CN201737931U - Multi-group bubbling type photobioreactor - Google Patents
Multi-group bubbling type photobioreactor Download PDFInfo
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- CN201737931U CN201737931U CN201020282646.0U CN201020282646U CN201737931U CN 201737931 U CN201737931 U CN 201737931U CN 201020282646 U CN201020282646 U CN 201020282646U CN 201737931 U CN201737931 U CN 201737931U
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- 230000005587 bubbling Effects 0.000 title claims description 70
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- 241000197194 Bulla Species 0.000 abstract 5
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- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 1
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- 239000013543 active substance Substances 0.000 description 1
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- PNEYBMLMFCGWSK-UHFFFAOYSA-N aluminium oxide Inorganic materials [O-2].[O-2].[O-2].[Al+3].[Al+3] PNEYBMLMFCGWSK-UHFFFAOYSA-N 0.000 description 1
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/58—Reaction vessels connected in series or in parallel
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
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- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/02—Means for providing, directing, scattering or concentrating light located outside the reactor
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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Abstract
The utility model discloses a multiunit tympanic bulla formula photobioreactor, it includes the box, tympanic bulla formula photobioreactor unit, lead trachea, light source and control system, the interior row of tympanic bulla formula photobioreactor unit more than two is arranged in the box, lead trachea and gas distributor in the trachea is connected to tympanic bulla formula photobioreactor unit through the gas distribution branch connection, the both sides of tympanic bulla formula photobioreactor unit are arranged in to the light source, control system includes temperature control system and light source control system and is used for the intensity of the temperature in the control box and light source. The utility model provides a batch, controllable and mature device for microalgae cultivation in laboratories and small scale experiments.
Description
Technical field
The utility model belongs to the biological reaction apparatus technical field, relates to a kind of many group bubbling style bioreactors, especially designs a kind of many groups bubbling style bioreactor that is applicable to that little algae is cultivated.
Background technology
Little algae is as a kind of important biomass resource, have distribute wide, biomass is big, CO
2The utilization ratio height, environmental compatibility is strong, growth cycle is short and outstanding feature such as output height, is the excellent material that carries out biorefinery, and it has the wide development prospect producing little algae fuel, the little algae biotechnological formulation of exploitation and extract aspects such as biologically active substance.Multiple biologically active substances such as many little algae rich in proteins, polysaccharide, grease, carotenoid and unsaturated fatty acids are human important Biological resources storehouses.Algae resource has become food, medicine, the important treasure-house of bioenergy product.Little algal biomass is the good raw material of producing biologically active substance, biomaterial and biofuel.Utilize little algal biomass resource such as little algae or engineering microalgae to produce fossil substitution of resources product, biofuel particularly, can be the bioenergy industry and open up a new technology path, can effectively solve the raw material bottleneck of biofuel industry, for the health of biofuel industry, stable and Sustainable development provide new thinking, thereby realize substituting and continuous development of society economy of fossil resource.At present.The research focus in the whole world all begins to concentrate on little this technology of algae biorefinery.Little algae biorefinery technology promptly refers to based on photosynthesis, cultivates by the bioreactor mass-producing of microalgae cell, produces a kind of technology of biomass fuel and bio-active products
The global research and development tide of little algae arrives, but the R﹠D work of countries in the world in this field also rests on the starting stage of experimental study and pilot scale demonstration basically, and scale preparation still is unrealized.This mainly is that wherein the development as the high-efficiency photobioreactor of studying carrier is one of main bottleneck because the technological difficulties that exist in little algae R﹠D process are more, so this direction is that little algae is cultivated and little algae fixation of C O always
2The Study on Technology focus.
The bioreactor objective function of optimization design relates generally to four aspects: the specific surface area of (1) augmenting response device.The form of bioreactor is various, has openly, and closed (main tube-separating type, board-like and bevel-type three classes) is also arranged, and wherein open specific surface area is bigger; (2) strengthen gas-liquid mass transfer efficient.Generally improve gas-liquid mass transfer effect in little algae culturing process, as gas liquid exchanger, condenser, ultra-filtration equipment, mixing apparatus and nutraceutical feeding mechanism by designing attached device architecture; (3) provide high efficiency light source.Studies show that in a large number little algae absorbs CO
2Produce O
2Speed largely depends on illumination condition.Improving illumination condition need solve two problems, and one is that high efficiency light source provides, and another is the effective distribution of light in reactor.Except utilizing sunlight and common luminescent lamp, also developed and utilized light emitting diode, photoconductive fiber and photoflash lamp various bioreactors as light source as the light source.The position of light source can be divided into external placed type and built-in two kinds, and the built-in distribution that can significantly improve light improve the light source utilization ratio, but cost of manufacture is higher.In addition, various illumination operational conditions as light quality, light intensity, Light To Dark Ratio and light application time etc., also have a significant impact micro algae growth; (4) improve the light transfer efficiency.Discover that simple raising light intensity can not correspondingly improve the product O of little algae
2Speed.With Chlorella kessleri is the algae kind, utilizes the bioreactor of photodiode as light source, when frond density reaches 5 * 10
8During cells/mL, O
2Clean generation speed is 10mmol O
2/ (Lh).When frond was increased to certain density, its apparent product oxygen speed (OPR) began to descend, and if masking phenomenon each other not between the frond in the bioreactor, O
2Throughput rate will improve 5 times at least.
Have more technological difficulties in little algae R﹠D process, it is basic as research wherein to lack bioreactor efficient, energy-conservation, technology maturation, and it has limited little algae research and development and large-scale development greatly.This mainly is because the microalgae photobiological reactor design relates to more multidisciplinary intersection, and the background science principle and the rule of its design systematically do not understood and grasped in present research from profound level, therefore is difficult to form the core technology that solves key issue.Develop low cost, efficient, energy-conservation microalgae photobiological reactor design technology, will lay a solid foundation for the research of little algae biotechnology undoubtedly, hold the bioeconomic initiative of following little algae.
The utility model content
Technical problem to be solved in the utility model provides many groups bubbling style bioreactor that a kind of layout is succinct, practical, be used for small-scale production, to fill the domestic gaps.
For solving the problems of the technologies described above, the technical solution adopted in the utility model is as follows:
A kind of many group bubbling style bioreactors, it comprises casing, bubbling style optical-biological reaction unit, main gas tube, light source and Controlling System, plural bubbling style optical-biological reaction unit in a row places in the casing, main gas tube is connected by the gas distributor of aspiration tube in gas distribution arm and the bubbling style optical-biological reaction unit and aspiration tube bottom, light source places the unitary both sides of every row's bubbling style optical-biological reaction, and Controlling System comprises temperature controlling system and the light-source control system intensity in order to intravital temperature of controlling box and light source.
Wherein, casing is provided with openable lid.
Wherein, cabinet wall scribbles light reflecting material.
Wherein, be equipped with a main gas tube on each top, row's bubbling style optical-biological reaction unit.
Wherein, each root gas distribution arm is provided with gas meter.
Wherein, described bubbling style optical-biological reaction unit is made by high light transmissive material, and the lower end circular arc is received the end, closes up and polish in the upper end, with the soft rubber ball sealing, reserves inlet mouth, air outlet, material-feeding port and electrode socket on the soft rubber ball.
Wherein, the wavelength of light source disposes as requested, and wave band is any wave band among the 250nm-780nm.
Wherein, light source is a tubular lamp, from the bubbling style optical-biological reaction unitary near the position, upper edge near many tubular lamps of the even parallel distribution of bottom position.
Wherein, the light source between adjacent two row's bubbling style optical-biological reaction unit is single or double.
Wherein, be provided with a baffle plate between adjacent two row's bubbling style optical-biological reaction unit, baffle plate is divided into multicell with casing, and baffle surface scribbles light reflecting material.
Beneficial effect: the relative prior art of the utility model has following advantage and effect:
1, adopts the body structure of particular design.This device box inwall scribbles light reflecting material, can prevent effectively that light from leaking, and increases intensity of illumination, improves the efficiency of light energy utilization.Folding design sealing rationally can effectively be guaranteed effective control of temperature and luminous energy.
2, adopt microcomputer-recognized intelligent automatic light source Controlling System.This device the Lights section automatic control system is controlled intensity of illumination by regulating bright lamp quantity, and can realize the multistage light source control by control, secretly circulates as light, and the circulation of different light intensity is switched, and also can replace according to the requirement of light source wave band.
3, light source, bio-reactor team design.The independent cabin of this device reaction device part.Can realize the independent component air feed and the light intensity control in cabin.In addition, can realize the Ventilation Rate control of independent light bio-reactor.Simple for structure, easy to operate, temperature controllable, light intensity, light quality, Ventilation Rate, gaseous fraction, but batch processing.
4, the intensity of light source and light quality are controlled.This illumination cultivation system source adopts can change design, can change light source according to the growing state of different algae kinds and little algae.This device the Lights section can dispose the light source of different optical bands according to the biomass required light quality of growing, and optical band can be from infrared to any wave band of near ultraviolet.
5, temperature intelligent is controlled.This illumination cultivation system contains the multi-point temp sensing, and refrigeration compressor, electro-thermal heating system have realized that the light temperature in the illumination cultivation system is accurately controlled.
Description of drawings
Fig. 1 is the main TV structure synoptic diagram of many group bubbling style bioreactors of the present utility model.
Fig. 2 is the plan structure synoptic diagram of many group bubbling style bioreactors of the present utility model.
Fig. 3 is the left TV structure synoptic diagram of many group bubbling style bioreactors of the present utility model.
Fig. 4 is many rows light source mode of many group bubbling style bioreactors of the present utility model.
Fig. 5 is a kind of mode that many group bubbling style bioreactors of the present utility model use baffle plate.
Fig. 6 is the another kind of mode that many group bubbling style bioreactors of the present utility model use baffle plate.
Embodiment
According to following embodiment, can understand the utility model better.Yet, those skilled in the art will readily understand that embodiment is described only to be used to illustrate the utility model, and should also can not limit the utility model described in detail in claims.
Embodiment 1:
Fig. 1 is the main TV structure synoptic diagram of many group bubbling style bioreactors of the present utility model, and Fig. 2 is its plan structure synoptic diagram, and Fig. 3 is its left TV structure synoptic diagram.Can clearly understanding be arranged to apparatus structure of the present utility model by Fig. 1～3.Many group bubbling style bioreactors of the present utility model mainly comprise casing 1, bubbling style optical-biological reaction unit 2, main gas tube 3, light source 4 and Controlling System 5.
Several bubbling style optical-biological reaction unit 2 in a row place in the casing 1, be equipped with a main gas tube 3 on each 2 top, row's bubbling style optical-biological reaction unit, the gas distributor 7 of main gas tube 3 by aspiration tube 6 in each root gas distribution arm 12 and each bubbling style optical-biological reaction unit and aspiration tube bottom is connected and carries out bubbling, gas distributor 7 adopts the alumina high temperature calcining to form or macromolecular material is made, mixed gas is distributed in the nutrient solution with atomic little bubble, for can the unitary gas flow of each optical-biological reaction of independent control, on each root gas distribution arm 6, and be positioned at 2 tops, bubbling style optical-biological reaction unit and be provided with gas meter 9.The setting of gas meter is to establish for independent pilot-gas flow; also can directly use valve control; valve or gas meter are set to control the gas flow rate of optical-biological reaction in a row unit 2 simultaneously on main gas tube 3; valve or gas meter perhaps are set on the portion gas distribution arms do not influence essence of the present utility model with the gas flow rate of control section optical-biological reaction unit 2; all within protection domain of the present utility model; any valve or gas meter even are not set; optical-biological reaction unit 2 in the whole box body all adopts identical gas flow rate, also within protection domain of the present utility model.Main gas tube 3 external airing systems, airing system is made up of source of the gas, gas mixer, buffer container, strainer, gas meter, and those skilled in the art can realize the assembling of airing system according to prior art.
Above-mentioned bubbling style optical-biological reaction unit 2 is made by high light transmissive material, as high-boron-silicon glass.Its lower end circular arc is received the end, closes up and polish in the upper end, with soft rubber ball 10 sealings, reserves inlet mouth, air outlet, material-feeding port and electrode socket (as temperature electrode or pH electrode) on the soft rubber ball.The utility model to the quantity of bubbling style optical-biological reaction unit 2, size, arrangement mode without any restriction; those skilled in the art can be according to the cultivation demand and the needed industrial scale of different microalgae; the adaptive change that bubbling style optical-biological reaction unit 2 is carried out in shape; select the optical-biological reaction unit of suitable dimension specification; determine suitable element number; the setting of arranging; and determine the size of adaptive casing, above-mentioned variation is all within protection domain of the present utility model.
Casing 1 is provided with openable lid 8, makes things convenient for the taking-up of bubbling style optical-biological reaction unit 2 to insert, and also makes things convenient for the replacing of light source 4, and the utility model is not done any restriction to the position and the size of lid, can be arranged on the top of the sidewall or the casing of casing.For the ease of observing the response situation of casing 1 inside, also a viewing window can be set on casing 1.Casing 1 inwall scribbles light reflecting material, realizes that luminous energy effectively utilizes.Casing can adopt double-layer sealing structure, to keep temperature in the casing.Casing 1 leaves inlet pipe road junction and escape pipe road junction usually.
Controlling System 5 comprises temperature controlling system and the light-source control system intensity in order to intravital temperature of controlling box and light source.Temperature controlling system comprises: refrigeration compressor, blower fan electric fan, electric heating system, multi-point temp transmitter, micro computer temperature control system.Those skilled in the art can manifest the intravital Controllable Temperature of case according to prior art.Light-source control system 5 for example can be realized the controlled of intensity of illumination by the bright lamp quantity of control fluorescent tube by microcomputer-recognized intelligent control lighting system, and the time circulation can also be set, and allows light source produce illumination in certain period, extinguishes in certain period.Those skilled in the art can work out light level according to prior art.
As an optimal way of the present utility model, can between adjacent two row's bubbling style optical-biological reaction unit 2, baffle plate 11 be set, baffle plate 11 surfaces scribble light reflecting material, and baffle plate 11 is divided into multicell with casing 1, realizes that the intensity of light source of each chamber is independent.The utility model is the quantity of defining baffle not, can divide how many individual independently chambers as required, the baffle plate of respective numbers is set, but for example shown in the image pattern 5, between all adjacent two row's bubbling style optical-biological reaction unit 2, baffle plate is set, makes each row's bubbling style optical-biological reaction unit 2 all form independently space, realize the independence of intensity of illumination, shown in also can image pattern 6, between some adjacent two row's bubbling style optical-biological reaction unit 2, baffle plate be set, casing is divided into two independently chambers.Whether baffle plate is set is not conflicted with row's number of light source, for example can adopt the mode that two row's light sources are set between adjacent two row's bubbling style optical-biological reaction unit 2, baffle plate is inserted between two row's light sources (Fig. 5), also can adopt the mode that single light source is set between adjacent two row's bubbling style optical-biological reaction unit 2, baffle plate is inserted between single light source and the optical-biological reaction unit, all has light source (Fig. 6) as long as guarantee each chamber of dividing.The utility model is not done any restriction to the fixed form of baffle plate; can adopt casing draw-in groove fixed dam; perhaps support fixed dam; perhaps baffle plate being fixed on the first-class mode of light source can; even do not adopt any fixed form; directly baffle plate is inserted between light source and the light source or between light source and the optical-biological reaction unit, also at protection domain of the present utility model and so on.
Can realize the control of temperature in the culturing process, light intensity, light quality, Ventilation Rate, gaseous fraction by many group bubbling style bioreactors of the present utility model.
Embodiment 2:
Cultivate chlorella at many groups bubbling style bioreactor that embodiment 1 describes, with bubbling style optical-biological reaction unit (diameter 10cm, high be 60cm) in the f/2 substratum of packing into, casing is sterilized outward, inserts chlorella, inoculum size is 0.2g/L, reinstall in the casing, main gas tube, gas distribution arm are connected with gas distributor with aspiration tube, plug temperature electrode or pH electrode, plug the air outlet, the jam-pack soft rubber ball.Open air distributing device,, open the valve on the gas distribution arm, bubbling in the bubbling style optical-biological reaction unit by the ratio of gas meter control carbonic acid gas and air.As shown in Figure 5, the control intensity of illumination in four chambeies of reactor is followed successively by 3000lux, 5000lux, 70000lux, and 9000lux, spin manifold temperature are controlled at 26 ℃, CO
2Ventilation content be respectively 1% (v/v), 3% (v/v), 5% (v/v), 7% (v/v), cultivated for two weeks after, biomass is up to 2.253g/L, fat content is 35%.Experiment is in time cleaned bubbling style optical-biological reaction unit after finishing, and waits until next time and uses.
Claims (10)
1. organize the bubbling style bioreactor one kind more, it is characterized in that it comprises casing (1), bubbling style optical-biological reaction unit (2), main gas tube (3), light source (4) and Controlling System (5), plural bubbling style optical-biological reaction unit (2) in a row places in the casing (1), main gas tube (3) is connected by the aspiration tube (6) in gas distribution arm (12) and the bubbling style optical-biological reaction unit and the gas distributor (7) of aspiration tube bottom, light source (4) places the both sides of every row's bubbling style optical-biological reaction unit (2), and Controlling System (5) comprises temperature controlling system and the light-source control system intensity in order to intravital temperature of controlling box and light source.
2. many group bubbling style bioreactors according to claim 1 is characterized in that casing (1) is provided with openable lid (8).
3. many group bubbling style bioreactors according to claim 1 is characterized in that casing (1) inwall scribbles light reflecting material.
4. many group bubbling style bioreactors according to claim 1 is characterized in that being equipped with a main gas tube (3) on each top, row bubbling style optical-biological reaction unit (2).
5. many group bubbling style bioreactors according to claim 1 is characterized in that on each root gas distribution arm (6) gas meter (9) being housed.
6. many group bubbling style bioreactors according to claim 1, it is characterized in that described bubbling style optical-biological reaction unit (2) made by high light transmissive material, the lower end circular arc is received the end, close up and polish in the upper end, with soft rubber ball (10) sealing, reserve inlet mouth, air outlet, material-feeding port and electrode socket on the soft rubber ball.
7. many group bubbling style bioreactors according to claim 1, the wavelength that it is characterized in that light source (4) is 250nm～780nm.
8. many group bubbling style bioreactors according to claim 1 is characterized in that light source (4) be a tubular lamp, from bubbling style optical-biological reaction unit (2) near the position, upper edge extremely near many tubular lamps of the even parallel distribution of bottom position.
9. according to claim 1 or 8 described many group bubbling style bioreactors, it is characterized in that the light source (4) between the adjacent two row bubbling style optical-biological reaction unit (2) is single or double.
10. many group bubbling style bioreactors according to claim 1, it is characterized in that being provided with a baffle plate (11) between the adjacent two row bubbling style optical-biological reaction unit (2), baffle plate (11) is divided into multicell with casing (1), and baffle plate (11) surface scribbles light reflecting material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201020282646.0U CN201737931U (en) | 2010-08-04 | 2010-08-04 | Multi-group bubbling type photobioreactor |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201020282646.0U CN201737931U (en) | 2010-08-04 | 2010-08-04 | Multi-group bubbling type photobioreactor |
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| CN201737931U true CN201737931U (en) | 2011-02-09 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101935610A (en) * | 2010-08-04 | 2011-01-05 | 南京工业大学 | Multi-group bubbling type photobioreactor |
| CN103476245A (en) * | 2011-02-11 | 2013-12-25 | L格姆有限公司 | Method and bioreactor for the cultivation of microorganisms |
| CN104560703A (en) * | 2013-10-15 | 2015-04-29 | 纳米及先进材料研发院有限公司 | Photobioreactor system for air purification |
| CN105483001A (en) * | 2014-12-16 | 2016-04-13 | 纳米及先进材料研发院有限公司 | Photobioreactor system used for air purification |
| CN108823075A (en) * | 2018-06-06 | 2018-11-16 | 重庆大学 | A kind of photo-biological reactor that can effectively absorb and utilize tail gas |
-
2010
- 2010-08-04 CN CN201020282646.0U patent/CN201737931U/en not_active Expired - Fee Related
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101935610A (en) * | 2010-08-04 | 2011-01-05 | 南京工业大学 | Multi-group bubbling type photobioreactor |
| CN101935610B (en) * | 2010-08-04 | 2013-05-01 | 南京工业大学 | Multi-group bubbling type photobioreactor |
| CN103476245A (en) * | 2011-02-11 | 2013-12-25 | L格姆有限公司 | Method and bioreactor for the cultivation of microorganisms |
| CN104560703A (en) * | 2013-10-15 | 2015-04-29 | 纳米及先进材料研发院有限公司 | Photobioreactor system for air purification |
| CN105483001A (en) * | 2014-12-16 | 2016-04-13 | 纳米及先进材料研发院有限公司 | Photobioreactor system used for air purification |
| CN108823075A (en) * | 2018-06-06 | 2018-11-16 | 重庆大学 | A kind of photo-biological reactor that can effectively absorb and utilize tail gas |
| CN108823075B (en) * | 2018-06-06 | 2020-06-23 | 重庆大学 | Photo-biological reaction device capable of effectively absorbing and utilizing tail gas |
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