CN107460115A - It is sustained feed supplement bioreactor and microalgae culture method - Google Patents
It is sustained feed supplement bioreactor and microalgae culture method Download PDFInfo
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- CN107460115A CN107460115A CN201710606893.8A CN201710606893A CN107460115A CN 107460115 A CN107460115 A CN 107460115A CN 201710606893 A CN201710606893 A CN 201710606893A CN 107460115 A CN107460115 A CN 107460115A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
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Abstract
The invention discloses one kind sustained release feed supplement bioreactor and microalgae culture method, the specific bioreactor is the floatation type bioreactor with sustained release feed supplementing device and semipermeable membrane device, and can provide sufficient nutrients by plus slow release feed supplementing device supports microalgae efficient growth.For the high salinity brought when avoiding and supplementing bicarbonate and high pH problems, the bioreactor, which immerses water body part, has semipermeable membrane device, the salt ion and hydroxide ion of high concentration is accumulate to for discharging, to stablize salinity and pH in reactor.The present invention solves the problems, such as that nutriment is supplied during microdisk electrode Cost Problems and pH are difficult to stablize, and has good application prospect in terms of microalgae large-scale culture cost reduction.
Description
Technical field
The present invention relates to technical field of microalga biology, and in particular to bioreactor, passes through pellicle or capillary reality
Existing sustained release feed supplement bioreactor and microalgae culture method.
Background technology
Microalgae cell is rich in protein, polysaccharide, lipid, pigment and a variety of inorganic elements (such as Cu, Fe, Se, Mn, Zn)
Etc. the nutritional ingredient of high value, can be used for producing food, health products, feed, biochemicals and bio-fuel-oil.Meanwhile
It can be used for handling waste water and fixed carbon dioxide.
Bioreactor can be divided into open cell system and Closed photobioreactor at present, compared with open pond,
Closed photobioreactor has the advantages that contamination resistance is strong, moisture evaporation is few, condition of culture is relatively controllable, is trained in microalgae
It is huge to support aspect application potential.Micro algae growth requires supplementation with carbon dioxide, and nutrient solution is due to direct with air in open cell system
Contact, can not obtain carbon dioxide, but closed reactor system must be by way of ventilation into local from air
This process is completed, and this energy consumption is very high, causes cost increase.This problem can be by adding the side of high concentration bicarbonate
Formula solves, but general microalgae is difficult to bear salinity rise that the addition of high concentration bicarbonate brings and pH raises problem.With
Bicarbonate is similar, and the addition of other nutritional ingredients also brings along Similar Problems.
The content of the invention
The problems such as being raised for the salinity rise brought when solving addition high concentration bicarbonate and other nutrients and pH, this
A kind of floatation type bioreactor with sustained release feed supplementing device of disclosure of the invention, in such a system, by being sustained feed supplement knot
Structure unit can be provided sufficient bicarbonate and other nutriments for microalgae in bioreactor.Meanwhile to avoid mending
The high salinity brought during bicarbonate and high pH problems are filled, the bioreactor, which immerses water body part, has semipermeable membrane device,
The salt ion and hydroxide ion of high concentration are accumulate to for discharging, to stablize salinity and pH in reactor.
Floatation type bioreactor of the present invention, by reactor cavity, it is arranged at the half of reactor cavity bottom
Permeable membrane and sustained release feed supplement construction unit composition, wherein：
1) the sustained release feed supplement construction unit, for supplementing the nutriment quickly consumed during microdisk electrode；Specifically
Supplement bicarbonate and other nutrients are sustained for micro algae growth；Such as：Nitrogen source, phosphorus source, and it is other needed for micro algae growth
Trace element ion, such as：Bicarbonate, phosphate, nitrate, iron, calcium, magnesium, potassium, manganese, zinc, silicon, boron, cobalt, molybdenum etc..
2) bioreactor, which immerses water body part, has pellicle construction unit；For in releasing reactor cavity
Because sustained release feed supplement build up to high concentration salt ion and hydroxide ion into extraneous water body, to stablize inside bioreactor
The pH of water body.
For floatation type bioreactor described above, in the case of preferable, the sustained release feed supplement construction unit is
Closed container, the valve that the closed container passes through pellicle, capillary or adjustable flow velocity with the bioreactor cavity
Through connection.
For floatation type bioreactor described above, in the case of preferable, the semipermeable membrane material is selected from CA (two
Cellulose acetate)；CTA (Triafol T)；CN (nitrocellulose)；EC (ethyl cellulose)；CN-CA (composite fibre
Element), the hydrophilic nanoporous material such as PA-6 (nylon 6) or PA-66 (nylon) microporous barrier.
For floatation type bioreactor described above, in the case of preferable, the sustained release feed supplement construction unit
Pellicle, it is the pellicle that optimal area is calculated through formula (I), to realize the thing in reactor between nutrient solution and water body
Mass transter, to ensure micro algae growth.In addition, capillary or adjustable flow velocity described in the alternative that provides of the present invention
Valve, the slow release speed of the nutriment inside sustained release feed supplement construction unit can also be controlled, so as to ensure micro algae growth.
For floatation type bioreactor described above, in the case of preferable, the sustained release feed supplement construction unit
The area of pellicle can be estimated by below equation (likewise, how to replace with the valve of capillary or adjustable flow velocity,
Following algorithms can also be used for reference to be estimated so that it is determined that correct flow control), certain nutriment growth rate and film surface
Relational expression between product is represented by：
For certain nutriment by pellicle, the speed into reactor is represented by：
V is liquid volume in reactor (L) in formula, and K is the film infiltration coefficient (m to certain material2min-1), A is semi-transparent
Membrane area (cm2), D is film thickness (m), CoutIt is certain material concentration (mol/L), C in external environmentinIt is nutriment anti-
Answer concentration in device (mol/L).
For nutriment, in reactor base consumption speed rs can using approximate representation as：
Wherein rs can be expressed from the next：
Wherein rx is cell growth rate, and Yx/s is cellulation and consumes the ratio of substrate, for inside reactor
The change procedure of nutriment, when material is equal to the wear rate of inside reactor substrate by the speed of pellicle, this
Process can be represented by the following formula：
Therefore, the relational expression between growth rate and required membrane area can be obtained by formula (3) and (4)：
It is described to be sustained in feed supplement construction unit in the case of preferable for floatation type bioreactor described above
To calculate the nutriment for microdisk electrode of gained concentration through formula (I).Nutrients in the sustained release feed supplement construction unit
Matter concentration is higher than the concentration of the corresponding nutriment in reactor cavity body, and the nutriment species includes：Nitrogen source, phosphorus source, with
And other trace element ions needed for micro algae growth, such as：Bicarbonate, phosphate, nitrate, iron, calcium, magnesium, potassium, manganese,
Zinc, silicon, boron, cobalt, molybdenum etc..
For floatation type bioreactor described above, in the case of preferable, the pellicle construction unit, including
With the pellicle of cavity wall detachable connection under bioreactor and the mechanism for fixing semi-transparent film edge.
For floatation type bioreactor described above, in the case of preferable, the pellicle construction unit is to lead to
Threaded connection, plug connection, the form that snaps connection or be fitting to connection are crossed by the frame or pellet type knot of the semi-transparent film edge of fixation
Structure unit and cavity wall detachable connection under bioreactor.Specifically, the edge of the frame or tray-like structure unit
For real frame, inside is net formula, fence type or porous structure, and the real frame is advantageous to fix pellicle, the inside be net formula,
On the one hand auxiliary holds pellicle for fence type or porous structure, and on the other hand, the setting of its hole or grid is without prejudice to semi-transparent
Mass exchange of the film to nutriment and water body.
In the case of preferred, the pellicle building unit structures include the screw rod being connected with lower cavity wall, and can lead to
Cross with nut pallet apparatus pellicle being pressed on screw rod.
For floatation type bioreactor described above, in the case of preferable, the shape of the bioreactor
For horizontal plate formula, reactor cavity has the space for housing microdisk electrode, and can float on water surface.
For floatation type bioreactor described above, in the case of preferable, the reactor cavity can be by energy
The rigid material of own form is maintained to be made, can also be by the flexible material of its shape can not be maintained to be made；It is described
Rigid material includes glass, rigid plastics etc., and the flexible material is including plastic sheeting etc..
Described reactor cavity is floated in the water surface by additional flotation gear.
Another aspect of the present invention is, discloses a kind of microalgae culture method, and this method uses either one described above
The bioreactor culture microalgae of case.
Beneficial effects of the present invention：
When using high-concentration nutrient feed supplement, the salinity in reactor can be caused to rise problem.Such as utilize carbonic acid
Hydrogen sodium carry out feed supplement when, Na ion concentration can due to feed supplement and concentration raise, and bicarbonate radical can be converted into carbon dioxide and
Hydroxyl, the former transforms into biomass by photosynthesis, and remaining hydroxide ion can cause pH to raise.Have in the present invention
The semipermeable membrane device of mass exchange is carried out with extraneous water body, too high Na ion concentration can be discharged, maintenance reaction device inner salt is dense
Degree balance；It is also possible to discharge hydroxide ion, pH is stable in maintenance reaction device.Semipermeable membrane device can also discharge other
Material prevents its accumulation in the reactor into water body.For example, suppression own growth may be produced during micro algae growth
Material, can also be by semipermeable membrane into water body.Certainly, the nutriment in extraneous water body can also penetrate into reaction
In device, for micro algae growth.
Brief description of the drawings
Fig. 1, rigid floatation type pellicle bioreactor sustained release feed supplement construction unit are shown using semipermeable membrane device structure
It is intended to；Wherein, 1. cavity, 2. pellicles, 3. sustained release feed supplement construction unit bottles, 4. pellicle pallets, 5. spiral fixing devices.
Fig. 2, marine chlorella cultivate schematic diagram in four kinds of different reactors；Ease up including with semipermeable membrane device
Feed supplement construction unit reactor is released, the reactor only with semipermeable membrane device, only there is the reactor of sustained release feed supplement construction unit,
Common floatation type reactor, under same condition of culture, cultivate the chlorella dry weights (1) of 10 days gained, pH (2), in reactor
Portion's TIC concentration (3), total nitrogen concentration (4), total phosphorus concentration (5) change, all data are to be obtained under two parallel laboratory tests
Mean+/-standard error.
Embodiment
Following non-limiting examples can make one of ordinary skill in the art be more fully understood the present invention, but not with
Any mode limits the present invention.The description below be only to this application claims scope exemplary illustration, this area skill
Art personnel can make a variety of changes and modification according to disclosure of that to present invention, and it should also be as belonging to this Shen
Among the scope that please be claimed.
Embodiment 1.
Floatation type pellicle bioreactor sustained release feed supplement construction unit uses semipermeable membrane device
As shown in figure 1, the reactor includes the cavity 1 of culture marine chlorella, cavity 1 fills high concentration nutritional with inside
Between the sustained release feed supplement construction unit bottle 3 of material, it is fixed by spiral fixing device 5 and cavity 1, is sustained feed supplement structure list
First bottle 3 is insertion with cavity 1, and the two joint compresses pellicle 2, and nutriment is by being sustained feed supplement in sustained release feed supplementing device
Construction unit bottle 3 enters cavity 1, in addition, the position that pellicle pallet apparatus 4 is corresponded on the outer wall of cavity 1 is open, pellicle
Pellicle 2 is pressed on cavity 1 by pallet apparatus 4, cavity 1, by the pellicle 2 on pellicle pallet apparatus 4, by cavity 1
The salt ion and hydroxide ion of the interior paramount concentration of accumulation are discharged into extraneous water body, to stablize water body inside bioreactor
PH.
Embodiment 2
Floatation type bioreactor sustained release feed supplement construction unit uses semipermeable membrane device culture microalgae
Using the bioreactor 1 described in embodiment 1, three groups of controls are set respectively, only there is semipermeable membrane device
Floatation type bioreactor, the floatation type bioreactor only with sustained release feed supplement construction unit and common floatation type
Bioreactor is in outside scenery marine chlorella, wherein the concentration of phosphorus is 35mmol/L, nitrogen in sustained release feed supplement construction unit
Concentration is 60mmol/L, concentration of carbon 0.12mol/L, and other culture medium prescriptions are identical with seawater component.Temperature and illumination are not done
Any artificial treatment, is completely dependent on natural conditions.
As shown in Figure 2, the reactor shown in the embodiment of the present invention 1 can successfully be cultivated micro- cultivation results in outdoor conditions
Algae, it can be seen from Fig. 2 (1) in four kinds of reactors, while there is the reaction of sustained release feed supplement construction unit and semipermeable membrane device
Device dry weight will be far above other reactors, up to 0.82g/L, be with semipermeable membrane device reactor maximum dry weight only
0.61g/L, the reactor maximum dry weight for being only sustained feed supplement construction unit is 0.60g/L, and blank control group highest only has
For 0.31g/L, dry weight increase is fairly obvious.Fig. 2 (2) then represents the change of medium pH inside different reactor, can by figure
Slower to find out while have the reactor pH of sustained release feed supplement construction unit and semipermeable membrane device to rise, final pH is empty 9.58
PH has reached 10.01 in white control group.What Fig. 2 (3) was represented is the content of TIC in reactor, while has sustained release feed supplement
The reactor TIC of construction unit and semipermeable membrane device maintains 3mmol/L, only has the total nothing of semipermeable membrane device reactor
Machine concentration of carbon maintains 1.51mmol/L, and the reactor TIC concentration for being only sustained feed supplement construction unit maintains
1.31mmol/L, blank control group is almost depleted, illustrates while has sustained release feed supplement construction unit and semipermeable membrane device
Reactor, which has, maintains the effect of TIC concentration.What same Fig. 2 (4) was represented is the content of total nitrogen in reactor, Fig. 2 (5) table
Show the content of total phosphorus in reactor.The reactor total nitrogen with sustained release feed supplement construction unit and semipermeable membrane device maintains
0.2mmol/L, total phosphorus maintain 0.05mmol/L, only depleted with semipermeable membrane device reactor total nitrogen and total phosphorus, only
The reactor total nitrogen concentration for having sustained release feed supplement construction unit is 0.20mmol/L, and total phosphorus also has 0.03mmol/L, blank control group
Almost depleted, these data explanation, the bioreactor with sustained release feed supplement construction unit and semipermeable membrane device can be with
Maintain stable pH stable, and maintain better nutritivity thing concentration, and therefore obtain higher microalgae biomass concentration, realize compared with
The culture of optimization.
Thus, floatation type bioreactor of the present invention, its structure are adapted to the microdisk electrode of scale, well solution
Determined traditional microdisk electrode the problem of, reduce the cost that nutriment in microdisk electrode is supplied, effective and stable controls
The pH of reactor, there is good application prospect in terms of microalgae large-scale culture cost reduction.
Claims (11)
- A kind of 1. floatation type bioreactor, it is characterised in that：By reactor cavity, it is arranged at the half of reactor cavity bottom Permeable membrane and sustained release feed supplement construction unit composition, wherein：1) the sustained release feed supplement construction unit, too fast nutriment is consumed during microdisk electrode for supplementing；2) bioreactor, which immerses water body part, has pellicle construction unit；For highly concentrated in releasing reactor cavity The salt ion and hydroxide ion of degree.
- 2. floatation type bioreactor according to claim 1, it is characterised in that：It is described sustained release feed supplement construction unit be Closed container, the valve that the closed container passes through pellicle, capillary or adjustable flow velocity with the bioreactor cavity Through connection.
- 3. floatation type bioreactor according to claim 1 or 2, it is characterised in that：The semipermeable membrane material is selected from Cellulose diacetate, Triafol T, nitrocellulose, ethyl cellulose, cellulose mixture or nylon.
- 4. floatation type bioreactor according to claim 1, it is characterised in that：In the sustained release feed supplement construction unit To calculate the nutriment of gained concentration through formula (I), the nutrient concentrations being sustained in feed supplement construction unit are higher than reaction The concentration of corresponding nutriment in device cavity,<mrow> <mi>A</mi> <mo>=</mo> <mfrac> <mrow> <msub> <mi>r</mi> <mi>x</mi> </msub> <mo>.</mo> <mi>D</mi> <mo>&CenterDot;</mo> <mi>V</mi> </mrow> <mrow> <mi>K</mi> <mo>&CenterDot;</mo> <mrow> <mo>(</mo> <msub> <mi>C</mi> <mrow> <mi>o</mi> <mi>u</mi> <mi>t</mi> </mrow> </msub> <mo>-</mo> <msub> <mi>C</mi> <mrow> <mi>i</mi> <mi>n</mi> </mrow> </msub> <mo>)</mo> </mrow> <mo>&CenterDot;</mo> <msub> <mi>Y</mi> <mrow> <mi>x</mi> <mo>/</mo> <mi>s</mi> </mrow> </msub> </mrow> </mfrac> <mo>-</mo> <mo>-</mo> <mo>-</mo> <mrow> <mo>(</mo> <mi>I</mi> <mo>)</mo> </mrow> <mo>.</mo> </mrow>
- 5. floatation type bioreactor according to claim 1, it is characterised in that：In the sustained release feed supplement construction unit Membrane area needed for pellicle calculates through formula (I).
- 6. floatation type bioreactor according to claim 1, it is characterised in that：The pellicle construction unit, bag Include and the pellicle of cavity wall detachable connection under bioreactor and the mechanism for fixing semi-transparent film edge.
- 7. floatation type bioreactor according to claim 6, it is characterised in that：The pellicle construction unit is, It is connected through a screw thread, plugs connection, the form for snapping connection or being fitting to connection by the frame or pellet type of the semi-transparent film edge of fixation Construction unit and cavity wall detachable connection under bioreactor.
- 8. floatation type bioreactor according to claim 1, it is characterised in that：The shape of the bioreactor For horizontal plate formula, reactor cavity has the space for housing microdisk electrode, and can float on water surface.
- 9. floatation type bioreactor according to claim 1, it is characterised in that：The bioreactor is by that can tie up The rigid material for holding own form or the flexible material that can not maintain own form make.
- 10. floatation type bioreactor according to claim 1, it is characterised in that：The flexible material is that plastics are thin Film, the rigid material are selected from glass, rigid plastics.
- 11. a kind of microalgae culture method, it is characterised in that this method is using described in claim 1-10 any one claim Bioreactor culture microalgae.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710606893.8A CN107460115B (en) | 2017-07-24 | 2017-07-24 | Slow-release material-supplementing photobioreactor and microalgae culture method |
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| CN201710606893.8A CN107460115B (en) | 2017-07-24 | 2017-07-24 | Slow-release material-supplementing photobioreactor and microalgae culture method |
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| CN107460115A true CN107460115A (en) | 2017-12-12 |
| CN107460115B CN107460115B (en) | 2021-08-31 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109912136A (en) * | 2019-05-05 | 2019-06-21 | 南昌大学 | A method of utilizing adsorption treatment ammonia nitrogen waste water culture microalgae |
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| CN1814745A (en) * | 2005-12-15 | 2006-08-09 | 中国人民解放军军事医学科学院野战输血研究所 | Cell culturation apparatus |
| KR20120021567A (en) * | 2010-08-09 | 2012-03-09 | 인하대학교 산학협력단 | Photobioreactor equipped with photocatalysts and method for cultivating photosynthetic microalgae using the same |
| US20130052719A1 (en) * | 2010-02-22 | 2013-02-28 | Inha-Industry Partnership Institute | Photobioreactor for mass culture of microalgae, and method for culturing microalgae by using same |
| CN103205361A (en) * | 2013-04-22 | 2013-07-17 | 青岛中仁药业有限公司 | Aerosol microalgae illuminating reaction device |
| CN105209593A (en) * | 2013-04-05 | 2015-12-30 | 仁荷大学校产学协力团 | Photobioreactor for mass culturing of photosynthetic microorganism |
| WO2017051334A1 (en) * | 2015-09-22 | 2017-03-30 | Aljadix Ag | Apparatus and process for sea surface microalgae cultivation |
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| CN1814745A (en) * | 2005-12-15 | 2006-08-09 | 中国人民解放军军事医学科学院野战输血研究所 | Cell culturation apparatus |
| US20130052719A1 (en) * | 2010-02-22 | 2013-02-28 | Inha-Industry Partnership Institute | Photobioreactor for mass culture of microalgae, and method for culturing microalgae by using same |
| KR20120021567A (en) * | 2010-08-09 | 2012-03-09 | 인하대학교 산학협력단 | Photobioreactor equipped with photocatalysts and method for cultivating photosynthetic microalgae using the same |
| CN105209593A (en) * | 2013-04-05 | 2015-12-30 | 仁荷大学校产学协力团 | Photobioreactor for mass culturing of photosynthetic microorganism |
| CN103205361A (en) * | 2013-04-22 | 2013-07-17 | 青岛中仁药业有限公司 | Aerosol microalgae illuminating reaction device |
| WO2017051334A1 (en) * | 2015-09-22 | 2017-03-30 | Aljadix Ag | Apparatus and process for sea surface microalgae cultivation |
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| CN107460115B (en) | 2021-08-31 |
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