CN110358669A - A kind of algae peculiar smell acquisition device and peculiar smell acquisition method - Google Patents
A kind of algae peculiar smell acquisition device and peculiar smell acquisition method Download PDFInfo
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- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 claims description 3
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 9
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Abstract
本发明公开了一种藻类异味采集装置，包括柱式培养瓶、光源、曝气装置、风机、气体流量计、吸附瓶。柱式培养瓶通过通气管与曝气装置连接，在柱式培养瓶顶部安装有可控光源并通过光源控制器调节。柱式培养瓶与吸附瓶通过风机利用收集管连接，气体流量计可对风速及时间进行设定。收集管内可放置不同吸附特性的吸附材料。本发明可对不同藻类生长条件进行光照强度和曝气强度设置，同时利用曝气带动水中异味物质的释放。该方法最大程度减少异味物质挥发，不需提取藻液采用原位采集的方式更能准确的反应藻细胞在生长过程中异味物质释放情况，具有简单、真实、易操作等特点，满足实验室条件下最大程度获取藻源性异味物质的需求。
The invention discloses an algae odor collection device, which comprises a column culture bottle, a light source, an aeration device, a fan, a gas flow meter and an adsorption bottle. The column culture bottle is connected with the aeration device through the air pipe, and a controllable light source is installed on the top of the column culture bottle and adjusted by the light source controller. The column culture bottle and the adsorption bottle are connected through a fan and a collection tube, and the gas flow meter can set the wind speed and time. Adsorbent materials with different adsorption characteristics can be placed in the collecting tube. The invention can set the light intensity and aeration intensity for different algae growth conditions, and at the same time utilize the aeration to drive the release of odorous substances in water. This method minimizes the volatilization of odorous substances and does not need to extract algae liquid. The method of in-situ collection can more accurately reflect the release of odorous substances during the growth of algal cells. It is simple, real, and easy to operate, and meets laboratory conditions. The demand for maximizing the acquisition of algae-derived odor substances.
Description
技术领域technical field
本发明涉及藻类生物学研究领域，具体涉及一种藻类异味采集装置及采集方法。The invention relates to the field of algae biology research, in particular to an algae odor collection device and collection method.
背景技术Background technique
异味物质(Taste and Odor,T&O)是指能被人感觉器官(鼻、口和舌)感受到的令人讨厌的或异常的气味。引起水体异味的原因有工农业的废水污染以及水体的富营养化等。近年来研究表明藻类是导致水源中异味发生的主要原因，在富营养化水体中，由于营养物的过剩，使得一些藻、菌过量繁殖。大量的藻、菌繁殖不仅影响水体的正常使用，而且产生各种次级代谢产物，如藻毒素和异味物质。其中蓝藻、绿藻、硅藻等均能产生相应的异味。据有关报道，从目前分离确认的藻类来看，已有200多种藻源性异味物质，蓝藻尤其是丝状蓝藻所产生的异味物质占已知异味藻的25％以上，其中产土霉味异味的藻类分布最为广泛。因此，蓝藻被认为水体异味的主要生产者，消除因蓝藻繁殖而带来的藻源异味物作为常规的水处理方法。Taste and Odor (T&O) refers to an unpleasant or abnormal smell that can be felt by human sensory organs (nose, mouth and tongue). The causes of water body odor include industrial and agricultural wastewater pollution and eutrophication of water bodies. In recent years, studies have shown that algae are the main cause of odor in water sources. In eutrophic water bodies, due to excess nutrients, some algae and bacteria multiply excessively. The reproduction of a large number of algae and bacteria not only affects the normal use of water bodies, but also produces various secondary metabolites, such as algal toxins and odorous substances. Among them, cyanobacteria, green algae, and diatoms can all produce corresponding odors. According to related reports, judging from the currently isolated and confirmed algae, there are more than 200 algae-derived odorous substances, and the odorous substances produced by cyanobacteria, especially filamentous cyanobacteria, account for more than 25% of the known odorous algae. Odor-smelling algae are the most widely distributed. Therefore, cyanobacteria are considered to be the main producers of water body odors, and the elimination of algae-sourced odors caused by the reproduction of cyanobacteria is a conventional water treatment method.
目前，异味物质的检测是采用不同的前处理方法对异味物质进行富集，再结合气质联用仪(GC-MS)来定性分析，因此富集方法的灵敏度主要取决于样品前处理的富集效率。常用的富集方法有蒸汽蒸馏法、液-液萃取法、闭环捕集法、开环捕集法、固相萃取法等。然而异味物质作为挥发性有机物，在样品富集过程中为了最大程度体现样品真实值，富集时间相对会增加。然而随着富集时间的延长，富集效率也不断下降。不仅如此，在实验室条件对藻类异味物质监测过程中发现，大量异味物质被释放到空气中，实际检测结果与理论值存在很大差异。因此针对以上问题，建立一种可靠准确的藻类异味采集方法一直是异味物质的研究热点。本发明将藻类生长过程与吸附装置结合，无需将藻细胞脱离原生环境，利用吸附瓶实时对异味物质进行富集，摆脱了传统富集过程中时间周期长、结果不准确的缺点，更有利于研究藻类异味释放的过程。At present, the detection of odor substances is to use different pretreatment methods to enrich the odor substances, and then combine with gas chromatography-mass spectrometry (GC-MS) for qualitative analysis. Therefore, the sensitivity of the enrichment method mainly depends on the enrichment of the sample pretreatment. efficiency. Commonly used enrichment methods include steam distillation, liquid-liquid extraction, closed-loop trapping, open-loop trapping, and solid-phase extraction. However, odorous substances are volatile organic compounds. In order to reflect the true value of the sample to the greatest extent during the sample enrichment process, the enrichment time will be relatively increased. However, as the enrichment time prolongs, the enrichment efficiency also decreases continuously. Not only that, but in the process of monitoring algae odor substances under laboratory conditions, it was found that a large amount of odor substances were released into the air, and the actual test results were quite different from the theoretical values. Therefore, in view of the above problems, establishing a reliable and accurate algae odor collection method has always been a research hotspot of odor substances. The invention combines the algae growth process with the adsorption device, without the need to separate the algae cells from the original environment, and uses the adsorption bottle to enrich the odorous substances in real time, which gets rid of the shortcomings of long time period and inaccurate results in the traditional enrichment process, and is more beneficial Study the process of algal odor release.
目前尚未检索到藻类异味采集装置及方法的报道和使用。The report and use of the algae odor collection device and method have not been retrieved yet.
发明内容Contents of the invention
本发明的目的之一在于提供一种藻类异味采集装置，解决现有技术中存在的不能准确反应藻细胞在生长过程中异味物质释放的问题。One of the objectives of the present invention is to provide a device for collecting algae odor, which solves the problem in the prior art that it cannot accurately reflect the release of odorous substances during the growth of algae cells.
本发明提供的藻类异味采集装置，包括通过收集管连通的培养瓶、吸附瓶，培养瓶为柱式培养瓶，所述柱式培养瓶上设置可控光源以及曝气装置；所述收集管管道上安装有风机、气体流量计；柱式培养瓶通过通气管与曝气装置连接；通过柱式培养瓶对产异味藻类进行培养，利用光源和曝气装置为藻类光合作用提供光照和二氧化碳(CO2)。气体流量计可对风机的风速及时间进行设定。所述收集管管道一端伸入柱式培养瓶，另一端伸入至吸附瓶的底部，吸附瓶的底部设置不同吸附特性的吸附材料。本发明可对不同藻类生长条件进行光照强度和曝气强度设置，同时利用曝气带动水中异味物质的释放。The algae odor collection device provided by the present invention comprises a culture bottle and an adsorption bottle connected through a collection tube, the culture bottle is a column culture bottle, and a controllable light source and an aeration device are arranged on the column culture bottle; A fan and a gas flow meter are installed on the top; the column culture bottle is connected to the aeration device through the vent pipe; the odor-producing algae are cultivated through the column culture bottle, and the light source and the aeration device are used to provide light and carbon dioxide (CO2) for algae photosynthesis. 2 ). The gas flow meter can set the wind speed and time of the fan. One end of the collection pipe extends into the column culture bottle, and the other end extends into the bottom of the adsorption bottle, and the bottom of the adsorption bottle is provided with adsorption materials with different adsorption properties. The invention can set the light intensity and aeration intensity for different algae growth conditions, and at the same time utilize the aeration to drive the release of odorous substances in water.
所述柱式培养瓶整体为密封结构，由透明有机玻璃材料制成，整体呈圆柱形，直径200-300mm，高度400-600mm，柱式培养瓶顶端有机玻璃盖可通过螺纹与柱式培养瓶连接。The column culture bottle has a sealed structure as a whole and is made of transparent organic glass material. It is cylindrical as a whole, with a diameter of 200-300mm and a height of 400-600mm. connect.
所述光源安装在柱式培养瓶顶端，光源系统由20-80W全光谱波长400-840nm LED灯泡组成，LED灯泡以柱式培养瓶顶部圆心为中心呈圆形向四周分布，灯泡与安装在柱式培养瓶上的光源控制器电路连接，通过光源控制器对灯泡的光照强度和波长进行设定。The light source is installed on the top of the column culture bottle. The light source system is composed of 20-80W full-spectrum wavelength 400-840nm LED bulbs. The LED bulbs are distributed around the center of the column culture bottle top in a circle. The light source controller circuit on the type culture bottle is connected, and the light intensity and wavelength of the bulb are set through the light source controller.
所述吸附瓶为40-80ml螺纹口瓶，通过螺纹口盖与收集管连接。The adsorption bottle is a 40-80ml screw-top bottle, which is connected with a collection tube through a screw-top cap.
所述曝气装置功率为40-60w，排气量20-40L/min。The power of the aeration device is 40-60w, and the displacement is 20-40L/min.
所述通气管为L型玻璃导管，总长度500-800mm，直径4-6mm，一端与曝气装置连接，另一端与柱式培养瓶连接，通气管距离柱式培养瓶底端高度为10-30mm。The ventilation tube is an L-shaped glass conduit with a total length of 500-800mm and a diameter of 4-6mm. One end is connected to the aeration device, and the other end is connected to the column culture bottle. The height of the ventilation tube from the bottom of the column culture bottle is 10- 30mm.
所述收集管为U型玻璃导管，总长度300-500mm，直径4-6mm，一端与吸附瓶连接，另一端与柱式培养瓶连接，收集管距离柱式培养瓶顶端高度为5-10mm，距离吸附材料高度10-30mm。The collection tube is a U-shaped glass catheter with a total length of 300-500mm and a diameter of 4-6mm. One end is connected to the adsorption bottle, and the other end is connected to the column culture bottle. The height of the collection tube from the top of the column culture bottle is 5-10mm. 10-30mm from the height of the absorbent material.
所述吸附材料包括活性炭、沸石。The adsorption material includes activated carbon and zeolite.
本发明的另一个目的提供了一种利用上述藻类异味采集装置进行异味采集的方法，该方法包括以下步骤：Another object of the present invention provides a method for collecting peculiar smell by using the above-mentioned algae peculiar smell collecting device, the method comprising the following steps:
将分离的产异味藻类接种于柱式培养瓶，柱式培养瓶含BG11培养基，在20μmol/(m2.s)、(25±1)℃、光照周期12h:12h(L:D)的条件下，24h不间断曝气培养，通气流量设置为5L/min；取5g活性炭或沸石作为吸附剂，设置风机流量4L/min，吸附时间30min，每隔5d进行取样分析。Inoculate the isolated odor-producing algae into a column culture bottle containing BG11 medium, and inoculate them at 20 μmol/(m 2 .s), (25±1)°C, and light cycle 12h:12h (L:D) Under these conditions, 24 hours of uninterrupted aeration culture, the ventilation flow rate is set to 5L/min; 5g of activated carbon or zeolite is used as the adsorbent, the fan flow rate is set to 4L/min, the adsorption time is 30min, and samples are taken for analysis every 5 days.
有益效果：Beneficial effect:
1.本发明可应用于藻类培养过程中的异味采集，实时对异味物质释放情况进行监测。1. The present invention can be applied to the collection of peculiar smell in the process of algae cultivation, and monitor the release of peculiar smell substances in real time.
2.根据不同藻类的生长特性设定不同光照强度与波长，方便了解藻细胞异味随环境变化过程中异味释放情况。2. Set different light intensities and wavelengths according to the growth characteristics of different algae, which is convenient for understanding the odor release of algae cells during the process of changing the environment.
3.在异味物质检测过程中，最大程度减少异味物质的损耗，更能真实的反应真实值。3. During the detection of odorous substances, the loss of odorous substances is minimized, and the true value can be more truly reflected.
4.异味采集成本相比较现有技术成本大大降低，不会对环境造成危害，操作更加方便。4. Compared with the cost of the existing technology, the cost of odor collection is greatly reduced, it will not cause harm to the environment, and the operation is more convenient.
附图说明Description of drawings
通过阅读下文优选实施方式的详细描述，各种其他的优点和益处对于本领域普通技术人员将变得清楚明了。附图仅用于示出优选实施方式的目的，而并不认为是对本发明的限制。在附图中：Various other advantages and benefits will become apparent to those of ordinary skill in the art upon reading the following detailed description of the preferred embodiment. The drawings are only for the purpose of illustrating a preferred embodiment and are not to be considered as limiting the invention. In the attached picture:
图1为本发明所述一种藻类异味采集装置示意图；Fig. 1 is a schematic diagram of an algae odor collection device according to the present invention;
图2为实施例二测定藻细胞土臭素释放量图。Fig. 2 is the graph of measuring the release amount of geosmin in algae cells in Example 2.
其中1-柱式培养瓶，2-风机，3-吸附瓶，4-光源控制器，5-光源，6-曝气装置，7-通气管，8-气体流量计，9-收集管，10-藻液，11-吸附材料。Among them, 1-column culture bottle, 2-fan, 3-adsorption bottle, 4-light source controller, 5-light source, 6-aeration device, 7-air pipe, 8-gas flow meter, 9-collection tube, 10 - algae liquid, 11 - adsorption material.
具体实施方式Detailed ways
以下将参考附图并结合实施例来详细说明本发明。The present invention will be described in detail below with reference to the accompanying drawings and examples.
实施例1Example 1
参照图1，一种藻类异味采集装置示意图，其特征在于：包括柱式培养瓶(1)、风机(2)、吸附瓶(3)、光源控制器(4)、光源(5)、曝气装置(6)、通气管(7)、气体流量计(8)、收集管(9)、藻液(10)、吸附材料(11)。柱式培养瓶(1)整体为密封结构，由透明有机玻璃材料制成，整体呈圆柱形，直径200mm，高度400mm。柱式培养瓶(1)顶端有机玻璃盖可通过螺纹与柱式培养瓶(1)连接。方便添加藻液(10)。柱式培养瓶(1)顶端安装有35w全光谱波长400-840nm LED灯泡，并通过光源控制器(4)进行控制。通气管(7)为L型玻璃导管，总长度600mm，直径5mm，一端与曝气装置(6)连接，另一端与柱式培养瓶(1)连接，通气管(7)距离柱式培养瓶(1)底端高度为10mm。曝气装置(6)功率为40w，排气量20L/min。吸附瓶(3)为40mL螺纹口瓶，通过螺纹口盖与收集管(9)连接。收集管(9)为U型玻璃导管，总长度500mm，直径5mm，一端与吸附瓶(3)连接，另一端与柱式培养瓶(1)连接，在通气管中部装有风机(2)与气体流量计(8)，风机(2)功率为20w，排气量10L/min。收集管(9)距离柱式培养瓶(1)顶端高度为5mm，距离吸附材料(11)高度10mm。在异味采集之前向吸附瓶(3)中加5g活性炭作为吸附剂。With reference to Fig. 1, a kind of algae peculiar smell collecting device schematic diagram is characterized in that: comprise column culture bottle (1), blower fan (2), adsorption bottle (3), light source controller (4), light source (5), aeration A device (6), a ventilation pipe (7), a gas flow meter (8), a collecting pipe (9), an algal solution (10), and an adsorption material (11). The column culture bottle (1) has a sealed structure as a whole, is made of transparent plexiglass material, and is cylindrical as a whole, with a diameter of 200 mm and a height of 400 mm. The plexiglass cover at the top of the column culture bottle (1) can be connected with the column culture bottle (1) through threads. It is convenient to add algae solution (10). A 35w full-spectrum wavelength 400-840nm LED light bulb is installed on the top of the column culture bottle (1), and is controlled by a light source controller (4). The vent pipe (7) is an L-shaped glass conduit with a total length of 600 mm and a diameter of 5 mm. One end is connected to the aeration device (6), and the other end is connected to the column culture bottle (1). The distance between the vent pipe (7) and the column culture bottle (1) The height of the bottom end is 10mm. Aeration device (6) power is 40w, displacement 20L/min. The adsorption bottle (3) is a 40mL screw-top bottle, which is connected to the collection tube (9) through a screw-top cap. The collection pipe (9) is a U-shaped glass conduit with a total length of 500mm and a diameter of 5mm. One end is connected to the adsorption bottle (3), and the other end is connected to the column culture bottle (1). Gas flow meter (8), blower fan (2) power is 20w, displacement 10L/min. The height of the collection tube (9) from the top of the column culture bottle (1) is 5 mm, and the height from the adsorption material (11) is 10 mm. Add 5g of activated carbon as an adsorbent in the adsorption bottle (3) before the peculiar smell is collected.
在实际操作过程中要根据培养藻类的种类合理选择LED光源光照强度和波长并根据异味物质特征选择吸附材料。In the actual operation process, the light intensity and wavelength of the LED light source should be reasonably selected according to the type of algae to be cultivated, and the adsorption material should be selected according to the characteristics of the odor substance.
实施例2Example 2
对分离的一株产土臭素(Geosmin)蓝藻进行产异味特征分析。将该株蓝藻接种于含BG11培养基的柱式培养瓶，在20μmol/(m2.s)、(25±1)℃、光照周期12h:12h(L:D)的条件下，24h不间断曝气培养，通气流量设置为5L/min。每隔5d进行取样分析，取5g活性炭作为吸附剂，设置风机流量4L/min，吸附时间30min。The odor-producing characteristics of an isolated Geosmin-producing cyanobacteria were analyzed. Inoculate the strain of cyanobacteria into a column culture flask containing BG11 medium, under the conditions of 20 μmol/(m 2 .s), (25±1)°C, and a photoperiod of 12h:12h (L:D), 24h uninterrupted For aeration culture, the aeration flow rate was set at 5 L/min. Sampling and analysis were carried out every 5 days, 5g of activated carbon was taken as the adsorbent, the flow rate of the fan was set to 4L/min, and the adsorption time was 30min.
将实施例2中吸附完成后的活性炭装入40mL螺纹口瓶中，加入25ml超纯水、8g氯化钠利用顶空固相萃取法(HS-SPME)进行萃取。在萃取完成后，拔出萃取针，插入气相进样室解吸5min。测定藻细胞生长过程异味物质释放量。结果见图2，随着藻细胞的生长，土臭素释放量不断增加，20天时达到最大值，随后不断下降。表明该方法能正确反应藻细胞生长过程异味物质释放情况。Put the activated carbon after adsorption in Example 2 into a 40mL screw-top bottle, add 25ml of ultrapure water, and 8g of sodium chloride for extraction by headspace solid-phase extraction (HS-SPME). After the extraction is completed, pull out the extraction needle and insert it into the gas phase sampling chamber for desorption for 5 minutes. Determination of the release of odorous substances during the growth of algae cells. The results are shown in Figure 2. With the growth of algal cells, the release of geosmin increased continuously, reached the maximum at 20 days, and then decreased continuously. It shows that this method can correctly reflect the release of odorous substances during the growth of algae cells.
上述内容仅为本发明典型实施方式，并且易于作出其他多种变化而不超出本发明权利要求中描述的准确范围。The above are only typical implementations of the present invention, and other various changes can be easily made without departing from the precise scope described in the claims of the present invention.
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