CN201245657Y - Photobioreactor for microalgae cultivation - Google Patents

Photobioreactor for microalgae cultivation Download PDF

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Publication number
CN201245657Y
CN201245657Y CNU2008200571065U CN200820057106U CN201245657Y CN 201245657 Y CN201245657 Y CN 201245657Y CN U2008200571065 U CNU2008200571065 U CN U2008200571065U CN 200820057106 U CN200820057106 U CN 200820057106U CN 201245657 Y CN201245657 Y CN 201245657Y
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Prior art keywords
tube
light source
biological respinse
reaction
cylinder
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Expired - Fee Related
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CNU2008200571065U
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Chinese (zh)
Inventor
王崇
孔海南
何圣兵
陈纬栋
鲁佳铭
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Shanghai Jiaotong University
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Shanghai Jiaotong University
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18Heat exchange systems, e.g. heat jackets or outer envelopes
    • C12M41/24Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/08Means for providing, directing, scattering or concentrating light by conducting or reflecting elements located inside the reactor or in its structure
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
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Abstract

The utility model provides a photo-bioreactor for culturing microalgae. The photo-bioreactor comprises a light source, a light source divergence cylinder, a biological reaction cylinder, an emptying bucket, pillars, a supporting chassis. The light source is arranged at the top end of a conical cylindrical light source divergence cylinder; the light source divergence cylinder inversely sleeved on the upper end of the cylindrical biological reaction cylinder; a spiral heating pipe is installed in the biological reaction cylinder; a temperature, alkalinity and salinity determination device is arranged in the reaction cylinder; the emptying bucket is arranged at the lower end of the reaction sleeve; an emptying tube and an aeration tube are arranged on the lower part of the emptying bucket; and three pillars used for supporting the reaction sleeve are fixed on the supporting chassis. Furthermore, specular reflective materials are coated on the inner wall or the outer wall of the reactor so as to ensure the light emitted by the light source can be uniformly reflected into the reaction sleeve, so that the light using efficiency is improved; the gas exchange in the reactor can be enhanced through the aeration so as to drive the reaction solution to be evenly mixed; and a hydrothermal heating pipe can keep the reaction temperature stable even in temperature season. Therefore, the utility model can improve the microalgae production and quality.

Description

Little algae is cultivated bioreactor
Technical field
The utility model relates to a kind of little algae and cultivates bioreactor, is used to improve output and the quality of little algae, belongs to little algae and cultivates biological technical field.
Background technology
Little algae generally is a ptotoautotroph, and growth potential is huge, is the producer in the ecosystem, is the important component part of primary productive force in the biosphere.One slightly algae be the important bait of aquaculture; Part in little algae is rich in nutritive ingredient, is human fabulous nutritious prod, as the spirulina rich in proteins; Also some little algae grows rapidly, can absorb the nutritive elements such as nitrogen phosphorus in the water body in a large number, becomes the biology of novel pollution control of water.Because little algae biological yield is big, biomass are abundant, therefore can be used for producing novel energies such as ethanol, biofuel, bio-feritlizer.
The principal element that influences micro algae growth and cellular constituent is illumination, temperature, salinity, basicity etc., and suitable culture condition is extremely important for the output and the quality that improve little algae.
Cultivation of present little algae and production process mainly adopt the cement pit open culture, and part adopts the retort of transparent sealing.Cement pit open culture mode, equipment is simple, can carry out scale operation, but since big with the air contact area, be subjected to the harmful animal biological pollution easily, influence output and quality; Simultaneously because micro algae growth is subjected to the influence of temperature, illumination easily, different seasons its production process very unstable.The training method of the glass pot of transparent sealing, because micro algae growth is rapid, cause the interior mixing of reactor inhomogeneous easily, cause effectively radiation light intensity decline in the reactor, influence the growth of little algae, even causing the death of little algae to be decomposed, the while is wayward as the temperature that influences the micro algae growth important factor, directly has influence on output and the quality of little algae.
Summary of the invention
The purpose of this utility model is the deficiency at existing little algae culture technique, proposes a kind of little algae and cultivates bioreactor, the light radiation intensity in can the conditioned reaction device, improves the utilising efficiency of light, is difficult for being subjected to the harmful animal biological pollution.
For achieving the above object, the utility model proposed, and little algae cultivates that bioreactor mainly comprises that light source, light source are dispersed tube, biological respinse tube, emptying bucket, pillar and support chassis.Light source is positioned at the topmost of whole reactor, is installed in the top that awl tubular light source is dispersed tube, and light source is dispersed the upper end that a back-off is installed in biological respinse tube cylindraceous.Disperse the middle part of tube at light source the Bright Source Protection layer is installed, disperse at the light source of Bright Source Protection layer bottom a ventpipe is installed on the barrel.The spirrillum heating tube is installed in the biological respinse tube, and the water outlet of the water-in of heating tube lower end and heating tube upper end all stretches out outside the wall of biological respinse tube; Temperature measuring device, basicity tester and salinity tester are installed in the biological respinse tube, and the control panel of above-mentioned three testers is installed in respectively on the outer wall of biological respinse tube.The lower end of biological respinse tube is connected and installed awl tubular emptying bucket, and point mouth place, emptying bucket bottom connects the blow-down pipe that valve is housed, and the emptying bucket is installed two aeration tubes that valve is housed near symmetry on the outer wall of sharp mouth; Three pillars equidistantly are supported on biological respinse tube and emptying bucket joining place, and the pillar lower end is fixed on the support chassis.
Described light source generally can adopt the cold lamp of the metal halide that has the white fluorescent coating.
Described light source is dispersed tube, biological respinse tube and emptying bucket are transparent material (as organic glass) and make, and inwall scribbles or outer wall covers the minute surface light reflecting material, guarantees to improve the utilising efficiency of light in the equal reflected back biological respinse of the light tube that light source sends.
Light source is dispersed tube and is designed to bore tubular, light source is installed in the vertex of a cone, so that the light full illumination is in the biological respinse tube.In light source is dispersed tube, be provided with one deck waterproof Bright Source Protection layer between light source and the biological respinse tube, prevent that water vapour from causing light source to damage.Can also place some shade materials on the Bright Source Protection layer, to regulate the intensity of illumination in the biological respinse tube.
The biological respinse tube is a cylinder, and its height is 1:0.8~1.2 with the ratio of cylinder section diameter, in order to avoid the intensity of illumination of lower floor is low excessively in the tube.The helical centre of spirrillum heating tube overlaps with the cylinder center line, and heating tube generally adopts stainless steel, and heating tube guarantees the growth temperature in the little algae of low temperature season by the reaction solution heat supply of hot water circulation in the biological respinse tube.Add hot water and enter from the water-in of heating tube lower end, the water outlet of upper end flows out.Medium position at the biological respinse tube is installed temperature, salinity, basicity tester, and their control panel is installed on the outer wall of reaction tube, at any time temperature, salinity and the basicity in the monitoring reaction device.When the salinity in the reactor and basicity need be regulated, can regulate by ventpipe dosing in reactor that light source is dispersed on the tube.
Can carry out aeration to reaction solution by the aeration tube of emptying bucket bottom, play the effect of strengthening gaseous interchange in the reactor, aeration can make reaction solution evenly mixed simultaneously, thereby improves light efficiency, improves little algae output and quality.Little algae is cultivated when acquiring a certain degree, and the blow-down pipe of emptying bucket bottom can be emitted reaction solution fully.
Support chassis is a plane disc, and its diameter should be more bigger than the diameter of section of biological respinse tube.They all should adopt the higher matrix material of intensity to make.
It is simple in structure that little algae that the utility model provides is cultivated bioreactor, realizes easily.Light radiation intensity in the reactor can be regulated, to improve the utilising efficiency of light; Can carry out aeration aerating, strengthen gaseous interchange, the reaction solution in the reactor is mixed.The hydro-thermal heating tube is arranged in the reactor, can be stable in the low temperature season holding temperature, help to improve output and the quality of little algae.
Description of drawings
Fig. 1 is that little algae is cultivated the bioreactor structural representation.
Among Fig. 1: 1 is light source, and 2 disperse tube for light source, and 3 is ventpipe; 4 is biological reaction tube, and 5 is temperature measuring device, and 6 is the basicity tester; 7 is pillar, and 8 is support chassis, and 9 is the Bright Source Protection layer; 10 for adding hot water outlet, and 11 is helical heating pipe, and 12 is the salinity tester; 13 are the heating water-in; 14 are the emptying bucket, and 15 is aeration tube, and 16 is blow-down pipe.
Embodiment
Below in conjunction with accompanying drawing embodiment of the present utility model is described.
The little algae that the utility model provides is cultivated the bioreactor structure as shown in Figure 1, comprises that mainly light source 1, light source disperse tube 2, biological respinse tube 4, emptying bucket 14, pillar 7 and support chassis 8.Light source 1 is installed in the top that awl tubular light source is dispersed tube 2, and light source is dispersed the upper end that tube 2 back-offs are installed in biological respinse tube 4 cylindraceous; Disperse the middle part of tube 2 at light source Bright Source Protection layer 9 is installed, disperse on tube 2 walls at the light source of Bright Source Protection layer 9 bottom a ventpipe 3 is installed; Spirrillum heating tube 11 is installed in the biological respinse tube 4, and the water outlet 10 of the water-in 13 of heating tube lower end and heating tube upper end all stretches out outside the wall of biological respinse tube 4; Temperature measuring device 5, basicity tester 6 and salinity tester 12 are installed in the biological respinse tube 4, and the control panel of above-mentioned three testers is installed in respectively on the outer wall of biological respinse tube 4; The lower end of biological respinse tube 4 is by the fixedly connected awl tubular emptying bucket 14 of flange, and point mouth place, emptying bucket 14 bottoms connects the blow-down pipe 16 that valve is housed, and emptying bucket 14 is installed two aeration tubes 15 that valve is housed near symmetry on the outer wall of sharp mouth; Three pillars 7 equidistantly are supported on biological respinse tube 4 and emptying bucket 14 joining places, and pillar 7 lower ends are fixed on the support chassis 8.
In the utility model, light source is dispersed tube 2 and is designed to bore tubular, and light source 1 generally can adopt the cold lamp of the metal halide that has the white fluorescent coating, is installed in the top that light source is dispersed tube 2, so that the light full illumination is in biological respinse tube 4.For preventing that the water vapour in the biological respinse tube 4 from causing damage to light source 1; light source disperse the tube 2 the tube in medium position; one planar transparent Bright Source Protection layer 9 is installed, also can on Bright Source Protection layer 9, be placed some shade materials, to regulate the intensity of illumination in the biological respinse tube 4.Disperse on tube 2 the barrel at the light source of Bright Source Protection layer below 9 ventpipe 3 is housed, play effect, also can be used as the dosing mouth of salinity and basicity in the conditioned reaction device simultaneously into the reactor ventilation.
Light source is dispersed tube 2, biological respinse tube 4 and emptying bucket 14 are transparent material (as organic glass) and make, inwall scribbles waterproof minute surface light reflecting material, perhaps outer wall covers the minute surface light reflecting material, guarantees to improve the utilising efficiency of light in the equal reflected back biological respinse of the light tube 4 that light source sends.
When helical heating pipe 11 is installed, the central lines of its helical centre and biological respinse tube 4.
Begin light source to be dispersed tube 2 and remove before little algae cultivation, in bio-reactor 4 and emptying bucket 14, inject nutrient solution, insert the algae kind, again light source is dispersed tube 2 and installed.Open light source 1 and salinity tester 12, basicity tester 6 and temperature measuring device 5, every day regularly carries out gaseous interchange by 15 pairs of reactors of aeration tube and nutrient solution mixes.When temperature is low, can utilizes hot water to pass through helical heating pipe 11 and be the reactor heating.Salinity and basicity are the important factors that influences micro algae growth, when these conditions are not suitable for micro algae growth, can pass through ventpipe 3 dosing in reaction solution, are fit to micro algae growth to adjust salinity and basicity.Should make reaction solution evenly mixed by aeration after the dosing.When micro algae growth arrives to a certain degree, just can entire reaction liquid be emitted to gather in the crops little algae by blow-down pipe 16.
Experiment showed, that the utility model can improve the optical efficiency of bio-reactor, gaseous interchange is convenient, mixes, and temperature of reaction is stable, can improve output and the quality of little algae.

Claims (4)

1, a kind of little algae is cultivated bioreactor, it is characterized in that comprising that light source (1), light source disperse tube (2), a biological respinse tube (4), emptying bucket (14), pillar (7) and support chassis (8), light source (1) is installed in the top that awl tubular light source is dispersed tube (2), and light source is dispersed the upper end that tube (2) back-off is installed in biological respinse tube cylindraceous (4); Disperse the middle part of tube (2) at light source Bright Source Protection layer (9) is installed, disperse on tube (2) wall at the light source of Bright Source Protection layer (9) bottom a ventpipe (3) is installed; Spirrillum heating tube (11) is installed in the biological respinse tube (4), and the water outlet (10) of water-in of heating tube lower end (13) and heating tube upper end all stretches out outside the wall of biological respinse tube (4); Temperature measuring device (5), basicity tester (6) and salinity tester (12) are installed in the biological respinse tube (4), and the control panel of above-mentioned three testers is installed in respectively on the outer wall of biological respinse tube (4); The lower end of biological respinse tube (4) is connected and installed awl tubular emptying bucket (14), and point mouth place, emptying bucket (14) bottom connects the blow-down pipe (16) that valve is housed, and emptying bucket (14) is installed two aeration tubes (15) that valve is housed near symmetry on the outer wall of sharp mouth; Three pillars (7) equidistantly are supported on biological respinse tube (4) and emptying bucket (14) joining place, and pillar (7) lower end is fixed on the support chassis (8).
2, cultivate bioreactor according to the little algae of claim 1, it is characterized in that described light source disperses tube (2), biological respinse tube (4) and emptying bucket (14) and be transparent material and make, inwall scribbles or outer wall covering minute surface light reflecting material.
3, cultivate bioreactor according to little algae of claim 1, it is characterized in that the height of described biological respinse tube (4) and the ratio of cylinder section diameter are 1:0.8~1.2.
4, cultivate bioreactor according to little algae of claim 1, it is characterized in that the helical centre and biological respinse tube (4) central lines of described helical heating pipe (11).
CNU2008200571065U 2008-04-10 2008-04-10 Photobioreactor for microalgae cultivation Expired - Fee Related CN201245657Y (en)

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Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101942383A (en) * 2010-08-05 2011-01-12 南京工业大学 Photobioreactor
CN102071134A (en) * 2010-12-11 2011-05-25 福州大学 Spirulina light emitting diode (LED) photobioreactor
CN102226146A (en) * 2011-05-20 2011-10-26 金华职业技术学院 Photobiological reaction equipment of microalgae
CN101629141B (en) * 2009-08-21 2012-07-04 新奥科技发展有限公司 System and method for keeping temperature of microalgae suspension and photosynthesis reactor
CN102575211A (en) * 2009-10-20 2012-07-11 瓦克化学股份公司 Tubular photobioreactor
CN104164359A (en) * 2014-07-23 2014-11-26 刘跃魁 Microorganism fermentation device
CN104797700A (en) * 2012-08-14 2015-07-22 康姆顿合伙制公司 Photobioreactor for cultivating phototrophic organisms
CN105670925A (en) * 2016-03-11 2016-06-15 江苏明昌生物科技有限公司 Intelligent photosynthetic bacterium fermentation device for aquaculture
TWI615467B (en) * 2013-12-30 2018-02-21 國立交通大學 Microalgae harvest module and method of harvesting microalgae
CN108485983A (en) * 2018-06-04 2018-09-04 海南源泉生物科技有限公司 A kind of culture medium and its cultural method for cultivating green alga for scale high density
CN110358669A (en) * 2019-07-29 2019-10-22 湘南学院 A kind of algae peculiar smell acquisition device and peculiar smell acquisition method

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101629141B (en) * 2009-08-21 2012-07-04 新奥科技发展有限公司 System and method for keeping temperature of microalgae suspension and photosynthesis reactor
CN102575211A (en) * 2009-10-20 2012-07-11 瓦克化学股份公司 Tubular photobioreactor
CN101942383B (en) * 2010-08-05 2013-04-10 南京工业大学 Photobioreactor
CN101942383A (en) * 2010-08-05 2011-01-12 南京工业大学 Photobioreactor
CN102071134A (en) * 2010-12-11 2011-05-25 福州大学 Spirulina light emitting diode (LED) photobioreactor
CN102226146A (en) * 2011-05-20 2011-10-26 金华职业技术学院 Photobiological reaction equipment of microalgae
CN104797700A (en) * 2012-08-14 2015-07-22 康姆顿合伙制公司 Photobioreactor for cultivating phototrophic organisms
TWI615467B (en) * 2013-12-30 2018-02-21 國立交通大學 Microalgae harvest module and method of harvesting microalgae
CN104164359A (en) * 2014-07-23 2014-11-26 刘跃魁 Microorganism fermentation device
CN105670925A (en) * 2016-03-11 2016-06-15 江苏明昌生物科技有限公司 Intelligent photosynthetic bacterium fermentation device for aquaculture
CN108485983A (en) * 2018-06-04 2018-09-04 海南源泉生物科技有限公司 A kind of culture medium and its cultural method for cultivating green alga for scale high density
CN108485983B (en) * 2018-06-04 2021-07-30 海南源泉生物科技有限公司 Culture medium for large-scale high-density cultivation of green algae and culture method thereof
CN110358669A (en) * 2019-07-29 2019-10-22 湘南学院 A kind of algae peculiar smell acquisition device and peculiar smell acquisition method

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Granted publication date: 20090527

Termination date: 20100410