CN105400694A - Cell culture system - Google Patents
Cell culture system Download PDFInfo
- Publication number
- CN105400694A CN105400694A CN201510961812.7A CN201510961812A CN105400694A CN 105400694 A CN105400694 A CN 105400694A CN 201510961812 A CN201510961812 A CN 201510961812A CN 105400694 A CN105400694 A CN 105400694A
- Authority
- CN
- China
- Prior art keywords
- casing
- bottle
- cell culture
- pipeline
- box body
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a cell culture system. The cell culture system comprises a the cell culture system comprises a box body, an air pump, and a culture flask; the lower end of the box body is provided with supporting legs; each of the left side wall and the right side wall of the box body is provided with an operation hole; each operation hole is provided with a rubber operating glove; the upper end of the box body is provided with a vacuumizing hole; the vacuumizing hole is connected with the air pump arranged outside the box body via a first pipeline; the culture flask is arranged on the bottom surface of the box body; the culture flask comprises a flask cover and a flask body; the culture flask is provided with an air hole; the air hole is connected with a second pipeline, wherein the upper end of the box body is penetrated by the second pipeline, and the second pipeline is stretched out from the box body; a rubber sealing ring is arranged between the second pipeline and the air hole; a magnetic stirring apparatus is arranged below the box body and the culture flask; and a magnetic stirring bar is delivered into the culture flask. The cell culture system is simple in structure, and is convenient to use; and the culture flask is placed at a vacuum environment, so that pollution rate of cells in the culture flask is reduced.
Description
Technical field
The invention belongs to cell culture apparatus field, relate in particular to a kind of cell culture apparatus.
Background technology
The success of cell cultures depends on that Cell protection is from microbiological contamination such as bacterium, fungi and viruses to a great extent.The work top of non-sterile article, substratum, the air particles with microorganism, sordid incubator and pollution is all the sources causing microbial contamination.Culturing cell, by after bacterial contamination, there will be nutrient solution and becomes muddiness, pH change.Also the nutrient solution visual inspection had, without how many changes, can only find that thalline just knows pollution under mirror.So it is very important that a raising cell exempts from contaminated cell culture apparatus concerning culturing cell.
Summary of the invention
For prior art, the object of this invention is to provide a kind of cell culture apparatus.
The object of the invention is to be achieved by following technical proposals:
A kind of cell culture apparatus, comprise: casing, off-gas pump and culturing bottle, described casing is cuboid, the lower end of casing is provided with feet, the left side wall of casing and right side wall are respectively equipped with handle hole, handle hole is provided with rubber operation gloves, be provided with one in the upper end of casing and vacuumize hole, this is vacuumized hole and is linked by the first pipeline and the off-gas pump being positioned at casing outside; Wherein, the bottom surface of casing is provided with a culturing bottle, described culturing bottle comprises bottle cap and bottle, the bottom cover of bottle has one first annulus, the middle part cover of bottle has one second annulus, the lower end of the first annulus and the bottom surface of casing are weldingly connected, the upper end of the first annulus is weldingly connected by a vertical union lever and the second annulus, culturing bottle is provided with a ventilating pit, this ventilating pit is connected with through casing upper end the second pipeline passed into outside casing, and is provided with rubber seal between the second pipeline and ventilating pit; Be provided with a magnetic stirring apparatus in the below of casing and culturing bottle, and in culturing bottle, be provided with rotation magneton.
In technique scheme, described feet is four.
In technique scheme, the top of described handle hole is provided with an operation port lid, this operation port lid and cabinet exterior hinged.
In technique scheme, be provided with a switch cover in the side of described casing, side and the casing of switch cover are hinged.
In technique scheme, described ventilating pit is arranged on above the bottle cap of culturing bottle.
Cell culture apparatus structure of the present invention is simple, easy to use, culturing bottle is placed in a vacuum environment, reduces the pollution probability of culturing bottle inner cell.
Accompanying drawing explanation
Fig. 1 is the structural scheme of mechanism of cell culture apparatus of the present invention.
Wherein, 1 is casing, and 2 is operation port lid, and 3 is rubber operation gloves, and 4 is the first annulus, 5 is magnetic stirring apparatus, and 6 is feet, and 7 is off-gas pump, and 8 is the first pipeline, and 9 is switch cover, 10 is rubber seal, and 11 is the second pipeline, and 12 is culturing bottle, and 13 is the second annulus, and 14 is union lever.
Embodiment
Below in conjunction with accompanying drawing, cell culture apparatus of the present invention is described in detail.
As shown in Figure 1, a kind of cell culture apparatus, comprise: casing 1, off-gas pump and tubular culturing bottle 13, casing is cuboid, the lower end of casing is provided with four feets 6, the left side wall of casing and right side wall are respectively equipped with handle hole, and handle hole is provided with rubber operation gloves 3, these operation gloves may be used for user to the culturing bottle in casing with regard to line operate.The top of handle hole is provided with an operation port lid, this operation port lid and cabinet exterior hinged, when user carries out operation with operator cover, opening operation port lid; After user has operated, shutoff operation port lid 2, this operation port lid can play the effect of a protection to operation gloves.Be provided with a switch cover 9 in the side of casing, side and the casing of switch cover are hinged, for putting into or taking out culturing bottle.
Be provided with one in the upper end of casing and vacuumize hole, this ventilating pit is connected with the off-gas pump being positioned at casing outside by the first pipeline 8; Wherein, the bottom surface of casing is provided with a culturing bottle, culturing bottle comprises bottle cap and bottle, the bottom cover of bottle has one first annulus, the middle part cover of bottle has one second annulus, the lower end of the first annulus 4 and the bottom surface of casing are weldingly connected, and the upper end of the first annulus is weldingly connected by a vertical union lever 14 and the second annulus.First annulus is used for not moving bottom fixing culturing bottle, and the second annulus is used for preventing culturing bottle from toppling over.
On the bottle cap of culturing bottle, be provided with a ventilating pit, this ventilating pit is connected with through casing upper end the second pipeline passed into outside casing, and is provided with rubber seal 10 between the second pipeline 11 and ventilating pit; Be provided with a magnetic stirring apparatus 5 in the below of casing and culturing bottle, and in culturing bottle, be provided with rotation magneton (not shown), for stirring the material in culturing bottle.
The using method of cell culture apparatus of the present invention is: open switch cover, is set on the bottle of culturing bottle by the first annulus and the second annulus, is inserted into above the bottle cap of culturing bottle, closing switch lid by the second pipeline 11.Vacuumize with off-gas pump 7 pairs of box houses.Open magnetic stirring apparatus, the rotation magneton in culturing bottle starts to rotate, and starts to carry out cell cultures.
Above to invention has been exemplary description; should be noted that; when not departing from core of the present invention, any simple distortion, amendment or other those skilled in the art can not spend the equivalent replacement of creative work all to fall into protection scope of the present invention.
Claims (5)
1. a cell culture apparatus, it is characterized in that, comprise: casing, off-gas pump and culturing bottle, described casing is cuboid, the lower end of casing is provided with feet, and the left side wall of casing and right side wall are respectively equipped with handle hole, handle hole is provided with rubber operation gloves, be provided with one in the upper end of casing and vacuumize hole, this is vacuumized hole and is linked by the first pipeline and the off-gas pump being positioned at casing outside; Wherein, the bottom surface of casing is provided with a culturing bottle, described culturing bottle comprises bottle cap and bottle, the bottom cover of bottle has one first annulus, the middle part cover of bottle has one second annulus, the lower end of the first annulus and the bottom surface of casing are weldingly connected, the upper end of the first annulus is weldingly connected by a vertical union lever and the second annulus, culturing bottle is provided with a ventilating pit, this ventilating pit is connected with through casing upper end the second pipeline passed into outside casing, and is provided with rubber seal between the second pipeline and ventilating pit; Be provided with a magnetic stirring apparatus in the below of casing and culturing bottle, and in culturing bottle, be provided with rotation magneton.
2. cell culture apparatus according to claim 1, is characterized in that, described feet is four.
3. cell culture apparatus according to claim 1, is characterized in that, the top of described handle hole is provided with an operation port lid, this operation port lid and cabinet exterior hinged.
4. cell culture apparatus according to claim 1, is characterized in that, is provided with a switch cover in the side of described casing, and side and the casing of switch cover are hinged.
5. cell culture apparatus according to claim 1, is characterized in that, described ventilating pit is arranged on above the bottle cap of culturing bottle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510961812.7A CN105400694A (en) | 2015-12-21 | 2015-12-21 | Cell culture system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510961812.7A CN105400694A (en) | 2015-12-21 | 2015-12-21 | Cell culture system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105400694A true CN105400694A (en) | 2016-03-16 |
Family
ID=55466459
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510961812.7A Pending CN105400694A (en) | 2015-12-21 | 2015-12-21 | Cell culture system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105400694A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111542592A (en) * | 2017-11-08 | 2020-08-14 | 株式会社Ihi | Connection unit for cell culture device, incubator device, and cell culture device |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202063921U (en) * | 2011-05-24 | 2011-12-07 | 珠海美华医疗科技有限公司 | Full-automatic blood culture instrument |
| CN202595126U (en) * | 2012-05-22 | 2012-12-12 | 中国水产科学研究院东海水产研究所 | Primary culture device of microalgae |
| CN102962851A (en) * | 2012-12-11 | 2013-03-13 | 哈尔滨商业大学 | Multi-purpose glove box |
| CN202849415U (en) * | 2012-11-03 | 2013-04-03 | 黑龙江八一农垦大学 | Double-layer biological culture flask |
| CN103589638A (en) * | 2013-11-12 | 2014-02-19 | 罗火生 | Pneumatic self-circulation animal cell culture bioreactor and application method thereof |
| CN203715618U (en) * | 2014-02-20 | 2014-07-16 | 陈樱 | Anaerobic bacteria incubator |
| CN204311060U (en) * | 2014-12-05 | 2015-05-06 | 安徽海峰分析测试科技有限公司 | Colony counter |
| CN204529856U (en) * | 2015-04-15 | 2015-08-05 | 付玉华 | A kind of anaerobic bacterium incubator |
| CN204550627U (en) * | 2015-03-10 | 2015-08-12 | 湖南师范大学 | A kind of air ventilation device for cell cultures |
| CN204676090U (en) * | 2015-05-12 | 2015-09-30 | 重庆市大足区珠溪中心卫生院 | A kind of inspection box for cell cultures |
| CN205223258U (en) * | 2015-12-21 | 2016-05-11 | 天津亿海生物科技有限公司 | Device for cell culture |
-
2015
- 2015-12-21 CN CN201510961812.7A patent/CN105400694A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202063921U (en) * | 2011-05-24 | 2011-12-07 | 珠海美华医疗科技有限公司 | Full-automatic blood culture instrument |
| CN202595126U (en) * | 2012-05-22 | 2012-12-12 | 中国水产科学研究院东海水产研究所 | Primary culture device of microalgae |
| CN202849415U (en) * | 2012-11-03 | 2013-04-03 | 黑龙江八一农垦大学 | Double-layer biological culture flask |
| CN102962851A (en) * | 2012-12-11 | 2013-03-13 | 哈尔滨商业大学 | Multi-purpose glove box |
| CN103589638A (en) * | 2013-11-12 | 2014-02-19 | 罗火生 | Pneumatic self-circulation animal cell culture bioreactor and application method thereof |
| CN203715618U (en) * | 2014-02-20 | 2014-07-16 | 陈樱 | Anaerobic bacteria incubator |
| CN204311060U (en) * | 2014-12-05 | 2015-05-06 | 安徽海峰分析测试科技有限公司 | Colony counter |
| CN204550627U (en) * | 2015-03-10 | 2015-08-12 | 湖南师范大学 | A kind of air ventilation device for cell cultures |
| CN204529856U (en) * | 2015-04-15 | 2015-08-05 | 付玉华 | A kind of anaerobic bacterium incubator |
| CN204676090U (en) * | 2015-05-12 | 2015-09-30 | 重庆市大足区珠溪中心卫生院 | A kind of inspection box for cell cultures |
| CN205223258U (en) * | 2015-12-21 | 2016-05-11 | 天津亿海生物科技有限公司 | Device for cell culture |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111542592A (en) * | 2017-11-08 | 2020-08-14 | 株式会社Ihi | Connection unit for cell culture device, incubator device, and cell culture device |
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|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160316 |