CN204939495U - A kind of high-throughput plant solid-liquid culture apparatus - Google Patents
A kind of high-throughput plant solid-liquid culture apparatus Download PDFInfo
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- CN204939495U CN204939495U CN201520663789.9U CN201520663789U CN204939495U CN 204939495 U CN204939495 U CN 204939495U CN 201520663789 U CN201520663789 U CN 201520663789U CN 204939495 U CN204939495 U CN 204939495U
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Abstract
The utility model discloses a kind of high-throughput plant solid-liquid culture apparatus, comprise and cultivate box and top cover, described top cover can folding be arranged on described cultivation box, culture tube support and/or culture tube cartridge is placed in described cultivation box, culture tube placed by described culture tube support, described culture tube cartridge is used for carrying culture tube and nutrient solution in plant liquid suspension culture, and described top cover is provided with breather hole, and millipore filtration is installed at described breather hole place.Device of the present utility model can meet solid Semi-solid cell culture and also meet suspension culture, reaches high-throughout training mode.In addition, the utility model device can also enter the resistance test of plant, reaches high-throughout experiment demand, shorten experimental period, simultaneously in the real operation process in laboratory, the waste and old centrifuge tube that Eppendorf pipe can use by molecular biology uses, and decreases experimental cost.
Description
Technical field
The utility model relates to field of plant tissue culture technique, particularly, relates to a kind of high-throughput plant solid-liquid culture apparatus.
Background technology
Traditional plant tissue culture technique mainly adopts solid semi-solid agar substratum to cultivate in plant tissue culture bottle, under lab be widely used in transfer-gen plant as the cultivation of potato, Arabidopis thaliana, wheat etc. and screening, therefore a large amount of scientific experimentations is needed, if plant tissue culture pattern traditionally, need to buy a large amount of plant tissue culture bottles, want the agar of at substantial simultaneously, spend a large amount of time and manpower to carry out the cleaning of tissue culture flasks, take the space that laboratory is very large.In addition, also there are some shortcomings in some aspects in the method utilizing the in vitro tissue of solid agar medium to plant to cultivate, such as in culturing process, the nutritive ingredient of callus in process of growth of plant, the metabolic substd that plant tissue produces presents a Gradient distribution, and agar itself also has some not clear material compositions may have an impact to culture, thus cause the change of metabolism in plant tissue growth growth course to utilize liquid nutrient medium then can overcome this shortcoming, when plant be organized in grow in liquid nutrient medium time, can be cultivated by thin layer concussion or in substratum, ventilate to improve the supply of oxygen in substratum.The suspension culture of vegetable cell refers to vegetable cell or less cell mass to be suspended in liquid nutrient medium and cultivates, and can keep good dispersion state in culturing process.
Utility model content
In order to overcome the drawback of prior art, the utility model provides a kind of high-throughput plant solid-liquid culture apparatus.The utility model device had both met solid Semi-solid cell culture and had also met suspension culture, reached high-throughout training mode.The utility model device can also enter the resistance test of plant, reaches high-throughout experiment demand, shortens experimental period, decrease experimental cost.
The technical solution of the utility model is: a kind of high-throughput plant solid-liquid culture apparatus, comprise and cultivate box and top cover, described top cover can folding be arranged on described cultivation box, culture tube support and/or culture tube cartridge is placed in described cultivation box, culture tube placed by described culture tube support, described culture tube cartridge is used for carrying culture tube and nutrient solution in plant liquid suspension culture, and described top cover is provided with breather hole, and millipore filtration is installed at described breather hole place.
Preferably, silica gel sealing ring is provided with between described top cover and cultivation box.
Preferably, described cultivation box bottom is provided with dewatering outlet.
Preferably, described top cover is provided with buckle, with the clamping of described cultivation box.
Preferably, described top cover is provided with three breather holes.
Preferably, described dewatering outlet place is provided with polypropylene millipore filtration.
Preferably, described culture tube is Eppendorf pipe.
The beneficial effects of the utility model: device of the present utility model can meet solid Semi-solid cell culture and also meet suspension culture, reach high-throughout training mode.In addition, the utility model device can also enter the resistance test of plant, reaches high-throughout experiment demand, shorten experimental period, simultaneously in the real operation process in laboratory, the waste and old centrifuge tube that Eppendorf pipe can use by molecular biology uses, and decreases experimental cost.
Accompanying drawing explanation
Fig. 1 is the structural representation of a kind of embodiment of high-throughput plant solid-liquid culture apparatus in the utility model;
Fig. 2 is the culture tube supporting structure schematic diagram of carrying culture tube;
Fig. 3 is the structural representation of culture tube cartridge;
In figure: 1 cultivates box, 2 top covers, 3 buckles, 4 dewatering outlets, 5 culture tube cartridges, 6 culture tube support attachment straps, 7 breather holes.
Embodiment
By reference to the accompanying drawings the embodiment that the utility model provides is described in further detail:
A kind of high-throughput plant solid-liquid culture apparatus, comprise and cultivate box and top cover, described top cover can folding be arranged on described cultivation box, culture tube support and/or culture tube cartridge is placed in described cultivation box, culture tube placed by described culture tube support, described culture tube cartridge is used for carrying culture tube and nutrient solution in plant liquid suspension culture, and described top cover is provided with breather hole, and millipore filtration is installed at described breather hole place.
As shown in Figure 1, a kind of high-throughput plant solid-liquid culture apparatus, comprises top cover 2, culture tube support, culture tube, culture tube cartridge 5, cultivates box 1 and dewatering outlet 4.Top cover 2 is fastened on to be cultivated on box 1.Wherein culture tube rack bearing culture tube is placed on and cultivates in box 1, and culture tube cartridge 5 is applicable to carry culture tube and nutrient solution in plant liquid suspension culture, is placed on and cultivates in box 1.Cultivate in box and can hold sterilized water, the humidity be used in maintain box, dewatering outlet 4 is the lower right corner, box face on the right side of cultivation box, box top cover, cultivation box, culture tube support etc. are originally as fire-resistant nontoxic Polypropylene structural material, entirety can carry out 121 DEG C and carry out sterilising treatment, culture tube can adopt the Eppendorf of uncovered 1.5mL, 2.0mL, 5.0mL, 10mL and 50mL to manage; In liquid culture, Eppendorf pipe is modified to bottomless straight shape pipe, is furnished with the removable sponge pad suitable with Eppendorf pipe diameter simultaneously; Top cover 1 is provided with buckle 3, tight snap close can be carried out with cultivation box; Culture tube cartridge 5 is applicable to the growth characteristics test of plant under different nutrient solution condition, holds different nutrient solutions; Cultivation box top cover is provided with three and 0.22 μm of polypropylene millipore filtration is housed, in order to keep the fluidizing air in box body; Go out to be provided with 0.22 μm of polypropylene millipore filter in dewatering outlet, can constantly change.
Embodiment 1:
solid culture
Preparation MS solid medium, gets 0.75mL and is sub-packed in 1.5mL uncovered Eppendorf pipe, be positioned on the support of culture tube, be placed on culture tube support attachment strap 6,0.22 μm of polypropylene millipore filter is installed in dewatering outlet 4, covers box top cover 2, close top cover buckle 3, check top cover breather hole 7, entirety sterilizing 20min at 121 DEG C, after sterilizing terminates, the plant seed after inoculation sterilization or plant leaf, tile certain sterilized water bottom box body 1, cultivates under aseptic or open condition.
embodiment 2: liquid culture
Preparation MS liquid nutrient medium, gets 100mL and is poured in cultivation box body 1, managed by the 1.5mL uncovered Eppendorf that sponge pad is housed, be positioned on the support of culture tube, be placed on culture tube support attachment strap 6,0.22 μm of polypropylene millipore filter be installed in dewatering outlet 4, cover box top cover 2, close top cover buckle 3, check top cover breather hole 7, entirety sterilizing 20min at 121 DEG C, after sterilizing terminates, plant seedlings after inoculation sterilization, cultivates under aseptic or open condition.
embodiment 3: liquid grouping is cultivated
The MS liquid nutrient medium of preparation containing different N aCl concentration, get 10mL and be poured over culture tube cartridge 5, the 1.5mL uncovered Eppendorf that sponge pad is housed is managed, be positioned on the support of culture tube, be placed on culture tube support attachment strap 6,0.22 μm of polypropylene millipore filter is installed in dewatering outlet 4, cover box top cover 2, close top cover buckle 3, check top cover breather hole 7, entirety sterilizing 20min at 121 DEG C, after sterilizing terminates, plant seedlings after inoculation sterilization, cultivates, observes the growth effect of different nutrient solution to plant under aseptic or open condition.
In this description, the utility model is described with reference to its specific embodiment.But, still can make various amendment and conversion obviously and not deviate from spirit and scope of the present utility model.Therefore, specification sheets and accompanying drawing are regarded in an illustrative, rather than a restrictive.
Claims (7)
1. a high-throughput plant solid-liquid culture apparatus, it is characterized in that, comprise and cultivate box and top cover, described top cover can folding be arranged on described cultivation box, place culture tube support and/or culture tube cartridge in described cultivation box, culture tube placed by described culture tube support, described culture tube cartridge is used for carrying culture tube and nutrient solution in plant liquid suspension culture, described top cover is provided with breather hole, and millipore filtration is installed at described breather hole place.
2. high-throughput plant solid-liquid culture apparatus according to claim 1, is characterized in that, is provided with silica gel sealing ring between described top cover and cultivation box.
3. high-throughput plant solid-liquid culture apparatus according to claim 1 and 2, is characterized in that, described cultivation box bottom is provided with dewatering outlet.
4. high-throughput plant solid-liquid culture apparatus according to claim 1 and 2, it is characterized in that, described top cover is provided with buckle, with the clamping of described cultivation box.
5. high-throughput plant solid-liquid culture apparatus according to claim 1 and 2, it is characterized in that, described top cover is provided with three breather holes.
6. high-throughput plant solid-liquid culture apparatus according to claim 3, it is characterized in that, described dewatering outlet place is provided with polypropylene millipore filtration.
7. high-throughput plant solid-liquid culture apparatus according to claim 1 and 2, is characterized in that, described culture tube is Eppendorf pipe.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520663789.9U CN204939495U (en) | 2015-08-31 | 2015-08-31 | A kind of high-throughput plant solid-liquid culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201520663789.9U CN204939495U (en) | 2015-08-31 | 2015-08-31 | A kind of high-throughput plant solid-liquid culture apparatus |
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| Publication Number | Publication Date |
|---|---|
| CN204939495U true CN204939495U (en) | 2016-01-06 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201520663789.9U Expired - Fee Related CN204939495U (en) | 2015-08-31 | 2015-08-31 | A kind of high-throughput plant solid-liquid culture apparatus |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109415677A (en) * | 2016-06-21 | 2019-03-01 | 株式会社三博特 | Container is used in cell/bio-tissue conveying |
| CN114806832A (en) * | 2022-05-25 | 2022-07-29 | 深圳市华晨阳科技有限公司 | Small-size negative pressure infectivity pathology for experimental study preserves culture apparatus |
-
2015
- 2015-08-31 CN CN201520663789.9U patent/CN204939495U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109415677A (en) * | 2016-06-21 | 2019-03-01 | 株式会社三博特 | Container is used in cell/bio-tissue conveying |
| CN114806832A (en) * | 2022-05-25 | 2022-07-29 | 深圳市华晨阳科技有限公司 | Small-size negative pressure infectivity pathology for experimental study preserves culture apparatus |
| CN114806832B (en) * | 2022-05-25 | 2022-09-02 | 深圳市华晨阳科技有限公司 | Negative pressure infectious pathology preservation incubator for small-size experimental study |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170613 Address after: 733000 Gansu province Wuwei city Liangzhou District huangyangzhen Huangyanghe group eleven. Patentee after: Wuwei top ecological animal husbandry Co., Ltd. Address before: 730000 Gansu city of Lanzhou province West Lake Qilihe district along the 335 base Patentee before: Lanzhou Inst. of Animal Husbandary & Veterinary Medicine, Chinese Academy of Agr |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160106 Termination date: 20190831 |