CN105420073A - Anaerobe culture bag and method for culturing anaerobe by using anaerobe culture bag - Google Patents
Anaerobe culture bag and method for culturing anaerobe by using anaerobe culture bag Download PDFInfo
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- CN105420073A CN105420073A CN201511001103.0A CN201511001103A CN105420073A CN 105420073 A CN105420073 A CN 105420073A CN 201511001103 A CN201511001103 A CN 201511001103A CN 105420073 A CN105420073 A CN 105420073A
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
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- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
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- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/30—Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
The invention discloses an anaerobe culture bag and a method for culturing anaerobe by using the anaerobe culture bag, which relate to the field of medical instruments. A main body of the culture bag is a bag body with one open side, a self-sealing tape is arranged at an opening of the main body of the culture bag, two bearing zones are arranged on the inner wall of the main body of the culture bag, a reagent capable of generating CO2 is placed on the bearing zone, an extraction opening is arranged at the bottom of the main body of the culture bag, and a detachable cover is arranged on the extraction opening. The method comprises the following steps: eliminating air in the aseptic anaerobe culture bag provided with flats; standing until oxygen concentration displayed on an oxygen indicator paper on the CO2 generation agent bag is zero, and placing the aseptic anaerobe culture bag provided with the flats in a culture environment required by anaerobe for culture. The anaerobe culture bag has the advantages of low cost, simple and easy operation, recycling and reuse, reduced cost, and good sealing performance, and can be used as a transport tool after a specimen is collected, and can be directly placed in a culture case for culture. The anaerobe culture bag is repeatedly usable, and can reduce cost of anaerobic cultivation.
Description
Technical field
The present invention relates to medical instruments field, particularly relate to a kind of anaerobic bacteria culture bag and utilize it to cultivate the method for anerobe.
Background technology
Although in recent years along with the improvement of sanitary condition, big area infected by microbes reduces year by year, but infected by microbes still occupies the major part of infectious diseases, in infected by microbes, anerobe (anaerobicbacteria) can cause the infection of human body different sites, comprise ecphyaditis, cholecystitis, otitis media, oral cavity infection, endocarditis, endometritis, cerebral abscess, myocardial necrosis, osteomyelitis, peritonitis, pyothorax, salpingitis, septic arthritis, liver abscess, sinusitis paranasal sinusitis, intestinal surgery or posttraumatic wound infection, pelvic inflammatory disease and microbemia etc., anerobe be a class under anaerobic than the bacterium grown in aerobic environment and can not the air of oxygen content more than 18% and/or gas concentration lwevel lower than 10% the epontic bacterium of solid medium.Anerobe lacks complete metabolic enzyme system, its energy metabolism is carried out in the mode of anaerobic fermentation, if anerobe exposes about 10 minutes in atmosphere, can affect anaerobic bacteria culture positive rate, therefore, it is preferred that the head of a bed samples, head of a bed plantation in the method for existing extraction anerobe.
When GLC for diagnosis of intra abdominal infections, need to cultivate anerobe, but at present anaerobic bacteria culture or need specific apparatus and operator's console, or need the anaerobic jar etc. of high price, trivial operations or cost higher or deposit inconvenience, these products of what is more important are not suitable for head of a bed sampling, head of a bed plantation bacterium, in recent years the plastic culture bag utilizing polythene bag to seal also has been there is for these problems, but great majority need to scald heat-sealing print, although some product does not need to scald heat-sealing print, but need steel sealing strip to seal, and this treatment unit and treatment process directly affect culture dish for anaerobe putting in incubator.
Summary of the invention
The object of the present invention is to provide a kind of anaerobic bacteria culture bag and utilize it to cultivate the method for anerobe, thus solve the foregoing problems existed in prior art.
To achieve these goals, anaerobic bacteria culture bag of the present invention, the main body of described culture bag is the bag of a side opening, and the opening of described culture bag main body arranges self-styled band, described culture bag main body inner wall arranges two supporting regions, described supporting region is placed and generates CO
2reagent, bleeding point is set in the bottom of described culture bag main body, described bleeding point arranges detachable cover.
Preferably, described supporting region is arranged near the bottom of described culture bag main body.
Preferably, described generation CO
2reagent be contained in aseptic ventilative material; Described ventilative material comprises aseptic non-woven fabrics.
Preferably, on first supporting region, CO is equipped with in setting
2generate the venting bags of agent, second supporting region arranges the venting bags that reductive agent is housed or all arranges on two supporting regions and CO is housed
2generate the venting bags of reductive agent.
More preferably, CO is being housed
2generate the venting bags of agent and CO is housed
2the venting bags of generation reductive agent arranges oxygen indicator bar.
Preferably, the quantity of described self-styled band is 2, and two self-styled bands be arranged in parallel.
Preferably, described bleeding point arranges unidirectional extraction valve or latex plug.
Preferably, the material of described culture bag main body is transparent material.
Use anaerobic bacteria culture bag to cultivate a method for anerobe, described method comprises:
The flat board and control group flat board that are coated with target anerobe are placed on aseptic anaerobic bacteria culture bag respectively;
The self-styled band of closed described anaerobic bacteria culture bag;
By bleeding point, the air in described anaerobic bacteria culture bag is got rid of;
Leave standstill until CO
2the oxygen indicator bar display oxygen concentration generated on agent bag is till zero, cultivates being equipped with in culture environment that dull and stereotyped anaerobic bacteria culture bag is placed on needed for anerobe.
Preferably, while flat board being placed into anaerobic bacteria culture bag, CO will be housed
2generate first supporting region that agent venting bags is placed into anaerobic bacteria culture bag, breathe freely be placed on second supporting region of anaerobic bacteria culture bag by being equipped with reductive agent, or arrange on two supporting regions CO is housed
2the venting bags of generation type reductive agent.
The invention has the beneficial effects as follows:
Anaerobic bacteria culture bag opening portion of the present invention uses dual self-styled band sealing, puts into carbonic acid gas generation compound and high-efficiency reducing agent with oxygen consumed in culture bag, can be easy in culture bag, form anaerobic condition, be conducive to the growth of anerobe; Of the present invention cheap, operation is simple; Culture bag is used to cultivate the method for anerobe simply, efficiently.
Anaerobic bacteria culture bag size of the present invention is less, is convenient for carrying, and cultivates at once after being beneficial to head of a bed sampling; Repeatedly reusable, save cost; Use dual self-styled band, improve sealing effectiveness; Low price, can reduce medical treatment cost; Do not need specific apparatus, easy to operate; Good seal performance, can be used as the transportation means after head of a bed collection of specimens, also directly can put into incubator and cultivate; Easily deposit, when putting into constant incubator, once just can cultivate multiple sample; Anaerobic bacteria culture bag is reusable, reduces the cost of Anaerobic culturel.
Accompanying drawing explanation
Fig. 1 is the schematic front view of described anaerobic bacteria culture bag;
Fig. 2 is equipped with dull and stereotyped schematic front view in Fig. 1;
1 represents culture bag main body, and 2 represent self-styled band, and 3 represent extraction valve, and 4 represent the first supporting region, and 5 represent the second supporting region, and 6 represent dull and stereotyped.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with accompanying drawing, the present invention is further elaborated.Should be appreciated that embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
See figures.1.and.2, anaerobic bacteria culture bag described in the present embodiment, the main body of described culture bag is the bag of a side opening, and the opening of described culture bag main body arranges self-styled band, described culture bag main body inner wall arranges two supporting regions, described supporting region is placed and generates CO
2reagent, bleeding point is set in the bottom of described culture bag main body, described bleeding point arranges detachable cover.More detailed explanation illustrates:
(1) described supporting region is arranged near the bottom of described culture bag main body.First supporting region is arranged CO is housed
2generate the venting bags of agent, second supporting region arranges the venting bags that reductive agent is housed or all arranges on two supporting regions and CO is housed
2generate the venting bags of reductive agent.CO is being housed
2generate the venting bags of agent and CO is housed
2the venting bags of generation reductive agent arranges oxygen indicator bar.
(2) described generation CO
2reagent be contained in ventilative material, described ventilative material comprises aseptic non-woven fabrics.
(3) quantity of described self-styled band is 2, and two self-styled bands be arranged in parallel.Described bleeding point arranges unidirectional extraction valve or latex plug.The material of described culture bag main body is transparent material.
Use anaerobic bacteria culture bag to cultivate a method for anerobe, described method comprises: the flat board and control group flat board that are coated with target anerobe are placed on aseptic anaerobic bacteria culture bag respectively; The self-styled band of closed described anaerobic bacteria culture bag; By bleeding point, the air in described anaerobic bacteria culture bag is got rid of; Leave standstill until CO
2the oxygen indicator bar display oxygen concentration generated on agent bag is till zero, cultivates being equipped with in culture environment that dull and stereotyped anaerobic bacteria culture bag is placed on needed for anerobe.
(1), while flat board being placed into anaerobic bacteria culture bag, CO will be housed
2generate first supporting region that agent venting bags is placed into anaerobic bacteria culture bag, breathe freely be placed on second supporting region of anaerobic bacteria culture bag by being equipped with reductive agent, or arrange on two supporting regions CO is housed
2the venting bags of generation type reductive agent.
Specific examples
Use sterilizing platinum loop scraping anerobe Clostridiumbotulinum (Clostridium botulinum) bacterium colony, molten to anerobe diluent (add the materials such as L-cystein:L-halfcystine and reduce redox potential) 9ml, carry out 10 times of dilutions again, be diluted to 104 times always, preparation stoste, 10 times of diluents, 100 times of diluents, 1000 times of diluents, 10000 times of diluents, be inoculated into by each diluent on the GAM agar plates of just preparation, often kind of concentration samples inoculates two culture dish;
Xitix is put into non-woven fabrics and make carbonic acid gas generation reductive agent bag;
Transparent airtight plastic bag put into by the culture dish inoculated, and puts into carbonic acid gas simultaneously and generates reductive agent bag, reductive agent bag surface mount oxygen show bar;
Two the self-styled band sealings in opening portion, after oxygen indicator bar display oxygen concentration near-zero, put into incubator 37 DEG C to cultivate, cultivate and observe colony number and bacterium colony size after 72 hours, first control group uses a self-styled band, all the other conditions are identical, and find that the bacterium colony size in two self-styled band sacks is more with colony number colony number compared with a self-styled band, bacterium colony size is larger; Second control group uses anaerobic jar to carry out cellar culture, finds that experimental group and control group colony number and bacterium colony size do not have significant difference.
By adopting technique scheme disclosed by the invention, obtain effect useful as follows: anaerobic bacteria culture bag opening portion of the present invention uses dual self-styled band sealing, in culture bag, put into carbonic acid gas generate compound and high-efficiency reducing agent with oxygen consumed, can be easy in culture bag, form anaerobic condition, be conducive to the growth of anerobe; Of the present invention cheap, operation is simple; Culture bag is used to cultivate the method for anerobe simply, efficiently.
Anaerobic bacteria culture bag size of the present invention is less, is convenient for carrying, and cultivates at once after being beneficial to head of a bed sampling; Repeatedly reusable, save cost; Use dual self-styled band, improve sealing effectiveness; Low price, can reduce medical treatment cost; Do not need specific apparatus, easy to operate; Good seal performance, can be used as the transportation means after head of a bed collection of specimens, also directly can put into incubator and cultivate; Easily deposit, when putting into constant incubator, once just can cultivate multiple sample; Anaerobic bacteria culture bag is reusable, reduces the cost of Anaerobic culturel.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should look protection scope of the present invention.
Claims (10)
1. an anaerobic bacteria culture bag, is characterized in that, the main body of described culture bag is the bag of a side opening, and the opening of described culture bag main body arranges self-styled band, and described culture bag main body inner wall arranges two supporting regions, described supporting region is placed and generates CO
2reagent, bleeding point is set in the bottom of described culture bag main body, described bleeding point arranges detachable cover.
2. anaerobic bacteria culture bag according to claim 1, it is characterized in that, described supporting region is arranged near the bottom of described culture bag main body.
3. anaerobic bacteria culture bag according to claim 1, is characterized in that, described generation CO
2reagent be contained in aseptic ventilative material; Described ventilative material comprises aseptic non-woven fabrics.
4. anaerobic bacteria culture bag according to claim 1, is characterized in that, first supporting region is arranged CO is housed
2generate the venting bags of agent, second supporting region arranges the venting bags that reductive agent is housed or all arranges on two supporting regions and CO is housed
2generate the venting bags of reductive agent.
5. anaerobic bacteria culture bag according to claim 4, is characterized in that, CO is being housed
2generate the venting bags of agent and CO is housed
2the venting bags of generation reductive agent arranges oxygen indicator bar.
6. anaerobic bacteria culture bag according to claim 1, it is characterized in that, the quantity of described self-styled band is 2, and two self-styled bands be arranged in parallel.
7. anaerobic bacteria culture bag according to claim 1, is characterized in that, described bleeding point arranges unidirectional extraction valve or latex plug.
8. anaerobic bacteria culture bag according to claim 1, it is characterized in that, the material of described culture bag main body is transparent material.
9. use anaerobic bacteria culture bag described in claim 1 to cultivate a method for anerobe, it is characterized in that, described method comprises:
The flat board and control group flat board that are coated with target anerobe are placed on aseptic anaerobic bacteria culture bag respectively;
The self-styled band of closed described anaerobic bacteria culture bag;
By bleeding point, the air in described anaerobic bacteria culture bag is got rid of;
Leave standstill until CO
2the oxygen indicator bar display oxygen concentration generated on agent bag is till zero, cultivates being equipped with in culture environment that dull and stereotyped anaerobic bacteria culture bag is placed on needed for anerobe.
10. method according to claim 9, is characterized in that, while flat board being placed into anaerobic bacteria culture bag, CO will be housed
2generate first supporting region that agent venting bags is placed into anaerobic bacteria culture bag, breathe freely be placed on second supporting region of anaerobic bacteria culture bag by being equipped with reductive agent, or arrange on two supporting regions CO is housed
2the venting bags of generation type reductive agent.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201511001103.0A CN105420073A (en) | 2015-12-28 | 2015-12-28 | Anaerobe culture bag and method for culturing anaerobe by using anaerobe culture bag |
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| CN201511001103.0A CN105420073A (en) | 2015-12-28 | 2015-12-28 | Anaerobe culture bag and method for culturing anaerobe by using anaerobe culture bag |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967591A (en) * | 2017-06-01 | 2017-07-21 | 田金静 | A kind of clinical microbiology anaerobe samples storage bottle |
| CN109022256A (en) * | 2018-07-17 | 2018-12-18 | 广东药科大学附属第医院 | Anaerobic culturel bag with Anaerobic indicator |
| WO2021160790A1 (en) | 2020-02-12 | 2021-08-19 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Sampling kit for the transport of sputum |
| GB2627864A (en) * | 2023-01-20 | 2024-09-04 | Abbvie Inc | Clostridium botulinum serotype A neurotoxin (BoNT/A) sequence variants |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2083496A (en) * | 1980-08-20 | 1982-03-24 | Mitsubishi Gas Chemical Co | Cultivating anaerobic bacterium |
| CN2505826Y (en) * | 2001-09-25 | 2002-08-14 | 邹渝 | Prereduced anaerobic bacteria specimen sampling, delivery apparatus |
| CN2505485Y (en) * | 2001-09-14 | 2002-08-14 | 邹渝 | Pre-reduced anaerobic medium bag |
| CN204198730U (en) * | 2014-11-14 | 2015-03-11 | 重庆庞通医疗器械有限公司 | Anerobe, microaerobe and need carbonic acid gas bacterium culture bag |
| CN205275577U (en) * | 2015-12-28 | 2016-06-01 | 北京澳利文生物科技有限公司 | Anaerobe culture bag |
-
2015
- 2015-12-28 CN CN201511001103.0A patent/CN105420073A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2083496A (en) * | 1980-08-20 | 1982-03-24 | Mitsubishi Gas Chemical Co | Cultivating anaerobic bacterium |
| CN2505485Y (en) * | 2001-09-14 | 2002-08-14 | 邹渝 | Pre-reduced anaerobic medium bag |
| CN2505826Y (en) * | 2001-09-25 | 2002-08-14 | 邹渝 | Prereduced anaerobic bacteria specimen sampling, delivery apparatus |
| CN204198730U (en) * | 2014-11-14 | 2015-03-11 | 重庆庞通医疗器械有限公司 | Anerobe, microaerobe and need carbonic acid gas bacterium culture bag |
| CN205275577U (en) * | 2015-12-28 | 2016-06-01 | 北京澳利文生物科技有限公司 | Anaerobe culture bag |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967591A (en) * | 2017-06-01 | 2017-07-21 | 田金静 | A kind of clinical microbiology anaerobe samples storage bottle |
| CN106967591B (en) * | 2017-06-01 | 2019-07-09 | 田金静 | A kind of clinical microbiology anaerobe samples storage bottle |
| CN109022256A (en) * | 2018-07-17 | 2018-12-18 | 广东药科大学附属第医院 | Anaerobic culturel bag with Anaerobic indicator |
| CN109022256B (en) * | 2018-07-17 | 2022-05-13 | 广东药科大学附属第一医院 | Anaerobic culture bag with anaerobic indicator |
| WO2021160790A1 (en) | 2020-02-12 | 2021-08-19 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Sampling kit for the transport of sputum |
| GB2627864A (en) * | 2023-01-20 | 2024-09-04 | Abbvie Inc | Clostridium botulinum serotype A neurotoxin (BoNT/A) sequence variants |
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Application publication date: 20160323 |