CN108410723B

CN108410723B - Photosynthetic bacteria culture system

Info

Publication number: CN108410723B
Application number: CN201810252051.1A
Authority: CN
Inventors: 王玉良; 胡伟; 尹康康; 张家俊; 韩涛
Original assignee: Shanghai Chi Mei Bio Environmental Protection Technology Co ltd
Current assignee: Shanghai Chi Mei bio Environmental Protection Technology Co.,Ltd.
Priority date: 2018-03-26
Filing date: 2018-03-26
Publication date: 2021-08-13
Anticipated expiration: 2038-03-26
Also published as: CN108410723A

Abstract

The invention belongs to the technical field of photosynthetic bacteria culture, in particular to a photosynthetic bacteria culture system, which comprises an illumination module, an incubator, a first controller, a stirring module, an environment adjusting module, a nutrient box and a gas exchange module, wherein the incubator is used for culturing photosynthetic bacteria, the illumination module is positioned at one side of the incubator, the illumination module is internally provided with a circulating pipe, both ends of the circulating pipe are communicated with the incubator, the stirring module is arranged at the upper end of the incubator, the controller is positioned at the upper end of the stirring module, the environment adjusting module is arranged on the incubator, the nutrient box is positioned at the side of the stirring module, the nutrient box is communicated with the incubator, the gas exchange module is positioned at the side of the incubator, and the gas exchange module is communicated with the incubator. Is beneficial to the large-scale culture of the photosynthetic bacteria.

Description

Photosynthetic bacteria culture system

Technical Field

The invention belongs to the technical field of bacterial culture, and particularly relates to a photosynthetic bacteria culture system.

Background

Photosynthetic bacteria are prokaryotes which appear earliest on the earth, commonly exist in the nature and have an original light energy synthesis system, and are microorganisms which take light as energy and can use organic matters, sulfides, ammonia and the like in the nature as hydrogen donor and carbon source to carry out photosynthesis under the anaerobic illumination or aerobic dark condition. Photosynthetic bacteria are widely distributed in soil, paddy fields, swamps, lakes, rivers, seas and the like in the nature, and are mainly distributed in anoxic zones to which light can be transmitted in aquatic environments.

In aquaculture, the photosynthetic bacteria can degrade toxic substances such as nitrite, sulfide and the like in a water body, and realize the functions of serving as bait, purifying water quality, preventing diseases, serving as a feed additive and the like; the photosynthetic bacteria have strong adaptability, can tolerate high-concentration organic wastewater, has certain tolerance and decomposition capacity on toxicants such as phenol, cyanogen and the like, and has strong decomposition and transformation capacity. But the growth of photosynthetic bacteria is mainly affected by temperature, light, pH and oxygen content. In order to realize the large-scale culture of photosynthetic bacteria, at present, the culture of photosynthetic bacteria is mainly carried out by putting a culture medium into a fermentation device and adding strains for culture, and the culture mode has high cost and large equipment investment on the required culture medium; 2. a plastic barrel is adopted, a culture medium and strains are placed in the plastic barrel, sunlight is utilized to provide light energy and heat energy, but the mode is influenced by the external environment and the sedimentation effect of photosynthetic bacteria, so that the stability of a product is poor, and large-scale culture is difficult to realize.

In view of this, the photosynthetic bacteria culture system of the present invention is mainly used for culturing photosynthetic bacteria, and can increase the culture scale of photosynthetic bacteria.

Disclosure of Invention

In order to make up the defects of the prior art, the invention provides a photosynthetic bacteria culture system which is mainly used for culturing photosynthetic bacteria, improves the culture scale of the photosynthetic bacteria and enables the photosynthetic bacteria to be produced in large quantities. The invention can provide a proper growing environment for photosynthetic bacteria through the mutual matching work of the incubator, the controller I, the stirring module, the environment adjusting module, the nutrient box and the gas exchange module, and can automatically compensate the culture of the photosynthetic bacteria through artificial illumination under the condition of insufficient utilization of natural light through the action of the illumination module, thereby being beneficial to the large-scale culture of the photosynthetic bacteria.

The technical scheme adopted by the invention for solving the technical problems is as follows: a photosynthetic bacteria culture system comprises a lighting module, an incubator, a controller I, a stirring module, an environment adjusting module, a nutrient box and a gas exchange module, wherein the incubator is used for culturing photosynthetic bacteria; the illumination module is positioned on one side of the incubator and is used for photosynthesis of photosynthetic bacteria; a circulating pipe is arranged in the illumination module, and both ends of the circulating pipe are communicated with the incubator; the circulating pipe is provided with a first infusion pump, and the first infusion pump is used for conveying liquid in the circulating pipe; the stirring module is arranged at the upper end of the incubator and is used for uniformly stirring the photosynthetic bacteria and the nutrient solution in the incubator; the controller is positioned at the upper end of the stirring module, and is used for adjusting and controlling the stirring module, the gas exchange module, the environment adjusting module and the nutrient box; the environment adjusting module is arranged on the incubator and used for adjusting the environment in the incubator; the nutrient box is positioned beside the stirring module and communicated with the incubator, and the nutrient box is used for temporarily storing nutrient solution and conveying the culture solution into the incubator; the gas exchange module is positioned beside the incubator and communicated with the incubator, and is used for replacing gas in the incubator and removing oxygen in the gas; the upper end of incubator is provided with the fungus liquid inlet port, and the lower extreme of incubator is provided with the fungus liquid and takes out the mouth.

The illumination module comprises an annular lamp tube group, an annular reflector, an annular black ink shading group, a black ink main pipe, a controller II, a valve I, a valve II, a black ink storage tank and an infusion pump II, wherein the annular lamp tube group is a plurality of first lamp tubes which are encircled to form a circle; the annular reflector is positioned on the inner side of the annular lamp tube group and used for reflecting light emitted by the annular lamp tube group; the circulating pipe is circularly wound on the outer wall of the annular light unit; the annular black ink shading group is formed by a plurality of black ink branch pipes which are surrounded into a circle, is sleeved outside the circulating pipe and is used for shading light rays outside the circulating pipe; the black ink main pipe is positioned at the lower end of the annular black ink shading group and is communicated with each black ink branch pipe; the photosensitive sensors are uniformly distributed on the outer wall of the annular black ink shading group and are used for detecting the light intensity outside the annular black ink shading group; the controller II is positioned at the lower end of the annular lamp tube group, and signals are transmitted by the controller II through the photosensitive sensor to control the opening and closing of the annular lamp tube group, the valve I, the valve II, the infusion pump II and the black ink storage tank; the first valve is positioned at the bottom end of the black ink branch pipe and used for controlling the flow direction of black ink in the black ink branch pipe; the second valve is positioned at the bottom end of the black ink main pipe and used for controlling the flow direction of black ink in the black ink main pipe; and the second infusion pump is positioned at the lower end of the second valve and is used for conveying the black ink in the black ink storage tank to the black ink branch pipe. When the culture medium is used, the first infusion pump, the first controller, the second controller, the first valve, the second valve, the photosensitive sensor, the black ink storage tank and the second infusion pump are started, the first infusion pump extracts and conveys the bacterial liquid in the culture box to the circulating pipe and enables the bacterial liquid to flow in the circulating pipe in a circulating mode all the time, at the moment, the photosensitive sensor senses the external illumination intensity and transmits signals to the second controller, and when the external illumination intensity is large enough, the second controller controls the photosensitive sensor to work; the annular lamp tube set and the infusion pump II are closed; opening the first valve and the second valve, allowing the black ink in the black ink water distribution pipe to fall under the action of gravity, and refluxing the black ink into the black ink storage tank; when the external illumination intensity is enough to the illumination intensity on the front side of the circulating pipe and insufficient to the illumination intensity on the rear side of the circulating pipe, the first lamp tube on the front side of the annular lamp tube group is closed, the first lamp tube on the rear side is opened, the first valve and the second valve on the front side are opened, the second infusion pump is closed, the black ink in the black ink water tube on the front side of the annular black ink shading group falls and flows back to the black ink storage tank, the external light can directly irradiate the front side of the circulating pipe, the first valve on the front side is closed, the first valve on the rear side is opened, the black ink in the black ink storage tank is conveyed to the black ink water tube on the rear side under the action of the second infusion pump, at the moment, the external light cannot directly irradiate the rear side of the circulating pipe, the first lamp tube on the front side of the annular lamp tube group can illuminate the rear side of the circulating pipe, and the first valve on the rear side is closed; the illumination intensity in the circulating pipe is not lower than the standard value all the time, thereby being beneficial to the photosynthetic bacteria to carry out photosynthesis fully and grow rapidly.

The incubator comprises a shell, a heating and insulating layer and a clock, wherein the heating and insulating layer is attached to the inner wall of the shell and used for heating and insulating the interior of the incubator; the clock is positioned on the outer wall of the shell and is used for timing the culture time. During the use, temperature sensor detects the temperature in the incubator and feeds back to controller one, and controller one control and regulation heating heat preservation are to incubator inside heating heat preservation, confirm the time of cultivateing through watching the clock on the casing outer wall.

The stirring module comprises a first motor, a hollow stirring shaft, a shaft sleeve and a first spherical shell, and the first motor is fixed at the upper end of the incubator; the hollow stirring shaft is connected with the first motor; the shaft sleeve is positioned at the lower end of the motor, and a first inner cavity is formed in the shaft sleeve; the hollow stirring shaft is matched with the shaft sleeve; a second spherical shell is arranged at the upper end of the hollow stirring shaft, a plurality of first through holes are formed in the second spherical shell, and the second spherical shell is positioned in the first inner cavity; the second inner cavity is arranged in the first spherical shell, a plurality of second through holes are formed in the first spherical shell, the first inner cavity is communicated with the second inner cavity, a plurality of stirring blades are arranged on the outer wall of the first spherical shell, and a plurality of second lamp tubes are arranged on the outer wall of the first spherical shell. When the device is used, under the control of the first controller, the first motor rotates, the motor drives the hollow stirring shaft to rotate, the hollow stirring shaft drives the first spherical shell to rotate, the stirring blades rotate along with the first spherical shell, meanwhile, nutrient solution is stored in the nutrient box, the first controller controls the nutrient box to fill the nutrient solution into the first inner cavity of the shaft sleeve, the nutrient solution in the shaft sleeve enters the hollow pipe and flows into the second spherical shell, the second spherical shell rotates to throw the nutrient solution into the incubator, the stirring blades rotate to enable the nutrient solution to be uniformly mixed with bacterial solution, meanwhile, the rotation of the stirring blades enables the incubator to be uniformly heated, the local overheating phenomenon cannot occur, the rotation of the stirring blades enables the pH in the incubator to be uniform, the local pH value cannot be too high, and the temperature and the pH in the incubator are suitable for enabling enrichment culture or batch production of photosynthetic bacteria to be smoothly performed; and the second lamp tube is bright, and can illuminate the bacterial liquid to enable the photosynthetic bacteria to carry out photosynthesis.

The environment adjusting module comprises a temperature sensor, a PH detector and a PH adjusting box, the temperature sensor is arranged at one end of the stirring blade and is connected with the controller, and the temperature sensor is used for sensing the temperature in the incubator; the PH detector is arranged at the other end of the stirring blade and is connected with the controller, and the PH detector is used for detecting the PH value in the incubator; the PH adjusting box is positioned beside the incubator; a first pipe is arranged between the PH adjusting box and the shaft sleeve, a third valve is arranged on the first pipe, and the first pipe is communicated with the PH adjusting box and a first inner cavity of the shaft sleeve; and a second pipe is connected between the first inner cavity of the shaft sleeve and the nutrient box, a fourth valve is arranged on the second pipe, and the second pipe is communicated with the first inner cavity of the shaft sleeve and the nutrient box. During the use, temperature sensor and PH detector give controller I with the signal transmission who detects, under the control of controller I and mediation, the temperature of heating heat preservation in to the incubator is regulated and control, the PH regulating fluid is equipped with in the PH regulating box, the PH regulating box carries the PH regulating fluid in the first inner chamber of first pipe to the axle sleeve, the PH regulating fluid gets into the second spherical shell, and flow into first spherical shell from the second spherical shell, the first spherical shell of motor drive rotates, first spherical shell gets rid of the PH regulating fluid and makes the PH value in the PH regulating fluid regulation incubator in getting rid of the incubator, environment in the incubator is adjusted.

The gas exchange module comprises a gas filter, an oxygen removing machine and a vent pipe, wherein the oxygen removing machine is positioned between the gas filter and the incubator, the vent pipe is communicated with the incubator and the oxygen removing machine, and the vent pipe is also communicated with the oxygen removing machine and the gas filter. During the use, under the control of controller one, gas filter can filter the gas that gets into the incubator, makes the gas that gets into in the incubator pure, avoids polluting the incubator, because of photosynthetic bacterium does not like oxygen, carries out the deoxidization through the oxygen removal machine to the gas that gets into in the incubator in order to produce the environment that suitable photosynthetic bacterium grows.

The invention has the beneficial effects that:

1. according to the photosynthetic bacteria culture system, the illumination module and the incubator are combined with each other, so that the illumination module compensates the illumination of the bacterial liquid in the incubator; the photosynthetic bacteria culture is automatically compensated by artificial illumination under the condition of insufficient utilization of natural light, the photosynthesis of the photosynthetic bacteria is improved, and the photosynthetic bacteria culture in a large scale is facilitated.

2. According to the photosynthetic bacteria culture system, the incubator, the controller I, the stirring module, the environment adjusting module, the nutrient box and the gas exchange module are combined with one another, the nutrient box and the environment adjusting module provide proper nutrient solution and a growth environment suitable for growth of photosynthetic bacteria for the incubator, the culture solution in the incubator is uniformly mixed and distributed by the stirring module, nutrition required by the photosynthetic bacteria is balanced, meanwhile, the temperature and PH in the incubator are uniform by the stirring module, local overheating or overhigh local PH value cannot be generated to inhibit culture of the photosynthetic bacteria, and large-scale culture of the photosynthetic bacteria is facilitated.

3. According to the photosynthetic bacteria culture system, the incubator and the gas exchange module are combined with each other, so that oxygen in the incubator is controlled, the growth environment of photosynthetic bacteria is suitable, and enrichment and scale culture of photosynthetic bacteria are facilitated.

Drawings

The invention will be further explained with reference to the drawings.

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a cross-sectional view A-A of FIG. 1;

FIG. 3 is a schematic structural view of a stirring module of the present invention;

in the figure: the device comprises a lighting module 1, a circulating pipe 11, a first infusion pump 111, an annular lamp tube group 12, an annular reflector 13, an annular black ink shading group 14, a black ink main pipe 141, a first valve 142, a second valve 143, a second controller 15, a photosensitive sensor 16, a black ink storage tank 17, a second infusion pump 18, an incubator 2, a bacteria liquid inlet 21, a bacteria liquid outlet 22, a shell 23, a heating and insulating layer 24, a clock 25, a first controller 3, a stirring module 4, a first motor 41, a hollow stirring shaft 42, a shaft sleeve 43, a first spherical shell 44, stirring blades 441, a second lamp tube 45, an environment adjusting module 5, a temperature sensor 51, a pH detector 52, a pH adjusting box 53, a third valve 531, a nutrient box 6, a fourth valve 61, a gas exchange module 7, a gas filter 71, a deaerator 72 and a vent pipe 73.

Detailed Description

In order to make the technical means, the creation characteristics, the achievement purposes and the effects of the invention easy to understand, the invention is further described with the specific embodiments.

As shown in fig. 1 to 3, a photosynthetic bacteria cultivation system comprises a lighting module 1, an incubator 2, a first controller 3, a stirring module 4, an environmental regulation module 5, a nutrition box 6 and a gas exchange module 7, wherein the incubator 2 is used for cultivating photosynthetic bacteria; the illumination module 1 is positioned on one side of the incubator 2, and the illumination module 1 is used for photosynthesis of photosynthetic bacteria; the illumination module 1 is internally provided with a circulating pipe 11, and two ends of the circulating pipe 11 are communicated with the incubator 2; the circulating pipe 11 is provided with a first infusion pump 111, and the first infusion pump 111 is used for conveying liquid in the circulating pipe 11; the stirring module 4 is arranged at the upper end of the incubator 2, and the stirring module 4 is used for uniformly stirring the photosynthetic bacteria and the nutrient solution in the incubator 2; the first controller 3 is positioned at the upper end of the stirring module 4, and the first controller 3 is used for adjusting and controlling the stirring module 4, the gas exchange module 7, the environment adjusting module 5 and the nutrient box 6; the environment adjusting module 5 is arranged on the incubator 2, and the environment adjusting module 5 is used for adjusting the environment in the incubator 2; the nutrient box 6 is positioned beside the stirring module 4, the nutrient box 6 is communicated with the incubator 2, and the nutrient box 6 is used for temporarily storing nutrient solution and conveying the nutrient solution into the incubator 2; the gas exchange module 7 is positioned at the side of the incubator 2, the gas exchange module 7 is communicated with the incubator 2, and the gas exchange module 7 is used for replacing gas in the incubator 2 and removing oxygen in the gas; the upper end of incubator 2 is provided with fungus liquid inlet port 21, and the lower extreme of incubator 2 is provided with fungus liquid outlet 22.

The illumination module 1 comprises an annular lamp tube group 12, an annular reflector 13, an annular black ink shading group 14, a black ink main tube 141, a first valve 142, a second valve 143, a second controller 15, a photosensitive sensor 16, a black ink storage tank 17 and a second infusion pump 18, wherein the annular lamp tube group 12 is a plurality of first lamp tubes which are encircled into a circle; the annular reflector 13 is positioned on the inner side of the annular lamp tube group 12 and used for reflecting light emitted by the annular lamp tube group 12; the circulating pipe 11 is circularly wound on the outer wall of the annular lamp tube group 12; the annular black ink shading group 14 is a plurality of black ink branch pipes which enclose a circle, the annular black ink shading group 14 is sleeved on the outer side of the circulating pipe 11, and the annular black ink shading group 14 is used for shading light rays on the outer side of the circulating pipe 11; the black ink main pipe 141 is positioned at the lower end of the annular black ink shading group 14, and the black ink main pipe 141 is communicated with each black ink branch pipe; the photosensitive sensors 16 are uniformly distributed on the outer wall of the annular black ink shading group 14, and the photosensitive sensors 16 are used for detecting the light intensity outside the annular black ink shading group 14; the second controller 15 is positioned at the lower end of the annular lamp tube group 12, and the second controller 15 transmits signals through the photosensitive sensor 16 to control the opening and closing of the annular lamp tube group 12, the first valve 142, the second valve 143, the second infusion pump 18 and the black ink storage tank 17; the first valve 142 is positioned at the bottom end of the black ink branch pipe, and the first valve 142 is used for controlling the flow direction of the black ink in the black ink branch pipe; the second valve 143 is located at the bottom end of the black ink manifold 141, and the second valve 143 is used for controlling the flow direction of the black ink in the black ink manifold 141; the second infusion pump 18 is positioned at the lower end of the second valve 143, and the second infusion pump 18 is used for conveying the black ink in the black ink storage tank 17 to the black ink branch pipe. When the culture device is used, the first infusion pump 111, the first controller 3, the second controller 15, the first valve 142, the second valve 143, the photosensitive sensor 16, the black ink storage tank 17 and the second infusion pump 18 are started, the first infusion pump 111 extracts bacteria liquid in the culture box 2 and conveys the bacteria liquid to the circulating pipe 11, the bacteria liquid flows in the circulating pipe 11 in a circulating mode all the time, at the moment, the photosensitive sensor 16 senses the external illumination intensity and transmits signals to the second controller 15, and when the external illumination intensity is large enough, the second controller 15 controls the culture device; the annular lamp tube group 12 and the second infusion pump 18 are closed; the first valve 142 and the second valve 143 are opened, the black ink in the black ink water distribution pipe falls under the action of gravity, and the black ink flows back into the black ink storage tank 17; when the intensity of external light is sufficient for the intensity of light on the front side of circulation pipe 11 and insufficient for the intensity of light on the rear side of circulation pipe 11, under the control of the second controller 15, the first lamp tube on the front side of the annular lamp tube group 12 is closed, the first lamp tube on the rear side is opened, the first valve 142 and the second valve 143 on the front side are opened, the second infusion pump 18 is closed, the black ink in the black ink water tube on the front side of the annular black ink shading group 14 falls and returns to the black ink storage tank 17, the external light can be directly emitted to the front side of the circulation pipe 11, the first valve 142 on the front side is closed, the first valve 142 on the rear side is opened, the black ink in the black ink storage tank 17 is transported to the black ink moisture pipe on the rear side by the action of the second infusion pump 18, and at this time, the external light cannot directly irradiate the rear side of the circulating pipe 11, the first lamp tube on the front side of the annular lamp tube group 12 can illuminate the rear side of the circulating pipe 11, and the first valve 142 on the rear side is closed; the illumination intensity in the circulating pipe 11 is always not lower than the standard value, which is beneficial to the photosynthetic bacteria to fully carry out photosynthesis and grow rapidly.

The incubator 2 comprises a shell 23, a heating and insulating layer 24 and a clock 25, wherein the heating and insulating layer 24 is attached to the inner wall of the shell 23, and the heating and insulating layer 24 is used for heating and insulating the interior of the incubator 2; the clock 25 is located on the outer wall of the housing 23, and the clock 25 is used for timing the incubation time. During the use, temperature sensor 51 detects the temperature in incubator 2 and feeds back to controller 3, and controller 3 control and adjust heating heat preservation layer 24 and keep warm to incubator 2 inside heating, confirms the time of cultivateing through looking clock 24 on the casing 23 outer wall.

The stirring module 4 comprises a first motor 41, a hollow stirring shaft 42, a shaft sleeve 43 and a first spherical shell 44, wherein the first motor 41 is fixed at the upper end of the incubator 2; the hollow stirring shaft 42 is connected with a first motor 41; the shaft sleeve 43 is positioned at the lower end of the first motor 41, and a first inner cavity is formed in the shaft sleeve 43; the hollow stirring shaft 42 is matched with the shaft sleeve 43; a second spherical shell is arranged at the upper end of the hollow stirring shaft 42, a plurality of first through holes are formed in the second spherical shell, and the second spherical shell is positioned in the first inner cavity; be provided with the second inner chamber in the first spherical shell 44, all be equipped with a plurality of second through-holes on the first spherical shell 44, first inner chamber and second inner chamber UNICOM all are equipped with a plurality of stirring vane 441 on the outer wall of first spherical shell 44, still all are equipped with a plurality of second fluorescent tubes 45 on the outer wall of first spherical shell 44. When the cultivation box is used, under the control of the first controller 3, the first motor 41 rotates, the first motor 41 drives the hollow stirring shaft 42 to rotate, the hollow stirring shaft 42 drives the first spherical shell 44 to rotate, the stirring blade 441 rotates along with the first spherical shell 44, meanwhile, nutrient solution is stored in the nutrition box 6, the first controller 3 controls the nutrition box 6 to fill nutrient solution into the first inner cavity of the shaft sleeve 43, the nutrient solution in the shaft sleeve 43 enters the hollow pipe and flows into the second spherical shell, the second spherical shell transmits the nutrient solution into the first spherical shell 44, the first spherical shell 44 rotates to throw the nutrient solution into the cultivation box 2, the stirring blade 441 rotates to uniformly mix the nutrient solution with the bacterial solution, meanwhile, the rotation of the stirring blade 441 also enables the cultivation box 2 to be uniformly heated, the local overheating phenomenon cannot occur, the rotation of the stirring blade 441 also enables the pH in the cultivation box 2 to be uniform, and the local pH value cannot be too high, the temperature and PH in the incubator 2 are suitable for the enrichment culture or batch production of the photosynthetic bacteria to be smoothly carried out; the second lamp tube 45 is lighted up, and can illuminate the bacteria liquid to enable the photosynthetic bacteria to carry out photosynthesis.

The environment adjusting module 5 comprises a temperature sensor 51, a PH detector 52 and a PH adjusting box 53, the temperature sensor 51 is arranged at one end of the stirring blade 441, the temperature sensor 51 is connected with the first controller 3, and the temperature sensor 51 is used for sensing the temperature in the incubator 2; the PH detector 52 is arranged at the other end of the stirring blade 441, the PH detector 52 is connected with the first controller 3, and the PH detector 52 is used for detecting the PH value in the incubator 2; the PH adjusting box 53 is positioned beside the incubator 2; a first pipe is arranged between the PH adjusting box 53 and the shaft sleeve 43, a valve III 531 is arranged on the first pipe, and the first pipe is communicated with the PH adjusting box 53 and a first inner cavity of the shaft sleeve 43; a second pipe is connected between the first inner cavity of the shaft sleeve 43 and the nutrient box 6, a valve IV 61 is arranged on the second pipe, and the second pipe is communicated with the first inner cavity of the shaft sleeve 43 and the nutrient box 6. During the use, temperature sensor 51 and PH detector 52 give controller 3 with the signal transmission who detects, under controller 3's control and mediation, the temperature of heating heat preservation layer 24 in to incubator 2 is regulated and control, the PH regulating solution is equipped with in the PH regulating box 53, PH regulating box 53 carries the PH regulating solution in the first inner chamber of axle sleeve 43 through first pipe, the PH regulating solution gets into the second spherical shell, and flow into first spherical shell 44 from the second spherical shell, motor 41 drives first spherical shell 44 and rotates, first spherical shell 44 gets rid of the PH regulating solution and makes the PH value in the PH regulating incubator 2 in incubator 2, environment in incubator 2 adjusts.

The gas exchange module 7 comprises a gas filter 71, an oxygen remover 72 and a ventilation pipe 73, wherein the oxygen remover 72 is positioned between the gas filter 71 and the incubator 2; the ventilation pipe 73 is communicated with the incubator 2 and the oxygen remover 72, and the ventilation pipe 73 is also communicated with the oxygen remover 72 and the gas filter 71. During the use, under the control of controller 3, gas filter 71 can filter the gas that gets into in incubator 2, makes the gas that gets into in incubator 2 pure, avoids polluting incubator 2, because of photosynthetic bacterium does not like oxygen, carries out the deoxidization to the gas that gets into in incubator 2 through oxygen removal machine 72 with the environment that suitable photosynthetic bacterium grows.

The specific working process is as follows:

during operation, under the control of the first controller 3, the gas filter 71 can filter the gas entering the incubator 2, so that the gas entering the incubator 2 is pure, the incubator 2 is prevented from being polluted, and the gas entering the incubator 2 is deoxidized through the deoxidizer 72; under the control of the controller I3, the motor I41 rotates, the motor I41 drives the hollow stirring shaft 42 to rotate, the hollow stirring shaft 42 drives the first spherical shell 44 to rotate, the stirring blade 441 rotates along with the first spherical shell 44, meanwhile, the controller I3 controls the nutrient box 6 to fill nutrient solution into the first inner cavity of the shaft sleeve 43, the nutrient solution in the shaft sleeve 43 enters the hollow pipe and flows into the second spherical shell, the second spherical shell transmits the nutrient solution into the first spherical shell 44, the first spherical shell 44 rotates to throw the nutrient solution into the incubator 2, the stirring blade 441 rotates to uniformly mix the nutrient solution with the bacteria solution, under the control and adjustment of the controller I3, the heating and heat-insulating layer 24 regulates and controls the temperature in the incubator 2, the PH adjusting box 53 is filled with the PH adjusting solution, the PH adjusting box 53 transmits the PH adjusting solution into the first inner cavity of the shaft sleeve 43 through the first pipe, the PH adjusting solution enters the second spherical shell, the pH adjusting liquid flows into the first spherical shell 44 from the second spherical shell, the first motor 41 drives the first spherical shell 44 to rotate, and the first spherical shell 44 throws the pH adjusting liquid into the incubator 2 to enable the pH adjusting liquid to adjust the pH value in the incubator 2, so that the environment in the incubator 2 is adjusted; meanwhile, the stirring blades 441 rotate, so that the incubator 2 is uniformly heated, a local overheating phenomenon cannot be generated, the pH value in the incubator 2 is uniform, a local pH value cannot be too high, and after the second lamp tube 45 is opened, the bacteria liquid can be illuminated, so that photosynthetic bacteria can be subjected to photosynthesis; the enrichment culture or batch production of the photosynthetic bacteria can be smoothly carried out by creating a suitable environment.

After a suitable environment suitable for survival of photosynthetic bacteria is manufactured, the first infusion pump 111, the first controller 3, the second controller 15, the first valve 142, the second valve 143, the photosensitive sensor 16, the black ink storage tank 17 and the second infusion pump 18 are started, the first infusion pump 111 extracts and conveys the bacterial liquid in the incubator 2 to the circulating pipe 11 and enables the bacterial liquid to flow in the circulating pipe 11 in a circulating mode, at the moment, the photosensitive sensor 16 senses the external illumination intensity and transmits signals to the second controller 15, and when the external illumination intensity is large enough through the control of the second controller 15; the annular lamp tube group 12 and the second infusion pump 18 are closed; the first valve 142 and the second valve 143 are opened, the black ink in the black ink water distribution pipe falls under the action of gravity, and the black ink flows back into the black ink storage tank 17; when the intensity of external light is sufficient for the intensity of light on the front side of circulation pipe 11 and insufficient for the intensity of light on the rear side of circulation pipe 11, under the control of the second controller 15, the first lamp tube on the front side of the annular lamp tube group 12 is closed, the first lamp tube on the rear side is opened, the first valve 142 and the second valve 143 on the front side are opened, the second infusion pump 18 is closed, the black ink in the black ink water tube on the front side of the annular black ink shading group 14 falls and returns to the black ink storage tank 17, the external light can be directly emitted to the front side of the circulation pipe 11, the first valve 142 on the front side is closed, the first valve 142 on the rear side is opened, the black ink in the black ink storage tank 17 is transported to the black ink moisture pipe on the rear side by the action of the second infusion pump 18, and at this time, the external light cannot directly irradiate the rear side of the circulating pipe 11, the first lamp tube on the front side of the annular lamp tube group 12 can illuminate the rear side of the circulating pipe 11, and the first valve 142 on the rear side is closed; the illumination intensity in the circulating pipe 11 is always not lower than the standard value, which is beneficial to the photosynthetic bacteria to fully carry out photosynthesis, quickly grow and culture the photosynthetic bacteria in batches.

The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, which are described in the specification and illustrated only to illustrate the principle of the present invention, but that various changes and modifications may be made therein without departing from the spirit and scope of the present invention, which fall within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.

Claims

1. A photosynthetic bacteria culture system characterized in that: the cultivation system comprises a lighting module (1), an incubator (2), a first controller (3), a stirring module (4), an environment adjusting module (5), a nutrient box (6) and a gas exchange module (7), wherein the incubator (2) is used for cultivating photosynthetic bacteria; the illumination module (1) is positioned on one side of the incubator (2), and the illumination module (1) is used for photosynthesis of photosynthetic bacteria; a circulating pipe (11) is arranged in the illumination module (1), and two ends of the circulating pipe (11) are communicated with the incubator (2); the circulating pipe (11) is provided with a first infusion pump (111), and the first infusion pump (111) is used for conveying liquid in the circulating pipe (11); the stirring module (4) is arranged at the upper end of the incubator (2), and the stirring module (4) is used for uniformly stirring the photosynthetic bacteria and the nutrient solution in the incubator (2); the first controller (3) is positioned at the upper end of the stirring module (4), and the first controller (3) is used for adjusting and controlling the stirring module (4), the gas exchange module (7), the environment adjusting module (5) and the nutrient box (6); the environment adjusting module (5) is arranged on the incubator (2), and the environment adjusting module (5) is used for adjusting the environment in the incubator (2); the nutrient box (6) is positioned beside the stirring module (4), the nutrient box (6) is communicated with the incubator (2), and the nutrient box (6) is used for temporarily storing nutrient solution and conveying the nutrient solution into the incubator (2); the gas exchange module (7) is positioned at the side of the incubator (2), the gas exchange module (7) is communicated with the incubator (2), and the gas exchange module (7) is used for replacing gas in the incubator (2) and removing oxygen in the gas; the upper end of incubator (2) is provided with fungus liquid inlet port (21), and the lower extreme of incubator (2) is provided with fungus liquid outlet (22).

2. A photosynthetic bacteria cultivation system according to claim 1 wherein: the illumination module (1) comprises an annular lamp tube group (12), an annular reflector (13), an annular black ink shading group (14), a black ink main pipe (141), a first valve (142), a second valve (143), a second controller (15), a photosensitive sensor (16), a black ink storage tank (17) and a second infusion pump (18), wherein the annular lamp tube group (12) is a plurality of first lamp tubes which are encircled into a circle; the annular reflector (13) is positioned on the inner side of the annular lamp tube group (12) and is used for reflecting light emitted by the annular lamp tube group (12); the circulating pipe (11) is circularly wound on the outer wall of the annular lamp tube group (12); the annular black ink shading group (14) is a plurality of black ink branch pipes which enclose into a circle, the annular black ink shading group (14) is sleeved on the outer side of the circulating pipe (11), and the annular black ink shading group (14) is used for shading light on the outer side of the circulating pipe (11); the black ink main pipe (141) is positioned at the lower end of the annular black ink shading group (14), and the black ink main pipe (141) is communicated with each black ink branch pipe; the photosensitive sensors (16) are uniformly distributed on the outer wall of the annular black ink shading group (14), and the photosensitive sensors (16) are used for detecting the light intensity outside the annular black ink shading group (14); the second controller (15) is positioned at the lower end of the annular lamp tube group (12), and the second controller (15) transmits signals through the photosensitive sensor (16) to control the opening and closing of the annular lamp tube group (12), the first valve (142), the second valve (143), the second infusion pump (18) and the black ink storage tank (17); the first valve (142) is positioned at the bottom end of the black ink branch pipe, and the first valve (142) is used for controlling the flow direction of black ink in the black ink branch pipe; the second valve (143) is positioned at the bottom end of the black ink main pipe (141), and the second valve (143) is used for controlling the flow direction of black ink in the black ink main pipe (141); the second infusion pump (18) is positioned at the lower end of the second valve (143), and the second infusion pump (18) is used for conveying the black ink in the black ink storage tank (17) to the black ink branch pipe.

3. A photosynthetic bacteria cultivation system according to claim 1 wherein: the incubator (2) comprises a shell (23), a heating and insulating layer (24) and a clock (25), the heating and insulating layer (24) is attached to the inner wall of the shell (23), and the heating and insulating layer (24) is used for heating and insulating the interior of the incubator (2); the clock (25) is positioned on the outer wall of the shell (23), and the clock (25) is used for timing the culture time.

4. A photosynthetic bacteria cultivation system according to claim 1 wherein: the stirring module (4) comprises a first motor (41), a hollow stirring shaft (42), a shaft sleeve (43) and a first spherical shell (44), wherein the first motor (41) is fixed at the upper end of the incubator (2); the hollow stirring shaft (42) is connected with a first motor (41); the shaft sleeve (43) is positioned at the lower end of the first motor (41), and a first inner cavity is formed in the shaft sleeve (43); the hollow stirring shaft (42) is matched with the shaft sleeve (43); a second spherical shell is arranged at the upper end of the hollow stirring shaft (42), a plurality of first through holes are formed in the second spherical shell, and the second spherical shell is positioned in the first inner cavity; be provided with the second inner chamber in first spherical shell (44), all be equipped with a plurality of second through-holes on first spherical shell (44), first inner chamber and second inner chamber UNICOM all are equipped with a plurality of stirring vane (441) on the outer wall of first spherical shell (44), still all are equipped with a plurality of second fluorescent tubes (45) on the outer wall of first spherical shell (44).

5. A photosynthetic bacteria cultivation system according to claim 4 wherein: the environment adjusting module (5) comprises a temperature sensor (51), a PH detector (52) and a PH adjusting box (53), the temperature sensor (51) is arranged at one end of the stirring blade (441), the temperature sensor (51) is connected with the controller I (3), and the temperature sensor (51) is used for sensing the temperature in the incubator (2); the PH detector (52) is arranged at the other end of the stirring blade (441), the PH detector (52) is connected with the first controller (3), and the PH detector (52) is used for detecting the PH value in the incubator (2); the PH adjusting box (53) is positioned beside the incubator (2); a first pipe is arranged between the PH adjusting box (53) and the shaft sleeve (43), a third valve (531) is arranged on the first pipe, and the first pipe is communicated with the PH adjusting box (53) and a first inner cavity of the shaft sleeve (43); and a second pipe is connected between the first inner cavity of the shaft sleeve (43) and the nutrient box (6), a valve IV (61) is arranged on the second pipe, and the second pipe is communicated with the first inner cavity of the shaft sleeve (43) and the nutrient box (6).

6. A photosynthetic bacteria cultivation system according to claim 4 wherein: the gas exchange module (7) comprises a gas filter (71), an oxygen remover (72) and a ventilation pipe (73), wherein the oxygen remover (72) is positioned between the gas filter (71) and the incubator (2); the aeration pipe (73) is communicated with the incubator (2) and the oxygen removing machine (72), and the aeration pipe (73) is also communicated with the oxygen removing machine (72) and the gas filter (71).