CN205821355U - A kind of culture plant cell device - Google Patents

Abstract

This utility model provides a kind of culture plant cell device, this technical scheme by prior art is positioned at bottle top, opening up ventilation mouth is arranged at body side, end face is arranged down simultaneously, thus alleviate sedimentation microorganism and bottle implants cell culture system is infected risk.For the terrestial enviornment suitable to plant cell offer, this utility model has tiled palm bark in culture bottle inner bottom surface, plant cell can be grown in field planting thereon, owing to palm bark has the filament of complexity, therefore surface area is bigger, on the one hand the attachment being easy to cell is simultaneously also beneficial to the absorption to culture fluid, thus provides the nutrition of abundance for cell.Additionally, due to palm bark belongs to plant tissue, therefore harmless to cytotoxic, can Reusability.This utility model achieves prominent technique effect with the technological improvement of novelty, and cost is relatively low simultaneously, be easily achieved, and therefore has good promotion prospect.

Description

A kind of culture plant cell device

Technical field

This utility model relates to culture plant cell device technique field, is specifically related to a kind of culture plant cell dress Put.

Background technology

Culture plant cell is in vitro, and callus or other easy scattered tissues are placed in liquid training Support and base carry out concussion cultivation, obtain being dispersed into free suspension cell, make cell proliferation by successive transfer culture, Thus obtain a kind of technology of a large amount of cell colony.According to Object of Development, culture plant cell mainly has unicellular Cultivating, monoploid is cultivated, Protoplast cuhnre etc.；According to culture systems can be divided into suspension culture, liquid culture, Solid culture, Immobilized culture etc..

Owing to plant cell belongs to autotrophic type, therefore in growth course, other cells are had by dissolved oxygen, illumination Requirements at the higher level；And be different from antibacterial, fungus etc. can the microorganism of free living existence in nature, plant is thin Born of the same parents are relatively low to the defensive ability/resistance ability of other microbial infections, therefore should especially focus on dye in culture plant cell technique Bacterium risk.For above reality need, in addition to the condition of culture more excellent in the design of technological operation aspect, training The suitability supporting device affects the culture efficiency of plant cell the most to a great extent, and the plant of prior art is thin The universal existing defects of born of the same parents' culture apparatus.

Additionally, for the plant cell of multiple terrestrial, when prior art finding it cultivated in culture fluid Owing to liquid environment dispersibility is relatively strong, therefore culture density is relatively low, and if carried out terrestrial completely cultivate (Gu Body culture medium culturing), it is difficult to again scale propagation because of nutritious compound absorption inefficiency, asks for this Topic prior art has researcher attempt by the solid content with adsorptivity to grow as the field planting of plant cell Place, although but the such as module such as sponge, foam plastics be provided simultaneously with adsorptivity and can be used for cell field planting, But owing to this compounds may produce genotoxic potential to biology, the most often affect cell growth, it is also possible to Culture fluid is caused to pollute.

Summary of the invention

This utility model aims to the technological deficiency of prior art, it is provided that a kind of culture plant cell device, with Solve the culture plant cell device of prior art and be difficult to while the suitable terrestrial culture environment of simulation the most just In the cell absorption to nutrient.

To be solved in the utility model another technical problem is that the culture plant cell device of prior art is in simulation Toxicity is there is in cell during terrestial enviornment.

For realizing above technical purpose, this utility model by the following technical solutions:

A kind of culture plant cell device, it is characterised in that include vial, feed supplement pipe, discharge duct, seal lid, Permeability cell, filter membrane, uviol lamp, electric heater, ultrasonator, palm bark, wherein feed supplement pipe, discharging Pipe is connected on vial sidewall, and discharge duct is positioned at vial bottom surface, and feed supplement pipe position is higher than discharge duct, Seal cap sealing is connected to vial upper end bottle mouth position, seals lid edge and is attached with sealant tape, permeability cell one Individual end face is connected on vial sidewall, another end face down, the attachment of another end of described permeability cell Having filter membrane, uviol lamp to be fixedly connected on permeability cell inwall, electric heater is fixedly connected on permeability cell outer wall, Ultrasonator is fixedly connected on vial sidewall, and described uviol lamp, electric heater, ultrasonator are respectively Being connected with power supply by wire, palm bark is laid on vial bottom surface, and described palm bark not exclusively covers The bottom surface of vial.

As preferably, vial sidewall connects to be had daylight lamp, described day to turn off the light to be connected with power supply by wire.

As preferably, described sealing lid is plug.

This utility model device in use, adds culture fluid so that culture fluid liquid level and Petiolus Trachycarpi in culture bottle Bark upper surface maintains an equal level or slightly below palm bark upper surface so that palm bark is moisture state, thus for Cell ensures nutrition supply while providing field planting place.

In above technical scheme, this utility model will be positioned at bottle top, opening up saturating in prior art QI KOU is arranged at body side, is arranged down by end face simultaneously, thus alleviates sedimentation microorganism to bottle interplantation Thing cell culture system infect risk.On this basis, this utility model is arranged in the ventilation mouth of carpenter's square shape Uviol lamp, carries out ultraviolet sterilization to the air-flow flowing through ventilation mouth；Meanwhile, outside ventilation mouth, it is provided with electricity to add Hot device, further heat sterilization.Additionally, this utility model is to promote the dissolved oxygen in cultivating system, in bottle side Wall is provided with ultrasonator, by the microscopic motion of liquid in sonic oscillation effect promotion bottle, and then promotes Dissolved oxygen.

For the terrestial enviornment suitable to plant cell offer, this utility model tiles in culture bottle inner bottom surface Palm bark so that plant cell can the growth of field planting thereon, owing to palm bark has the thread knot of complexity Structure, therefore surface area is relatively big, and the attachment being on the one hand easy to cell is simultaneously also beneficial to the absorption to culture fluid, from And be that cell provides sufficient nutrition.Additionally, due to palm bark belongs to plant tissue, therefore to cytotoxic Harmless, can Reusability.This utility model achieves prominent technique effect with the technological improvement of novelty, with Shi Chengben is relatively low, be easily achieved, and therefore has good promotion prospect.

Accompanying drawing explanation

Fig. 1 is the structural representation of this utility model embodiment 1；

In figure:

1, vial 2, feed supplement pipe 3, discharge duct 4, sealing lid

5, permeability cell 6, filter membrane 7, uviol lamp 8, electric heater

9, ultrasonator 10, palm bark

Detailed description of the invention

Hereinafter detailed description of the invention of the present utility model will be described in detail.In order to avoid the most unnecessary Details, in the examples below to belonging to known structure or function will not be described in detail.Following example Used in approximating language can be used for quantitative expression, show can allow in the case of not changing basic function Quantity has certain variation.In addition to being defined, technology used in following example and scientific terminology have and this The identical meanings that utility model one of ordinary skill in the art are commonly understood by.

Embodiment 1

A kind of culture plant cell device, including vial 1, feed supplement pipe 2, discharge duct 3, seals lid 4, thoroughly Trachea 5, filter membrane 6, uviol lamp 7, electric heater 8, ultrasonator 9, palm bark 10, wherein feed supplement Pipe 2, discharge duct 3 are connected on vial 1 sidewall, and discharge duct 3 is positioned at vial 1 bottom surface, mend Material pipe 2 position, higher than discharge duct 3, seals lid 4 and is sealedly connected on vial 1 upper end bottle mouth position, seal lid 4 Edge is attached with sealant tape, and 5 one end faces of permeability cell are connected on vial 1 sidewall, another end face Down, another end of described permeability cell 5 is attached with filter membrane 6, and uviol lamp 7 is fixedly connected on permeability cell On 5 inwalls, electric heater 8 is fixedly connected on permeability cell 5 outer wall, and ultrasonator 9 is fixedly connected on glass On glass bottle 1 sidewall, described uviol lamp 7, electric heater 8, ultrasonator 9 are respectively by wire and power supply Connecting, palm bark 10 is laid on vial 1 bottom surface, the described incomplete cover glass bottle of palm bark 10 The bottom surface of 1.

On the basis of above technical scheme, meet following condition:

Connect on vial 1 sidewall and have daylight lamp, described day to turn off the light to be connected with power supply by wire.

The described lid 4 that seals is plug.

Embodiment 2

A kind of culture plant cell device, including vial 1, feed supplement pipe 2, discharge duct 3, seals lid 4, thoroughly Trachea 5, filter membrane 6, uviol lamp 7, electric heater 8, ultrasonator 9, palm bark 10, wherein feed supplement Pipe 2, discharge duct 3 are connected on vial 1 sidewall, and discharge duct 3 is positioned at vial 1 bottom surface, mend Material pipe 2 position, higher than discharge duct 3, seals lid 4 and is sealedly connected on vial 1 upper end bottle mouth position, seal lid 4 Edge is attached with sealant tape, and 5 one end faces of permeability cell are connected on vial 1 sidewall, another end face Down, another end of described permeability cell 5 is attached with filter membrane 6, and uviol lamp 7 is fixedly connected on permeability cell On 5 inwalls, electric heater 8 is fixedly connected on permeability cell 5 outer wall, and ultrasonator 9 is fixedly connected on glass On glass bottle 1 sidewall, described uviol lamp 7, electric heater 8, ultrasonator 9 are respectively by wire and power supply Connecting, palm bark 10 is laid on vial 1 bottom surface, the described incomplete cover glass bottle of palm bark 10 The bottom surface of 1.

Above embodiment of the present utility model is described in detail, but described content is the most of the present utility model Preferred embodiment, not in order to limit this utility model.All done in application range of the present utility model appoint What amendment, equivalent and improvement etc., within should be included in protection domain of the present utility model.

Claims (2)

1. null1. a culture plant cell device，It is characterized in that including vial (1)，Feed supplement pipe (2)，Discharge duct (3)，Seal lid (4)，Permeability cell (5)，Filter membrane (6)，Uviol lamp (7)，Electric heater (8)，Ultrasonator (9)，Palm bark (10)，Wherein feed supplement pipe (2)、Discharge duct (3) is connected on vial (1) sidewall，Discharge duct (3) is positioned at vial (1) bottom surface，Feed supplement pipe (2) position is higher than discharge duct (3)，Seal lid (4) and be sealedly connected on vial (1) upper end bottle mouth position，Seal lid (4) edge and be attached with sealant tape，(5) end faces of permeability cell are connected on vial (1) sidewall、Another end face is down，Another end of described permeability cell (5) is attached with filter membrane (6)，Uviol lamp (7) is fixedly connected on permeability cell (5) inwall，Electric heater (8) is fixedly connected on permeability cell (5) outer wall，Ultrasonator (9) is fixedly connected on vial (1) sidewall，Described uviol lamp (7)、Electric heater (8)、Ultrasonator (9) is connected with power supply by wire respectively，Palm bark (10) is laid on vial (1) bottom surface，The bottom surface of described palm bark (10) not exclusively cover glass bottle (1).
2. Culture plant cell device the most according to claim 1, it is characterised in that described sealing lid (4) is plug.