CN111248139B - Culture device and method for high-density rotifers - Google Patents
Culture device and method for high-density rotifers Download PDFInfo
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- CN111248139B CN111248139B CN202010229759.2A CN202010229759A CN111248139B CN 111248139 B CN111248139 B CN 111248139B CN 202010229759 A CN202010229759 A CN 202010229759A CN 111248139 B CN111248139 B CN 111248139B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/20—Culture of aquatic animals of zooplankton, e.g. water fleas or Rotatoria
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/045—Filters for aquaria
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/32—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of substances in solution
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Abstract
The invention discloses a high-density rotifer culture device and a high-density rotifer culture method, wherein the rotifer culture device comprises a culture tank, a microalgae culture barrel and a central control system, and the high-density rotifer culture method comprises the following steps: pretreatment of a wheel worm culture device; culturing microalgae; culturing rotifers; collecting rotifers; circularly culturing; the culture device is characterized in that a central control system controls microalgae culture barrels and continuously supplies carbon dioxide to accelerate growth of microalgae, the microalgae culture barrels are controlled to convey high-density microalgae serving as bait to a culture tank, pure oxygen is filled to supply rotifer to grow, and after rotifers grow to a certain density, the central control system discharges sewage from the culture tank and collects rotifers, and the rotifer culture solution is recycled.
Description
Technical Field
The invention relates to the field of biological culture, in particular to a method and a device for culturing high-density rotifers.
Background
Rotifers are smiling multicellular organisms which are not only rich in three basic nutrients, namely protein, fat and carbohydrate, but also contain various amino acids, vitamins, highly unsaturated fatty acids and other various bioactive substances. In the field of aquatic organism domestication, the bait is often used as living bait for young seedlings, and has the advantages of excellent feeding effect, rapid self-propagation, strong vitality and proper size, thereby providing the best bait for the domestication of fish and shrimp aquatic organisms and being widely applied to aquaculture.
In recent years, for the protection of ecological aquatic resources, the protection of water-return resources and the implementation of continuous plan are carried out in seedling raising and cultivation, and the growing importance is attached to the cultivation of aquatic seedlings, so that the development of various better and more economic baits is promoted, at present, the most used and highest cost performance rotifers are mainly adopted in the main cultivation mode in China by adopting open outdoor large ponds and organic matter fermentation fat water, and the cultivation has season limitation, weather limitation, and is difficult to control the quantity of the species and the influence of the outside world is large.
Disclosure of Invention
Aiming at the defects, the invention discloses a high-density rotifer culture device, a central control system is used for adjusting the culture environment, and the rotifer is propagated in a room with high density, and also discloses a culture method based on the device, and a culture means for the rotifer and rotifer baits and a method for rapidly and stably culturing the high-density rotifers by using the device are introduced.
In order to achieve the above purpose, the present invention provides the following technical solutions: the culture device for the high-density rotifers is characterized by comprising a central control system, and a microalgae culture barrel and a culture tank which are controlled by the central control system;
the central control system comprises a plurality of sensors arranged in the microalgae culture tank and the culture tank, an oxygenerator for producing pure oxygen and a carbon dioxide generator for producing carbon dioxide, wherein the oxygenerator and the carbon dioxide generator are respectively connected with nano gas stone pipes arranged in the culture tank and the microalgae culture tank;
the microalgae cultivation barrel comprises a constant temperature device and a salinity regulator which are connected with a central control system, an outlet pipeline with a flowmeter is arranged at the bottom of the microalgae cultivation barrel, an air lifting mechanism is further arranged, the air lifting mechanism is provided with a gas distributor which extends into the bottom of the microalgae cultivation barrel, and the outlet pipeline is further connected with a water inlet A of a cultivation groove;
the cultivation tank comprises a constant temperature device, a salinity regulator and a liquid level regulating pipe which are connected with a central control system, a collection assembly of wheel worms is arranged in the cultivation tank, the cultivation tank further comprises a water outlet arranged at the bottom and a micro-filter, the water outlet is opposite to the micro-filter, the micro-filter is tightly attached to the cultivation tank, the micro-filter comprises a drainage pipeline, and a disinfection sedimentation barrel is connected through the drainage pipeline.
Further, the central control system further comprises a plurality of water pumps.
Further, a rotary mesh for filtering and collecting is arranged in the wheel worm collecting assembly, and the mesh aperture is 80-100 microns.
The invention also comprises a culture method of the high-density rotifer for the device, which comprises the following steps:
step one: pretreatment of a wheel worm culture device: cleaning the culture device with clear water, exposing the culture device to the sun for 2d under the outdoor sun condition, taking back to the heat preservation greenhouse, standing for 1d and the like, and cooling;
step two: microalgae cultivation: introducing microalgae culture solution into a microalgae culture barrel through a water inlet, inoculating the microalgae which is actively cultured and selected in advance and has no pollution, setting parameters of indoor microalgae culture through a central control system, namely starting a carbon dioxide generator for 0.5h every 3h to provide basic conditions for microalgae photosynthesis, starting an air lifting mechanism for 1h every 2.5h, lifting water flow by the air lifting mechanism to realize that the submerged chlorella floats to the upper layer along with water, keeping the illumination intensity of 3000-8000lx from the microalgae culture barrel by a 40 watt energy-saving lamp, and simultaneously keeping the temperature in the microalgae culture barrel at 23-27 ℃ and regulating and controlling the temperature exceeding the set temperature by 1 ℃;
step three: culturing rotifers: placing the precultured rotifer into a culture tank, and inoculating with density of 1-2 g/ml, the density of the culture in the second introduction step is not less than 3X10 7 The method comprises the steps of (1) regulating microalgae liquid per ml, keeping the water temperature at the fluctuation of 10 ℃ to be not more than 2 ℃, operating a central control system to control and start an oxygenerator, filling pure oxygen into a culture tank through nano-air stones, providing a high-oxygen environment for the growth of rotifers, starting 3-5 times per day, starting 0.5h each time, setting the central control system according to the growth condition of the rotifers, regularly extracting the microalgae liquid from a microalgae culture tank after oxygenation every day, keeping the flow of water in the culture tank through a circulating water pump, controlling a liquid level regulating pipe to keep the water level in the culture tank, and collecting the microalgae liquid when the culture time is 10-20 d according to the density of the rotifers and reaches a target density;
step four: wheel worm collection: the method comprises the steps that when the density of the cultured groove rotifers reaches more than 250/ml, rough estimation of approximate density can be adopted for visual observation by sampling or sampling is carried out through water quality analysis, the target density is achieved, the salinity regulator is driven to reduce salinity, the stimulated rotifers promote massive production of dormant eggs, a motor can be directly driven to work, the rotifers and the dormant eggs are collected through a collection assembly of the rotifers arranged at the bottom, and water in the cultured groove is filtered through a micro-filter and led out to a disinfection sedimentation barrel;
step five: and (3) circularly culturing: the culture solution in the culture operation is filtered by a micro-filter, the culture solution is kept stand and precipitated for 1 day and can be used for secondary culture of rotifers, the treated culture solution is led into a culture tank, then the dormant eggs collected in the step four are added according to the step three operation, the dormant eggs are stimulated to hatch and circulate the step three and the step four, the same culture solution is used for 3-4 times at most, and the culture solution must be replaced by a part of new culture solution to be added continuously through a water inlet to be used as supplement, wherein the replacement amount of the culture solution is generally 15% -25% in specific cases.
As a further setting of the scheme, the culture solution of the microalgae can be F/2 formula culture medium solution.
As a further setting of the scheme, the central control system mainly controls water temperature, salinity, oxygen content and water level standards, and controls the circulating water pump to keep water freshness.
The invention has the following outstanding advantages:
the method is simple to operate, energy-saving and efficient, culture conditions are stable, continuous and cyclic culture can be realized, the indoor culture manual control water temperature and an environment artificial light source can continuously culture targets regardless of external environments, the whole process of simply culturing raw materials does not need too much extra investment, and the whole process comprises the whole process of supplying rotifer baits.
Drawings
FIG. 1 is a schematic diagram of the device of the present invention.
Reference numerals: 1. a central control system; 2. a cultivation tank; 3. a flow meter; 4. microalgae cultivation barrel; 5. a gas distributor; 6. a water outlet A; 7. an outlet conduit; 8. disinfecting the sedimentation barrel; 9. a micro-filter; 91. a water outlet B; 92. a drainage pipe; 10. a nano gas stone tube; 11. a liquid level adjusting pipe; 12. an air lift mechanism; 13. a water inlet; 14. a carbon dioxide generator; 15. a collection assembly for the wheel worms; 16. an oxygenerator; 18. an air inlet.
Detailed Description
The following detailed description of the embodiments of the invention is provided for purposes of illustration and is not to be construed as limiting the invention, as the particular techniques or conditions are not identified in the examples, are described in the literature in this field or are described in terms of product specifications, and the reagents or instruments used are conventional products which are commercially available.
The culture device of the high-density rotifer shown in fig. 1 is characterized by comprising a central control system (1), and a microalgae culture barrel 4 and a culture tank 2 which are controlled by the central control system 1;
the central control system 1 comprises a plurality of sensors arranged in the microalgae cultivation tank 4 and the cultivation tank 2, an oxygenerator 16 for producing pure oxygen and a carbon dioxide generator 14 for producing carbon dioxide, wherein the oxygenerator 16 and the carbon dioxide generator 14 are respectively connected with the nano gas stone pipes 10 arranged in the cultivation tank 2 and the microalgae cultivation tank 4;
the microalgae cultivation barrel 4 comprises a constant temperature device and a salinity regulator which are connected with the central control system 1, an outlet pipeline 7 with a flowmeter is arranged at the bottom of the microalgae cultivation barrel, the microalgae cultivation barrel 4 is further provided with an air lifting mechanism 12, the air lifting mechanism 12 is provided with a gas distributor 5 which stretches into the bottom of the microalgae cultivation barrel 4, and the outlet pipeline 7 is further connected with a water inlet A13 of the cultivation tank 2;
Further, the central control system 1 further comprises a plurality of water pumps.
Further, a rotary mesh for filtering and collecting is arranged in the wheel worm collecting assembly 15, and the mesh aperture is 80-100 microns.
Example 1: indoor rotifer culture based on the device comprises the following steps:
step one: pretreatment of a wheel worm culture device: cleaning the culture device with clear water, exposing the culture device to the sun for 2d under the outdoor sun condition, taking back to the heat preservation greenhouse, standing for 1d and the like, and cooling;
step two: microalgae cultivation: introducing microalgae culture solution into a microalgae culture barrel through a water inlet, inoculating the microalgae which is actively cultured and selected in advance and has no pollution, setting parameters of indoor microalgae culture through a central control system, namely starting a carbon dioxide generator for 0.5h every 3h to provide basic conditions for microalgae photosynthesis, starting an air lifting mechanism for 1h every 2.5h, lifting water flow by the air lifting mechanism to realize that the submerged chlorella floats to the upper layer along with water, keeping the illumination intensity of 3000-8000lx from the microalgae culture barrel by a 40 watt energy-saving lamp, and simultaneously keeping the temperature in the microalgae culture barrel at 23-27 ℃ and regulating and controlling the temperature exceeding the set temperature by 1 ℃;
step three: culturing rotifers: placing the precultured rotifer into a culture tank, inoculating with density of 1-2 g/ml, and introducing into the culture step II to culture with density not less than 3X10 7 The method comprises the steps of (1) regulating microalgae liquid per ml, keeping the water temperature at the fluctuation of 10 ℃ to be not more than 2 ℃, operating a central control system to control and start an oxygenerator, filling pure oxygen into a culture tank through nano-air stones, providing a high-oxygen environment for the growth of rotifers, starting 3-5 times per day, starting 0.5h each time, setting the central control system according to the growth condition of the rotifers, regularly extracting the microalgae liquid from a microalgae culture tank after oxygenation every day, keeping the flow of water in the culture tank through a circulating water pump, controlling a liquid level regulating pipe to keep the water level in the culture tank, and collecting the microalgae liquid when the culture time is 10-20 d according to the density of the rotifers and reaches a target density;
step four: wheel worm collection: the method comprises the steps that when the density of the cultured groove rotifers reaches more than 250/ml, rough estimation of approximate density can be adopted for visual observation by sampling or sampling is carried out through water quality analysis, the target density is achieved, the salinity regulator is driven to reduce salinity, the stimulated rotifers promote massive production of dormant eggs, a motor can be directly driven to work, the rotifers and the dormant eggs are collected through a collection assembly of the rotifers arranged at the bottom, and water in the cultured groove is filtered through a micro-filter and led out to a disinfection sedimentation barrel;
step five: and (3) circularly culturing: the culture solution in the culture operation is filtered by a micro-filter, the culture solution is kept stand and precipitated for 1 day and can be used for secondary culture of rotifers, the treated culture solution is led into a culture tank, then the dormant eggs collected in the step four are added according to the step three operation, the dormant eggs are stimulated to hatch and circulate the step three and the step four, the same culture solution is used for 3-4 times at most, and the culture solution must be replaced by a part of new culture solution to be added continuously through a water inlet to be used as supplement, wherein the replacement amount of the culture solution is generally 15% -25% in specific cases.
Preferably, the culture solution of the microalgae can be F/2 formula culture medium solution.
Preferably, the central control system mainly controls water temperature, salinity, oxygen content and water level standard, and controls the circulating water pump to keep water freshness.
The high-density rotifer cultivated by the scheme is more suitable for being used as bait supply of the fries, improves the nutrition supply of the fries and improves the survival rate of the fries.
The foregoing description is only of the preferred embodiments of the invention, but it should be understood that the above description should not be taken as limiting the invention, and any modifications, equivalents, improvements, etc. within the design concept of the invention should be included in the scope of protection of the invention.
Claims (5)
1. The culture method of the high-density rotifer is characterized in that the adopted culture device comprises a central control system (1), and a microalgae culture barrel (4) and a culture tank (2) which are controlled by the central control system (1);
the central control system (1) comprises a plurality of sensors arranged in the microalgae cultivation barrel (4) and the cultivation tank (2), and also comprises an oxygen generator (16) for producing pure oxygen and a carbon dioxide generator (14) for producing carbon dioxide, wherein the oxygen generator (16) and the carbon dioxide generator (14) are respectively connected with a nano gas stone pipe (10) arranged in the cultivation tank (2) and the microalgae cultivation barrel (4);
the microalgae cultivation barrel (4) comprises a constant temperature device and a salinity regulator which are connected with the central control system (1), an outlet pipeline (7) with a flowmeter is arranged at the bottom of the microalgae cultivation barrel, the microalgae cultivation barrel (4) is further provided with an air lifting mechanism (12), the air lifting mechanism (12) is provided with a gas distributor (5) which stretches into the bottom of the microalgae cultivation barrel (4), and the outlet pipeline (7) is further connected with a water inlet A (13) of the cultivation tank (2);
the cultivation tank (2) comprises a constant temperature device, a salinity regulator and a liquid level regulating pipe (11) which are connected with the central control system (1), a collection assembly (15) for the rotifers is arranged in the cultivation tank (2), the cultivation tank (2) further comprises a water outlet (91) arranged at the bottom, the water outlet (91) is opposite to the micro-filter (9) arranged, the micro-filter (9) is tightly attached to the cultivation tank (2), the micro-filter (9) comprises a drainage pipeline (92), and a disinfection sedimentation barrel (8) is connected and arranged through the drainage pipeline (92);
the method also comprises the following culture steps:
step one: pretreatment of a wheel worm culture device: cleaning the culture device with clear water, exposing the culture device to the sun for 2d under the outdoor sun condition, taking back to the heat preservation greenhouse, standing for 1d and the like, and cooling;
step two: microalgae cultivation: introducing microalgae culture solution into a microalgae culture barrel through a water inlet, inoculating the microalgae which is actively cultured and selected in advance and has no pollution, setting parameters of indoor microalgae culture through a central control system, namely starting a carbon dioxide generator for 0.5h every 3h to provide basic conditions for microalgae photosynthesis, starting an air lifting mechanism for 1h every 2.5h, lifting water flow by the air lifting mechanism to realize that the submerged chlorella floats to the upper layer along with water, keeping the illumination intensity of 3000-8000lx from the microalgae culture barrel by a 40 watt energy-saving lamp, and simultaneously keeping the temperature in the microalgae culture barrel at 23-27 ℃ and regulating and controlling the temperature exceeding the set temperature by 1 ℃;
step three: culturing rotifers: placing the precultured rotifer into a culture tank, inoculating at a density of 1-2/ml, and introducing into the culture step II to culture at a density of not less than 3X10 7 The method comprises the steps of (1) regulating microalgae liquid per ml, keeping the water temperature at the fluctuation of 10 ℃ to be not more than 2 ℃, operating a central control system to control and start an oxygenerator, filling pure oxygen into a culture tank through nano-air stones, providing a high-oxygen environment for the growth of rotifers, starting 3-5 times per day, starting 0.5h each time, setting the central control system according to the growth condition of the rotifers, regularly extracting the microalgae liquid from a microalgae culture tank after oxygenation every day, keeping the flow of water in the culture tank through a circulating water pump, controlling a liquid level regulating pipe to keep the water level in the culture tank, and collecting the microalgae liquid when the culture time is 10-20 d according to the density of the rotifers and reaches a target density;
step four: wheel worm collection: the method comprises the steps that when the density of the cultured groove rotifers reaches more than 250/ml, rough estimation of approximate density can be adopted for visual observation by sampling or sampling is carried out through water quality analysis, the target density is achieved, the salinity regulator is driven to reduce salinity, the stimulated rotifers promote massive production of dormant eggs, a motor can be directly driven to work, the rotifers and the dormant eggs are collected through a collection assembly of the rotifers arranged at the bottom, and water in the cultured groove is filtered through a micro-filter and led out to a disinfection sedimentation barrel;
step five: and (3) circularly culturing: the micro-filter filters the culture solution in the culture tank, the culture solution is placed for precipitation for 1 day and can be used for secondary cultivation of rotifers, the treated culture solution is led into the culture tank, then the dormant eggs collected in the step four are added according to the step three operation, the dormant eggs are stimulated to hatch and circulate the step three and the step four, the same culture solution is used for 3-4 times at most, the culture solution must be replaced, during the use, part of the new culture solution is continuously added through a water inlet to be used as supplement, and the replacement amount of the culture solution is generally 15% -25% according to the specific conditions.
2. The method for culturing high-density rotifers according to claim 1, wherein the central control system (1) further comprises a plurality of water pumps.
3. A method of cultivating high density rotifers as claimed in claim 1, wherein the rotifer collection assembly (15) is provided with a rotating mesh for filtration collection, the mesh aperture being 80-100 microns.
4. The method according to claim 1, wherein the culture medium of microalgae is selected from F/2 formula culture medium.
5. The culture method of high-density rotifers according to claim 1, wherein the central control system mainly controls water temperature, salinity, oxygen content and water level standard, and controls the circulating water pump to keep water fresh.
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| CN111758623A (en) * | 2020-07-10 | 2020-10-13 | 海南德益丰水产科技有限公司 | Method and system for high-density culture of clean rotifers |
| CN112586454A (en) * | 2020-12-15 | 2021-04-02 | 青海珠峰冬虫夏草工程技术研究有限公司 | Large-scale breeding method for cordyceps sinensis host hepialus armoricanus larvae |
| CN112772478A (en) * | 2021-01-12 | 2021-05-11 | 曹礼刚 | Culture method of rotifers |
| CN112997949B (en) * | 2021-03-03 | 2023-05-05 | 赵福振 | Fish nutrient solution culture device rich in bioactive nano organic zinc selenium |
| CN113197133A (en) * | 2021-05-13 | 2021-08-03 | 珠江水利委员会珠江水利科学研究院 | A integration culture apparatus for zooplankton hatching breeds |
| CN113396843A (en) * | 2021-07-12 | 2021-09-17 | 海南绿藻世界生物科技有限公司 | Culture medium rich in microalgae and method for culturing L-type rotifers |
| CN115445274B (en) * | 2022-07-19 | 2024-02-02 | 中国水产科学研究院南海水产研究所 | Device and method for separating and collecting zooplankton metabolism emission organic carbon |
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