CN103205361A - Aerosol microalgae illuminating reaction device - Google Patents
Aerosol microalgae illuminating reaction device Download PDFInfo
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- CN103205361A CN103205361A CN2013101393785A CN201310139378A CN103205361A CN 103205361 A CN103205361 A CN 103205361A CN 2013101393785 A CN2013101393785 A CN 2013101393785A CN 201310139378 A CN201310139378 A CN 201310139378A CN 103205361 A CN103205361 A CN 103205361A
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Abstract
The invention belongs to the technical field of biological reaction devices, and relates to an aerosol microalgae illuminating reaction device. An upper cover is fixedly arranged at the top end of a stainless steel tank body. An end socket is arranged between the upper cover and the stainless steel tank body. A feed inlet and an air outlet are respectively arranged on the side face outside the top of the stainless steel tank body. A sensor interface is arranged on the lower side on the middle part on the side face of the stainless steel tank body corresponding to the feed inlet. Three observing holes are arranged on the side face of the stainless steel tank body. A discharge port is fixedly arranged in the center at the bottom of the stainless steel tank body. The bottom of the stainless steel tank body is fixedly installed on a fixed bracket. A snakelike heating coil is arranged on the periphery of a middle section in a cavity of the stainless steel tank body. Light cross brackets are respectively fixed at the upper and lower ends of the cavity of the snakelike heating coil. Light crosses are vertically fixed on the circumference of the light cross brackets uniformly. An aerosol generator is fixedly arranged at the bottom of the cavity of the stainless steel tank body. The device is simple in structure, strong in universality, simple and convenient to use and operate, good in fermenting effect and environment-friendly.
Description
Technical field:
The invention belongs to biologic reaction equipment manufacturing technology field, relate to a kind of jumbo aerosol and decline algae LED built in light according to the reactor, particularly a kind of aerosol of the structure algae illumination reaction device that declines.
Background technology:
Bio-reactor is the important means of production that uses in the biotechnology, and the design of novel reactor often can promote developing rapidly of an industry.The development and use industry of little algae is fast-developing at present new industry, various little algae can provide novel quality protein product, healthcare product, pharmaceutical prod and energy product etc., but micro-algae large-scale cultivation at present mainly is to carry out in the runway pond of open type, its shortcoming is a lot: the one, and the runway pond is difficult to avoid bacterium and fungi, other speed is given birth to assorted algae and protozoic pollution and predation, has a strong impact on output; The 2nd, because seasonal climate and wait the variation of natural condition to the influence of output round the clock; The 3rd, the runway pond will take a large amount of soils, is subject to the influence of surrounding environment; The 4th, cultivate in large-scale runway pond, owing to illumination, temperature etc. are non-constant, manages very difficult; These shortcomings become the restraining factors that little algae is further enhanced production capacities and industrialization is produced, and existing totally-enclosed bioreactor mainly contains column, the flat and middle-size and small-size reactor of tubular type, is difficult to reach the requirement of industrialization.Therefore, seek to design a kind of enclosed illumination bio-reactor of the big capacity of industrialization that is applied to and to become one of essential condition of micro-algae large-scale production and development and use.
Summary of the invention:
The objective of the invention is to overcome the shortcoming that prior art exists, seek a kind of aerosol of design preparation algae illumination reaction device that declines, single unit system adopts the flexible stirring technique of the aerosol of full-closed structure and built-in led light source, be combined into one the formula reactor, be applied to marine microalgae biological respinse occasion.
To achieve these goals, agent structure of the present invention comprises that stainless steel tank body, vision slit, end socket, loam cake, opening for feed, sensor interface, air outlet, discharge port, light intensity controller, power lead, snakelike heating coil, aerosol generator, light beam, light beam support, LED lead, upper cover plate, light beam penetrate hole, central hand-hole, cross frame, ring support and anchor; The top end of the stainless steel tank body of hollow type cylindrical structure fixedly is shaped on loam cake, be shaped on end socket between loam cake and the stainless steel tank body, be shaped on opening for feed and air outlet on the top outside of stainless steel tank body respectively, middle part, the side downside of the stainless steel tank body of corresponding opening for feed is shaped on sensor interface, is used for the external sensor lead; The evenly distributed vision slit that is shaped on three same structures about on the side of stainless steel tank body, the bottom center place of stainless steel tank body fixedly is shaped on discharge port, and the bottom overhead type of stainless steel tank body is fixedly installed on the anchor; The inner chamber interlude week side circulating type of stainless steel tank body is shaped on the snakelike heating coil of spirane structure, the inner chamber upper and lower ends of snakelike heating coil fixedly is shaped on the light beam support of two symmetrical structures respectively, the evenly distributed light beam that vertically fixedly is shaped on the light beam support middle circle contour of annulus disk-like structure, the top of each light beam is communicated with the light intensity controller optoelectronic information by the LED lead that is communicated with respectively, and connect external power by light intensity controller through the photovoltaic cell power lead, realize that photoelectricity is communicated with and the luminous intensity of control light beam; The inner chamber inner bottom part of stainless steel tank body fixedly is shaped on aerosol generator; End socket comprises that upper cover plate, light beam penetrate hole and central hand-hole, and the end socket center of ring texture hollows out formula and is shaped on central hand-hole and inserts for the photovoltaic cell power lead, is shaped on the light beam that fixing light beam uses on the middle circle contour of the cover plate of ring texture respectively and penetrates the hole; The profile of light beam support and end socket and symmetrical configuration, the light beam support interstitial chiasma of skeleton construction is shaped on cross frame, and the circle center place is shaped on the ring support that is connected with cross frame, and evenly distributing on the ring support is fixedly equipped with light beam.
The profile of the stainless steel tank body of the illumination reaction device that the present invention relates to is the vertical type circular cylinder body, and diameter and aspect ratio are 1:2.5, and physical construction is vessels not under pressure, and the cylindrical shell material is stainless steel, carbon steel sheet, plastics or skeleton construction; Light beam adopts the LED diode to be connected into light belt and inserts in the transparent glass tube, the Glass tubing lower end closed, and the upper end is the LED wire outlet, after installing, prevents water seal, constitutes totally enclosed light beam formula light source; Light beam is the vertical type circular arrangement in stainless steel tank body, make between light beam and stainless steel tank body inwall and light beam and the light beam equidistantly, and intensity of illumination is evenly distributed in reaction unit; Intensity of illumination in the reaction unit is 500Lx-15000Lx, convertible and adjusting; Illumination colourity can be regulated according to the requirement of different microalgae, or increases blueness or red light source; Energy of light source adopts photovoltaic solar cell to supply with, or adopts civil power to replenish.
The aerosol stream nature that alr mode of the present invention adopts aerosol generator to emit rises, and convection current realizes the effect of stirring, mass transfer and heat transfer about the drive nutrient solution; The micropore air outlet of aerosol generator is installed in the bottom, the gas supply of aerosol generator can divide external source air feed and internal recycle air feed, the external source air feed is provided by aerator pump, and the internal recycle air feed provides source of the gas by the air outlet on stainless steel tank body top, enters internal recycling after pumping into aerosol generator; After gas pumps into aerosol generator, emit the formation mist flow from the micropore of its micro-pore aeration atomizer and drive current downflow realization stirring on the fermented liquid; Can oxygenation or carbonating when aerosol generator realize to stir or fill nitrogen, or air feed in parallel with carbonic acid gas, its utilization ratio is 5 times of conventional air feed; Adopt the normal temperature chemical disinfection in the whole fermentation reaction process; With the thermostat(t)ed water circulation, keep homo(io)thermism in the stainless steel tank body in the snakelike heating coil of placing in the stainless steel tank body, stainless steel tank body outer wall or be incubated processing.
Auxiliary design of the present invention has feed supplement jar, acid jar, alkali jar, sterile air and carbonic acid gas, looks the demand of cultivating different microalgae and increases and decreases.
The present invention compared with prior art, the one, adopt full-closed structure, avoid the influence of extraneous factor, parameters such as its illumination, temperature, PH, dissolved oxygen, nutritive salt freely are controlled, and avoid bacterium, mix algae, protozoic pollution; The 2nd, but built-in light source focus utilization luminous energy all utilizes light source, avoids light source diffusion or light pollution; The 3rd, the flexible spraying of aerosol is stirred, and inflatablely unites two into one with stirring, and it is simple in structure, and cost is lower, and highly versatile uses easy and simple to handlely, and ferment effect is good, environmental friendliness.
Description of drawings:
Fig. 1 is main body contour structures principle schematic of the present invention.
Fig. 2 is body interior structural principle synoptic diagram of the present invention.
Fig. 3 is the end enclosure structure principle schematic that the present invention relates to.
Fig. 4 is the light beam supporting structure principle schematic that the present invention relates to.
Embodiment:
Also be described further by reference to the accompanying drawings below by embodiment.
Embodiment:
The agent structure of present embodiment comprises that stainless steel tank body 1, vision slit 2, end socket 3, loam cake 4, opening for feed 5, sensor interface 6, air outlet 7, discharge port 8, light intensity controller 9, power lead 10, snakelike heating coil 11, aerosol generator 12, light beam 13, light beam support 14, LED lead 15, upper cover plate 16, light beam penetrate hole 17, central hand-hole 18, cross frame 19, ring support 20 and anchor 21; The top end of the stainless steel tank body 1 of hollow type cylindrical structure fixedly is shaped on loam cake 4, be shaped on end socket 3 between loam cake 4 and the stainless steel tank body 1, be shaped on opening for feed 5 and air outlet 7 on the top outside of stainless steel tank body 1 respectively, middle part, the side downside of the stainless steel tank body 1 of corresponding opening for feed 5 is shaped on sensor interface 6, is used for the external sensor lead; The evenly distributed vision slit 2 that is shaped on three same structures about on the side of stainless steel tank body 1, the bottom center place of stainless steel tank body 1 fixedly is shaped on discharge port 8, and the bottom overhead type of stainless steel tank body 1 is fixedly installed on the anchor 21; The inner chamber interlude week side circulating type of stainless steel tank body 1 is shaped on the snakelike heating coil 11 of spirane structure, the inner chamber upper and lower ends of snakelike heating coil 11 fixedly is shaped on the light beam support 14 of two symmetrical structures respectively, the evenly distributed light beam 13 that vertically fixedly is shaped on the light beam support 14 middle circle contours of annulus disk-like structure, the top of each light beam 13 is communicated with light intensity controller 9 optoelectronic informations by the LED lead 15 that is communicated with respectively, and pass through light intensity controller 9 through photovoltaic cell power lead 10 connection external powers, realize that photoelectricity is communicated with and the luminous intensity of control light beam 13; The inner chamber inner bottom part of stainless steel tank body 1 fixedly is shaped on aerosol generator 12; End socket 3 comprises that upper cover plate 16, light beam penetrate hole 17 and central hand-hole 18, end socket 3 centers of ring texture hollow out formula and are shaped on central hand-hole 18 for the access of photovoltaic cell power lead, and being shaped on fixedly on the middle circle contour of the cover plate 16 of ring texture respectively, the light beam of light beam 13 usefulness penetrates hole 17; Profile and the symmetrical configuration of light beam support 14 and end socket 3, light beam support 14 interstitial chiasma of skeleton construction are shaped on cross frame 19, and the circle center place is shaped on the ring support 20 that is connected with cross frame 19, and evenly distributing on the ring support 20 is fixedly equipped with light beam 13.
Present embodiment when fermentation, earlier to after stainless steel tank body 1 sterilization, from opening for feed 5 pump into cultivate the routine that little algae uses filtered water to constant volume, add chemostefilant; The aerosol generator 12 of opening stirring system again stirred 5 minutes, chemostefilant is stirred after, shut down and closed all each jar mouth of connecting with stainless steel tank body 1 outward static 12 hours; Add the residual poison scavenging agent from opening for feed 5 again, opened air outlet 7 and aerosol generator 12 strong aerations 10 minutes, after the thorough removing of residual poison material or neutralization were lost efficacy, the nutrient chemical that adds the process high-temperature sterilization stirred, and opens light intensity controller 9 simultaneously and makes the nutrient chemical temperature regulation to preset temp; After inserting the algae kind, opening for feed 5 closes opening for feed 5 then; Open aerosol generator 12 and air outlet 7 again, be adjusted to little mist air input and give vent to anger the arrival balance, make a little higher than ambient pressure of stainless steel tank body 1 internal gas pressure; Open light source at last and make intensity of illumination be adjusted to default intensity, finish the sterilization inoculation, enter little algae breeding phase; At little algae breeding phase, little algae cultivation period is longer, and the reply parameters enters adjusting; Because day and night temperature and Changes in weather, 9 pairs of temperature of available light intensity controller are regulated; Because the growth and breeding of little algae, frond increases, based on the interception fine setting increase intensity of illumination of frond; Photosynthesis in the culturing process causes the consumption of carbonic acid gas, increases carbonic acid gas from aerosol generator 12; The variation of PH is regulated with the auxilliary soda acid jar of establishing; Nutraceutical consumption is by the agent of feed supplement jar Ensure Liquid; Be adjusted to the optimal culture conditions that little algae is cultivated, early stage at the vegetative cell logarithmic growth when Haematocoocus Pluvialls is cultivated, add intense light irradiation, regulate the nutrient chemical prescription when increasing temperature and feed supplement, increase the accumulation of astaxanthin for Haematocoocus Pluvialls; When frond grows to default biomass or active substance and reaches predetermined amount, open discharge port 5 dischargings, cultivating process finishes.
Claims (4)
1. aerosol algae illumination reaction device that declines is characterized in that agent structure comprises that stainless steel tank body, vision slit, end socket, loam cake, opening for feed, sensor interface, air outlet, discharge port, light intensity controller, power lead, snakelike heating coil, aerosol generator, light beam, light beam support, LED lead, upper cover plate, light beam penetrate hole, central hand-hole, cross frame, ring support and anchor; The top end of the stainless steel tank body of hollow type cylindrical structure fixedly is shaped on loam cake, be shaped on end socket between loam cake and the stainless steel tank body, be shaped on opening for feed and air outlet on the stainless steel tank body top outside respectively, middle part, the stainless steel tank body side downside of corresponding opening for feed is shaped on sensor interface, is used for the external sensor lead; The evenly distributed vision slit that is shaped on three same structures about on the stainless steel tank body side, stainless steel tank body bottom center place fixedly is shaped on discharge port, and stainless steel tank body bottom overhead type is fixedly installed on the anchor; The inner chamber interlude circulating type of stainless steel tank body is shaped on the snakelike heating coil of spirane structure, the inner chamber upper and lower ends of snakelike heating coil fixedly is shaped on the light beam support of two symmetrical structures respectively, the evenly distributed light beam that vertically fixedly is shaped on the light beam support middle circle contour of circular ring, each light beam top is communicated with the light intensity controller optoelectronic information by the LED lead that is communicated with respectively, and connect external power by light intensity controller through the photovoltaic cell power lead, realize that photoelectricity is communicated with and the luminous intensity of control light beam; The inner chamber inner bottom part of stainless steel tank body fixedly is shaped on aerosol generator; End socket comprises that upper cover plate, light beam penetrate hole and central hand-hole, and the end socket center of ring texture hollows out formula and is shaped on central hand-hole and inserts for the photovoltaic cell power lead, is shaped on the light beam that fixing light beam uses on the middle circle contour of the cover plate of ring texture respectively and penetrates the hole; The profile of light beam support and end socket and symmetrical configuration, the light beam support interstitial chiasma of skeleton construction is shaped on cross frame, and the circle center place is shaped on the ring support that is connected with cross frame, and evenly distributing on the ring support is fixedly equipped with light beam.
2. the aerosol according to claim 1 algae illumination reaction device that declines, the profile that it is characterized in that stainless steel tank body is the vertical type circular cylinder body, diameter and aspect ratio are 1:2.5, and physical construction is vessels not under pressure, and the cylindrical shell material is stainless steel, carbon steel sheet, plastics or skeleton construction; Light beam adopts the LED diode to be connected into light belt and inserts in the transparent glass tube, the Glass tubing lower end closed, and the upper end is the LED wire outlet and carries out the waterproof sealing; Light beam is the vertical type circular arrangement in stainless steel tank body, equidistantly, intensity of illumination evenly distributes in reaction unit between light beam and stainless steel tank body inwall and light beam and the light beam; Intensity of illumination in the reaction unit is 500Lx-15000Lx, can change and regulate; Illumination colourity can be regulated according to the requirement of different microalgae, or increases blueness or red light source; Energy of light source adopts photovoltaic solar cell to supply with or adopt civil power to replenish.
3. the aerosol according to claim 1 algae illumination reaction device that declines is characterized in that the aerosol stream nature that alr mode adopts aerosol generator to emit rises, drive nutrient solution up and down convection current realize the effect of stirring, mass transfer and heat transfer; The micropore air outlet of aerosol generator is installed in the bottom, the gas supply of aerosol generator divides external source air feed and internal recycle air feed, the external source air feed is provided by aerator pump, and the internal recycle air feed provides source of the gas by the air outlet on stainless steel tank body top, enters internal recycling after pumping into aerosol generator; After gas pumps into aerosol generator, emit the formation mist flow from the micropore of its micro-pore aeration atomizer and drive current downflow realization stirring on the fermented liquid; Can oxygenation or carbonating when aerosol generator realize to stir or fill nitrogen, or air feed in parallel with carbonic acid gas, its utilization ratio is 5 times of conventional air feed; Adopt the normal temperature chemical disinfection in the whole fermentation reaction process; With the thermostat(t)ed water circulation, keep homo(io)thermism in the stainless steel tank body in the snakelike heating coil of placing in the stainless steel tank body, stainless steel tank body outer wall or be incubated processing.
4. the aerosol according to claim 1 algae illumination reaction device that declines is characterized in that the auxiliary design comprises feed supplement jar, acid jar, alkali jar, sterile air and carbonic acid gas, and the demand of looking the cultivation different microalgae increases and decreases.
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Cited By (8)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103525686A (en) * | 2013-10-24 | 2014-01-22 | 重庆大学 | Composite reinforced microalgae photobioreactor based on hollow light pipes |
CN104073435A (en) * | 2014-07-02 | 2014-10-01 | 杭州鑫伟低碳技术研发有限公司 | Movable all-weather microalgae light complement bioreaction culture system and culture method |
CN105331530A (en) * | 2014-08-17 | 2016-02-17 | 王金鹤 | Novel biological detection device |
CN107460115A (en) * | 2017-07-24 | 2017-12-12 | 大连理工大学 | It is sustained feed supplement bioreactor and microalgae culture method |
CN108179100A (en) * | 2018-03-26 | 2018-06-19 | 尹康康 | A kind of photosynthetic bacteria screening and culturing method |
CN108410723A (en) * | 2018-03-26 | 2018-08-17 | 尹康康 | A kind of photosynthetic bacteria culture systems |
CN109370899A (en) * | 2018-12-17 | 2019-02-22 | 山东理工大学 | A kind of automation microalgae complex light high-efficient culture device |
CN113136340A (en) * | 2020-01-19 | 2021-07-20 | 中国石油化工股份有限公司 | Photobioreactor, method for culturing photosynthetic microorganisms and application of photosynthetic microorganisms |
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Cited By (12)
Publication number | Priority date | Publication date | Assignee | Title |
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CN103525686A (en) * | 2013-10-24 | 2014-01-22 | 重庆大学 | Composite reinforced microalgae photobioreactor based on hollow light pipes |
CN103525686B (en) * | 2013-10-24 | 2015-01-21 | 重庆大学 | Composite reinforced microalgae photobioreactor based on hollow light pipes |
CN104073435A (en) * | 2014-07-02 | 2014-10-01 | 杭州鑫伟低碳技术研发有限公司 | Movable all-weather microalgae light complement bioreaction culture system and culture method |
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CN107460115A (en) * | 2017-07-24 | 2017-12-12 | 大连理工大学 | It is sustained feed supplement bioreactor and microalgae culture method |
CN108179100A (en) * | 2018-03-26 | 2018-06-19 | 尹康康 | A kind of photosynthetic bacteria screening and culturing method |
CN108410723A (en) * | 2018-03-26 | 2018-08-17 | 尹康康 | A kind of photosynthetic bacteria culture systems |
CN108179100B (en) * | 2018-03-26 | 2021-06-08 | 山东百沃生物科技有限公司 | Photosynthetic bacteria screening and culturing method |
CN108410723B (en) * | 2018-03-26 | 2021-08-13 | 上海持美生物环保科技有限公司 | Photosynthetic bacteria culture system |
CN109370899A (en) * | 2018-12-17 | 2019-02-22 | 山东理工大学 | A kind of automation microalgae complex light high-efficient culture device |
CN113136340A (en) * | 2020-01-19 | 2021-07-20 | 中国石油化工股份有限公司 | Photobioreactor, method for culturing photosynthetic microorganisms and application of photosynthetic microorganisms |
CN113136340B (en) * | 2020-01-19 | 2023-05-05 | 中国石油化工股份有限公司 | Photo bioreactor, photosynthetic microorganism culture method and application thereof |
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Application publication date: 20130717 |