A kind of high-light-energy utilization rate shaking table culture case for the culture that suspends
Technical field
The present invention relates to be related to optics culture device, especially a kind of high-light-energy utilization rate shaking table training for the culture that suspends
Support case.
Background technique
Suspend culture refer to it is a kind of in the fluid nutrient medium for constantly being stirred or being shaken, cultivate it is unicellular and it is small carefully
The tissue culture system of born of the same parents group, is a kind of training method of non-anchorage-dependent cell.Culture suspend in secondary metabolite
It is widely used in production, and there is unrivaled superiority.Under normal conditions, the standard taper of the laboratory culture used that suspends
The capacity of bottle is 150ml, 200ml, 250ml, and body outer diameter is respectively 69mm, 75mm, 85mm.Nowadays constant-temperature table is suspending
The utilization of culture is more and more extensive, but when studying influence of the light to the culture that suspends, many constant-temperature tables reach on the market
Less than requirement of experiment.The quality and secondary metabolites content of the culture that suspends, but shaking table on the market, lamp source can be improved in light
The problems such as unreasonable and conical flask the sealing polybag installed and designed shuts out the light, seriously affects the uniformity of illumination,
Experimental result is set to there is very big error.Therefore it is just very necessary to research and develop a high-light-energy utilization rate shaking table culture frame.
Lamp source design is the core of high-light-energy utilization rate shaking table.Particularity is cultivated due to suspending: the modeling of taper bottle closure
Material bag and conical flask are constantly being shifted one's position, and the uniformity of illumination can be all influenced.Constant-temperature table on the market at present, lamp source
Mounting design it is multifarious, be not able to satisfy illumination cultivation.Lamp bar is installed on side by some shaking tables, and some shaking tables are by lamp plate
It is installed on top, but is unable to solve the problems, such as optical uniformity;How there is the lamp of more excellent light environment for suspension Training Design
Source is a research direction.
LED light source is a kind of cold light source risen in recent years, has the advantages that space of economizing on electricity and save, partial monopoly is LED
Light source is used for incubator, and the light-emitting surface of LED light source is directional and light quantity is limited, need to there is the common work of a certain number of LED chips
Work be just easy luminous environment needed for reaching incubator, though however LED light source is that cold light source has certain operating temperature, such as cultivating
Largely LED light source is used in case there is a possibility that the temperature environment of incubator is not easy to control, how to solve these problems and one
Research direction.
Summary of the invention
The present invention proposes a kind of high-light-energy utilization rate shaking table culture case for the culture that suspends, and can be provided with LED light source can
The light environment of customization, and be not easy to influence box temperature because of heat of light source.
The present invention uses following technical scheme.
A kind of high-light-energy utilization rate shaking table culture case for the culture that suspends, the incubator includes cabinet, culturing rack group
Part, sterilization unit, temperature-controlling system unit, luminous environment control unit and control module, the control module and sterilization unit, temperature control
System unit is connected with luminous environment control unit, and cabinet is equipped with glass window;The culturing rack component includes rack, culture bottle
Fixed plate, lamp plate, lamp plate lifting module and shake module, the culture bottle fixed plate are horizontally placed in rack, and culture bottle is solid
Fixed board, which is equipped with, sets a bottle slot for fix taper culture bottle;The lamp plate level is set at the rack above culture bottle fixed plate
And be connected with lamp plate lifting module, lamp plate is equipped with multiple bottle holes probeed into for taper culture bottle top, the inner wall in each bottle of hole
Place is uniformly provided with multiple LED light sources;The luminous environment control unit includes light quality control unit, photoperiod control unit, light intensity
Control unit, light excluding shutter and antiglare module, the light quality control unit, intensity control unit are connected with the LED light source on lamp plate,
The light excluding shutter is set at glass window and is connected with antiglare module;The temperature-controlling system unit includes warming module, radiation air
Fan, exhaust fan and heating element, the air-out direction of radiator fan are directed toward culturing rack component, and the air-out direction of exhaust fan is directed toward cabinet
Outside, when the temperature inside the box that warming module measures exceeds threshold value, temperature-controlling system module controls the temperature inside the box;Work as culturing rack
It when component carries out culture operation, shakes module drive rack and shakes, lamp plate lifting module drive lamp plate is mobile to cover lamp plate bottle hole
At taper culture bottle, the LED light source at lamp plate bottle hole provides illumination to culture bottle, between lamp plate bottle hole and taper culture bottle outer wall
There are the gaps for forming heat dissipation wind path.
The illuminator of multiple LED light sources at the inner wall of the bottle hole is LED chip, and the LED chip includes red-light LED core
Piece, green LED chip and blue-light LED chip, multiple LED light sources are arranged in bottle hole inner wall punishment group by photochromic difference, are formed red
Light LED light strip, green light LED light belt and blue-ray LED light belt.
The light quality control unit, intensity control unit and photoperiod control unit press the instruction of control module, pass through control
In red-light LED light belt, green light LED light belt and blue-ray LED light belt where culture bottle processed at bottle hole the amount of luminescence of LED chip and
Luminous intensity, to control the percentage of all kinds of color of light in the received light of culture bottle, and control lamp plate to culture bottle
Intensity of illumination and light application time.
The light-emitting surface of the LED chip is towards bottle hole axis.
The sterilization unit is uv disinfection unit.
Described set is equipped with induction balance at bottle slot.
Lamp plate lifting module include vertically disposed spindle gear and vertically to linear guide.
The lamp plate is in rectangle, and one jiao of the spindle gear and rectangle lamp plate is connected to drive lamp plate to go up and down, institute
The other three angle for stating rectangle lamp plate, which is placed at linear guide, to be slid.
The lamp plate is connected with fixing clamp with spindle gear, linear guide, and linear guide is equipped with installation fixing clamp
Sliding block.
The lamp plate main body is formed with aluminum metal, and lamp plate is contacted with the LED light source side at lamp plate bottle hole to export LED light
Heat when source works;A bottle slot of setting in the culture bottle fixed plate includes inner ring and outer ring, sets a bottle slot inner ring size adjustable.
In the present invention, when culturing rack component carries out culture operation, lamp plate lifting module drive lamp plate movement makes lamp plate bottle
Hole is placed at taper culture bottle, and the LED light source at lamp plate bottle hole provides illumination to culture bottle;Since culture bottle is carrying out culture work
When industry, bottleneck is equipped with sealing more, this of the invention can be designed so that lamp plate is directly irradiated in light the content of culture bottle
On object, the sealing polybag shielding light of bottle product is avoided to shine.
In the present invention, the culturing rack component includes rack, culture bottle fixed plate, lamp plate, lamp plate lifting module and shake
Module, the culture bottle fixed plate are horizontally placed in rack, and culture bottle fixed plate is equipped with for fixing taper culture bottle
Set a bottle slot;It is connected at the rack that the lamp plate level is set to above culture bottle fixed plate and with lamp plate lifting module;Due to culture
Frame is affixed on agitating device when cultivating operation, which enables lamp plate is synchronous with culturing rack to shake, and solves training
Support the bottle problem uneven because of light intensity variation caused by evolution.
In the present invention, when culturing rack component carries out culture operation, the LED light source at lamp plate bottle hole provides light to culture bottle
According to there are the gaps for forming heat dissipation wind path between lamp plate bottle hole and taper culture bottle outer wall;The temperature-controlling system unit includes warming
The air-out direction of module, radiator fan, exhaust fan and heating element, radiator fan is directed toward culturing rack component, the outlet air of exhaust fan
It is directed toward outside cabinet in direction;The design so that LED light source work when heat will not be directly conducted on culture bottle, will not be to culture
Bottle culture operation impact, and radiator fan to culture bottle fixed plate dry when, cold airflow by lamp plate bottle hole and cone
Gap between shape culture bottle outer wall can play heat-blocking action, so that LED light source heat is not easy to influence the temperature environment of culture bottle.
In the present invention, the lamp plate main body is formed with aluminum metal, lamp plate contacted with the LED light source side at lamp plate bottle hole with
Heat when LED light source work is exported, the air-out direction of radiator fan is directed toward culturing rack component, and the air-out direction of exhaust fan is directed toward
Outside cabinet;It is connected at the rack that the lamp plate level is set to above culture bottle fixed plate and with lamp plate lifting module;The design shape
At the large area heat radiating fin structure at lamp plate bottle hole, the wind energy that goes out of radiator fan blows on lamp plate and takes away the heat on lamp plate,
So that the heat of LED light source can be drained into soon outside incubator, it is not easy to impact the temperature environment in incubator.
In the present invention, the illuminator of multiple LED light sources at the inner wall of the bottle hole is LED chip, and the LED chip includes
Red LED chip, green LED chip and blue-light LED chip, multiple LED light sources are by photochromic different in bottle hole inner wall punishment group row
Column form red-light LED light belt, green light LED light belt and blue-ray LED light belt;The light quality control unit, intensity control unit and light
Period control unit presses the instruction of control module, passes through red-light LED light belt, the green light LED light where control culture bottle at bottle hole
The amount of luminescence and luminous intensity of LED chip in band and blue-ray LED light belt, to control all kinds of face in the received light of culture bottle
The percentage of coloured light, and control lamp plate is to the intensity of illumination and light application time of culture bottle;The design enables during lamp plate issues
The feux rouges of a length of 660nm of cardiac wave;Central wavelength is the green-yellow light of 520nm;Central wavelength is the blue light of 450nm, and the photoperiod is realized
The light application time of each color LED chip is distinguished adjustable in 0-24h, meets suspension culture in different stages of growth to not sharing the same light
According to intensity, the demand of different light medium and different light application times, and allow lamp plate to the intensity of illumination of culture bottle, the content of white light
It can accurately control, be conducive to incubator and various organization is cultivated using corresponding luminous environment, promote the compatibility of incubator.
In the present invention, bottle slot of setting in the culture bottle fixed plate includes inner ring and outer ring, sets a bottle slot inner ring size adjustable;
The design can be suitably used for the standard conical flask that capacity is 150ml, 200ml, 250ml so that setting bottle slot, greatly improve this product
Compatibility.
In the present invention, described set is equipped with induction balance at bottle slot;The design, which uses, can incude culturing rack component states
Weighing equipment understands in real time so that system can transfer the quality of any conical flask when the rack of culturing rack component stops swinging
Suspension cell growth state, provides for user compared with convenience.
In the present invention, the lamp plate is connected with fixing clamp with spindle gear, linear guide, and linear guide is equipped with peace
Fill the sliding block of fixing clamp;The design is so that the lamp plate of this product is readily disassembled.
In the present invention, cabinet is equipped with glass window;The luminous environment control unit includes light quality control unit, photoperiod
Control unit, intensity control unit, light excluding shutter and antiglare module, the light excluding shutter be set to glass window at and with antiglare module phase
Even;This is designed as this product and provides half-light function, since some plant cells are suitable for dark culture, when this product is for such thin
When the culture of born of the same parents, need to only it allow antiglare module that light excluding shutter is put down.
Detailed description of the invention
The present invention is described in more detail with reference to the accompanying drawings and detailed description:
Attached drawing 1 is the schematic diagram of culturing rack component of the present invention;
Attached drawing 2 is the schematic diagram of lamp plate of the present invention;
Attached drawing 3 is the schematic diagram of the linear guide of lamp plate lifting module of the present invention;
Attached drawing 4 is the schematic diagram of the spindle gear of lamp plate lifting module of the present invention;
Attached drawing 5 is culturing rack component of the present invention showing when not installing lamp plate, light source lifting device, inner ring telescopic mounting
It is intended to;
Attached drawing 6 is the schematic diagram for the taper culture bottle that the present invention refers to;
Attached drawing 7 is the outer ring that bottle slot is set in the present invention, the schematic diagram of inner ring;
Attached drawing 8 is the schematic diagram of incubator of the present invention;
Attached drawing 9 is the schematic diagram of incubator chamber door of the present invention;
Attached drawing 10 is the connection schematic diagram of control module of the invention;
Attached drawing 11 is the schematic diagram of culture bottle fixed plate of the invention;
In figure: 1- taper culture bottle;2-LED light source;3- lamp plate;Bottle hole on 4- lamp plate;5- linear guide;6- sliding block;
7- fixing clamp;8- stepper motor;9- shaft coupling;10- spindle gear;11- screw rod through-hole;12- applanation thrust ball axis
It holds;The 13- support of bearing;14- sets a bottle slot;15- sets a bottle slot outer ring;16- sets inner ring of bottle slot with telescopic mounting;17- cabinet;
18- culturing rack component;19- chamber door;20- light excluding shutter;21- glass window;22- control module;23- shakes module;24- lamp plate liter
Module drops;25- temperature-controlling system unit 26- Weighing system unit;27- sterilization unit;28- photoperiod control unit;29- light quality
Control unit;30- intensity control unit;31- incudes balance;32- culture bottle fixed plate;33- rack.
Specific embodiment
As shown in figs. 1-11, a kind of high-light-energy utilization rate shaking table culture case for the culture that suspends, the incubator include
Cabinet 17, culturing rack component 18, sterilization unit 27, temperature-controlling system unit 25, luminous environment control unit and control module 22, it is described
Control module 22 is connected with sterilization unit 27, temperature-controlling system unit 25 and luminous environment control unit, and cabinet 17 is equipped with windowpane
Mouth 21;The culturing rack component includes rack, culture bottle fixed plate 32, lamp plate 3, lamp plate lifting module 24 and shakes module 23,
The culture bottle fixed plate 32 is horizontally placed in rack 33, and culture bottle fixed plate 32 is equipped with for fixing taper culture bottle 1
Set bottle slot 14;24 phase of module is gone up and down at the horizontal rack 33 for being set to 32 top of culture bottle fixed plate of lamp plate 3 and with lamp plate
Even, lamp plate 3 is equipped with multiple bottle holes 4 probeed into for 1 top of taper culture bottle, is uniformly provided at the inner wall in each bottle of hole 4 multiple
LED light source 2;The luminous environment control unit includes light quality control unit, photoperiod control unit, intensity control unit, shading
Door and antiglare module, the light quality control unit 29, intensity control unit 30 are connected with the LED light source 2 on lamp plate 3, the screening
Optical gate 20 is set at glass window 21 and is connected with antiglare module;The temperature-controlling system unit 25 includes warming module, radiation air
Fan, exhaust fan and heating element, the air-out direction of radiator fan are directed toward culturing rack component 18, and the air-out direction of exhaust fan is directed toward case
Outside body 17, when the temperature inside the box that warming module measures exceeds threshold value, temperature-controlling system module 25 controls the temperature inside the box;When
When culturing rack component 18 carries out culture operation, shakes module 23 and rack 33 is driven to shake, it is mobile that lamp plate goes up and down module drive lamp plate 3
It is placed on 3 bottles of holes 4 of lamp plate at taper culture bottle 1, the LED light source 2 at lamp plate bottle hole 4 provides illumination, lamp plate bottle hole to culture bottle 1
There are the gaps for forming heat dissipation wind path between 4 and 1 outer wall of taper culture bottle.
The illuminator of multiple LED light sources 2 at the inner wall of the bottle hole is LED chip, and the LED chip includes red-light LED
Chip, green LED chip and blue-light LED chip, multiple LED light sources are by photochromic different in the arrangement of bottle hole inner wall punishment group, formation
Red-light LED light belt, green light LED light belt and blue-ray LED light belt.
The light quality control unit 29, intensity control unit 30 and photoperiod control unit 28 press the finger of control module 22
It enables, passes through LED chip in red-light LED light belt where control culture bottle at bottle hole, green light LED light belt and blue-ray LED light belt
Amount of luminescence and luminous intensity, to control the percentage of all kinds of color of light in the received light of culture bottle, and control lamp plate
To the intensity of illumination and light application time of culture bottle.
The light-emitting surface of the LED chip is towards bottle hole axis.
The sterilization unit 27 is uv disinfection unit.
Described set is equipped with induction balance 31 at bottle slot 14.
Lamp plate lifting module 24 include vertically disposed spindle gear 10 and vertically to linear guide 5.
The lamp plate 3 is in rectangle, and one jiao of the spindle gear 10 and rectangle lamp plate 3 is connected to drive 3 liters of lamp plate
Drop, the other three angle of the rectangle lamp plate 3 are placed at linear guide 5 and slide.
The lamp plate 3 is connected with fixing clamp 7 with spindle gear 10, linear guide 5, and linear guide 5 is equipped with installation
The sliding block 6 of fixing clamp 7.
3 main body of lamp plate is formed with aluminum metal, and lamp plate 3 is contacted with the LED light source side at lamp plate bottle hole to export LED
Heat when light source working;Bottle slot 14 of setting in the culture bottle fixed plate 32 includes inner ring 16 and outer ring 15, sets a bottle slot inner ring
16 size adjustables.
Embodiment:
Bottle bulk of setting that 14 inner ring of bottle slot is set in operator's adjusting is coincide up to taper culture bottle 1, then cone
Shape culture bottle 1, which is put in, to be set in bottle slot 14;The culturing rack component 18 for being equipped with taper culture bottle 1 is fixed on and is shaken in module 23, is opened
Spindle gear 10 at dynamic installation screw rod through-hole 11, applanation thrust ball bearing 12 and the support of bearing 13, stepper motor 8
Power is provided to spindle gear by shaft coupling 9, driving lamp plate 3 vertically moves, until the bottle hole 4 on lamp plate 3 is placed on cone
The body of shape culture bottle 1, there are the gaps for forming heat dissipation wind path between 1 outer wall of lamp plate bottle hole 4 and taper culture bottle.
Control module controls LED light source 2 through light quality control unit 29, intensity control unit 30 and photoperiod control unit 28
Shine the illumination for providing to taper culture bottle 1 and being suitble to content culture in bottle;Heat when LED light source works is rapidly transferred to aluminium
It on lamp plate processed, is radiated by the radiator fan of temperature-controlling system unit 25 to lamp plate, the exhaust fan of temperature-controlling system unit 25 is training
Support the hot-air discharge in case;Operator, which starts, shakes module 23, shakes module 23 and drives rack 33, culture bottle fixed plate 32
It is synchronous with lamp plate 3 to shake, start training.
Shaking module periodically drives culturing rack component 18 to shake, and when culturing rack component 18 stops shaking, incudes day
The flat current weight of 31 automatic weighing conical flasks simultaneously reaches data at control module, carries out corresponding data exhibition by control module
Show, data-storing and data transmission and processing.