CN204803322U

CN204803322U - Novel cell culture changes bottle

Info

Publication number: CN204803322U
Application number: CN201520465357.7U
Authority: CN
Inventors: 方想元; 李慧伦; 袁晔; 袁建华
Original assignee: Guangzhou Jie Te Biofiltration Limited-Liability Co
Current assignee: Guangzhou Jie Te Biofiltration Limited-Liability Co
Priority date: 2015-06-30
Filing date: 2015-06-30
Publication date: 2015-11-25
Anticipated expiration: 2025-06-30

Abstract

Novel cell culture changes bottle, including having the open -ended bottle, bottleneck and bottle lid with the open end fixed connection of bottle, the bottle lid is including the circumferential lateral wall of lower surface that is used for sealing bottle open -ended roof and is located the roof, be provided with the internal thread on the lateral wall, be provided with on the bottleneck with internal thread assorted external screw thread and first arch, the lateral wall epirelief is equipped with the recess that supplies first protruding joint when rotatory bottle lid, this first protruding joint in the recess time internal thread form an air guide clearance that supplies gas introduction with matching the external screw. The utility model discloses a novel cell culture changes bottle, bottle lid and bottleneck screw -thread fit for the bottle is inside can to be sealed up, when the bottle need be breathed freely, conveniently regulates and control the air penetrability in the bottle, and the cell culture thing can to some extent not revealed in the bottle simultaneously, and can not cross contamination, and the cell culture effect is better.

Description

Novel cell cultivates rolling bottle

Technical field

The utility model relates to cell co-culture tool technical field, is specifically related to a kind of novel cell and cultivates rolling bottle.

Background technology

When carrying out animal cells in vitro and cultivating, need to provide certain oxygen and carbonic acid gas to maintain the pH value of culture systems in animal cells in vitro cultivation to culture systems, make cell proper splitting, propagation.Thus Tissue Culture Flask is extremely important as the conventional supply of its ventilation property of cell culture apparatus to oxygen or carbonic acid gas.In existing Tissue Culture Flask, mainly comprise body, bottleneck and bottle cap, but ubiquity problem: most Tissue Culture Flask does not have ventilating structure, elasticity mainly by controlling bottle cap controls the circulation of oxygen and carbonic acid gas, to ensure the acid base equilibrium of nutrient solution in culturing bottle, this mode, be difficult to the elasticity getting hold of bottle cap, generally after screwing, half-turn is unscrewed again for degree during open culture, but often air permeable effect is unstable, Medium's PH Value in cell bottle and osmotic pressure cannot maintain perfect condition, if the pine excessively more seriously twisted causes nutrient solution to spill or crossed contamination, and during airtight cultivation, lid tension makes gas exchange, accelerate to break acid base equilibrium in culture system, meanwhile, cause screw socket stripped thread, destroy the stopping property of culturing bottle.

Utility model content

For the deficiencies in the prior art, the purpose of this utility model is to provide a kind of novel cell to cultivate rolling bottle, can better control the air penetrability in bottle, cell cultures better effects if.

For achieving the above object, the utility model adopts following technical scheme:

Novel cell cultivates rolling bottle, comprise the bottle with opening, the bottleneck be fixedly connected with the opening end of bottle and bottle cap, bottle cap comprise for closed bottle opening roof and be positioned at the sidewall of lower surface periphery of roof, sidewall is provided with internal thread, bottleneck is provided with the outside screw and the first projection that match with internal thread, sidewall is convexly equipped with the groove for the first protruding clamping when rotary bottle cover, when this first projection is connected in groove, internal thread coordinates the air-guiding gap that formation one supplied gas imports with outside screw.

Preferably, side wall inner surfaces is convexly equipped with multiple second projection, groove is surrounded by adjacent two the second projections.

Preferably, the quantity of the first projection is equal with the quantity of groove.

Preferably, the first projection and groove are provided with three.

The beneficial effects of the utility model are:

Compared to prior art, novel cell of the present utility model cultivates rolling bottle, bottle cap coordinates with bottleneck thread, bottle inside can be sealed, when bottle needs ventilative, the first projection on bottleneck can be made to be embedded in the groove on sidewall by rotary bottle cover, gas internal thread of postponing is enable to coordinate the air-guiding gap formed to import in bottle with outside screw, air penetrability in conveniently regulating and controlling bottle, bottle inner cell culture can not revealed to some extent simultaneously, and can not crossed contamination, cell cultures better effects if.

Accompanying drawing explanation

Fig. 1 is the structural representation of bottle of the present utility model;

Fig. 2 is the structural representation of bottle cap of the present utility model.

Wherein, 10, bottle; 20, bottleneck; 21, outside screw; 22, first is protruding; 30, bottle cap; 31, internal thread; 32, groove; 33, second is protruding.

Embodiment

Below, by reference to the accompanying drawings and embodiment, the utility model is described further:

Novel cell as shown in Figure 1, 2 cultivates rolling bottle, and comprise and have bottle 10, bottleneck 20 and bottle cap 30, this bottle 10 one end has opening, conveniently puts into cell culture fluid.Bottleneck 20 is fixedly connected on one end that bottle 10 has opening, at the outside surface of this bottleneck 20, outside screw 21 is set, the internal thread 31 matched with outside screw 21 is set at above-mentioned bottle cap 30 internal surface accordingly, make bottle cap 30 be threaded with the opening of sealed bottle body 10 with bottleneck 20, so contribute to the Growth of Cells in bottle 10.

Comprise roof roof for closed bottle 10 opening and sidewall at above-mentioned bottle cap 30, this sidewall is positioned at the lower surface periphery of roof, is specifically arranged on the internal surface of this sidewall at above-mentioned internal thread 31.In addition, sidewall is also provided with groove 32, first projection 22 on above-mentioned bottleneck 20 accordingly, when needs are breathed freely to bottle 10, rotary bottle cover 30, this first projection 22 is made to be connected in groove 32, now, the outside screw 21 on bottleneck 20 is positioned at and the internal thread 31 be positioned on sidewall can coordinate formation one air-guiding gap, to control the circulation of gas (as oxygen and carbonic acid gas etc.) in bottle 10, make the Growth of Cells environment in bottle 10 better, handled easily.

Preferably, in the present embodiment, multiple second projection 33 can be convexly equipped with on the sidewall of above-mentioned bottle cap 30, concrete above-mentioned groove 32 can be surrounded by two second adjacent projections 33, more smooth and easy so that bottle cap 30 is locked when bottle cap 30 can be enable to rotate forward on the other hand, and the raising air penetrability of further bottle 10, on the other hand can conveniently to the processing of bottle cap 30.

Preferably, the quantity of above-mentioned first projection 22 is equal with the quantity of groove 32, and the first projection 22 is connected in corresponding groove 32, makes the Air permenbility in bottle 10 more even; Further, above-mentioned first projection 22 and groove 32 are provided with three in the present embodiment, contribute to the air penetrability in stable bottle 10 on the one hand, also can improve the stability of bottle cap 30 and bottleneck 20 syndeton on the other hand.

Novel cell of the present utility model cultivates rolling bottle, bottle cap 30 and bottleneck 20 thread fit, bottle 10 inside can be sealed, when bottle 10 needs ventilative, the first projection 22 on bottleneck 20 can be made to be embedded in the groove 32 on sidewall by rotary bottle cover 30, gas internal thread 31 of postponing is enable to coordinate the air-guiding gap formed to import in bottle 10 with outside screw 21, air penetrability in conveniently regulating and controlling bottle 10, bottle inner cell culture can not revealed to some extent simultaneously, and can not crossed contamination, cell cultures better effects if.

To one skilled in the art, according to technical scheme described above and design, other various corresponding change and deformation can be made, and all these change and deformation all should belong within the protection domain of the utility model claim.

Claims

1. novel cell cultivates rolling bottle, comprise the bottle with opening, the bottleneck be fixedly connected with the opening end of bottle and bottle cap, it is characterized in that, bottle cap comprise for closed bottle opening roof and be positioned at the sidewall of lower surface periphery of roof, sidewall is provided with internal thread, bottleneck is provided with the outside screw and the first projection that match with internal thread, sidewall is convexly equipped with the groove for the first protruding clamping when rotary bottle cover, when this first projection is connected in groove, internal thread coordinates the air-guiding gap that formation one supplied gas imports with outside screw.

2. novel cell as claimed in claim 1 cultivates rolling bottle, it is characterized in that, side wall inner surfaces is convexly equipped with multiple second projection, groove is surrounded by adjacent two the second projections.

3. novel cell as claimed in claim 1 cultivates rolling bottle, and it is characterized in that, the quantity of the first projection is equal with the quantity of groove.

4. novel cell as claimed in claim 1 cultivates rolling bottle, it is characterized in that, the first projection and groove are provided with three.