CN105385582A - Novel anaerobic-bacterium culturing dish - Google Patents

Novel anaerobic-bacterium culturing dish Download PDF

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CN105385582A
CN105385582A CN201510966817.9A CN201510966817A CN105385582A CN 105385582 A CN105385582 A CN 105385582A CN 201510966817 A CN201510966817 A CN 201510966817A CN 105385582 A CN105385582 A CN 105385582A
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culture dish
lid
novel anaerobic
gate valve
inlet pipe
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韩作振
孙彬
闫华晓
赵辉
韩梅
赵延洋
孟瑞瑞
庄定祥
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Shandong University of Science and Technology
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/10—Petri dish
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals

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Abstract

本发明公开了一种新型厌氧菌培养皿，包括培养皿本体及盖体，所述培养皿本体与其上方的盖体通过螺纹连接，形成密闭的空腔。所述盖体顶部的一侧设置有进气管，进气管上配置有气体过滤器，进气管上还配置有第一闸阀，所述第一闸阀位于气体过滤器和盖体之间。所述盖体顶部的另一侧设置有出气管，出气管上具有用于放置氧气指示剂的球形观测腔，所述球形观测腔与盖体之间的出气管上设置有第二闸阀。本发明结构简单，实用性强，制造成本低，易于操作，便于广泛推广使用。

The invention discloses a novel anaerobic culture dish, which comprises a culture dish body and a cover body. The culture dish body and the upper cover body are connected by threads to form a closed cavity. An air inlet pipe is arranged on one side of the top of the cover, and a gas filter is arranged on the air inlet pipe, and a first gate valve is arranged on the air inlet pipe, and the first gate valve is located between the air filter and the cover body. The other side of the top of the cover is provided with an air outlet pipe, on which there is a spherical observation chamber for placing an oxygen indicator, and a second gate valve is arranged on the air outlet pipe between the spherical observation chamber and the cover. The invention has the advantages of simple structure, strong practicability, low manufacturing cost, easy operation and convenient popularization and use.

Description

一种新型厌氧菌培养皿A new type of anaerobic culture dish

技术领域technical field

本发明涉及地质微生物实验操作领域，具体涉及一种新型厌氧菌培养皿。The invention relates to the field of geological microorganism experiment operation, in particular to a novel anaerobic culture dish.

背景技术Background technique

培养皿，一般用于动植物细胞或微生物细胞的培养，往往由玻璃或塑料制成，结构简单。关于培养皿的技术性改进较多，除了常规的可以灭菌的具备不同直径的玻璃培养皿之外，还有一次性的培养皿。但是，这些培养皿大都是利用有氧环境进行细胞培养的。目前专门用于培养厌氧微生物的培养皿却较少。Petri dishes, generally used for the cultivation of animal and plant cells or microbial cells, are often made of glass or plastic and have a simple structure. There are many technical improvements on petri dishes. In addition to conventional glass petri dishes with different diameters that can be sterilized, there are also disposable petri dishes. However, most of these culture dishes utilize an aerobic environment for cell culture. At present, there are fewer petri dishes specially used for cultivating anaerobic microorganisms.

要培养厌氧菌，必须创造一个无氧的环境，有些厌氧菌甚至需要严格的无氧环境。通常用培养基中加入还原剂，或用物理、化学方法去除环境中的游离氧，以降低氧化还原电势。常用的厌氧菌培养方法有许多，例如夹层培养法、厌氧工作箱培养法、厌氧袋培养法、生物耗氧等方法。上述培养方法操作复杂，有些方法厌氧效果不理想。为了更便捷地培养厌氧微生物，有必要制作一种专门用于培养厌氧菌的培养皿，要求这种培养皿具有良好的密闭效果，能创造一种厌氧环境，同时培养过程简单易于操作。To cultivate anaerobic bacteria, an anaerobic environment must be created, and some anaerobic bacteria even require a strict anaerobic environment. Usually, reducing agents are added to the medium, or free oxygen in the environment is removed by physical and chemical methods to reduce the redox potential. There are many commonly used anaerobic culture methods, such as sandwich culture method, anaerobic work box culture method, anaerobic bag culture method, biological oxygen consumption and other methods. The above culture methods are complicated to operate, and some methods have unsatisfactory anaerobic effects. In order to cultivate anaerobic microorganisms more conveniently, it is necessary to make a petri dish specially used for cultivating anaerobic bacteria. This petri dish is required to have a good airtight effect, can create an anaerobic environment, and the cultivation process is simple and easy to operate .

发明内容Contents of the invention

针对上述现有技术的不足，本发明提供一种新型厌氧菌培养皿，该培养皿具备密封效果好，能提供厌氧环境，操作简单等特点。Aiming at the deficiencies of the above-mentioned prior art, the present invention provides a novel anaerobic culture dish, which has the characteristics of good sealing effect, ability to provide an anaerobic environment, and simple operation.

为了解决上述技术问题，本发明所采用的技术方案是：In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:

一种新型厌氧菌培养皿，包括培养皿本体及盖体，所述培养皿本体与其上方的盖体通过螺纹连接，形成密闭的空腔。所述盖体顶部的一侧设置有进气管，进气管上配置有气体过滤器，进气管上还配置有第一闸阀，所述第一闸阀位于气体过滤器和盖体之间。所述盖体顶部的另一侧设置有出气管，出气管上具有用于放置氧气指示剂的球形观测腔，所述球形观测腔与盖体之间的出气管上设置有第二闸阀。A novel anaerobic culture dish comprises a culture dish body and a cover body, and the culture dish body and the upper cover body are connected by threads to form a closed cavity. An air inlet pipe is arranged on one side of the top of the cover, and a gas filter is arranged on the air inlet pipe, and a first gate valve is also arranged on the air inlet pipe, and the first gate valve is located between the air filter and the cover body. An air outlet pipe is provided on the other side of the top of the cover, and the air outlet pipe has a spherical observation chamber for placing an oxygen indicator, and a second gate valve is arranged on the air outlet pipe between the spherical observation chamber and the cover.

优选地，所述进气管的一端与培养皿本体的内部相通，另一端可连接氮气气瓶。所述出气管的一端也与培养皿本体的内部相通，另一端与外界相通。Preferably, one end of the inlet pipe communicates with the inside of the culture vessel body, and the other end can be connected to a nitrogen cylinder. One end of the air outlet pipe also communicates with the inside of the culture dish body, and the other end communicates with the outside world.

优选地，所述气体过滤器为具有滤膜过滤层和活性炭的双层过滤器。Preferably, the gas filter is a double-layer filter with a membrane filter layer and activated carbon.

优选地，所述培养皿本体开口端的外侧壁上设置有外螺纹，盖体开口端的内侧壁上设置有内螺纹，外螺纹与内螺纹相适配。Preferably, the outer side wall of the opening end of the culture vessel body is provided with external threads, and the inner wall of the opening end of the lid is provided with internal threads, and the external threads are matched with the internal threads.

优选地，所述盖体的内侧壁上设置有环形的安装座，安装座靠近内螺纹的一侧配置有橡胶密封圈。Preferably, an annular mounting seat is provided on the inner side wall of the cover body, and a rubber sealing ring is arranged on a side of the mounting seat close to the internal thread.

优选地，所述培养皿本体和盖体均由透明玻璃制成，出气管也由透明玻璃制成。Preferably, both the culture vessel body and the cover are made of transparent glass, and the air outlet pipe is also made of transparent glass.

优选地，所述出气管由竖向管体和横向管体连接而成，横向管体与盖体的顶部平行。所述球形观测腔位于横向管体内，球形观测腔的内径大于横向管体的内径。Preferably, the air outlet pipe is formed by connecting a vertical pipe body and a horizontal pipe body, and the horizontal pipe body is parallel to the top of the cover. The spherical observation chamber is located in the transverse pipe body, and the inner diameter of the spherical observation chamber is larger than the inner diameter of the transverse pipe body.

通过采用上述技术方案，本发明的有益技术效果是：由于外螺纹与内螺纹相适配，以及在盖体内设置密封圈，可以保证培养皿密闭效果，经进气管充入氮气，排气管排出培养皿内原有的气体，使培养皿内的氧气含量下降，通过球形观测腔内的氧气指示剂的颜色变化，能够说明培养皿内的氧气含量是否达到了厌氧菌生长的要求。本发明结构简单，实用性强，制造成本低，易于操作，便于广泛推广使用。By adopting the above technical scheme, the beneficial technical effect of the present invention is: due to the matching of the external thread and the internal thread, and the sealing ring is set in the cover body, the airtight effect of the culture dish can be guaranteed, nitrogen is filled through the intake pipe, and the exhaust pipe is discharged. The original gas in the petri dish reduces the oxygen content in the petri dish, and the color change of the oxygen indicator in the spherical observation chamber can show whether the oxygen content in the petri dish meets the requirements for the growth of anaerobic bacteria. The invention has the advantages of simple structure, strong practicability, low manufacturing cost, easy operation and convenient popularization and use.

附图说明Description of drawings

图1是本发明一种新型厌氧菌培养皿的结构原理示意图。Fig. 1 is a schematic diagram of the structure and principle of a novel anaerobic culture dish of the present invention.

具体实施方式detailed description

下面结合附图对本发明进行详细说明：The present invention is described in detail below in conjunction with accompanying drawing:

结合图1，一种新型厌氧菌培养皿，包括培养皿本体1及盖体2，所述培养皿本体1与其上方的盖体2通过螺纹连接，形成密闭的空腔，所述培养皿本体1和盖体2均由透明玻璃制成。所述培养皿本体1开口端的外侧壁上设置有外螺纹3，盖体2开口端的内侧壁上设置有内螺纹4，所述外螺纹3与内螺纹4相适配。所述盖体1的内侧壁上设置有环形的安装座5，安装座5靠近内螺纹4的一侧配置有橡胶密封圈6。所述盖体2顶部的一侧设置有进气管7，进气管7的一端与培养皿本体1的内部相通，另一端可连接氮气气瓶。进气管7上配置有气体过滤器8，所述气体过滤器8优选具有滤膜过滤层和活性炭的双层过滤器。In conjunction with Fig. 1, a novel anaerobic culture dish comprises a culture dish body 1 and a cover body 2, the culture dish body 1 and the lid body 2 above it are connected by threads to form a closed cavity, and the culture dish body 1 and the cover body 2 are made of transparent glass. The outer wall of the opening end of the culture dish body 1 is provided with an external thread 3 , and the inner wall of the opening end of the cover body 2 is provided with an internal thread 4 , and the external thread 3 is compatible with the internal thread 4 . An annular mounting seat 5 is provided on the inner side wall of the cover body 1 , and a rubber sealing ring 6 is disposed on a side of the mounting seat 5 close to the internal thread 4 . One side of the top of the cover body 2 is provided with an air inlet pipe 7, one end of the air inlet pipe 7 communicates with the inside of the culture dish body 1, and the other end can be connected with a nitrogen cylinder. A gas filter 8 is arranged on the air intake pipe 7, and the gas filter 8 is preferably a double-layer filter with a membrane filter layer and activated carbon.

进气管7上还配置有第一闸阀9，第一闸阀9控制氮气经进气管7进入培养皿本体1和盖体2形成的密闭空腔，所述第一闸阀9位于气体过滤器8和盖体2之间。所述盖体2顶部的另一侧设置有出气管10，出气管10也由透明玻璃制成，出气管10的一端也与培养皿本体1的内部相通，另一端与外界相通，出气管10由竖向管体和横向管体连接而成，横向管体与盖体2的顶部平行。出气管10上具有用于放置氧气指示剂的球形观测腔11，所述球形观测腔11位于横向管体内，球形观测腔11的内径大于横向管体的内径。所述第二闸阀12控制培养皿本体1和盖体2形成的密闭空腔内的气体，经由出气管10排到外界。The inlet pipe 7 is also equipped with a first gate valve 9, the first gate valve 9 controls nitrogen to enter the airtight cavity formed by the culture dish body 1 and the cover body 2 through the inlet pipe 7, and the first gate valve 9 is located between the gas filter 8 and the cover. between body 2. The other side of the top of the cover body 2 is provided with an air outlet pipe 10, and the air outlet pipe 10 is also made of transparent glass. One end of the air outlet pipe 10 also communicates with the inside of the culture dish body 1, and the other end communicates with the outside world. It is formed by connecting a vertical pipe body and a horizontal pipe body, and the horizontal pipe body is parallel to the top of the cover body 2 . The air outlet pipe 10 has a spherical observation cavity 11 for placing an oxygen indicator, the spherical observation cavity 11 is located in the transverse tube body, and the inner diameter of the spherical observation cavity 11 is larger than the inner diameter of the transverse tube body. The second gate valve 12 controls the gas in the airtight cavity formed by the culture dish body 1 and the cover 2 to be discharged to the outside through the air outlet pipe 10 .

首先，将第一闸阀9、气体过滤器8与进气管7组装在一起，将第二闸阀12与带有球形观测腔11的出气管10组装在一起，再将进气管7和出气管10分别连接到盖体2的顶部，同时，向球形观测腔11内加入氧气指示剂。在培养皿本体1内放置厌氧菌，将盖体2与培养皿本体1拧紧密封，打开第一闸阀9，经气体过滤器8过滤后的氮气进入培养皿本体1内，同时打开第二闸阀12，培养皿本体1内的混合气体，经出气管10排到外界，在其过程中培养皿本体1氧气逐渐降低，当氧气指示剂变成粉红色的时候，培养皿本体1内的氧气含量已下降到足够低的水平，能满足厌氧菌的生长，同时关闭第一闸阀9和第二闸阀12，保持培养皿本体1密封。First, assemble the first gate valve 9, the gas filter 8 and the air inlet pipe 7, assemble the second gate valve 12 and the air outlet pipe 10 with the spherical observation cavity 11, and then assemble the air inlet pipe 7 and the air outlet pipe 10 respectively It is connected to the top of the cover body 2, and at the same time, an oxygen indicator is added into the spherical observation chamber 11. Place anaerobic bacteria in the petri dish body 1, tighten the lid 2 and the petri dish body 1 tightly, open the first gate valve 9, nitrogen gas filtered by the gas filter 8 enters the petri dish body 1, and open the second gate valve at the same time 12. The mixed gas in the petri dish body 1 is discharged to the outside through the outlet pipe 10. During the process, the oxygen in the petri dish body 1 gradually decreases. When the oxygen indicator turns pink, the oxygen content in the petri dish body 1 has dropped to a low enough level to satisfy the growth of anaerobic bacteria, and at the same time close the first gate valve 9 and the second gate valve 12 to keep the petri dish body 1 sealed.

当然，上述说明并非是对本发明的限制，本发明也并不仅限于上述举例，本技术领域的技术人员在本发明的实质范围内所做出的变化、改型、添加或替换，也应属于本发明的保护范围。Of course, the above descriptions are not intended to limit the present invention, and the present invention is not limited to the above examples. Changes, modifications, additions or replacements made by those skilled in the art within the scope of the present invention shall also belong to the present invention. protection scope of the invention.

Claims (7)

1. a Novel anaerobic bacterium culture dish, comprises culture dish body and lid, it is characterized in that, described culture dish body and the lid above it are threaded connection, and form airtight cavity; The side at described lid top is provided with inlet pipe, and inlet pipe is configured with gas filter, and inlet pipe is also configured with the first gate valve, and described first gate valve is between gas filter and lid; The opposite side at described lid top is provided with escape pipe, escape pipe has the spherical observation chamber for placing oxygen indicator, the escape pipe between described spherical observation chamber and lid is provided with the second gate valve.
2. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterized in that, one end of described inlet pipe communicates with the inside of culture dish body, and the other end can connect nitrogengas cylinder; One end of described escape pipe also communicates with the inside of culture dish body, and the other end communicates with the external world.
3. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterized in that, described gas filter is the bilayer filter with membrane filtration layer and gac.
4. a kind of Novel anaerobic bacterium culture dish according to claim 1, is characterized in that, the outer side wall of described culture dish body open end is provided with outside screw, the inner side-wall of lid opening end is provided with internal thread, outside screw and internal thread suitable.
5. a kind of Novel anaerobic bacterium culture dish according to claim 1, is characterized in that, the inner side-wall of described lid is provided with the mount pad of annular, mount pad is configured with rubber seal near the side of internal thread.
6. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterized in that, described culture dish body and lid are made by transparent glass, and escape pipe is also made up of transparent glass.
7. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterized in that, described escape pipe is formed by connecting by vertical body and horizontal body, and horizontal body is parallel with the top of lid; Described spherical observation chamber is positioned at horizontal body, and the internal diameter in spherical observation chamber is greater than the internal diameter of horizontal body.
CN201510966817.9A 2015-12-21 2015-12-21 Novel anaerobic-bacterium culturing dish Pending CN105385582A (en)

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Application publication date: 20160309