue
Aeration；
(5) cultivating 60d continuously, after 15d, the absorbance of the micro algae culturing liquid of 5 culture tank is stablized respectively
1.803 ± 0.189,1.725 ± 0.252,2.058 ± 0.165,1.938 ± 0.117 and 1.881 ± 0.201, close microalgae respectively
Dry weight 0.705 ± 0.065mg L-1, 0.674 ± 0.095mg L-1, 0.806 ± 0.056mg L-1, 0.759 ±
0.037mg·L-1With 0.736 ± 0.070mg L-1。
The present invention is not limited to embodiments described above.Description to detailed description of the invention is intended to describe and say above
Bright technical scheme, above-mentioned detailed description of the invention is only schematically, is not restrictive.Without departing from this
In the case of invention objective and scope of the claimed protection, those of ordinary skill in the art also may be used under the enlightenment of the present invention
Make the concrete conversion of a lot of form, within these belong to protection scope of the present invention.
Claims (5)
1. an oil-producing microalgae culture apparatus, is made up of device body, it is characterised in that include that metal rack, described metal prop up
Being sequentially installed with culture tank base, culture tank and culture tank top cover on frame from top to bottom, top and the bottom of described culture tank are divided
Not being provided with drain hole I and drain hole II, described culture tank top cover is provided with sample tap and injection port, the side of described culture tank
Being provided with light source, opposite side is provided with air pump, and described air pump is provided with the airway stretched into bottom culture tank, the end of described airway
It is provided with air distribution head, described airway is additionally provided with effusion meter；It is additionally provided with guide shell in described culture tank.
A kind of oil-producing microalgae culture apparatus, it is characterised in that described culture tank and described culture tank
Base and the separable installation of culture tank top cover, it is simple to dismount, clean and sterilize.
A kind of oil-producing microalgae culture apparatus, it is characterised in that described injection port, drain hole I and put
It is equipped with control valve on material mouth II.
The cultural method of a kind of oil-producing microalgae culture apparatus, it is characterised in that include following step
Rapid:
(1) adding in culture tank by algae kind and culture fluid mixed liquor, keeping initial absorbance value is 0.1；
(2) culture apparatus is placed in thermostatic chamber, set environment temperature；
(3) culture apparatus position is moved, it is ensured that at its axis, illumination value is 2500Lx；
(4) regulation air pump flow, arranges light cycle；
(5), after cultivating a period of time continuously, obtain the final absorbance of micro algae culturing liquid and be converted into microalgae dry weight.
The cultural method of a kind of oil-producing microalgae culture apparatus the most according to claim 4, it is characterised in that described algae kind is
At least one in chlorella, diatom, cyanophyceae.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365691A (en) * | 2017-08-28 | 2017-11-21 | 厦门大学 | A kind of micro algae culturing device and method of controllable biological concentration |
| CN108102879A (en) * | 2018-01-23 | 2018-06-01 | 河海大学 | A kind of separator of Microcystis aeruginosa morphologic species and the separation method of Microcystis aeruginosa morphologic species |
| CN116463197A (en) * | 2023-06-19 | 2023-07-21 | 愚公生态科技股份有限公司 | Carbon fixation photobioreactor and application thereof |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107365691A (en) * | 2017-08-28 | 2017-11-21 | 厦门大学 | A kind of micro algae culturing device and method of controllable biological concentration |
| CN107365691B (en) * | 2017-08-28 | 2021-03-09 | 厦门大学 | Microalgae culture device and method capable of controlling biological concentration |
| CN108102879A (en) * | 2018-01-23 | 2018-06-01 | 河海大学 | A kind of separator of Microcystis aeruginosa morphologic species and the separation method of Microcystis aeruginosa morphologic species |
| CN108102879B (en) * | 2018-01-23 | 2019-07-16 | 河海大学 | A kind of separator of Microcystis aeruginosa morphologic species and the separation method of Microcystis aeruginosa morphologic species |
| CN116463197A (en) * | 2023-06-19 | 2023-07-21 | 愚公生态科技股份有限公司 | Carbon fixation photobioreactor and application thereof |
| CN116463197B (en) * | 2023-06-19 | 2024-02-02 | 愚公生态科技股份有限公司 | Carbon fixation photobioreactor and application thereof |
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