CN207519217U - A kind of device for studying allelopathy activity - Google Patents

A kind of device for studying allelopathy activity Download PDF

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Publication number
CN207519217U
CN207519217U CN201721583141.6U CN201721583141U CN207519217U CN 207519217 U CN207519217 U CN 207519217U CN 201721583141 U CN201721583141 U CN 201721583141U CN 207519217 U CN207519217 U CN 207519217U
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China
Prior art keywords
layer container
room
partition board
allelopathy
research
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CN201721583141.6U
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朱书生
叶辰
张俊星
杨敏
刘屹湘
黄惠川
何霞红
梅馨月
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Yunnan Agricultural University
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Yunnan Agricultural University
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Abstract

The utility model is directed in traditional glass cover culturing room in the prior art and is unable to reach aseptic condition, leakproofness is not strong, volatile matter carries miscellaneous bacteria and replaces the problems such as volatile matter is difficult, it provides a kind of sterile, the device of closed research allelopathy activity, by designing double-chamber structure, the plant residue of generation volatile matter is made to be placed on lower floor's volatilization room, the culturing room that volatilization gas passes through void diffusion to upper strata, microbial contamination is excluded to greatest extent, test error caused by container closure and actual operation, so as to be more advantageous to carrying out volatile matter plant, the allelopathic research of volatile matter pathogen and volatile matter insect etc..

Description

A kind of device for studying allelopathy activity
Technical field
The utility model is related to a kind of Interspecific interactions for studying plant volatile mediation, belong to plant protection technology neck Domain, and in particular to it is a kind of research plant-plant, in the interactions such as plant-pathogen, plant-insect the function of volatile matter and The device of mechanism of action.
Background technology
Have in scientific research and study influence of the volatile matter to special object using confined space method.Numerous research works Person sets a closed glass vessel space, and add in simultaneously wherein volatile material and research object (plant, microorganism, Insect), influence of the observation volatile materials to research object, and then analyze have the species of volatile materials by volatile matter with The potential mechanism that research object interacts.
The equipment of tradition research allelopathy volatile matter experiment is a simple closed glass container.This tradition dress Put the problem is that:(1) volatile material and research object are placed jointly in closed vessel, since volatile material can not be into Row high-temperature sterilization, therefore gnotobasis is unable to reach in entire confined space, the interior raw microorganism in volatile material is easily to grinding Study carefully object to have an impact and then generate interference to result of the test;(2) volatile material in studying is often biological living substance Residuum, after a certain period of time i.e. can generate it is rotten, therefore carry out long-time volatile matter biological activity test when need to periodically carry out volatility Material is replaced to ensure function and effect, but this difficulty in conventional containment vessel is larger, and replaces higher dirt every time Contaminate risk;(3) it is mingled with a large amount of microorganisms in volatile matter volatilization process, easily result of the test is made to be interfered;(4) traditional glass Vessel have compared with big limitation, it is difficult to take into account influence of the volatile material to plant, microorganism, insect simultaneously, need according to difference Research object be designed and be transformed, it is both costly and inefficient.
Utility model content
The utility model in traditional glass cover culturing room in the prior art because be unable to reach sterile, leakproofness not by force, The problem of volatile matter carries miscellaneous bacteria and replaces volatile matter difficulty etc. and bring, provides a kind of sterile, closed research allelopathy The device of activity excludes microbial contamination, container closure and test error caused by actual operation, is used for greatest extent Carry out the allelopathic research of volatile matter-plant, volatile matter-pathogen and volatile matter-insect etc..
The device of research allelopathy activity described in the utility model includes volatilization room, partition board and training successively from bottom to top Support room three parts.
The volatilization room is formed by internal layer container is nested with outer layer container, and the diameter of internal layer container is less than outer layer container, makes Double wall cavity is formed between internal layer container outer wall and outer layer container inner wall;The vertical height of the internal layer container is held less than outer layer Device;
The top of the internal layer container sets an air inlet, and the air inlet passes through outer layer container and extends to outer layer container Outside wall, the air inlet extended to outside outer layer container wall and air circulation pump are connected, for the research vegetalization Feel in the device of activity and be passed through air.
Partition board described in the utility model is lamina of septum pellucidum, on the upper edge of volatilization room internal layer container, by partition board There is the hollow cultivation region that an edge projects upwards at upper plate and partition board lower plate composition, the center of the partition board upper plate, can be wherein Research material is placed, cultivation region cross-sectional shape can be round, ellipse or other shapes.
The partition board upper plate and partition board lower plate is respectively provided with two arc-shaped vacancy sections.
Culturing room described in the utility model is mounted and sealed at the bucket of the open at its lower end on volatilization room outer layer container for one Shape container.Culturing room lower face perimeter is equal with volatilization room outer layer container upper edge perimeter so that culturing room can install It is sealed on volatilization room and with volatilization room outer layer container upper edge.
One gas outlet on the upper surface of the culturing room is set, is connected by leather hose with air circulation pump.
The culturing room is preferably heat safe transparent vessel, can ensure the demand of high-temperature sterilization in this way and ensure to plant To the demand of light in object growth course.
The scheme that the utility model solves the technical problem is by designing double-chamber structure, and the plant for making generation volatile matter is residual Body is placed on lower floor's volatilization room, the culturing room that volatilization gas passes through void diffusion to upper strata.
The utility model has the following advantages that:
(1) device described in the utility model in use, can dismantle volatilization room, facilitate volatile matter, not influence to train Support room sterilized space.
(2) gas outlet of culturing room can be used for keeping the skin wet.
(3) volatile matter and the interaction of plant, insect, pathogen and dual factors can be studied.
(4) it is cheap, it can be recycled.
Description of the drawings
Fig. 1 is the schematic diagram of the device of the research allelopathy activity of the utility model.
Fig. 2 is volatilization cell structure schematic diagram.
Fig. 3 is diaphragm structure schematic diagram.
Fig. 4 is partition board impaction state structure diagram.
Fig. 5 is culturing room's structure diagram.
In figure, 1- volatilizations room, 2- partition boards, 3- culturing room, 4- internal layer containers, 5- outer layer containers, 6- folder empty avoidings chamber, 7- air inlets Mouth, 8- partition boards upper plate, 9- partition boards lower plate, 10- vacancy sections, 11- cultivation regions, 12- micropores filter paper, 13- gas outlets.
Specific embodiment
The device of research allelopathy activity described in the utility model is described further below in conjunction with the accompanying drawings, institute It states device and includes volatilization 3 three parts of room 1, partition board 2 and culturing room successively from bottom to top;The volatilization room 1 is by internal layer container 4 and outside The nested composition of layer container 5, the diameter of internal layer container are less than outer layer container, make between 5 inner wall of 4 outer wall of internal layer container and outer layer container Form double wall cavity 6;The vertical height of the internal layer container is less than outer layer container.The top setting one of the internal layer container 4 Air inlet 7, the air inlet 7 passes through outer layer container and extends to outside 5 wall of outer layer container, described to extend to 5 device of outer layer container Air inlet outside wall is connected with air circulation pump, for being passed through air into the device of the research allelopathy activity.
The culturing room 3 is mounted and sealed at the open bulk containers in lower face on volatilization room outer layer container 5 for one.Institute It is equal with volatilization room outer layer container upper edge perimeter to state culturing room's lower face perimeter.One is set on the upper surface of the culturing room Gas outlet 13 is connected by leather hose with air circulation pump.
The partition board 2 be lamina of septum pellucidum, mounted on volatilization room internal layer container 8 upper edge on, by partition board upper plate 8 and every Plate lower plate 9 forms, and has two arc-shaped vacancy sections 10 in partition board upper plate 8 and partition board lower plate 9.The center of partition board upper plate 8 has one The hollow cultivation region 11 that edge projects upwards, cultivation region cross-sectional shape is circle in the specific embodiment, is used in cultivation region In placement research material.The partition board upper plate and partition board lower plate is respectively provided with two arc-shaped vacancy sections.The partition board 2 makes Used time needs place filter paper in partition board upper plate 8 and partition board lower plate 9, then mutually compress partition board upper plate and partition board lower plate, close Envelope is mounted on 1 top of volatilization room.The filter paper was used for the microorganism in air filtering.
The use process of the device of research allelopathy activity described in the utility model is as follows:(1) preceding elder generation is used Sterilization treatment is carried out to the component;(2) allelopathic volatile matter is fitted into volatilization room 1, in partition board upper plate 8 and partition board lower plate 9 Place one 0.22 μm of micropore filter paper 12, (3) by the plant that the culture dish being inoculated with, insect and culture bottle are planted be positioned over every In the cultivation region 11 of plate 2, then partition board 2 is assembled;It compresses and is mounted on the upper edge of volatilization room internal layer container 8;(4) training It supports room 3 and compresses to be sealingly mounted at and volatilize on room, air inlet 7 and gas outlet 13 are connected by leather hose with air circulation pump respectively, Air circulation pump is opened, volatile gases is made to pass through miillpore filter 12 and rise to culturing room 3, while sky can be filtered for air filter film Microorganism in gas reaches sterile culture condition, is cultivated.
If test material is plant, leather hose can be temporarily pulled out, is watered by culturing room gas outlet 13, avoids volatile substance It scatters and disappears.In incubation, volatilization room can be dismantled from lower part, volatile substance is added, does not influence aseptic technique.

Claims (7)

1. a kind of device for studying allelopathy activity, it is characterised in that include volatilization room, partition board and culture successively from bottom to top Room three parts;The volatilization room is formed by internal layer container is nested with outer layer container, internal layer container outer wall and outer layer container inner wall it Between formed double wall cavity;The vertical height of the internal layer container is less than outer layer container;It is indoor that the partition board is mounted on volatilization It on the upper edge of layer container, is made of the partition board upper plate and partition board lower plate that mutually compress, the center of the partition board upper plate has on one side The hollow cultivation region that edge projects upwards, the culturing room are mounted and sealed at the open at its lower end on volatilization room outer layer container for one Bulk containers.
2. the device of research allelopathy activity according to claim 1, it is characterised in that the top of the internal layer container One air inlet is set, and the air inlet passes through outer layer container and extends to outside outer layer container wall, described to extend to outer layer container Air inlet outside wall is connected with air circulation pump.
3. the device of research allelopathy activity according to claim 1, it is characterised in that the partition board is septum pellucidum Plate.
4. the device of the research allelopathy activity according to one of claim 1-3, it is characterised in that on the partition board Plate and partition board lower plate are respectively provided with two arc-shaped vacancy sections.
5. the device of research allelopathy activity according to claim 1, it is characterised in that the culturing room lower face week It is long equal with volatilization room outer layer container upper edge perimeter.
6. the device of research allelopathy activity according to claim 1, it is characterised in that the upper end of the culturing room One gas outlet on face is set, is connected by leather hose with air circulation pump.
7. the device of research allelopathy activity according to claim 1, it is characterised in that the culturing room is resistance to height The transparent vessel of temperature.
CN201721583141.6U 2017-11-23 2017-11-23 A kind of device for studying allelopathy activity Active CN207519217U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201721583141.6U CN207519217U (en) 2017-11-23 2017-11-23 A kind of device for studying allelopathy activity

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201721583141.6U CN207519217U (en) 2017-11-23 2017-11-23 A kind of device for studying allelopathy activity

Publications (1)

Publication Number Publication Date
CN207519217U true CN207519217U (en) 2018-06-22

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111624313A (en) * 2020-06-23 2020-09-04 东北林业大学 Research device and method for allelopathy of volatile organic compounds of plant root system
CN113817798A (en) * 2021-09-29 2021-12-21 广西民族大学 Potted plant detection device and detection method for action of microbial volatile substances on plants

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111624313A (en) * 2020-06-23 2020-09-04 东北林业大学 Research device and method for allelopathy of volatile organic compounds of plant root system
CN113817798A (en) * 2021-09-29 2021-12-21 广西民族大学 Potted plant detection device and detection method for action of microbial volatile substances on plants
CN113817798B (en) * 2021-09-29 2023-07-21 广西民族大学 Potted plant detection device and detection method for plant effect of microorganism volatile substances

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