CN204803326U - Cell culture changes bottle - Google Patents

Abstract

Cell culture changes bottle, including have the open -ended bottle, with the open end fixed connection's of bottle bottleneck and bottle lid, the surface of bottleneck is provided with the external screw thread, the bottle lid is including being used for the circumferential lateral wall of lower surface that seals bottle open -ended roof and be located the roof, be provided with on the lateral wall with external screw thread assorted internal thread, the lower surface laminating of roof has a ventilated membrane, and a plurality of bleeder vents have been seted up to the position that corresponds the ventilated membrane on the roof. The utility model discloses a cell culture changes bottle, bottle lid and bottleneck screw -thread fit for the bottle lid can be locked on the bottle, sets up simultaneously bleeder vent and ventilated membrane on the bottle lid, prevents at the bottle lid of guaranteeing to screw that the culture solution from leaking hunting, making when preventing cross contamination the gas can come in and go out in the bottle, and culture cell can the normal growth.

Description

Cell-culturing rotating bottle

Technical field

The utility model relates to cell co-culture tool technical field, is specifically related to a kind of cell-culturing rotating bottle.

Background technology

When carrying out cell cultures, zooblast needs continuous oxygen gas-supplying and gets rid of carbonic acid gas, vegetable cell then needs constantly to supply carbonic acid gas and gets rid of oxygen, and thus Tissue Culture Flask is to the supply of oxygen or carbonic acid gas, and the discharge of carbonic acid gas or oxygen is extremely important.In existing Tissue Culture Flask, mainly comprise body, bottleneck and bottle cap.When but Tissue Culture Flask of the prior art needs ventilative, generally by unclamping bottle cap, gap is reserved in the junction of pipe lid and bottleneck, thus meeting ventilative requirement, this mode, is difficult to the elasticity getting hold of bottle cap, generally after screwing, half-turn is unscrewed again for degree during open culture, but often air permeable effect is unstable, and the Medium's PH Value in cell bottle and osmotic pressure cannot maintain perfect condition, if the pine excessively more seriously twisted causes nutrient solution to spill or crossed contamination; And during airtight cultivation, lid tension makes gas exchange, accelerate to break acid base equilibrium in culture system, meanwhile, cause screw socket stripped thread, destroy the stopping property of culturing bottle.

Utility model content

For the deficiencies in the prior art, the purpose of this utility model is to provide a kind of cell-culturing rotating bottle, and air permeable effect is good, and structure is simple.

For achieving the above object, the utility model adopts following technical scheme:

Cell-culturing rotating bottle, comprise the bottle with opening, the bottleneck be fixedly connected with the opening end of bottle and bottle cap, the outside surface of bottleneck is provided with outside screw; Bottle cap comprise for closed bottle opening roof and be positioned at the sidewall of lower surface periphery of roof, sidewall is provided with the internal thread matched with outside screw; The lower surface of roof is fitted with an air-permeable envelope, and on roof, the position of corresponding air-permeable envelope offers multiple breather hole.

Preferably, multiple breather hole is along the central axis circumference array of roof.

Preferably, breather hole is provided with six.

Preferably, the aperture of breather hole is 5mm.

The beneficial effects of the utility model are:

Compared to prior art, cell-culturing rotating bottle of the present utility model, bottle cap coordinates with bottleneck thread, bottle cap can be locked on bottle, breather hole and air-permeable envelope are set on bottle cap simultaneously, guarantee screw bottle cap prevent nutrient solution from leaking hunting, prevent crossed contamination while gas can be come in and gone out in bottle, culturing cell can normal growth.

Accompanying drawing explanation

Fig. 1 is the structural representation of bottle of the present utility model;

Fig. 2 is the structural representation of bottle cap of the present utility model;

Fig. 3 is the cross section structure schematic diagram of bottle cap of the present utility model.

Wherein, 10, bottle; 20, bottleneck; 21, outside screw; 30, bottle cap; 31, internal thread; 32, breather hole; 33, air-permeable envelope.

Embodiment

Below, by reference to the accompanying drawings and embodiment, the utility model is described further:

As Fig. 1,2 and Fig. 3 shown in cell-culturing rotating bottle, comprise and have bottle 10, bottleneck 20 and bottle cap 30, this bottle 10 one end has opening, conveniently puts into cell culture fluid.Bottleneck 20 is fixedly connected on one end that bottle 10 has opening, at the outside surface of this bottleneck 20, outside screw 21 is set, the internal thread 31 matched with outside screw 21 is set at above-mentioned bottle cap 30 internal surface accordingly, bottle cap 30 is threaded with the opening of closed bottle 10 with bottleneck 20, so contributes to the Growth of Cells in bottle 10.

Above-mentioned bottle cap 30 comprises roof for closed bottle 10 opening and sidewall, and this sidewall is positioned at the lower surface periphery of roof, is specifically arranged on the internal surface of this sidewall at above-mentioned internal thread 31.In addition, an air-permeable envelope 33 is fitted with at the lower surface of roof, simultaneously on roof to the position of air-permeable envelope 33 offering multiple breather hole 32, enable aseptic gas via this breather hole 32 can through entering in bottle 10 under the effect of air-permeable envelope 33, ensure the circulation of the gas in bottle 10, make culturing cell in bottle 10 can normal growth; And the iris action that air-permeable envelope 33 has makes bottle 10 inner cell sample not leak, and other impurity outside bottle 10 also cannot enter in bottle 10, and the cell culture environment in bottle 10 is better.

Preferably, above-mentioned multiple breather hole 32 can, along the central axis circumference array of roof, can make gas to enter more uniformly in bottle 10, and the gas volume that the cell of bottle 10 is obtained is consistent, and production rate and degree are consistent.Further, in the present embodiment, above-mentioned breather hole 32 specifically can arrange six, and meanwhile, the aperture making breather hole 32 is 5mm, ensures the discrepancy amount of rational gas.

Cell-culturing rotating bottle of the present utility model, bottle cap 30 and bottleneck 20 thread fit, make bottle cap 30 can be locked on bottle 10, breather hole 32 and air-permeable envelope 33 are set on bottle cap 30 simultaneously, guarantee screw bottle cap prevent nutrient solution from leaking hunting, prevent crossed contamination while gas can be come in and gone out in bottle, culturing cell can normal growth.

To one skilled in the art, according to technical scheme described above and design, other various corresponding change and deformation can be made, and all these change and deformation all should belong within the protection domain of the utility model claim.

Claims (4)

1. cell-culturing rotating bottle, comprise the bottle with opening, the bottleneck be fixedly connected with the opening end of bottle and bottle cap, it is characterized in that, the outside surface of bottleneck is provided with outside screw; Bottle cap comprise for closed bottle opening roof and be positioned at the sidewall of lower surface periphery of roof, sidewall is provided with the internal thread matched with outside screw; The lower surface of roof is fitted with an air-permeable envelope, and on roof, the position of corresponding air-permeable envelope offers multiple breather hole.

2. cell-culturing rotating bottle as claimed in claim 1, it is characterized in that, multiple breather hole is along the central axis circumference array of roof.

3. cell-culturing rotating bottle as claimed in claim 2, it is characterized in that, breather hole is provided with six.

4. cell-culturing rotating bottle as claimed in claim 2, it is characterized in that, the aperture of breather hole is 5mm.