CN101985595B - Membrane type photobioreactor for enhancing microalgae culturing - Google Patents
Membrane type photobioreactor for enhancing microalgae culturing Download PDFInfo
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- 239000012528 membrane Substances 0.000 title claims abstract description 70
- 238000012258 culturing Methods 0.000 title abstract description 6
- 230000002708 enhancing effect Effects 0.000 title abstract 3
- 241000195493 Cryptophyta Species 0.000 claims description 69
- 238000012546 transfer Methods 0.000 claims description 19
- 230000002787 reinforcement Effects 0.000 claims description 13
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 21
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Abstract
The invention discloses a membrane type photobioreactor for enhancing microalgae culturing. The membrane type photobioreactor comprises an external light source, a bottom plate, an inner sleeve and an outer sleeve, wherein the inner sleeve and the outer sleeve are vertically arranged on the bottom plate; the outer sleeve is positioned on the outer side of the inner sleeve and surrounds the inner sleeve; the top end of the outer sleeve is horizontally provided with a transparent top cover plate, the top of the outer sleeve is provided with a plurality of air outlets and the bottom of the outer sleeve is provided with a discharge port; the bottom of the inner sleeve is provided with a plurality of exchange holes; the bottom plate is provided with an air inlet which is surrounded by the inner sleeve; the air outlets are positioned above the top end of the inner sleeve; the bottom plate is vertically provided with a membrane component which is positioned inside the inner sleeve; and the bottom edge of the membrane component is hermetically fixed with the bottom plate and surrounds the air inlet. The membrane type photobioreactor for enhancing microalgae culturing has the advantages of simple structure, simplicity and convenience in operation, stable performance, larger specific surface area and light transmission efficiency; and by using the membrane component, the gas is uniformly distributed, and the photobioreactor can be independently used or connected with a track pool for amplified culturing of the microalgae.
Description
Technical field
The present invention relates to algae culture reactor technology field, especially relate to a kind of membrane type bioreactor that little algae is cultivated of strengthening.
Background technology
Little algae be a class on land, the ocean is widely distributed, the high holophyte of nutritious, photosynthetic availability, the polysaccharide that cellular metabolism produces, protein, pigment etc. make it have good DEVELOPMENT PROSPECT in fields such as food, medicine, genetically engineered, liquid fuels.The advantages such as little algae is expected to crack a key of energy dilemma as rear petroleum times, have production capacity large, and is pollution-free, renewable, development prospect is very fine.But due to the research of the little algae of the domestic energy and use and still be in the starting stage, still have many problems to be difficult to solve, and for the research and development of optical-biological reaction apparatus required in little algae culturing process, also be absolutely necessary.
The purpose of design of the reactor of algae culture is to pursue larger light-receiving area, eliminates the oxygen accumulation.The reactor of algae culture mainly contains two kinds of open and closeds.Open type biological reactor is larger owing to being affected by the external environment, and is not efficient manner; Compare with the open reactive device, closed has and is easy to guarantee gnotobasis, is easy to control culture condition, be easy to realize the advantages such as high-density culture, therefore at present the Closed photobioreactor kind is many, uses also more extensively, mainly contains several forms such as tubular type, flat, pillar.
The bubble tower type bioreactor is that a platform is arranged on the reactor base plate, around platform, guard post and light source are arranged, light source all is connected with time controller, and the reactor tank body is located on platform, tank base has a temperature control interlayer, and air intake duct and carbon dioxide pipe enter respectively in gas container; The mixed gas escape pipe stretches out with strainer from the distribution container and is connected, and is connected with the gas distributor that is positioned at tank base by inlet pipe, and higher light-receiving area/volume ratio is arranged, and is difficult for producing the oxygen accumulation.
Patent publication No. CN101613664A discloses a kind of airlift photobioreactor, comprise the inner light source device in the reactor tank body, inoculation mouth and discharge gate on the reactor tank body wall, and the gauge hatch in the capping at reactor tank body top, lower capping and the temperature controller of venting port and bottom, dispenser device on lower capping central through hole, and rely on wherein that a plurality of distribution passages communicate with the inner cavity chamber of reactor tank body, nutrient substance is entered in the distribution passage by material hole through feeding tube respectively.This invention has realized the uniformly distributing of multimedium in reactor and has mixed, and is applicable to laboratory or industrial scale both culturing microalgae.
Bubbling style and airlift photobioreactor are two class reactors the most frequently used in the airtight cultivation of little algae.Because their simple in structure, easy handlings, and mixture strength and mass-transfer efficiency all higher, fluid type is very clear and definite.But two kinds of reactors are compared, and air lift type is more suitable for little algae and cultivates, because cell density skewness phenomenon can appear in bubbling style reactor in the axial direction, easily cause part microalgae cell hunger even dead.Strengthen membrane type bioreactor that little algae cultivates and be on the airlift reactor basis and improve, ring-type or crosswise porous gas sparger are replaced with the dead end hollow-fibre membrane.This improvement has effectively reduced bubble diameter, has enlarged the gas-to-liquid contact area, thereby has improved the gas-liquid mass transfer efficient of inside reactor, is conducive to lower concentration CO
2In time be supplied to frustule, and with the O that produces
2In time remove, avoid the photorespiration phenomenon to produce.
We in early stage studies show that (Fan LH, Zhang YT, Cheng LH, Zhang L, TangDS, Chen HL, Optimization of carbon dioxide fixation by Chlorelia vulgariscultivated in a membrane-photobioreactor, Chemical Engineering ﹠amp; Technology, 2007,30 (8): 1094-1099): membrane bioreactor can improve the rate of mass transfer of carbon dioxide and oxygen greatly, when cell density 15 * 10
7Individual/mL, induction air flow ratio 1.25L/min, red inner light source density 10800 luxs, the length 45cm of film, temperature is in 25 degrees centigrade, and the membrane bioreactor stabilizing carbon dioxide transforms oxygen and exceeds respectively 0.95 times and 0.86 times than common bio-reactor.Carbonic acid gas is the crystallized ability in bio-reactor and air inlet by algae, and illumination and membrane module have much relations.
Summary of the invention
The invention provides a kind of simple in structure, easy and simple to handle, stable performance is provided, have the membrane type bioreactor of cultivating than the little algae of the reinforcement of bigger serface and light transfer efficiency, this reactor adopts membrane module, make gas distribution even, can use separately, perhaps be used in conjunction for little algae amplification culture with raceway pond.
A kind of membrane type bioreactor of strengthening little algae cultivation comprises vertically disposed inner sleeve and outer sleeve on outer light source, base plate and base plate; Described outer sleeve is positioned at the outside of inner sleeve and inner sleeve is surrounded; The outer sleeve top is provided with horizontally disposed transparent lamina tecti, and the top is provided with some air outlets, and the bottom is provided with discharge port; Described inner sleeve bottom is provided with some exchange holes, described base plate is provided with the inlet mouth that is surrounded by inner sleeve, wherein, described air outlet is positioned at top, inner sleeve top, described base plate is provided with and is positioned at the membrane module that the inner sleeve inboard, upright arranges, and the bottom margin of membrane module and base plate sealing are fixed and inlet mouth is surrounded.
In order to satisfy the needs of multiple occasion, can be provided with the opening for feed that is surrounded by inner sleeve on base plate, opening for feed is connected with the feed-pipe of outside, and feed-pipe is provided with trip switch; The exchange hole outside is provided with latch plate, and the inner sleeve outer wall of exchange hole downside and both sides is provided with the chute that coordinates with latch plate, and latch plate can slide up and down along the both sides chute, and when moving on latch plate, exchange hole is open-minded, exchange hole sealing when sliding to bottom land under latch plate.When the membrane type bioreactor of the little algae cultivation of above-mentioned reinforcement uses separately, moving on latch plate, inner sleeve and outer sleeve are interconnected by exchange hole.During operation, close feed-pipe and discharge nozzle valve, ventilated by inlet mouth, utilize gas expansion to provide energy drives liquid to circulate, gas enters membrane module, disperses to enter algae liquid from fenestra, extend air or carbon dioxide gas mixture and liquid phase duration of contact, promote the absorption of carbonic acid gas, effectively improve the utilization ratio of carbonic acid gas, reduce the inhibition of the oxygen accumulation cell growth of photosynthesis generation.The excessive outer sleeve that enters of inner sleeve algae liquid, under action of gravity in outer sleeve algae liquid along inner sleeve wall to dirty, by movable span, enter again inner sleeve in the bottom, thereby realize circulation between inside and outside sleeve at inside reactor.When the membrane type bioreactor of the little algae cultivation of above-mentioned reinforcement is used for outdoor amplification occasion, general and raceway pond is used in conjunction, when being used in conjunction, latch plate is moved down exchange hole is sealed, separate inside and outside sleeve, algae liquid enters reactor by opening for feed by raceway pond, and inlet mouth connects membrane module and constantly bloats bubble, makes inner sleeve algae liquid be spilled over to outer sleeve, overflow algae liquid and be circulated to raceway pond by discharge port, effectively amplified the culture area of algae liquid.
Membrane module can be selected the cylindrical membrane assembly, and the cylindrical membrane assembly is comprised of the airtight hollow-fibre membrane of vertically disposed top film port, and the top height of membrane module is lower than the top height of inner sleeve.Membrane module also can be selected other shapes that can realize said function, can adjust according to actual needs.Adopt membrane module top film port airtight, prevent that gas from directly discharging from the film port, guarantee that gas only passes through from fenestra, fully disperse gas, gas contact with algae liquid more fully, effectively anti-block accumulates.Experiment showed, with the air-blowing of film port and compare, the fenestra air-blowing can significantly improve gas-liquid mass transfer efficient, and when the total area of film constant, when increasing the tubular fibre film length, be conducive to gas-liquid mixed and improve the carbon dioxide fixation rate.
Inner sleeve and outer sleeve generally adopt the transparent organic glass material, the light transmission that tool is good and wear resistance.Outer sleeve and inner sleeve are fixed by flange and base plate respectively, for ease of the fixing flange face that projection can be set in position corresponding to base plate.For preventing algae liquid seepage in the reactor operation, be provided with rubber cradle or O type circle with screw between flange bottom and base plate, the end face of installation rubber cradle during with the rubber cradle of screw and the ring seal striped that the bottom surface is provided with projection, the bottom surface of flange and the end face of base plate are respectively equipped with and the annular seal groove that seals striped and match.The number of sealing striped can be determined according to actual stopping property needs.Base plate requires the tool certain thickness, can support the weight of inside and outside sleeve, algae liquid and membrane module.
Lamina tecti is transparent material, generally selects transparent pmma material, can accept natural lighting; Outer light source is comprised of the fluorescent tube bulb of some vertical settings, and fluorescent tube bulb is evenly distributed on the surrounding of outer sleeve and fixes by support.The number of luminescent lamp, length and installation site are generally determined according to actual needs, for example, can adopt the luminescent lamp of 4～8 30W vertically to be distributed in reactor outward flange 10cm place, guarantee can obtain uniform illumination at reactor in all directions.
The air outlet outside is provided with baffle plate, and the top of baffle plate and outer sleeve outer wall are fixed, and turns up in the baffle plate bottom, and the bottom edge of baffle plate is lower than the bottom edge of air outlet, can prevent effectively that the foreign matter such as extraneous dust from entering internal system.The number of air outlet can be determined according to actual needs, generally is evenly distributed on the top of outer sleeve.The shape of air outlet also can be determined according to actual needs, without strict requirement.
Discharge port is connected with external discharge nozzle, and discharge nozzle is provided with thief hole, and thief hole is connected with external stopple coupon, and the thief hole diameter is less than the discharge port diameter.Reactor isolated operation or when being used in conjunction with raceway pond after processing for some time, when needing the experimental data of testing inner algae liquid, can be opened thief hole, and the algae liquid sample that takes a morsel is tested.For ease of the conveying of algae liquid generally opening for feed be set to equate with the diameter of discharge port, be generally 15～30mm; The diameter of thief hole is generally 5～10mm.
Inlet mouth is circular port, the sealing of inlet mouth place is fixed with transfer tube, the bottom equal diameters of transfer tube top internal diameter and membrane module and mutual sealing are fixing, transfer tube the other end diameter is fixed less than its top end diameter and with external inlet pipe, and the gas that so more is conducive to input by inlet mouth enters in the fenestra of membrane module more uniformly.In actual application, general direct transfer tube and base plate are tightly connected by rubber seal, and then the tip edge of membrane module bottom and transfer tube is fixed by bolt seal.
For the ease of the circulation of algae liquid between outer sleeve and inner sleeve, generally the difference of altitude between outer sleeve and inner sleeve top is set as 30～50cm.The too little algae liquid that easily causes of difference of altitude leaks out outer sleeve by the air outlet, and causes the loss of algae liquid; Difference of altitude causes too greatly the waste of material of outer sleeve, increases the manufacturing cost of reactor.
Beneficial effect of the present invention is embodied in:
(1) employing of membrane module in the present invention has improved the carbon dioxide fixation ability of membrane type bioreactor, and the contact area between gas phase and solid phase is increased, and has improved gas-liquid mass transfer speed; Membrane module top film port is airtight, prevents that gas from directly discharging from the film port, guarantees that gas only passes through from fenestra, fully disperses gas, makes gas more fully contact algae liquid, effectively anti-block accumulation;
(2) the membrane type bioreactor of the little algae cultivation of the reinforcement in the present invention, simple in structure, convenient operation, and range of application is wider, can use separately, also can be used for the little algae amplification culture that is used in conjunction with raceway pond.
Description of drawings
Fig. 1 is the structural representation of the membrane type bioreactor of the little algae cultivation of reinforcement of the present invention;
Fig. 2 is the partial enlarged drawing of strengthening the exchange hole place of membrane type bioreactor that little algae cultivates in Fig. 1;
Fig. 3 is the partial enlarged drawing of strengthening the C place of membrane type bioreactor that little algae cultivates in Fig. 1;
Fig. 4 is the vertical view of the rubber cradle of the membrane type bioreactor that in Fig. 1, the little algae of reinforcement is cultivated;
Fig. 5 is the sectional view along the A-A face of strengthening the membrane type bioreactor of little algae cultivation in Fig. 5;
Fig. 6 is the vertical view of the base plate of the membrane type bioreactor that in Fig. 1, the little algae of reinforcement is cultivated;
Fig. 7 is the sectional view along B-B of strengthening the membrane type bioreactor of little algae cultivation in Fig. 6;
Fig. 8 be strengthen in Fig. 1 membrane type bioreactor that little algae cultivates be used for being communicated with raceway pond the time structural representation.
Embodiment
As depicted in figs. 1 and 2, a kind of strengthen that little algae cultivates the membrane type bioreactor, comprise: the inner sleeve 4 and the outer sleeve 3 that vertically arrange on outer light source 2, horizontally disposed base plate 8 and base plate 8, for the ease of the circulation of algae liquid between outer sleeve 3 and inner sleeve 4, the difference of altitude between outer sleeve 3 and inner sleeve 4 tops is set as 40cm.Outer sleeve 3 is positioned at the outside of inner sleeve 4 and inner sleeve 4 is surrounded, outer sleeve 3 tops are provided with horizontally disposed transparent lamina tecti 1, outer sleeve 3 tops are provided with air outlet 20, air outlet 20 is positioned at the top on inner sleeve 4 tops, 20 outsides, air outlet are provided with baffle plate 12, outer sleeve 3 bottoms are provided with discharge port, and discharge port is connected with external discharge nozzle 7, and discharge nozzle 7 is provided with stopple coupon 6; Inner sleeve 4 bottoms are provided with exchange hole 14, and exchange hole 14 outsides are provided with latch plate 10, and base plate 8 is provided with inlet mouth 9 and the opening for feed that is surrounded by inner sleeve 4, and opening for feed is connected with extraneous feed-pipe 13, and base plate 8 vertically is provided with the membrane module 5 that is positioned at inner sleeve 4 inboards.
Outer light source 2 adopts the luminescent lamp of 8 30W vertically to be distributed in reactor outward flange 10cm place and utilizes support 18 fixing, guarantees can obtain uniform illumination at reactor in all directions.The specification of luminescent lamp and number can be adjusted according to actual needs again.
As shown in Fig. 3～7, outer sleeve 3 and inner sleeve 4 utilize bolt 11 fixing with base plate 8 by flange 16 respectively, for ease of fixing, the flange face of projection are set in the position of base plate 8 correspondences, are used for flange 16 fixing.For preventing algae liquid seepage in the reactor operation, be provided with the rubber cradle 15 with screw 24 between flange 16 bottom surfaces and base plate 8, the end face of rubber cradle 15 and bottom surface are provided with the ring seal striped 25 of projection, as shown in Figure 4 and Figure 5.The end face of the bottom surface of flange 16 and base plate 8 is respectively equipped with and the annular seal groove 26 that seals striped 25 and match.The number of sealing striped 25 can be definite according to actual stopping property needs, and base plate requires the tool certain thickness, can support the weight of inside and outside sleeve, algae liquid and membrane module, as shown in Figure 6 and Figure 7.
As shown in Figure 1, air outlet 20 on outer sleeve 3 is four square openings that size is 50mm * 50mm, be evenly distributed on outer sleeve 3 tops, 20 outsides, air outlet are provided with baffle plate 12, the top of baffle plate 12 and outer sleeve 3 are fixing, baffle plate 12 bottoms 45 degree that turn up, the bottom edge of baffle plate 12 is lower than the bottom edge of air outlet, can prevent effectively that the foreign matter such as extraneous dust from entering internal system.The number of air outlet 20 and shape also can be adjusted according to actual needs.The discharge port of outer sleeve 3 bottoms is connected with external discharge nozzle 7, and discharge nozzle 7 diameters are 20mm, and discharge nozzle 7 is provided with thief hole, and thief hole is connected with external stopple coupon 6, and the stopple coupon diameter is 10mm.During the reactor operation, after processing for some time, when needing the experimental data of testing inner algae liquid, can open stopple coupon 6, the algae liquid sample that takes a morsel is tested.
As shown in Figure 1, inlet mouth 9 on base plate is trapezoidal circular port, inlet mouth 9 places sealing is fixed with transfer tube 21, the bottom equal diameters of transfer tube 21 tops and membrane module 5 and interfixing by bolt seal, transfer tube 21 seals fixing with inlet mouth 9 by glass cement, transfer tube 21 the other end diameters are fixed less than its top end diameter and with external inlet pipe, so more are conducive to enter more uniformly in the fenestra of membrane module 5 by the gas of inlet mouth 9 inputs.
Membrane module 5 is cylindrical, is comprised of the airtight hollow-fibre membrane of vertically disposed top film port, and the top height of membrane module 5 is lower than the top height of inner sleeve 4.Adopt membrane module 5 top film ports airtight, prevent that gas from directly discharging from hollow hole, guarantee that gas only passes through from fenestra, can fully disperse gas, make gas more fully contact algae liquid, effectively anti-block accumulation.
As Fig. 1, shown in Figure 2, the exchange hole 14 that it is 40mm that inner sleeve 4 bottom evens are provided with 4 diameters, exchange hole 14 outsides are provided with square latch plate 10, and the area of latch plate 10 is greater than the area of exchange hole, and the inner sleeve outer wall of exchange hole 14 downsides and both sides is provided with the chute that coordinates with latch plate 10, latch plate 10 can slide up and down along the both sides chute, when moving on latch plate 10, exchange hole 14 is open-minded, and when latch plate 10 was displaced downwardly to bottom land, exchange hole 14 was sealed.
when the membrane type bioreactor of the little algae cultivation of above-mentioned reinforcement uses separately, moving on latch plate 10, inner sleeve 4 and outer sleeve 3 are interconnected by exchange hole 14, during operation, close the valve of feed-pipe 13 and discharge nozzle 7, by inlet mouth 9 ventilations, utilize gas expansion to provide energy drives liquid to circulate, gas enters membrane module 5, disperse to enter algae liquid from fenestra, extend air or carbon dioxide gas mixture and liquid phase duration of contact, promote the absorption of carbonic acid gas, effectively improve the utilization ratio of carbonic acid gas, reduce the inhibition of the oxygen accumulation cell growth of photosynthesis generation, the excessive outer sleeve 3 that enters of inner sleeve 4 algae liquid, algae liquid is along inner sleeve wall to dirty under action of gravity, pass through movable span 14 in the bottom, enter again inner sleeve 4, thereby realize circulation between inside and outside sleeve at inside reactor.
As shown in Figure 8, when the membrane type bioreactor of the little algae cultivation of above-mentioned reinforcement is used for outdoor amplification occasion, general and raceway pond 17 is used in conjunction, when being used in conjunction, latch plate 10 moves to the chute bottom land with exchange hole 14 sealings, separate inside and outside sleeve, algae liquid in raceway pond is transported to feed-pipe 13 by recycle pump 22, enter reactor by feed-pipe 13 by raceway pond 17, inlet mouth 9 connects membrane module 5 and constantly bloats bubble, make inner sleeve 4 algae liquid be spilled over to outer sleeve 3, overflow algae liquid and be circulated to raceway pond by discharge nozzle 7, effectively amplified the culture area of algae liquid;
For fully mixing of the algae liquid that keeps raceway pond, impeller 23 that can mounted motor drives in raceway pond.In raceway pond, algae liquid flow direction as shown in 8, takes back roadside siphunculus 24 and forms shunting with feed-pipe 13 on recycle pump 22, by regulating the variable valve on bypass pipe 24, can regulate the flow of the algae liquid that enters reactor.Through overregulating, make the flow of feed-pipe 13 identical with the flow of discharge nozzle 7, reach the purpose of mass flow balance in reactor.
Claims (5)
1. strengthen the membrane type bioreactor that little algae is cultivated for one kind, comprise vertically disposed inner sleeve and outer sleeve on outer light source, base plate and base plate; Described outer sleeve top is provided with horizontally disposed transparent lamina tecti, and the top is provided with some air outlets, and the bottom is provided with discharge port; Described inner sleeve bottom is provided with some exchange holes; Described base plate is provided with the inlet mouth that is surrounded by inner sleeve, it is characterized in that, described air outlet is positioned at top, inner sleeve top; Described base plate is provided with the membrane module of the vertical setting that is positioned at the inner sleeve inboard, and the bottom margin of membrane module and base plate sealing are fixing and inlet mouth is surrounded; Described base plate is provided with the opening for feed that is surrounded by inner sleeve, and opening for feed is connected with the feed-pipe of outside, and feed-pipe is provided with trip switch; The described exchange hole outside is provided with latch plate, and the inner sleeve outer wall of exchange hole downside and both sides is provided with the chute that coordinates with latch plate, and latch plate can slide up and down along the both sides chute;
Described membrane module is cylindrical, is comprised of the vertically disposed top airtight hollow-fibre membrane of fenestra, and the top height of membrane module is lower than the top height of inner sleeve;
Described inlet mouth is circular port, and the sealing of inlet mouth place is fixed with transfer tube, the bottom equal diameters of transfer tube top internal diameter and membrane module and mutually sealing fix, transfer tube bottom diameter is fixed less than its top end diameter and with external inlet pipe; Transfer tube downside and base plate are fixed;
The outside, described air outlet is provided with baffle plate, and the top of baffle plate and outer sleeve outer wall are fixed and higher than the air outlet height, the baffle plate bottom turn up and bottom edge lower than the bottom edge of air outlet;
Described outer sleeve and inner sleeve are fixed by flange and base plate respectively, be provided with the rubber cradle with screw between flange bottom and base plate, the end face of rubber cradle and bottom surface are provided with the ring seal striped of projection, and the bottom surface of flange and the end face of base plate are respectively equipped with and the annular seal groove that seals striped and match.
2. the membrane type bioreactor of the little algae cultivation of reinforcement according to claim 1, is characterized in that, described outer light source is comprised of the fluorescent tube bulb of some vertical settings, and fluorescent tube bulb is evenly distributed on the surrounding of outer sleeve and fixes by support.
3. the membrane type bioreactor cultivated of the little algae of reinforcement according to claim 1, it is characterized in that, described discharge port is connected with external discharge nozzle, and discharge nozzle is provided with thief hole, thief hole is connected with external stopple coupon, and the thief hole diameter is less than the discharge port diameter.
4. the membrane type bioreactor of the little algae cultivation of reinforcement according to claim 3, is characterized in that, the equal diameters of described opening for feed and discharge port is 15～30mm; Described thief hole diameter is 5～10mm.
5. the membrane type bioreactor cultivated of the little algae of reinforcement according to claim 1, is characterized in that, the difference of altitude between described outer sleeve and inner sleeve top is 30～50cm.
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Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102382755B (en) * | 2011-07-28 | 2013-09-18 | 中国科学院青岛生物能源与过程研究所 | Microalgae culturing device and microalgae culturing method |
| CN102304462B (en) * | 2011-08-29 | 2013-06-05 | 暨南大学 | Raceway pond photobiological reactor for implementing microalgae large-scale culture |
| CN102586078A (en) * | 2012-01-18 | 2012-07-18 | 杭州鑫伟低碳技术研发有限公司 | Microalgae cultivation system and method |
| CN102776120B (en) * | 2012-08-03 | 2013-10-16 | 北京理工大学 | Self-circulating microorganism culture device with oxygen provided by hollow fiber membrane, and culture method |
| CN102816689B (en) * | 2012-08-03 | 2013-10-16 | 北京理工大学 | Device and method for flat membrane oxygen-supply self-circulation-type microbial culture |
| CN102899239B (en) * | 2012-09-28 | 2014-02-26 | 重庆大学 | Dispersion optical-fiber type photo-bioreactor with inserted spiral band |
| CN104774750A (en) * | 2015-04-24 | 2015-07-15 | 亿利资源集团有限公司 | Photoreactor for culturing microalgae |
| GB2539936A (en) * | 2015-07-01 | 2017-01-04 | Univ Nelson Mandela Metropolitan | Microalgae cultivation process and equipment |
| CN105505756A (en) * | 2015-12-24 | 2016-04-20 | 青岛理工大学 | Breeding device for microalgae with high CO2 tolerance and fixation rate |
| CN105961303B (en) * | 2016-06-03 | 2019-02-12 | 山东大学 | A kind of ecological cultivation system and its operation method of fish-bacterium-algae symbiosis |
| CN115159771B (en) * | 2022-06-16 | 2024-02-27 | 科盛环保科技股份有限公司 | Water environment restoration device and technology |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101613664A (en) * | 2009-07-16 | 2009-12-30 | 青岛生物能源与过程研究所 | A kind of airlift photobioreactor |
-
2010
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101613664A (en) * | 2009-07-16 | 2009-12-30 | 青岛生物能源与过程研究所 | A kind of airlift photobioreactor |
Non-Patent Citations (3)
| Title |
|---|
| 微藻固定CO2研究进展;程丽华等;《生物工程学报》;20050331;第21卷(第2期);177-181 * |
| 程丽华等.微藻固定CO2研究进展.《生物工程学报》.2005,第21卷(第2期),177-181. |
| 范立海等.微藻膜式光生物反应器脱除CO2的研究.《第三届全国化学工程于生物化工年会论文摘要集》.2006,817. * |
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