CN109022256B - Anaerobic culture bag with anaerobic indicator - Google Patents
Anaerobic culture bag with anaerobic indicator Download PDFInfo
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- CN109022256B CN109022256B CN201810785388.9A CN201810785388A CN109022256B CN 109022256 B CN109022256 B CN 109022256B CN 201810785388 A CN201810785388 A CN 201810785388A CN 109022256 B CN109022256 B CN 109022256B
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- piston
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- air pressure
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- bag
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/14—Bags
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/20—Degassing; Venting; Bubble traps
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/40—Means for regulation, monitoring, measurement or control, e.g. flow regulation of pressure
Abstract
The invention relates to an anaerobic culture bag with an anaerobic indicator, which comprises a bag body and further comprises the anaerobic indicator, wherein the anaerobic indicator comprises a shell, a first piston and an opening device, and the shell is provided with a piston cavity; the bag body is provided with a one-way valve, and when the air pressure in the bag body is greater than the air pressure in the piston cavity, the air in the bag body flows into the piston cavity through the one-way valve; the opening device is arranged on the shell and used for being connected with a bottle mouth of an ampoule for containing an anaerobic indicator, and when the pressure in the piston cavity is greater than a preset value, the opening device is triggered to open the bottle mouth, so that gas in the piston cavity enters the ampoule. According to the anaerobic culture bag with the anaerobic indicator, the gas in the piston cavity enters the ampule bottle to realize the function of testing the gas in the anaerobic culture bag, and the anaerobic testing efficiency of the anaerobic culture bag is effectively improved.
Description
Technical Field
The invention belongs to the field of containers, and particularly relates to an anaerobic culture bag with an anaerobic indicator.
Background
The culture of anaerobic bacteria requires that the culture gas environment is anaerobic, the culture medium does not form peroxide harmful to the anaerobic bacteria, and the anaerobic environment and proper humidity should be maintained in the culture medium during the preparation and storage processes. At present, the prepared anaerobic culture medium is packaged and stored by adopting a breathable and water-permeable material. When the air humidity is too high, water permeates, the cultured gene humidity is too high, and the mould grows to lose efficacy; when the air humidity is too small, the water is lost, and the cultured genes are too dry and also lose efficacy; furthermore, the oxygen is permeated for a long time, and the culture medium is useless because peroxide harmful to anaerobic bacteria is formed by the oxygen culture.
Anaerobic indicators are used primarily in detecting spaces or containers that require anaerobic conditions, such as anaerobic culture bags in a microbiological chamber. The anaerobic bacteria indicator commonly used at present is usually prepared immediately (particularly glucose aqueous solution), the three are mixed in a test tube according to the proportion of 2:0.1:0.2 (milliliter), the test tube is boiled to be colorless, the test tube is quickly put into an anaerobic tank (bag), if the tank (bag) is anaerobic, the mixed liquid is still colorless, and if oxygen is remained, the mixed liquid is blue. The indicator is prepared into an anaerobic sterile state and sealed in an ampule. When the anaerobic state needs to be detected, the ampule bottle is put into an anaerobic jar (bag), and when the ampule bottle is used, the bottleneck is broken off, so that liquid in the ampule bottle is contacted with the anaerobic bag or gas in the anaerobic bottle. If the bottle body in the anaerobic jar (bag) is colorless, the bottleneck of the inlet air is blue, which indicates that the indicator is effective and the anaerobic jar (bag) is in an anaerobic state.
Disclosure of Invention
The invention aims to provide an anaerobic culture bag with an anaerobic indicator, which can conveniently detect gas in the bag by using the anaerobic indicator and can not pollute the gas in the bag.
For this purpose, the invention provides an anaerobic culture bag with an anaerobic indicator, which comprises a bag body and also comprises an anaerobic indicator, wherein the anaerobic indicator comprises a shell, a first piston and an opening device,
the housing is provided with a piston cavity, and the first piston is positioned in the piston cavity;
the bag body is provided with a one-way valve, and when the air pressure in the bag body is greater than the air pressure in the piston cavity, the air in the bag body flows into the piston cavity through the one-way valve;
the opening device is arranged on the shell and used for being connected with a bottle mouth of an ampoule for containing an anaerobic indicator, and when the pressure in the piston cavity is greater than a preset value, the opening device is triggered to open the bottle mouth, so that gas in the piston cavity enters the ampoule.
Preferably, the ampoule bottle further comprises a handle, wherein a containing cavity is formed in the handle and used for containing the ampoule bottle.
Preferably, the opening means is provided in the first piston.
Preferably, the opening device comprises an air pressure sensor, a driver and a cutting knife, the air pressure sensor is connected with the driver, the cutting knife is connected with the driver, and when the pressure in the piston cavity is greater than the preset value, the driver is triggered by the air pressure sensor to drive the cutting knife to move so as to cut the bottle mouth, so that the air in the piston cavity enters the Ann-cut bottle.
Preferably, the opening device comprises a mounting hole arranged on the first piston, one end of the mounting hole is arranged on the clamping nozzle, and the other end of the mounting hole is provided with the air pressure block; and a puncture needle extending to the clamping nozzle is arranged on the side surface of the air pressure block facing the clamping nozzle.
Preferably, a boss is arranged in the mounting hole, and the air pressure block abuts against the boss through an elastic body.
Preferably, the piston cavity is a cavity with two through ends, and the piston further comprises a second piston, and the second piston is located in the piston cavity.
Preferably, the lateral surface of casing is equipped with a depressed area, the bent axle is acceptd in the depressed area of telling, the bent axle includes telescopic link and handle, the one end of telescopic link with the connecting rod is connected, and the other end with the handle is connected, the lateral wall of depressed area has one with the opening that the handle corresponds is used for acceping the handle.
Preferably, the side wall of the piston cavity is provided with an exhaust valve, and when the first piston is close to the second piston, the gas in the piston cavity is exhausted outwards through the exhaust valve.
Compared with the prior art, the anaerobic culture bag with the anaerobic indicator provided by the invention has the advantages that the first piston is moved to enable the one-way valve of the bag body to be positioned on the side wall of the piston cavity, so that the gas in the bag partially flows into the piston cavity under the action of low pressure, then the first piston is moved again to pressurize the gas in the piston, so that the opening device can be started to open the bottle mouth of the An-cut bottle containing the anaerobic indicator, the gas in the piston cavity enters the An-cut bottle to realize the function of testing the gas in the anaerobic culture bag with the anaerobic indicator, and the anaerobic testing efficiency of the anaerobic culture bag is effectively improved.
Drawings
FIG. 1 is a schematic diagram of the construction of an embodiment of an anaerobic culture bag with an anaerobic indicator according to the present invention;
FIG. 2 is a schematic view showing the construction of a first piston and an opening means of the anaerobic culture bag of FIG. 1;
FIG. 3 is a schematic diagram of an embodiment of an anaerobic bag with an anaerobic indicator according to the present invention, wherein the first piston is in a starting position;
FIG. 4 is a schematic diagram of the structure of an embodiment of the anaerobic culture bag with an anaerobic indicator according to the present invention, wherein the puncture needle is in a state of puncturing the mouth of the ampoule;
in the figure:
10: a bag body; 11: a one-way valve; 20: a housing; 21: a piston cavity; 30: a first piston; 31: a handle; 40: an opening device; 41: gas pressure blocks; 42: an elastomer; 43: a needle; 44: a boss; 45: a clamping nozzle; 46: mounting holes; 50: cutting off the bottle; 51: a bottle mouth; 60: a second piston; 61: a rack; 62: a connecting rod; 63: a crankshaft; 64: a handle; 65: a gear.
Detailed Description
The invention will be further explained with reference to the drawings.
FIG. 1 is a schematic structural view of an embodiment of an anaerobic culture bag with an anaerobic indicator according to the present invention. Referring to fig. 1, the anaerobic culture bag includes a bag body 10 and an anaerobic indicator for detecting an anaerobic state of gas in the bag body 10.
The anaerobic indicator comprises a housing 20, a first piston 30 and an opening means 40, preferably arranged in the first piston 30. The housing 20 has a piston chamber 21, and the first piston 30 is located in the piston chamber 21. The opening device 40 is arranged on the shell 20 and is used for being connected with a bottle mouth 51 of an ampoule 50 for containing an anaerobic indicator, and when the pressure in the piston cavity 21 is greater than a preset value, the opening device 40 is triggered to open the bottle mouth 51, so that the gas in the piston cavity 21 enters the ampoule 50. The bag body 10 is provided with a one-way valve 11, and when the air pressure in the bag body 10 is greater than the air pressure in the piston cavity 21, the air in the bag body 10 flows into the piston cavity 21 through the one-way valve 11.
FIG. 2 is a schematic view of the structure of the first piston 30 and the opening device 40 of the anaerobic culture bag with an anaerobic indicator of FIG. 1. In the present embodiment, as shown in fig. 2, the opening device 40 includes a mounting hole 46 provided in the first piston 30, one end of the mounting hole 46 is provided in the locking nozzle 45, and the other end is provided with the air pressure block 41; the side of the air pressure block 41 facing the bayonet 45 is provided with a spike 43 extending to the bayonet 45. In other embodiments, the opening device 40 includes an air pressure sensor, an actuator, and a cutter, the air pressure sensor is connected to the actuator, the cutter is connected to the actuator, and when the pressure in the piston cavity 21 is greater than the preset value, the actuator is triggered by the air pressure sensor to drive the cutter to move to cut the bottle mouth 51, so that the air in the piston cavity 21 enters the ampoule 50.
The anaerobic culture bag further comprises a handle 31 connected with the first piston 30, and a containing cavity is arranged in the handle 31 and used for containing the ampule 50. A boss 44 is arranged in the mounting hole 46, and the air pressure block 41 abuts against the boss 44 through an elastic body 42.
In addition, the piston cavity 21 is a cavity with two through ends, and further includes a second piston 60, and the second piston 60 is located in the piston cavity 21. An exhaust valve is arranged on the side wall of the piston cavity 21, and when the first piston 30 is close to the second piston 60, the gas in the piston cavity 21 is exhausted outwards through the exhaust valve. The anaerobic culture bag further comprises a positioning mechanism, the positioning mechanism comprises a rack 61, a gear 65 meshed with the rack 61, a connecting rod 62 and a crankshaft 63, the rack 61 is connected to the second piston 60 along the axial direction of the piston cavity 21, the connecting rod 62 and the gear 65 are connected to the side face, back to the first piston 30, close to the second piston 60 and penetrate through the shell 20, and the crankshaft 63 is connected with the connecting rod 62. The outer side surface of the shell 20 is provided with a depressed area, the crankshaft 63 is accommodated in the depressed area, the crankshaft 63 comprises an expansion link and a handle 64, one end of the expansion link is connected with the connecting rod 62, the other end of the expansion link is connected with the handle 64, and the side wall of the depressed area is provided with a notch corresponding to the handle 64 and used for accommodating the handle 64. The position adjustment mechanism can adjust the position of the second piston 60, thereby adjusting the positions of the first piston 30 and the second piston 60 in the compressed gas and the test gas, so that the first piston 30 covers the position of the check valve 11 in the initial position.
The anaerobic test process of the anaerobic culture bag provided by the embodiment is explained below with reference to fig. 3 and 4.
FIG. 3 is a schematic structural view of an embodiment of an anaerobic culture bag according to the present invention, in which a first piston 30 is in a starting position. As shown in fig. 3, the exhaust valve of the piston chamber 21 is opened, the first piston 30 is pushed to approach the second piston 60 until the first piston 30 contacts the second piston 60 to exhaust the gas in the piston chamber 21 through the exhaust valve, and then the exhaust valve is closed.
Then, the first piston 30 is pulled reversely, so that the space in the piston cavity 21 is expanded, and the gas in the bag body 10 leaks into the piston cavity 21 from the check valve 11 under the action of the negative pressure in the piston cavity 21 until the gas pressure in the piston cavity 21 is the same as the gas pressure in the bag body 10.
Then, the first piston 30 is pushed close to the second piston 60 to compress the gas in the piston chamber 21, and since the check valve 11 of the pouch body 10 only allows the gas to flow from the pouch body 10 into the piston chamber 21, the gas in the piston chamber 21 does not return to the pouch body 10 when the first piston 30 compresses the gas in the piston chamber 21.
FIG. 4 is a schematic diagram showing the structure of an embodiment of an anaerobic culture bag according to the present invention, wherein a puncture needle 43 is in a state of puncturing a mouth 51 of an ampoule 50. As shown in FIG. 4, as the air pressure in the piston chamber 21 increases, the air pressure pushes the air pressure block 41 closer to the mouth 51 of the ampoule 50 until the spike 43 pierces the mouth 51 of the ampoule 50. The gas in the piston cavity 21 can slowly leak to the side of the gas pressure block 41 close to the mouth 51 of the ampoule 50, so that the gas can enter the ampoule 50, and the anaerobic gas is tested.
The anaerobic culture bag that this embodiment provided makes the check valve 11 of the bag body 10 be located the lateral wall of piston chamber 21 at removal first piston 30 to gaseous partial inflow piston chamber 21 under the low pressure in the bag, then remove first piston 30 again so that can set out opening device 40 and open the bottleneck 51 of the ann's dissection bottle 50 of splendid attire anaerobic indicator with the gaseous pressurization in the piston, make the gaseous entering in the piston chamber 21 realize carrying out the function tested to the gas in the anaerobic culture bag in the ann's dissection bottle 50, effectively improved anaerobic test efficiency of anaerobic culture bag.
It is to be understood that the present invention is not limited to the above-described embodiments, and that various changes and modifications may be made without departing from the spirit and scope of the invention, and it is intended to cover such changes and modifications as fall within the scope of the appended claims and equivalents thereof.
Claims (5)
1. An anaerobic culture bag with an anaerobic indicator comprises a bag body and is characterized by also comprising an anaerobic indicator, wherein the anaerobic indicator comprises a shell, a first piston and an opening device,
the housing is provided with a piston cavity, and the first piston is positioned in the piston cavity;
the bag body is provided with a one-way valve, and when the air pressure in the bag body is greater than the air pressure in the piston cavity, the air in the bag body flows into the piston cavity through the one-way valve;
the opening device is arranged on the shell and is used for being connected with a bottle mouth of an ampoule for containing an anaerobic indicator, and when the pressure in the piston cavity is greater than a preset value, the opening device is triggered to open the bottle mouth, so that gas in the piston cavity enters the ampoule;
the ampoule bottle is characterized by further comprising a handle, wherein a containing cavity is formed in the handle and used for containing the ampoule bottle;
the opening device is arranged in the first piston;
the opening device comprises an air pressure sensor, a driver and a cutting knife, the air pressure sensor is connected with the driver, the cutting knife is connected with the driver, and when the pressure in the piston cavity is greater than the preset value, the driver is triggered by the air pressure sensor to drive the cutting knife to move so as to cut the bottle mouth, so that the air in the piston cavity enters the An-cut bottle;
or the opening device comprises a mounting hole arranged on the first piston, one end of the mounting hole is provided with a clamping nozzle, and the other end of the mounting hole is provided with an air pressure block; and a puncture needle extending to the clamping nozzle is arranged on the side surface of the air pressure block facing the clamping nozzle.
2. The anaerobic culture bag with an anaerobic indicator according to claim 1, wherein a boss is provided in the mounting hole, and the air pressure block abuts against the boss through an elastic body.
3. The anaerobic culture bag with the anaerobic indicator according to claim 2, wherein the piston chamber is a chamber with two ends through, and further comprises a second piston, and the second piston is positioned in the piston chamber.
4. An anaerobic culture bag with an anaerobic indicator according to claim 3, wherein the outer side of the housing is provided with a recessed area, the crankshaft is accommodated in the recessed area, the crankshaft comprises a telescopic rod and a handle, one end of the telescopic rod is connected with the connecting rod, the other end of the telescopic rod is connected with the handle, and the side wall of the recessed area is provided with a gap corresponding to the handle for accommodating the handle.
5. The anaerobic culture bag with an anaerobic indicator according to claim 4, wherein the side wall of the piston chamber is provided with a vent valve through which the gas in the piston chamber is discharged to the outside when the first piston approaches the second piston.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810785388.9A CN109022256B (en) | 2018-07-17 | 2018-07-17 | Anaerobic culture bag with anaerobic indicator |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810785388.9A CN109022256B (en) | 2018-07-17 | 2018-07-17 | Anaerobic culture bag with anaerobic indicator |
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| CN109022256A CN109022256A (en) | 2018-12-18 |
| CN109022256B true CN109022256B (en) | 2022-05-13 |
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