CN202595126U - Primary culture device of microalgae - Google Patents

Primary culture device of microalgae Download PDF

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Publication number
CN202595126U
CN202595126U CN 201220235963 CN201220235963U CN202595126U CN 202595126 U CN202595126 U CN 202595126U CN 201220235963 CN201220235963 CN 201220235963 CN 201220235963 U CN201220235963 U CN 201220235963U CN 202595126 U CN202595126 U CN 202595126U
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CN
China
Prior art keywords
culture
little algae
level
lamp
plate
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Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 201220235963
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Chinese (zh)
Inventor
陆建学
高露姣
林听听
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East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
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East China Sea Fishery Research Institute Chinese Academy of Fishery Sciences
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Priority to CN 201220235963 priority Critical patent/CN202595126U/en
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/08Flask, bottle or test tube
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M27/00Means for mixing, agitating or circulating fluids in the vessel
    • C12M27/16Vibrating; Shaking; Tilting

Abstract

The utility model relates to a primary culture device of microalgae. The primary culture device comprises a culture box, an ultraviolet lamp, a full-spectrum plant tissue culture lamp, a shaking bed and microalgae primary culture bottles. The culture box is a transparent hollow cubic container provided with a framework, and a lateral plate on one side of the culture box is of a hinge door structure. The culture box is arranged on the upper surface of an operation table, and the ultraviolet lamp and the full-spectrum plant tissue culture lamp are arranged on the upper portion of a back lining plate in the culture box. The shaking bed is arranged on the upper surface of a base plate in the culture box, a main body of the shaking bed comprises a shaking bed plate and is assembled with an oscillating controller, a timer and a fixing clamp, a plurality of microalgae primary culture bottles are arranged on the upper surface of the shaking bed plate and fixed through the fixing clamp, and each microalgae primary culture bottle is matched with a plug. The primary culture device of microalgae is reasonable in design, compact in structure and convenient to operate and saves labor and energy, and the cultured microalgae are high in purity and cell density.

Description

The one-level culture apparatus of a kind of little algae
Technical field
The utility model belongs to biological feed and cultivates the field that learns a skill, and particularly relates to the one-level culture apparatus of a kind of little algae.
Background technology
Little algae is meant those small algae unicellular or that be made up of several cells.They are absorption of nutrients matter from seawater, utilizes luminous energy to carry out photosynthesis, makes the organism of forming cell, is the basic producer who constitutes marine food chain.Little algae commonly used in culture fishery mainly comprises unicellular algaes such as Chlorophyta, Bacillariophyta, Chrysophyta; This slightly algae have nutritious, size is agreeable to the taste, be difficult for advantage such as polluted water, is the important biomolecule bait of aquatic animals such as fish, shrimp, crab, shellfish.Little algae quantity has seldom satisfied not the needs that aquatic animal the best of high-density culture is bred and grown in natural sea-water, therefore needs artificial culture through preferred, as to have high nutritive value little algae.
The training method of little algae comprises that mainly purebred cultivation, one-level are cultivated, secondary is cultivated and three grades of cultivations (productivity cultivation).Purebred cultured microalgae generally is kept in the breadboard illumination box, and institute's cultured microalgae is for through separating the algae of the single variety select, and the few but based on very high purity of quantity does not have other assorted algae and exists, and mainly plays a part to purify and protect and plant; The one-level cultivation is to utilize the algae kind of purebred cultivation to carry out the enlarged culturing of the order of magnitude; Institute's cultured microalgae quantity increase and purity are higher; Play the effect that the algae kind is provided for secondary, three grades of cultivations, in little algae culturing process, have the advantages that to form a connecting link, the success or not meeting of its cultivation directly influences the success or failure that latter stage algae productivity is cultivated; Until having influence on breeding and growing of aquatic animal, therefore in whole algae culture program, has important effect.It generally is in the container of 250~5000mL, to carry out that one-level is cultivated; Under suitable illumination and temperature condition,, just can reach than high-cell density through 3~7 through replenishing the air and the nutritive salt of carbonic acid gas; When the one-level cultured microalgae reaches available concentration; Make succeeding transfer culture earlier, inoculate a small amount of algae kind (inoculum size how much depend on kind and cell density) in fresh nutrient solution, to continue keeping one-level to cultivate; Remaining most of one-level is cultivated algae liquid be inoculated in the large container of 20000~50000mL, the secondary that begins little algae is cultivated.
At present in the one-level culturing process of little algae, mainly be take every day regularly artificial hand shake that bottle stirs the mode of algae liquid or with common inflation mode culturing microalgae.Can guarantee that little algae has higher purity although shake the mode of the little algae of bottle stirring; But increased labour and energy widely, and algae liquid can not fully contact illumination, can not fully replenish carbonic acid gas and carry out photosynthesis; Thereby the algal grown cycle is longer, and algae cell density is not high yet; Common inflation can improve the culture density of algae; But tend to harmful organisms such as dust, bacterium, protozoon are brought in little algae through gas-filled valve; Culture environment is polluted, thereby made one-level cultivation and the follow-up cultivation of little algae have unstable and risk.Therefore, need provide a kind of equipment simple, easy to operate, can guarantee that little algae has higher purity and cell density, and can keep the one-level culture apparatus of the quick growth vigor of little algae.
Summary of the invention
The utility model technical problem to be solved provides the one-level culture apparatus of a kind of little algae, and the one-level that is used for little algae is cultivated, and can guarantee that little algae has higher purity and cell density, and can keep the quick growth vigor of little algae.
The utility model solves the technical scheme that its technical problem adopted: the one-level culture apparatus that a kind of little algae is provided; Comprise incubator, UV-lamp, full spectrum plant tissue culture lamp, shaking table, little algae one-level culturing bottle; Described incubator is the clear hollow cuboid container of tape frame, and one of which side side plate adopts the loose-leaf door; Described incubator places the operator's console upper surface, and rear lining plate top is provided with UV-lamp, full spectrum plant tissue culture lamp in the described incubator; Described incubator tank-top plate upper surface is placed shaking table; Described shaking table main part is the shaking table plate; And be furnished with oscillating controller, timer, retaining clip; Described shaking table plate upper surface is placed several little algae one-level culturing bottles, and fixes through retaining clip, and described little algae one-level culturing bottle is furnished with stopper.
Its framework of described incubator adopts stainless steel, is equipped with toughened glass side plate, toughened glass cover plate, stainless steel rear lining plate.
Described incubator length is 1300mm, and width is 800mm, highly is 750mm.
Described UV-lamp power is 15W.
Described full spectrum plant tissue culture lamp is the full spectrum plant tissue culture of a T5 type 10W lamp.
Described little algae one-level culturing bottle quantity is 2.
It is the Erlenmeyer flask of 5000mL that described little algae one-level culturing bottle adopts volume.
Described stopper is the cotton plug after sterilization.
Beneficial effect
The utility model is compared prior art and is had following beneficial effect: the one, and reasonable in design, simple for structure, easy to operate, utilize this device that shaking table and full spectrum lamp are stopped in setting-up time automatically or restart or operation continuously, save labour and energy; The 2nd, utilize this device can make microalgae cell in rocking, constantly contact the illumination and the carbon dioxide in air of full spectrum plant tissue culture lamp; Can utilize luminous energy and carbonic acid gas to promote photosynthesis fully; Accelerate micro algae growth speed; Cultivating under the factor condition identical with incubation time, institute's cultured microalgae cell density is to take 2.6~3.4 times of other training method gained concentration; The 3rd, can guarantee little algae purity, little algae purity is stabilized in more than 98%, far above 51~84% the purity of taking other training method gained.
Description of drawings
Fig. 1 is the utility model structural representation;
1. operator's console 2. incubators 3. UV-lamps 4. full spectrum plant tissue culture lamp 5. retaining clips 6. Erlenmeyer flasks 7. loose-leaf doors 8. shaking tables 9. shaking table plates 10. oscillating controllers 11. timers
Embodiment
Below in conjunction with specific embodiment, further set forth the utility model.Should be understood that these embodiment only to be used to the utility model is described and be not used in the restriction the utility model scope.Should be understood that in addition those skilled in the art can do various changes or modification to the utility model after the content of having read the utility model instruction, these equivalent form of values fall within the application's appended claims institute restricted portion equally.
As shown in Figure 1, the utility model comprises incubator 2, UV-lamp 3, full spectrum plant tissue culture lamp 4, shaking table 8, little algae one-level culturing bottle, and described incubator 2 is the clear hollow cuboid container of tape frame, and one of which side side plate adopts the loose-leaf door; Described incubator 2 places operator's console 1 upper surface, and rear lining plate top is provided with UV-lamp 3, full spectrum plant tissue culture lamp 4 in the described incubator 2; Described incubator 2 tank-top plate upper surfaces are placed shaking table 8; Described shaking table 8 main parts are shaking table plate 9; And be furnished with oscillating controller 10, timer 11, retaining clip 5; Described shaking table plate 9 upper surfaces are placed several little algae one-level culturing bottles, and fixing through retaining clip 5, and described little algae one-level culturing bottle is furnished with stopper.
Described incubator 2 its frameworks adopt stainless steel, are equipped with toughened glass side plate, toughened glass cover plate, stainless steel rear lining plate.
Described incubator 2 length are 1300mm, and width is 800mm, highly are 750mm.
Described UV-lamp 3 power are 15W.
Described full spectrum plant tissue culture lamp 4 is the full spectrum plant tissue culture of a T5 type 10W lamp.
Described little algae one-level culturing bottle quantity is 2.
It is the Erlenmeyer flask 6 of 5000mL that described little algae one-level culturing bottle adopts volume.
Described stopper is the cotton plug after sterilization.
When the utility model uses; The nutrient solution that at first splendid attire 3000mL crosses through autoclave sterilization in 5000mL Erlenmeyer flask 6; Get 500mL algae kind then and inoculate in the nutrient solution,, again Erlenmeyer flask 6 is positioned over shaking table plate 9 upper surfaces at the bottle neck of Erlenmeyer flask 6 disinfectant cotton plug beyond the Great Wall; After utilizing retaining clip 5 that Erlenmeyer flask 6 is fixed, the loose-leaf door 7 of the incubator 2 that closes.Turn on full spectrum plant tissue culture lamp 4; Start oscillating controller 10; According to the cultivation requirement of little algae, through regulating oscillating controller 10, to regulate the oscillation amplitude and the speed of shaking table 8; Algae liquid in the Erlenmeyer flask 6 is rocked with shaking table 8 vibrations; Thereby fully contact illumination and carbon dioxide in air to carry out photosynthesis, start timer 11 and can control shaking table 8 and full spectrum plant tissue culture lamp 4 at the same time or separately, setting-up time stopped automatically in the scope at 0~24 hour or restarts or operation continuously according to actual needs.Between during cultivation, start ultraviolet lamp disinfection every day one time, each disinfecting time is 30 minutes.After microalgae cell reaches higher density in the Erlenmeyer flask 6; The little algae of the 3000mL that gathers inoculates in other large container; The secondary that begins little algae is cultivated, and other gets 3000mL and in the nutrient solution that autoclave sterilization is crossed is poured Erlenmeyer flask 6 into after the little algae of residue mixes, begins succeeding transfer culture, or as required cultured little algae is all gathered; Other gets 500mL algae kind and inoculates in the 3000mL nutrient solution, and little algae one-level of a beginning new round is cultivated.

Claims (8)

1. the one-level culture apparatus of a little algae; Comprise incubator (2), UV-lamp (3), full spectrum plant tissue culture lamp (4), shaking table (8), little algae one-level culturing bottle; It is characterized in that: described incubator (2) is the clear hollow cuboid container of tape frame, and one of which side side plate adopts the loose-leaf door; Described incubator (2) places operator's console (1) upper surface, and the interior rear lining plate of described incubator (2) top is provided with UV-lamp (3), full spectrum plant tissue culture lamp (4); Described incubator (2) tank-top plate upper surface is placed shaking table (8); Described shaking table (8) main part is shaking table plate (9); And be furnished with oscillating controller (10), timer (11), retaining clip (5); Described shaking table plate (9) upper surface is placed several little algae one-level culturing bottles, and fixing through retaining clip (5), and described little algae one-level culturing bottle is furnished with stopper.
2. the one-level culture apparatus of a kind of little algae as claimed in claim 1 is characterized in that: its framework of described incubator (2) adopts stainless steel, is equipped with toughened glass side plate, toughened glass cover plate, stainless steel rear lining plate.
3. the one-level culture apparatus of a kind of little algae as claimed in claim 1, it is characterized in that: described incubator (2) length is 1300mm, and width is 800mm, highly is 750mm.
4. the one-level culture apparatus of a kind of little algae as claimed in claim 1, it is characterized in that: described UV-lamp (3) power is 15W.
5. the one-level culture apparatus of a kind of little algae as claimed in claim 1, it is characterized in that: described full spectrum plant tissue culture lamp (4) is the full spectrum plant tissue culture of a T5 type 10W lamp.
6. the one-level culture apparatus of a kind of little algae as claimed in claim 1, it is characterized in that: described little algae one-level culturing bottle quantity is 2.
7. the one-level culture apparatus of a kind of little algae as claimed in claim 1 is characterized in that: it is the Erlenmeyer flask (6) of 5000mL that described little algae one-level culturing bottle adopts volume.
8. the one-level culture apparatus of a kind of little algae as claimed in claim 1 is characterized in that: described stopper is the cotton plug after sterilizing.
CN 201220235963 2012-05-22 2012-05-22 Primary culture device of microalgae Expired - Fee Related CN202595126U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201220235963 CN202595126U (en) 2012-05-22 2012-05-22 Primary culture device of microalgae

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Application Number Priority Date Filing Date Title
CN 201220235963 CN202595126U (en) 2012-05-22 2012-05-22 Primary culture device of microalgae

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Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103849550A (en) * 2014-03-20 2014-06-11 环境保护部南京环境科学研究所 Secondary culture apparatus and culture method for alga
CN104109631A (en) * 2014-07-16 2014-10-22 中国科学院青岛生物能源与过程研究所 Microalgae culture reactor
CN104353390A (en) * 2014-11-07 2015-02-18 成都欧林生物科技股份有限公司 Device for conveniently mixing mixed liquid in serum bottle evenly
CN105381779A (en) * 2015-09-01 2016-03-09 河北爱能生物科技股份有限公司 Animal-derived membrane material processing apparatus
CN105400694A (en) * 2015-12-21 2016-03-16 天津亿海生物科技有限公司 Cell culture system
CN105706918A (en) * 2014-12-03 2016-06-29 中国水产科学研究院黄海水产研究所 Seaweed ultraviolet mutation breeding method and seaweed ultraviolet mutation breeding apparatus
CN106479892A (en) * 2016-10-21 2017-03-08 福建农林大学 A kind of high-light-energy utilization rate shaking table culture case for suspension culture
CN113621492A (en) * 2021-10-11 2021-11-09 天津长芦汉沽盐场有限责任公司 Spherical bottle shaking frame for algae cultivation

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103849550A (en) * 2014-03-20 2014-06-11 环境保护部南京环境科学研究所 Secondary culture apparatus and culture method for alga
CN103849550B (en) * 2014-03-20 2015-09-16 环境保护部南京环境科学研究所 A kind of algae secondary culture apparatus and cultural method thereof
CN104109631A (en) * 2014-07-16 2014-10-22 中国科学院青岛生物能源与过程研究所 Microalgae culture reactor
CN104353390A (en) * 2014-11-07 2015-02-18 成都欧林生物科技股份有限公司 Device for conveniently mixing mixed liquid in serum bottle evenly
CN105706918A (en) * 2014-12-03 2016-06-29 中国水产科学研究院黄海水产研究所 Seaweed ultraviolet mutation breeding method and seaweed ultraviolet mutation breeding apparatus
CN105381779A (en) * 2015-09-01 2016-03-09 河北爱能生物科技股份有限公司 Animal-derived membrane material processing apparatus
CN105381779B (en) * 2015-09-01 2017-06-13 河北爱能生物科技股份有限公司 A kind of animal derived film material processing unit
CN105400694A (en) * 2015-12-21 2016-03-16 天津亿海生物科技有限公司 Cell culture system
CN106479892A (en) * 2016-10-21 2017-03-08 福建农林大学 A kind of high-light-energy utilization rate shaking table culture case for suspension culture
CN106479892B (en) * 2016-10-21 2019-04-05 福建农林大学 A kind of high-light-energy utilization rate shaking table culture case for the culture that suspends
CN113621492A (en) * 2021-10-11 2021-11-09 天津长芦汉沽盐场有限责任公司 Spherical bottle shaking frame for algae cultivation

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CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20121212

Termination date: 20130522

C17 Cessation of patent right