CN206872850U - A kind of animal cell culture device - Google Patents
A kind of animal cell culture device Download PDFInfo
- Publication number
- CN206872850U CN206872850U CN201720510727.3U CN201720510727U CN206872850U CN 206872850 U CN206872850 U CN 206872850U CN 201720510727 U CN201720510727 U CN 201720510727U CN 206872850 U CN206872850 U CN 206872850U
- Authority
- CN
- China
- Prior art keywords
- cell culture
- ware
- lid
- discharge nozzle
- adjuster
- Prior art date
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- Expired - Fee Related
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Abstract
The utility model discloses a kind of animal cell culture device, including ware body and ware lid, the ware body includes bottom, annular sidewall, annular bottom wall, cell culture chamber is formed between the upper surface of the bottom and the inner surface of annular sidewall；The ware lid includes top, annular side portions, and whole ware body is located in constant temperature and humidity perseverance oxygen adjuster；The constant temperature and humidity perseverance oxygen adjuster includes refrigerating and heating combined equipment, humidification device and fresh air-changing device.The cell culture chamber has arc bottom wall, and the bottom centre position of the arc bottom wall is provided with discharge nozzle, and discharge nozzle communicates with cell culture chamber interior, and adjuster is provided with the discharge nozzle, and the adjuster is used for the opening degree for adjusting discharge nozzle.By being provided with arc bottom wall and discharge nozzle, it is easy to discharge the zooblast after culture.Covered in the ware and pipette is installed, and pipette communicates with cell culture chamber interior, and lid is installed on pipette, cap handle is installed on the lid.
Description
Technical field
It the utility model is related to animal cell culture technology field, more particularly to a kind of animal cell culture device.
Background technology
Incubator is a kind of labware for cell culture, it is general mainly by a flat circle plate-like bottom and
One lid composition.Incubator is essentially divided into two classes according to material, and one kind is plastics, another kind for glass, glass can be with
Adhere-wall culture for vegetable material, microculture and zooblast may also be used.Plastics are probably polythene material
, once property and it is nonexpondable, be adapted to laboratory inoculation, line, the operation of separation of bacterial, can be used for vegetable material
Culture.
Zooblast in incubation, it is necessary to gnotobasis, and either internal cell or cell in vitro, it is necessary to
Survived under stationary temperature, general optimum growth temperature be 37 DEG C, temperature change is not to be exceeded ± 0.5 DEG C, this mainly by
What the optimum temperature needed for enzyme and protein was determined.When temperature is too low, cell growth does not grow even slowly, and temperature is too high
When, cell death can be caused, when temperature surpasses 2 DEG C, be then unfavorable for cells survival, temperature reaches more than 40 DEG C cells will be dead quickly
Die.Current animal cell culture device simply can carry out temperature adjustment, it is impossible to which the humidity required for cell culture, oxygen are contained
Amount, air purity are adjusted.Existing cell culture apparatus can not be effective by cell in time after culture is completed simultaneously
Discharge.
Utility model content
The utility model is in order to overcome deficiency of the prior art, there is provided a kind of animal cell culture device.
The utility model is to be achieved through the following technical solutions：
A kind of animal cell culture device, including for placing the ware body of culture medium and the ware made of transparent high temperature resistant material
Lid, the ware body include bottom, the annular sidewall of setting, the following table from the bottom are upwardly extended from the upper surface of the bottom
The annular bottom wall of setting is extended outwardly, cell culture is formed between the upper surface of the bottom and the inner surface of annular sidewall
Room；The ware lid extends outwardly the annular side portions of setting including top, the following table from the top, at the top of the ware lid
On be provided with rotating handle.Whole ware body is located in constant temperature and humidity perseverance oxygen adjuster；The constant temperature and humidity perseverance oxygen adjuster includes system
Cold heating combined equipment, humidification device and fresh air-changing device.The cell culture chamber has arc bottom wall, in the arc bottom wall
Bottom centre position is provided with discharge nozzle, and discharge nozzle communicates with cell culture chamber interior, and is provided with the discharge nozzle
Adjuster, the adjuster are used for the opening degree for adjusting discharge nozzle.By being provided with arc bottom wall and discharge nozzle, it is easy to cultivate
Zooblast afterwards discharges.Covered in the ware and pipette is installed, and pipette communicates with cell culture chamber interior, and
Lid is installed on pipette, cap handle is installed on the lid.Seal groove is machined with the bottom outer surface of the ware body
A, sealing ring A is installed in the seal groove A, seal groove B is machined with the annular sidewall outer surface of the ware body, described
Sealing ring B is installed in seal groove B.
Compared with prior art, the beneficial effects of the utility model are：The utility model is by being provided with constant temperature and humidity
Permanent oxygen adjuster, can realize and the air themperature in animal cell culture environment, humidity, oxygen content are controlled, so as to give
Animal cell culture builds good environment；By being provided with arc bottom wall and discharge nozzle, it is easy to the zooblast after culture
Discharge；By being provided with the culture base table of pipette, oese or suction pipette head by pipette into cell culture chamber
Face is inoculated with or sample-adding；By being provided with sealing ring A and sealing ring B, it is possible to increase the sealing property between ware body and ware lid, can
Prevent in transportation, cross pollution occurs with the microorganism outside culture dish in culture dish.
Brief description of the drawings
Fig. 1 is structural representation of the present utility model.
Embodiment
In order that the purpose of this utility model, technical scheme and advantage are more clearly understood, below in conjunction with accompanying drawing and implementation
Example, the utility model is further elaborated.It should be appreciated that specific embodiment described herein is only explaining
The utility model, it is not used to limit the utility model.
Referring to Fig. 1, a kind of described animal cell culture device, including for placing the ware body 1 of culture medium and by transparent
Ware lid 2 made of exotic material, the ware body 1 include bottom 11, upwardly extend setting from the upper surface of the bottom 11
Annular sidewall 12, the following table from the bottom 11 extend outwardly the annular bottom wall 13 of setting, the upper surface of the bottom 11 with
Cell culture chamber 3 is formed between the inner surface of annular sidewall 12；The ware lid 2 includes top 21, the following table from the top 21
The annular side portions 22 of setting are extended outwardly, rotating handle 23 is provided with the top 21 of the ware lid 2.
Whole ware body 1 is located in constant temperature and humidity perseverance oxygen adjuster 4；The constant temperature and humidity perseverance oxygen adjuster 4 includes refrigerated medium
Thermal, humidification device and fresh air-changing device.What deserves to be explained is the constant temperature and humidity perseverance oxygen adjuster 4 in the utility model
Operation principle is referred to the utility model patent of Chinese Application No. 201120293167.3, and patent name is《Constant temperature and humidity
Permanent oxygen air regulator》.
The cell culture chamber 3 has arc bottom wall 31, is provided with out in the bottom centre position of the arc bottom wall 31
Expects pipe 32, and discharge nozzle 32 is inside cell culture chamber 3 with communicating, and adjuster 33 is installed on the discharge nozzle 32, it is described
Adjuster 33 is used for the opening degree for adjusting discharge nozzle 32.By being provided with arc bottom wall 31 and discharge nozzle 32, it is easy to after culture
Zooblast discharge.
Pipette 5 is installed on the ware lid 2, and pipette 5 is inside cell culture chamber 3 with communicating, and in pipette 5
On lid 51 is installed, cap handle 52 is installed on the lid 51.Oese or suction pipette head are by pipette 5 to cell
Media surface inoculation or sample-adding in culturing room 3.
Seal groove A is machined with the outer surface of bottom 11 of the ware body 1, sealing ring is installed in the seal groove A
A14, seal groove B is machined with the outer surface of annular sidewall 12 of the ware body 1, sealing ring is installed in the seal groove B
B15.By being provided with sealing ring A14 and sealing ring B15, it is possible to increase the sealing property between ware body 1 and ware lid 2, Neng Goufang
Only in transportation, cross pollution occurs with the microorganism outside culture dish in culture dish.
Preferred embodiment of the present utility model is above are only, it is all in this practicality not to limit the utility model
All any modification, equivalent and improvement made within new spirit and principle etc., should be included in guarantor of the present utility model
Within the scope of shield.
Claims (4)
1. a kind of animal cell culture device, including for placing the ware body (1) of culture medium and made of transparent high temperature resistant material
Ware lid (2), the ware body (1) include bottom (11), the annular sidewall of setting are upwardly extended from the upper surface of the bottom (11)
(12), the following table from the bottom (11) extends outwardly the annular bottom wall (13) of setting, the upper surface of the bottom (11) with
Cell culture chamber (3) is formed between the inner surface of annular sidewall (12)；The ware lid (2) includes top (21), from the top
(21) following table extends outwardly the annular side portions (22) of setting；It is characterized in that：Whole ware body (1) is located at constant temperature and humidity perseverance oxygen
In adjuster (4)；The cell culture chamber (3) has arc bottom wall (31), in the bottom interposition of the arc bottom wall (31)
Install and be equipped with discharge nozzle (32), and discharge nozzle (32) communicates with cell culture chamber (3) inside, and pacify on the discharge nozzle (32)
Equipped with adjuster (33), the adjuster (33) is used for the opening degree for adjusting discharge nozzle (32)；It is provided with the ware lid (2)
Pipette (5), and pipette (5) communicates with cell culture chamber (3) inside, and lid (51) is installed on pipette (5),
Cap handle (52) is installed on the lid (51).
A kind of 2. animal cell culture device according to claim 1, it is characterised in that：In the bottom of the ware body (1)
(11) outer surface is machined with seal groove A, and sealing ring A (14) is provided with the seal groove A, in the annular of the ware body (1)
Side wall (12) outer surface is machined with seal groove B, and sealing ring B (15) is provided with the seal groove B.
A kind of 3. animal cell culture device according to claim 2, it is characterised in that：At the top of the ware lid (2)
(21) rotating handle (23) is provided with.
A kind of 4. animal cell culture device according to claim 1, it is characterised in that：The constant temperature and humidity perseverance oxygen adjuster
(4) refrigerating and heating combined equipment, humidification device and fresh air-changing device are included.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720510727.3U CN206872850U (en) | 2017-05-09 | 2017-05-09 | A kind of animal cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201720510727.3U CN206872850U (en) | 2017-05-09 | 2017-05-09 | A kind of animal cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN206872850U true CN206872850U (en) | 2018-01-12 |
Family
ID=61338906
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201720510727.3U Expired - Fee Related CN206872850U (en) | 2017-05-09 | 2017-05-09 | A kind of animal cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN206872850U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111032851A (en) * | 2018-07-13 | 2020-04-17 | 康宁股份有限公司 | Microvessel having side wall comprising liquid medium transfer surface |
-
2017
- 2017-05-09 CN CN201720510727.3U patent/CN206872850U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111032851A (en) * | 2018-07-13 | 2020-04-17 | 康宁股份有限公司 | Microvessel having side wall comprising liquid medium transfer surface |
| CN111032851B (en) * | 2018-07-13 | 2024-03-29 | 康宁股份有限公司 | Microcavity dish having a sidewall with a liquid medium transfer surface |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180112 Termination date: 20180509 |