CN103642691B - Deep-hole cell culture plate - Google Patents
Deep-hole cell culture plate Download PDFInfo
- Publication number
- CN103642691B CN103642691B CN201310668179.3A CN201310668179A CN103642691B CN 103642691 B CN103642691 B CN 103642691B CN 201310668179 A CN201310668179 A CN 201310668179A CN 103642691 B CN103642691 B CN 103642691B
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- CN
- China
- Prior art keywords
- culture plate
- cultivation
- deep
- hole
- deep hole
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/12—Well or multiwell plates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/24—Gas permeable parts
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
Abstract
Patent of the present invention provides Deep-hole cell culture plate, can provide sufficient oxygen for the cultivation of aerobic cell, and then improves the dissolved oxygen coefficient of culture plate.This culture plate is evenly distributed with cultivation deep hole, it is characterized in that: the inner homogeneous of the base plate of culture plate is furnished with gas passage, gas passage is provided with the inlet mouth with each air outlet of cultivation deep hole UNICOM and the external communication with culture plate, be positioned at end, each air outlet, the base plate of culture plate is convex is provided with hollow air guide post.
Description
Technical field
Patent of the present invention relates to cell culture apparatus technical field, particularly relates to the Deep-hole cell culture plate of the high-throughput for aerobic cell, high-density culture.
Background technology
Tissue Culture Plate can utilize less nutrient solution to carry out the cultivation of cell high-throughput and screen with bacterial classification or cell strain, have fast, high-throughput, the feature such as cost-saving, in the process of cell cultures, the general Tissue Culture Plate that first uses carries out the cell cultures that flux is high, volume is little, filter out optimum bacterial classification, and tentatively determine preferably technique culture condition (temperature, pH value, substratum etc.), then filtered out bacterial classification and the processing condition tentatively determined are applied in fermentor tank, carry out scale amplification culture.
Oxygen is that aerobic Growth of Cells institute is required, is also one of important factor in order in scale amplification process simultaneously.Fermentor tank is mostly equipped with whipping appts or apparatus of oxygen supply, in aerobic cell cultivation process, fermentor tank can provide higher volume dissolved oxygen coefficient, and existing Deep-hole cell culture plate just simply by external the effect of certain rotating speed and eccentricity is provided under the nutrient solution in cultivation deep hole is vibrated, increase the contact area of nutrient solution and air, improve liquid mixing and carry out oxygen supply, in culturing process, gas-liquid is practised physiognomy little, liquid mixing effect is poor, the volume dissolved oxygen coefficient that culture plate is provided significantly is less than fermentor tank, due to the remarkable difference of volume dissolved oxygen coefficient, scale based on culture plate is amplified can not cultivation results in Accurate Prediction fermentor tank, the bacterial classification simultaneously using culture plate to filter out might not be best suited for fermentor tank.
Summary of the invention
For the problems referred to above, patent of the present invention provides Deep-hole cell culture plate, can provide sufficient oxygen for the cultivation of aerobic cell, and then improves the dissolved oxygen coefficient of culture plate.
Its technical scheme is such, Deep-hole cell culture plate, described culture plate is evenly distributed with cultivation deep hole, it is characterized in that: the inner homogeneous of the base plate of described culture plate is furnished with gas passage, described gas passage is provided with the inlet mouth with each described air outlet of cultivation deep hole UNICOM and the external communication with described culture plate, and the base plate of described culture plate is positioned at that the position of end, each air outlet is convex is provided with hollow air guide post.
It is further characterized in that:
Described gas passage comprises some branch roads be arranged in parallel, line number or the columns of the quantity of described branch road and the cultivation deep hole of described culture plate are equal, described branch road is provided with the air outlet with described cultivation deep hole UNICOM, the external communication of described branch road and described culture plate;
Described gas passage also comprises main line, and described main line is arranged at described base plate side, and is provided with the inlet mouth with the external communication of culture plate, described branch road one end and described main line UNICOM;
The height of described air guide post is more than or equal to the degree of depth of the described cultivation deep hole of 1/2nd, and preferably, the height of described air guide post is more than or equal to the degree of depth of the described cultivation deep hole of 2/3rds.
It is further characterised in that:
The inner circumferential of described cultivation deep hole is provided with four pieces of baffle plates, and described four pieces of baffle plates are uniform along side face;
Described culture plate is positioned at described inlet mouth place and is embedded with filtration unit, and described filtration unit comprises filter membrane;
Branch road and the described culture plate of described air guide post, described gas passage are integrated machine-shaping.
After adopting structure of the present invention, the inner homogeneous of the base plate of culture plate is furnished with gas passage, gas passage is provided with and the inlet mouth cultivating the air outlet of deep hole UNICOM and the external communication with culture plate, the base plate of culture plate is positioned at that the position of end, each air outlet is convex is provided with hollow air guide post, by inlet mouth UNICOM oxygen storage tank, cultivation for aerobic cell provides sufficient oxygen, and increase the oxygen partial pressure of cultivating deep hole inside, promote that oxygen diffusion is in nutrient solution, and then improve the dissolved oxygen coefficient of culture plate; By the setting of main line and branch road, can reduce inlet mouth, the connection of culture plate and external oxygen transfer line is convenient; / 3rd of a deep hole degree of depth is cultivated because the height generally cultivating added nutrient solution in deep hole is less than or equal to, the height of air guide post is set to the degree of depth of the cultivation deep hole being more than or equal to 1/2nd, can avoid in wave and culture process, nutrient solution is splashed in the through hole of air guide post; Baffle plate the eddy current that can destroy and produce in the shake process of nutrient solution is set, strengthen nutrient solution vibration, improve the mixed effect of air and nutrient solution, further increase the volume dissolved oxygen coefficient of Deep-hole cell culture plate; Be embedded with filtration unit at inlet mouth, can guarantee that aseptic oxygen enters in culture plate.
Accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
Fig. 2 is that the A-A of Fig. 1 is to structural representation;
Fig. 3 is that the B-B of Fig. 1 is to structural representation.
Embodiment
As shown in Figure 1 to Figure 3, Deep-hole cell culture plate, culture plate 1 is evenly distributed with cultivates deep hole 2, the inner homogeneous of the base plate 12 of culture plate 1 is furnished with gas passage, gas passage is provided with the inlet mouth with each air outlet 11 of cultivation deep hole 2 UNICOM and the external communication with culture plate 1, inlet mouth is position shown in right side arrow in Fig. 1, the base plate 12 of culture plate 1 is positioned at that the position of end, each air outlet 11 is convex is provided with hollow air guide post 8, the height h of air guide post 8 is more than or equal to the depth H of the cultivation deep hole 2 of 1/2nd, if nutrient solution vibration is violent, the height h of optional air guide post 8 is more than or equal to the depth H of the cultivation deep hole 2 of 2/3rds, gas passage comprises branch road 3, 4, 5, 6 and main line 7, the quantity of branch road is equal with the line number of the cultivation deep hole of culture plate, branch road 3, 4, 5, 6 are provided with the air outlet 11 with each cultivation deep hole 2 UNICOM, main line is arranged at base plate 12 side, and be provided with the inlet mouth with the external communication of culture plate 1, branch road 3, 4, 5, 6 one end and main line 7 UNICOM, cultivating the inner circumferential of deep hole 2, to be provided with four pieces of baffle plates, 9, four pieces of baffle plates 9 uniform along side face, culture plate 1 is positioned at inlet mouth place and is embedded with filtration unit 10, and filtration unit 10 comprises filter membrane, and the branch road 3,4,5,6 of air guide post 8, gas passage and culture plate 1 are integrated machine-shaping.
Claims (4)
1. Deep-hole cell culture plate, described culture plate is evenly distributed with cultivation deep hole, it is characterized in that: the inner homogeneous of the base plate of described culture plate is furnished with gas passage, described gas passage is provided with the inlet mouth with each described air outlet of cultivation deep hole UNICOM and the external communication with described culture plate, and the base plate of described culture plate is positioned at that the position of end, each air outlet is convex is provided with hollow air guide post; Described gas passage comprises some branch roads be arranged in parallel, line number or the columns of the quantity of described branch road and the cultivation deep hole of described culture plate are equal, described branch road is provided with the air outlet with described cultivation deep hole UNICOM, the external communication of described branch road and described culture plate; Described gas passage also comprises main line, and described main line is arranged at described base plate side, and is provided with the inlet mouth with the external communication of culture plate, described branch road one end and described main line UNICOM; Branch road and the described culture plate of described air guide post, described gas passage are integrated machine-shaping; The height of described air guide post is more than or equal to the degree of depth of the described cultivation deep hole of 1/2nd.
2. Deep-hole cell culture plate according to claim 1, is characterized in that: the height of described air guide post is more than or equal to the degree of depth of the described cultivation deep hole of 2/3rds.
3. Deep-hole cell culture plate according to claim 2, is characterized in that: the inner circumferential of described cultivation deep hole is provided with four pieces of baffle plates, and described four pieces of baffle plates are uniform along side face.
4. Deep-hole cell culture plate according to claim 3, is characterized in that: described culture plate is positioned at described inlet mouth place and is embedded with filtration unit, and described filtration unit comprises filter membrane.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310668179.3A CN103642691B (en) | 2013-12-11 | 2013-12-11 | Deep-hole cell culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310668179.3A CN103642691B (en) | 2013-12-11 | 2013-12-11 | Deep-hole cell culture plate |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103642691A CN103642691A (en) | 2014-03-19 |
| CN103642691B true CN103642691B (en) | 2015-09-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310668179.3A Expired - Fee Related CN103642691B (en) | 2013-12-11 | 2013-12-11 | Deep-hole cell culture plate |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105154318A (en) * | 2015-10-08 | 2015-12-16 | 上海理工大学 | Combined liquid fermentation shallow slot component and fermentation device thereof |
| CN111349550B (en) * | 2018-12-24 | 2022-12-23 | 中粮生物科技股份有限公司 | Device and method for fermenting aerobic strains at high flux and application of device and method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002034875A1 (en) * | 2000-10-24 | 2002-05-02 | Alexander Robert Powell | Cell culture apparatus |
| US20060292654A1 (en) * | 2005-06-28 | 2006-12-28 | Inogen, S.A. | Microwell plate |
| CN101935610A (en) * | 2010-08-04 | 2011-01-05 | 南京工业大学 | Multi-group bubbling type photobioreactor |
| CN203065490U (en) * | 2012-12-28 | 2013-07-17 | 江南大学 | Structure of cover of deep hole cell culture plate |
| CN203212577U (en) * | 2012-12-28 | 2013-09-25 | 江南大学 | Deep-hole cell culture plate with baffle plates |
-
2013
- 2013-12-11 CN CN201310668179.3A patent/CN103642691B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002034875A1 (en) * | 2000-10-24 | 2002-05-02 | Alexander Robert Powell | Cell culture apparatus |
| US20060292654A1 (en) * | 2005-06-28 | 2006-12-28 | Inogen, S.A. | Microwell plate |
| CN101935610A (en) * | 2010-08-04 | 2011-01-05 | 南京工业大学 | Multi-group bubbling type photobioreactor |
| CN203065490U (en) * | 2012-12-28 | 2013-07-17 | 江南大学 | Structure of cover of deep hole cell culture plate |
| CN203212577U (en) * | 2012-12-28 | 2013-09-25 | 江南大学 | Deep-hole cell culture plate with baffle plates |
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| Publication number | Publication date |
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| CN103642691A (en) | 2014-03-19 |
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