CN104073435A - Movable all-weather microalgae light complement bioreaction culture system and culture method - Google Patents
Movable all-weather microalgae light complement bioreaction culture system and culture method Download PDFInfo
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- CN104073435A CN104073435A CN201410310484.XA CN201410310484A CN104073435A CN 104073435 A CN104073435 A CN 104073435A CN 201410310484 A CN201410310484 A CN 201410310484A CN 104073435 A CN104073435 A CN 104073435A
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- 230000000295 complement Effects 0.000 title abstract 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 45
- 241000195493 Cryptophyta Species 0.000 claims abstract description 42
- 235000011089 carbon dioxide Nutrition 0.000 claims abstract description 42
- 239000007788 liquid Substances 0.000 claims abstract description 31
- 238000003756 stirring Methods 0.000 claims abstract description 18
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 10
- 239000001301 oxygen Substances 0.000 claims abstract description 10
- MYMOFIZGZYHOMD-UHFFFAOYSA-N oxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 7
- 235000015097 nutrients Nutrition 0.000 claims abstract description 7
- 239000012530 fluid Substances 0.000 claims description 17
- 238000005286 illumination Methods 0.000 claims description 17
- 238000001802 infusion Methods 0.000 claims description 17
- 238000010438 heat treatment Methods 0.000 claims description 11
- 230000000050 nutritive Effects 0.000 claims description 7
- 230000001954 sterilising Effects 0.000 claims description 7
- 238000000034 method Methods 0.000 claims description 6
- 239000011229 interlayer Substances 0.000 claims description 5
- 230000003287 optical Effects 0.000 claims description 5
- 239000001569 carbon dioxide Substances 0.000 abstract description 3
- 150000003839 salts Chemical class 0.000 abstract description 3
- 239000011780 sodium chloride Substances 0.000 abstract description 3
- 238000004140 cleaning Methods 0.000 abstract description 2
- 238000009423 ventilation Methods 0.000 abstract 2
- 230000004154 complement system Effects 0.000 abstract 1
- 101700039143 LAMP2 Proteins 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000003670 easy-to-clean Effects 0.000 description 2
- 239000011521 glass Substances 0.000 description 2
- 230000000258 photobiological Effects 0.000 description 2
- 230000001502 supplementation Effects 0.000 description 2
- 230000001488 breeding Effects 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 150000004676 glycans Polymers 0.000 description 1
- 239000003317 industrial substance Substances 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000029553 photosynthesis Effects 0.000 description 1
- 238000010672 photosynthesis Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 150000004804 polysaccharides Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000001105 regulatory Effects 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/52—Mobile; Means for transporting the apparatus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/26—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pH
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
- C12M41/34—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas
Abstract
The invention discloses a movable all-weather microalgae light complement bioreaction culture system and a culture method. The culture system mainly comprises a light complement bioreaction tank, wherein a tank body is configured with a ventilation system consisting of a carbon dioxide ventilation pipe in the tank body and an oxygen outlet in the upper part of the tank body; a light complement system consisting of a plurality of uniformly distributed long-wave and short-wave LED lamps and a light intensity control switch is arranged in the tank body; a heater, a temperature meter and a stirring system consisting of a stirring paddle and a motor connected with the stirring paddle are also mounted in the tank body; a liquid complement hole capable of feeding dry ice is formed in the upper part of the tank body, and an algae liquid collection connector is arranged at the lower part of the tank body. According to the culture system, excellent conditions are provided for growth and propagation of microalgae by complementing light to adjust the light intensity and the wavelength, controlling the temperature, introducing carbon dioxide, adding an optimized nutrient salt formula, stirring algae liquid and adjusting and monitoring the pH value of culture liquid, so that quick culture of the microalgae is realized; the culture system has the characteristics of being simple in structure, convenient to operate, high in culture efficiency, low in cost, convenient to clean, capable of increasing the microalgae yield, and the like.
Description
The round-the-clock micro-algae light filling biological respinse culture systems of a kind of packaged type and cultural method.
Technical field
The present invention relates to the round-the-clock micro-algae light filling biological respinse culture systems of a kind of packaged type and cultural method, belong to micro-algae and cultivate optical-biological reaction technical field.
Technical background
Micro-algae is the ancient lower plant of a class, is distributed widely in the waters such as ocean, freshwater lake.At present, the known micro-algae kind in the whole world reaches kind more than 20,000.The nutritive ingredient and the industrial chemicals that in microalgae cell, contain multiple high value, cellular metabolism can produce polysaccharide, protein, pigment etc., at aspects such as medicine industry, health care of food product industry, animal-feed, cosmetic industries, will have extensive exploitation and application prospect.
Bioreactor is the core of micro-algae industrialization, and it is the particularly guarantee of the micro-algae high yield of high added value, stable yields of micro-algae.At present, microalgae photobiological reactor mainly contains tubular type, flat, raceway pond, the cleaning difficulty that the complex structure that they have, the complex operation having, the cost having be expensive, have, easily breaking down of having, especially existing microalgae photobiological reactor all can not effectively be worked, or effectively work at night in cloudy, rainy day.
Summary of the invention
The object of the invention is to overcome the deficiency that prior art exists, and provide a kind of simple in structure, easy to operate, culture efficiency is high, with low cost, easy to clean, the while can be improved again round-the-clock micro-algae light filling biological respinse culture systems and the cultural method of micro-algae output.
The object of the invention is to complete by following technical solution, described round-the-clock micro-algae light filling biological respinse culture systems, it mainly comprises a light filling biological reactor, tank body disposes the blow and vent system consisting of the oxygen outlet of offering on the carbonic acid gas ventpipe in tank body and tank body, is provided with the illumination replenishment system consisting of a plurality of uniform long waves and shortwave LED lamp and intensity of illumination trip switch in tank body; The stirring system that well heater and thermometer is also installed in tank body and is formed with the motor that is connected by stirring rake; Described tank body top is provided with the fluid infusion mouth that can access dry ice, and the bottom of tank body is provided with algae liquid and collects interface.
Top in described tank body is provided with the ultraviolet lamp for sterilizing, and bottom is provided with Ph meter, and forms a Ph regulation system with CO2 flow controller, and movable base and the universal wheel moving for tank body is separately installed in the bottom of tank body; Described tank body is comprised of cylindrical tank and truncated cone-shaped upper cover, and the internal diameter of described tank body is 0.3-1m, is highly 0.8-1.5m, and upper cover lower diameter is identical with retort main body, is highly 0.1-0.3m.
The bottom of described tank body, sidewall and upper cover adopt respectively sandwich structure, and described long wave and shortwave LED lamp are installed in interlayer, or long wave and the shortwave LED lamp of waterproof are directly installed in described tank body; It is 600-700nm light that the LED lamp of described long wave can send wavelength, and the intensity of illumination of long wave light source is at 1000-10000Lx, and the LED lamp of described shortwave can send the light that wavelength is 400-500nm, and the intensity of illumination of shortwave light source is at 1000-20000Lx.
Micro-algae light filling biological respinse cultural method is carried out in a kind of utilization as mentioned above round-the-clock micro-algae light filling biological respinse culture systems of packaged type, it is, by movable base, light filling bio-reactor is moved to specified location, close algae liquid and collect mouth, open oxygen outlet, open ultraviolet lamp sterilizing 20min; Close ultraviolet lamp, the nutritive medium preparing is imported to light filling biological reactor by fluid infusion mouth; Algae kind is accessed to light filling biological reactor by fluid infusion mouth; Turn on longwave optical (640nm left and right) LED lamp, according to the required light intensity of different algae kinds, turn on the LED lamp of different quantities; Connect carbonic acid gas ventpipe, pass into carbonic acid gas; Open motor and stir algae liquid, start to cultivate; In culturing process, can be according to practical situation by fluid infusion mouth Additional nutrient solution, observe the temperature of thermometer demonstration and the pH value on PH meter, heating rod energising heating when temperature is lower, when temperature is higher, can add appropriate dry ice to control algae liquid temp by dry ice access port, when PH is higher, strengthen carbonic acid gas intake, when PH is lower, reduce carbonic acid gas intake; After cultivation completes, open algae liquid and collect interface, collect algae liquid.
The round-the-clock microalgae culture system of packaged type of the present invention, it have simple in structure, easy to operate, culture efficiency is high, with low cost, easy to clean, simultaneously can improve the features such as micro-algae output again.It by supplementing light source capable of regulating illumination intensity and wavelength, control temperature, pass into carbonic acid gas, add the nutritive salt formula optimized, stir algae liquid and regulate monitoring nutrient solution pH value to provide good condition for micro algae growth breeding, realize micro-algae fast culture.Utilizing native system can realize micro-algae cultivates throughout the year, can avoid Yin Gaowen under natural condition, low temperature, the overcast and rainy disadvantageous effect to micro algae growth, also can under field conditions (factors), utilize native system to extend micro-algae incubation time, shorten micro-algae culture cycle, improve micro-algae output.
Accompanying drawing explanation
Fig. 1 is the structural representation of round-the-clock micro-algae light filling biological respinse culture systems of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention will be described in detail: shown in Fig. 1, round-the-clock micro-algae light filling biological respinse culture systems of the present invention, it mainly comprises a light filling biological reactor 7, tank body disposes the blow and vent system consisting of the oxygen outlet 4 of offering on the carbonic acid gas ventpipe 1 in tank body and tank body, is provided with the illumination replenishment system consisting of a plurality of uniform long waves and shortwave LED lamp 6 and intensity of illumination trip switch in tank body; The stirring system that well heater 8 and thermometer 11 is also installed in tank body and is formed with the motor that is connected by stirring rake 3; Described tank body top is provided with the fluid infusion mouth 5 that can add dry ice, access algae kind, and the bottom of tank body is provided with algae liquid and collects interface 10.
Top in described tank body is provided with the ultraviolet lamp 2 for sterilizing, and bottom is provided with Ph meter 9, and forms a Ph regulation system with CO2 flow controller, and movable base 13 and the universal wheel 12 moving for tank body is separately installed in the bottom of tank body; Described tank body is comprised of cylindrical tank and truncated cone-shaped upper cover, and the internal diameter of described tank body is 0.3-1m, is highly 0.8-1.5m, and upper cover lower diameter is identical with retort main body, is highly 0.1-0.3m.
The bottom of described tank body, sidewall and upper cover adopt respectively sandwich structure, and described long wave and shortwave LED lamp are installed in interlayer, or long wave and the shortwave LED lamp of waterproof are directly installed in described tank body; It is 600-700nm light that the LED lamp of described long wave can send wavelength, and the intensity of illumination of long wave light source is at 1000-10000Lx, and the LED lamp of described shortwave can send the light that wavelength is 400-500nm, and the intensity of illumination of shortwave light source is at 1000-20000Lx.
Micro-algae light filling biological respinse cultural method is carried out in a kind of utilization as mentioned above round-the-clock micro-algae light filling biological respinse culture systems, it is, by movable base 13, light filling bio-reactor is moved to specified location, close algae liquid and collect mouth 10, open oxygen outlet 4, open ultraviolet lamp 2 sterilizing 20min; Close ultraviolet lamp, the nutritive medium preparing is imported to light filling biological reactor 7 by fluid infusion mouth 5; Algae kind is accessed to light filling biological reactors by fluid infusion mouth 5; Turn on longwave optical (640nm left and right) LED lamp 6, according to the required light intensity of different algae kinds, turn on the LED lamp 6 of different quantities; Connect carbonic acid gas ventpipe 1, pass into carbonic acid gas; Open motor and stir algae liquid, start to cultivate; In culturing process, can be according to practical situation by fluid infusion mouth 5 Additional nutrient solutions, observe the temperature of thermometer 11 demonstrations and the pH value on PH meter 9, heating rod 8 energising heating when temperature is lower, when temperature is higher, can add appropriate dry ice to control algae liquid temp by dry ice access port 5, when PH is higher, strengthen carbonic acid gas intake, when PH is lower, reduce carbonic acid gas intake; After cultivation completes, open algae liquid and collect interface 10, collect algae liquid.
The present invention can solve the particularly problem of the micro-algae cultivation of high added value aspect of micro-algae, also can solve in scientific research, and the on-the-spot problem of cultivating of collection in worksite algae kind, portability is used to scientific research place.
Embodiment: the round-the-clock micro-algae light filling bioreactor culture system of a kind of packaged type of the present invention, it mainly comprises:
One light filling biological reactor, retort inner major diameter is 0.3-1m, is highly 0.8-1.5m, upper cover lower diameter is identical with retort main body, is highly 0.1-0.3m;
One blow and vent system, is comprised of carbon dioxide pipeline and oxygen outlet, for providing micro algae growth to breed required carbon and getting rid of the oxygen that micro-algae photosynthesis produces;
One illumination replenishment system, the bottom of optical-biological reaction tank, sidewall and upper cover are with interlayer, be used for installing light source, also can remove interlayer water-proof light source is directly installed, light source divides two kinds, and one is 640nm left and right light for sending wavelength, one for sending the light that wavelength is about 450nm, the intensity of illumination of long wave light source is at 1000-10000Lx, and the intensity of illumination of shortwave light source is at 1000-20000Lx, and the light source that adopts which kind of wavelength is determined by the cultivation stage of micro-algae;
One temperature control system, is added mouthful and thermometer forms by heating rod, dry ice, and heating rod heats use during for low temperature, can design temperature, and dry ice is lowered the temperature during for summer high temperature;
One fluid infusion system, is comprised of liquid supplementation channel and fluid infusion interface, for accessing algae kind and Additional nutrient solution.
One stirring system, is comprised of motor and stirring rake, can set rotating speed, for stirring algae liquid, mixes carbonic acid gas, nutritive salt and makes micro-algae have equal opportunity to touch LED light;
One PH regulation system, is comprised of PH meter and CO2 flow controller, by adjusting, adds the amount of carbonic acid gas to control the pH value of micro algae culturing liquid;
One movable base, is comprised of dull and stereotyped and universal wheel mounted thereto, for mobile light filling bio-reactor.
The main body of described light filling biological reactor is cylinder, and upper cover is round platform; Described light filling biological reactor is made by transparent materials such as PC, PVC, PE, synthetic glass, glass; The light source of described illumination replenishment system is LED plant growth lamp, and temperature control system heats up and controlled by heating rod, cooling dry ice.
In above structure, the large I of light filling biological reactor customizes according to actual needs; Carbonic acid gas air flow is cultivated required adjusting size according to micro-algae; LED lamp is installed according to the required photochromic and light intensity of micro-algae culturing process, basic index longwave optical in vegetative period, exponential growth later stage short wavelength light.
The specific works process of described light filling bio-reactor is: through movable base 13, light filling bio-reactor is moved to specified location, close algae liquid and collect mouth 10, open oxygen outlet 4, open ultraviolet lamp 2 sterilizing 20min; Close ultraviolet lamp, the nutritive medium preparing is imported to light filling biological reactor 7 by fluid infusion mouth 5; Algae kind is accessed to light filling biological reactors by fluid infusion mouth 5; Turn on longwave optical (640nm left and right) LED lamp 6, according to the required light intensity of different algae kinds, turn on the LED lamp 6 of different quantities; Connect carbonic acid gas ventpipe 1, pass into carbonic acid gas; Open motor and stir algae liquid, start to cultivate; In culturing process, can be according to practical situation by fluid infusion mouth 5 Additional nutrient solutions, observe the temperature of thermometer 11 demonstrations and the pH value on PH meter 9, heating rod 8 energising heating when temperature is lower, when temperature is higher, can add appropriate dry ice to control algae liquid temp by dry ice access port 5, when PH is higher, strengthen carbonic acid gas intake, when PH is lower, reduce carbonic acid gas intake; After cultivation completes, open algae liquid and collect interface 10, collect algae liquid.
Claims (4)
1. the round-the-clock micro-algae light filling biological respinse culture systems of packaged type, it is characterized in that: it mainly comprises a light filling biological reactor, tank body disposes the blow and vent system consisting of the oxygen outlet of offering on the carbonic acid gas ventpipe in tank body and tank body, is provided with the illumination replenishment system consisting of a plurality of uniform long waves and shortwave LED lamp and intensity of illumination trip switch in tank body; The stirring system that well heater and thermometer is also installed in tank body and is formed with the motor that is connected by stirring rake; Described tank body top is provided with the fluid infusion mouth that can access dry ice, and the bottom of tank body is provided with algae liquid and collects interface.
2. the round-the-clock micro-algae light filling biological respinse culture systems of packaged type according to claim 1, it is characterized in that: the top in described tank body is provided with the ultraviolet lamp for sterilizing, bottom is provided with Ph meter, and form a Ph regulation system with CO2 flow controller, movable base and the universal wheel moving for tank body is separately installed in the bottom of tank body; Described tank body is comprised of cylindrical tank and truncated cone-shaped upper cover, and the internal diameter of described tank body is 0.3-1m, is highly 0.8-1.5m, and upper cover lower diameter is identical with retort main body, is highly 0.1-0.3m.
3. the round-the-clock micro-algae light filling biological respinse culture systems of packaged type according to claim 1 and 2, it is characterized in that: the bottom of described tank body, sidewall and upper cover adopt respectively sandwich structure, and described long wave and shortwave LED lamp are installed in interlayer, or long wave and the shortwave LED lamp of waterproof are directly installed in described tank body; It is 600-700nm light that the LED lamp of described long wave can send wavelength, and the intensity of illumination of long wave light source is at 1000-10000Lx, and the LED lamp of described shortwave can send the light that wavelength is 400-500nm, and the intensity of illumination of shortwave light source is at 1000-20000Lx.
4. a utilization micro-algae light filling biological respinse culture systems as round-the-clock in packaged type as described in claim 1 or 2 or 3 is carried out micro-algae light filling biological respinse cultural method, it is characterized in that: it is, by movable base, light filling bio-reactor is moved to specified location, close algae liquid and collect mouth, open oxygen outlet, open ultraviolet lamp sterilizing 20min; Close ultraviolet lamp, the nutritive medium preparing is imported to light filling biological reactor by fluid infusion mouth; Algae kind is accessed to light filling biological reactor by fluid infusion mouth; Turn on longwave optical LED lamp, according to the required light intensity of different algae kinds, turn on the LED lamp of different quantities; Connect carbonic acid gas ventpipe, pass into carbonic acid gas; Open motor and stir algae liquid, start to cultivate; In culturing process, can be according to practical situation by fluid infusion mouth Additional nutrient solution, observe the temperature of thermometer demonstration and the pH value on PH meter, heating rod energising heating when temperature is lower, when temperature is higher, can add appropriate dry ice to control algae liquid temp by dry ice access port, when PH is higher, strengthen carbonic acid gas intake, when PH is lower, reduce carbonic acid gas intake; After cultivation completes, open algae liquid and collect interface, collect algae liquid.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104312901A (en) * | 2014-10-20 | 2015-01-28 | 杭州鑫伟低碳技术研发有限公司 | Microalgae cultivation module and large-scale microalgae factory cultivation system |
| CN105779268A (en) * | 2014-12-23 | 2016-07-20 | 中国石油化工股份有限公司 | Apparatus and method for culturing photosynthetic microorganisms |
| CN106281984A (en) * | 2016-08-14 | 2017-01-04 | 江苏翔康海洋生物科技有限公司 | New and effective domestic micro algae culturing device |
| CN107227252A (en) * | 2017-07-03 | 2017-10-03 | 深圳市华明佰利科技有限公司 | A kind of vertical LED luminous energy biological respinse kettle of totally-enclosed |
| CN109652299A (en) * | 2018-11-15 | 2019-04-19 | 上海量能生物科技有限公司 | Bioreactor with resistance to movement function |
| CN113150976A (en) * | 2021-05-13 | 2021-07-23 | 云南爱尔发生物技术股份有限公司 | Intensive cultivation system for microalgae |
| CN113150975A (en) * | 2021-03-26 | 2021-07-23 | 中国科学院半导体研究所 | Photobioreactor and method for microalgae growth |
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| CN106281984A (en) * | 2016-08-14 | 2017-01-04 | 江苏翔康海洋生物科技有限公司 | New and effective domestic micro algae culturing device |
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| CN109652299A (en) * | 2018-11-15 | 2019-04-19 | 上海量能生物科技有限公司 | Bioreactor with resistance to movement function |
| CN113150975A (en) * | 2021-03-26 | 2021-07-23 | 中国科学院半导体研究所 | Photobioreactor and method for microalgae growth |
| CN113150976A (en) * | 2021-05-13 | 2021-07-23 | 云南爱尔发生物技术股份有限公司 | Intensive cultivation system for microalgae |
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