CN211932151U - Culture apparatus of high density rotifer - Google Patents

Culture apparatus of high density rotifer Download PDF

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Publication number
CN211932151U
CN211932151U CN202020418737.6U CN202020418737U CN211932151U CN 211932151 U CN211932151 U CN 211932151U CN 202020418737 U CN202020418737 U CN 202020418737U CN 211932151 U CN211932151 U CN 211932151U
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rotifer
culture
control system
central control
microalgae
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闫茂仓
张立宁
邵鑫斌
马建忠
王瑶华
陈然
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Zhejiang Mariculture Research Institute
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Zhejiang Mariculture Research Institute
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Abstract

The utility model discloses a culture apparatus of high density rotifer, including rotifer breeding device, this breeding device includes breeding groove, little algae cultivation bucket, center control system, and this method includes: pretreating a rotifer culture device; culturing microalgae; culturing rotifers; collecting rotifers; circularly culturing; it cultivates the bucket and supplies with carbon dioxide with the speed up little algae growth through the little algae of central control system control, control little algae and cultivate the bucket and carry the high density little algae as bait and fill in the pure oxygen and supply the rotifer growth demand to breeding the groove, and after the rotifer grows to certain density, central control system carries out the blowdown and collects the rotifer to breeding the groove, and the circulation of rotifer culture solution is recycled, this technique realizes the culture apparatus of circulation supply through improving aquatic dissolved oxygen and high density food, high standard, high density rotifer is cultivateed, and has improved rotifer and bred survival rate, breed efficiency.

Description

Culture apparatus of high density rotifer
Technical Field
The utility model relates to a biological cultivation field, more specifically say that it relates to a rotifer breeding device of high density.
Background
The rotifer is a smiling multicellular organism, is rich in three basic nutrients of protein, fat and carbohydrate, and also contains various amino acids, vitamins, highly unsaturated fatty acids and other various bioactive substances. In the field of aquatic organism domestication, the bait is commonly used as living body bait for young seedlings, and because the bait has the advantages of excellent feeding effect, rapid self propagation, strong vitality and proper size, the bait provides the best bait for domesticating aquatic organisms of fishes and shrimps, and is widely applied to aquaculture.
In recent years, protection of ecological aquatic resources and seedling culture are returned to implementation of water resource protection and continuous plan, and increasingly attention is paid to aquaculture of aquatic seedlings, so that development of multiple better and more economical baits is promoted.
SUMMERY OF THE UTILITY MODEL
To the not enough of the above-mentioned existence, the utility model discloses a culture apparatus of high density rotifer adjusts aquaculture environment with central control system, at indoor high density's reproduction rotifer.
In order to achieve the above purpose, the utility model provides a following technical scheme: a culture device for high-density rotifers is characterized by comprising a central control system, a microalgae culture barrel and a culture tank, wherein the microalgae culture barrel and the culture tank are controlled by the central control system;
the central control system comprises a plurality of sensors arranged in the microalgae culture barrel and the culture tank, an oxygen generator for producing pure oxygen and a carbon dioxide generator for producing carbon dioxide, wherein the oxygen generator and the carbon dioxide generator are respectively connected with the nano-aerolith tubes arranged in the culture tank and the microalgae culture barrel;
the microalgae cultivation barrel comprises a constant temperature device and a salinity regulator which are connected with a central control system, an outlet pipeline with a flowmeter is arranged at the bottom of the microalgae cultivation barrel, the microalgae cultivation barrel is also provided with an air-lift mechanism, the air-lift mechanism is provided with a gas distributor which extends into the bottom of the microalgae cultivation barrel, and the outlet pipeline is also connected with a water inlet A of the cultivation tank;
breed the groove including constant temperature equipment, salinity regulator, the liquid level control pipe be connected with central control system, and breed the collection subassembly that the inslot was provided with the rotifer, breed the groove still including setting up delivery port, the delivery port just to the microstrainer that sets up in the bottom, the microstrainer hugs closely the setting with breeding the groove, the microstrainer includes drainage pipe, and sets up the disinfection setting bucket through drainage pipe connection.
Furthermore, the central control system also comprises a plurality of water pumps.
Furthermore, a rotary mesh for filtering and collecting is arranged in the collecting assembly of the rotifers, and the aperture of the mesh is 80-100 micrometers.
The utility model has the following outstanding advantages:
this device easy operation, it is energy-conserving high-efficient, the culture condition is stable, but continuous cycle is cultivateed, indoor cultivation manual control temperature and environment artificial light source can be invisible external environment and continuously cultivate the target, adopt the raw materials to simply cultivate whole flow and do not need too many extra input, supply an utensil including rotifer bait is complete, this scheme improves the dissolved oxygen of culture solution and promotes the large amount of formation of dormancy ovum, survival rate and cultivation efficiency have been improved, recycle the recovery of culture solution, the cycle rate of utilization of resource has been improved, cultivate little algae for high density through the carbon dioxide generator and provide the basic condition, little algae of high density and oxygenerator provide the pure oxygen and cultivate for the rotifer of high density again and provide the basic condition, the utility model discloses the device can realize that high density, high efficiency, high standard's rotifer is cultivated.
Drawings
Fig. 1 is a schematic view of the structure of the device of the present invention.
Reference numerals: 1. a central control system; 2. a culture tank; 3. a flow meter; 4. a microalgae cultivation barrel; 5. a gas distributor; 6. a water outlet A; 7. an outlet conduit; 8. sterilizing the precipitation tank; 9. a micro-filter; 91. a water outlet B; 92. a water discharge pipeline; 10. a nano-aerosol tube; 11. a liquid level regulating tube; 12. an air lift mechanism; 13. a water inlet; 14. a carbon dioxide generator; 15. a rotifer collecting assembly; 16. an oxygen generator; 18. an air inlet.
Detailed Description
The following detailed description of the embodiments of the present invention is provided for the purpose of explanation, and should not be construed as limiting the present invention, and the embodiments do not include specific technical matters or conditions, and are performed according to the technical matters or conditions described in the literature in the field or according to the product specification, and the reagents or apparatuses are not specified, and are all conventional products available on the market.
The culture device of high-density rotifers as shown in fig. 1 is characterized by comprising a central control system 1, a microalgae culture barrel 4 and a culture tank 2, wherein the microalgae culture barrel 4 and the culture tank are controlled by the central control system 1;
the central control system 1 comprises a plurality of sensors arranged in the microalgae cultivation barrel 4 and the cultivation tank 2, an oxygen generator 16 for producing pure oxygen and a carbon dioxide generator 14 for producing carbon dioxide, wherein the oxygen generator 16 and the carbon dioxide generator 14 are respectively connected with the nano-scale gas-rock pipes 10 arranged in the cultivation tank 2 and the microalgae cultivation barrel 4;
the microalgae cultivation barrel 4 comprises a constant temperature device and a salinity regulator which are connected with the central control system 1, an outlet pipeline 7 with a flow meter is arranged at the bottom of the microalgae cultivation barrel 4, an air lifting mechanism 12 is further arranged on the microalgae cultivation barrel 4, an air distributor 5 extending into the bottom of the microalgae cultivation barrel 4 is arranged on the air lifting mechanism 12, and the outlet pipeline 7 is further connected with a water inlet A13 of the cultivation tank 2;
breed groove 2 including constant temperature equipment, salinity regulator, the liquid level control pipe 11 be connected with central control system 1, and breed and be provided with the collection subassembly 15 of rotifer in the groove 2, breed groove 2 still including setting up delivery port 91, the delivery port 91 of bottom just to the microstrainer 9 that sets up, microstrainer 9 hugs closely the setting with breeding groove 2, microstrainer 9 includes drainage pipe 92, and connects through drainage pipe 92 and set up disinfection sedimentation tank 8.
Further, the central control system 1 further comprises a plurality of water pumps.
Further, a rotary mesh for filtering and collecting is arranged in the rotifer collecting assembly 15, and the aperture of the mesh is 80-100 micrometers.
Example 1: indoor rotifer culture based on the device comprises the following steps:
the method comprises the following steps: pretreating a rotifer culture device: cleaning the culture device with clear water, placing the culture device under outdoor sun condition for insolation for 2d, taking back to the heat preservation greenhouse, standing for 1d, and cooling;
step two: culturing microalgae: introducing a microalgae culture solution into a microalgae culture barrel through a water inlet, inoculating the chlorella which is cultured and selected in advance and has vigorous vitality and no pollution, setting parameters of indoor culture microalgae through a central control system, namely starting a carbon dioxide generator for 0.5h every 3h to provide basic conditions for photosynthesis of the microalgae, starting an air lifting mechanism for 1h every 2.5h, lifting water flow through the air lifting mechanism to realize that the chlorella with a sunk bottom floats to the upper layer along with water, keeping the illumination intensity of 3000 plus 8000lx at a distance of 10-15cm from the microalgae culture barrel by a 40 watt energy-saving lamp, and simultaneously keeping the temperature in the microalgae culture barrel between 23 ℃ and 27 ℃ and regulating and controlling the temperature which exceeds the set temperature by 1 ℃;
step three: culturing rotifers: placing the pre-cultured rotifer in culture tank, inoculating at density of 1-2 g/ml, and introducing into the culture medium of step two to obtain rotifer with density not less than 3X107Adjusting water temperature to 10 ℃ and keeping the fluctuation of the water temperature not more than 2 ℃, operating a central control system to control to start an oxygen generator, filling pure oxygen into a culture tank through nano-air stones to provide a high-oxygen environment for rotifer growth, starting for 3-5 times every day for 0.5h, setting the central control system according to the rotifer growth condition, extracting microalgae liquid from a microalgae culture barrel every day after oxygenation, keeping the flow of water in the culture tank through a circulating water pump, controlling a liquid level adjusting pipe to keep the water level in the culture tank, and culturingThe culture period is 10d-20d according to the density of the rotifer, and the rotifer can be harvested when the target density is reached;
step four: collecting rotifers: the rotifer is collected for use when the density of the culture tank reaches more than 250/ml, rough density estimation can be carried out by adopting sampling visual observation or sampling is carried out through water quality analysis, the target density is achieved, a central control system control device is used for driving a salinity regulator to reduce the salinity, the rotifer is stimulated to promote a large amount of dormant eggs to be produced, a motor can be directly driven to work, the rotifer is rotationally filtered and harvested by using a 80-100-mesh bolting silk and the dormant eggs through a collecting assembly of the rotifer arranged at the bottom, and water in the culture tank is filtered and led out to a disinfection sedimentation barrel through a micro-filter;
step five: circulating cultivation: and (3) filtering the culture solution in the culture operation by using a microfiltration machine, standing and precipitating for 1 day, then culturing rotifers for the second time, introducing the treated culture solution into a culture tank, adding the dormant eggs collected in the step four according to the operation in the step three, stimulating the step three and the step four of the dormant egg incubation circulation, wherein the same culture solution is required to be replaced by new culture solution for 3 to 4 times at most, part of new culture solution is continuously added through a water inlet during use as supplement, and the replacement amount of the culture solution is generally 15 to 25 percent under the specific condition.
Preferably, the culture solution of the microalgae can be an F/2 formula culture medium solution.
Preferably, the central control system mainly controls the water temperature, the salinity, the oxygen content and the water level standard, and controls the circulating water pump to keep the freshness of water.
The high-density rotifer cultured by the scheme is more suitable for being used as bait for fry, so that the nutrition supply of the fry is improved, and the survival rate of the fry is improved.
The above description is only for the preferred embodiment of the present invention, but it should be understood that the above description should not be considered as limiting the present invention, and any modifications, equivalent substitutions, improvements, etc. made within the design concept of the present invention should be included within the protection scope of the present invention.

Claims (3)

1. A culture device for high-density rotifers is characterized by comprising a central control system (1), a microalgae culture barrel (4) and a culture tank (2), wherein the microalgae culture barrel and the culture tank are controlled by the central control system (1);
the central control system (1) comprises a plurality of sensors arranged in the microalgae cultivation barrel (4) and the cultivation tank (2), and also comprises an oxygen generator (16) for producing pure oxygen and a carbon dioxide generator (14) for producing carbon dioxide, wherein the oxygen generator (16) and the carbon dioxide generator (14) are respectively connected with the nano-aerogel pipes (10) arranged in the cultivation tank (2) and the microalgae cultivation barrel (4);
the microalgae cultivation barrel (4) comprises a constant temperature device and a salinity regulator which are connected with the central control system (1), an outlet pipeline (7) with a flowmeter is arranged at the bottom of the microalgae cultivation barrel, the microalgae cultivation barrel (4) is also provided with an air-lift mechanism (12), the air-lift mechanism (12) is provided with a gas distributor (5) which extends into the bottom of the microalgae cultivation barrel (4), and the outlet pipeline (7) is also connected with a water inlet A (13) of the cultivation tank (2);
breed groove (2) including constant temperature equipment, salinity regulator, liquid level control pipe (11) be connected with central control system (1), and breed and be provided with collection subassembly (15) of rotifer in groove (2), breed groove (2) still including setting up delivery port (91), delivery port (91) in the bottom just to microfilter (9) of setting up, microfilter (9) hug closely the setting with breeding groove (2), microfilter (9) include drainage pipe (92), and connect through drainage pipe (92) and set up disinfection sedimentation tank (8).
2. The culture device of high-density rotifers according to claim 1, characterized in that the central control system (1) further comprises a plurality of water pumps.
3. A culture apparatus for high density rotifer according to claim 1, wherein the collection assembly (15) of rotifer is provided with a rotary mesh for filtration and collection, the mesh size is 80-100 μm.
CN202020418737.6U 2020-03-27 2020-03-27 Culture apparatus of high density rotifer Active CN211932151U (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111248139A (en) * 2020-03-27 2020-06-09 浙江省海洋水产养殖研究所 Culture device and method for high-density rotifers

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111248139A (en) * 2020-03-27 2020-06-09 浙江省海洋水产养殖研究所 Culture device and method for high-density rotifers

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