CN110100773B - Rapid propagation method for Brachionus plicatilis - Google Patents
Rapid propagation method for Brachionus plicatilis Download PDFInfo
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- CN110100773B CN110100773B CN201910225383.5A CN201910225383A CN110100773B CN 110100773 B CN110100773 B CN 110100773B CN 201910225383 A CN201910225383 A CN 201910225383A CN 110100773 B CN110100773 B CN 110100773B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/20—Culture of aquatic animals of zooplankton, e.g. water fleas or Rotatoria
Abstract
The invention discloses a rapid propagation method for Brachionus plicatilis, which comprises the following steps: the cultivation container, the feeding mode, the filtering mode, the rotifer cultivation, the rotifer collection and the daily management, wherein the cultivation container is a glass fiber jar cultivation barrel with the volume of 1500-2000L, the height of the cultivation barrel is 1.0-1.2m, the bottom of the cultivation barrel is conical and is externally connected with a drain pipe, and the drain pipe is connected with a variable tee joint; the cultivating barrel is provided with an annular temperature control pipe, a nanometer oxygenation pipe which can be communicated with pure oxygen and a common aeration pipe which is communicated with air; the temperature of the culture water is kept between 27 and 30 ℃; the seawater is sand filtered and added into a cultivating barrel, the dissolved oxygen is kept to be more than or equal to 4mg/L in the cultivating process, the salinity is controlled to be 20-25 by adding fresh water, and the pH value is 7.5-8.0. The rapid propagation method for Brachionus plicatilis disclosed by the invention has the advantages that the process is greatly simplified, the workload and the production cost are reduced, the cultivated Rochionus plicatilis has strong fertility, the growth speed is high, the nutrition is sufficient, and the production requirements can be met.
Description
Technical Field
The invention relates to the technical field of rotifer breeding, in particular to a rapid propagation method for Brachionus plicatilis.
Background
Brachionus plicata (Brachionus plicata) exists in fresh water, brackish water and seawater areas, has the characteristics of high propagation speed, rich nutrition, proper size, easiness in culture and the like, and is an ideal initial feed for the fry. However, the artificial cultivation of the rotifers still has low cultivation density and insufficient nutrition, particularly, the rotifers cultivated by yeast have weak fertility and low content of unsaturated fatty acid (EPA and DHA), and nutrition reinforcement is required before feeding. The existing high-density rotifer breeding method is high in equipment investment cost and complex in operation, and is not suitable for small-sized breeding enterprises.
The technical problem to be solved by the technical personnel in the field is to provide a rapid propagation method of Brachionus plicatilis, which has the advantages of simple process, reduced workload and production cost, strong reproductive capacity of the cultivated Rochionus plicatilis, rapid growth speed and sufficient nutrition and can meet the production requirements.
Disclosure of Invention
Technical problem to be solved
The invention provides a rapid propagation method for Brachionus plicatilis, and solves the problems of high propagation cost and complex operation of the conventional Brachionus plicatilis.
(II) technical scheme
In order to solve the technical problems, the invention provides the following technical scheme: a rapid propagation method for Brachionus plicatilis comprises the following steps:
(1) cultivation container
The cultivation container is a glass fiber jar cultivation barrel with the volume of 1500-2000L, the height of the cultivation barrel is 1.0-1.2m, the bottom of the cultivation barrel is conical and is externally connected with a drain pipe, and the drain pipe is connected with a reducing tee; the cultivating barrel is provided with an annular temperature control pipe, a nanometer oxygenation pipe which can be communicated with pure oxygen and a common aeration pipe which is communicated with air; the temperature of the culture water is kept between 27 and 30 ℃; filtering seawater with sand, adding into a cultivating barrel, maintaining dissolved oxygen at 4mg/L or more, adding fresh water, controlling salinity at 20-25, and adjusting pH to 7.5-8.0;
(2) feeding mode
The bait fed by the rotifers is concentrated single-cell green algae with the concentration of 200 hundred million cells/ml, the concentrated single-cell green algae is filled in a black algae barrel, a layer of heat-insulating film is wrapped outside the algae barrel, the volume is 40 liters, the temperature in the algae barrel is kept to be lower than 8 ℃, and the food intake of 1 hundred million rotifers per day is 200ml of concentrated single-cell green algae; one end of the peristaltic pump is connected with the algae barrel, the other end of the peristaltic pump is connected with the rotifer cultivation barrel, the titration frequency of the peristaltic pump is set to be 1.2 s/time, 1.5L of concentrated single-cell green algae can be titrated per hour, and the titration point is fixed above the air stone;
(3) filtration mode
Fixing rectangular filter cotton at the edge of the cultivating barrel, wherein the filter cotton is 5cm higher than the water surface; an air lifting device is arranged outside the cultivating barrel, a common aeration stone is arranged at the bottom of the drain pipe, the aeration stone is aerated and mixed by air and seawater to form air mixed liquid with density lower than that of the original liquid, and the air mixed liquid with low density rises through a pipeline and is discharged into the filter cotton;
(4) rotifer breeding
The temperature of the cultivation water is kept between 27 and 30 ℃, the dissolved oxygen is kept to be more than or equal to 4mg/L in the cultivation process, the illumination intensity is 1500lux, and the illumination time is 18 h; the feeding amount of concentrated single-cell green algae is 200mL per hundred million rotifers every day; by adding fresh water, the salinity is controlled to be 20-25, and the pH value is 7.5-8.0;
when the inoculation density is 250/mL-500/mL and the daily growth rate is 63.5 +/-5.9% -50.2 +/-4.1%, the aeration is carried out through an aeration pipe;
when the inoculation density is 750/mL-1000/mL and the daily growth rate is 32.4 +/-3.5-41.8 +/-3.8%, oxygen is added and pure oxygen is introduced through an aerator pipe;
(5) rotifer collection
A rotifer collecting net is placed in the collecting barrel, a filter screen and filter cotton are placed on the rotifer collecting net and used for filtering metabolites and food residues of the rotifers, and a ball valve at the bottom of the cultivating barrel is opened to start collecting; the collecting net is shifted from time to time in the collecting process, so that water in the collecting net flows out without overflowing; after collection, the filter cotton, the filter screen and the collection net are washed clean by a high-pressure water gun; the collected rotifers can be directly fed or subjected to expanded culture;
(6) daily management
Adding concentrated unicellular green algae into the culture tank once a day, turning over once a day, adjusting the temperature, salinity and other indexes in advance by using a new culture tank, and inoculating the collected rotifers according to the required inoculation density; water temperature, pH and salinity were measured daily.
Preferably, the rotifers are collected in 3 times when the number of the rotifers is large.
Preferably, the temperature of the water in the incubation container is adjusted by an annular temperature control tube.
Preferably, the drain pipe is a PVC pipe with a nominal outer diameter of 50mm, and the size of the reducing tee joint is 20 mm.
Preferably, the chlorella in the algae bucket in the feeding mode is cooled by an ice bag.
Preferably, the filter cotton is 0.3m long, 0.3m wide, 1m high, 0.5cm thick and 100um aperture.
(III) advantageous effects
The rapid propagation method for Brachionus plicatilis disclosed by the invention has the advantages that the process is greatly simplified, the workload and the production cost are reduced, the cultivated Rochionus plicatilis has strong fertility, the growth speed is high, the nutrition is sufficient, and the production requirements can be met.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The invention provides a rapid propagation method of Brachionus plicatilis, which comprises the following steps:
1) cultivation container
The cultivation container is a glass fiber jar cultivation barrel with the volume of 1500-2000L, the barrel bottom is conical, the height of the cultivation barrel is 1.0-1.2m, the bottom is externally connected with a drainage pipe of a PVC pipe with the nominal outer diameter of 50mm, and the drainage pipe is connected with a reducing tee with the diameter of 20 mm; the cultivating barrel is provided with an annular temperature control pipe, a nanometer oxygenation pipe which can be communicated with pure oxygen and a common aeration pipe which is communicated with air; the temperature of the cultivation water is kept at 27-30 ℃, the water temperature can be adjusted through an annular temperature control pipe, and the annular temperature control pipe is large in heating area and uniform in heating; filtering seawater with sand, adding into a cultivating barrel, maintaining dissolved oxygen at 4mg/L or more, adding fresh water, controlling salinity at 20-25, and adjusting pH to 7.5-8.0;
(2) feeding mode
The bait for cultivating the rotifer is concentrated single-cell green algae with the concentration of 200 hundred million cells/ml, the concentrated single-cell green algae is arranged in a black algae barrel, a layer of heat-insulating film is wrapped outside the black algae barrel, the volume of the black algae barrel is 40 liters, the chlorella in the algae barrel needs to be put in an ice bag to reduce the temperature, the temperature in the barrel is kept to be lower than 8 ℃, and the daily food intake of 1 hundred million rotifers is 200ml of concentrated chlorella; one end of the peristaltic pump is connected with the algae barrel, and the other end of the peristaltic pump is connected with the rotifer cultivating barrel; setting the titration frequency of the peristaltic pump to be 1.2 s/time, titrating 1.5L of concentrated unicellular green algae per hour, and fixing the titration point above the air stone to disperse the added algae liquid in time;
(3) filtration mode
Placing cuboid filter cotton in a cultivating barrel, wherein the periphery and bottom of the filter cotton are all made of filter cotton with the thickness of 0.5cm and the aperture of 100um, the filter cotton is 0.3m long, 0.3m wide and 1m high, is fixed at the edge of the barrel and is about 5cm higher than the water surface; the method is characterized in that an air lifting device is arranged outside a cultivating barrel, a common aeration stone is placed at the bottom of a PVC pipe with the nominal outer diameter of 50mm, air and seawater are mixed to form air mixed liquid with the density lower than that of original liquid, the mixed liquid with the low density rises through a pipeline and is discharged into filter cotton, so that a small circulation effect can be formed, and metabolites and food residues of rotifers are effectively filtered through circulation of rotifers in a cultivating water body;
the ammonia nitrogen has toxic action on the growth of the rotifer, influences the growth and reproduction speed, and the content of the ammonia nitrogen (NH3-N) in the water body can be reduced by increasing the gas stripping device (because metabolites and food residues of the rotifer are filtered by the interception action).
24h cultivation experiment: the inoculation density of the rotifer is 500/mL, and the ammonia nitrogen concentration in the water body after 24 hours of cultivation is 3.0 +/-0.28 mg/L; and (3) adding a gas stripping device, and after 24 hours, culturing the water body with ammonia nitrogen concentration of 2.3 +/-0.22 mg/L and reducing the ammonia nitrogen content by about 23.3%.
(4) Culture of rotifer
The temperature of the cultivation water is kept between 27 and 30 ℃, the Dissolved Oxygen (DO) is kept to be more than or equal to 4mg/L in the cultivation process, the illumination intensity is 1500lux, and the illumination time is 18 h; the feeding amount of concentrated single-cell green algae is 200mL per hundred million rotifers every day; by adding fresh water, the salinity is controlled to be 20-25, and the pH value is 7.5-8.0;
meanwhile, according to different densities, whether pure oxygen is introduced or not can be selected:
the inoculation density is 250 pieces/mL, and the daily growth rate is 63.5 +/-5.9%. The common aeration pipe increases oxygen;
the inoculation density is 500 pieces/mL, and the daily growth rate is 50.2 +/-4.1%. The common aeration pipe increases oxygen;
the inoculation density is 750/mL, and the daily growth rate is 41.8 +/-3.8%. Aerating and introducing pure oxygen through an aeration pipe;
the inoculation density is 1000 pieces/mL, and the daily growth rate is 32.4 +/-3.5%. The aeration pipe increases oxygen and is filled with pure oxygen.
(5) Rotifer collection
One side of the collecting barrel is punched to control the water level height, the aperture of a rotifer collecting net is 48 mu m, a filter screen for bearing and filter cotton with the aperture of 100 mu m are placed on the collecting net to filter metabolites and food residues of the rotifers, when the collection is started, a ball valve at the bottom of the cultivating barrel is opened, when the rotifers are large in number, the rotifers are collected for 3 times, and the rotifers cannot be collected for too long, otherwise, the rotifers on the collecting net are high in density and are easy to die; the collecting net is shifted from time to time in the collecting process, so that water in the collecting net flows out without overflowing; after collection, the filter cotton, the filter screen and the collection net are washed clean by a high-pressure water gun; the collected rotifers can be directly fed or subjected to expanded culture;
(6) daily management
Adding concentrated unicellular green algae into the culture tank once a day, and turning over once a day, wherein the temperature, salinity and other indexes of the new culture tank are adjusted in advance, and inoculating the collected rotifers according to the required inoculation density; water temperature, pH and salinity were measured daily.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (6)
1. A rapid propagation method for Brachionus plicatilis is characterized by comprising the following steps:
(1) cultivation container
The cultivation container is a glass fiber jar cultivation barrel with the volume of 1500-2000L, the height of the cultivation barrel is 1.0-1.2m, the bottom of the cultivation barrel is conical and is externally connected with a drain pipe, and the drain pipe is connected with a reducing tee; the cultivating barrel is provided with an annular temperature control pipe, a nano oxygenation pipe for introducing pure oxygen and a common aeration pipe for introducing air; the temperature of the culture water is kept at 27 ℃; filtering seawater with sand, adding into a cultivating barrel, maintaining dissolved oxygen at 4mg/L during cultivating, adding fresh water, controlling salinity at 25, and adjusting pH to 7.5-8.0;
(2) feeding mode
The bait fed by the rotifers is concentrated single-cell green algae with the concentration of 200 hundred million cells/ml, the concentrated single-cell green algae is filled in a black algae barrel, a layer of heat-insulating film is wrapped outside the algae barrel, the volume is 40 liters, the temperature in the algae barrel is kept to be lower than 8 ℃, and the food intake of 1 hundred million rotifers per day is 200ml of concentrated single-cell green algae; one end of the peristaltic pump is connected with the algae barrel, the other end of the peristaltic pump is connected with the rotifer cultivation barrel, the titration frequency of the peristaltic pump is set to be 1.2 s/time, 1.5L of concentrated unicellular green algae liquid is titrated per hour, and the titration point is fixed above the air stone;
(3) filtration mode
Fixing rectangular filter cotton at the edge of the cultivating barrel, wherein the filter cotton is 5cm higher than the water surface; an air lifting device is arranged outside the cultivating barrel, a common aeration stone is arranged at the bottom of the drain pipe, the aeration stone is aerated and mixed by air and seawater to form air mixed liquid with density lower than that of the original liquid, and the air mixed liquid with low density is lifted through a pipeline and discharged into the filter cotton;
(4) rotifer breeding
The temperature of the cultivation water is kept at 27 ℃, the dissolved oxygen is kept at 4mg/L in the cultivation process, the illumination intensity is 1500lux, and the illumination time is 18 h; the feeding amount of concentrated single-cell green algae is 200mL per hundred million rotifers every day; by adding fresh water, the salinity is controlled to be 25, and the pH value is 7.5-8.0;
when the inoculation density is 250/mL-500/mL and the daily growth rate is 63.5 +/-5.9% -50.2 +/-4.1%, the aeration is carried out through an aeration pipe;
when the inoculation density is 750/mL-1000/mL and the daily growth rate is 32.4 +/-3.5-41.8 +/-3.8%, oxygen is added and pure oxygen is introduced through an aerator pipe;
(5) rotifer collection
A rotifer collecting net is placed in the collecting barrel, a filter screen and filter cotton are placed on the rotifer collecting net and used for filtering metabolites and food residues of the rotifers, and a ball valve at the bottom of the cultivating barrel is opened to start collecting; the collecting net is shifted from time to time in the collecting process, so that water in the collecting net flows out without overflowing; after collection, the filter cotton, the filter screen and the collection net are washed clean by a high-pressure water gun; directly feeding the collected rotifers or carrying out expanded culture;
(6) daily management
Adding concentrated unicellular green algae into the cultivating barrel once a day, turning the cultivating barrel once a day, adjusting the proper temperature and salinity indexes in advance by a new cultivating barrel, and inoculating the collected rotifers according to the required inoculation density; water temperature, pH and salinity were measured daily.
2. The rapid propagation method of Brachionus plicatilis according to claim 1, comprising the following steps: when the number of the rotifers is large, the rotifers are collected by 3 times.
3. The rapid propagation method of Brachionus plicatilis according to claim 1, comprising the following steps: the temperature of water in the culture container is adjusted through an annular temperature control pipe.
4. The rapid propagation method of Brachionus plicatilis according to claim 1, comprising the following steps: the drain pipe is a PVC pipe with a nominal outer diameter of 50mm, and the size of the reducing tee joint is 20 mm.
5. The rapid propagation method of Brachionus plicatilis according to claim 1, comprising the following steps: the temperature of the chlorella in the algae bucket is reduced by placing an ice bag in the feeding mode.
6. The rapid propagation method of Brachionus plicatilis according to claim 1, comprising the following steps: the filter cotton is 0.3m long, 0.3m wide and 1m high, 0.5cm thick and 100um aperture.
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| CN111248139B (en) * | 2020-03-27 | 2023-06-20 | 浙江省海洋水产养殖研究所 | Culture device and method for high-density rotifers |
| CN111528147A (en) * | 2020-05-21 | 2020-08-14 | 中国水产科学研究院东海水产研究所 | Small water body high-density rotifer cultivation method |
| CN111758623A (en) * | 2020-07-10 | 2020-10-13 | 海南德益丰水产科技有限公司 | Method and system for high-density culture of clean rotifers |
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| CN113396843A (en) * | 2021-07-12 | 2021-09-17 | 海南绿藻世界生物科技有限公司 | Culture medium rich in microalgae and method for culturing L-type rotifers |
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