CN201605269U - Breathing window type solid state fermentation system - Google Patents

Breathing window type solid state fermentation system Download PDF

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Publication number
CN201605269U
CN201605269U CN 200920181811 CN200920181811U CN201605269U CN 201605269 U CN201605269 U CN 201605269U CN 200920181811 CN200920181811 CN 200920181811 CN 200920181811 U CN200920181811 U CN 200920181811U CN 201605269 U CN201605269 U CN 201605269U
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window
ventilation
solid state
state fermentation
circulating
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李星洪
王多祥
周维金
彭立娥
吴永慧
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XIAMEN TOBEFAR TECHNOLOGY Co Ltd
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XIAMEN TOBEFAR TECHNOLOGY Co Ltd
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    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/16Solid state fermenters, e.g. for koji production
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/30Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration
    • C12M41/34Means for regulation, monitoring, measurement or control, e.g. flow regulation of concentration of gas

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Abstract

The utility model relates to a breathing window type solid state fermentation system, which relates to a solid state fermentation system. The breathing window type solid state fermentation system, which is designed according to the thermodynamic principle, comprises a breathing window type solid state fermentation tank and a solid state fermentation control room, wherein an air temperature and humidity regulating and controlling device and a sterile air and cooling circulating water system are arranged in the solid state fermentation control room; and as the fermentation tank is arranged in the solid state fermentation control room, the temperature and the humidity in solid state fermentation tank can be effectively controlled by regulating and controlling the temperature and the humidity in the solid state fermentation control room. The fermentation tank adopts a rectangular tank body made of thin metal sheets, wherein breathing windows are formed on the front door leaf and the rear wall, a forced draught oxygen supply heat conduction circulating system composed of a vortex air pump driven by magnetic coupling, a circulating air duct, a circulating air duct water cooler, a gas distributing room, a parallel air draft tube, a drainage tube and a gas collecting chamber are arranged in the fermentation tank in sequence, and mobile shallow disk bracket capable of placing multi-layers of solid state fermentation shallow disks and shallow disks are arranged in the tank; the utility model can be used for the pure cultivation of microorganisms, is avoided from the three wastes and is applicable in the industrial production of bio-enzyme preparations.

Description

Window for ventilation formula solid state fermentation system
Technical field
The utility model relates to a kind of solid state fermentation equipment, especially relates to a kind of Window for ventilation formula solid state fermentation system.
Background technology
Since 1945 realize penicillin liquid submerged fermentation large-scale industrial production, because liquid submerged fermentation can carry out pure culture, the condition of microbial fermentation can be carried out automatization control, the manufacturing technology of liquid state fermentation equipment is gradually improved, so in the suitability for industrialized production of microbial fermentation product, dominate still so far.But the liquid submerged fermentation facility investment is big, production cost is high, produce a large amount of " three wastes ", contaminate environment in the production process.
Solid state fermentation has a long history in China, compare with liquid submerged fermentation, the theoretical question of many solid state fermentations, wait further research, fermentation technique is relative with fermentation equipment backward, but it has the advantage that liquid submerged fermentation does not possess, and for example equipment is simple relatively, less investment, the output capacity height of product, several times that generally can reach liquid submerged fermentation to ten times, essentially no " three wastes " discharging, non-environmental-pollution, save the energy, can utilize the waste of agriculture production and agricultural byproducts processing, and natural various fiber forages etc. are raw material, raw material sources are wide, and price is very cheap.Solid state fermentation can be widely used in the processing of food, beverage, alcohol, biological system medicine, biological pesticide, biological enzyme formulation, organic acid, single cell protein bletilla solid-state castoff and environment protection etc.
The root problem that solid state fermentation exists technically is the living contaminants of fermenting process and is difficult to carry out large-scale industrial production.The spontaneous fermentation mode that the existing fermentation mode of China remains traditional is main, comprises koji tray, Quchi, box thick-layer ventilating fermentation, thick-layer ventilation pond formula, the continuous shallow tray fermentation of tunnel furnace etc.The domestic in recent years solid-state fermenter of developing several sealings is the static box or cartridge type fermentor tank that static state is box or interband is had a rest and stirred mostly, or tray horizontal cylinder fermentor tank etc., be a a progressive step than spontaneous fermentation mode, but still have following problem:
1) equipment heaviness, inconvenience is moved, and what have can not be used for industrial production at all, as horizontal stirring cylindrical shell fermentor tank.
2) facility investment height, the fermentor tank utilization index is low, can not realize large-scale industrial production.
3) can not avoid polluting in seeded process, it is not thorough to sterilize, and can not realize purebred cultivation.
4) a jar interior temperature humidity regulate and control method falls behind, and as temperature chuck commonly used or the control of tubulation water temperature, it is big to take useful space, and humidity causes pollution easily with direct water injection or humidifier spraying in the jar.
5) patent that has charges into sterile air to 1.5~3.5kg/cm in jar when shallow tray fermentation 2, keep 1~5min after, pressure release to 0.1~0.5kg/cm 2, keep after 10~60s, circulate repeatedly above-mentioned pressurising and pressure leak process, energy consumption is too big, and can cause a large amount of volatilizations of fermentation materials moisture.
Summary of the invention
The purpose of this utility model provides a kind ofly can carry out purebred cultivation, can modularization enlarge production again, can be automatically effectively controlled temperature, humidity and oxygen content, carry out the transmission of heat and material, equipment cost is low, removable, wide adaptability can be used for the Window for ventilation formula solid state fermentation system that industrialization such as biological enzyme formulation is produced.
The utility model is provided with between Window for ventilation formula solid-state fermenter, solid state fermentation control and moistureproof drive-motor, in Window for ventilation formula solid-state fermenter is located between solid state fermentation control, sets up the air themperature regulation device that is useful between the control of regulation and control solid state fermentation between solid state fermentation control, be used to regulate and control atmospheric moisture regulation device, sterile air system and cooling circulating water system between solid state fermentation control; Window for ventilation formula solid-state fermenter is the cuboid tank body of a sheet metal structure, and Window for ventilation formula solid-state fermenter is provided with at least 1 button fly front fan, is respectively equipped with at least 2 Window for ventilation on the rear wall of button fly front fan and Window for ventilation formula solid-state fermenter; Window for ventilation formula solid-state fermenter is provided with vortex air pump, circulating ventilation pipe and the circulating ventilation pipe watercooler of magnetic force couple drive outward, the circulating ventilation pipe is provided with oxygen on-line monitoring interface, sterile air interface, temp probe interface and moisture probe interface, circulating ventilation pipe watercooler is provided with water coolant water inlet interface and water coolant water outlet interface, and 4 pin of Window for ventilation formula solid state fermentation pot bottom are provided with the roller of bearing; Be provided with gas distribution chamber, Parallel airflow thrust-augmenting nozzle, drainage tube, gas in the Window for ventilation formula solid-state fermenter and compile chamber and tray frame movably, but tray frame horizontal positioned multilayer from top to bottom fills the tray of solid medium, and moistureproof drive-motor is connected with magnetic force coupled vortex air pump fast-assembling by the convex-concave shaft joint.
At least 2 little can be divided between described solid state fermentation control, at least 2 Window for ventilation formula solid-state fermenters can be placed for each little.
The button fly front fan of described Window for ventilation formula solid-state fermenter, it can be the single open-door fan, the double door fan or the fan that opens the door more, the doorframe of button fly front fan is provided with seal groove, and resistant to elevated temperatures sealing-ring is housed, the button fly front fan pass is crossed hinge and is installed on the doorframe, when door is closed, realizes tightening seal by the resistant to elevated temperatures sealing-ring that upwards turns over screw, door lock down and be installed on the doorframe.
The Window for ventilation of described Window for ventilation formula solid-state fermenter can be the rectangle of band fillet, the Window for ventilation of circle or other shape, each Window for ventilation can be equipped with at least 1 respiratory membrane, described respiratory membrane is preferably 0.3 μ m micro particle filtering efficient up to 99.97% respiratory membrane, respiratory membrane is resistant to elevated temperatures organic membrane or mineral membrane, organic membrane is polypropylene screen or poly tetrafluoroethylene etc., mineral membrane is a ceramic fibre film etc., respiratory membrane can be the hybrid films that single or multiple lift is formed, respiratory membrane can allow air and airborne water molecules freely diffuse through, airborne assorted bacterium and spore thereof can not pass through, and respiratory membrane is by the stainless steel pressure ring, resistant to elevated temperatures sealing-ring, the screw tightening seal is installed on the window of Window for ventilation.
Described Window for ventilation formula solid-state fermenter is provided with a forced ventilation oxygen supply heat-conducting circulating system of being made up of vortex air pump, circulating ventilation pipe, circulating ventilation pipe watercooler, gas distribution chamber, Parallel airflow thrust-augmenting nozzle, drainage tube and the gas chamber of compiling of magnetic force couple drive successively.Flow through the Parallel airflow bundle that each layer horizontal positioned fills solid medium tray surface in order to make, its fixed air channel is all arranged, Parallel airflow thrust-augmenting nozzle and drainage tube all are that the branch multilayer is horizontal from top to bottom, each layer is established at least 2 Parallel airflow thrust-augmenting nozzles and drainage tube, the outlet of every layer Parallel airflow thrust-augmenting nozzle, just enter the edge of a plurality of trays one side of horizontal placement, drainage tube is in the opposite side of a plurality of trays of horizontal placement, the correspondence position that parallels one by one with the Parallel airflow thrust-augmenting nozzle, the direction that one end of drainage tube blows facing to Parallel airflow, the other end communicates with the gas chamber of compiling.
The ventilation heat-conducting circulating system that described fermentor tank is equipped with is provided with temperature online measurement and control instrument, circulating ventilation pipe watercooler and cooling circulating water system, the measurement and control of temperature probe of temperature online measurement and control instrument joins with the measurement and control of temperature probe interface, the water outlet of cooling circulating water system and water-in join by the water-in and the water outlet of magnetic valve and circulating ventilation pipe watercooler respectively, and the magnetic valve of water-in and water outlet is controlled by the temperature online measurement and control instrument.
The ventilation oxygen supply recycle system that described fermentor tank is equipped with is provided with oxygen on-line monitoring device, sterile air system, sterile air interface and oxygen on-line monitoring interface; The observing and controlling probe of oxygen on-line monitoring device and oxygen on-line monitoring interface join, and the outlet of sterile air system is connected by magnetic valve with the sterile air interface, and the switch of magnetic valve is controlled by oxygen on-line monitoring device; When oxygen-consumption was too high in the fermentor tank, the magnetic valve of in time opening the sterile air interface automatically charged into fresh sterile air, carries out the forced ventilation circulation simultaneously the oxygen concentration in the fermentor tank is elevated near normal range.
Described cuboid tank body can be the cuboid tank body of metal plate structure, the inner frame of preferably thin stainless steel plate shell of described cuboid tank body and stainless steel angle steel system.
Described sealing-ring can be resistant to elevated temperatures silicone rubber O-ring or teflin ring etc.
The Window for ventilation formula solid state fermentation system that the utility model provides, according to thermodynamics about thermal conduction and the concentration diffusion principle design of material, it comprises between Window for ventilation formula solid-state fermenter and solid state fermentation control, Window for ventilation formula solid-state fermenter has been set up air themperature regulation device, atmospheric moisture regulation device, sterile air system and cooling circulating water system between solid state fermentation control in being between solid state fermentation control.Temperature and humidity in the Window for ventilation solid-state fermenter is controlled by the temperature and humidity between the control of regulation and control solid state fermentation.Window for ventilation formula solid-state fermenter is the tank body of the cuboid of a sheet metal structure, and the front of tank body is the button fly front fan, on button fly front fan and the rear wall at least 2 Window for ventilation is arranged respectively.Window for ventilation formula solid-state fermenter is provided with vortex air pump, circulating ventilation pipe, the circulating ventilation pipe watercooler of magnetic force couple drive outward, and the circulating ventilation pipe is provided with oxygen on-line monitoring interface, sterile air interface and temperature, moisture probe interface.Circulating ventilation pipe watercooler is provided with water coolant water inlet interface and water outlet interface.Four pin of Window for ventilation formula solid-state fermenter bottom are equipped with the roller of bearing.There are gas distribution chamber, Parallel airflow thrust-augmenting nozzle, drainage tube, gas to compile chamber, tray frame movably in the Window for ventilation formula solid-state fermenter, but tray frame horizontal positioned multilayer from top to bottom fills the tray of solid medium, and the tray frame can freely load and unload with handling machinery together with the tray that is contained on the tray frame.Moistureproof drive-motor is connected with magnetic force coupled vortex air pump fast-assembling by the convex-concave shaft joint.
The tank body feature of above Window for ventilation formula solid-state fermenter and the feature of Window for ventilation can realize one of thought of the present utility model, be the temperature and humidity in the Window for ventilation solid-state fermenter, the temperature and humidity between controlling by the regulation and control solid state fermentation is controlled and living contaminants can not be taken place.Concrete principle is: resistant to elevated temperatures organic membrane and mineral membrane that Window for ventilation is equipped with, and it can allow air and airborne water molecules freely diffuse through, but assorted bacterium and assorted bacterium spore can not pass through.Tank body in addition ferments, particularly rectangular parallelepiped approaches the fermentation tank body of stainless steel plate shell structure, heat transfer area is big, press thermodynamics about thermal conduction and the concentration diffusion principle of material, utilize such Window for ventilation and tank body material structure can realize in the jar of Window for ventilation formula solid-state fermenter and a jar outer substance transfer and a transmission ofenergy, until reaching balance.Here the substance transfer of indication specifically refers to, airborne water molecules and oxygen molecule see through film to humidity and the lower jar internal diffusion transfer of oxygen concentration between solid state fermentation control, transmission ofenergy specifically refers to a jar heat interior and that jar is outer and is transmitted to the low one side of temperature by the high one side of temperature, therefore, as long as the temperature and humidity between the control of control solid state fermentation can realize that just test-results shows to the aseptic control of humiture in the Window for ventilation formula solid-state fermenter, easy to use, pratical and feasible.
Because Window for ventilation formula solid-state fermenter has one successively by the vortex air pump of magnetic force couple drive, the circulating ventilation pipe, circulating ventilation pipe watercooler, the gas distribution chamber, the Parallel airflow thrust-augmenting nozzle, the forced ventilation oxygen supply heat-conducting circulating system that drainage tube and the gas chamber of compiling are formed, flow through the Parallel airflow bundle that each layer horizontal positioned fills solid medium tray surface in order to make, its fixed air channel is all arranged, Parallel airflow thrust-augmenting nozzle and drainage tube all are that the branch multilayer is horizontal from top to bottom, at least 2 on each layer, the outlet of every layer of Parallel airflow thrust-augmenting nozzle, just enter the edge of a plurality of trays one side of horizontal placement, an opposite side that is in a plurality of trays of horizontal placement of drainage tube, with the correspondence position that the Parallel airflow thrust-augmenting nozzle parallels one by one, the other end level of drainage tube is communicated with the gas chamber of compiling.Therefore this forced ventilation recycle system has following characteristics: the vortex air pump of magnetic force couple drive promotes recirculating air and moves towards a direction with bigger blast, the gas distribution chamber that is introduced into solid-state fermenter forms malleation, make recirculating air respectively by the horizontal thrust-augmenting nozzle of branch multilayer from top to bottom, form the Parallel airflow bundle, blow over the surface of fermentation tray, and the biological heat that is diffused into the substratum top layer taken away, meanwhile carrying out gas such as CO in the solid state fermentation matrix granule gap 2With the O in the air flow 2Exchange, at any time replenish the oxygen that consumes in the solid state fermentation matrix granule gap, when the vortex air pump of magnetic force couple drive starts, make with gas that it directly communicates and compile indoor formation negative pressure, the drainage tube that is communicated with the gas chamber of compiling by a level, the Parallel airflow that to blow over the surface of fermentation tray sucks and compiles the chamber, thereby make the Parallel airflow bundle compile the chamber to entering gas by entering, blow over, leave fermentation tray surface, form a fixed air channel, guarantee the successful exchange and the transfer of fermenting process caloic.
The characteristics of above-described forced ventilation oxygen supply heat-conducting circulating system also are: the ventilation heat-conducting circulating system that fermentor tank is equipped with, form by temperature online measurement and control instrument, circulating ventilation pipe watercooler and cooling circulating water system, the measurement and control of temperature probe of temperature online measurement and control instrument joins with the measurement and control of temperature probe interface, and the water outlet of cooling circulating water system and water-in join by the water-in and the water outlet of magnetic valve and circulating ventilation pipe watercooler respectively; The magnetic valve of water inlet and water outlet is controlled by the temperature online measurement and control instrument; The biological heat that produces when the fermentor tank internal cause makes temperature surpass going up in limited time of control rapidly, opens the magnetic valve of water inlet of circulating ventilation pipe watercooler and water outlet automatically, by the forced ventilation circulation temperature in the fermentor tank is reduced to below the controlling valu;
The characteristics of above-described forced ventilation oxygen supply heat-conducting circulating system also are: the ventilation oxygen supply recycle system that fermentor tank is equipped with is made up of oxygen on-line monitoring device, sterile air system, sterile air interface and oxygen on-line monitoring interface; The observing and controlling probe of oxygen on-line monitoring device and oxygen on-line monitoring interface join, and the outlet of sterile air system is connected by magnetic valve with the sterile air interface, and the switch of magnetic valve is controlled by oxygen on-line monitoring device; When oxygen-consumption was too high in the fermentor tank, the magnetic valve of in time opening the sterile air interface charged into fresh sterile air, carries out the forced ventilation circulation simultaneously the oxygen concentration in the fermentor tank is elevated near normal range.
This shows, forced ventilation oxygen supply heat-conducting circulating system of the present utility model, the transfer of heat and the oxygen that efficiently solve in the tray solid ferment process are supplied with, thereby are the formation of normal growth and the product of fermenting process microorganism, have created best growth conditions.
The Window for ventilation formula solid state fermentation system that the utility model provides, about thermal conduction and the concentration diffusion principle design of material, the purpose of this utility model can be realized according to thermodynamics, purebred cultivation can be carried out, can modularization enlarge production again, can be automatically effectively controlled temperature, humidity and oxygen content, carry out the transmission of heat and material, can overcome the general considerations that exists in the present solid state fermentation, equipment cost is low, removable, wide adaptability can be used for industrialization productions such as biological enzyme formulation.
Compared with prior art, there is following outstanding advantage in the Window for ventilation formula solid state fermentation system that the utility model provides:
1) the Window for ventilation formula solid state fermentation system that designs according to thermodynamic principles, because by the temperature and humidity between the control of regulation and control solid state fermentation, realization is to the regulation and control of temperature and humidity in the Window for ventilation formula solid-state fermenter, and the sterilization of the air ambient between control of needn't considering to ferment, therefore have that device is simple, scientific structure, humiture control are convenient accurately, no living contaminants, can improve the solid-state fermenter utilization index, can be completely achieved automatization and control, and have the incomparable advantage of existing solid-state fermenter humiture control general technology.
2) Window for ventilation formula solid state fermentation system comprises that two portions are formed between Window for ventilation formula solid-state fermenter and solid state fermentation control.Between solid state fermentation control is the place that control breathing window formula solid state fermentation ferments, and utilizes this system can realize that the device of shifting block type industrialization enlarges production.
3) provide a kind of modern design and the pump circulation ventilation heat conduction and the online oxygen supervisory system of practical generation Parallel airflow bundle, the surface of every layer of horizontal fermentation tray in fermentor tank, be provided with air channel along fixed-direction, biological transfer of heat control and oxygen supply in the solid-state ferment process of tray have been solved, microbial fermentation optimum growth temp and oxygen environment have been guaranteed, and the useful space in not taking jar, jar the utilization index height.
4) the vortex air pump of the magnetic force couple drive of pump circulation ventilation oxygen supply thermal conductivity use, the blast height, good airproof performance can prevent atmospheric pollution.
5) Window for ventilation formula solid-state fermenter shell is thin stainless steel plate design, and is provided with the roller that 4 tape spools hold, and in light weight, cost is low, and is removable, can not have the sterilization dead angle together with the whole sterilization of fermentor tank, can inoculate at sterilisable chamber, realizes pure culture.
6) the Window for ventilation formula solid state fermentation system that provides of the utility model is applied widely, can be used for the production of multiple microorganism shallow tray fermentation, as biological enzyme formulation, organic acid, and the production of biological medicine, biological pesticide etc.
Description of drawings
Fig. 1 is the structure composition frame chart of the utility model embodiment.
Fig. 2 is the structural representation of the Window for ventilation formula solid-state fermenter of the utility model embodiment.
Fig. 3 is the button fly front of Window for ventilation formula solid-state fermenter of the utility model embodiment and the structural representation of Window for ventilation.
Fig. 4 is the structural representation of the Window for ventilation formula solid-state fermenter rear wall Window for ventilation of the utility model embodiment.
Fig. 5 is the schematic top plan view of the recirculated air watercooler of the utility model embodiment.
Fig. 6 is the distribution schematic diagram of the gas distribution chamber thrust-augmenting nozzle of the utility model embodiment.
Respectively be labeled as among the figure: between Window for ventilation formula solid-state fermenter 1, solid-state fermenter control 2, moistureproof drive-motor 3, convex-concave fast-assembling shaft joint 4, vortex air pump 5, mounting flange 6, mounting flange 7, circulating ventilation pipe 8, oxygen on-line monitoring interface 9, gas compiles chamber 10, drainage tube 11, fermentation tray 12, circulating ventilation pipe watercooler 13, tray frame 14, door leaf 15, doorframe 16, hinge 17, Window for ventilation 18, upwards turn over screw 19 down, water coolant water inlet interface 21, water coolant water outlet interface 22, Parallel airflow thrust-augmenting nozzle 23, gas distribution chamber 24, mounting flange 25, air themperature regulation device 26, atmospheric moisture regulation device 27, sterile air system 28, cooling circulating water system 29, measurement and control of temperature probe interface 35, humidity observing and controlling probe interface 36, the roller 37 that tape spool holds, sterile air interface 38.
Embodiment
Below in conjunction with drawings and Examples the utility model is further described.
The utility model is provided with between the control of Window for ventilation formula solid-state fermenter 1, solid state fermentation 2 and moistureproof drive-motor 3, Window for ventilation formula solid-state fermenter 1 is located between solid state fermentation control in 2, and 2 set up and are useful between the control of regulation and control solid state fermentation 2 air themperature regulation device 26, are used to regulate and control between solid state fermentation control 2 atmospheric moisture regulation device 27, sterile air system 28 and cooling circulating water system 29 between solid state fermentation control; Window for ventilation formula solid-state fermenter 1 is the cuboid tank body of a sheet metal structure, and Window for ventilation formula solid-state fermenter 1 is provided with at least 1 button fly front fan 15, is respectively equipped with at least 2 Window for ventilation 18 on the rear wall of button fly front fan 15 and Window for ventilation formula solid-state fermenter 1; Window for ventilation formula solid-state fermenter 1 outer vortex air pump 5, circulating ventilation pipe 8 and the circulating ventilation pipe watercooler 13 that is provided with the magnetic force couple drive, circulating ventilation pipe 8 is provided with oxygen on-line monitoring interface 9, sterile air interface 38, temp probe interface 35 and moisture probe interface 36, circulating ventilation pipe watercooler 13 is provided with water coolant water inlet interface 21 and water coolant water outlet interface 22, and 4 pin of Window for ventilation formula solid-state fermenter 1 bottom are provided with the roller 37 of bearing; Be provided with gas distribution chamber 24, Parallel airflow thrust-augmenting nozzle 23, drainage tube 11, gas in the Window for ventilation formula solid-state fermenter 1 and compile chamber 10 and tray frame 14 movably, but tray frame 14 horizontal positioned multilayer from top to bottom fills the tray 12 of solid medium, and moistureproof drive-motor 3 is connected with magnetic force coupled vortex air pump 5 fast-assemblings by convex-concave shaft joint 4.
2 can be divided at least 2 little between described solid state fermentation control, can place at least 2 Window for ventilation formula solid-state fermenters 1 for each little.
The button fly front fan 15 of described Window for ventilation formula solid-state fermenter 1, it can be the single open-door fan, the double door fan or the fan that opens the door more, the doorframe 16 of button fly front fan is provided with seal groove, and resistant to elevated temperatures sealing-ring is housed, button fly front fan 15 is installed on the doorframe 16 by hinge 17, when door is closed, realizes tightening seal by the resistant to elevated temperatures sealing-ring that upwards turns over screw 19, door lock 20 down and be installed on the doorframe 16.
The Window for ventilation 18 of described Window for ventilation formula solid-state fermenter 1 can be the rectangle of band fillet, the Window for ventilation of circle or other shape, each Window for ventilation be equipped with at least 1 to 0.3 μ m micro particle filtering efficient up to 99.97% respiratory membrane, respiratory membrane is resistant to elevated temperatures organic membrane or mineral membrane, organic membrane is polypropylene screen or poly tetrafluoroethylene etc., mineral membrane is a ceramic fibre film etc., respiratory membrane can be the hybrid films that single or multiple lift is formed, respiratory membrane can allow air and airborne water molecules freely diffuse through, airborne assorted bacterium and spore thereof can not pass through, and respiratory membrane is by the stainless steel pressure ring, resistant to elevated temperatures sealing-ring, the screw tightening seal is installed on the window of Window for ventilation.
Window for ventilation formula solid-state fermenter 1 is provided with one and compiles the forced ventilation oxygen supply heat-conducting circulating system of forming chamber 10 by vortex air pump 5, circulating ventilation pipe 8, circulating ventilation pipe watercooler 13, gas distribution chamber 24, Parallel airflow thrust-augmenting nozzle 23, drainage tube 11 and the gas of magnetic force couple drive successively; Flow through the Parallel airflow bundle that each layer horizontal positioned fills solid medium tray 12 surfaces in order to make, its fixed air channel is all arranged, Parallel airflow thrust-augmenting nozzle 23 and drainage tube 11 all are that the branch multilayer is horizontal from top to bottom, each layer is established at least 2 Parallel airflow thrust-augmenting nozzles 23 and drainage tube 11, the outlet of every layer Parallel airflow thrust-augmenting nozzle 23, just enter the edge of a plurality of trays one side of horizontal placement, drainage tube 11 is in the opposite side of a plurality of trays of horizontal placement, the correspondence position that parallels one by one with Parallel airflow thrust-augmenting nozzle 23, the direction that one end of drainage tube 11 blows facing to Parallel airflow, the other end compiles chamber 10 with gas and communicates.
The ventilation heat-conducting circulating system that fermentor tank is equipped with is provided with temperature online measurement and control instrument, circulating ventilation pipe watercooler 13 and cooling circulating water system 29, the measurement and control of temperature probe of temperature online measurement and control instrument joins with measurement and control of temperature probe interface 35, the water outlet of cooling circulating water system 29 and water-in join by the water-in 21 and the water outlet 22 of magnetic valve and circulating ventilation pipe watercooler 13 respectively, and the magnetic valve of water-in and water outlet is controlled by the temperature online measurement and control instrument.
The ventilation oxygen supply recycle system that fermentor tank is equipped with is provided with oxygen on-line monitoring device, sterile air system 28, sterile air interface 38 and oxygen on-line monitoring interface 9; The observing and controlling probe of oxygen on-line monitoring device and oxygen on-line monitoring interface 9 join, and the outlet of sterile air system 28 and sterile air interface 38 are connected by magnetic valve, and the switch of magnetic valve is controlled by oxygen on-line monitoring device; When oxygen-consumption was too high in the fermentor tank, the magnetic valve of in time opening sterile air interface 38 automatically charged into fresh sterile air, carries out the forced ventilation circulation simultaneously the oxygen concentration in the fermentor tank is elevated near normal range.
Described metal plate structure is the inner frame of thin stainless steel plate shell and stainless steel angle steel system.
What described resistant to elevated temperatures sealing-ring was selected for use is silicone rubber O-ring, teflin ring etc.
Below provide the example of the Window for ventilation formula solid state fermentation of the present utility model system production of cellulose enzyme shown in employing Fig. 1~6.
By Fig. 1~6 as can be known, Window for ventilation formula solid state fermentation of the present utility model system, comprise between the control of Window for ventilation formula solid-state fermenter 1, solid state fermentation 2 and moistureproof drive-motor 3, Window for ventilation formula solid-state fermenter 1 is between solid state fermentation control in 2,2 have set up air themperature regulation device 26, atmospheric moisture regulation device 27, sterile air system 28 and cooling circulating water system 29 between solid state fermentation control, temperature and humidity in the Window for ventilation solid-state fermenter 1 is controlled by 2 temperature and humidity between the control of regulation and control solid state fermentation.Window for ventilation formula solid-state fermenter 1 is the tank body of the rectangular parallelepiped of a thin stainless steel plate structure, the front of tank body is a door leaf 15, preceding door leaf 15 and rear wall are provided with at least 2 Window for ventilation 18, Window for ventilation formula solid-state fermenter 1 outer vortex air pump 5, circulating ventilation pipe 8, the circulating ventilation pipe watercooler 13 that is provided with the magnetic force couple drive.The circulating ventilation pipe is provided with oxygen on-line monitoring interface 9, sterile air interface 38 and temp probe interface 35 and moisture probe interface 36.Circulating ventilation pipe watercooler 13 is provided with water coolant water inlet interface 21 and water outlet interface 22.Four pin of Window for ventilation formula solid-state fermenter bottom are equipped with the roller 37 of bearing, so that move.Be provided with gas distribution chamber 24, Parallel airflow thrust-augmenting nozzle 23, drainage tube 11, gas in the Window for ventilation formula solid-state fermenter 1 and compile chamber 10 and tray frame 14 movably, but tray frame 14 horizontal positioned multilayer from top to bottom fills the tray 12 of solid medium, and tray frame 14 can freely load and unload with handling machinery together with the tray 12 that is contained on the tray frame.Moistureproof drive-motor 3 is connected with vortex air pump 5 fast-assemblings of magnetic force couple drive by convex-concave shaft joint 4.
Between the control of Window for ventilation formula solid state fermentation system solid state fermentation 2, can be divided at least 2 little, can place 2 Window for ventilation formula solid-state fermenters at least for each little and ferment simultaneously, can realize like this that modularization enlarges to produce.
The button fly front fan 15 of Window for ventilation formula solid-state fermenter 1, it can be the single open-door fan, the double door fan or the fan that opens the door more, on the doorframe 16 seal groove is arranged, and resistant to elevated temperatures sealing-ring is housed, by hinge 17 door leaf is installed on the doorframe 16, when door is closed, realizes tightening seal by the resistant to elevated temperatures silicon sealing-ring that upwards turns over screw 19, door lock 20 down and be installed on the doorframe.
The Window for ventilation 18 of Window for ventilation formula solid-state fermenter 1 is the rectangle of band fillet, circle or other shape, each Window for ventilation be equipped with at least 1 to 0.3 μ m micro particle filtering efficient up to 99.97% respiratory membrane, respiratory membrane is resistant to elevated temperatures organic membrane or mineral membrane, organic membrane comprises polypropylene screen and poly tetrafluoroethylene etc., mineral membrane is a ceramic fibre film etc., respiratory membrane can be the hybrid films that single or multiple lift is formed, respiratory membrane can allow air and airborne water molecules freely diffuse through, airborne assorted bacterium and spore thereof can not pass through, respiratory membrane is by the stainless steel pressure ring, resistant to elevated temperatures sealing-ring, the screw tightening seal is installed on the window, and what resistant to elevated temperatures sealing-ring was selected for use is silicone rubber O-ring.
Vortex air pump 5, circulating ventilation pipe 8, circulating ventilation pipe watercooler 13, gas distribution chamber 24, Parallel airflow thrust-augmenting nozzle 23, drainage tube 11 and gas that Window for ventilation formula solid-state fermenter 1 is provided with successively by the magnetic force couple drive compile the forced ventilation oxygen supply heat-conducting circulating system of forming chamber 10, flow through the Parallel airflow bundle that each layer horizontal positioned fills solid medium tray 12 surfaces in order to make, its fixed air channel is all arranged, Parallel airflow thrust-augmenting nozzle 23 and drainage tube 11 all are that the branch multilayer is horizontal from top to bottom, at least 2 on each layer.The outlet of every layer of Parallel airflow thrust-augmenting nozzle 23 just enters the edge of a plurality of trays one side of horizontal placement, an opposite side that is in a plurality of trays of horizontal placement of drainage tube 11, the correspondence position that parallels one by one with the Parallel airflow thrust-augmenting nozzle.The other end level and the gas of drainage tube 11 compiles chamber 10 and is communicated with.
The ventilation heat-conducting circulating system that the fermentor tank of above-mentioned forced ventilation oxygen supply heat-conducting circulating system is equipped with, form by temperature online measurement and control instrument, circulating ventilation pipe watercooler 13 and cooling circulating water system 29, the measurement and control of temperature probe of temperature online measurement and control instrument joins with temp probe interface 35, and the water outlet of cooling circulating water system 29 and water-in join by the water coolant water inlet interface 21 and the water coolant water outlet interface 22 of magnetic valve and circulating ventilation pipe watercooler 13 respectively; The magnetic valve of water inlet interface and water outlet interface is controlled by the temperature online measurement and control instrument; The biological heat that produces when the fermentor tank internal cause makes temperature surpass going up in limited time of control rapidly, opens the magnetic valve of water inlet of circulating ventilation pipe watercooler and water outlet automatically, by the forced ventilation circulation temperature in the fermentor tank is reduced to below the controlling valu.
The ventilation oxygen supply recycle system that the fermentor tank of above-mentioned forced ventilation oxygen supply heat-conducting circulating system is equipped with is made up of oxygen on-line monitoring device, sterile air system 28, sterile air interface 38 and oxygen on-line monitoring interface 9; The observing and controlling probe of oxygen on-line monitoring device and oxygen on-line monitoring interface 9 join, and the outlet of sterile air system 28 and sterile air interface 38 are connected by magnetic valve, and the switch of magnetic valve is controlled by oxygen on-line monitoring device.When oxygen-consumption was too high in the fermentor tank, the magnetic valve of in time opening sterile air interface 38 charged into fresh sterile air, carries out the forced ventilation circulation simultaneously the oxygen concentration in the fermentor tank is elevated near normal range.
The bacterial classification that present embodiment uses is Trichodermareesei (Trichoderma reesel) 6LZ11, and registering on the books is numbered CGMCCNo.3007.Fermented substrate is that straw powder, wheat bran, urea, sulfuric acid amine, sal epsom, potassium primary phosphate, dipotassium hydrogen phosphate, solid-to-liquid ratio are 1: (1.5~2.0), pH is 4.6~6.5.Fermentation condition is 28~30 ℃ of temperature, relative humidity 88%~95%, and oxygen content is 18%~21% in jar.
Below provide the technical process of production:
(1) batching sabot.By the formulated solid-state fermentation culture medium and the tray of packing into, thickness is 3~5cm, then the tray 12 that substratum is housed is put into tray frame 14, again with handling machinery with in its Window for ventilation formula solid-state fermenter 1 of packing into, door shuts, and unloads the moistureproof drive-motor 3 of magnetic force of vortex air pump;
(2) sterilization and cooling.(if coupled magnets uses is resistant to elevated temperatures SmCo magnet during sterilization, as long as then unload the drive-motor 3 of vortex air pump, if coupled magnets is used is can not resistant to elevated temperatures ndfeb magnet, then must connect magnetic force coupling vortex air pump 5 and unload, carry out independent normal temperature chemosterilization, after the sterilization, again load onto at sterilisable chamber) solid-state fermenter 1 of substratum tray will be housed, push in the vapor sterilization pot, behind 121~123 ℃ of sterilization 40~60min, be cooled to nearly room temperature;
(3) inoculation and fermentation culture.The Window for ventilation formula solid-state fermenter that is cooled to nearly room temperature with sterilizing, after buffer zone and the pouring of aseptic wind, enter 100 grades of transfer rooms with the inoculation of quick inoculation system after, the probe and the interface 9 of oxygen on-line monitoring device are joined, humiture observing and controlling probe is connected respectively with interface 35,36, solid-state fermenter 1 is changed over to solid state fermentation again and 2 carries out fermentation culture between controlling; During fermentation culture, load onto the moistureproof drive-motor 3 of vortex air pump, by temperature and humidity TT﹠ C system 26 and 27,2 temperature and humidity between the control of regulation and control solid state fermentation, interface 21,22 and cooling water recirculation system 29 are joined, interface 38 joins with sterile air system 28, and all starts the Controlling System of solid state fermentation, fermentation time 5~6 days.
4. product drying.After fermentation is finished, Window for ventilation formula solid-state fermenter 1 sent into carry out dry packing between product vacuum-drying.
5. radiation sterilization.The product of dry packing, 60The Co radiation sterilization.
China cellulase preparation light industry standard QB2585-2003 regulation: the CMCA-DNS enzyme activity is 2000u/g, in pilot scale, and 6 batches of continuous production, average CMCA-DNS enzyme activity is 6142u/g, is up to 8006u/g.

Claims (10)

1. Window for ventilation formula solid state fermentation system, be provided with between Window for ventilation formula solid-state fermenter, solid state fermentation control and moistureproof drive-motor, it is characterized in that, in Window for ventilation formula solid-state fermenter is located between solid state fermentation control, sets up the air themperature regulation device that is useful between the control of regulation and control solid state fermentation between solid state fermentation control, be used to regulate and control atmospheric moisture regulation device, sterile air system and cooling circulating water system between solid state fermentation control; Window for ventilation formula solid-state fermenter is the cuboid tank body of a sheet metal structure, and Window for ventilation formula solid-state fermenter is provided with at least 1 button fly front fan, is respectively equipped with at least 2 Window for ventilation on the rear wall of button fly front fan and Window for ventilation formula solid-state fermenter; Window for ventilation formula solid-state fermenter is provided with vortex air pump, circulating ventilation pipe and the circulating ventilation pipe watercooler of magnetic force couple drive outward, the circulating ventilation pipe is provided with oxygen on-line monitoring interface, sterile air interface, temp probe interface and moisture probe interface, circulating ventilation pipe watercooler is provided with water coolant water inlet interface and water coolant water outlet interface, and 4 pin of Window for ventilation formula solid state fermentation pot bottom are provided with the roller of bearing; Be provided with gas distribution chamber, Parallel airflow thrust-augmenting nozzle, drainage tube, gas in the Window for ventilation formula solid-state fermenter and compile chamber and tray frame movably, but tray frame horizontal positioned multilayer from top to bottom fills the tray of solid medium, and moistureproof drive-motor is connected with magnetic force coupled vortex air pump fast-assembling by the convex-concave shaft joint.
2. Window for ventilation formula solid state fermentation as claimed in claim 1 system is characterized in that being divided at least 2 little, at least 2 Window for ventilation formulas of each little placement solid-state fermenter between described solid state fermentation control.
3. Window for ventilation formula solid state fermentation as claimed in claim 1 system, the button fly front fan that it is characterized in that described Window for ventilation formula solid-state fermenter is the single open-door fan, the double door fan or the fan that opens the door more, the doorframe of button fly front fan is provided with seal groove, seal groove is provided with sealing-ring, and the button fly front fan pass is crossed hinge and is installed on the doorframe.
4. Window for ventilation formula solid state fermentation as claimed in claim 1 system is characterized in that rectangle Window for ventilation or the circular Window for ventilation of the Window for ventilation of described Window for ventilation formula solid-state fermenter for the band fillet, and each Window for ventilation is equipped with at least 1 respiratory membrane.
5. Window for ventilation formula solid state fermentation as claimed in claim 4 system, it is characterized in that described respiratory membrane for to 0.3 μ m micro particle filtering efficient up to 99.97% respiratory membrane, respiratory membrane is organic membrane or mineral membrane, organic membrane is polypropylene screen or poly tetrafluoroethylene, mineral membrane is the ceramic fibre film, and respiratory membrane is the hybrid films that single or multiple lift is formed.
6. Window for ventilation formula solid state fermentation as claimed in claim 1 system is characterized in that described Window for ventilation formula solid-state fermenter is provided with a ventilation oxygen supply heat-conducting circulating system of being made up of vortex air pump, circulating ventilation pipe, circulating ventilation pipe watercooler, gas distribution chamber, Parallel airflow thrust-augmenting nozzle, drainage tube and the gas chamber of compiling of magnetic force couple drive successively; Parallel airflow thrust-augmenting nozzle and drainage tube all are that the branch multilayer is horizontal from top to bottom, each layer is established at least 2 Parallel airflow thrust-augmenting nozzles and drainage tube, the outlet of every layer Parallel airflow thrust-augmenting nozzle, just enter the edge of a plurality of trays one side of horizontal placement, drainage tube is in the opposite side of a plurality of trays of horizontal placement, with the correspondence position that the Parallel airflow thrust-augmenting nozzle parallels one by one, the direction that an end of drainage tube blows facing to Parallel airflow, the other end communicates with the gas chamber of compiling.
7. Window for ventilation formula solid state fermentation as claimed in claim 6 system, its feature is that also described ventilation oxygen supply heat-conducting circulating system is provided with temperature online measurement and control instrument, circulating ventilation pipe watercooler and cooling circulating water system, the measurement and control of temperature probe of temperature online measurement and control instrument joins with the measurement and control of temperature probe interface, the water outlet of cooling circulating water system and water-in join by the water-in and the water outlet of magnetic valve and circulating ventilation pipe watercooler respectively, and the magnetic valve of water-in and water outlet is controlled by the temperature online measurement and control instrument.
8. Window for ventilation formula solid state fermentation as claimed in claim 6 system, its feature is that also described ventilation oxygen supply heat-conducting circulating system is provided with oxygen on-line monitoring device, sterile air system, sterile air interface and oxygen on-line monitoring interface; The observing and controlling probe of oxygen on-line monitoring device and oxygen on-line monitoring interface join, and the outlet of sterile air system is connected by magnetic valve with the sterile air interface, and the switch of magnetic valve is controlled by oxygen on-line monitoring device.
9. Window for ventilation formula solid state fermentation as claimed in claim 1 system is characterized in that the cuboid tank body that described cuboid tank body is a metal plate structure, and described cuboid tank body is the inner frame of thin stainless steel plate shell and stainless steel angle steel system.
10. Window for ventilation formula solid state fermentation as claimed in claim 3 system is characterized in that described sealing-ring is silicone rubber O-ring or teflin ring.
CN 200920181811 2009-12-16 2009-12-16 Breathing window type solid state fermentation system Expired - Lifetime CN201605269U (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102093950A (en) * 2010-12-15 2011-06-15 北京科技大学 Ventilation-humidification coupled solid-state fermentation tank and fermentation process thereof
CN104531521A (en) * 2014-12-12 2015-04-22 福建农大科技开发总公司 Shallow dish type solid fermenting equipment
CN104845877A (en) * 2015-04-17 2015-08-19 南京工业大学 Solid-state fermentation reactor and application thereof
CN106282009A (en) * 2016-11-10 2017-01-04 山东省科学院生态研究所 A kind of solid materials fermenting device
CN107603869A (en) * 2017-10-28 2018-01-19 沂源康源生物科技有限公司 Automate solid-state fermentation tank
CN110029054A (en) * 2019-04-25 2019-07-19 宁夏正旺农科产业发展集团有限责任公司 Solid state fermentation high activity complex enzyme for feed fermentation system
CN115197819A (en) * 2022-07-25 2022-10-18 广州美中生物科技有限公司 Solid-state-simulated fermentation device and solid-state-simulated fermentation method

Cited By (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102093950A (en) * 2010-12-15 2011-06-15 北京科技大学 Ventilation-humidification coupled solid-state fermentation tank and fermentation process thereof
CN102093950B (en) * 2010-12-15 2012-11-07 北京科技大学 Ventilation-humidification coupled solid-state fermentation tank and fermentation process thereof
CN104531521A (en) * 2014-12-12 2015-04-22 福建农大科技开发总公司 Shallow dish type solid fermenting equipment
CN104845877A (en) * 2015-04-17 2015-08-19 南京工业大学 Solid-state fermentation reactor and application thereof
CN106282009A (en) * 2016-11-10 2017-01-04 山东省科学院生态研究所 A kind of solid materials fermenting device
CN107603869A (en) * 2017-10-28 2018-01-19 沂源康源生物科技有限公司 Automate solid-state fermentation tank
CN110029054A (en) * 2019-04-25 2019-07-19 宁夏正旺农科产业发展集团有限责任公司 Solid state fermentation high activity complex enzyme for feed fermentation system
CN115197819A (en) * 2022-07-25 2022-10-18 广州美中生物科技有限公司 Solid-state-simulated fermentation device and solid-state-simulated fermentation method

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