CN201442951U - Airlift type photo-bioreactor - Google Patents

Airlift type photo-bioreactor Download PDF

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Publication number
CN201442951U
CN201442951U CN 200920029774 CN200920029774U CN201442951U CN 201442951 U CN201442951 U CN 201442951U CN 200920029774 CN200920029774 CN 200920029774 CN 200920029774 U CN200920029774 U CN 200920029774U CN 201442951 U CN201442951 U CN 201442951U
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China
Prior art keywords
tank body
bioreactor
sealing cover
reactor tank
passage
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Expired - Fee Related
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CN 200920029774
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Chinese (zh)
Inventor
吴怀之
吴美玲
田亮
李森茂
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Qingdao Institute of Bioenergy and Bioprocess Technology of CAS
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Qingdao Institute of Bioenergy and Bioprocess Technology of CAS
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Priority to CN 200920029774 priority Critical patent/CN201442951U/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00—Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02—Photobioreactors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/18—Flow directing inserts
    • C12M27/24—Draft tube
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C12M29/08—Air lift
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00—Means for providing, directing, scattering or concentrating light
    • C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
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  • Clinical Laboratory Science (AREA)
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Abstract

The utility model discloses an airlift type photo-bioreactor, comprising an internal light source device in a tank body of the bioreactor, an inoculation opening and a discharging opening on the wall of the tank body of the bioreactor, a measuring opening and an air exhaust opening on the top of the tank body of the bioreactor, as well as a lower sealing cover and a temperature controlling device at the bottom of the tank body of the bioreactor. The airlift type photo-bioreactor is characterized by further comprising a distributor device fixed on the central through hole of the lower sealing cover, wherein the distributor device is communicated with an inner chamber of the tank body of the bioreactor by a plurality of distributing passageways therein, and nutrient substance respectively enters the distributing passageways through a material-injecting hole from a material-charging hole. The stepped round platform of the distributor device is externally provided with a sealing cover which is mutually covered; and ring-shaped chambers on the sealing cover, corresponding to the distributing passageways, are internally provided with a one-way valve respectively. The airlift type photo-bioreactor realizes uniform distribution and mixing of multi-media in the bioreactor, can prevent culture medium from inversely flowing into the distributing passageways, is reasonable in structure, convenient and reliable for injecting the nutrient substance, and applicable to laboratory or industrial large-scale microalgae cultivation and the suspension cultivation of other photosynthetic biological cells, and has better industry scale-up application prospect.

Description

A kind of airlift photobioreactor
Technical field
The invention belongs to little algae sealing breed bioreactor---a kind of airlift photobioreactor.
Background technology
Algae belongs to microphyte, and little algae can utilize simple mineral nutrient, under the situation of photosynthesis (daylight and artificial lighting), with CO 2Be converted into the various organic compound (as the nutrition of needed by human such as pigment, protein, amino acid, VITAMIN and lipid acid, polysaccharide and mineral substance) of our living needs with water.Because algae has the efficiency light utilization ratio, and rapidly and efficiently absorbs nutrient substance (as CO from surrounding environment 2, nitrogen, phosphorus etc.) and change into the ability of organic compound, algae is become has the tremendous economic potentiality that do not solve food, energy dilemma.Algal culture is for CO 2The vital role of reduction of discharging, sewage disposal and clean biometric energy utilization aspect and the important meaning of improving the ecological environment, make laboratory culture and the industrialized aquaculture of algae kind improvement laboratory scientific research, little algae, and the comprehensive utilization technique of little algae popularization research, become one of at present the most popular scientific research and Application Areas.Although algae has possessed higher environment and economic worth, realize the economy utilization of algae industry, still be subject to the development of the bioreactor of industrial scale algal culture at present.
Current, the cultivation of algae mainly contains open and closed two kinds of bioreactors, open type biological reactor is not owing to possess temperature control capability, produce and to be subjected to seasonal effect, and production process is subjected to the pollution of various heteroplasia things easily and proves not to be efficient manner.For this reason, people's various closed photo bioreactors that begin one's study are as bioreactors such as duct type, flat, column air lift type, floating bag films.Because these bioreactor structure differences, its practical effect is widely different, and product structure is just more obvious with the influence of culture efficiency to the cultured output of algae.
Existing notification number CN2384948Y, and Kang Ruijuan etc. is published in the paper of " airlift photobioreactor that is used for little algae cultivation " on " chemical reaction engineering and technology 2002,17 (1) ".Public technology make up based on circulation bioreactor in the inside and outside light source bonded air lift type, this product mainly partly is made up of tank body, gas-lift tube, inner light source sealed tube, heat exchanger, gas distributor, inside and outside light source etc.Its core technology is: adopt fluorescent tube as inside and outside light source; Adopt gas distributor to make the mixed gas of feeding form tiny bubble, improve the mass transfer area and the effect of gas-liquid; Adopt heat exchanger heating or cooling culture-liquid temp.Notification number CN2748464Y has only done necessary modifications at the stability in use and the security of its structural part on last patented technology basis.The problem of technical main existence is that the inner light source sealed tube adopts one section or the manufacturing of two sections bulge loop glassworks, adds the structure formation of O shape seal with elastometic washer again, sealed tube processing and manufacturing and assembling process complexity, cost height; Adopt the structure of gas distributor to play the distribution effect to this a kind of injected media of gas, and can't prevent that nutrient solution from blowing back into sparger, in the gas distributor use, distributor orifices is subjected to little algae and grows blockedly easily, influences the life-time service effect; In addition, adopt the way that in tank body, installs heat exchanger additional, also can be dead owing to heater table surface temperature height comes to harm the part culture, influence output; Moreover, because its internal support member is more, be unfavorable for mixing of materials, cause little algae to adhere to the influence growth, and can shut out the light penetration power and influence overall photosynthesis effect.
Summary of the invention
The purpose of this invention is to provide a kind of airlift photobioreactor, to overcome the many deficiencies on prior art structure and the life-time service performance thereof.
The present invention is the improvement to existing column airlift photobioreactor structure and function, by adopting plain dispenser device of a kind of novel nourishing and temperature control mode, make the interior material feeding uniform distribution of reactor tank body and do not have obstruction, temperature controller is arranged on the external temperature of carrying out of reactor tank and controls, realized higher, the more rational processing of stability to multimedium nutrient substance (as nutrient solution etc.), littler to the damage of culture in the tank body.
Technical scheme of the present invention comprises the intravital inner light source device of reactor tank, inoculation mouth and discharge gate on the reactor tank body wall, and the following capping and the temperature controller of gauge hatch, venting port and bottom in the capping at reactor tank body top, it is characterized in that also comprising the dispenser device that is fixed on down on the capping central through hole, and communicate with the inner cavity chamber of reactor tank body via a plurality of distribution passages wherein, feeding tube enters respectively via material hole and distributes in the passage.
Dispenser device is the ladder truncated cone-shaped, and each distributes the outlet of passage to be arranged on frustum cone side or the top.
Consider that culture blows back into the possible situation of distributing passage to stop up the distribution passage, there is a sealing cover that covers mutually the ladder truncated cone-shaped outside of described dispenser device, the correspondence of sealing cover on it respectively distributed in the annular chamber surrounding of passage and is provided with check valve, isolated by O-ring seals between annular chamber surrounding.
Dispenser device is made up of ladder round platform and corresponding sealing cover, check valve, O-ring seals, after distribution passage and sealing cover are equipped, forms several annular chamber surroundings at the ladder place, is isolated by O-ring seals between annular chamber surrounding; On the side of sealing cover or end face check valve is installed, check valve can allow nutrient substance to inject and prevent that nutrient solution from blowing back into the distribution passage; Wherein material hole distributes passage to be communicated with annular chamber surrounding, and is T-shaped or L shaped, and dispenser device and sealing cover are pressed on the reactor tank body by following capping.
Temperature controller mainly is arranged on the external temperature of carrying out of reactor tank and controls.Temperature when temperature controller injects by heating or cooling nutrient substance, the temperature of control substratum fluctuates in the scope of setting.When the temperature of substratum was higher than the temperature value of setting, temperature controller reached the purpose that reduces the substratum temperature by reducing the temperature of the nutrient substance that injects; When the substratum temperature was lower than design load, temperature controller reached the purpose of rising substratum temperature by the temperature of each nutrient substance that comprises mixed gas of heating raising injection.Since the heating and cooling process at be inorganic medium, utilize little temperature difference mixing of materials to regulate the temperature of substratum, temperature variation is soft, and is little to algae bio culturing process influence.
The present invention has realized the uniformly distributing of multimedium nutrient substance in nutrient solution and has mixed, and installs check valve additional at the mouth that distributes, and prevents that nutrient solution from blowing back into the situation of distributing passage material hole blocking channel; The inner light source seal closure processing of design is easier, dismounting and cleaning is more convenient; External temperature controller is adopted in the control of the temperature of substratum, realize that by the charge temperature of regulating inorganic nutritive element the substratum temperature changes in the temperature range of regulation, has reduced the possibility that heating rod localized hyperthermia district causes culture death.Therefore; of the present invention rational in infrastructure; it is convenient reliable with the control of substratum temperature that nutrient substance injects control; inner light source illuminance controlled range is wideer; be applicable to laboratory or industrial scale both culturing microalgae and other photosynthetic organism suspension culture of cells, have laboratory applications and commercial run amplification prospect preferably.
With reference to the accompanying drawings the preferred embodiments of the present invention are elaborated.
Description of drawings
Fig. 1 is the single bioreactor basic structure of a present invention synoptic diagram.
Wherein, 1. the reactor tank body 2. descends capping, 3. temperature controller, and 4. dispenser device, 41. distribute passages, 42. material holes, 43. annular chamber surroundings, 44.O shape sealing-ring, 45. sealing covers, 46. check valves; 5. seal closure, 6. inoculation mouthful, 7. inner light source device 71. inner light sources, 72. supports, 73. ventages, 74. light controllers, 75. insulcretes, 8. gauge hatch, 9. venting port, 10. capping, 11. discharge gates, 12. feeding tubes.
Specific embodiment
As shown in Figure 1, the present invention includes the inner light source device 7 in the reactor tank body 1, inoculation mouth 6 and discharge gate 11 on reactor tank body 1 wall, the following capping 2 of the gauge hatch 8 in the capping 10 at reactor tank body 1 top, venting port 9 and bottom and temperature controller 3, it is characterized in that also comprising the dispenser device 4 that is fixed on down on capping 2 central through holes, and via distributing passage 41 to communicate with the inner cavity chamber of reactor tank body 1, the nutrient substance in the feeding tube 12 enters via material hole 42 and distributes in the passage 41.
Described dispenser device 4 is ladder truncated cone-shaped, and each distributes the outlet of passage 41 to be arranged on stepped appearance frustum cone side or the top.
The stepped appearance round platform of described dispenser device 4 outside has a sealing cover that covers mutually 45, the correspondence on it respectively to distribute in the annular chamber surrounding 43 of passage 41 to be provided with 8 check valves 46.
Ladder truncated cone-shaped dispenser device 4 described in Fig. 1 is by distributing passage 41, O-ring seals 44, sealing cover 45 and check valve 46 to form, distribute the inlet of passage 41 that four nutrient substance material holes 42 that comprise mixed gas are arranged, form annular chamber surrounding 43 when distributing passage 41 to be equipped with sealing cover 45, isolate by O-ring seals 44 between the annular chamber surrounding 43, there are some distributions mouthful (also can at its top drilling) side of sealing cover 45, is used to install check valve 46.Distribute passage 41 to be T type or L type passage, with corresponding annular chamber surrounding 43 connections, each material hole 42 is plain as CO with the Different Nutrition of adjusting through temperature controller 3 respectively 2, nutrient substance feeding tubes 12 such as air, nitrogenous or phosphorus containg substances connect.During respectively from corresponding material hole 42 material feedings, by regulating the reinforced total amount of its feed rate control, when feed pressure greater than reactor in during substratum pressure, check valve 46 is opened, various nutrient substance homodisperse ground joins the substratum from check valve 46 wherein, and under gas bubbling situation, quicken the mixing and the diffusion of nutrient substance; When nutrient substance stopped material feeding, check valve 46 was closed, and prevented that substratum from blowing back into dispenser device 4, stopped up and distributed passage.Obviously adopt gas dispenser device 4 can strengthen the mixing of various media and the disturbance of hot housing liquid, do not stop up the distribution passage again.
Temperature controller 3 is arranged on the reactor tank body 1 outer temperature of carrying out and controls.
Reactor tank body 1 is made when light source (outside not needing, can select non-transparent material manufacturings such as pottery for use) by glass, synthetic glass or polyethylene transparent material, and the following capping 2 of middle perforate is provided with bayonet socket with capping 10 and is connected with reactor tank body 1.Following capping 2 compresses dispenser device 4 and compresses and reactor tank body 1 bottom gasket seal (omitting among the figure), guarantees the sealing installation requirement of reactor tank body 1 bottom.Perforate in the middle of the following capping 2 is fixed wherein dispenser device 4, and the sprue 42 of reactor tank body 1 bottom is connected with the plain feeding tube 12 of reactor tank body 1 external nutrition.
Described capping 10 is provided with a plurality of gauge hatch 8, is respectively applied for the measurement to data such as medium pH value, temperature, dissolved oxygen in the reactor tank body 1; Venting port 9 is used for insoluble or overflows gas purging; Perforate in the middle of the capping 10 is used for installing and fixing of seal closure 5 and inner light source device 7.
The seal closure 5 of described inner light source device 7 structure that takes the shape of the letter U is made by transparent material, and its length and internal diameter can be chosen appropriate value according to the light path of algal culture.The flanging at seal closure 5 tops and capping 10 are equipped; And can pull down the seal closure 5 that seal closure 5 is removed its surface attachments or changed other size.
Described inner light source device 7 is made up of inner light source 71, support 72, light controller 74 and the insulcrete 75 of many groups of illuminations, inner light source 71 is fixed on the support 72, support 72 is connected with insulcrete 75, the ventage 73 of being convenient to inner light source 71 heat radiations is arranged on the insulcrete 75, insulcrete 75 is connected with upper cover 10, and compress seal closure 5, can guarantee that substratum does not leak outside; Common light controller 74 can be controlled the brightness value of inner light source 71.
The present invention's one-tenth series connection capable of being combined or cultivating system in parallel are realized special cultivation and large-scale farming target.
The present invention also is not difficult by its fixed support adjustment, realizes different setting angle control requirements.

Claims (4)

1. airlift photobioreactor, comprise the inner light source device (7) in the reactor tank body (1), inoculation mouth (6) on reactor tank body (1) wall and discharge gate (11), gauge hatch (8) in the capping (10) at reactor tank body (1) top, the following capping (2) of venting port (9) and bottom and temperature controller (3), it is characterized in that also comprising the dispenser device (4) that is fixed on down on capping (2) central through hole, and via distributing passage (41) to communicate with the inner cavity chamber of reactor tank body (1), the nutrient substance in the feeding tube (12) enters via material hole (42) and respectively distributes in the passage (41).
2. airlift photobioreactor as claimed in claim 1 is characterized in that described dispenser device (4) is the ladder truncated cone-shaped, and each distributes the outlet of passage (41) to be arranged on frustum cone side or the top.
3. airlift photobioreactor as claimed in claim 1 or 2, there is a sealing cover that covers mutually (45) the ladder truncated cone-shaped outside that it is characterized in that described dispenser device (4), the correspondence of sealing cover (45) on it respectively distributed in the annular chamber surrounding (43) of passage (41) and is provided with check valve (46), isolated by O-ring seals (44) between annular chamber surrounding (43).
4. airlift photobioreactor as claimed in claim 1 or 2 is characterized in that above-mentioned temperature controller (3) is arranged on the outer temperature of carrying out of reactor tank body (1) and controls.
CN 200920029774 2009-07-16 2009-07-16 Airlift type photo-bioreactor Expired - Fee Related CN201442951U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 200920029774 CN201442951U (en) 2009-07-16 2009-07-16 Airlift type photo-bioreactor

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Application Number Priority Date Filing Date Title
CN 200920029774 CN201442951U (en) 2009-07-16 2009-07-16 Airlift type photo-bioreactor

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101899391A (en) * 2010-07-16 2010-12-01 常熟琦光光电科技有限公司 Special spectrum airlift photobioreactor
CN102533522A (en) * 2011-11-30 2012-07-04 镇江绿能环保科技有限公司 Full-plastic airtight modularized airlift light biological reactor
CN102559491A (en) * 2010-10-14 2012-07-11 杨凯 A kind of bubble bioreactor for tissue culture of lily bulbs
CN102716705A (en) * 2011-03-30 2012-10-10 中国人民解放军63975部队 Experimental facility for chemical reaction
CN108315226A (en) * 2018-03-15 2018-07-24 郭敬承 A kind of center air lift type high-efficient biological reactor
CN109266513A (en) * 2018-08-28 2019-01-25 季华实验室 Brewing apparatus, brewing system and brewing method
US20230105778A1 (en) * 2012-03-16 2023-04-06 Forelight, Inc. Methods and materials for cultivation and/or propagation of a photosynthetic organism

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101899391A (en) * 2010-07-16 2010-12-01 常熟琦光光电科技有限公司 Special spectrum airlift photobioreactor
CN102559491A (en) * 2010-10-14 2012-07-11 杨凯 A kind of bubble bioreactor for tissue culture of lily bulbs
CN102716705A (en) * 2011-03-30 2012-10-10 中国人民解放军63975部队 Experimental facility for chemical reaction
CN102716705B (en) * 2011-03-30 2015-07-15 中国人民解放军63975部队 Experimental facility for chemical reaction
CN102533522A (en) * 2011-11-30 2012-07-04 镇江绿能环保科技有限公司 Full-plastic airtight modularized airlift light biological reactor
CN102533522B (en) * 2011-11-30 2013-07-24 镇江绿能环保科技有限公司 Full-plastic airtight modularized airlift light biological reactor
US20230105778A1 (en) * 2012-03-16 2023-04-06 Forelight, Inc. Methods and materials for cultivation and/or propagation of a photosynthetic organism
CN108315226A (en) * 2018-03-15 2018-07-24 郭敬承 A kind of center air lift type high-efficient biological reactor
CN109266513A (en) * 2018-08-28 2019-01-25 季华实验室 Brewing apparatus, brewing system and brewing method

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