CN105385582A - Novel anaerobic-bacterium culturing dish - Google Patents
Novel anaerobic-bacterium culturing dish Download PDFInfo
- Publication number
- CN105385582A CN105385582A CN201510966817.9A CN201510966817A CN105385582A CN 105385582 A CN105385582 A CN 105385582A CN 201510966817 A CN201510966817 A CN 201510966817A CN 105385582 A CN105385582 A CN 105385582A
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- China
- Prior art keywords
- culture dish
- lid
- novel anaerobic
- escape pipe
- anaerobic bacterium
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
- C12M37/04—Seals
Abstract
The invention discloses a novel anaerobic-bacterium culturing dish. The novel anaerobic-bacterium culturing dish comprises a culturing dish body and a cover. The culturing dish body is connected with the cover on the culturing dish body through threads to form an airtight cavity. A gas inlet pipe is arranged on one side of the top of the cover and provided with a gas filter. A first gate valve is further arranged on the gas inlet pipe and located between the gas filter and the cover. A gas outlet pipe is arranged on the other side of the top of the cover and provided with a spherical observing cavity for containing an oxygen indicator, and a second gate valve is arranged at the portion, between the spherical observing cavity and the cover, of the gas outlet pipe. The novel anaerobic-bacterium culturing dish is simple in structure, high in practicability, low in manufacturing cost, easy to operate and convenient to widely use and popularize.
Description
Technical field
The present invention relates to geomicrobiology experimental implementation field, be specifically related to a kind of Novel anaerobic bacterium culture dish.
Background technology
Culture dish, is generally used for the cultivation of animal and plant cells or microorganism cells, and be often made up of glass or plastics, structure is simple.Technical improvement about culture dish is more, except routine can except the glass culture dish possessing different diameter of sterilizing, the culture dish of one more.But these culture dish mostly utilize aerobic environment to carry out cell cultures.Be specifically designed to the culture dish cultivating anaerobion at present less.
Cultivate anerobe, must create the environment of an anaerobic, some anerobe even needs strict oxygen-free environment.Add reductive agent in usual substratum, or remove the free oxygen in environment by physics, chemical process, to reduce redox-potential.Conventional anaerobic bacteria culture method has many, the methods such as such as layer plating method, anaerobism work box culture method, Anaerobicbag, biological oxygen consumption.Above-mentioned cultural method complicated operation, some method anaerobism effect is undesirable.In order to cultivate anaerobion more easily, being necessary to make a kind of culture dish being specifically designed to cultivation anerobe, requiring that this culture dish has good obturation effect, can a kind of anaerobic environment be created, simultaneously the simple easy handling of culturing process.
Summary of the invention
For above-mentioned the deficiencies in the prior art, the invention provides a kind of Novel anaerobic bacterium culture dish, the features such as this culture dish possesses good sealing effect, can provide anaerobic environment, simple to operate.
In order to solve the problems of the technologies described above, the technical solution adopted in the present invention is:
A kind of Novel anaerobic bacterium culture dish, comprise culture dish body and lid, described culture dish body and the lid above it are threaded connection, and form airtight cavity.The side at described lid top is provided with inlet pipe, and inlet pipe is configured with gas filter, and inlet pipe is also configured with the first gate valve, and described first gate valve is between gas filter and lid.The opposite side at described lid top is provided with escape pipe, escape pipe has the spherical observation chamber for placing oxygen indicator, the escape pipe between described spherical observation chamber and lid is provided with the second gate valve.
Preferably, one end of described inlet pipe communicates with the inside of culture dish body, and the other end can connect nitrogen gas cylinder.One end of described escape pipe also communicates with the inside of culture dish body, and the other end communicates with the external world.
Preferably, described gas filter is the bilayer filter with membrane filtration layer and gac.
Preferably, the outer side wall of described culture dish body open end is provided with outside screw, the inner side-wall of lid opening end is provided with internal thread, outside screw and internal thread suitable.
Preferably, the inner side-wall of described lid is provided with the mount pad of annular, mount pad is configured with rubber seal near the side of internal thread.
Preferably, described culture dish body and lid are made by transparent glass, and escape pipe is also made up of transparent glass.
Preferably, described escape pipe is formed by connecting by vertical body and horizontal body, and horizontal body is parallel with the top of lid.Described spherical observation chamber is positioned at horizontal body, and the internal diameter in spherical observation chamber is greater than the internal diameter of horizontal body.
By adopting technique scheme, Advantageous Effects of the present invention is: due to outside screw and internal thread suitable, and sealing-ring is set in lid, culture dish obturation effect can be ensured, be filled with nitrogen through inlet pipe, original gas in culture dish discharged by vapor pipe, and the oxygen content in culture dish is declined, by the colour-change of the oxygen indicator in spherical observation chamber, can illustrate whether the oxygen content in culture dish reaches the requirement of anerobe growth.Structure of the present invention is simple, and practical, low cost of manufacture, easy handling, is convenient to extensively promote the use of.
Accompanying drawing explanation
Fig. 1 is the structural principle schematic diagram of a kind of Novel anaerobic bacterium of the present invention culture dish.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in detail:
Composition graphs 1, a kind of Novel anaerobic bacterium culture dish, comprises culture dish body 1 and lid 2, and described culture dish body 1 is threaded connection with the lid 2 above it, and form airtight cavity, described culture dish body 1 and lid 2 are made by transparent glass.The outer side wall of described culture dish body 1 opening end is provided with outside screw 3, the inner side-wall of lid 2 opening end is provided with internal thread 4, described outside screw 3 is suitable with internal thread 4.The inner side-wall of described lid 1 is provided with the mount pad 5 of annular, mount pad 5 is configured with rubber seal 6 near the side of internal thread 4.The side at described lid 2 top is provided with inlet pipe 7, and one end of inlet pipe 7 communicates with the inside of culture dish body 1, and the other end can connect nitrogen gas cylinder.Inlet pipe 7 is configured with gas filter 8, described gas filter 8 preferably has the bilayer filter of membrane filtration layer and gac.
Inlet pipe 7 is also configured with the first gate valve 9, first gate valve 9 and controls nitrogen enters culture dish body 1 and lid 2 formation closed cavity through inlet pipe 7, described first gate valve 9 is between gas filter 8 and lid 2.The opposite side at described lid 2 top is provided with escape pipe 10, escape pipe 10 is also made up of transparent glass, one end of escape pipe 10 also communicates with the inside of culture dish body 1, the other end communicates with the external world, escape pipe 10 is formed by connecting by vertical body and horizontal body, and horizontal body is parallel with the top of lid 2.Escape pipe 10 has the spherical observation chamber 11 for placing oxygen indicator, described spherical observation chamber 11 is positioned at horizontal body, and the internal diameter in spherical observation chamber 11 is greater than the internal diameter of horizontal body.Described second gate valve 12 controls the gas in the closed cavity of culture dish body 1 and lid 2 formation, is discharged to the external world via escape pipe 10.
First, first gate valve 9, gas filter 8 are fitted together with inlet pipe 7, second gate valve 12 is fitted together with the escape pipe 10 with spherical observation chamber 11, again inlet pipe 7 and escape pipe 10 are connected respectively to the top of lid 2, meanwhile, in spherical observation chamber 11, oxygen indicator is added.Anerobe is placed in culture dish body 1, lid 2 and culture dish body 1 are tightened sealing, open the first gate valve 9, nitrogen after gas filter 8 filters enters in culture dish body 1, open the second gate valve 12 simultaneously, mixed gas in culture dish body 1, the external world is discharged to through escape pipe 10, in its process, culture dish body 1 oxygen reduces gradually, when oxygen indicator becomes peach time, oxygen content in culture dish body 1 has dropped to enough low level, the growth of anerobe can be met, close the first gate valve 9 and the second gate valve 12 simultaneously, culture dish body 1 is kept to seal.
Certainly, above-mentioned explanation is not limitation of the present invention, and the present invention is also not limited in above-mentioned citing, and the change that those skilled in the art make in essential scope of the present invention, remodeling, interpolation or replacement also should belong to protection scope of the present invention.
Claims (7)
1. a Novel anaerobic bacterium culture dish, comprises culture dish body and lid, it is characterized in that, described culture dish body and the lid above it are threaded connection, and form airtight cavity; The side at described lid top is provided with inlet pipe, and inlet pipe is configured with gas filter, and inlet pipe is also configured with the first gate valve, and described first gate valve is between gas filter and lid; The opposite side at described lid top is provided with escape pipe, escape pipe has the spherical observation chamber for placing oxygen indicator, the escape pipe between described spherical observation chamber and lid is provided with the second gate valve.
2. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterized in that, one end of described inlet pipe communicates with the inside of culture dish body, and the other end can connect nitrogengas cylinder; One end of described escape pipe also communicates with the inside of culture dish body, and the other end communicates with the external world.
3. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterized in that, described gas filter is the bilayer filter with membrane filtration layer and gac.
4. a kind of Novel anaerobic bacterium culture dish according to claim 1, is characterized in that, the outer side wall of described culture dish body open end is provided with outside screw, the inner side-wall of lid opening end is provided with internal thread, outside screw and internal thread suitable.
5. a kind of Novel anaerobic bacterium culture dish according to claim 1, is characterized in that, the inner side-wall of described lid is provided with the mount pad of annular, mount pad is configured with rubber seal near the side of internal thread.
6. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterized in that, described culture dish body and lid are made by transparent glass, and escape pipe is also made up of transparent glass.
7. a kind of Novel anaerobic bacterium culture dish according to claim 1, it is characterized in that, described escape pipe is formed by connecting by vertical body and horizontal body, and horizontal body is parallel with the top of lid; Described spherical observation chamber is positioned at horizontal body, and the internal diameter in spherical observation chamber is greater than the internal diameter of horizontal body.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510966817.9A CN105385582A (en) | 2015-12-21 | 2015-12-21 | Novel anaerobic-bacterium culturing dish |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510966817.9A CN105385582A (en) | 2015-12-21 | 2015-12-21 | Novel anaerobic-bacterium culturing dish |
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| CN105385582A true CN105385582A (en) | 2016-03-09 |
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| CN201510966817.9A Pending CN105385582A (en) | 2015-12-21 | 2015-12-21 | Novel anaerobic-bacterium culturing dish |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967591A (en) * | 2017-06-01 | 2017-07-21 | 田金静 | A kind of clinical microbiology anaerobe samples storage bottle |
| CN109022256A (en) * | 2018-07-17 | 2018-12-18 | 广东药科大学附属第医院 | Anaerobic culturel bag with Anaerobic indicator |
| CN111269821A (en) * | 2020-03-24 | 2020-06-12 | 宁波大学 | Microorganism culture device and method |
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| CN200971362Y (en) * | 2006-11-30 | 2007-11-07 | 王军 | Enclosed bacterial culture dish |
| CN201969447U (en) * | 2011-03-21 | 2011-09-14 | 贵州东仪医疗器械有限公司 | Gas purification filter |
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| CN104066833A (en) * | 2011-12-06 | 2014-09-24 | 医用蛋白国际有限责任公司 | Closed bioreactor |
| CN204251599U (en) * | 2014-09-17 | 2015-04-08 | 中牧实业股份有限公司 | A kind of anaerobic microorganism culturing device |
| CN204400992U (en) * | 2015-01-13 | 2015-06-17 | 贵州省分析测试研究院 | Degrading experiment culturing bottle |
| CN205275598U (en) * | 2015-12-21 | 2016-06-01 | 山东科技大学 | Novel anaerobe culture ware |
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2015
- 2015-12-21 CN CN201510966817.9A patent/CN105385582A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN200971362Y (en) * | 2006-11-30 | 2007-11-07 | 王军 | Enclosed bacterial culture dish |
| CN201969447U (en) * | 2011-03-21 | 2011-09-14 | 贵州东仪医疗器械有限公司 | Gas purification filter |
| CN202246703U (en) * | 2011-10-09 | 2012-05-30 | 黑龙江八一农垦大学 | Cell culture bottle capable of being filled with nitrogen |
| CN104066833A (en) * | 2011-12-06 | 2014-09-24 | 医用蛋白国际有限责任公司 | Closed bioreactor |
| CN103045475A (en) * | 2012-12-04 | 2013-04-17 | 湖北民族学院 | Air-lift biochemical culture apparatus |
| CN204251599U (en) * | 2014-09-17 | 2015-04-08 | 中牧实业股份有限公司 | A kind of anaerobic microorganism culturing device |
| CN204400992U (en) * | 2015-01-13 | 2015-06-17 | 贵州省分析测试研究院 | Degrading experiment culturing bottle |
| CN205275598U (en) * | 2015-12-21 | 2016-06-01 | 山东科技大学 | Novel anaerobe culture ware |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106967591A (en) * | 2017-06-01 | 2017-07-21 | 田金静 | A kind of clinical microbiology anaerobe samples storage bottle |
| CN106967591B (en) * | 2017-06-01 | 2019-07-09 | 田金静 | A kind of clinical microbiology anaerobe samples storage bottle |
| CN109022256A (en) * | 2018-07-17 | 2018-12-18 | 广东药科大学附属第医院 | Anaerobic culturel bag with Anaerobic indicator |
| CN109022256B (en) * | 2018-07-17 | 2022-05-13 | 广东药科大学附属第一医院 | Anaerobic culture bag with anaerobic indicator |
| CN111269821A (en) * | 2020-03-24 | 2020-06-12 | 宁波大学 | Microorganism culture device and method |
| CN111269821B (en) * | 2020-03-24 | 2023-12-19 | 宁波大学 | Microorganism culture device and method |
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Application publication date: 20160309 |
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