CN105060463A - Wastewater treatment method adopting O2 produced by photosynthetic organisms to replace traditional aeration as well as application - Google Patents
Wastewater treatment method adopting O2 produced by photosynthetic organisms to replace traditional aeration as well as application Download PDFInfo
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- CN105060463A CN105060463A CN201510492677.6A CN201510492677A CN105060463A CN 105060463 A CN105060463 A CN 105060463A CN 201510492677 A CN201510492677 A CN 201510492677A CN 105060463 A CN105060463 A CN 105060463A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses a wastewater treatment method adopting O2 produced by photosynthetic organisms to replace traditional aeration. Photosynthetic organism cells such as microalgae obtained through pre-culture or obtained from a culture system are added to a transformed oxidative aeration device and reach a certain proportion, and then micro O2 bubbles are produced and secreted under the photosynthesis of the photosynthetic organisms and provided for other microorganisms in a photosynthetic organism-bacterium symbiotic system for growth and metabolism. Compared with the processes for providing large bubbles through mechanical stirring and blowing, the method has the advantages as follows: 1, the method is mild, efficient and energy-saving; 2, the CO2 emission is reduced, CO2 which is to be emitted into the atmosphere originally is recovered and immobilized into biomass under the photosynthesis; 3, the method is environment-friendly, under the condition that existing traditional technologies are basically not changed, the concentration of carbon, nitrogen and phosphorus nutrients in effluent is reduced, and eutrophication of natural water bodies receiving the effluent is reduced.
Description
Technical field
The invention belongs to field of environment protection, also can be used for regenerated biological energy field, or take into account two fields.Be used for the novel process of wastewater treatment in particular to a kind of photosynthetic organism, improve the aeration phase technique of existing wastewater treatment.More particularly, first photosynthetic organism cell is obtained by preculture; Then photosynthetic organism is dropped into transformed aerating apparatus; Under illumination condition, photosynthetic organism produces O
2, and by O
2be secreted in environment; Photosynthetic organism in system and other microorganisms obtain O
2, accelerate metabolism and growth; Microbial process degraded and absorption absorb polluted matter; On the whole, the energy demand of aeration phase is obviously reduced.
Background technology
The photosynthetic organism that the present invention relates to belongs to can carry out photosynthetic, volume microscopic aquatic plant and (or) microorganism, but their photosynthetic efficiency, the speed of growth are higher than traditional terrestrial plant, can Fast back-projection algorithm and accumulation various biomass, comprise the entocytes such as cell, fat, VITAMIN, biomass, through processing, may be used for multiple fields such as industry, agricultural, food.Wherein, micro-algae (as green algas such as chlorellas, and blue-green algae) be the photosynthetic organism that current culture technique is comparatively ripe, application prospect is bright and clear.Traditional technology is by microdisk electrode in aseptic artificial medium, and cost is high, lacks economic competitiveness.Research shows, micro-algae of some kinds can transform carbon element in organic waste water by photosynthesis, and the CO in air or flue gas
2, release O
2.Micro algae growth, metabolism also absorb the nitrogen and phosphorous nutrient adsorbed in waste water, purify liquid waste, and reduce the eutrophication of receiving the natural water body of discharging water.
In conventional waste water second-stage treatment technique, oxidation and aeration is the process of a highly energy-consuming, by air-blowing and mechanical stirring by O
2be transported in system, liquid matter acutely mixes that to break with large bubble be the feature of whole technique.Because O
2solubleness very low, the O carried by air pocket
2the time stopped in a liquid is very short, fully can not be exchanged and enter in liquid, therefore just be difficult to be utilized by microorganism, most of O
2do not utilize and be just emitted in environment, cause energy and O
2waste.And sufficient O
2energy accelerate growth of microorganism and metabolism, ensure the effect of oxidation and aeration technique.Although use the air or O optimized
2system of distribution partly can improve O
2mass transfer effect, but obviously can not reduce energy consumption.
Therefore, a kind of efficient, energy-saving and emission-reduction, economically viable O of exploitation is badly in need of
2location mode.Through research, the photosynthesis of the photosynthetic organisms such as micro-algae is utilized to produce O
2micro-bubble, the mushroom being supplied to symbiosis for metabolism and growth, the O shortened
2transmittance process, improves microorganism-capturing and utilizes O
2efficiency, and photosynthetic organism can be utilized the absorption adsorption of carbon nitrogen and phosphorous nutrient, improve the quality of water outlet.
Summary of the invention
The object of the present invention is to provide the spontaneous O of a kind of photosynthetic organism
2replacing the method for wastewater treatment of traditional aeration, by introducing a certain proportion of photosynthetic organism, producing O by photosynthetic organism photosynthesis
2utilize for photosynthetic organism-bacterium syntaxial system, replace traditional mechanical process oxidation and aeration technique, thus a kind of novel simple efficient, energy-saving and emission-reduction of exploitation, lower-cost wastewater oxidation aeration method.
The technical scheme that the present invention solves the problems of the technologies described above is as follows:
The spontaneous O of a kind of photosynthetic organism
2replace the method for wastewater treatment of traditional aeration, it is photosynthetic organism (as the micro-algae) cell using preculture to obtain or gather in the crops from culture system, join in improved oxidation and aeration device, make it to reach certain ratio, then produced by the photosynthesis of photosynthetic organism and secrete small O
2bubble, gives other microorganisms in photosynthetic organism-bacterium syntaxial system for metabolism and growth.
Above-mentioned improved oxidation and aeration device is by store battery 1, photovoltaic panel 2, computer automatic control system 3, first light source 4-1, secondary light source 4-2, unpasteurized waste water, photosynthetic organism and bacterium water inlet pipe 5; Water outlet pipe 6 after process, pH electrode 7, OD electrode 8, dissolved oxygen electrode 9, phosphate radical electrode 10, ammonia nitrogen/nitrate electrode 11, inorganic carbon electrode 12, light intensity electrode 13, aeration tank (including waste water, photosynthetic organism and bacterium) 14, microbubble generator 15, under meter 16, valve 17, reducing valve 18, CO2/ mixing air/flue gas or other mixing gas cylinders 19 form.
Waste water, photosynthetic organism and bacterium is had in described aeration tank 14.Unpasteurized wastewater inlet pipe 5 inserts in aeration tank 1 from aeration tank 14.Water outlet pipe 6 after described process inserts bottom aeration tank 14 from aeration tank 14.
Described pH electrode 7, OD electrode 8, dissolved oxygen electrode 9, phosphate radical electrode 10, ammonia nitrogen/nitrate electrode 11, inorganic carbon electrode 12 and light intensity electrode 13 parallel connection are placed in aeration tank 14.
Described first light source 4-1 is arranged on top, aeration tank 14.Described secondary light source 4-2 is arranged on side, aeration tank 14.Described microbubble generator 15 is arranged on the end, aeration tank 14.
The pH electrode 7 of described parallel connection, OD electrode 8, dissolved oxygen electrode 9, phosphate radical electrode 10, ammonia nitrogen/nitrate electrode 11, inorganic carbon electrode 12 are connected with described computer automatic control system 3 with light intensity electrode 13.
Described store battery 1 is connected with photovoltaic panel 2, and store battery 1 is connected with the first light source 4-1, secondary light source 4-2 respectively.
Described CO2/ mixing air/flue gas or other mixing gas cylinders 19 export and reducing valve 18, valve 17, under meter 16 are housed successively, and under meter 16 is connected with microbubble generator 15.
Concrete operation step is as follows:
1. the first light source 4-1 is arranged on top, aeration tank 14, secondary light source 4-2 is arranged on side, aeration tank 14.Adjustment distance, makes intensity of illumination reach 1500Lux ~ 5000Lux, forms illumination aeration tank.
2. pending unpasteurized waste water, photosynthetic organism and bacterium are injected in illumination aeration tank 14 by unpasteurized waste water, photosynthetic organism and bacterium water inlet pipe 5, then added the photosynthetic organism obtained by preculture or recycling step by unpasteurized waste water, photosynthetic organism and bacterium water inlet pipe 5, photosynthetic organism is mixed with the microorganism in water.Described photosynthetic organism such as Chlorella, Scenedesmus, blue-green algae are one or more of seawater or fresh water algae or photosynthetic bacterium; Microorganism in described water is non-photosynthetic organism, hereinafter referred to as bacterium.
3. logical store battery 1 is connected with photovoltaic panel 2, and store battery 1 is connected with the first light source 4-1, secondary light source 4-2 respectively, and under illumination condition, the photosynthetic organism in aeration tank 14 utilizes the carbon in water, carries out photosynthesis release O
2.
4. the O of photosynthetic organism release
2absorbed by bacterium, for metabolism and growth, under aerobic conditions, bacterium can organism more fully in degradation water, and discharges carbon.
5. carbon that organic matter degradation discharges in waste water, by photosynthetic organism further by the more O of photosynthesis release
2, increase the dissolving O of aeration tank
2, promote the growth of photosynthetic organism and bacterium, metabolism, propagation, constantly increase the various biomass in aeration tank.The photosynthetic organism be on the increase and bacterium, through constantly mixing and interaction, finally form the mixture with the various observable form of small-particle.
6. the gas of CO2/ mixing air/flue gas or other mixing gas cylinders 19 is passed through reducing valve 18, valve 17, under meter 16, under meter 16 is connected with microbubble generator 15, and microbubble generator enters in the aeration tank containing photosynthetic organism-bacterium with the form of microvesicle.On the one hand, under microvesicle state, CO
2can be dissolved in water more fully, supplement photosynthetic raw material CO
2; On the other hand, in microvesicle uphill process, drive liquid rising, promoted the mixing of photosynthetic organism-bacterium, avoided the formation of too much precipitation and reduce the photosynthetic organism-bacterium particle participating in water treatment.
7. be connected with described computer automatic control system 3 by pH electrode 7, OD electrode 8, dissolved oxygen electrode 9, phosphate radical electrode 10, ammonia nitrogen/nitrate electrode 11, inorganic carbon electrode 12 and light intensity electrode 13, the physical and chemical index such as total nitrogen, total phosphorus of the water after check processing, to determine that illumination aeration tank reaches the effect of traditional mechanical stirring aeration, collecting by filtration photosynthetic organism-bacterium mixture, water after process enters other operations by the water outlet pipe 6 after process, or qualified discharge.
Advantage of the present invention:
1. gentle efficient.Bring the process of air pocket to compare with mechanical stirring and air-blowing, photosynthetic organism produces O
2whole process as mild as a dove, there is no the injury that mechanical shearing produces cell, and a newborn O
2volume is little, purity is high, and the efficiency utilized nearby by microorganism is higher.
2. energy-conservation.The high-power machinery in traditional technology is no longer needed to stir and air-blowing; The energy consumption of lower part for illumination can also be economized further at warmer climate and region with abundant light supply;
3. reduce discharging CO
2.With containing CO
2air and gas mixture, or industrial gaseous waste produces the microvesicle of liquid matter circulation mixing, not only saves O
2, and will originally need the CO be discharged in air
2reclaim and be fixed into biomass through photosynthesis.
4. environmental friendliness.Under the condition substantially not changing existing traditional technology, reduce the carbon nitrogen and phosphorus salt concn of discharging in water, reduce the eutrophication of receiving the natural water body of discharging water.
Accompanying drawing explanation
Fig. 1 the present invention improved oxidation and aeration apparatus structure schematic diagram.
In figure, store battery 1, photovoltaic panel 2, computer automatic control system 3, first light source 4-1, secondary light source 4-2, unpasteurized wastewater inlet pipe 5; Water outlet pipe 6 after process, pH electrode 7, OD electrode 8, dissolved oxygen electrode 9, phosphate radical electrode 10, ammonia nitrogen/nitrate electrode 11, inorganic carbon electrode 12, light intensity electrode 13, aeration tank (including waste water, photosynthetic organism, other microorganisms) 14, microbubble generator 15, under meter 16, valve 17, reducing valve 18, CO2/ mixing air/flue gas or other gas mixtures 19.
Fig. 2 embodiment of the present invention 1 processes the total nitrogen total phosphorus variation diagram of wastewater from starch industry (former water, non-sterilizing).
Fig. 3 embodiment of the present invention 2 processes the total nitrogen total phosphorus variation diagram in rotten starch Heisui River (non-sterilizing).
Fig. 4 embodiment of the present invention 3 processes wastewater from starch industry total nitrogen total phosphorus variation diagram.
Fig. 5 embodiment of the present invention 4 processes wastewater from starch industry total nitrogen total phosphorus variation diagram.
Embodiment
The spontaneous O of a kind of photosynthetic organism of the present invention
2replace in the method for wastewater treatment of traditional aeration, if do not have illumination (inner from the system away from daylight or source of artificial light, or during dark cycle such as night), photosynthetic organism also participates in the biochemical action process in aeration tank, carries out the organic substance in heterotrophic growth sustaining degradation conversion of waste water together with bacterium.Under light illumination, photosynthetic organism carries out photosynthesis generation O
2, the O discharged
2caught nearby for metabolism and growth by photosynthetic organism-bacterium syntaxial system; The bubble phase ratio produced with traditional mechanical stirring and air-blowing, newly-generated O
2volume is little, purity is high, is more easily utilized by microorganism, result O
2utilising efficiency improves, the air no longer needing traditional technology a large amount of like that or O
2.In addition, photosynthetic organism, the particularly participation of micro-algae, compensate for the deficiency that active sludge microorganism in traditional technology is weak to carbon nitrogen phosphorus, Metal uptake adsorptive power, by gathering in the crops this photosynthetic organism such as algae slightly, the carbon nitrogen phosphorus in waste water and metal nutritive salt can be taken away further, the quality of discharge water is improved.
The improved oxidation and aeration apparatus structure of the present invention as shown in Figure 1, by store battery 1, photovoltaic panel 2, computer automatic control system 3, first light source 4-1, secondary light source 4-2, unpasteurized waste water, photosynthetic organism and bacterium water inlet pipe 5; Water outlet pipe 6 after process, pH electrode 7, OD electrode 8, dissolved oxygen electrode 9, phosphate radical electrode 10, ammonia nitrogen/nitrate electrode 11, inorganic carbon electrode 12, light intensity electrode 13, aeration tank (including waste water, photosynthetic organism and bacterium) 14, microbubble generator 15, under meter 16, valve 17, reducing valve 18, CO2/ mixing air/flue gas or other mixing gas cylinders 19 form.
Waste water, photosynthetic organism and bacterium is had in described aeration tank 14.Unpasteurized wastewater inlet pipe 5 inserts in aeration tank 1 from aeration tank 14.Water outlet pipe 6 after described process inserts bottom aeration tank 14 from aeration tank 14.
Described pH electrode 7, OD electrode 8, dissolved oxygen electrode 9, phosphate radical electrode 10, ammonia nitrogen/nitrate electrode 11, inorganic carbon electrode 12 and light intensity electrode 13 parallel connection are placed in aeration tank 14.
Described first light source 4-1 is arranged on top, aeration tank 14.Described secondary light source 4-2 is arranged on side, aeration tank 14.Described microbubble generator 15 is arranged on the end, aeration tank 14.
The pH electrode 7 of described parallel connection, OD electrode 8, dissolved oxygen electrode 9, phosphate radical electrode 10, ammonia nitrogen/nitrate electrode 11, inorganic carbon electrode 12 are connected with described computer automatic control system 3 with light intensity electrode 13.
Described store battery 1 is connected with photovoltaic panel 2, and store battery 1 is connected with the first light source 4-1, secondary light source 4-2 respectively.
Described CO2/ mixing air/flue gas or other mixing gas cylinders 19 export and reducing valve 18, valve 17, under meter 16 are housed successively, and under meter 16 is connected with microbubble generator 15.
Concrete operation step is as follows:
1. the first light source 4-1 is arranged on top, aeration tank 14, secondary light source 4-2 is arranged on side, aeration tank 14.Adjustment distance, makes intensity of illumination reach 1500Lux ~ 5000Lux, forms illumination aeration tank.
2. pending unpasteurized waste water, photosynthetic organism and bacterium are injected in illumination aeration tank 14 by unpasteurized waste water, photosynthetic organism and bacterium water inlet pipe 5, then added the photosynthetic organism obtained by preculture or recycling step by unpasteurized waste water, photosynthetic organism and bacterium water inlet pipe 5, photosynthetic organism is mixed with the microorganism in water.Described photosynthetic organism such as Chlorella, Scenedesmus, blue-green algae are one or more of seawater or fresh water algae or photosynthetic bacterium; Microorganism in described water is non-photosynthetic organism, hereinafter referred to as bacterium.
3. logical store battery 1 is connected with photovoltaic panel 2, and store battery 1 is connected with the first light source 4-1, secondary light source 4-2 respectively, and under illumination condition, the photosynthetic organism in aeration tank 14 utilizes the carbon in water, carries out photosynthesis release O
2.
4. the O of photosynthetic organism release
2absorbed by bacterium, for metabolism and growth, under aerobic conditions, bacterium can organism more fully in degradation water, and discharges carbon.
5. carbon that organic matter degradation discharges in waste water, by photosynthetic organism further by the more O of photosynthesis release
2, increase the dissolving O of aeration tank
2, promote the growth of photosynthetic organism and bacterium, metabolism, propagation, constantly increase the various biomass in aeration tank.The photosynthetic organism be on the increase and bacterium, through constantly mixing and interaction, finally form the mixture with the various observable form of small-particle.
6. the gas of CO2/ mixing air/flue gas or other mixing gas cylinders 19 is passed through reducing valve 18, valve 17, under meter 16, under meter 16 is connected with microbubble generator 15, and microbubble generator enters in the aeration tank containing photosynthetic organism-bacterium with the form of microvesicle.On the one hand, under microvesicle state, CO
2can be dissolved in water more fully, supplement photosynthetic raw material CO
2; On the other hand, in microvesicle uphill process, drive liquid rising, promoted the mixing of photosynthetic organism-bacterium, avoided the formation of too much precipitation and reduce the photosynthetic organism-bacterium particle participating in water treatment.
7. be connected with described computer automatic control system 3 by pH electrode 7, OD electrode 8, dissolved oxygen electrode 9, phosphate radical electrode 10, ammonia nitrogen/nitrate electrode 11, inorganic carbon electrode 12 and light intensity electrode 13, the physical and chemical index such as total nitrogen, total phosphorus of the water after check processing, to determine that illumination aeration tank reaches the effect of traditional mechanical stirring aeration, collecting by filtration photosynthetic organism-bacterium mixture, water after process enters other operations by the water outlet pipe 6 after process, or qualified discharge.
Below in conjunction with the embodiment applied, the invention will be further described.
Embodiment 1
The spontaneous O of photosynthetic organism
2replace the method for wastewater treatment of traditional aeration as follows for the original sub-block step of fresh wastewater from starch industry:
Chlorella (Cholorellavulgaris) is local screening.Aspergillus niger (Aspergillusniger) is for mimic photosynthesis biology-bacterium syntaxial system.
Use the fermentor tank (blade diameter length ratio=3) of 3.6L glass material (transparent) as aeration tank (container), 2 × 5wLED (cold light source, white light) is installed for illumination at tank wall, daytime uses natural lighting, uses source of artificial light light filling night;
The former water (non-sterilizing) loading 2.5 liters of fresh wastewater from starch industry is substratum; Access 100mL preculture chlorella (1 × 10
7and 1mL aspergillus niger spore suspension (1 × 10 cells/mL)
8cells/mL);
Under room temperature, quiescent culture chlorella, every day passes into air 15min (3L/min) by microbubble device (being made up of the fermentor tank air-distributor of 12W fish jar inflator, conduit and wrapped wire net), promotes that liquid matter mixes and supplements the CO of photosynthesis needs
2.
Illumination and bubbling use 100Ah photovoltaic power generation apparatus to provide electric energy.
Experimental result, under static condition, photosynthetic organism (chlorella) trends towards being attached on the wall of container close to side light source, and biomass reaches 0.3g/L (dry weight), the O that photosynthesis produces
2facilitate Aspergillus Niger Growth, the mycelia parcel chlorella of prolongation, forms the irregular alga-bacterium symbiosis body of form; Waste water becomes green from yellow, after be progressively wrapped into symbiote along with frustule, waste water becomes clarification, of light color, nitrogen and phosphorus content all just significantly reduces at first day, then maintain lower level, until some nitrogen phosphoric of degraded release occur part microorganism, result as shown in Figure 2.
Embodiment 2
The spontaneous O of photosynthetic organism
2the method of wastewater treatment of traditional aeration is replaced to turn black for corruption and to contain the treatment step of the wastewater from starch industry of more microorganism as follows:
Chlorella (Cholorellavulgaris), aspergillus niger (Aspergillusniger) are local screening, for mimic photosynthesis biology-bacterium syntaxial system.
Ambient temperatare is put 2 weeks, corrupt turn black and the wastewater from starch industry (former water, non-sterilizing) containing more microorganism as substratum.
The transparent drum (blade diameter length ratio=1.5) of 15.0L polypropylene material is used to test as aeration tank (container), at tank body top 15cm height, 2 × 18wLED (mixture colors is installed, white light, key lighting), it is (red: indigo plant=1:1 that 2 × 5wLED is installed in side, additional illumination), 24h illumination;
Load 12.0 liters of wastewater medium, the micro-algae (1 × 10 of access 1000mL preculture
7cells/mL), 10mL aspergillus niger spore suspension (1 × 10
8cells/mL);
Under room temperature, quiescent culture chlorella, every day passes into air 2 times by microbubble device (the fish jar Bubbled stone be made up of 12W fish jar inflator, conduit, agglomerated material and external parcel woven wire form), each 15min (3L/min), promotes that liquid matter mixes and supplements the CO of photosynthesis needs
2.
Illumination and bubbling electric energy are from 100Ah photovoltaic power generation apparatus.
Experimental result: Initial stage of culture, by the effect of aspergillus niger, the atrament of degrading in corrupt waste water, liquid becomes clarification, and algae starts photosynthesis, and system progressively becomes green; Then through the acting in conjunction of algae-bacterium, total nitrogen continuous decrease was by the 4th day, and total phosphorus drops to lower level at first day, after progressively maintain low-level.Until there are some nitrogen phosphoric of degraded release in part microorganism.Alga-bacterium symbiosis body is also irregular in form.Chlorella (photosynthetic organism) reaches 4.1g/L (weight in wet base) and produces O
2to the growth of aspergillus niger and the clarification of system (waste water), there is promoter action; Finally, a large amount of chlorella cells is gathered in the container side wall of red and blue LED supplementary light, and waste water becomes faint yellow clarification.Remove the result of nitrogen phosphorus as Fig. 3.
Embodiment 3
The spontaneous O of photosynthetic organism
2replace the method for wastewater treatment of traditional aeration as follows for the treatment step of the former water boil 10min killing microorganisms nourishing body of fresh wastewater from starch industry:
Chlorella (Chlorellasorokiniana), aspergillus niger (Aspergillusniger) are local screening.
As substratum after the former water boil 10min killing microorganisms nourishing body of fresh wastewater from starch industry.
The stainless cylinder of steel of 10.0L (blade diameter length ratio about=2.5) is used to test as aeration tank (container), 1 × 11w electricity-saving lamp (6500K is installed in tank body upper opening position, warm colour), use in lampshade optically focused directive tank, electricity-saving lamp end-to-end distance is from tank body height 10cm.
Load 8.0 liters of wastewater medium, the micro-algae (1 × 10 of access 500mL preculture
7cells/mL), 10mL aspergillus niger spore suspension (1 × 10
8cells/mL);
Under room temperature, periodicity of illumination (20h illumination: 4h is dark, automatically controls with timer), quiescent culture chlorella, every day helps mixing 2 times, each 10min with miniature immersible pump (5W fish jar is used), promotes that liquid matter mixes and promotes CO in air
2enter, with supplementary photosynthesis raw material.
The electric energy of illumination and mixing is from 100Ah photovoltaic power generation apparatus.
Experimental result: Initial stage of culture, the material in aspergillus niger degrading waste water, system is light yellow, has tart flavour; Along with cultivation process, system deepens to become black gradually, there is bilgy odour, and there is particulate state microbial consortia not of uniform size, finally, liquid becomes greyish-green clarification, frustule concentration reaches 3.4g/L (weight in wet base), mainly in suspension, precipitate on a small quantity, but on the wall non-cohesive.Actinomycetes (Actinomycetes) are found in symbiote.Although the time of system clarification is longer, similar with the result of not boiling killing microorganisms nourishing body to nitrogen phosphorus ligands effect, as shown in Figure 4.
Embodiment 4
The spontaneous O of photosynthetic organism
2replace the method for wastewater treatment of traditional aeration as follows for the treatment step of the former water boil 10min killing microorganisms nourishing body of fresh wastewater from starch industry:
With the natural algae colony of TAP substratum from local water body enrichment culture, alga cells (is adjusted to 1 × 10 by the pre-culture as photosynthetic organism
7cells/mL), the aspergillus niger (Aspergillusniger) of local screening.
As substratum after the former water boil 10min killing microorganisms nourishing body of fresh wastewater from starch industry.
Use 3.0L triangular flask to test as aeration tank (container), load 2.0 liters of wastewater medium, the micro-algae mixed solution of access 50mL preculture, 1mL aspergillus niger spore suspension (1 × 10
8cells/mL); Under being placed in 3 × 18w plant growth lamp (white light).
Under room temperature, 24h illumination quiescent culture chlorella, shake every day helps liquid matter to mix 2 times, each 0.5min.
Illumination electric energy is from civil power.
Experimental result: system is very fast is beige from light yellow, then is transformed into a bit green khaki color of band; Have fine granularity microbial consortia to be formed, finally, system shoal brown clarification, on the wall formed hold flaky semi-transparent film, algae formed green group be distributed in brokenly on film.Preliminary identification of morphology, system, based on Chlorella (Chlorella) and blue-green algae (Cyanophyceae), has a small amount of Scenedesmus (Scenedesmus) and other microorganisms.The time shorten clarified after system access mixing algae, forms special membrane structure, shows, use the mixed system of photosynthetic organism (micro-algae) in the present invention, better effects if, be more conducive to the oxidation and aeration process of wastewater treatment.System does not have significant difference on the nitrogen phosphorus ligands time, as shown in Figure 5.
Claims (1)
1. the spontaneous O of photosynthetic organism
2replace the method for wastewater treatment of traditional aeration, it is characterized in that, be use preculture to obtain or the photosynthetic organism cell gathered in the crops from culture system as micro-algae, join in improved oxidation and aeration device, make it to reach certain ratio, then produced by the photosynthesis of photosynthetic organism and secrete small O
2bubble, gives other microorganisms in photosynthetic organism-bacterium syntaxial system for metabolism and growth;
Described improved oxidation and aeration device is by store battery (1), photovoltaic panel (2), computer automatic control system (3), the first light source (4-1), secondary light source (4-2), unpasteurized waste water, photosynthetic organism and bacterium water inlet pipe (5); Water outlet pipe (6) after process, pH electrode (7), OD electrode (8), dissolved oxygen electrode (9), phosphate radical electrode (10), ammonia nitrogen/nitrate electrode (11), inorganic carbon electrode (12), light intensity electrode (13), aeration tank (including waste water, photosynthetic organism and bacterium) (14), microbubble generator (15), under meter (16), valve (17), reducing valve (18), CO2/ mixing air/flue gas or other mixing gas cylinder (19) compositions;
There are waste water, photosynthetic organism and bacterium in described aeration tank in (14), and unpasteurized wastewater inlet pipe (5) inserts in aeration tank (1) from aeration tank (14); Water outlet pipe (6) after described process inserts bottom, aeration tank (14) from aeration tank 14;
Described pH electrode (7), OD electrode (8), dissolved oxygen electrode (9), phosphate radical electrode (10), ammonia nitrogen/nitrate electrode (11), inorganic carbon electrode (12) and light intensity electrode (13) parallel connection are placed in aeration tank (14);
Described first light source (4-1) is arranged on aeration tank (14) top; Described secondary light source (4-2) is arranged on aeration tank (14) side; Described microbubble generator (15) is arranged on (14) end, aeration tank;
The pH electrode (7) of described parallel connection, OD electrode (8), dissolved oxygen electrode (9), phosphate radical electrode (10), ammonia nitrogen/nitrate electrode (11), inorganic carbon electrode (12) are connected with described computer automatic control system (3) with light intensity electrode (13);
Described store battery (1) is connected with photovoltaic panel (2), and store battery (1) is connected with the first light source (4-1), secondary light source (4-2) respectively;
Reducing valve (18), valve (17), under meter (16) are equipped with in described CO2/ mixing air/flue gas or other mixing gas cylinder (19) outlets successively, and under meter (16) is connected with microbubble generator (15);
Concrete operation step is as follows:
1) the first light source (4-1) is arranged on aeration tank (14) top, secondary light source (4-2) is arranged on aeration tank (14) side, adjustment distance, makes intensity of illumination reach 1500Lux ~ 5000Lux, forms illumination aeration tank;
2) pending unpasteurized waste water is injected in illumination aeration tank (14) by unpasteurized waste water, photosynthetic organism and bacterium water inlet pipe (5), then added the photosynthetic organism obtained by preculture or recycling step by unpasteurized waste water, photosynthetic organism and bacterium water inlet pipe (5), photosynthetic organism is mixed with the microorganism in water; Described photosynthetic organism such as Chlorella, Scenedesmus, blue-green algae are one or more of seawater or fresh water algae or photosynthetic bacterium; Microorganism in described water is non-photosynthetic organism, hereinafter referred to as bacterium;
3) store battery (1) is connected with photovoltaic panel (2), store battery (1) is connected with the first light source (4-1), secondary light source (4-2) respectively, under illumination condition, photosynthetic organism in aeration tank (14) utilizes the carbon in water, carries out photosynthesis release O
2;
4) O of photosynthetic organism release
2absorbed by bacterium, for metabolism and growth, under aerobic conditions, bacterium can organism more fully in degradation water, and discharges carbon;
5) carbon that organic matter degradation discharges in waste water, by photosynthetic organism further by the more O of photosynthesis release
2, increase the dissolving O of aeration tank
2, promote the growth of photosynthetic organism and bacterium, metabolism, propagation, constantly increase the various biomass in aeration tank.The photosynthetic organism be on the increase and bacterium, through constantly mixing and interaction, finally form the mixture with the various observable form of small-particle;
6) gas of CO2/ mixing air/flue gas or other mixing gas cylinder (19) is passed through reducing valve (18), valve (17), under meter (16), under meter (16) is connected with microbubble generator (15), microbubble generator enters in the aeration tank containing photosynthetic organism-bacterium with the form of microvesicle, on the one hand, under microvesicle state, CO
2can be dissolved in water more fully, supplement photosynthetic raw material CO
2; On the other hand, in microvesicle uphill process, drive liquid rising, promoted the mixing of photosynthetic organism-bacterium, avoided the formation of too much precipitation and reduce the photosynthetic organism-bacterium particle participating in water treatment;
7) be connected with described computer automatic control system (3) by pH electrode (7), OD electrode (8), dissolved oxygen electrode (9), phosphate radical electrode (10), ammonia nitrogen/nitrate electrode (11), inorganic carbon electrode (12) and light intensity electrode (13), the physical and chemical index such as total nitrogen, total phosphorus of the water after check processing, to determine that illumination aeration tank reaches the effect of traditional mechanical stirring aeration, collecting by filtration photosynthetic organism-bacterium mixture, water after process enters other operations by the water outlet pipe (6) after process, or qualified discharge.
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105621802A (en) * | 2016-01-19 | 2016-06-01 | 华夏亿科信息技术（北京）有限公司 | Photosynthetic organism-mediated wastewater comprehensive ecological treatment system and method |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003265936A (en) * | 2002-03-13 | 2003-09-24 | Ishikawajima Harima Heavy Ind Co Ltd | Water treatment apparatus |
| CN101575567A (en) * | 2009-06-22 | 2009-11-11 | 北京科技大学 | Method for culturing microalgae by illumination way and reactor thereof |
| CN202530075U (en) * | 2012-05-18 | 2012-11-14 | 哈尔滨商业大学 | Reactor for culturing photosynthetic microorganisms |
| CN104743677A (en) * | 2015-03-06 | 2015-07-01 | 浙江工业大学 | Method for treating wastewater by utilizing immobilized photosynthetic bacterium coupled film reaction system |
-
2015
- 2015-08-12 CN CN201510492677.6A patent/CN105060463A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2003265936A (en) * | 2002-03-13 | 2003-09-24 | Ishikawajima Harima Heavy Ind Co Ltd | Water treatment apparatus |
| CN101575567A (en) * | 2009-06-22 | 2009-11-11 | 北京科技大学 | Method for culturing microalgae by illumination way and reactor thereof |
| CN202530075U (en) * | 2012-05-18 | 2012-11-14 | 哈尔滨商业大学 | Reactor for culturing photosynthetic microorganisms |
| CN104743677A (en) * | 2015-03-06 | 2015-07-01 | 浙江工业大学 | Method for treating wastewater by utilizing immobilized photosynthetic bacterium coupled film reaction system |
Cited By (13)
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| CN106145389A (en) * | 2016-08-25 | 2016-11-23 | 山东科技大学 | The sewage-treatment plant of cytoalgae bacillus cereus co-mixing system and using method thereof |
| CN107047429A (en) * | 2016-12-30 | 2017-08-18 | 黎清华 | VHD industrializes pond ecological breeding system and its regulation and control method |
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| CN107935186A (en) * | 2017-11-24 | 2018-04-20 | 吉林建筑大学 | A kind of bacteria and algae membrane reactor using solar-heating |
| CN108179100A (en) * | 2018-03-26 | 2018-06-19 | 尹康康 | A kind of photosynthetic bacteria screening and culturing method |
| CN108410723A (en) * | 2018-03-26 | 2018-08-17 | 尹康康 | A kind of photosynthetic bacteria culture systems |
| CN108179100B (en) * | 2018-03-26 | 2021-06-08 | 山东百沃生物科技有限公司 | Photosynthetic bacteria screening and culturing method |
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