CN104206359A - Method using composite bait of microalgae and other microorganisms to culture artemia - Google Patents
Method using composite bait of microalgae and other microorganisms to culture artemia Download PDFInfo
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- CN104206359A CN104206359A CN201410413004.2A CN201410413004A CN104206359A CN 104206359 A CN104206359 A CN 104206359A CN 201410413004 A CN201410413004 A CN 201410413004A CN 104206359 A CN104206359 A CN 104206359A
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- 244000005700 microbiome Species 0.000 title claims abstract description 31
- 239000002131 composite material Substances 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 21
- 241001247197 Cephalocarida Species 0.000 title claims abstract 18
- 230000012447 hatching Effects 0.000 claims abstract description 25
- 241000238582 Artemia Species 0.000 claims description 44
- 229910052736 halogen Inorganic materials 0.000 claims description 40
- 150000002367 halogens Chemical class 0.000 claims description 40
- 238000011081 inoculation Methods 0.000 claims description 22
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 239000012531 culture fluid Substances 0.000 claims description 14
- 150000003839 salts Chemical group 0.000 claims description 14
- 241000195493 Cryptophyta Species 0.000 claims description 12
- 238000002360 preparation method Methods 0.000 claims description 10
- 238000005273 aeration Methods 0.000 claims description 8
- 241000894006 Bacteria Species 0.000 claims description 6
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 6
- 241000168517 Haematococcus lacustris Species 0.000 claims description 5
- 238000005286 illumination Methods 0.000 claims description 5
- 239000013589 supplement Substances 0.000 claims description 5
- 241000206751 Chrysophyceae Species 0.000 claims description 4
- 230000000243 photosynthetic effect Effects 0.000 claims description 4
- 244000063299 Bacillus subtilis Species 0.000 claims description 3
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 3
- 240000002900 Arthrospira platensis Species 0.000 claims description 2
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 2
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 2
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 229940082787 spirulina Drugs 0.000 claims description 2
- 235000013305 food Nutrition 0.000 abstract description 3
- 238000012258 culturing Methods 0.000 abstract 6
- 241000133262 Nauplius Species 0.000 abstract 4
- 235000015097 nutrients Nutrition 0.000 abstract 1
- 239000002574 poison Substances 0.000 abstract 1
- 231100000614 poison Toxicity 0.000 abstract 1
- 230000001737 promoting effect Effects 0.000 abstract 1
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 12
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 239000013078 crystal Substances 0.000 description 6
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 6
- 235000017557 sodium bicarbonate Nutrition 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 5
- 239000000047 product Substances 0.000 description 4
- 235000013601 eggs Nutrition 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 238000011534 incubation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000006213 oxygenation reaction Methods 0.000 description 3
- 239000013535 sea water Substances 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- 239000002893 slag Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 241000894007 species Species 0.000 description 2
- 241000238569 Artemia sp. Species 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- 241001131796 Botaurus stellaris Species 0.000 description 1
- 241000287828 Gallus gallus Species 0.000 description 1
- 241000238631 Hexapoda Species 0.000 description 1
- 108090000723 Insulin-Like Growth Factor I Proteins 0.000 description 1
- 241000595940 Notostraca Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 102000013275 Somatomedins Human genes 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000013330 chicken meat Nutrition 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- MNNHAPBLZZVQHP-UHFFFAOYSA-N diammonium hydrogen phosphate Chemical compound [NH4+].[NH4+].OP([O-])([O-])=O MNNHAPBLZZVQHP-UHFFFAOYSA-N 0.000 description 1
- 229910000388 diammonium phosphate Inorganic materials 0.000 description 1
- 235000019838 diammonium phosphate Nutrition 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 235000008935 nutritious Nutrition 0.000 description 1
- 239000004465 oilseed meal Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
Landscapes
- Farming Of Fish And Shellfish (AREA)
- Feed For Specific Animals (AREA)
- Fodder In General (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a method using composite bait of microalgae and other microorganisms to culture artemia. The method comprises the following steps of (1) preparing composite bait of microalgae and other microorganisms; (2) hatching nauplius of the artemia, and separating to obtain the primarily hatched nauplius of the artemia after hatching for 24 hours; (3) inoculating and culturing the primarily hatched nauplius of the artemia, wherein an artemia culturing solution is inoculated with the microalgae; (4) culturing the artemia, feeding other microorganisms for the first time after the primarily hatched nauplius of the artemia is inoculated for 24 hours, and maintaining the concentration of the microalgae all the time. The method has the advantages that the culturing is realized by a one-step food chain type, rich nutrients are provided, the culturing is convenient, the poison and harmfulness are avoided, and the cost is very low; the composite bait of microalgae and other microorganisms contains various types of growth promoting factors, so the resistant capability of the artemia is improved, the growth is quick, and the culturing density reaches 1000-10,000ind/L.
Description
Technical field
The present invention relates to artemia culture field, especially relate to a kind of method utilizing micro-algae and other microorganism complex culture halogen worms.
Background technology
Halogen worm (Artemia sp.), also known as fairy shrimp, is a kind of a kind of microcrustacean of wide temperature salt resistance, can lives in fresh water and salt water.Halogen worm contains abundant nutriment, and dry product contains the growth factor of protein 60%, fat 20% and abundant higher unsaturated fatty acid, trace element and abundant species.Halogen worm, as a kind of living body bait of high-quality, is applied widely in aquaculture.But at present, artemia resource is limited in the world, and adult ' Luchung ' insect resource mainly relies on wild breeding, and the technology content propagating halogen worm in the world artificially is also lower, and mainly the extensive cultivation of the country such as Vietnam is main.Cultural method conventional is at present the bittern enriched based on rich water and supplementary biological feed at the initial stage of cultivation, transparency is made to remain on about 30cm, along with growing of halogen worm, need progressively to throw something and feed organic debris, as bran, oilseed meals, corn, chicken manure, cattle and sheep excrement etc.Particle is less than 50 μm, and feeding volume 30-50kg/ days hectare, gradation to feed.Have ready conditions and can use inorganic fertilizer, the per hectare water surface applies urea 20kg, diammonium hydrogen phosphate 25kg weekly.Therefore, the halogen worm high-density breeding technology of high technology content is urgently to be resolved hurrily.
Summary of the invention
The problem to be solved in the present invention is a kind of method utilizing micro-algae and other microorganism composite bait artemia cultures, realizes a step food chain high-density breeding halogen worm.
For solving the problems of the technologies described above, the technical solution used in the present invention is: a kind of method utilizing micro-algae and other microorganism composite bait artemia cultures, comprises following step,
1), the preparation of micro-algae and other microorganism composite baits;
2), the hatching of artemia nauplii, after hatching 24h, be separated and obtain Artemia nauplii;
3), the inoculation of Artemia nauplii and cultivation, wherein, in halogen worm culture fluid, inoculation has micro-algae;
4), the cultivation of halogen worm, start other microorganisms of throwing something and feeding of the first round after Artemia nauplii inoculation 24h, and remain the concentration of micro-algae.
Preferably, step 1) in, described micro-algae is salt algae, chlorella, split one in kettle algae, spirulina, chrysophyceae, haematococcus pluvialis or two or more; Other microorganisms described are yeast or photosynthetic bacteria or both mixtures.
Preferably, step 2) in, the condition of artemia nauplii hatching is that artemia hatching solution salinity is 10 ~ 30 ‰, pH is 7 ~ 9, and intensity of illumination is 1500 ~ 3000lux, after continuing inflation hatching 24h, is separated and obtains Artemia nauplii.
Preferably, step 3) in, inoculation and the cultivation of Artemia nauplii comprise the steps:
(a), preparation halogen worm culture fluid, halogen worm culture fluid salinity is 30 ~ 150 ‰, pH is 7 ~ 8, and temperature is 25 ~ 30 DEG C, and DO is 4 ~ 10ppm, and ammonia-nitrogen content is lower than 5ppm, for subsequent use after abundant aeration 24h;
B the salt algae of (), inoculation fresh cultured, makes the transparency of water body at 15 ~ 25cm.
C (), Artemia nauplii are inoculated with the density of 3 ~ 10ind/ml.
Preferably, in described step (4), in halogen worm culture fluid, the concentration basic controlling of other microorganisms is 0.5 ~ 1.5 × 10
8ind/ml, and supplement salt algae to maintain the transparency of 15 ~ 25cm.
The measuring method of artemia nauplii density that the present invention adopts is get 100 μ l halogen worm 5 times from the artemia hatching solution of mixing, counts respectively under anatomical lens, calculates five mean value density as artemia nauplii; The measuring method of the halogen polypide length adopted is Stochastic choice halogen worm 10, and under microscope mirror, measure body respectively long, the mean value calculating 10 data is long as the average body of halogen worm.
The advantage that the present invention has and good effect are: the present invention adopts micro-algae and other other microorganism composite baits as unique bait of halogen worm, adopts a step food chain mode to cultivate, nutritious, cultivation is convenient, nontoxic, cost is extremely low.And micro-algae contains the somatomedin of abundant species with other other microorganism composite baits, can improve halogen worm resistance, growth rapidly, can be adult in about 15d length, the long >1cm of body, and cultivation density can reach 1000 ~ 10,000ind/L.The halogen worm that this method is turned out is balanced in nutrition abundant, and containing rich in protein and fatty acid, resistance is high.
Embodiment
In order to understand the present invention, below by instantiation, the present invention is further described, but does not limit protection scope of the present invention.
Embodiment one
1), the preparation of micro-algae and other microorganism composite baits;
The salt algae of artificial cultivation, salinity is 80 ‰, becomes to make a critical appraisal of liquid concentration higher than 5 × 10
7ind/ml.Other microorganisms in composite bait are salt tolerant yeast.
2), the hatching of artemia nauplii;
Artemia hatching solution salinity is 30 ‰, uses the preparation of artificial sea crystal mediation running water, for subsequent use after aeration 24h.
Hatching cylinder tool 3000lux intensity of illumination, uses heating rod temperature control 28 DEG C, uses sodium bicarbonate to regulate pH to be 8.5.
The small sea strain halogen worm Product eggs that slag oxygenation provides for Tianjin Haiyou Jiayin Biology Co., Ltd., incubation rate is more than 85%.
After hatching 24h, standing separation, obtains Artemia nauplii.
3), the inoculation of Artemia nauplii and cultivation;
Halogen worm culture fluid uses sea crystal and running water to prepare, and salinity is 80 ‰, for subsequent use after abundant aeration 24h.
The salt algae of inoculation fresh cultured, makes the transparency of water body at about 20cm
Inoculation just incubates artemia nauplii, and density is 5ind/ml.
4), the cultivation of halogen worm;
Start throwing something and feeding of the first round after Artemia nauplii inoculation 24h, in halogen worm culture fluid, the concentration basic controlling of yeast is 1 × 10
8ind/ml.Throw something and feed every day 3 times, and suitably supplement salt algae to maintain the transparency of 20cm.
In breeding process, controlling water temperature is 28 ~ 30 DEG C, and pH uses sodium bicarbonate solution to regulate 5 to control between 7 ~ 8, and DO remains on more than 3ppm.
(5), when cultivating 20d, artemia density maintains about 3ind/ml.Use 120 mesh filter screens to collect halogen worm polypide, drain after using clean sea water to rinse, weight in wet base can reach 18.2kg/m3.
Embodiment two
1), the preparation of micro-algae and other microorganism composite baits;
Described micro-algae is the artificial haematococcus pluvialis cultivated, and salinity is 100 ‰, becomes to make a critical appraisal of liquid concentration higher than 5 × 10
7ind/ml.Other microorganisms in composite bait are bacillus subtilis.
2), the hatching of artemia nauplii;
Artemia hatching solution salinity is 30 ‰, uses the preparation of artificial sea crystal mediation running water, for subsequent use after aeration 24h.
Hatching cylinder tool 3000lux intensity of illumination, uses heating rod temperature control 28 DEG C, uses sodium bicarbonate to regulate pH to be 8.5.
The Tibet strain halogen worm Product eggs that slag oxygenation provides for Tianjin Haiyou Jiayin Biology Co., Ltd., incubation rate is more than 85%.
After hatching 24h, standing separation, obtains Artemia nauplii;
3), the inoculation of Artemia nauplii and cultivation;
Halogen worm culture fluid uses sea crystal and running water to prepare, and salinity is 100 ‰, for subsequent use after abundant aeration 24h.
The haematococcus pluvialis of inoculation fresh cultured, makes the transparency of water body at about 20cm
Inoculation just incubates artemia nauplii, and density is 5ind/ml.
4), the cultivation of halogen worm;
Start throwing something and feeding of the first round after Artemia nauplii inoculation 24h, in halogen worm culture fluid, the concentration basic controlling of bacillus subtilis is 1 × 10
8ind/ml.Throw something and feed every day 3 times, and suitably supplement haematococcus pluvialis to maintain the transparency of 20cm.
In breeding process, controlling water temperature is 28 ~ 30 DEG C, and pH uses sodium bicarbonate solution to regulate 5 to control between 7 ~ 8, and DO remains on more than 3ppm.
(5), when cultivating 18d, artemia density maintains about 8ind/ml.Use 120 mesh filter screens to collect halogen worm polypide, drain after using clean sea water to rinse, weight in wet base can reach 26kg/m
3.
Embodiment three
1), the preparation of micro-algae and other microorganism composite baits;
Described micro-algae is the artificial chrysophyceae cultivated, and salinity is 150 ‰, becomes to make a critical appraisal of liquid concentration higher than 5 × 10
7ind/ml.Other microorganisms in composite bait are photosynthetic bacteria.
2), the hatching of artemia nauplii;
Artemia hatching solution salinity is 30 ‰, uses the preparation of artificial sea crystal mediation running water, for subsequent use after aeration 24h.
Hatching cylinder tool 3000lux intensity of illumination, uses heating rod temperature control 28 DEG C, uses sodium bicarbonate to regulate pH to be 8.5.
The Tibet strain halogen worm Product eggs that slag oxygenation provides for Tianjin Haiyou Jiayin Biology Co., Ltd., incubation rate is more than 85%.
After hatching 24h, standing separation, obtains Artemia nauplii.；
3), the inoculation of Artemia nauplii and cultivation;
Halogen worm culture fluid uses sea crystal and running water to prepare, and salinity is 150 ‰, for subsequent use after abundant aeration 24h.
The chrysophyceae of inoculation fresh cultured, makes the transparency of water body at about 20cm
Inoculation just incubates artemia nauplii, and density is 5ind/ml.
4), the cultivation of halogen worm;
Start throwing something and feeding of the first round after Artemia nauplii inoculation 24h, in halogen worm culture fluid, the concentration basic controlling of photosynthetic bacteria is 1 × 10
8about ind/ml.Throw something and feed every day 3 times, and suitably supplement salt algae to maintain the transparency of about 20cm.
In breeding process, controlling water temperature is 28 ~ 30 DEG C, and pH uses sodium bicarbonate solution to regulate 5 to control between 7 ~ 8, and DO remains on more than 3ppm.
(5), when cultivating 16d, artemia density maintains about 7ind/ml.Use 120 mesh filter screens to collect halogen worm polypide, drain after using clean sea water to rinse, weight in wet base can reach 24.5kg/m
3.
Above embodiments of the invention have been described in detail, but described content being only the preferred embodiment of the invention, can not being considered to for limiting practical range of the present invention.All equalizations done according to the invention scope change and improve, and all should still belong within this patent covering scope.
Claims (6)
1. utilize a method for micro-algae and other microorganism composite bait artemia cultures, it is characterized in that: comprise following step,
1), the preparation of micro-algae and other microorganism composite baits;
2), the hatching of artemia nauplii, after hatching 24h, be separated and obtain Artemia nauplii;
3), the inoculation of Artemia nauplii and cultivation, wherein, in halogen worm culture fluid, inoculation has micro-algae;
4), the cultivation of halogen worm, start other microorganisms of throwing something and feeding of the first round after Artemia nauplii inoculation 24h, and remain the concentration of micro-algae.
2. the method utilizing micro-algae and other microorganism composite bait artemia cultures according to claim 1, it is characterized in that: step 1) in, described micro-algae is salt algae, chlorella, split one in kettle algae, spirulina, chrysophyceae, haematococcus pluvialis or two or more; Other microorganisms described are yeast or bacterium or both mixtures.
3. the method utilizing micro-algae and other microorganism composite bait artemia cultures according to claim 2, is characterized in that: described yeast is salt tolerant yeast; Described bacterium is bacillus subtilis or photosynthetic bacteria.
4. the method utilizing micro-algae and other microorganism composite bait artemia cultures according to claim 3, it is characterized in that: step 2) in, the condition of artemia nauplii hatching is, artemia hatching solution salinity is 10 ~ 30 ‰, pH is 7 ~ 9, intensity of illumination is 1500 ~ 3000lux, after continuing inflation hatching 24h, is separated and obtains Artemia nauplii.
5. the method utilizing micro-algae and other microorganism composite bait artemia cultures according to any one of Claims 1 to 4, is characterized in that: step 3) in, inoculation and the cultivation of Artemia nauplii comprise the steps:
(a), preparation halogen worm culture fluid, halogen worm culture fluid salinity is 30 ~ 150 ‰, pH is 7 ~ 8, and temperature is 25 ~ 30 DEG C, and DO is 4 ~ 10ppm, and ammonia-nitrogen content is lower than 5ppm, for subsequent use after abundant aeration 24h;
B the salt algae of (), inoculation fresh cultured, makes the transparency of water body at 15 ~ 25cm.
C (), Artemia nauplii are inoculated with the density of 3 ~ 10ind/ml.
6. the method utilizing micro-algae and other microorganism composite bait artemia cultures according to claim 1, it is characterized in that: in described step (4), in halogen worm culture fluid, the concentration basic controlling of other microorganisms is 0.5 ~ 1.5 × 10
8ind/ml, and supplement salt algae to maintain the transparency of 15 ~ 25cm.
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| CN201410413004.2A CN104206359B (en) | 2014-08-20 | 2014-08-20 | A kind of method of utilizing micro-algae and other microorganism composite bait artemia cultures |
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766810A (en) * | 2016-04-06 | 2016-07-20 | 鲁东大学 | Method for indoor low-cost high-density sustainable cultivation of artemia |
| CN106165790A (en) * | 2016-08-31 | 2016-11-30 | 天津海友佳音生物科技股份有限公司 | A kind of bait for artemia culture and artemia culture method |
| CN107494343A (en) * | 2017-07-31 | 2017-12-22 | 兰溪市普润斯水产养殖技术有限公司 | A kind of artificial fecundation method of loach |
| CN109744404A (en) * | 2017-11-06 | 2019-05-14 | 南风化工集团股份有限公司 | Agreeable to the taste feed of a kind of ecology artemia and preparation method thereof |
| CN110214730A (en) * | 2019-06-28 | 2019-09-10 | 中国科学院青岛生物能源与过程研究所 | The artemia and its acclimation method of fast breeding under high temperature and high salt environment |
| CN110754405A (en) * | 2019-11-29 | 2020-02-07 | 广东海洋大学 | Large-scale production method of artemia salina imago |
| CN110856526A (en) * | 2018-08-22 | 2020-03-03 | 南风化工集团股份有限公司 | Brine shrimp feed for salt pan and preparation method thereof |
| WO2020190222A1 (en) * | 2019-03-19 | 2020-09-24 | Artkom Yem Sanayi̇ İç Ve Diş Ti̇caret Li̇mi̇ted Şi̇rketi̇ | A method which enables hatching of artemia eggs |
| CN112293606A (en) * | 2020-10-12 | 2021-02-02 | 上海海洋大学 | Method for preparing high-value aquatic bait by using microalgae and artemia |
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Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105766810A (en) * | 2016-04-06 | 2016-07-20 | 鲁东大学 | Method for indoor low-cost high-density sustainable cultivation of artemia |
| CN105766810B (en) * | 2016-04-06 | 2019-02-22 | 鲁东大学 | A kind of indoor low cost, high density, sustainable cultural method of artemia |
| CN106165790A (en) * | 2016-08-31 | 2016-11-30 | 天津海友佳音生物科技股份有限公司 | A kind of bait for artemia culture and artemia culture method |
| CN107494343A (en) * | 2017-07-31 | 2017-12-22 | 兰溪市普润斯水产养殖技术有限公司 | A kind of artificial fecundation method of loach |
| CN109744404A (en) * | 2017-11-06 | 2019-05-14 | 南风化工集团股份有限公司 | Agreeable to the taste feed of a kind of ecology artemia and preparation method thereof |
| CN110856526A (en) * | 2018-08-22 | 2020-03-03 | 南风化工集团股份有限公司 | Brine shrimp feed for salt pan and preparation method thereof |
| WO2020190222A1 (en) * | 2019-03-19 | 2020-09-24 | Artkom Yem Sanayi̇ İç Ve Diş Ti̇caret Li̇mi̇ted Şi̇rketi̇ | A method which enables hatching of artemia eggs |
| CN110214730A (en) * | 2019-06-28 | 2019-09-10 | 中国科学院青岛生物能源与过程研究所 | The artemia and its acclimation method of fast breeding under high temperature and high salt environment |
| CN110754405A (en) * | 2019-11-29 | 2020-02-07 | 广东海洋大学 | Large-scale production method of artemia salina imago |
| CN110754405B (en) * | 2019-11-29 | 2021-11-23 | 广东海洋大学 | Large-scale production method of artemia salina imago |
| CN112293606A (en) * | 2020-10-12 | 2021-02-02 | 上海海洋大学 | Method for preparing high-value aquatic bait by using microalgae and artemia |
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