CN113061521A - High-effect cell culture tube - Google Patents
High-effect cell culture tube Download PDFInfo
- Publication number
- CN113061521A CN113061521A CN202110364783.1A CN202110364783A CN113061521A CN 113061521 A CN113061521 A CN 113061521A CN 202110364783 A CN202110364783 A CN 202110364783A CN 113061521 A CN113061521 A CN 113061521A
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- China
- Prior art keywords
- culture tube
- culture
- tube body
- cell culture
- cultivation frame
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- 238000004113 cell culture Methods 0.000 title claims abstract description 23
- 239000012528 membrane Substances 0.000 claims abstract description 9
- 238000000034 method Methods 0.000 abstract description 8
- 230000008569 process Effects 0.000 abstract description 8
- 238000012360 testing method Methods 0.000 abstract description 7
- 238000011161 development Methods 0.000 abstract description 4
- 238000012827 research and development Methods 0.000 abstract description 4
- 230000000052 comparative effect Effects 0.000 abstract description 2
- 238000002474 experimental method Methods 0.000 abstract description 2
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 238000009423 ventilation Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 abstract 1
- 238000007789 sealing Methods 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 239000011148 porous material Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000012795 verification Methods 0.000 description 1
Images
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/06—Tubular
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/38—Caps; Covers; Plugs; Pouring means
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
Abstract
The invention discloses a high-efficiency cell culture tube, which comprises a culture tube cultivation frame and is characterized in that a plurality of culture tubes which are uniformly inserted into the top wall of the culture tube cultivation frame are arranged on the culture tube cultivation frame; every the culture tube includes the culture tube body, wherein is equipped with the fixed cover of culture tube on culture tube body circumference wall top, is equipped with the culture tube lid at the fixed overcoat of culture tube, is equipped with the culture tube ventilated membrane at culture tube body inside wall, supports through the supporting network between the bottom of culture tube ventilated membrane and culture tube body, is equipped with filter paper on culture tube lid roof. The invention greatly improves the efficiency and effectiveness of cell culture in the process of principle research and development; the high-efficiency ventilation effect greatly improves the growth speed and the growth density of cells and shortens the test period; the closed bottleneck structure effectively prevents infection in the cell culture process in the research and development process; the use of an array of culture tubes 7 greatly provides the efficacy of comparative experiments in the development process.
Description
Technical Field
The invention relates to the technical field of cell culture equipment, in particular to a high-efficiency cell culture tube.
Background
The preparation of biological medicines is in a rapid development stage at present, and a high-efficiency small-capacity culture container is needed for rapid principle and feasibility verification. In the traditional cell culture process, various culture pore plates are used, the cell culture amount is very limited, and the dosage for researches such as immune cell therapy and the like is difficult to meet. And the number of holes of the traditional pore plate is more, only partial hole sites are used in the test, and a large amount of residual hole sites are wasted. At present, the cell culture consumables with small capacity generally adopt an open cover plate, cannot effectively seal a sample, and easily generate the risk of sample pollution in the test process.
Disclosure of Invention
The present invention is directed to a high performance cell culture tube, which solves the above problems of the prior art.
In order to achieve the purpose, the invention provides the following technical scheme:
a high-efficiency cell culture tube comprises a culture tube cultivation frame, and is characterized in that a plurality of culture tubes are uniformly inserted into the top wall of the culture tube cultivation frame;
every the culture tube includes the culture tube body, wherein is equipped with the fixed cover of culture tube on culture tube body circumference wall top, is equipped with the culture tube lid at the fixed overcoat of culture tube, is equipped with the culture tube ventilated membrane at culture tube body inside wall, supports through the supporting network between the bottom of culture tube ventilated membrane and culture tube body, is equipped with filter paper on culture tube lid roof.
As a preferable scheme of the invention, the culture tube fixing sleeve is in threaded connection with the culture tube cover.
In a preferred embodiment of the present invention, the screw connection has a double-helical screw structure.
As the preferable scheme of the invention, a plurality of limiting bulges are arranged between the bottom of the inner side of the tube body of the culture tube and the supporting net.
In a preferred embodiment of the invention, two stop rings are arranged up and down between the breathable film of the culture tube and the inner side wall of the tube body of the culture tube.
Has the advantages that:
1. the single culture tube is integrally designed, so that pollution can be prevented;
2. the bottle cap is ventilated, and cells can grow rapidly;
3. the number of culture tubes can be selected according to the requirement;
4. the assembly has simple structure and convenient use.
Drawings
FIG. 1 is a perspective view of a high performance cell culture tube according to the present invention;
FIG. 2 is a cross-sectional view of a high performance cell culture tube according to the present invention.
In the figure: 1. filtering paper; 2. a culture tube cover; 3. fixing a culture tube sleeve; 4. a culture tube air-permeable membrane; 5. a culture tube body; 6. a support net; 7. a culture tube; 8. and (5) cultivating a tube planting frame.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention belong to the protection scope of the present invention.
To facilitate an understanding of the invention, the invention will now be described more fully with reference to the accompanying drawings. Several embodiments of the invention are presented in the drawings. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete.
It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.
Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.
Referring to fig. 1-2, the present invention provides a technical solution:
embodiment 1, please refer to fig. 1 and 2, a high performance cell culture tube includes a culture tube cultivation frame 8, wherein a plurality of culture tubes 7 are uniformly inserted into the top wall of the culture tube cultivation frame 8; each culture tube 7 comprises a culture tube body 5, wherein a culture tube fixing sleeve 3 is arranged at the top end of the circumferential wall of the culture tube body 5, a culture tube cover 2 is sleeved outside the culture tube fixing sleeve 3, a culture tube breathable film 4 is arranged on the inner side wall of the culture tube body 5, a support net 6 is used for supporting between the bottom of the culture tube breathable film 4 and the culture tube body 5, and filter paper 1 is arranged on the top wall of the culture tube cover 2; the culture tube fixing sleeve 3 is connected with the culture tube cover 2 through threads; the thread connection adopts a double-spiral thread structure; a plurality of limiting bulges are arranged between the bottom of the inner side of the culture tube body 5 and the supporting net 6; two limiting rings which are arranged up and down are arranged between the culture tube breathable film 4 and the inner side wall of the culture tube body 1.
The corresponding parts of the culture tube have the following functions,
filter paper 1: further increasing the gas exchange efficiency between the sample in the bottle and the incubator environment;
culture tube cap 2: the double-helix thread structure is adopted, so that the sealing performance of the tube body and the biological safety in the culture process are guaranteed;
fixing a culture tube sleeve 3: effectively fix the ventilated membrane tube body structure to the tube body main body. Meanwhile, the double-helix thread is effectively matched with the bottle cap, so that strict sealing performance is achieved;
culture tube ventilated membrane 4: the breathable film is packaged in a barrel in a one-step forming mode, so that the gas exchange requirement of a sample is met in a three-dimensional mode;
culture tube body 5: providing a product shell, and effectively protecting the inner tubular breathable film main body;
when carrying out high-effect cell culture research and development contrast experiment, can a plurality of culture tubes use simultaneously, improve contrast test efficiency greatly.
Example 2, in the present invention, the requirements for the various components can be selected as follows,
1. the culture tube 7 can be optimized to be any suitable size and any suitable shape according to requirements;
2. the culture tube 7 can be selected to be in any sealing mode according to requirements;
3. the culture tube breathable film 4 can be designed into any suitable size and shape according to the requirement, and different breathable materials are selected;
4. the culture tube cover 2 can be designed into any suitable shape according to the requirement;
5. the culture tube carrier 8 can be designed to be suitable for size, hole position and shape according to the requirement;
6. the number of culture tubes 7 in the parallel comparison test can be adjusted based on the use requirement.
In conclusion, 1, the invention greatly improves the efficiency and effectiveness of cell culture in the process of principle development;
2. the invention has high-efficiency ventilation efficiency, greatly improves the growth speed and the growth density of cells, and shortens the test period;
3. the closed bottleneck structure effectively prevents infection in the cell culture process in the research and development process;
4. the use of the array of culture tubes 7 of the invention greatly provides the efficacy of comparative tests in the development process.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A high-efficiency cell culture tube comprises a culture tube cultivation frame (8), and is characterized in that a plurality of culture tubes (7) which are uniformly inserted into the top wall of the culture tube cultivation frame (8) are arranged on the culture tube cultivation frame (8);
every culture tube (7) is including culture tube body (5), wherein is equipped with the fixed cover of culture tube (3) on culture tube body (5) circumference wall top, is equipped with culture tube lid (2) at the fixed cover of culture tube (3) overcoat, is equipped with culture tube ventilated membrane (4) at culture tube body (5) inside wall, supports through supporting network (6) between the bottom of culture tube ventilated membrane (4) and culture tube body (5), is equipped with filter paper (1) on culture tube lid (2) roof.
2. The high potency cell culture tube according to claim 1, wherein: the culture tube fixing sleeve (3) is connected with the culture tube cover (2) through threads.
3. The high potency cell culture tube according to claim 2, wherein: the threaded connection adopts a double-helix thread structure.
4. The high potency cell culture tube according to claim 1, wherein: a plurality of limiting bulges are arranged between the bottom of the inner side of the culture tube body (5) and the supporting net (6).
5. The high potency cell culture tube according to claim 1, wherein: two limiting rings which are arranged up and down are arranged between the culture tube breathable film (4) and the inner side wall of the culture tube body (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110364783.1A CN113061521A (en) | 2021-04-06 | 2021-04-06 | High-effect cell culture tube |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110364783.1A CN113061521A (en) | 2021-04-06 | 2021-04-06 | High-effect cell culture tube |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113061521A true CN113061521A (en) | 2021-07-02 |
Family
ID=76565772
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110364783.1A Pending CN113061521A (en) | 2021-04-06 | 2021-04-06 | High-effect cell culture tube |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113061521A (en) |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002204690A (en) * | 2001-01-09 | 2002-07-23 | Sumitomo Bakelite Co Ltd | Cell-adhered cell incubator, method for producing the same and method for using the same |
| US20090148941A1 (en) * | 2007-07-30 | 2009-06-11 | Peter Florez | Disposable mini-bioreactor device and method |
| US20110287529A1 (en) * | 2008-09-26 | 2011-11-24 | Corestem Co., Ltd. | Syringe-Shaped Culture Tube and Cell Culture Apparatus Using Same |
| CN204803326U (en) * | 2015-06-30 | 2015-11-25 | 广州洁特生物过滤股份有限公司 | Cell culture changes bottle |
| CN207362219U (en) * | 2017-10-18 | 2018-05-15 | 上海柏根生物科技有限公司 | A kind of suspending cell culture bottle |
| CN109609379A (en) * | 2019-01-21 | 2019-04-12 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel bilateral vapor-permeable type culture bottle |
| CN109762740A (en) * | 2019-02-22 | 2019-05-17 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel hand-held bilateral vapor-permeable type culture bottle |
| CN208933384U (en) * | 2018-09-27 | 2019-06-04 | 潍坊市康华生物技术有限公司 | A kind of culture tube |
| CN209352928U (en) * | 2019-04-03 | 2019-09-06 | 河北森朗生物科技有限公司 | A kind of high density suspension cell culture oxygen increase shaking bottle |
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
| CN212581931U (en) * | 2020-04-17 | 2021-02-23 | 上海基科生物化学有限公司 | Cell culture fixing device for biological research and development |
-
2021
- 2021-04-06 CN CN202110364783.1A patent/CN113061521A/en active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2002204690A (en) * | 2001-01-09 | 2002-07-23 | Sumitomo Bakelite Co Ltd | Cell-adhered cell incubator, method for producing the same and method for using the same |
| US20090148941A1 (en) * | 2007-07-30 | 2009-06-11 | Peter Florez | Disposable mini-bioreactor device and method |
| US20110287529A1 (en) * | 2008-09-26 | 2011-11-24 | Corestem Co., Ltd. | Syringe-Shaped Culture Tube and Cell Culture Apparatus Using Same |
| CN204803326U (en) * | 2015-06-30 | 2015-11-25 | 广州洁特生物过滤股份有限公司 | Cell culture changes bottle |
| CN207362219U (en) * | 2017-10-18 | 2018-05-15 | 上海柏根生物科技有限公司 | A kind of suspending cell culture bottle |
| CN208933384U (en) * | 2018-09-27 | 2019-06-04 | 潍坊市康华生物技术有限公司 | A kind of culture tube |
| CN109609379A (en) * | 2019-01-21 | 2019-04-12 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel bilateral vapor-permeable type culture bottle |
| CN109762740A (en) * | 2019-02-22 | 2019-05-17 | 英诺维尔智能科技（苏州）有限公司 | A kind of novel hand-held bilateral vapor-permeable type culture bottle |
| CN209352928U (en) * | 2019-04-03 | 2019-09-06 | 河北森朗生物科技有限公司 | A kind of high density suspension cell culture oxygen increase shaking bottle |
| CN212581931U (en) * | 2020-04-17 | 2021-02-23 | 上海基科生物化学有限公司 | Cell culture fixing device for biological research and development |
| CN111548938A (en) * | 2020-06-09 | 2020-08-18 | 英诺维尔智能科技（苏州）有限公司 | Novel high-performance culture bottle applicable to suspension and adherent cell mixed culture |
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Application publication date: 20210702 |
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