CN203048956U - Virus plaque culture box - Google Patents

Virus plaque culture box Download PDF

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Publication number
CN203048956U
CN203048956U CN 201320085065 CN201320085065U CN203048956U CN 203048956 U CN203048956 U CN 203048956U CN 201320085065 CN201320085065 CN 201320085065 CN 201320085065 U CN201320085065 U CN 201320085065U CN 203048956 U CN203048956 U CN 203048956U
Authority
CN
China
Prior art keywords
box
box body
virus
cotton
dividing plate
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
CN 201320085065
Other languages
Chinese (zh)
Inventor
徐黎明
卢彤岩
刘红柏
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Heilongjiang Institute of Fisheries Chinese Academy of Fishery Sciences
Original Assignee
Heilongjiang Institute of Fisheries Chinese Academy of Fishery Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Heilongjiang Institute of Fisheries Chinese Academy of Fishery Sciences filed Critical Heilongjiang Institute of Fisheries Chinese Academy of Fishery Sciences
Priority to CN 201320085065 priority Critical patent/CN203048956U/en
Application granted granted Critical
Publication of CN203048956U publication Critical patent/CN203048956U/en
Expired - Fee Related legal-status Critical Current
Anticipated expiration legal-status Critical

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Abstract

The utility model provides a virus plaque culture box, and belongs to the field of virus research, solving the problems in the prior art that a low-temperature CO2 cell culture box is needed during purifying the virus plaque which grows at temperature below 20 DEG C, and relative high requirements are brought to the equipment, simultaneously, a cell culture plate is poor in tightness, therefore, the humidity in the cell culture plate is easily reduced, which influences the formation of the virus plaque and even easily enables the virus to spread to pollute the cell culture box. The culture box comprises a seal box, a partition plate and a moisturizing cotton; the seal box consists of a box body and a box cover; the box cover is covered on the box, and the part where the box cover is connected with the box body is sealed; the partition plate and the moisturizing cotton are arranged in the box body; the moisturizing cotton is placed at the bottom part of the box body; the partition plate is arranged on the moisturizing cotton, with four side surfaces fixed onto the box body; and a plurality of flow holes which pass through the partition plate are formed in the partition plate. The virus plaque culture box is used for culturing the virus plaque.

Description

A kind of viral plaque is cultivated box
Technical field
The utility model relates to a kind of viral plaque and cultivates box, belongs to the virus research field.
Background technology
The most basic requirement of experiment that the separation of virus and purifying are virus research, and viral plaque purification is the most frequently used viral purification methods.The formation of virus plaque needs the microenvironment that parameters such as temperature, humidity and potential of hydrogen adapt.At present method commonly used is that six orifice plates with virus inoculation directly are inverted in the CO2 cell culture incubator and cultivate, but because the different virus growth is to the requirement difference of temperature, some viral growth temperature is below 20 ℃, if adopt the low temperature incubator that does not have CO2, owing to there is not CO2, in viral plaque purification process, be difficult to guarantee substratum potential of hydrogen in the Tissue Culture Plate, therefore, need in low temperature CO2 cell culture incubator, cultivate, but existing low temperature CO2 cell culture incubator involves great expense, popularity rate is lower, the stopping property of Tissue Culture Plate is relatively poor simultaneously, causes humidity reduction in the Tissue Culture Plate, influences the formation of viral plaque, and cause the virus diffusion easily, the contamination of cells incubator.
The utility model content
The utility model is for solving existing growth temperature in the process of the viral plaque purification below 20 ℃, need to use low temperature CO2 cell culture incubator, to having relatively high expectations of equipment, simultaneously, the stopping property of Tissue Culture Plate is relatively poor, easily causes humidity reduction in the Tissue Culture Plate, influences the formation of viral plaque, and cause the virus diffusion easily, the problem of contamination of cells incubator.
Provide a kind of viral plaque to cultivate box for solving the problems of the technologies described above, described cultivation box comprises seal box, dividing plate and the cotton of preserving moisture, described seal box comprises box body and lid, lid covers on box body and the two junction sealing, dividing plate and the cotton of preserving moisture are arranged on the inside of box body, the cotton of preserving moisture is positioned over the bottom of box body, and the side all around that dividing plate is arranged on preserve moisture cotton top and dividing plate is fixed on the box body, has the opening of a plurality of perforation dividing plates on the described dividing plate.
The utility model compared with prior art has following beneficial effect: seal box described in the utility model has good sealing property, can prevent that virus from polluting incubator, simultaneously, before experiment, the cell culture fluid potential of hydrogen in the seal box adjusted and adjust humidity in the seal box by the cotton of preserving moisture, after being positioned over substratum on the dividing plate, seal box is sealed, can effectively guarantee the humidity of substratum in the experimentation, select the common biochemical incubator that is fit to the viral living environment of cultivating for use, seal box is positioned over breeding and the amplification of carrying out virus in the selected common biochemical incubator, for the formation of viral plaque provides a good microenvironment, and do not need to use low temperature CO2 cell culture incubator, reduced the requirement to equipment.
Description of drawings
Fig. 1 is sectional view of the present utility model, and Fig. 2 is external structure of the present utility model, and Fig. 3 is the structural representation of dividing plate 1.
Embodiment
Embodiment one: present embodiment is described in conjunction with Fig. 1, Fig. 2 and Fig. 3, the described a kind of viral plaque of present embodiment is cultivated box, described cultivation box comprises seal box, dividing plate 1 and preserves moisture cotton 2, described seal box comprises box body 3 and lid 4, lid 4 covers on box body 3 and the two junction sealing, dividing plate 1 and the cotton 2 of preserving moisture are arranged on the inside of box body 3, the cotton 2 of preserving moisture is positioned over the bottom of box body 3, dividing plate 1 is arranged on the top of the cotton 2 of preserving moisture and the side all around of dividing plate 1 is fixed on the box body 3, has the opening 5 of a plurality of perforation dividing plates 1 on the described dividing plate 1.
Embodiment two: in conjunction with Fig. 1 present embodiment is described, the described dividing plate 1 of present embodiment is horizontally set in the box body 3, so arranges, and more stable after making substratum be positioned on the dividing plate 1, other compositions are identical with embodiment one with annexation.
Principle of work:
The utility model in use, at first will inoculate and put into the aseptic seal box that passes through uv sterilisation after virulent substratum is adjusted potential of hydrogen, substratum is positioned on the dividing plate 1, the cotton 2 of preserving moisture is aseptic cotton, according to different virus humidity required degree to adjust to preserve moisture in the seal box cotton 2 humidity, with the seal box good seal, select the common biochemical incubator that is fit to the viral living environment of cultivating for use, seal box is positioned over breeding and the amplification of carrying out virus in the selected common biochemical incubator.

Claims (2)

1. a viral plaque is cultivated box, it is characterized in that: described cultivation box comprises seal box, dividing plate (1) and the cotton of preserving moisture (2), described seal box comprises box body (3) and lid (4), lid (4) covers at box body (3) to be gone up and the two junction sealing, dividing plate (1) and the cotton of preserving moisture (2) are arranged on the inside of box body (3), the cotton (2) of preserving moisture is positioned over the bottom of box body (3), dividing plate (1) is arranged on the top of the cotton of preserving moisture (2) and the side all around of dividing plate (1) is fixed on the box body (3), has the opening (5) of a plurality of perforation dividing plates (1) on the described dividing plate (1).
2. cultivate box according to the described a kind of viral plaque of claim 1, it is characterized in that: described dividing plate (1) is horizontally set in the box body (3).
CN 201320085065 2013-02-25 2013-02-25 Virus plaque culture box Expired - Fee Related CN203048956U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN 201320085065 CN203048956U (en) 2013-02-25 2013-02-25 Virus plaque culture box

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN 201320085065 CN203048956U (en) 2013-02-25 2013-02-25 Virus plaque culture box

Publications (1)

Publication Number Publication Date
CN203048956U true CN203048956U (en) 2013-07-10

Family

ID=48731933

Family Applications (1)

Application Number Title Priority Date Filing Date
CN 201320085065 Expired - Fee Related CN203048956U (en) 2013-02-25 2013-02-25 Virus plaque culture box

Country Status (1)

Country Link
CN (1) CN203048956U (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104480010A (en) * 2014-11-28 2015-04-01 清华大学 Biological reaction device and application thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104480010A (en) * 2014-11-28 2015-04-01 清华大学 Biological reaction device and application thereof

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Legal Events

Date Code Title Description
C14 Grant of patent or utility model
GR01 Patent grant
C17 Cessation of patent right
CF01 Termination of patent right due to non-payment of annual fee

Granted publication date: 20130710

Termination date: 20140225