CN203238266U - Photobioreactor device for culturing microalgae in high density - Google Patents
Photobioreactor device for culturing microalgae in high density Download PDFInfo
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- CN203238266U CN203238266U CN 201320254359 CN201320254359U CN203238266U CN 203238266 U CN203238266 U CN 203238266U CN 201320254359 CN201320254359 CN 201320254359 CN 201320254359 U CN201320254359 U CN 201320254359U CN 203238266 U CN203238266 U CN 203238266U
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- gas
- bioreactor
- photobioreactor
- venting port
- inlet mouth
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Abstract
The utility model relates to a photobioreactor device, and in particular relates to a photobioreactor device for culturing microalgae in high density. The photobioreactor device is characterized by comprising a photobioreactor, wherein a light source emitter is sleeved outside the photobioreactor; a photobioreactor outlet and a gas inlet are formed in the bottom of the photobioreactor; a feeding port and a gas exhaust are formed in the upper part of the photobioreactor; a detection device, a gas mixing device and a gas storage tank for an inlet CO2 gas are connected with the gas inlet in sequence; the feeding port is connected with a peristaltic pump; a gas exhaust CO2 gas detection device is connected with the gas exhaust; a gas exhaust gas drier is positioned between the gas exhaust CO2 gas detection device and the gas exhaust. The photobioreactor device is unique in structure, convenient to repair, capable or sufficiently utilizing optical energy and fixing the CO2 gas and solving the problems that the optical energy supply is insufficient, the CO2 gas utilization rate is low and the like when a sealed type photobioreactor is used for culturing microalgae in high density.
Description
Technical field
The utility model relates to a kind of optical-biological reaction apparatus, especially relates to the optical-biological reaction apparatus of the little algae of a kind of high-density culture.
Background technology
Day by day serious along with the day by day exhausted and environmental pollution of fossil energy makes human demand to the exploitation new resources also more and more urgent, and little algae is the very good material of preparation bioenergy, and the little algae of development and utilization will be the important channel of the human energy problem of solution.Simultaneously, little algae also has good application prospect at aspects such as foodstuffs industry, medicine industry, biotechnology and environmental purification.Along with the continuous intensification of the mankind to little algae understanding, the applied research of the new and effective bioreactor of development and the little algae of high-density culture thereof has become a study hotspot of Microalgae biotechnology.
Present little algae is cultivated and mainly contains open and closed two class bioreactors, and the Race-way photobioreactor simple structure is with low cost and simple to operate, but existence easily is subjected to the problems such as outside contamination, culture condition be unstable.Closed photo bioreactor can carry out aseptic technique, and culture condition is stable, but high-density culture has become Future Development trend.At present, utilize closed photo bioreactor to cultivate little algae, owing to having luminous energy undersupply, CO
2The problems such as utilization ratio is low have hindered the high-density growth of little algae to a certain extent.
Traditional closed photo bioreactor cost is relatively expensive and have the following disadvantages when cultivating little algae: 1) efficiency of light energy utilization is low.The daylighting way of closed photo bioreactor has outdoor direct daylighting and artificial light daylighting dual mode, outdoor daylighting exist photoelectric current low, be subject to the problems such as such environmental effects, be unfavorable for that little algae cultivates for a long time.Artificial light is divided into built-in light source and external light source, and built-in light source has increased the bioreactor cost, complex structure, not easy-maintaining; And external light source is generally by light source and directly carries out scattering, and optical energy loss is large, and utilization ratio is low; 2) CO
2Utilization ratio is not high.Little algae carries out the photosynthesis growth needs and absorbs CO
2, and CO
2Air Bubble Size then is the photosynthetic important factor of the little algae of impact.At present, generally use the refinement CO such as common aeration head and tunica fibrosa
2Carry out air feed, but this method CO
2Bubble diameter is large in algae liquid, the residence time is too short, solubleness is lower, and unfavorable little algae absorbs and transforms.Also there is not at present solution.
Summary of the invention
The purpose of this utility model is to overcome the deficiencies in the prior art, provide a kind of structure unique, easy to maintenance, can efficiently utilize luminous energy and fixation of C O
2Gas, the little algae of solution closed photo bioreactor high-density culture exist luminous energy undersupply, CO
2The optical-biological reaction apparatus of the little algae of a kind of high-density culture of the problems such as gas effciency is low.
The utility model is realized in the following way:
The optical-biological reaction apparatus of the little algae of a kind of high-density culture, it is characterized in that: it comprises that an overcoat has the bioreactor of light source emitter, the bioreactor bottom is provided with the reactor discharge port and inlet mouth, top are provided with opening for feed and venting port, and above-mentioned inlet mouth is connected with inlet mouth CO successively
2The proofing unit of gas, gas mixer and gas-holder, described opening for feed is connected with peristaltic pump, venting port CO
2Gas-detecting device is connected with venting port, and the venting port gas flow dryer is positioned at venting port CO
2Between gas-detecting device and the venting port;
Described gas mixer comprises a gas mixing steel cylinder, gas mixing steel cylinder one upper lateral part and described inlet mouth CO
2Be connected with reducing valve, micrometering valve and gas meter between the proofing unit of gas in turn, be connected with non-return valve between opposite side bottom and the described gas-holder;
In described bioreactor bottom centre CO is set
2Gas atomizer, an end of refining device is connected with described inlet mouth, is provided with the whipping appts with twayblade at described bioreactor cylindrical shell center, prevents algae liquid the swirl baffle plate of phenomenon being fixed with of described bioreactor inwall all around;
Described light source emitter comprises iron meshwork lamp frame cylindraceous and many energy-saving daylight lamps that are installed on this lamp bracket, and this fluorescent lamp top is connected with the light intensity regulating switch, the outer included light-transmissive film of attached tapetum lucidum of described lamp bracket;
The Glass Containers of the employing plug sealing bottleneck that described bioreactor main body is normal transparent.
The utility model has following effect:
1) structure is unique, easy to maintenance: it comprises that an overcoat has the bioreactor of light source emitter the device that the utility model provides, the bioreactor bottom is provided with the reactor discharge port and inlet mouth, top are provided with opening for feed and venting port, and above-mentioned inlet mouth is connected with inlet mouth CO successively
2The proofing unit of gas, gas mixer and gas-holder, described opening for feed is connected with peristaltic pump, venting port CO
2Gas-detecting device is connected with venting port, and the venting port gas flow dryer is positioned at venting port CO
2Between gas-detecting device and the venting port; This device comprises light source emitter, bioreactor, light source emitter, is arranged on the whipping appts in the bioreactor, the online detection inlet mouth CO that is connected with bioreactor respectively
2The proofing unit of gas and venting port CO
2The proofing unit of gas; Described light source emitter is circular ring structure, and outer attached tapetum lucidum can be regulated optical path distance and light intensity on request, and illumination is even, and the light Multi reflection improves the efficiency of light energy utilization, reduces the luminous energy material requirement of reactor; Bottom centre arranges CO at bioreactor
2Gas atomizer, this refining device is connected with inlet mouth, presses the real gas composition requirement, with CO
2The online detected value of gas-detecting device is as the criterion, and accurate adjust flux meter is with CO
2With pressurized air input gas mixer, mixed gas is dispersed into diameter less than the small bubbles of 0.1mm through refining device, and under the effect of agitating vane, laterally sufficient photosynthesis is carried out in circulation; Described light source emitter comprises circular iron meshwork lamp frame and is installed in many energy-saving daylight lamps on this lamp bracket, and this fluorescent lamp top is connected with the light intensity regulating switch.Light source emitter is external light source, be not connected with bioreactor, independently be coated at around the bioreactor, many energy-saving daylight lamps are installed on the circular iron meshwork lamp frame equidistantly, this circle iron meshwork lamp frame has controllability, can regulate in the certain diameter scope, to satisfy different microalgae growth phase bioreactor surface incident intensity, when regularly also can carrying out light intensity by the light intensity by-pass cock, regulates diameter one, this structure has easy to maintenance, the characteristics such as simple in structure, not only reduce the complicacy of bioreactor structure, increased its flexibility ratio, and had larger illumination surface-area and volume ratio.
2) improve the efficiency of light energy utilization: the outer attached tapetum lucidum of the circular iron meshwork lamp of the device that the utility model provides frame, in the middle of included light-transmissive film, fluorescent tube placed, whether the installation of light-transmissive film can adjust accordingly according to the algae liquid temp.The outer attached tapetum lucidum of circular iron meshwork lamp frame, make the light Multi reflection, increase little algae photosynthesis number of times, improve the efficiency of light energy utilization, included light-transmissive film significantly stops optical radiation to be delivered to algae liquid under the condition that does not affect light transmission, effect with regulating and controlling temperature, but at Initial stage of culture, frond is few to the light demand, detachably to keep the optical-biological reaction actuator temperature.
3) safe and reliable: the described bioreactor main body of the device that the utility model provides is the conventional clear glass container, be simple and easy to, use plug sealing bottleneck, reinforced by chuck between plug and the bottleneck, prevent gas leakage, plug is provided with reactor feed mouth and venting port, is provided with reactor discharge port and inlet mouth in its bottom, described opening for feed is connected with peristaltic pump, described venting port CO
2Gas-detecting device is connected with venting port; Be provided with the whipping appts with twayblade at the reactor shell center, and baffle plate is set prevents the algae liquid phenomenon that swirls.System is provided with the twayblade whipping appts, and gas-liquid two-phase is fully mixed, and also has heat sinking function, keeps constant temperature; Described gas mixer comprises gas mixing steel cylinder, reducing valve, micrometering valve, gas meter, the non-return valve that connects successively.Inlet mouth CO
2Gas-detecting device is connected between gas meter and the non-return valve in the mode of series connection.High pressure gas in the gas mixing steel cylinder suitably reduce pressure through reducing valve, play protection CO
2The effect of gas-detecting device, but air pressure still is higher than algae liquid hydraulic pressure, safeguards system trouble-free operation after the decompression.
4) efficient fixation of C O
2With the little algae of high-density culture: the device that the utility model provides arranges CO in bioreactor bottom centre
2Gas atomizer, this refining device is with CO
2Gas dispersion becomes diameter fully to mix with algae liquid less than the small bubbles of 0.1mm, and an end of refining device is connected with inlet mouth.Because CO
2Bubble is small, has prolonged CO
2The residence time in algae liquid, and under the stirring of twayblade CO
2Gas laterally circulates with algae liquid, increases CO
2The contact area of gas and algae liquid, and further improve CO
2The residence time of gas in algae liquid, promote the photosynthesis of little algae, to reach efficient fixation of C O
2Purpose with the little algae of high-density culture.
Description of drawings
Fig. 1 is structural representation of the present utility model;
Fig. 2 is the structural representation of the utility model light source emitter.
Embodiment
The optical-biological reaction apparatus of the little algae of a kind of high-density culture, as shown in Figure 1: it comprises that an overcoat has the bioreactor 11 of light source emitter 10, bioreactor 11 bottoms are provided with reactor discharge port 8 and inlet mouth 24, top are provided with opening for feed 21 and venting port 22, and above-mentioned inlet mouth 24 is connected with inlet mouth CO successively
2The proofing unit 6 of gas, gas mixer and gas-holder 1, described opening for feed 21 is connected with peristaltic pump 12, venting port CO
2Gas-detecting device 7 is connected with venting port 22, and venting port gas flow dryer 23 is positioned at venting port CO
2Between gas-detecting device 7 and the venting port 22.In bioreactor 11, also be provided with automatic detection device 13.
Described gas mixer comprises a gas mixing steel cylinder 2, gas mixing steel cylinder 2 one upper lateral parts and described inlet mouth CO
2Be connected with reducing valve 3, micrometering valve 4 and gas meter 5 between the proofing unit 6 of gas in turn, be connected with non-return valve 15 between opposite side bottom and the described gas-holder 1.
In described bioreactor 11 bottom centre CO is set
2Gas atomizer 7, one end of refining device 7 is connected with described inlet mouth 24, be provided with the whipping appts 14 with twayblade at described bioreactor 11 cylindrical shell centers, prevent algae liquid the swirl baffle plate 9 of phenomenon being fixed with of described bioreactor 11 inwalls all around.
Described light source emitter 10 comprises iron meshwork lamp frame 16 cylindraceous and the many energy-saving daylight lamps 18 that are installed on this lamp bracket, and these fluorescent lamp 18 tops are connected with the light intensity regulating switch, described lamp bracket 16 outer attached tapetum lucidum 17 included light-transmissive films 20.
The Glass Containers of the employing plug sealing bottleneck that described bioreactor 11 main bodys are normal transparent.
The device that the utility model provides is mainly used in the cultivation of little algae, and concrete operations are as follows:
1) before little algae is cultivated, after all parts of described device are installed, close all valves, open opening for feed 21 valves, water flushing bioreactor 2-3 time is opened and is closed after discharge port 8 valves drain water.Saturated high-temperature water vapor is passed into inside reactor by opening for feed 21 carry out high-temp steam sterilizing.With the aseptic culture medium for preparing in advance, pump into reactor by reactor feed mouth 21, access is in the algae kind of logarithmic phase and closes opening for feed 21 valves.In the culturing process of little algae, according to the optical attenuation equation of little algae, the incident intensity under the control different microalgae concentration.Lower at the Initial stage of culture algae cell density, the optical attenuation degree is little, can by on-off control and quantity and the light intensity of regulating fluorescent lamp 18, light-transmissive film 20 be installed; Along with the increase of algae cell density, frustule is to the absorption of luminous energy and block all and strengthen, and corresponding adjusting fluorescent lamp quantity and light intensity are installed light-transmissive film 20 absorb light radiation.
2) after the access of algae kind, regulate mixed gas composition ratio and flow velocity by gas meter 5 and micrometering valve 4, open inlet mouth 24, mixed gas is through CO
2Refining device 7 is dispersed into diameter and enters bioreactor 11 less than the small bubbles of 0.1mm, the stirring bubble of whipping appts 14 laterally circulation fully mix with algae liquid.According to the little concentration of algae of different times, suitably regulate CO
2Air input and concentration.
3) cultivate after 6-7 days, open discharge port 8 algae liquid collection tube valves, utilize ultraviolet spectrophotometer to measure after the sampling, when concentration is larger, gather with collector.
Above-mentioned detailed description is that this embodiment limits claim of the present invention for the specifying of possible embodiments of the present invention, and the equivalence that all the present invention of disengaging do is implemented or change, all should be contained in the claim of this case.
Claims (5)
1. the optical-biological reaction apparatus of the little algae of high-density culture, it is characterized in that: it comprises that an overcoat has the bioreactor (11) of light source emitter (10), bioreactor (11) bottom is provided with reactor discharge port (8) and inlet mouth (24), top are provided with opening for feed (21) and venting port (22), and above-mentioned inlet mouth (24) is connected with inlet mouth CO successively
2The proofing unit of gas (6), gas mixer and gas-holder (1), described opening for feed (21) is connected venting port CO with peristaltic pump (12)
2Gas-detecting device (7) is connected with venting port (22), and venting port gas flow dryer (23) is positioned at venting port CO
2Between gas-detecting device (7) and the venting port (22).
2. the optical-biological reaction apparatus of the little algae of a kind of high-density culture as claimed in claim 1, it is characterized in that: described gas mixer comprises a gas mixing steel cylinder (2), gas mixing steel cylinder (2) one upper lateral parts and described inlet mouth CO
2Be connected with reducing valve (3), micrometering valve (4) and gas meter (5) between the proofing unit of gas (6) in turn, be connected with non-return valve (15) between opposite side bottom and the described gas-holder (1).
3. the optical-biological reaction apparatus of the little algae of a kind of high-density culture as claimed in claim 1, it is characterized in that: bottom centre arranges CO at described bioreactor (11)
2Gas atomizer (7), one end of refining device (7) is connected with described inlet mouth (24), be provided with the whipping appts (14) with twayblade at described bioreactor (11) cylindrical shell center, prevent algae liquid the swirl baffle plate (9) of phenomenon being fixed with of described bioreactor (11) inwall all around.
4. the optical-biological reaction apparatus of the little algae of a kind of high-density culture as claimed in claim 1, it is characterized in that: described light source emitter (10) comprises iron meshwork lamp frame cylindraceous (16) and is installed in many energy-saving daylight lamps (18) on this lamp bracket, this fluorescent lamp (18) top is connected with the light intensity regulating switch, the outer included light-transmissive film of attached tapetum lucidum (17) (20) of described lamp bracket (16).
5. the optical-biological reaction apparatus of the little algae of a kind of high-density culture as claimed in claim 1 is characterized in that: described bioreactor (11) main body is the Glass Containers of the employing plug sealing bottleneck of normal transparent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320254359 CN203238266U (en) | 2013-05-13 | 2013-05-13 | Photobioreactor device for culturing microalgae in high density |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320254359 CN203238266U (en) | 2013-05-13 | 2013-05-13 | Photobioreactor device for culturing microalgae in high density |
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| CN203238266U true CN203238266U (en) | 2013-10-16 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN 201320254359 Expired - Fee Related CN203238266U (en) | 2013-05-13 | 2013-05-13 | Photobioreactor device for culturing microalgae in high density |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103224879A (en) * | 2013-05-13 | 2013-07-31 | 宁夏大学 | Photobioreactor device for high-density culturing of microalgae |
| CN104109631A (en) * | 2014-07-16 | 2014-10-22 | 中国科学院青岛生物能源与过程研究所 | Microalgae culture reactor |
| CN109880741A (en) * | 2019-04-04 | 2019-06-14 | 重庆大学 | A kind of stabilization culture apparatus mixing microalgae |
-
2013
- 2013-05-13 CN CN 201320254359 patent/CN203238266U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103224879A (en) * | 2013-05-13 | 2013-07-31 | 宁夏大学 | Photobioreactor device for high-density culturing of microalgae |
| CN104109631A (en) * | 2014-07-16 | 2014-10-22 | 中国科学院青岛生物能源与过程研究所 | Microalgae culture reactor |
| CN109880741A (en) * | 2019-04-04 | 2019-06-14 | 重庆大学 | A kind of stabilization culture apparatus mixing microalgae |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20131016 Termination date: 20160513 |