CN101935610B - Multi-group bubbling type photobioreactor - Google Patents
Multi-group bubbling type photobioreactor Download PDFInfo
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- CN101935610B CN101935610B CN201010244909.3A CN201010244909A CN101935610B CN 101935610 B CN101935610 B CN 101935610B CN 201010244909 A CN201010244909 A CN 201010244909A CN 101935610 B CN101935610 B CN 101935610B
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- 238000010586 diagram Methods 0.000 description 6
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- 241000195649 Chlorella <Chlorellales> Species 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 2
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
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Abstract
The invention discloses a multi-group bubbling type photobioreactor which comprises a box body, bubbling type photobiologic reaction units, a main air duct, light sources and a control system, wherein at least two bubbling type photobiologic reaction units are placed in rows in the box body, the main air duct is connected to an air-blowing pipe and a gas distributor in each bubbling type photobiologic reaction unit through gas-distributing branch pipes, the light sources are placed at both sides of each bubbling type photobiologic reaction unit, and the control system comprises a temperature control system and a light source control system for controlling the temperature in the box body and the intensity of the light sources. The invention supplies a batched, controllable and mature facility for the microalgae culture in laboratory and bench scales.
Description
Technical field
The invention belongs to the biological reaction apparatus technical field, relate to a kind of many group bubbling style bioreactors, especially design a kind of many groups bubbling style bioreactor that little algae is cultivated that is applicable to.
Background technology
Little algae is as a kind of important biomass resource, have distribute wide, biomass is large, CO
2The outstanding features such as utilization ratio is high, environmental compatibility is strong, growth cycle is short and output is high are the excellent materials that carries out biorefinery, and it has wide DEVELOPMENT PROSPECT producing little algae fuel, the little algae biotechnological formulation of exploitation and extract the aspects such as biologically active substance.The various bioactivators such as many little algae rich in proteins, polysaccharide, grease, carotenoid and unsaturated fatty acids are human important Biological resources storehouses.Algae resource has become food, medicine, the important treasure-house of bioenergy product.Little algal biomass is the good raw material of producing biologically active substance, biomaterial and biofuel.Utilize little algal biomass resource such as little algae or engineering microalgae to produce fossil substitution of resources product, biofuel particularly, can be Biological Energy Industry and open up a new Technology Ways, can effectively solve the raw material bottleneck of biofuel industry, for the health of biofuel industry, stable and Sustainable development provide new thinking, thereby realize substituting and socioeconomic Sustainable development of fossil resource.At present.The study hotspot in the whole world all begins to concentrate on little this technology of algae biorefinery.Little algae biorefinery technology namely refers to cultivate by the bioreactor mass-producing of microalgae cell take photosynthesis as the basis, produces a kind of technology of biomass fuel and bio-active products
The global research and development tide of little algae arrives, but the R﹠D work of countries in the world in this field also rests on the starting stage of experimental study and pilot scale demonstration basically, not yet realizes the mass-producing preparation.This mainly is because the technological difficulties that exist are more in little algae R﹠D process, and wherein the development as the high-efficiency photobioreactor of Study of Support is one of Main Bottleneck, so this direction is that little algae is cultivated and little algae fixation of C O always
2The study hotspot of technology.
The target of bioreactor optimization design relates generally to four aspects: the specific surface area of (1) augmenting response device.The form of bioreactor is various, has openly, and closed (main tube-separating type, board-like and bevel-type three classes) is also arranged, and wherein open specific surface area is larger; (2) strengthen gas-liquid mass transfer efficient.Generally improve gas-liquid mass transfer effect in little algae culturing process by designing attached device architecture, such as gas liquid exchanger, condenser, ultra-filtration equipment, mixing apparatus and nutraceutical feeding mechanism; (3) provide high efficiency light source.Studies show that in a large number little algae absorbs CO
2Produce O
2Speed largely depends on illumination condition.Improving illumination condition need to solve two problems, and one is that high efficiency light source provides, and another is the effective distribution of light in reactor.Except utilizing sunlight and common luminescent lamp as the light source, also developed and utilized light emitting diode, photoconductive fiber and photoflash lamp as the various bioreactors of light source.The position of light source can be divided into external placed type and built-in two kinds, and the built-in distribution that can significantly improve light improve the light source utilization ratio, but cost of manufacture is higher.In addition, various illumination operational conditions such as light quality, light intensity, Light To Dark Ratio and light application time etc., also have a significant impact micro algae growth; (4) improve the light transfer efficiency.Research finds that the simple light intensity that improves can not correspondingly improve the product O of little algae
2Speed.Take Chlorella kessleri as the algae kind, utilize photodiode as the bioreactor of light source, when frond density reaches 5 * 10
8During cells/mL, O
2Clean generation speed is 10mmol O
2/ (Lh).When frond was increased to certain density, its apparent product oxygen speed (OPR) began to descend, and if masking phenomenon each other not between the frond in the bioreactor, O
2Throughput rate will improve at least 5 times.
Have more technological difficulties in little algae R﹠D process, wherein lack the bioreactor of efficient, energy-conservation, technology maturation as Research foundation, it has limited little algae research and development and large-scale development greatly.This mainly is because the microalgae photobiological reactor design relates to more multi-disciplinary intersection, and background science principle and the rule of its design systematically do not understood and grasped in present research from profound level, therefore is difficult to form the core technology that solves key issue.Develop low cost, efficient, energy-conservation microalgae photobiological reactor design technology, will lay a solid foundation for the research of Microalgae biotechnology undoubtedly, hold the bioeconomic initiative of following little algae.
Summary of the invention
Technical problem to be solved by this invention provides many groups bubbling style bioreactor that a kind of layout is succinct, practical, be used for small-scale production, to fill the domestic gaps.
For solving the problems of the technologies described above, the technical solution used in the present invention is as follows:
A kind of many group bubbling style bioreactors, it comprises casing, bubbling style optical-biological reaction unit, main gas tube, light source and Controlling System, plural bubbling style optical-biological reaction unit in a row places in the casing, main gas tube is connected by the gas distributor of the aspiration tube in gas distribution arm and the bubbling style optical-biological reaction unit and aspiration tube bottom, light source places the both sides of every row's bubbling style optical-biological reaction unit, and Controlling System comprises that temperature controlling system and light-source control system are in order to control temperature in the casing and the intensity of light source.
Wherein, casing is provided with openable lid.
Wherein, cabinet wall scribbles light reflecting material.
Wherein, be equipped with a main gas tube on each top, row's bubbling style optical-biological reaction unit.
Wherein, each root gas distribution arm is provided with gas meter.
Wherein, described bubbling style optical-biological reaction unit is made by high light transmissive material, and the lower end circular arc is received the end, closes up and polish in the upper end, with the soft rubber ball sealing, reserves inlet mouth, air outlet, material-feeding port and electrode socket on the soft rubber ball.
Wherein, the wavelength of light source disposes as requested, and wave band is any wave band among the 250nm-780nm.
Wherein, light source is tubular lamp, from approaching along the position near many tubular lamps of the even parallel distribution of bottom position of bubbling style optical-biological reaction unit.
Wherein, the light source between adjacent two row's bubbling style optical-biological reaction unit is single or double.
Wherein, be provided with a baffle plate between adjacent two row's bubbling style optical-biological reaction unit, baffle plate is divided into multicell with casing, and baffle surface scribbles light reflecting material.
Beneficial effect: the relative prior art of the present invention has following advantage and effect:
1, adopts the body structure of particular design.This device box inwall scribbles light reflecting material, can prevent effectively that light from leaking, and increases intensity of illumination, improves the efficiency of light energy utilization.Folding design sealing rationally can effectively be guaranteed effective control of temperature and luminous energy.
2, adopt microcomputer-recognized intelligent automatic light source Controlling System.This device the Lights section automatic control system passes through to regulate bright light quantity controlled light intensity, and can realize the multistage light source control by control, secretly circulates such as light, and the cyclic switching of different light intensity also can be replaced according to the requirement of light source wave band.
3, light source, bio-reactor team design.The independent cabin of this device reaction device part.Can realize independent component air feed and the intensity control in cabin.In addition, can realize the Ventilation Rate control of independent light bio-reactor.Simple for structure, easy to operate, temperature controllable, light intensity, light quality, Ventilation Rate, gaseous fraction, but batch processing.
4, the intensity of light source and light quality are controlled.This illumination cultivation system source adopts the replaceable design, can change light source according to the growing state of different algae kinds and little algae.This device the Lights section can be according to the biomass required light quality of growing, the light source of configuration Different lightwave section, and optical band can be from infrared to any wave band of near ultraviolet.
5, temperature intelligent is controlled.This illumination cultivation system contains the multi-point temp sensing, and refrigeration compressor, electro-thermal heating system have realized that the light temperature in the illumination cultivation system is accurately controlled.
Description of drawings
Fig. 1 is the main TV structure synoptic diagram of many group bubbling style bioreactors of the present invention.
Fig. 2 is the plan structure synoptic diagram of many group bubbling style bioreactors of the present invention.
Fig. 3 is the left TV structure synoptic diagram of many group bubbling style bioreactors of the present invention.
Fig. 4 is many rows light source mode of many group bubbling style bioreactors of the present invention.
Fig. 5 is a kind of mode that many group bubbling style bioreactors of the present invention use baffle plate.
Fig. 6 is the another kind of mode that many group bubbling style bioreactors of the present invention use baffle plate.
Embodiment
According to following embodiment, the present invention may be better understood.Yet, those skilled in the art will readily understand that embodiment is described only to be used for explanation the present invention, and should also can not limit the present invention described in detail in claims.
Embodiment 1:
Fig. 1 is the main TV structure synoptic diagram of many group bubbling style bioreactors of the present invention, and Fig. 2 is its plan structure synoptic diagram, and Fig. 3 is its left TV structure synoptic diagram.Can clearly understanding be arranged to apparatus structure of the present invention by Fig. 1～3.Many group bubbling style bioreactors of the present invention mainly comprise casing 1, bubbling style optical-biological reaction unit 2, main gas tube 3, light source 4 and Controlling System 5.
Several bubbling style optical-biological reaction unit 2 in a row place in the casing 1, be equipped with a main gas tube 3 on each 2 top, row's bubbling style optical-biological reaction unit, the gas distributor 7 of main gas tube 3 by the aspiration tube 6 in each root gas distribution arm 12 and each bubbling style optical-biological reaction unit and aspiration tube bottom is connected and carries out bubbling, gas distributor 7 adopts the alumina high temperature calcining to form or macromolecular material is made, mixed gas is distributed in the nutrient solution with atomic little bubble, in order to control separately the gas flow of each optical-biological reaction unit, on each root gas distribution arm 6, and be positioned at 2 tops, bubbling style optical-biological reaction unit and be provided with gas meter 9.The setting of gas meter is to establish for independent control gas flow; also can directly use valve control; valve or gas meter are set to control simultaneously the gas flow rate of optical-biological reaction in a row unit 2 in main gas tube 3; perhaps in the portion gas distribution arms valve or gas meter are set and do not affect essence of the present invention with the gas flow rate of control section optical-biological reaction unit 2; all within protection scope of the present invention; any valve or gas meter even are not set; optical-biological reaction unit 2 in the whole casing all adopts identical gas flow rate, also within protection scope of the present invention.Main gas tube 3 external airing systems, airing system is comprised of source of the gas, gas mixer, buffer container, strainer, gas meter, and those skilled in the art can realize the assembling of airing system according to prior art.
Above-mentioned bubbling style optical-biological reaction unit 2 is made by high light transmissive material, such as high-boron-silicon glass.Its lower end circular arc is received the end, closes up and polish in the upper end, with soft rubber ball 10 sealings, reserves inlet mouth, air outlet, material-feeding port and electrode socket (such as temperature electrode or pH electrode) on the soft rubber ball.The present invention to the quantity of bubbling style optical-biological reaction unit 2, size, arrangement mode without any restriction; those skilled in the art can be according to cultivation demand and the needed industrial scale of different microalgae; the adaptive change that bubbling style optical-biological reaction unit 2 is carried out in shape; select the optical-biological reaction unit of suitable dimension specification; determine suitable element number; the setting of arranging, and the size of determining adaptive casing, above-mentioned variation is all within protection scope of the present invention.
Casing 1 is provided with openable lid 8, makes things convenient for the taking-up of bubbling style optical-biological reaction unit 2 to insert, and also makes things convenient for the replacing of light source 4, and the present invention does not do any restriction to position and the size of lid, can be arranged on the top of sidewall or the casing of casing.For the ease of observing the response situation of casing 1 inside, also can a viewing window be set at casing 1.Casing 1 inwall scribbles light reflecting material, realizes that luminous energy effectively utilizes.Casing can adopt double-layer sealing structure, to keep temperature in the casing.Casing 1 leaves inlet pipe road junction and escape pipe road junction usually.
Controlling System 5 comprises that temperature controlling system and light-source control system are in order to control temperature in the casing and the intensity of light source.Temperature controlling system comprises: refrigeration compressor, blower fan electric fan, electric heating system, multipoint temperature sensor, micro computer temperature control system.Those skilled in the art can be controlled according to the temperature that prior art manifests in the casing.Light-source control system 5 for example can be realized the controlled of intensity of illumination by the bright light quantity of control fluorescent tube by microcomputer-recognized intelligent control lighting system, and all right setup times circulation allows light source produce illumination within certain period, extinguishes within certain period.Those skilled in the art can work out light level according to prior art.
As an optimal way of the present invention, can between adjacent two row's bubbling style optical-biological reaction unit 2, baffle plate 11 be set, baffle plate 11 surfaces scribble light reflecting material, and baffle plate 11 is divided into multicell with casing 1, realizes that the intensity of light source of each chamber is independent.The present invention is the quantity of defining baffle not, can divide as required how many individual independently chambers, the baffle plate of respective numbers is set, for example can as shown in Figure 5, between all adjacent two row's bubbling style optical-biological reaction unit 2, baffle plate be set, so that each row's bubbling style optical-biological reaction unit 2 all forms independently space, realize the independence of intensity of illumination, also can between some adjacent two row's bubbling style optical-biological reaction unit 2, baffle plate be set as shown in Figure 6, casing is divided into two independently chambers.Whether baffle plate is set is not conflicted with the row of light source, for example can adopt the mode that two row's light sources are set between adjacent two row's bubbling style optical-biological reaction unit 2, baffle plate is inserted between two row's light sources (Fig. 5), also can adopt the mode that single light source is set between adjacent two row's bubbling style optical-biological reaction unit 2, baffle plate is inserted between single light source and the optical-biological reaction unit, has light source (Fig. 6) as long as guarantee each chamber of dividing.The present invention does not do any restriction to the fixed form of baffle plate; can adopt casing draw-in groove fixed dam; perhaps support fixed dam; perhaps baffle plate being fixed on the first-class mode of light source can; even do not adopt any fixed form; directly baffle plate is inserted between light source and the light source or between light source and the optical-biological reaction unit, also in protection scope of the present invention and so on.
Can realize the control of temperature in the culturing process, light intensity, light quality, Ventilation Rate, gaseous fraction by many group bubbling style bioreactors of the present invention.
Embodiment 2:
Cultivate chlorella at many groups bubbling style bioreactor that embodiment 1 describes, with bubbling style optical-biological reaction unit (diameter 10cm, high be 60cm) in the f/2 substratum of packing into, casing is sterilized outward, the access chlorella, inoculum size is 0.2g/L, reinstall in the casing, main gas tube, gas distribution arm are connected with gas distributor with aspiration tube, plug temperature electrode or pH electrode, plug the air outlet, the jam-pack soft rubber ball.Open air distributing device, by the ratio of gas meter control carbonic acid gas and air, open the valve on the gas distribution arm, bubbling in the bubbling style optical-biological reaction unit.As shown in Figure 5, the controlled light intensity in four chambeies of reactor is followed successively by 3000lux, 5000lux, 70000lux, and 9000lux, spin manifold temperature are controlled at 26 ℃, CO
2Ventilation content be respectively 1% (v/v), 3% (v/v), 5% (v/v), 7% (v/v), cultivated for two weeks after, biomass is up to 2.253g/L, fat content is 35%.Experiment is in time cleaned bubbling style optical-biological reaction unit after finishing, and waits until next time and uses.
Claims (8)
1. organize the bubbling style bioreactor one kind more, it is characterized in that it comprises casing (1), bubbling style optical-biological reaction unit (2), main gas tube (3), light source (4) and Controlling System (5), plural bubbling style optical-biological reaction unit (2) in a row places in the casing (1), main gas tube (3) is connected by the aspiration tube (6) in gas distribution arm (12) and the bubbling style optical-biological reaction unit and the gas distributor (7) of aspiration tube bottom, light source (4) places the both sides of every row's bubbling style optical-biological reaction unit (2), and Controlling System (5) comprises that temperature controlling system and light-source control system are in order to control temperature in the casing and the intensity of light source;
Described bubbling style optical-biological reaction unit (2) is made by high light transmissive material, and the lower end circular arc is received the end, closes up and polish in the upper end, with soft rubber ball (10) sealing, reserves inlet mouth, air outlet, material-feeding port and electrode socket on the soft rubber ball;
Casing (1) is provided with openable lid (8).
2. many group bubbling style bioreactors according to claim 1 is characterized in that casing (1) inwall scribbles light reflecting material.
3. many group bubbling style bioreactors according to claim 1 is characterized in that being equipped with a main gas tube (3) on each top, row bubbling style optical-biological reaction unit (2).
4. many group bubbling style bioreactors according to claim 1 is characterized in that on each root gas distribution arm (6) gas meter (9) being housed.
5. many group bubbling style bioreactors according to claim 1, the wavelength that it is characterized in that light source (4) is 250nm～780nm.
6. many group bubbling style bioreactors according to claim 1 is characterized in that light source (4) be tubular lamp, go up along the position near many tubular lamps of the even parallel distribution of bottom position from approaching of bubbling style optical-biological reaction unit (2).
7. according to claim 1 or 6 described many group bubbling style bioreactors, it is characterized in that light sources (4) between the adjacent two row bubbling style optical-biological reaction unit (2) are for single or double.
8. many group bubbling style bioreactors according to claim 1, it is characterized in that being provided with a baffle plate (11) between the adjacent two row bubbling style optical-biological reaction unit (2), baffle plate (11) is divided into multicell with casing (1), and baffle plate (11) surface scribbles light reflecting material.
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| CN201010244909.3A CN101935610B (en) | 2010-08-04 | 2010-08-04 | Multi-group bubbling type photobioreactor |
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| CN201010244909.3A CN101935610B (en) | 2010-08-04 | 2010-08-04 | Multi-group bubbling type photobioreactor |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IT201900002641A1 (en) * | 2019-02-25 | 2020-08-25 | Milano Politecnico | Modular kit for integration and installation of one or more bioreactors for the cultivation of microalgae |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2014130357A1 (en) * | 2013-02-19 | 2014-08-28 | Heliae Development, Llc | Bioreactor array and methods of combinatorial testing |
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| WO2020174351A1 (en) * | 2019-02-25 | 2020-09-03 | Politecnico Di Milano | Modular kit for integration and installation of one or more bioreactors for microalgae cultivation |
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| CN101935610A (en) | 2011-01-05 |
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