CN110100773A - A kind of brachionus plicatilis quick propagation method - Google Patents
A kind of brachionus plicatilis quick propagation method Download PDFInfo
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- CN110100773A CN110100773A CN201910225383.5A CN201910225383A CN110100773A CN 110100773 A CN110100773 A CN 110100773A CN 201910225383 A CN201910225383 A CN 201910225383A CN 110100773 A CN110100773 A CN 110100773A
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- bucket
- wheel animalcule
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- temperature
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- 238000000034 method Methods 0.000 title claims abstract description 32
- 241000700104 Brachionus plicatilis Species 0.000 title claims abstract description 19
- 241000700141 Rotifera Species 0.000 claims abstract description 52
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 25
- 238000005273 aeration Methods 0.000 claims abstract description 17
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000001301 oxygen Substances 0.000 claims abstract description 11
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 11
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims abstract description 9
- 239000013505 freshwater Substances 0.000 claims abstract description 8
- 239000013535 sea water Substances 0.000 claims abstract description 8
- 238000007664 blowing Methods 0.000 claims abstract description 4
- 239000003365 glass fiber Substances 0.000 claims abstract description 4
- 241000195493 Cryptophyta Species 0.000 claims description 23
- 229920000742 Cotton Polymers 0.000 claims description 18
- 239000002054 inoculum Substances 0.000 claims description 12
- 238000006213 oxygenation reaction Methods 0.000 claims description 8
- 238000002156 mixing Methods 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 5
- 230000002572 peristaltic effect Effects 0.000 claims description 5
- 239000004575 stone Substances 0.000 claims description 5
- 238000004448 titration Methods 0.000 claims description 5
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 4
- 239000007789 gas Substances 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 claims description 4
- 230000005574 cross-species transmission Effects 0.000 claims description 3
- 238000002955 isolation Methods 0.000 claims description 3
- 238000004321 preservation Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000013461 design Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 150000003839 salts Chemical class 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 1
- 235000007164 Oryza sativa Nutrition 0.000 claims 1
- 235000009566 rice Nutrition 0.000 claims 1
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 230000035764 nutrition Effects 0.000 abstract description 5
- 230000001850 reproductive effect Effects 0.000 abstract description 4
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 6
- 238000009395 breeding Methods 0.000 description 3
- 230000001488 breeding effect Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/20—Culture of aquatic animals of zooplankton, e.g. water fleas or Rotatoria
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Marine Sciences & Fisheries (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Feed For Specific Animals (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of brachionus plicatilis quick propagation methods, it include: to cultivate container, feed mode, filter type, wheel animalcule cultivation, wheel animalcule collection and daily management, it wherein cultivates the glass fibre cylinder that container is volume 1500-2000L and cultivates bucket, cultivate the high 1.0-1.2m of bucket, cultivating bucket bucket bottom is cone and external drainpipe, connects reducing tee on drainpipe;It cultivates bucket and annular temperature-controlled tube, the common aeration tube equipped with the nanometer oxygen increasing tube and blowing air that can lead to pure oxygen is installed;It cultivates water temperature and is maintained at 27-30 DEG C;Seawater is added to after husky filter and cultivates in bucket, and cultivating process keeps dissolved oxygen >=4mg/L, and by the way that fresh water is added, salinity is controlled in 20-25, pH value 7.5-8.0.The brachionus plicatilis quick propagation method technique of the invention patent greatly simplifies, reduces workload and production cost, and the wheel animalcule reproductive capacity of cultivation is strong, the speed of growth is fast, nutrition is abundant, can satisfy production requirement.
Description
Technical field
The present invention relates to wheel animalcules to cultivate breeding technical field, and in particular to a kind of brachionus plicatilis quick propagation method.
Background technique
Brachionus plicatilis (Brachionus plicatilis) is present in fresh water, brackish water and body of sea water, has numerous
It is fast to grow speed, full of nutrition, suitable size and is easy to the features such as cultivating, is the ideal open-mouthed bait of prelarva.But wheel animalcule is artificial
Cultivate that there are still cultivation densities that low, nutrition is not enough, the wheel animalcule reproductive capacity especially cultivated with yeast is weak, unsaturated lipid
Fat acid (EPA, DHA) content is low, also needs to carry out fortification before feeding.Existing high density culture of rotifera method often throw by equipment
Enter at high cost, cumbersome, is not suitable for small-sized breeding enterprise.
A kind of simple process is provided, reduces workload and production cost, the wheel animalcule reproductive capacity of cultivation is strong, the speed of growth is fast,
Nutrition is abundant, and the brachionus plicatilis quick propagation method that can satisfy production requirement is that those skilled in the art are urgently to be resolved
Technical problem.
Summary of the invention
(1) the technical issues of solving
The present invention provides a kind of brachionus plicatilis quick propagation method, solves existing brachionus plicatilis and expands numerous cost
High, cumbersome problem.
(2) technical solution
In order to solve the above technical problems, the invention provides the following technical scheme: a kind of brachionus plicatilis fast-propagation side
Method, comprising the following steps:
(1) container is cultivated
It cultivates the glass fibre cylinder that container is volume 1500-2000L and cultivates bucket, cultivate the high 1.0-1.2m of bucket, cultivate bucket bucket
Bottom is cone and external drainpipe, connects reducing tee on drainpipe;It cultivates bucket and annular temperature-controlled tube is installed, equipped with pure oxygen can be led to
Nanometer oxygen increasing tube and blowing air common aeration tube;It cultivates water temperature and is maintained at 27-30 DEG C;Seawater is added to training after husky filter
It educates in bucket, cultivating process keeps dissolved oxygen >=4mg/L, and by the way that fresh water is added, salinity is controlled in 20-25, pH value 7.5-8.0;
(2) mode is fed
The bait that culture of rotifera is fed is the concentrated type monoplast green alga that concentration is 20,000,000,000 cells/mls, concentrated type list
Cell green alga is loaded in the algae bucket of black, and algae bucket is external to encase one layer of heat preservation thermal isolation film, and 40 liters of volume, algae bucket bucket is interior to keep temperature
Lower than 8 DEG C, the daily food ration of 100,000,000 wheel animalcules is 200ml concentrated type monoplast green alga;Peristaltic pump one end connects algae bucket, the other end
It connects wheel animalcule and cultivates bucket, be 1.2s/ times by the titration set of frequency of peristaltic pump, the concentration algae solution of 1.5L can be titrated per hour,
Titration point is fixed on gas Shi Shangfang;
(3) filter type
The filter cotton of the fixed rectangular-shape in edge, filter cotton are higher by water surface 5cm in cultivating bucket;Gas is designed outside bucket cultivating
Device is mentioned, places a common aeration stone in draining bottom of the tube, aeration stone is mixed to form using air with seawater aeration closeer than stoste
Low air mixing liquid is spent, the small air mixing liquid of density is risen to by pipeline to be discharged in filter cotton;
(4) wheel animalcule is cultivated
It cultivates water temperature and is kept for 27-30 DEG C, cultivating process keeps dissolved oxygen >=4mg/L, intensity of illumination 1500lux, illumination
Time 18h;The feeding volume of concentration algae solution is every hundred million wheel animalcule of 200mL daily;By the way that fresh water is added, salinity is controlled in 20-25, pH value
For 7.5-8.0;
As 250/mL-500/mL of inoculum density, 63.5 ± 5.9%-50.2 of daily progression rate ± 4.1%, pass through exposure
Tracheae oxygenation;
As 750/mL-1000/mL of inoculum density, 32.4 ± 3.5%-41.8 of daily progression rate ± 3.8%, pass through exposure
Tracheae oxygenation and logical pure oxygen;
(5) wheel animalcule is collected
Collecting vessel places wheel animalcule collecting net, filter screen and filter cotton is placed on wheel animalcule collecting net, for filtering the generation of wheel animalcule
Object and swill are thanked, wheel animalcule bucket bottom ball valve is opened, starts to collect;Collection process will stir collecting net from time to time, make to receive
Water outflow in collection net, does not spill over;After having collected, filter cotton, filter screen and collecting net are rinsed well with giant;It gathers
Wheel animalcule directly can feed or expand culture;
(6) daily management
Culture bucket adds primary concentration algae solution daily, and bucket is primary daily, and new culture bucket will regulate suitable temperature in advance
The inoculum density of the wheel animalcule of collection as required is inoculated with by the indexs such as degree, salinity;Water temperature, pH value, salt are measured daily
Degree.
Preferably, the wheel animalcule is collected when wheel animalcule quantity is more, point 3 collections.
Preferably, water temperature passes through annular temperature-controlled tube adjusting in the cultivation container.
Preferably, the drainpipe is the pvc pipe of nominal outside diameter 50mm, and the size of reducing tee is 20mm.
Preferably, described to feed in mode chlorella in algae bucket and reduce temperature by putting ice bag.
Preferably, the long 0.3m of the filter cotton, width 0.3m, high 1m, thickness 0.5cm, aperture 100um.
(3) beneficial effect
The brachionus plicatilis quick propagation method technique of the invention patent greatly simplifies, reduces workload and be produced into
This, the wheel animalcule reproductive capacity of cultivation is strong, the speed of growth is fast, nutrition is abundant, can satisfy production requirement.
Specific embodiment
Below in conjunction with the embodiment of the present invention, technical scheme in the embodiment of the invention is clearly and completely described,
Obviously, described embodiments are only a part of the embodiments of the present invention, instead of all the embodiments.Based in the present invention
Embodiment, every other embodiment obtained by those of ordinary skill in the art without making creative efforts, all
Belong to the scope of protection of the invention.
A kind of brachionus plicatilis quick propagation method provided by the invention, steps are as follows:
1) container is cultivated
It cultivates the glass fibre cylinder that container is volume 1500-2000L and cultivates bucket, bucket bottom is cone, cultivates the high 1.0- of bucket
1.2m, the drainpipe of the external nominal outside diameter 50mmPVC pipe in bottom connect the reducing tee of 20mm on drainpipe;Bucket is cultivated to be equipped with
Annular temperature-controlled tube, the common aeration tube equipped with the nanometer oxygen increasing tube and blowing air that can lead to pure oxygen;It cultivates water temperature and is kept for 27-30 DEG C,
Water temperature can be adjusted by annular temperature-controlled tube, and annular temperature-controlled tube heating surface area is big, homogeneous heating;Seawater adds after husky filter
To cultivating in bucket, cultivating process keeps dissolved oxygen >=4mg/L, and by the way that fresh water is added, salinity is controlled in 20-25, pH value 7.5-
8.0;
(2) mode is fed
The bait of this method culture of rotifera is the concentrated type monoplast green alga that concentration is 20,000,000,000 cells/mls, concentrated type
For monoplast green alga loaded in the algae bucket of black, outside encases one layer of heat preservation thermal isolation film, and 40 liters of volume, chlorella needs to put in algae bucket
Ice bag reduces temperature, keeps temperature within the barrel to be lower than 8 DEG C, the daily food ration of 100,000,000 wheel animalcules is that chlorella is concentrated in 200ml;It wriggles
One end connection algae bucket is pumped, the other end connects wheel animalcule and cultivates bucket;It is 1.2s/ times by the titration set of frequency of peristaltic pump, per hour may be used
To titrate the concentration algae solution of 1.5L and titration point should be fixed on gas Shi Shangfang, so that the algae solution being added scatter in time;
(3) filter type
The filter cotton of rectangular-shape is placed in cultivating bucket, filter cotton surrounding and bottom are all thickness 0.5cm, and aperture is
The filter cotton of 100um forms, and the long 0.3m of filter cotton, width 0.3m, high 1m are fixed on barrel edge, are higher by the water surface about 5cm;It is cultivating
Bucket is outer to design air-lift device, places a common aeration stone in nominal outside diameter 50mmPVC bottom of the tube, utilizes air and sea water mixing shape
At air mixing liquid more low density than stoste, the small mixed liquor of density is risen to by pipeline to be discharged in filter cotton, thus
A small ringing can be formed, by cultivating wheel animalcule the circulation of water body, has effectively filtered the metabolin and food of wheel animalcule
Object residue;
Ammonia nitrogen has toxic action to the growth of wheel animalcule, influences growth and breeding speed, and water body can be lowered by increasing air-lift device
Middle ammonia nitrogen (NH3-N) content (the reason is that by interception function, having filtered the metabolin and swill of wheel animalcule).
Culture experiment for 24 hours: 500/mL of wheel animalcule inoculum density, cultivate afterwards for 24 hours ammonia nitrogen concentration in water body be 3.0 ±
0.28mg/L;Increase air-lift device, cultivating ammonia nitrogen concentration in water body afterwards for 24 hours is 2.3 ± 0.22mg/L, and ammonia-nitrogen content reduces about
23.3%.
(4) wheel animalcule is cultivated
It cultivates water temperature and is kept for 27-30 DEG C, cultivating process keeps dissolved oxygen (DO) >=4mg/L, intensity of illumination 1500lux, light
According to time 18h;The feeding volume of concentration algae solution is every hundred million wheel animalcules 200mL daily;By the way that fresh water is added, salinity is controlled in 20-25, pH
Value is 7.5-8.0;
Meanwhile according to different density, logical pure oxygen may choose whether:
250/mL of inoculum density, daily progression rate 63.5 ± 5.9%.Common aeration tube oxygenation;
500/mL of inoculum density, daily progression rate 50.2 ± 4.1%.Common aeration tube oxygenation;
750/mL of inoculum density, daily progression rate 41.8 ± 3.8%.Aeration tube oxygenation and logical pure oxygen;
1000/mL of inoculum density, daily progression rate 32.4 ± 3.5%.Aeration tube oxygenation and logical pure oxygen.
(5) wheel animalcule is collected
One side perforating of collecting vessel puts wheel animalcule and collects screen distance as 48um, place and use on collecting net to control height of water level
The filter cotton for being 100um in the filter screen of load-bearing and aperture, filters the metabolin and swill of wheel animalcule, when starting to collect, wheel
Worm bucket bottom ball valve is opened, and when wheel animalcule quantity is more, point 3 collections can not be waited too long, and otherwise collecting net wheel animalcule density is big, is easy
Dead a part;Collection process will stir collecting net from time to time, flow out the water in collecting net, do not spill over;After having collected, mistake
Filter pulp, filter screen and collecting net are rinsed well with giant;The wheel animalcule gathered can directly feed or carry out expansion training
It supports;
(6) daily management
Culture bucket adds primary concentration algae solution daily, and bucket is primary daily, and it is suitable that new culture bucket will regulate in advance
The indexs such as temperature, salinity, the inoculum density of the wheel animalcule of collection as required is inoculated with;To measure daily water temperature, pH value,
Salinity.
It although an embodiment of the present invention has been shown and described, for the ordinary skill in the art, can be with
A variety of variations, modification, replacement can be carried out to these embodiments without departing from the principles and spirit of the present invention by understanding
And modification, the scope of the present invention is defined by the appended.
Claims (6)
1. a kind of brachionus plicatilis quick propagation method, which comprises the following steps:
(1) container is cultivated
It cultivates the glass fibre cylinder that container is volume 1500-2000L and cultivates bucket, cultivate the high 1.0-1.2m of bucket, cultivating bucket bucket bottom is
Conical and external drainpipe connects reducing tee on drainpipe;It cultivates bucket and annular temperature-controlled tube is installed, equipped with receiving for pure oxygen can be led to
The common aeration tube of rice oxygen increasing tube and blowing air;It cultivates water temperature and is maintained at 27-30 DEG C;Seawater is added to cultivation bucket after husky filter
In, cultivating process keeps dissolved oxygen >=4mg/L, and by the way that fresh water is added, salinity is controlled in 20-25, pH value 7.5-8.0;
(2) mode is fed
The bait that culture of rotifera is fed is the concentrated type monoplast green alga that concentration is 20,000,000,000 cells/mls, and concentrated type is unicellular
Green alga is loaded in the algae bucket of black, and algae bucket is external to encase one layer of heat preservation thermal isolation film, and 40 liters of volume, algae bucket bucket is interior to keep temperature to be lower than
8 DEG C, the daily food ration of 100,000,000 wheel animalcules is 200ml concentrated type monoplast green alga;Peristaltic pump one end connects algae bucket, other end connection
Wheel animalcule cultivates bucket, is 1.2s/ times by the titration set of frequency of peristaltic pump, can titrate the concentration algae solution of 1.5L per hour, titrates
Point is fixed on gas Shi Shangfang;
(3) filter type
The filter cotton of the fixed rectangular-shape in edge, filter cotton are higher by water surface 5cm in cultivating bucket;Cultivating design air lift dress outside bucket
It sets, places a common aeration stone in draining bottom of the tube, aeration stone is mixed to form using air with seawater aeration lower than stoste density
Air mixing liquid, the small air mixing liquid of density is risen to by pipeline to be discharged in filter cotton;
(4) wheel animalcule is cultivated
It cultivates water temperature and is kept for 27-30 DEG C, cultivating process keeps dissolved oxygen >=4mg/L, intensity of illumination 1500lux, light application time
18h;The feeding volume of concentration algae solution is every hundred million wheel animalcule of 200mL daily;By the way that fresh water is added, salinity is controlled in 20-25, and pH value is
7.5-8.0;
As 250/mL-500/mL of inoculum density, 63.5 ± 5.9%-50.2 of daily progression rate ± 4.1%, pass through aeration tube
Oxygenation;
As 750/mL-1000/mL of inoculum density, 32.4 ± 3.5%-41.8 of daily progression rate ± 3.8%, pass through aeration tube
Oxygenation and logical pure oxygen;
(5) wheel animalcule is collected
Collecting vessel places wheel animalcule collecting net, filter screen and filter cotton is placed on wheel animalcule collecting net, for filtering the metabolin of wheel animalcule
And swill, wheel animalcule bucket bottom ball valve are opened, start to collect;Collection process will stir collecting net from time to time, make collecting net
In water outflow, do not spill over;After having collected, filter cotton, filter screen and collecting net are rinsed well with giant;The wheel gathered
Worm directly can feed or expand culture;
(6) daily management
Culture bucket adds primary concentration algae solution daily, and bucket is primary daily, and new culture bucket will regulate suitable temperature, salt in advance
The indexs such as degree, the inoculum density of the wheel animalcule of collection as required is inoculated with;Water temperature, pH value, salinity are measured daily.
2. brachionus plicatilis quick propagation method according to claim 1, it is characterised in that: the wheel animalcule collects and taking turns
When borer population amount is more, point 3 collections.
3. brachionus plicatilis quick propagation method according to claim 1, it is characterised in that: water in the cultivation container
Temperature is adjusted by annular temperature-controlled tube.
4. brachionus plicatilis quick propagation method according to claim 1, it is characterised in that: the drainpipe is nominal
The pvc pipe of outer diameter 50mm, the size of reducing tee are 20mm.
5. brachionus plicatilis quick propagation method according to claim 1, it is characterised in that: described to feed algae in mode
Chlorella reduces temperature by putting ice bag in bucket.
6. brachionus plicatilis quick propagation method according to claim 1, it is characterised in that: the filter cotton is long
0.3m, width 0.3m, high 1m, thickness 0.5cm, aperture 100um.
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Cited By (7)
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CN111066703A (en) * | 2020-01-13 | 2020-04-28 | 中国科学院水生生物研究所 | Method for carrying out half-environment accommodation capacity expanding culture on brachionus calyciflorus |
CN111248139A (en) * | 2020-03-27 | 2020-06-09 | 浙江省海洋水产养殖研究所 | Culture device and method for high-density rotifers |
CN111528147A (en) * | 2020-05-21 | 2020-08-14 | 中国水产科学研究院东海水产研究所 | Small water body high-density rotifer cultivation method |
CN111758623A (en) * | 2020-07-10 | 2020-10-13 | 海南德益丰水产科技有限公司 | Method and system for high-density culture of clean rotifers |
CN112119979A (en) * | 2020-08-17 | 2020-12-25 | 浙江海洋大学 | Culture apparatus of light lip fish initial feed |
CN113396843A (en) * | 2021-07-12 | 2021-09-17 | 海南绿藻世界生物科技有限公司 | Culture medium rich in microalgae and method for culturing L-type rotifers |
CN115088669A (en) * | 2022-07-05 | 2022-09-23 | 生态环境部南京环境科学研究所 | Culture apparatus of fold arm tail wheel worm |
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CN112119979A (en) * | 2020-08-17 | 2020-12-25 | 浙江海洋大学 | Culture apparatus of light lip fish initial feed |
CN113396843A (en) * | 2021-07-12 | 2021-09-17 | 海南绿藻世界生物科技有限公司 | Culture medium rich in microalgae and method for culturing L-type rotifers |
CN115088669A (en) * | 2022-07-05 | 2022-09-23 | 生态环境部南京环境科学研究所 | Culture apparatus of fold arm tail wheel worm |
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