CN205590713U - Formula cell culture system is filled to three -dimensional - Google Patents
Formula cell culture system is filled to three -dimensional Download PDFInfo
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- CN205590713U CN205590713U CN201620383300.7U CN201620383300U CN205590713U CN 205590713 U CN205590713 U CN 205590713U CN 201620383300 U CN201620383300 U CN 201620383300U CN 205590713 U CN205590713 U CN 205590713U
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- reactor
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- cell culture
- mounting groove
- storage tube
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- 238000004113 cell culture Methods 0.000 title claims abstract description 39
- 239000007788 liquid Substances 0.000 claims abstract description 47
- 239000002699 waste material Substances 0.000 claims abstract description 41
- 239000000463 material Substances 0.000 claims abstract description 34
- 238000010438 heat treatment Methods 0.000 claims abstract description 20
- 238000002347 injection Methods 0.000 claims abstract description 15
- 239000007924 injection Substances 0.000 claims abstract description 15
- 238000003860 storage Methods 0.000 claims description 57
- NJPPVKZQTLUDBO-UHFFFAOYSA-N novaluron Chemical compound C1=C(Cl)C(OC(F)(F)C(OC(F)(F)F)F)=CC=C1NC(=O)NC(=O)C1=C(F)C=CC=C1F NJPPVKZQTLUDBO-UHFFFAOYSA-N 0.000 claims description 46
- 239000012531 culture fluid Substances 0.000 claims description 34
- 230000010412 perfusion Effects 0.000 claims description 26
- 239000010808 liquid waste Substances 0.000 claims description 16
- 238000012258 culturing Methods 0.000 claims description 14
- 238000001514 detection method Methods 0.000 claims description 14
- 238000007789 sealing Methods 0.000 claims description 11
- 239000004065 semiconductor Substances 0.000 claims description 8
- 239000000243 solution Substances 0.000 abstract description 4
- 238000012832 cell culture technique Methods 0.000 abstract 1
- 239000007789 gas Substances 0.000 description 53
- 238000005057 refrigeration Methods 0.000 description 8
- 238000002474 experimental method Methods 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 230000003287 optical effect Effects 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 230000003068 static effect Effects 0.000 description 3
- 238000012549 training Methods 0.000 description 3
- 238000012546 transfer Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 239000012930 cell culture fluid Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004060 metabolic process Effects 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000003044 adaptive effect Effects 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000001976 enzyme digestion Methods 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000011347 resin Substances 0.000 description 1
- 229920005989 resin Polymers 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Abstract
An embodiment of the utility model provides a formula cell culture system is filled to three -dimensional relates to the cell culture technique field, has the cultivation platform of stable temperature, gas environment and the culture solution velocity of flow and invents in order to provide one for the cell. A formula cell culture system is filled to three -dimensional, includes: culture solution feeding device, the device include syringe pump subassembly and syringe, and the piston that the syringe pump subassembly can promote the syringe is to the motion of injection direction, the reactor mounting groove that is used for installing the reactor is offered to reactor base, sealed lid that cell culture device, the device include the reactor, made by the heat conduction material and be used for the heating device to the heating of reactor base on the reactor base, be equipped with in the reactor mounting groove to be used for letting in gaseous gas channel, and reactor mounting groove top lid is equipped with sealed lid, waste liquid treatment device, the device include waste liquid memory tube and refrigerating plant, and refrigerating plant can refrigerate to the waste liquid memory tube. The utility model is used for cell culture.
Description
Technical field
This utility model relates to technical field of cell culture, particularly relates to a kind of dimensional perfusion formula thin
Born of the same parents' culture systems.
Background technology
Cell is cultivated and is referred to all In vitro culture, and its implication refers to taking-up group in driven thing live body body
Knit, separate tissue is become unicellular, in analogue body by the method for machinery or the method for enzymic digestion
Under the conditions of physiological environment etc. are the most internal, carry out hatching cultivation, be allowed to existence and grow.
Existing cell training method is divided into two kinds, and one is static culture, generally uses cultivation
Bottle or culture dish, be placed in cell in culture vessel, artificial its production status variable interval of observation
Change liquid, provide sufficient nutrient for cell；Another kind is cultivated for filling type, will cell
It is inoculated in reactor, can be that cell or tissue provides culture fluid to grow for it endlessly.
In the mode of static culture, culture fluid needs periodic replacement, and static culture system can not keep
Environment is constant, and manually-operated efficiency and precision are the highest, and easily pollutes culture environment.
Filling type cultivation can reduce manual operation thus reduce the probability of pollution, but, due to needs even
Continuous constantly providing fresh medium for cell, reactor connects with culture fluid always, be difficult to by
Reactor is placed in the incubator with stable temperature and gaseous environment so that it is around reactor
Temperature and gaseous environment be difficult to keep constant, and push syringe supply cell culture fluid stream
Speed is wayward.
Utility model content
Embodiment of the present utility model provides a kind of dimensional perfusion formula cell culture system, Ke Yiwei
Cell provides a cultivation with stable temperature, gaseous environment and culture fluid flow velocity to put down
Platform.
For reaching above-mentioned purpose, embodiment of the present utility model adopts the following technical scheme that
A kind of dimensional perfusion formula cell culture system, including: culture fluid feedway, described training
Nutrient solution feedway includes syringe pump assembly and the syringe being installed on described syringe pump assembly,
Described syringe pump assembly can promote the piston of described syringe to move to injection direction；Cell is cultivated
Device, described cell culture system includes reactor, the reactor pedestal that is made from a material that be thermally conductive,
Seal lid and for the heater to the heating of reactor pedestal, described reactor pedestal is opened
Be provided with the reactor mounting groove for installing reactor, be provided with in described reactor mounting groove for
It is passed through the gas flow of gas, described reactor pedestal offers and connects with described gas flow
Air inlet, be covered with above described reactor mounting groove sealing lid, described reactor include training
Support room and the culture fluid input pipe connected with described culturing room and waste liquid outlet tube, described cultivation
Liquid input pipe connects with the outfan of described syringe；Liquid waste treating apparatus, described liquid waste processing
Device includes waste liquid storage tube and refrigerating plant, described waste liquid storage tube and described waste liquid outlet tube
Connection, described waste liquid storage tube can be freezed by described refrigerating plant.
Further, described syringe pump assembly includes: syringe pump；It is fixedly connected on described injection
The firm banking of pump；Syringe rack, the urceolus of syringe can be fixed by described syringe rack,
Described syringe rack is removably connected on described firm banking；Pushing component, described promotion
Assembly is for clamping the piston rod handle of described syringe；Described injection delivery side of pump pushes away with described
Dynamic assembly is fixing to be connected, to promote the piston of described syringe to move to injection direction.
Further, described syringe is multiple, described syringe rack include lower plate,
Train wheel bridge and clamping element, described clamping element applies clamping force can to train wheel bridge and lower plate, to incite somebody to action
The urceolus of multiple described syringes is clamped and fastened between described train wheel bridge and lower plate；Described folder
Tight part includes that knob and elastomeric element, described knob include screw rod and be fixed on the rotation of screw rod one end
Handle hands, described screw rod through described train wheel bridge via after with the screwed hole on described lower plate
Being connected, described elastomeric element is arranged between described rotating handles and described train wheel bridge, institute
State the width more than or equal to described train wheel bridge of the spacing between the syringe of the adjacent both sides of knob.
Further, described reactor pedestal is provided with dense with the gas that described gas flow connects
Degree detection mouth, described gas concentration detection mouth is communicated with gas concentration detection apparatus.
Further, the upper surface of described reactor pedestal offers for installing culture fluid input
The input pipe mounting groove of pipe and for installing the outlet tube mounting groove of waste liquid outlet tube, described instead
Answer device mounting groove between described input pipe mounting groove and outlet tube mounting groove and respectively with institute
State input pipe mounting groove and outlet tube mounting groove is through.
Further, described reactor includes multiple culturing room, the position of described gas flow with
The position of described culturing room is corresponding, be provided with bottom described reactor mounting groove for support described instead
Answer the support protrusion of device so that bottom the bottom of described reactor and described reactor mounting groove it
Between form described gas flow.
Further, described heater is to be arranged on the heating below described reactor pedestal
Plate, described heating plate is fitted with described reactor pedestal lower surface.
Further, described liquid waste treating apparatus also includes the storage for installing waste liquid storage tube
Pipe mounting box, a described storage tube mounting box at least sidewall is made from a material that be thermally conductive, around institute
Stating storage tube mounting box and be provided around heat-barrier material, described refrigerating plant is arranged at described storage
On the wall outer surface that pipe mounting box is made from a material that be thermally conductive.
Further, described refrigerating plant is semiconductor chilling plate, and described quasiconductor includes system
Huyashi-chuuka (cold chinese-style noodles) and radiating surface, described chill surface contacts with described storage tube mounting box wall, described heat radiation
Face connects radiator；Described liquid waste treating apparatus also includes being surrounded box like structure by heat-barrier material
Shell, and described shell one side wall surface arrange the described liquid waste treating apparatus of connection inside and outside
Fan, be formed with air convection channel between described shell and described storage tube mounting box, institute
Stating radiator and be positioned at described air convection channel porch, described fan is positioned at described cross-ventilation
Channel exit.
Further, described storage tube mounting box top is provided with opening, described opening part be provided with by
The lid that heat-barrier material is made, described opening can be sealed by described lid, and described lid offers
The through hole that connect internal with described storage tube mounting box, described through hole is used for installing described waste liquid and deposits
Chu Guan.
A kind of dimensional perfusion formula cell culture system that this utility model embodiment provides, owing to setting
Put culture fluid feedway, use syringe pump assembly and the injection being installed on syringe pump assembly
Device supplies culture fluid, is moved to injection direction by syringe pump components drive syringe piston,
Can be that cell provides the culture fluid input stablizing flow velocity；In cell culture system, it is provided with
The reactor pedestal being made from a material that be thermally conductive, and for reactor pedestal heating is added hot charging
Putting, reactor is mountable in the reactor mounting groove on reactor pedestal, passes through heater
Reacting by heating device pedestal, reactor pedestal transfers heat to reactor, makes reactor to ensure
Stable temperature, meanwhile, is covered with sealing lid above reactor mounting groove, and reactor is installed
It is provided with the gas flow for being passed through gas in groove, reactor pedestal offers and gas flow
The air inlet of connection, so, the gaseous environment around reactor seals with extraneous, can be from air inlet
Mouth is passed through the gas of needs, makes the gaseous environment around reactor be in stable state；Also set
Put liquid waste treating apparatus, can be used to collect the metabolism thing of cell, for meeting some to carefully
The experiment of born of the same parents' metabolism thing research, and in order to enable well to preserve, being also provided with can be to waste liquid
The refrigerating plant of storage tube refrigeration.Thus, it is possible to provide a kind of have stable temperature, Ring
Border and the culture systems of culture fluid flow velocity.
Accompanying drawing explanation
In order to be illustrated more clearly that this utility model embodiment or technical scheme of the prior art,
The accompanying drawing used required in embodiment or description of the prior art will be briefly described below,
It should be evident that the accompanying drawing in describing below is only embodiments more of the present utility model, right
From the point of view of those of ordinary skill in the art, on the premise of not paying creative work, it is also possible to
Other accompanying drawing is obtained according to these accompanying drawings.
The knot of the dimensional perfusion formula cell culture system that Fig. 1 provides for this utility model embodiment
Structure schematic diagram；
In the dimensional perfusion formula cell culture system that Fig. 2 provides for this utility model embodiment anti-
Answer the structural representation of device pedestal；
In the dimensional perfusion formula cell culture system that Fig. 3 provides for this utility model embodiment anti-
Answer the structural representation of device；
The dimensional perfusion formula cell culture system that Fig. 4 provides for this utility model embodiment is trained
The structural representation of nutrient solution feedway；
The dimensional perfusion formula cell culture system that Fig. 5 provides for this utility model embodiment is noted
Emitter support is installed and the top view on firm banking；
The dimensional perfusion formula cell culture system that Fig. 6 provides for this utility model embodiment is noted
The structural representation of emitter support；
In the dimensional perfusion formula cell culture system that Fig. 7 provides for this utility model embodiment thin
The decomposition texture schematic diagram of born of the same parents' culture apparatus；
In the dimensional perfusion formula cell culture system that Fig. 8 provides for this utility model embodiment close
The structural representation of capping；
The dimensional perfusion formula cell culture system that Fig. 9 provides for this utility model embodiment is deposited
The structural representation of the lid on storage pipe mounting box.
Detailed description of the invention
Below in conjunction with the accompanying drawing in this utility model embodiment, in this utility model embodiment
Technical scheme be clearly and completely described, it is clear that described embodiment be only this
The a part of embodiment of utility model rather than whole embodiments.Based in this utility model
Embodiment, those of ordinary skill in the art are obtained under not making creative work premise
Every other embodiment, broadly falls into the scope of this utility model protection.
In description of the present utility model, it is to be understood that term " " center ", " on ",
D score, "front", "rear", "left", "right", " vertically ", " level ",
" push up ", " end ", " interior ", the orientation of the instruction such as " outward " or position relationship are based on attached
Orientation shown in figure or position relationship, be for only for ease of description this utility model and simplification is retouched
State rather than indicate or imply that the device of indication or element must have specific orientation, Yi Te
Fixed azimuth configuration and operation, therefore it is not intended that to restriction of the present utility model.
In description of the present utility model, it should be noted that unless otherwise clear and definite regulation and
Limiting, term " is installed ", " being connected ", " connection " should be interpreted broadly, such as, and can
To be fixing connection, it is also possible to be to removably connect, or be integrally connected；For this area
For those of ordinary skill, above-mentioned term tool in this utility model can be understood with concrete condition
Body implication.
Embodiment of the present utility model provides a kind of dimensional perfusion formula cell culture system, such as Fig. 1
Shown in, including: culture fluid feedway 1, culture fluid feedway 1 includes syringe pump assembly
2 and the syringe 3 that is installed on syringe pump assembly 2, syringe pump assembly 2 can pushing syringe
The piston of 3 moves to injection direction；Cell culture system 4, cell culture system 4 includes instead
The reactor pedestal 6, the sealing that answer device 5, are made from a material that be thermally conductive cover 7 and for reaction
Device pedestal heating heater 8, as in figure 2 it is shown, offer on reactor pedestal 6 for
The reactor mounting groove 61 of reactor 5 is installed, is provided with for being passed through in reactor mounting groove 61
The gas flow of gas, reactor pedestal 6 offers the air inlet connected with gas flow
62, it is covered with sealing lid 7 above reactor mounting groove 61, as it is shown on figure 3, reactor 5
The culture fluid input pipe 52 including culturing room 51 and connected with culturing room 51 and waste liquid output
Pipe 53, culture fluid input pipe 52 connects with the outfan of syringe 3；As it is shown in figure 1, it is useless
Liquid processing device 9, liquid waste treating apparatus includes waste liquid storage tube 91 and refrigerating plant 92, useless
Liquid storage tube 91 connects with waste liquid outlet tube 53, and refrigerating plant 92 can be to waste liquid storage tube 91
Refrigeration.
This utility model embodiment provide a kind of dimensional perfusion formula cell culture system, as Fig. 1,
2, shown in 3, owing to being provided with culture fluid feedway 1, use syringe pump assembly 2 and install
Syringe 3 on syringe pump assembly 2 supplies culture fluid, is promoted by syringe pump assembly 2
Syringe 3 piston moves to injection direction, can be that cell provides the culture fluid stablizing flow velocity defeated
Enter；In cell culture system 4, it is provided with the reactor pedestal 6 being made from a material that be thermally conductive,
And for the heater 8 to reactor pedestal 6 heating, reactor 5 is mountable to reaction
In reactor mounting groove 61 on device pedestal 6, by heater 8 reacting by heating device pedestal
6, reactor pedestal 6 transfers heat to reactor 5, makes reactor 5 to ensure stable
Temperature, meanwhile, is covered with sealing lid 7 above reactor mounting groove 61, and reactor is installed
It is provided with the gas flow for being passed through gas in groove 61, reactor pedestal 6 offers and gas
The air inlet 62 of body flow passage, so, the gaseous environment around reactor 5 is close with the external world
Envelope, can be passed through the gas of needs, make at the gaseous environment around reactor 5 from air inlet 62
In stable state；It is also provided with liquid waste treating apparatus 9, can be used to collect the new old generation of cell
Thank to thing, some experiments to the research of cell metabolism thing can be met, in order to enable well to preserve,
Being also provided with can be to the refrigerating plant 92 of waste liquid storage tube 91 refrigeration.Thus, it is possible to provide a kind of
There is stable temperature, gaseous environment and the culture systems of culture fluid flow velocity.
Due to the finite capacity of syringe, in use for some time, the culture fluid in syringe
There will be the situation of deficiency, be now accomplished by changing syringe, in order to make syringe be easier to more
Changing, as shown in Figure 4,5, 6, the syringe pump assembly 2 of culture fluid feedway 1 includes: note
Penetrate pump 21 (not shown)；It is fixedly connected on the firm banking 22 of syringe pump 21；Injection
Device support 23, the urceolus of syringe 3 can be fixed by syringe rack 23, syringe rack 23
It is removably connected on firm banking 22；Pushing component 24, pushing component 24 is used for clamping
The piston rod handle of syringe 3；The outfan of syringe pump 21 is fixing with pushing component 24 to be connected,
Move to injection direction with the piston of pushing syringe 3.Owing to syringe rack 23 can will be noted
The urceolus of emitter 3 is fixed, and syringe rack 23 is removably connected on firm banking 22,
When syringe 3 changed by needs, syringe 3 can be taken off together with syringe rack 23,
Change syringe 3 again, after having changed syringe 3, by the syringe rack with syringe 3
23 are arranged on firm banking 22, complete the replacing of syringe 3.And, in order to improve effect
Rate, can make multiple syringe rack 23 removably connected with firm banking 22, work as needs
When changing syringe 3, in advance syringe to be installed can be arranged on another syringe rack
On, then it is changed with the syringe rack equipped with syringe to be replaced, injection can be completed
The replacing of device 3.
In Cell infusion is cultivated, the most once cultivation can arrange multiple culturing room 51 and carries out
Experiment or production, therefore, in order to this utility model embodiment can be that multiple culturing room 51 supplies
To culture fluid, as shown in Figure 4,5, 6, syringe 3 is multiple, and syringe rack 23 wraps
Including train wheel bridge 231, lower plate 232 and clamping element, clamping element can give train wheel bridge 231 and lower folder
Plate 232 applies clamping force, is clamped and fastened on train wheel bridge 231 with the urceolus by multiple syringes 3
And between lower plate 232；Clamping element includes knob 233 and elastomeric element 234, knob 233
Including screw rod and the rotating handles being fixed on screw rod one end, screw rod through after the via of train wheel bridge with
Screwed hole on lower plate is connected, and elastomeric element 234 is arranged at rotating handles and train wheel bridge
Between 231, the spacing between the syringe 3 of the adjacent both sides of knob 233 is more than or equal to upper folder
The width of plate 231.
It should be noted that as shown in Figure 4,5, 6, the clamping element of syringe rack 23 can
Clamping force is applied, with the outer collet chuck by multiple syringes 3 to train wheel bridge 231 and lower plate 232
Holding and be fixed between train wheel bridge 231 and lower plate 232, its implementation can be multiple, example
As, clamping element is screw, can fix, train wheel bridge 231 and lower plate 232 by screw
Rotating, train wheel bridge 231 is extruded by screw towards lower plate 232, give train wheel bridge 231 and under
Clamping plate 232 apply clamping force, make the urceolus of syringe 3 by train wheel bridge 231 and lower plate 232
Grip；Or, as shown in Figure 4, clamping element includes knob 233 and elastomeric element 234,
Knob 233 includes screw rod and is fixed on the rotating handles of screw rod one end, and screw rod passes train wheel bridge
Being connected with the screwed hole on lower plate 232 after the via of 231, elastomeric element 234 is arranged
Between rotating handles and train wheel bridge 231, between the syringe of the adjacent both sides of knob 233 between
Away from the width more than or equal to train wheel bridge 231.Owing to the rotating handles of knob 233 is solid with screw rod
Fixed connection, elastomeric element 234 is arranged between the rotating handles of knob 233 and train wheel bridge 231,
And the screw rod of knob 233 is connected through train wheel bridge 231 with lower plate 232, therefore, elastic
Part 234 gives train wheel bridge 231 1 elastic forces towards lower plate 232, makes syringe 3
Urceolus is gripped by train wheel bridge 231 and lower plate 232, by rotating handles rotary screw,
Distance between rotating handles and the lower plate 232 of adjustment knobs 233, can regulate its bullet
The size of property power.Comparatively speaking, with reference to Fig. 4,5,6, the side of elastomeric element 234 is set
Formula, when changing syringe 3, can not dismantle train wheel bridge 231, first by train wheel bridge 231 vertically to
On lift, then half-twist, be placed between the syringe 3 of the adjacent both sides of knob 233,
And, as shown in Figure 4 and Figure 5, the spacing between the syringe 3 of the adjacent both sides of knob 233
N is more than or equal to the width M of train wheel bridge 231.
In order to different requirement of experiment or different cells cultivate demand, in addition it is also necessary to control reactor
The content of the various gases such as 5 surrounding carbon dioxide, so, the gas being passed through gas flow is permissible
Selection oxygen, nitrogen, carbon dioxide or the mixed gas of these three, further, in order to
The content of gases various in the gas around reactor 5 is carried out more precise control, such as Fig. 2
Shown in Fig. 7, reactor pedestal 6 offers gas concentration detection mouth 63, gas concentration
Detection mouth 63 is communicated with gas concentration detection apparatus 66.Gas concentration detection mouth 63 and gas
Concentration detection apparatus connects, and can detect containing of various gases in the gas around reactor 5
Amount, and then make and regulating targetedly.Wherein, gas concentration detection apparatus 66 can use
Solid-state electrolyte sensor or optical pickocff, comparatively speaking, the stability of optical pickocff
Higher with precision, it is therefore preferable that use optical pickocff.
It should be noted that in order to realize reactor ambient gas concentration is detected, can be
At gas concentration detection mouth 63, gas concentration detector is installed, and gas concentration can be detected mouth 63
Closure, but, structure based on reactor pedestal, gas concentration detector is difficult to install, because of
This, as it is shown in fig. 7, gas concentration detection apparatus 66 include gas concentration detector 661 and
Gas concentration detects the sensing chamber 662 of mouth 63 connection and is used for installing gas concentration detector
The firm banking 663 of 661, sensing chamber 662 is connected with firm banking 663, makes sensing chamber 663
Airtight with the external world, gas concentration detector 661 is in sensing chamber 662.So, can be by gas
Bulk concentration detector 661 is installed and on firm banking 663, then by firm banking 663 and inspection
Survey the fixing connection in room 662, make gas concentration detector 661 be in sensing chamber 662, and inspection
Surveying room 663 airtight with the external world, the gas around reactor 5 connects in sensing chamber 662, gas
Bulk concentration detector 661 detectable gas concentration, and dismounting and change is easier to.
As in figure 2 it is shown, the upper surface of reactor pedestal 6 offers for installing culture fluid input
The input pipe mounting groove 64 of pipe 52 and installing for installing the outlet tube of waste liquid outlet tube 53
Groove 65, reactor mounting groove 61 be positioned at input pipe mounting groove 64 and outlet tube mounting groove 65 it
Between and through with input pipe mounting groove 64 and outlet tube mounting groove 65 respectively.So, culture fluid
Input pipe 52 may be installed in input pipe mounting groove 64, and waste liquid outlet tube 53 may be installed defeated
Go out in pipe mounting groove 65, the convenient operation that cell is cultivated.Simultaneously as reactor pedestal
6 are made from a material that be thermally conductive, and so can heat to cell culture fluid, further, such as Fig. 2
Shown in, the length of input pipe mounting groove 64 suitably can be increased, anti-to improve culture fluid entrance
Answer the pre-heat effect before device 5.
It should be noted that owing to culture fluid input pipe 52 is arranged in input pipe mounting groove 64,
Waste liquid outlet tube 53 is arranged in outlet tube mounting groove 65, in order to avoid culture fluid input pipe 52
With waste liquid outlet tube 53, as shown in Figure 8, and give up near culture fluid input pipe 52 sealing lid 7
The position of liquid outlet tube 53 is provided with breach 71, culture fluid input pipe 52 and waste liquid outlet tube 53 can
Through breach 71, meanwhile, in order to reactor mounting groove 61 will not due to breach 71 with the external world
Connection, is bonded with sealing strip (not shown) on breach 71, and sealing strip can be high density
Spongy body.
In order to meet experiment demand, need once to cultivate substantial amounts of cell or tissue, therefore, as
Shown in Fig. 2 and Fig. 3, reactor 5 includes multiple culturing room 51, the position of gas flow with
The position of culturing room 51 is corresponding, is provided with for supporting reactor bottom reactor mounting groove 61
Support protrusion 611, so that shape between bottom the bottom of reactor 5 and reactor mounting groove 61
Become gas flow.Support protrusion 611 is set bottom reactor mounting groove 61 by reactor 5
Hold up, so, bottom reactor 5 and bottom reactor mounting groove 61 between be the formation of
Gas flow, makes gas may be at around reactor 5.
Heater 8, for heating reactor pedestal 6, can be provided in reactor pedestal
Add heat pipe or heating plate below 6, can use fan that heat blows to reactor pedestal 6 enterprising
Row heating, in order to make the compact conformation of cell cultivation region, and heats is more preferable, such as Fig. 7 institute
Showing, heater 8 is to be arranged on the heating plate below reactor pedestal 6, heating plate and reaction
Device pedestal 6 lower surface is fitted.Heater 8 is set to heating plate, makes compact conformation, and its
Fitting with reactor pedestal 6 lower surface, heats is more preferable.
It should be noted that in order to make the heats to reactor pedestal 6 more preferable, around instead
The wall outer surface answering device pedestal 6 is fitted with heat-conducting plate 81, and described heat-conducting plate 81 adds with described
Hot plate abuts.So, by the heat transfer of heat-conducting plate 81, the sidewall to reactor pedestal 6
It is also carried out heating, makes heats more preferable, and beneficially reactor 5 environment temperature is evenly distributed.
Corresponding multiple culturing room 51, waste liquid storage tube 91 is also multiple, in order to multiple waste liquids
Storage tube 91 freezes simultaneously, as it is shown in figure 1, liquid waste treating apparatus 9 also include for
The storage tube mounting box 93 of waste liquid storage tube 91, storage tube mounting box 93 at least are installed
Sidewall is made from a material that be thermally conductive, and is provided around heat-barrier material around storage tube mounting box 93,
Refrigerating plant 92 is arranged at the wall outer surface that storage tube mounting box 93 is made from a material that be thermally conductive
On, and contact through heat-barrier material with storage tube mounting box 93.By being made from a material that be thermally conductive
Storage tube mounting box 93 provides the space of and extraneous sealing for multiple waste liquid storage tubes 91,
Once multiple waste liquid storage tubes 91 can be freezed.
It should be noted that a storage tube mounting box 93 at least sidewall is made from a material that be thermally conductive,
Can be all to be made from a material that be thermally conductive, or as it is shown in figure 1, only one sidewall 931 is by heat conduction
Material is made, and refrigerating plant is arranged on sidewall 931 outer surface being made from a material that be thermally conductive, phase
Comparatively, owing to the area of refrigeration is limited, in the scheme being all made from a material that be thermally conductive,
Capacity loss may be caused, make refrigerating efficiency reduce, it is therefore preferable that use an only sidewall
531 are made from a material that be thermally conductive, and refrigerating plant is arranged on the sidewall appearance being made from a material that be thermally conductive
Scheme on face.Wherein, Heat Conduction Material can be aluminum material, and meanwhile, other walls can use
Resin material, with rock-steady structure.
Refrigerating plant 92 can be air-cooled, water-cooled or semiconductor chilling plate refrigeration, compares and relatively comes
Saying, the size of semiconductor refrigerating can be the least, lightweight, is not affected by space, and by adjusting
Current size, can change cooling rate, and its speed of action is fast, and service life is long, therefore,
Refrigerating plant 92 is semiconductor chilling plate, including chill surface and radiating surface, as it is shown in figure 1,
Chill surface contacts with storage tube mounting box 93 wall, and radiating surface connects radiator 94.
In order to make the refrigeration of semiconductor chilling plate more preferably, as it is shown in figure 1, liquid waste processing dress
Put 9 shells 95 also including being surrounded box like structure by heat-barrier material, and in shell 95 side
The fan 96 inside and outside connection liquid waste treating apparatus that wall is arranged, shell 95 is installed with storage tube
Being formed with air convection channel between box 93, radiator 94 is positioned at air convection channel porch,
Fan 96 is positioned at air convection channel exit.So, between radiator 94 and fan 96
Form air convection channel, due to the cooling surface contacts of radiator 94 with semiconductor chilling plate,
Can dispel the heat radiating surface faster, the refrigeration making chill surface is more efficient.
Storage tube mounting box 93 seals with extraneous, can be integrated structure, it is also possible to
It is provided with opening, and the lid that opening can be sealed, but, owing to installing waste liquid storage tube
91 need, structure is further opened with the through hole matched with waste liquid storage tube 91, compare and
Speech, after integrated structure through hole sets, is only capable of the adaptive waste liquid that can coordinate with through hole and deposits
Storage pipe 91, it is impossible to change, and the scheme that lid is set can make multiple can with different size give up
The through hole that liquid storage tube 91 coordinates, and then can change flexibly.Therefore, as it is shown in figure 1,
Storage tube mounting box 93 top is provided with opening, and opening part is provided with the lid being made up of heat-barrier material
932, opening can be sealed by lid 932, as it is shown in figure 9, lid 932 offers and stores
The through hole 933 of the internal connection of pipe mounting box 93, through hole 933 is used for installing waste liquid storage tube 91.
So, opening can be sealed by lid 932, makes storage tube mounting box 93 inner space with extraneous
Sealing, and then improve refrigeration, meanwhile, the through hole 933 that lid 932 is arranged can be used to
Waste liquid storage tube 91 is installed.
It should be noted that through hole 933 is the tightst with coordinating of waste liquid storage tube 91
Coordinate or interference fit, be so to keep storage tube mounting box 93 inner space with extraneous
Seal.It addition, lid 932 can be tabular, be covered on opening part, can bonding or clamping,
It is of course also possible to as it is shown in figure 9, lid 932 also includes flat part 9321 and for propping up
The supporting part 9322 of support flat part 9321, the outline of flat part 9321 and the profile of opening
Match, storage tube mounting box 93 can be sealed with extraneous.
These are only detailed description of the invention of the present utility model, but protection domain of the present utility model
It is not limited thereto, the technology that any those skilled in the art disclose at this utility model
In the range of, the change that can readily occur in or replacement, all should contain at protection model of the present utility model
Within enclosing.Therefore, protection domain of the present utility model should be as the criterion with scope of the claims.
Claims (10)
1. a dimensional perfusion formula cell culture system, it is characterised in that including:
Culture fluid feedway, described culture fluid feedway includes syringe pump assembly and the syringe being installed on described syringe pump assembly, and described syringe pump assembly can promote the piston of described syringe to move to injection direction；
Cell culture system, described cell culture system includes reactor, the reactor pedestal being made from a material that be thermally conductive, seal lid and for the heater to the heating of reactor pedestal, the reactor mounting groove for installing reactor is offered on described reactor pedestal, the gas flow for being passed through gas it is provided with in described reactor mounting groove, offer, on described reactor pedestal, the air inlet connected with described gas flow, sealing lid it is covered with above described reactor mounting groove, described reactor includes culturing room and the culture fluid input pipe connected with described culturing room and waste liquid outlet tube, described culture fluid input pipe connects with the outfan of described syringe；
Liquid waste treating apparatus, described liquid waste treating apparatus includes that waste liquid storage tube and refrigerating plant, described waste liquid storage tube connect with described waste liquid outlet tube, and described waste liquid storage tube can be freezed by described refrigerating plant.
Dimensional perfusion formula cell culture system the most according to claim 1, it is characterised in that described syringe pump assembly includes: syringe pump；It is fixedly connected on the firm banking of described syringe pump；Syringe rack, the urceolus of syringe can be fixed by described syringe rack, and described syringe rack is removably connected on described firm banking；Pushing component, described pushing component is for clamping the piston rod handle of described syringe；Described injection delivery side of pump is fixing with described pushing component to be connected, to promote the piston of described syringe to move to injection direction.
Dimensional perfusion formula cell culture system the most according to claim 2, it is characterized in that, described syringe is multiple, described syringe rack includes lower plate, train wheel bridge and clamping element, described clamping element applies clamping force can to train wheel bridge and lower plate, is clamped and fastened between described train wheel bridge and lower plate with the urceolus by multiple described syringes；Described clamping element includes knob and elastomeric element, described knob includes screw rod and is fixed on the rotating handles of screw rod one end, described screw rod is connected with the screwed hole on described lower plate after the via of described train wheel bridge, described elastomeric element is arranged between described rotating handles and described train wheel bridge, and the spacing between the syringe of the adjacent both sides of described knob is more than or equal to the width of described train wheel bridge.
Dimensional perfusion formula cell culture system the most according to claim 1, it is characterised in that described reactor pedestal is provided with the gas concentration detection mouth connected with described gas flow, described gas concentration detection mouth is communicated with gas concentration detection apparatus.
Dimensional perfusion formula cell culture system the most according to claim 1, it is characterized in that, the upper surface of described reactor pedestal offers the input pipe mounting groove for installing culture fluid input pipe and for installing the outlet tube mounting groove of waste liquid outlet tube, and described reactor mounting groove is between described input pipe mounting groove and outlet tube mounting groove and through with described input pipe mounting groove and outlet tube mounting groove respectively.
Dimensional perfusion formula cell culture system the most according to claim 1, it is characterized in that, described reactor includes multiple culturing room, the position of described gas flow is corresponding with the position of described culturing room, the support protrusion for supporting described reactor it is provided with, so that forming described gas flow between bottom the bottom of described reactor and described reactor mounting groove bottom described reactor mounting groove.
Dimensional perfusion formula cell culture system the most according to claim 1, it is characterised in that described heater is to be arranged on the heating plate below described reactor pedestal, described heating plate is fitted with described reactor pedestal lower surface.
Dimensional perfusion formula cell culture system the most according to claim 1, it is characterized in that, described liquid waste treating apparatus also includes the storage tube mounting box for installing waste liquid storage tube, a described storage tube mounting box at least sidewall is made from a material that be thermally conductive, being provided around heat-barrier material around described storage tube mounting box, described refrigerating plant is arranged on the wall outer surface that described storage tube mounting box is made from a material that be thermally conductive.
Dimensional perfusion formula cell culture system the most according to claim 8, it is characterized in that, described refrigerating plant is semiconductor chilling plate, and described semiconductor chilling plate includes chill surface and radiating surface, described chill surface contacts with described storage tube mounting box wall, and described radiating surface connects radiator；Described liquid waste treating apparatus also includes the shell being surrounded box like structure by heat-barrier material, and connect the fan inside and outside described liquid waste treating apparatus what described shell one side wall surface was arranged, it is formed with air convection channel between described shell and described storage tube mounting box, described radiator is positioned at described air convection channel porch, and described fan is positioned at described air convection channel exit.
Dimensional perfusion formula cell culture system the most according to claim 8, it is characterized in that, described storage tube mounting box top is provided with opening, described opening part is provided with the lid being made up of heat-barrier material, described opening can be sealed by described lid, described lid offers the through hole that connect internal with described storage tube mounting box, and described through hole is used for installing described waste liquid storage tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620383300.7U CN205590713U (en) | 2016-04-28 | 2016-04-28 | Formula cell culture system is filled to three -dimensional |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620383300.7U CN205590713U (en) | 2016-04-28 | 2016-04-28 | Formula cell culture system is filled to three -dimensional |
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| Publication Number | Publication Date |
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| CN205590713U true CN205590713U (en) | 2016-09-21 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109679849A (en) * | 2019-01-29 | 2019-04-26 | 四川省肿瘤医院 | A kind of Three-Dimensional Dynamic cell culture system |
| WO2019144717A1 (en) * | 2018-01-23 | 2019-08-01 | 深圳市瀚海基因生物科技有限公司 | Bearing module, nucleic acid loading device and application |
-
2016
- 2016-04-28 CN CN201620383300.7U patent/CN205590713U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019144717A1 (en) * | 2018-01-23 | 2019-08-01 | 深圳市瀚海基因生物科技有限公司 | Bearing module, nucleic acid loading device and application |
| EP3744823A4 (en) * | 2018-01-23 | 2021-10-27 | GeneMind Biosciences Company Limited | Bearing module, nucleic acid loading device and application |
| US11931742B2 (en) | 2018-01-23 | 2024-03-19 | Genemind Biosciences Company Limited | Carrying module, nucleic acid loading device and use thereof |
| CN109679849A (en) * | 2019-01-29 | 2019-04-26 | 四川省肿瘤医院 | A kind of Three-Dimensional Dynamic cell culture system |
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Assignee: Chinese medical equipment sales Co.,Ltd. Assignor: Tianjin Weikai Bioeng Ltd. Contract record no.: 2017120000046 Denomination of utility model: Formula cell culture system is filled to three -dimensional Granted publication date: 20160921 License type: Common License Record date: 20171226 |
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