CN107853180A - The tissue culture device and its cultural method of integral type controllable light - Google Patents

Abstract

The invention relates to an integrated light-controllable tissue culture device and a culture method thereof, comprising a culture frame, a bottle groove for placing a culture bottle is arranged on the culture frame, a bottle cap is arranged on the culture bottle, and the bottle cap LED lamps of different colors are arranged on the lower side, and a light-transmitting diaphragm for isolating the bottle cap and the inner cavity of the culture bottle is arranged in the culture bottle, and a vent pipe upwardly passing through the bottle cap is arranged on the light-transmitting diaphragm. The integrated light-controllable tissue culture device of the present invention provides good light conditions for plants that need to grow under different light qualities or single light quality, reduces the time cost of plant tissue culture, improves the utilization rate of light, and improves plant tissue culture. Efficiency and more environmental protection; at the same time, it solves the problems of bottle heat dissipation and unsafe bottle placement, provides a good environment for plant cell tissue culture, improves survival rate, shortens plant cell tissue culture cycle, and reduces costs.

Description

Integrated light-controllable tissue culture device and culture method thereof

technical field

The invention relates to an integrated light-controllable tissue culture device and a culture method thereof, belonging to the field of plant tissue culture devices.

Background technique

Plant tissue culture refers to the technology of separating the required tissues, organs or cells, protoplasts, etc. from the plant body, and cultivating them under artificial control conditions through aseptic operation to obtain regenerated complete plants or produce other products of economic value . With the development of modern agriculture, people continue to strengthen the research and application of plant tissue culture technology. Compared with traditional seed culture, plant tissue culture technology has greatly improved the survival rate of plant culture, solved the problem of preserving seeds to a certain extent, and allowed some endangered species of plants to be continued. In addition, plant tissue culture technology plays a role in promoting the development of social economy, and it provides great convenience for obtaining many precious medicinal materials. In short, plant tissue culture technology has great development prospects, and it is necessary to strengthen research in this field.

Placing culture materials in a suitable environment is one of the most critical aspects of plant tissue culture. Among them, tissue culture bottles are needed, and their function is to provide suitable light, water, nutrition, temperature and other conditions for plant tissues. How to solve the lighting problem is a key point in the design of tissue culture devices. At present, commonly used culture bottles mostly use plastic caps and transparent glass bottles to solve the problem of light. The problem is that after a period of time, the wall of the tissue culture bottle will produce water droplets, which will affect the light transmittance; in addition, the plastic cover of the tissue culture bottle also has the problem of shading, which wastes light sources; another problem is that, Different plants have different requirements for light quality during the growth process. The growth of many plants requires one or more than one different light quality to provide light at the same time. If a reasonable light quality cannot be provided, the growth time of plant tissue will be greatly extended. Even cause plant tissue culture to fail, improve cost.

Contents of the invention

In view of this, the object of the present invention is to provide an integrated light-controllable tissue culture device and a culture method thereof that provide good light conditions for plants, reduce the time cost of plant tissue culture, improve plant tissue culture efficiency, and improve survival. Rate.

The present invention adopts the following schemes to realize: an integrated light-controllable tissue culture device, including a culture rack, a bottle groove for placing a culture bottle is arranged on the culture rack, a bottle cap is arranged on the culture bottle, and the bottle cap LED lights of different colors are arranged on the lower side, and a light-transmitting diaphragm for isolating the bottle cap and the inner cavity of the culture bottle is arranged in the culture bottle, and a ventilating tube upwardly passing through the bottle cap is arranged on the light-transmitting diaphragm.

Further, the bottom of the culture frame is provided with a storage tank located directly below the bottle tank, and the bottom of the bottle tank is provided with air holes communicating with the storage tank, and a micro fan is arranged in the storage tank.

Further, a clamping device for clamping and fixing the culture bottle is arranged on the side of the bottle tank, and a fan switch is arranged on the side of the bottle tank to control the miniature fan on the culture rack.

Further, the bottle cap is composed of an aluminum base plate and a cover body covered on the aluminum base plate, the bottle cap is connected to the culture bottle through threads, the LED light is arranged on the lower side of the aluminum base plate, the aluminum base plate and the cover The body is provided with perforations for the ventilation pipe to pass upwards.

Further, a crossbeam is arranged above the culture frame, a power supply box is arranged on the side of the crossbeam, a junction box is arranged on the lower side of the crossbeam above the bottle cap, and a power line is connected between the junction box and the bottle cap , the bottle cap is provided with an intensity control switch for controlling the intensity of light.

Another technical solution of the present invention: a method for cultivating the above-mentioned integrated light-controllable tissue culture device, comprising the following steps: (1) putting the treated plant tissue into a culture bottle; (2) putting the culture bottle into Put it into the bottle groove of the culture rack, and use the clamping device to fix the culture bottle; (3) Put the light-transmitting diaphragm into the culture bottle, then cover the bottle cap on the culture bottle and screw it tightly, the light-transmitting diaphragm The ventilation tube passes through the bottle cap; (4) Adjust the color and light intensity of the LED light to start tissue culture, and turn on the micro fan to dissipate heat from the culture bottle.

Compared with the prior art, the present invention has the following beneficial effects: the integrated light-controllable tissue culture device of the present invention provides good light conditions for plants that need to grow under different light qualities or single light quality, and reduces the cost of plant tissue culture. Time cost, improve the utilization rate of light, thereby improving the efficiency of plant tissue culture, more environmentally friendly; at the same time solve the problem of heat dissipation of the bottle body and unsafe placement of the bottle body, provide a good environment for plant cell tissue culture, improve the survival rate, and shorten the plant life. Cell tissue culture cycle, reduce cost.

In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below through specific embodiments and related drawings.

Description of drawings

Fig. 1 is a front view of an embodiment of the present invention;

Fig. 2 is a perspective view of an embodiment of the present invention;

Fig. 3 is another perspective view of the embodiment of the present invention;

Fig. 4 is a three-dimensional view of the bottle cap structure of the embodiment of the present invention;

Fig. 5 is a bottom view of the bottle cap of the embodiment of the present invention;

Fig. 6 is a structural diagram of an aluminum substrate according to an embodiment of the present invention;

Explanation of symbols in the figure: 1-cultivation frame, 2-junction box, 3-bottle cap, 4-clamping device, 5-culture bottle, 6-translucent diaphragm, 7-power supply box, 8-air vent, 9-fan Switch, 10-power switch, 11-miniature fan, 12-air vent, 13-intensity control switch, 14-power cord, 15-aluminum substrate, 16-LED lamp, 17-ventilating tube, 18-beam, 19-containment tank, 20-bottle tank.

Detailed ways

As shown in Figures 1 to 6, an integrated light-controllable tissue culture device includes a culture frame 1, and a bottle groove 20 for placing a culture bottle 5 is arranged on the culture frame 1, and the side wall of the bottle body is perpendicular to the bottom of the bottle. It is beneficial to the absorption of light by cells and tissues. The culture bottle 5 is provided with a bottle cap 3, and the lower side of the bottle cap 3 is provided with LED lights 16 of different colors. The ratio of each color can be determined according to the actual situation. The light-transmitting diaphragm is used to isolate the bottle cap to prevent the bottle cap from being polluted after use. When cleaning, it only needs to clean the culture bottle body and the light-transmitting diaphragm; the culture rack is used to place and fix the culture bottle to avoid Stability or touch problems damage the culture bottle; the culture bottle 5 is provided with a light-transmitting diaphragm 6 for isolating the bottle cap 3 and the inner cavity of the culture bottle 5, and the light-transmitting diaphragm 6 is provided with a ventilating tube that passes through the bottle cap 3 upwards 17. The light-transmitting diaphragm 6 is made of optical quartz glass material, which has high light transmittance and heat resistance; the vent tube can avoid the direct contact between the mechanism in the bottle and the lamp cap and cause the bottle cap to be polluted; Or plants grown with single light quality can provide good light conditions, reduce the time cost of plant tissue culture, improve the utilization rate of light to improve the efficiency of plant tissue culture, and be more environmentally friendly.

In this embodiment, the bottom of the culture frame 1 is provided with a holding tank 19 directly below the bottle tank 20, and the bottom of the bottle tank 20 is provided with a ventilation hole 12 communicating with the holding tank, and a micro fan is arranged in the holding tank 19. 11.

In this embodiment, a clamping device 40 for clamping and fixing the culture bottle is provided on the side of the bottle tank 20. The clamping device adopts a spiral method, which has a simple structure and good effect; The side is used to control the fan switch 9 of the miniature fan 11; solve the problem of heat dissipation and unsafe placement of the culture bottle, prevent water droplets from being produced on the light-transmitting diaphragm, and affect the utilization rate of light.

In this embodiment, the bottle cap 3 is composed of an aluminum substrate 15 and a cover covered on the aluminum substrate 15, wherein the aluminum substrate adopts a composite board of a circuit board and a heat dissipation plate, which is beneficial to heat dissipation and reduces the risk of water droplets generated by the bottle cap. Probability; the bottle cap 3 is screwed to the culture bottle 5, the LED lamp 16 is arranged on the lower side of the aluminum substrate 15, and the aluminum substrate 15 and the cover are provided with perforations for the air pipe 17 to pass upwards .

In this embodiment, a crossbeam 18 is arranged on the top of the cultivation frame 1, and a power supply box 7 is arranged on the side of the crossbeam 18. The power supply box 7 includes power switches of three light colors to control different light colors in the lamp cover. The lamp beads work; the lower side of the crossbeam 18 is provided with a junction box 2 located above the bottle cap, and a power line 14 is connected between the junction box and the bottle cap. shrinking device to solve the problem of thread entanglement; the bottle cap is provided with an intensity control switch 13 for controlling the intensity of light to control and adjust the intensity of light of the LED lamp, which can be controlled according to the growth needs of cell tissues.

A method for cultivating with an integrated light-controllable tissue culture device as described above, comprising the following steps: (1) putting the treated plant tissue into a culture bottle; (2) putting the culture bottle into a bottle groove of a culture rack and use the clamping device to fix the culture bottle; (3) Put the light-transmitting diaphragm into the culture bottle, then put the cap on the culture bottle and screw it tightly, and the air tube on the light-transmitting diaphragm passes through the bottle cap. (4) Adjust the color and light intensity of the LED light to start tissue culture, and turn on the micro fan to dissipate heat from the culture bottle.

The above-listed preferred embodiments have further described the purpose, technical solutions and advantages of the present invention in detail. It should be understood that the above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Within the spirit and principles of the present invention, any modifications, equivalent replacements, improvements, etc., shall be included within the protection scope of the present invention.

Claims (6)

1. A kind of 1. tissue culture device of integral type controllable light, it is characterised in that：Including culturing rack, it is provided with to place on culturing rack The bottle groove of blake bottle, bottle cap is provided with the blake bottle, the bottle cap downside is provided with the LED of different colours, culture It is provided with to isolate bottle cap and the printing opacity barrier film of blake bottle inner chamber in bottle, is provided with the printing opacity barrier film and passes bottle cap upwards Permeability cell.
2. 2. the tissue culture device of integral type controllable light according to claim 1, it is characterised in that：The culturing rack bottom is set There is the holding tank immediately below bottle groove, bottle trench bottom is laid with the air-vent communicated with holding tank, set in the holding tank There is mini-fan.
3. 3. the tissue culture device of integral type controllable light according to claim 2, it is characterised in that：It is provided with positioned at bottle groove sidepiece To the clamping device for the blake bottle that is fixedly clamped, it is provided with culturing rack positioned at bottle groove side controlling the fan of mini-fan Switch.
4. 4. the tissue culture device of integral type controllable light according to claim 1, it is characterised in that：The bottle cap by aluminium base and The lid composition covered on aluminium base, the bottle cap are threadingly attached on blake bottle, and the LED is arranged under aluminium base Side, the perforation passed upwards for permeability cell is offered on the aluminium base and lid.
5. 5. the tissue culture device of integral type controllable light according to claim 1, it is characterised in that：It is horizontal positioned at the top of culturing rack Provided with crossbeam, the side of crossbeam is provided with power supply box, and the junction box being provided with the downside of the crossbeam above bottle cap is described to connect Power line is connected between line case and bottle cap, is provided with to control the intensity control switches of intensity of illumination on the bottle cap.
6. A kind of 6. cultural method of the tissue culture device of integral type controllable light as claimed in claim 3, it is characterised in that：Including following Step：（1）Plant tissue after processing is put into blake bottle；（2）Blake bottle is put into the bottle groove of culturing rack, and utilizes folder Tight device fixes blake bottle；（3）Printing opacity barrier film is packed into blake bottle, bottle cap is then covered on blake bottle and is screwed, thoroughly Permeability cell on light barrier film passes from bottle cap；（4）Adjust the color of LED and intensity of light starts tissue cultures, open micro- Type fan radiates to blake bottle.