CN106479892A - A kind of high-light-energy utilization rate shaking table culture case for suspension culture - Google Patents
A kind of high-light-energy utilization rate shaking table culture case for suspension culture Download PDFInfo
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Abstract
The present invention proposes a kind of high-light-energy utilization rate shaking table culture case for suspension culture, including casing, culturing rack assembly, sterilization unit, temperature-controlling system unit, luminous environment control unit and control module, culturing rack assembly includes frame, culture bottle fixed plate, lamp plate, lamp plate lifting module and shake module, and culture bottle fixed plate is provided with for putting a bottle groove；At frame above culture bottle fixed plate for the lamp plate level and lamp plate is provided with a bottle hole, bottle hole is provided with LED light source；The radiator fan air-out direction of temperature-controlling system unit points to culturing rack assembly, and the air-out direction of exhaust fan points to outside casing；When culturing rack assembly carries out cultivating operation, shake module drive frame shake, lamp plate lifting module drive lamp plate movement makes lamp plate bottle borehole jack at taper culture bottle, and LED light source provides illumination to culture bottle, leaves the gap forming radiating wind path between lamp plate bottle hole and culture bottle outer wall；The present invention can provide customizable photoenvironment with LED light source, and is difficult to affect case temperature because of heat of light source.
Description
Technical field
The present invention relates to being related to optics culture device, especially a kind of high-light-energy utilization rate shaking table training for suspension culture
Foster case.
Background technology
Suspension culture refer to a kind of constantly stirred or the fluid medium that shakes in, culture is unicellular and little thin
The tissue culture system of born of the same parents group, is a kind of training method of non-anchorage-dependent cell.Suspension culture is in secondary metabolite
It is widely used in production, and there is unrivaled superiority.Under normal circumstances, the standard taper of suspension culture used by laboratory
The capacity of bottle is 150ml, 200ml, 250ml, and body external diameter is respectively 69mm, 75mm, 85mm.Nowadays constant-temperature table is suspending
The utilization of culture is more and more extensive, but when studying the impact to suspension culture for the light, a lot of constant-temperature tables all reach on the market
Less than requirement of experiment.Light can improve quality and its secondary metabolites content of suspension culture, but shaking table on the market, lamp source
The problems such as sealing plastic bag of the unreasonable and conical flask of mounting design shuts out the light, badly influences the uniformity of illumination,
Experimental result is made to there is very big error.Therefore research and develop a high-light-energy utilization rate shaking table culture frame just very necessary.
Lamp source design is the core of high-light-energy utilization rate shaking table.Due to suspension culture particularity：Taper bottle closure is moulded
Pocket and conical flask are constantly being shifted one's position, and all can have influence on the uniformity of illumination.Constant-temperature table on the market at present, lamp source
Mounting design multifarious, all can not meet illumination cultivation.Lamp bar is installed on side by some shaking tables, and some shaking tables are by lamp plate
It is installed on top, but be unable to solve the problems, such as optical uniformity；The lamp with more excellent photoenvironment how is designed for suspension culture
Source, is a research direction.
LED light source is a kind of cold light source in recent years rising, and has the advantages that economize on electricity and save space, partial monopoly is LED
Light source is used for incubator, and the light-emitting area of LED light source is directional and light quantity is limited, need to have the common work of a number of LED chip
Make just easily to reach luminous environment needed for incubator, though but LED light source is cold light source has certain operating temperature, such as in culture
There is a possibility that the temperature environment of incubator is wayward using LED light source in a large number in case, how to solve these problems, be also one
Research direction.
Content of the invention
The present invention proposes a kind of high-light-energy utilization rate shaking table culture case for suspension culture, and can be provided with LED light source can
The photoenvironment of customization, and be difficult to affect case temperature because of heat of light source.
The present invention employs the following technical solutions.
A kind of high-light-energy utilization rate shaking table culture case for suspension culture, described incubator includes casing, culturing rack group
Part, sterilization unit, temperature-controlling system unit, luminous environment control unit and control module, described control module and sterilization unit, temperature control
System unit is connected with luminous environment control unit, and casing is provided with glass window；Described culturing rack assembly includes frame, culture bottle
Fixed plate, lamp plate, lamp plate lifting module and shake module, described culture bottle fixed plate is horizontally placed in frame, and culture bottle is solid
Fixed board is provided with and puts a bottle groove for fix taper culture bottle；At frame above culture bottle fixed plate for the described lamp plate level
And be connected with lamp plate lifting module, lamp plate is provided with the bottle hole that multiple confession taper culture bottle tops probe into, the inwall in each bottle of hole
Place is uniformly provided with multiple LED light sources；Described luminous environment control unit includes light quality control unit, photoperiod control unit, light intensity
Control unit, light excluding shutter and antiglare module, described light quality control unit, intensity control unit are connected with the LED light source on lamp plate,
Described light excluding shutter is connected at glass window and with antiglare module；Described temperature-controlling system unit includes warming module, radiation air
Fan, exhaust fan and heating, the air-out direction of radiator fan points to culturing rack assembly, and the air-out direction of exhaust fan points to casing
Outward, when the temperature inside the box that warming module records exceeds threshold value, temperature-controlling system module is controlled to the temperature inside the box；Work as culturing rack
When assembly carries out cultivating operation, shake module drive frame shake, lamp plate lifting module drive lamp plate movement makes lamp plate bottle borehole jack
At taper culture bottle, the LED light source at lamp plate bottle hole provides illumination to culture bottle, between lamp plate bottle hole and taper culture bottle outer wall
Leave the gap forming radiating wind path.
The luminous body of the multiple LED light sources at described bottle in the hole wall is LED chip, and described LED chip includes red-light LED core
Piece, green LED chip and blue-light LED chip, multiple LED light sources press photochromic different packet arrangement at bottle in the hole wall, are formed red
Light LED light strip, green light LED light belt and blue-ray LED light belt.
Described light quality control unit, intensity control unit and photoperiod control unit press the instruction of control module, by control
In red-light LED light belt at the bottle hole of culture bottle place processed, green light LED light belt and blue-ray LED light belt the amount of luminescence of LED chip and
Luminous intensity, to control the percentage ratio of all kinds of color of light in the accepted light of culture bottle, and controls lamp plate to culture bottle
Intensity of illumination and light application time.
The light-emitting area of described LED chip is towards bottle hole axis.
Described sterilization unit is uv disinfection unit.
Described putting is provided with sensing balance at bottle groove.
Described lamp plate lift module include vertically disposed spindle gear and vertical to line slideway.
Described lamp plate is rectangular, and one jiao of described spindle gear and rectangle lamp plate is connected to drive lamp plate lifting, institute
The other three angle stating rectangle lamp plate is placed in sliding at line slideway.
Described lamp plate is connected with spindle gear, line slideway with fixation clamp, and line slideway is provided with installation fixation clamp
Slide block.
, with aluminum metal molding, the LED light source contacts side surfaces at lamp plate and lamp plate bottle hole are to derive LED light for described lamp plate main body
The heat during work of source；A bottle groove of putting in described culture bottle fixed plate includes inner ring and outer ring, puts a bottle groove inner ring size adjustable.
In the present invention, when culturing rack assembly carries out cultivating operation, lamp plate lifting module drive lamp plate movement makes lamp plate bottle
At taper culture bottle, the LED light source at lamp plate bottle hole provides illumination to culture bottle to borehole jack；Because culture bottle is carrying out culture work
During industry, bottleneck, being provided with sealing, this of the present invention can be designed so that lamp plate is directly irradiated in light the content of culture bottle more
On thing, it is to avoid the sealing plastic bag of bottle product blocks illumination.
In the present invention, described culturing rack assembly includes frame, culture bottle fixed plate, lamp plate, lamp plate lifting module and shake
Module, described culture bottle fixed plate is horizontally placed in frame, and culture bottle fixed plate is provided with for fixing taper culture bottle
Put a bottle groove；It is connected at frame above culture bottle fixed plate for the described lamp plate level and with lamp plate lifting module；Due to culture
Frame is affixed on agitating device when cultivating operation, and this design enables lamp plate synchronously to shake with culturing rack, solves training
The problem of the light intensity change inequality that foster bottle leads to because of evolution.
In the present invention, when culturing rack assembly carries out cultivating operation, the LED light source at lamp plate bottle hole provides light to culture bottle
According to, leave between lamp plate bottle hole and taper culture bottle outer wall formed radiating wind path gap；Described temperature-controlling system unit includes warming
Module, radiator fan, exhaust fan and heating, the air-out direction of radiator fan points to culturing rack assembly, the air-out of exhaust fan
Direction is pointed to outside casing；This design makes heat during LED light source work will not be directly conducted on culture bottle, will not be to culture
The culture operation of bottle impacts, and when radiator fan is dried to culture bottle fixed plate, cold airflow passes through lamp plate bottle hole and cone
Gap between shape culture bottle outer wall can play heat-blocking action so that LED light source heat is difficult to affect the temperature environment of culture bottle.
In the present invention, described lamp plate main body with aluminum metal molding, LED light source contacts side surfaces at lamp plate and lamp plate bottle hole with
Derive heat during LED light source work, the air-out direction of radiator fan points to culturing rack assembly, and the air-out direction of exhaust fan points to
Outside casing；It is connected at frame above culture bottle fixed plate for the described lamp plate level and with lamp plate lifting module；This design shape
Become the large area heat radiating fin structure at lamp plate bottle hole, radiator fan go out the heat that wind energy blows on lamp plate and takes away on lamp plate,
So that the heat of LED light source can soon drain into outside incubator, it is difficult the temperature environment in incubator is impacted.
In the present invention, the luminous body of the multiple LED light sources at described bottle in the hole wall is LED chip, and described LED chip includes
Red LED chip, green LED chip and blue-light LED chip, multiple LED light sources press photochromic different packet row at bottle in the hole wall
Row, form red-light LED light belt, green light LED light belt and blue-ray LED light belt；Described light quality control unit, intensity control unit and light
Periodic Control unit presses the instruction of control module, by the red-light LED light belt at control culture bottle place bottle hole, green light LED light
In band and blue-ray LED light belt, the amount of luminescence of LED chip and luminous intensity, to control all kinds of face in the accepted light of culture bottle
The percentage ratio of coloured light, and control lamp plate to the intensity of illumination of culture bottle and light application time；During this design enables lamp plate to send
The HONGGUANG of a length of 660nm of cardiac wave；Centre wavelength is the green-yellow light of 520nm；Centre wavelength is the blue light of 450nm, and the photoperiod is realized
The light application time of each color LED chip is respectively adjustable in 0-24h, meets suspension culture in different growth phases to not sharing the same light
According to the demand of intensity, different light medium and different light application time, and allow the intensity of illumination to culture bottle for the lamp plate, the content of white light
Energy precise control, is conducive to incubator that various organization is cultivated using corresponding luminous environment, the compatibility of lifting incubator.
In the present invention, bottle groove of putting in described culture bottle fixed plate includes inner ring and outer ring, puts a bottle groove inner ring size adjustable；
This design makes to put bottle groove and can be suitably used for the standard conical flask that capacity is 150ml, 200ml, 250ml, greatly improves this product
Compatibility.
In the present invention, described putting is provided with sensing balance at bottle groove；This has been designed with sensing culturing rack component states
Weighing equipment, so that when the frame of culturing rack assembly stops swinging, system adjustable takes the quality of any conical flask, understands in real time
Suspension cell growth state, is that user provides compared with convenience.
In the present invention, described lamp plate is connected with spindle gear, line slideway with fixation clamp, and line slideway is provided with peace
The slide block of dress fixation clamp；This design makes the lamp plate of this product be readily disassembled.
In the present invention, casing is provided with glass window；Described luminous environment control unit includes light quality control unit, photoperiod
Control unit, intensity control unit, light excluding shutter and antiglare module, described light excluding shutter at glass window and with antiglare module phase
Even；This is designed as this product and provides half-light function, because some plant cells are suitable to light culture, when this product is for such thin
During the culture of born of the same parents, only need to allow antiglare module that light excluding shutter is put down.
Brief description
The present invention is described in more detail with reference to the accompanying drawings and detailed description：
Accompanying drawing 1 is the schematic diagram of culturing rack assembly of the present invention；
Accompanying drawing 2 is the schematic diagram of lamp plate of the present invention；
Accompanying drawing 3 is the schematic diagram of the line slideway that lamp plate of the present invention lifts module；
Accompanying drawing 4 is the schematic diagram of the spindle gear that lamp plate of the present invention lifts module；
Accompanying drawing 5 is the signal when not installing lamp plate, light source lowering or hoisting gear, inner ring telescopic mounting for the culturing rack assembly of the present invention
Figure；
Accompanying drawing 6 is the schematic diagram of the taper culture bottle that the present invention refers to；
Accompanying drawing 7 is outer ring, the schematic diagram of inner ring putting bottle groove in the present invention；
Accompanying drawing 8 is the schematic diagram of incubator of the present invention；
Accompanying drawing 9 is the schematic diagram of incubator chamber door of the present invention；
Accompanying drawing 10 is the connection diagram of the control module of the present invention；
Accompanying drawing 11 is the schematic diagram of the culture bottle fixed plate of the present invention；
In figure：1- taper culture bottle；2-LED light source；3- lamp plate；Bottle hole on 4- lamp plate；5- line slideway；6- slide block；7- is solid
Clamp；8- motor；9- shaft coupling；10- spindle gear；11- screw mandrel through hole；12- applanation thrust ball bearing；
The 13- support of bearing；14- puts a bottle groove；15- puts a bottle groove outer ring；16- puts the inner ring with telescopic mounting for bottle groove；17- casing；18- trains
Foster frame assembly；19- chamber door；20- light excluding shutter；21- glass window；22- control module；23- shakes module；24- lamp plate lifts mould
Block；25- temperature-controlling system unit 26- Weighing system unit；27- sterilization unit；28- photoperiod control unit；29- light quality controls
Unit；30- intensity control unit；31- senses balance；32- culture bottle fixed plate；33- frame.
Specific embodiment
As shown in figs. 1-11, a kind of high-light-energy utilization rate shaking table culture case for suspension culture, described incubator includes
Casing 17, culturing rack assembly 18, sterilization unit 27, temperature-controlling system unit 25, luminous environment control unit and control module 22, described
Control module 22 is connected with sterilization unit 27, temperature-controlling system unit 25 and luminous environment control unit, and casing 17 is provided with windowpane
Mouth 21；Described culturing rack assembly includes frame, culture bottle fixed plate 32, lamp plate 3, lamp plate lifting module 24 and shake module 23,
Described culture bottle fixed plate 32 is horizontally placed in frame 33, and culture bottle fixed plate 32 is provided with for fixing taper culture bottle 1
Put bottle groove 14；Described lamp plate 3 level lifts module 24 phase at the frame 33 of culture bottle fixed plate 32 top and with lamp plate
Even, lamp plate 3 is provided with the bottle hole 4 that multiple confession taper culture bottle 1 tops probe into, is uniformly provided with multiple at the inwall in each bottle of hole 4
LED light source 2；Described luminous environment control unit includes light quality control unit, photoperiod control unit, intensity control unit, shading
Door and antiglare module, described light quality control unit 29, intensity control unit 30 are connected with the LED light source 2 on lamp plate 3, described screening
Optical gate 20 is connected at glass window 21 and with antiglare module；Described temperature-controlling system unit 25 includes warming module, radiation air
Fan, exhaust fan and heating, the air-out direction of radiator fan points to culturing rack assembly 18, and the air-out direction of exhaust fan points to case
Outside body 17, when the temperature inside the box that warming module records exceeds threshold value, temperature-controlling system module 25 is controlled to the temperature inside the box；When
When culturing rack assembly 18 carries out cultivating operation, shake module 23 drives frame 33 to shake, and lamp plate lifting module drive lamp plate 3 is mobile
3 bottles of holes 4 of lamp plate are made to be placed at taper culture bottle 1, the LED light source 2 at lamp plate bottle hole 4 provides illumination, lamp plate bottle hole to culture bottle 1
The gap forming radiating wind path is left between 4 and taper culture bottle 1 outer wall.
The luminous body of the multiple LED light sources 2 at described bottle in the hole wall is LED chip, and described LED chip includes red-light LED
Chip, green LED chip and blue-light LED chip, multiple LED light sources press photochromic different packet arrangement at bottle in the hole wall, are formed
Red-light LED light belt, green light LED light belt and blue-ray LED light belt.
Described light quality control unit 29, intensity control unit 30 and photoperiod control unit 28 press the finger of control module 22
Order, by LED chip in the red-light LED light belt at control culture bottle place bottle hole, green light LED light belt and blue-ray LED light belt
Amount of luminescence and luminous intensity, to control the percentage ratio of all kinds of color of light in the accepted light of culture bottle, and control lamp plate
Intensity of illumination to culture bottle and light application time.
The light-emitting area of described LED chip is towards bottle hole axis.
Described sterilization unit 27 is uv disinfection unit.
Described putting is provided with sensing balance 31 at bottle groove 14.
Described lamp plate lift module 24 include vertically disposed spindle gear 10 and vertical to line slideway 5.
Described lamp plate 3 is rectangular, and one jiao of described spindle gear 10 and rectangle lamp plate 3 is connected to drive 3 liters of lamp plate
Fall, the other three angle of described rectangle lamp plate 3 is placed in sliding at line slideway 5.
Described lamp plate 3 is connected with spindle gear 10, line slideway 5 with fixation clamp 7, and line slideway 5 is provided with installation
The slide block 6 of fixation clamp 7.
, with aluminum metal molding, the LED light source contacts side surfaces at lamp plate 3 and lamp plate bottle hole are to derive LED for described lamp plate 3 main body
Heat during light source works；Bottle groove 14 of putting in described culture bottle fixed plate 32 includes inner ring 16 and outer ring 15, puts a bottle groove inner ring
16 size adjustable.
Embodiment：
Bottle bulk of putting that bottle groove 14 inner ring is put in operator's regulation is coincide up to taper culture bottle 1, then taper is trained
Foster bottle 1 is put in be put in bottle groove 14；The culturing rack assembly 18 being equipped with taper culture bottle 1 is fixed in shake module 23, starts peace
Spindle gear 10 at dress screw mandrel through hole 11, applanation thrust ball bearing 12 and the support of bearing 13, motor 8 passes through
Shaft coupling 9 provides power to spindle gear, drives lamp plate 3 to vertically move, until the bottle hole 4 on lamp plate 3 is placed on taper training
The body of foster bottle 1, leaves the gap forming radiating wind path between lamp plate bottle hole 4 and taper culture bottle 1 outer wall.
Control module controls LED light source 2 through light quality control unit 29, intensity control unit 30 and photoperiod control unit 28
Light and the illumination being suitable for content culture in bottle is provided to taper culture bottle 1；Heat during LED light source work is rapidly transferred to aluminum
On lamp plate processed, by the radiator fan of temperature-controlling system unit 25, lamp plate is radiated, the exhaust fan of temperature-controlling system unit 25 is training
Hot-air in foster case is discharged；Operator start shake module 23, and shake module 23 drives frame 33, culture bottle fixed plate 32
Synchronous shake, starts training with lamp plate 3.
Shake module periodically drives culturing rack assembly 18 to shake, and when culturing rack assembly 18 stops shake, senses sky
The current weight of flat 31 automatic weighing conical flasks simultaneously reaches data at control module, carries out corresponding data exhibition by control module
Show, data-storing data transmission process.
Claims (10)
1. a kind of high-light-energy utilization rate shaking table culture case for suspension culture it is characterised in that：Described incubator include casing,
Culturing rack assembly, sterilization unit, temperature-controlling system unit, luminous environment control unit and control module, described control module and sterilizing
Unit, temperature-controlling system unit are connected with luminous environment control unit, and casing is provided with glass window；Described culturing rack assembly includes machine
Frame, culture bottle fixed plate, lamp plate, lamp plate lifting module and shake module, described culture bottle fixed plate is horizontally placed in frame,
Culture bottle fixed plate is provided with and puts a bottle groove for fix taper culture bottle；Described lamp plate level is above culture bottle fixed plate
Frame at and with lamp plate lifting module be connected, lamp plate is provided with the bottle hole that multiple confession taper culture bottle tops probe into, each bottle
It is uniformly provided with multiple LED light sources at the inwall in hole；Described luminous environment control unit includes light quality control unit, the photoperiod controls list
Unit, intensity control unit, light excluding shutter and antiglare module, the LED light on described light quality control unit, intensity control unit and lamp plate
Source is connected, and described light excluding shutter is connected at glass window and with antiglare module；Described temperature-controlling system unit include warming module,
Radiator fan, exhaust fan and heating, the air-out direction of radiator fan points to culturing rack assembly, and the air-out direction of exhaust fan refers to
To outside casing, when the temperature inside the box that warming module records exceeds threshold value, temperature-controlling system module is controlled to the temperature inside the box；When
When culturing rack assembly carries out cultivating operation, shake module drive frame shake, lamp plate lifting module drive lamp plate movement makes lamp plate
At taper culture bottle, the LED light source at lamp plate bottle hole provides illumination, lamp plate bottle hole and taper culture bottle to bottle borehole jack to culture bottle
The gap forming radiating wind path is left between outer wall.
2. a kind of high-light-energy utilization rate shaking table culture case for suspension culture according to claim 1 it is characterised in that：
The luminous body of the multiple LED light sources at described bottle in the hole wall is LED chip, and described LED chip includes red LED chip, green glow
LED chip and blue-light LED chip, multiple LED light sources press photochromic different packet arrangement at bottle in the hole wall, form red-light LED light
Band, green light LED light belt and blue-ray LED light belt.
3. a kind of high-light-energy utilization rate shaking table culture case for suspension culture according to claim 2 it is characterised in that：
Described light quality control unit, intensity control unit and photoperiod control unit press the instruction of control module, by controlling culture bottle
The amount of luminescence of LED chip and luminous strong in red-light LED light belt at the bottle hole of place, green light LED light belt and blue-ray LED light belt
Degree, to control the percentage ratio of all kinds of color of light in the accepted light of culture bottle, and controls lamp plate strong to the illumination of culture bottle
Degree and light application time.
4. a kind of high-light-energy utilization rate shaking table culture case for suspension culture according to claim 3 it is characterised in that：
The light-emitting area of described LED chip is towards bottle hole axis.
5. a kind of high-light-energy utilization rate shaking table culture case for suspension culture according to claim 1 it is characterised in that：
Described sterilization unit is uv disinfection unit.
6. a kind of high-light-energy utilization rate shaking table culture case for suspension culture according to claim 1 it is characterised in that：
Described putting is provided with sensing balance at bottle groove.
7. a kind of high-light-energy utilization rate shaking table culture case for suspension culture according to claim 1 it is characterised in that：
Described lamp plate lift module include vertically disposed spindle gear and vertical to line slideway.
8. a kind of high-light-energy utilization rate shaking table culture case for suspension culture according to claim 7 it is characterised in that：
Described lamp plate is rectangular, and one jiao of described spindle gear and rectangle lamp plate is connected to drive lamp plate lifting, described rectangular lamps
The other three angle of plate is placed in sliding at line slideway.
9. a kind of high-light-energy utilization rate shaking table culture case for suspension culture according to claim 8 it is characterised in that：
Described lamp plate is connected with spindle gear, line slideway with fixation clamp, and line slideway is provided with the slide block installing fixation clamp.
10. a kind of high-light-energy utilization rate shaking table culture case for suspension culture according to claim 1, its feature exists
In：, with aluminum metal molding, the LED light source contacts side surfaces at lamp plate and lamp plate bottle hole are to derive LED light source work for described lamp plate main body
Heat when making；A bottle groove of putting in described culture bottle fixed plate includes inner ring and outer ring, puts a bottle groove inner ring size adjustable.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107853180A (en) * | 2017-12-05 | 2018-03-30 | 福建农林大学 | The tissue culture device and its cultural method of integral type controllable light |
| CN108641915A (en) * | 2018-06-06 | 2018-10-12 | 佛山科学技术学院 | A kind of body structure suitable for shaking flask culture |
| CN108795761A (en) * | 2018-07-11 | 2018-11-13 | 北京鼎翰科技有限公司 | One kind being based on bipolar slice cell culture apparatus |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050084420A1 (en) * | 2003-10-20 | 2005-04-21 | Sanyo Electric Co., Ltd. | Incubator |
| CN201420079Y (en) * | 2009-05-19 | 2010-03-10 | 嘉兴市中新医疗仪器有限公司 | Mould incubator |
| CN202595126U (en) * | 2012-05-22 | 2012-12-12 | 中国水产科学研究院东海水产研究所 | Primary culture device of microalgae |
| CN204598758U (en) * | 2015-04-11 | 2015-09-02 | 福建农林大学 | Plant cultivation frame height adjustable light supply apparatus |
| CN205351006U (en) * | 2016-01-14 | 2016-06-29 | 广明源光科技股份有限公司 | Prop up posture plant tissue culture lamp and plant tissue culture device |
-
2016
- 2016-10-21 CN CN201610918799.1A patent/CN106479892B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20050084420A1 (en) * | 2003-10-20 | 2005-04-21 | Sanyo Electric Co., Ltd. | Incubator |
| CN201420079Y (en) * | 2009-05-19 | 2010-03-10 | 嘉兴市中新医疗仪器有限公司 | Mould incubator |
| CN202595126U (en) * | 2012-05-22 | 2012-12-12 | 中国水产科学研究院东海水产研究所 | Primary culture device of microalgae |
| CN204598758U (en) * | 2015-04-11 | 2015-09-02 | 福建农林大学 | Plant cultivation frame height adjustable light supply apparatus |
| CN205351006U (en) * | 2016-01-14 | 2016-06-29 | 广明源光科技股份有限公司 | Prop up posture plant tissue culture lamp and plant tissue culture device |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107853180A (en) * | 2017-12-05 | 2018-03-30 | 福建农林大学 | The tissue culture device and its cultural method of integral type controllable light |
| CN108641915A (en) * | 2018-06-06 | 2018-10-12 | 佛山科学技术学院 | A kind of body structure suitable for shaking flask culture |
| CN108795761A (en) * | 2018-07-11 | 2018-11-13 | 北京鼎翰科技有限公司 | One kind being based on bipolar slice cell culture apparatus |
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